Query：
学者姓名：陈倬
Refining：
Year
Type
Indexed by
Source
Complex
Co-Author
Language
Clean All
Abstract ：
Inconsistent nitrogen application rates across global croplands necessitate the adaptation of different genetic alleles to optimize nitrogen-use efficiency (NUE) in agriculture. In rice (Oryza sativa L.), different TEOSINTE BRANCHED 1, CYCLOIDEA AND PROLIFERATING CELL FACTOR 19 (OsTCP19) alleles contribute to the geographical adaptation to soil fertility. The nitrogen-sensitive allele OsTCP19-H is predominantly found in low-nitrogen regions due to its superior tillering and yield accumulation capabilities. Conversely, the nitrogen-insensitive allele OsTCP19-L is more common in nitrogen-sufficient regions, though the precise factors affecting this distribution remain unknown. Here, we report that high-nitrogen levels lead to lodging in an OsTCP19-dependent manner. Overexpression of this gene modifies plant architecture, enhancing lodging resistance in rice. Importantly, fine-tuning OsTCP19 expression can confer lodging resistance without a yield penalty. This is particularly important as it alleviates yield loss from lodging under high-nitrogen conditions and, intriguingly, can result in a substantial increase in plot yield when combined with dense planting strategies. Furthermore, the distinct nitrogen sensitivity of different OsTCP19 alleles allows for substantial improvement in grain yield and NUE under certain nitrogen conditions. Thus, our findings suggest that genetic manipulation of a single gene, OsTCP19, could allow flexible adaptation to diverse planting scenarios, maximizing genetic benefits based on local nitrogen availability. Increasing the expression of the transcription factor gene OsTCP19 improves lodging resistance in rice and allows for dense planting and improved nitrogen-use efficiency under high-nitrogen conditions.
Cite：
Copy from the list or Export to your reference management。
| GB/T 7714 | Liu, Yongqiang , Li, Weiwei , Wang, Xiaohan et al. Fine-tuning OsTCP19 expression offers broad adaptation scenarios for nitrogen-use efficiency improvement in rice [J]. | PLANT PHYSIOLOGY , 2025 , 199 (2) . |
| MLA | Liu, Yongqiang et al. "Fine-tuning OsTCP19 expression offers broad adaptation scenarios for nitrogen-use efficiency improvement in rice" . | PLANT PHYSIOLOGY 199 . 2 (2025) . |
| APA | Liu, Yongqiang , Li, Weiwei , Wang, Xiaohan , Li, Yaping , Zhu, Wenjun , Chen, Zhuo et al. Fine-tuning OsTCP19 expression offers broad adaptation scenarios for nitrogen-use efficiency improvement in rice . | PLANT PHYSIOLOGY , 2025 , 199 (2) . |
| Export to | NoteExpress RIS BibTex |
Version ：
Abstract ：
Empirical breeding efforts targeting cold tolerance and ideal plant architecture have significantly improved yield and facilitated the geographic expansion of japonica rice cultivation. However, the genetic drivers and underlying molecular mechanisms of these traits remain insufficiently understood. Here, we identify Plant Height 8 (PH8) as a key gene regulating both plant stature and cold stress response in rice. Genome wide association analysis (GWAS), supported by functional validation, shows that loss of PH8 reduces plant height without affecting other agronomic traits. Notably, we found that PH8 also negatively regulates cold tolerance. A prevalent haplotype, PH8Hap.0, exhibits reduced PH8 expression due to natural variation in its promoter region, resulting in shorter plants and enhanced cold tolerance. Selective sweep and geographic distribution analyses indicate that PH8Hap.0 originated in high-latitude regions and underwent strong directional selection during modern japonica improvement. Functional assays demonstrate that PH8 enhances cold tolerance via improved reactive oxygen species (ROS) scavenging by repressing APX2, an antioxidant gene involved in ROS detoxification. Our findings reveal PH8 as a dual regulator of plant architecture and cold stress adaptation, and highlight PH8Hap.0 as a historically selected allele that contributed to the climatic adaptation and geographical expansion of japonica rice.
Keyword ：
Cold tolerance Cold tolerance GWAS GWAS Plant height Plant height Rice Rice Selection Selection
Cite：
Copy from the list or Export to your reference management。
| GB/T 7714 | Chen, Cheng , Zhang, Xia , Chen, Jialin et al. Natural Variation of PH8 Allele Improves Architecture and Cold Tolerance in Rice [J]. | RICE , 2025 , 18 (1) . |
| MLA | Chen, Cheng et al. "Natural Variation of PH8 Allele Improves Architecture and Cold Tolerance in Rice" . | RICE 18 . 1 (2025) . |
| APA | Chen, Cheng , Zhang, Xia , Chen, Jialin , Xu, Mingjia , Zhao, Weiying , Wang, Yangkai et al. Natural Variation of PH8 Allele Improves Architecture and Cold Tolerance in Rice . | RICE , 2025 , 18 (1) . |
| Export to | NoteExpress RIS BibTex |
Version ：
Abstract ：
Ubiquitin-mediated protein modification by E3 ligases is crucial for plant stress responses. Here, we demonstrate that the RING-type E3 ligase RhNIRF1 physically interacts with and ubiquitinates the NAC-domain transcription factor RhNAC31, establishing a regulatory module that governs drought tolerance in rose (Rosa hybrida). Silencing of RhNAC31 resulted in decreased dehydration tolerance, whereas its overexpression conferred enhanced photosynthetic capacity concomitant with reduced relative oxygen species accumulation. Notably, RhNIRF1 transcript levels were significantly downregulated under drought stress, while RhNAC31 exhibited an opposite trend. In vitro ubiquitination assays confirmed that the RING domain of RhNIRF1 possesses intrinsic E3 ligase activity specifically targeting RhNAC31 for polyubiquitination. Moreover, RhNAC31 directly binds to various stress-related genes in rose, including RhABI1 and RhANAC083, functioning as a transcriptional activator during dehydration responses. Luciferase assays demonstrated that RhNIRF1 accelerates the degradation of RhNAC31, thereby modulating the binding ability of downstream genes. Our findings highlight the RhNIRF1-RhNAC31 module as a novel molecular switch at the post-translational level for improving drought stress tolerance in rose plants.
Keyword ：
drought stress drought stress E3 ubiquitin ligases E3 ubiquitin ligases RhNAC31 RhNAC31 RhNIRF1 RhNIRF1 Rosa hybrida Rosa hybrida
Cite：
Copy from the list or Export to your reference management。
| GB/T 7714 | Fu, Lufeng , Tang, Guohao , Ni, Xueting et al. RhNIRF1-mediated ubiquitination of RhNAC31 affects drought tolerance by regulating stress-related genes in Rosa hybrida [J]. | PLANT JOURNAL , 2025 , 122 (4) . |
| MLA | Fu, Lufeng et al. "RhNIRF1-mediated ubiquitination of RhNAC31 affects drought tolerance by regulating stress-related genes in Rosa hybrida" . | PLANT JOURNAL 122 . 4 (2025) . |
| APA | Fu, Lufeng , Tang, Guohao , Ni, Xueting , Zeng, Wen , Shi, Yaru , Geng, Lifang et al. RhNIRF1-mediated ubiquitination of RhNAC31 affects drought tolerance by regulating stress-related genes in Rosa hybrida . | PLANT JOURNAL , 2025 , 122 (4) . |
| Export to | NoteExpress RIS BibTex |
Version ：
Abstract ：
The banana ( Musa spp.) peel undergoes rapid softening during ripening, leading to finger drop and a shortened shelf life. The regulatory mechanism behind this process remains to be elucidated. In this study, we confirmed the role of peel softening in banana finger drop and uncovered the underlying transcriptional regulatory network. Cell wall-related (CWR) genes were substantially upregulated in both the peel and finger drop zone during ethylene-induced ripening. Transcriptome analysis and genome-wide profiling of chromatin accessibility and transcription factor (TF) binding revealed that two key regulators of fruit ripening, Musa acuminata NAC-like, Activated by apetala3/Pistillata1 (MaNAP1) and MaMADS1, regulate CWR genes by directly binding to their promoters or by targeting other ripening-related TFs to form a hierarchical regulatory network. Notably, MaNAP1 and MaMADS1 were directly targeted by ETHYLENE INSENSITIVE3 (MaEIN3), and MaNAP1 and MaMADS1 associated with tissue-specific histone modifications, enabling them to integrate MaEIN3-mediated ethylene signaling and undergo epigenetic regulation. Overexpression of MaNAP1, MaMADS1, or other identified regulatory TFs upregulated CWR genes and promoted peel softening. Our findings unveil a MaNAP1-MaMADS1-centered regulatory cascade governing banana peel softening and finger drop, offering potential targets for enhancing banana texture and shelf life.
Cite：
Copy from the list or Export to your reference management。
| GB/T 7714 | Li, Hua , Chen, Zhuo , Zhu, Wenjun et al. The MaNAP1-MaMADS1 transcription factor module mediates ethylene-regulated peel softening and ripening in banana [J]. | PLANT CELL , 2024 , 37 (1) . |
| MLA | Li, Hua et al. "The MaNAP1-MaMADS1 transcription factor module mediates ethylene-regulated peel softening and ripening in banana" . | PLANT CELL 37 . 1 (2024) . |
| APA | Li, Hua , Chen, Zhuo , Zhu, Wenjun , Ni, Xueting , Wang, Junru , Fu, Lufeng et al. The MaNAP1-MaMADS1 transcription factor module mediates ethylene-regulated peel softening and ripening in banana . | PLANT CELL , 2024 , 37 (1) . |
| Export to | NoteExpress RIS BibTex |
Version ：
Abstract ：
【目的】NAC(NAM, ATAF and CUC)参与植物生长发育和多种逆境胁迫响应过程的调控。本文旨在鉴定和研究对矮牵牛生长发育和逆境胁迫响应的关键NAC成员，为优质抗逆矮牵牛育种提供基因资源。【方法】以腋生矮牵牛（Petunia axillaris）基因组为参考基因组，利用矮牵牛花器官衰老过程、烟草脆裂病毒（Tobacco rattle virus,TRV）侵染、低磷、低温、NaCl、铜离子和干旱胁迫处理后的转录组数据，分析矮牵牛NAC基因（PaNACs）差异表达情况，并对差异表达PaNACs的启动子顺式作用元件及转录因子结合位点进行分析。利用实时荧光定量PCR验证了部分差异表达PaNACs在矮牵牛花衰老过程中的表达情况，并预测了差异表达PaNACs编码蛋白的潜在靶基因。【结果】鉴定的131个PaNAC基因中，59个（45.04%）被鉴定为花器官衰老和逆境胁迫响应过程中的差异表达基因。PaNAC72、 PaNAC22、 PaNAC29、 PaNAC40、 PaNAC2、 PaNAC90、 PaNAC83、 PaNAC56、 PaNAC36和PaNAC35在至少3个生物学过程响应中差异表达显著，其中拟南芥衰老关键基因AtNAP的直系同源基因PaNAC29在花器官衰老过程和低温、低磷、铜离子胁迫逆境处理中显著上调表达；PaNAC72在除受铜离子胁迫外的所有6种处理中表达差异显著；PaNAC22在花器官衰老过程和低温和低磷胁迫中上调表达，在铜离子和干旱逆境下调表达。启动子分析结果显示这10个PaNAC启动子区域存在多种逆境胁迫响应相关元件，且大量响应衰老和逆境胁迫的差异表达基因的启动子区域存在NAC的结合位点。【结论】PaNACs广泛参与矮牵牛生长发育及逆境胁迫响应，其中PaNAC29可能是花衰老关键的正调控因子，PaNAC72广泛响应多种逆境胁迫。
Keyword ：
NAC NAC 生物信息学 生物信息学 矮牵牛 矮牵牛 胁迫 胁迫 衰老 衰老 转录组 转录组
Cite：
Copy from the list or Export to your reference management。
| GB/T 7714 | 杨应杰 , 张付昆 , 穆静怡 et al. 矮牵牛花瓣衰老和逆境胁迫响应相关NAC基因的鉴定与分析 [J]. | 福建农业学报 , 2024 , 39 (06) : 700-710 . |
| MLA | 杨应杰 et al. "矮牵牛花瓣衰老和逆境胁迫响应相关NAC基因的鉴定与分析" . | 福建农业学报 39 . 06 (2024) : 700-710 . |
| APA | 杨应杰 , 张付昆 , 穆静怡 , 付鲁峰 , 陈倬 , 李华 et al. 矮牵牛花瓣衰老和逆境胁迫响应相关NAC基因的鉴定与分析 . | 福建农业学报 , 2024 , 39 (06) , 700-710 . |
| Export to | NoteExpress RIS BibTex |
Version ：
Abstract ：
Banana (Musa spp.) fruits, as typical tropical fruits, are cold sensitive, and lower temperatures can disrupt cellular compartmentalization and lead to severe browning. How tropical fruits respond to low temperature compared to the cold response mechanisms of model plants remains unknown. Here, we systematically characterized the changes in chromatin accessibility, histone modifications, distal cis-regulatory elements, transcription factor binding, and gene expression levels in banana peels in response to low temperature. Dynamic patterns of cold-induced transcripts were generally accompanied by concordant chromatin accessibility and histone modification changes. These upregulated genes were enriched for WRKY binding sites in their promoters and/or active enhancers. Compared to banana peel at room temperature, large amounts of banana WRKYs were specifically induced by cold and mediated enhancer-promoter interactions regulating critical browning pathways, including phospholipid degradation, oxidation, and cold tolerance. This hypothesis was supported by DNA affinity purification sequencing, luciferase reporter assays, and transient expression assay. Together, our findings highlight widespread transcriptional reprogramming via WRKYs during banana peel browning at low temperature and provide an extensive resource for studying gene regulation in tropical plants in response to cold stress, as well as potential targets for improving cold tolerance and shelf life of tropical fruits. The core WRKY module binds to the promoters and/or enhancers of cold-induced genes as a critical regulator during banana peel browning upon exposure to low temperature.
Cite：
Copy from the list or Export to your reference management。
| GB/T 7714 | Zhu, Wenjun , Li, Hua , Dong, Pengfei et al. Low temperature-induced regulatory network rewiring via WRKY regulators during banana peel browning [J]. | PLANT PHYSIOLOGY , 2023 , 193 (1) : 855-873 . |
| MLA | Zhu, Wenjun et al. "Low temperature-induced regulatory network rewiring via WRKY regulators during banana peel browning" . | PLANT PHYSIOLOGY 193 . 1 (2023) : 855-873 . |
| APA | Zhu, Wenjun , Li, Hua , Dong, Pengfei , Ni, Xueting , Fan, Minlei , Yang, Yingjie et al. Low temperature-induced regulatory network rewiring via WRKY regulators during banana peel browning . | PLANT PHYSIOLOGY , 2023 , 193 (1) , 855-873 . |
| Export to | NoteExpress RIS BibTex |
Version ：
Abstract ：
The fundamental biological function of nucleoside diphosphate kinase (NDK) is to catalyze the reversible exchange of the gamma-phosphate between nucleoside triphosphate (NTP) and nucleoside diphosphate (NDP). This kinase also has functions that extend beyond its canonically defined enzymatic role as a phosphotransferase. However, the role of NDK in filamentous fungi, especially in Aspergillus flavus (A. flavus), is not yet known. Here we report that A. flavus has two NDK-encoding gene copies as assessed by qPCR. Using gene-knockout and complementation experiments, we found that AfNDK regulates spore and sclerotia development and is involved in plant virulence as assessed in corn and peanut seed-based assays. An antifungal test with the inhibitor azidothymidine suppressed AfNDK activity in vitro and prevented spore production and sclerotia formation in A. flavus, confirming AfNDK's regulatory functions. Crystallographic analysis of AfNDK, coupled with site-directed mutagenesis experiments, revealed three residues (Arg-104, His-117, and Asp-120) as key sites that contribute to spore and sclerotia development. These results not only enrich our knowledge of the regulatory role of this important protein in A. flavus, but also provide insights into the prevention of A. flavus infection in plants and seeds, as well as into the structural features relevant for future antifungal drug development.
Keyword ：
Aspergillus Aspergillus Aspergillus flavus Aspergillus flavus azidothymidine (AZT) azidothymidine (AZT) crystal structure crystal structure developmental factor developmental factor fungi fungi inhibitor inhibitor microbiology microbiology nucleoside diphosphate kinase (NDK) nucleoside diphosphate kinase (NDK) pathology pathology protein kinase protein kinase recombinant protein expression recombinant protein expression sclerotium sclerotium spore spore sporulation sporulation
Cite：
Copy from the list or Export to your reference management。
| GB/T 7714 | Wang, Yu , Wang, Sen , Nie, Xinyi et al. Molecular and structural basis of nucleoside diphosphate kinase-mediated regulation of spore and sclerotia development in the fungus Aspergillus flavus [J]. | JOURNAL OF BIOLOGICAL CHEMISTRY , 2019 , 294 (33) : 12415-12431 . |
| MLA | Wang, Yu et al. "Molecular and structural basis of nucleoside diphosphate kinase-mediated regulation of spore and sclerotia development in the fungus Aspergillus flavus" . | JOURNAL OF BIOLOGICAL CHEMISTRY 294 . 33 (2019) : 12415-12431 . |
| APA | Wang, Yu , Wang, Sen , Nie, Xinyi , Yang, Kunlong , Xu, Peng , Wang, Xiuna et al. Molecular and structural basis of nucleoside diphosphate kinase-mediated regulation of spore and sclerotia development in the fungus Aspergillus flavus . | JOURNAL OF BIOLOGICAL CHEMISTRY , 2019 , 294 (33) , 12415-12431 . |
| Export to | NoteExpress RIS BibTex |
Version ：
Export
| Results： |
Selected to |
| Format： |
