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学者姓名：郑国华
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Abstract ：
油茶是中国重要的木本油料树种，具有较高的利用价值。本研究利用SRAP分子标记对10个福建省主栽普通油茶优良无性系进行分析，探讨其遗传差异，结果表明：从256对SRAP引物组合中筛选出12对引物对10个优良无性系进行PCR扩增，共获得186个条带，其中多态性条带166个，多态性条带的比率为89.2%。EM-2/ME-4引物组合扩增的条带多态性最高，为100%；多态性信息量最小值和最大值分别为0.280和0.394，多态性信息量平均值为0.330。10个优良无性系遗传相似系数最小值和最大值分别为0.591 4和0.774 2，遗传相似系数平均值为0.670 8。聚类分析表明，遗传相似系数为0.677 5处，10个普通油茶优良无性系可以划为4大类：闽43和闽46聚为第Ⅰ类，闽48、闽49、闽53聚为第Ⅱ类，闽50聚为第Ⅲ类，闽54、闽56、闽60、闽62聚为第Ⅳ类。这10个优良无性系之间存在着较高的遗传多样性水平，研究结果为普通油茶杂交育种和分子育种工作的开展提供参考。
Keyword ：
SRAP SRAP 无性系 无性系 普通油茶 普通油茶 遗传差异 遗传差异
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| GB/T 7714 | 王庭雄 , 李祥胜 , 梁诗强 et al. 福建省主栽普通油茶优良无性系遗传差异的SRAP分析 [J]. | 分子植物育种 , 2024 , 22 (17) : 5663-5669 . |
| MLA | 王庭雄 et al. "福建省主栽普通油茶优良无性系遗传差异的SRAP分析" . | 分子植物育种 22 . 17 (2024) : 5663-5669 . |
| APA | 王庭雄 , 李祥胜 , 梁诗强 , 叶树涛 , 吕欢欢 , 陈辉 et al. 福建省主栽普通油茶优良无性系遗传差异的SRAP分析 . | 分子植物育种 , 2024 , 22 (17) , 5663-5669 . |
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‘油榛’是福建省建瓯市广泛种植的优良农家品种之一。本研究利用SSR分子标记对‘油榛’栽培群体进行遗传多样性分析，探索其变异情况，结果表明：由10个SSR引物组合对锥栗农家品种‘油榛’栽培群体的30个植株进行PCR扩增，共计扩增出26个条带，多态性条带26个，多态性条带占比100%,30个植株的遗传相似性系数在0.115 4～1.000 0之间，平均为0.877 2，表明锥栗农家品种‘油榛’具有较高的遗传多样性。为了解‘油榛’栽培群体30个植株的亲缘关系，对30个植株进行聚类分析，其中YZ5、YZ1单独各聚为一类，其余28个植株聚为一类。研究结果为在锥栗农家品种栽培群体中进行优树选择提供了理论依据。
Keyword ：
SSR SSR 农家品种 农家品种 油榛 油榛 遗传多样性 遗传多样性 锥栗 锥栗
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| GB/T 7714 | 叶树涛 , 方周 , 李颖林 et al. 锥栗农家品种‘油榛’栽培群体的SSR变异分析 [J]. | 分子植物育种 , 2024 , 22 (05) : 1508-1513 . |
| MLA | 叶树涛 et al. "锥栗农家品种‘油榛’栽培群体的SSR变异分析" . | 分子植物育种 22 . 05 (2024) : 1508-1513 . |
| APA | 叶树涛 , 方周 , 李颖林 , 吴钧剑 , 郑国华 , 陈辉 et al. 锥栗农家品种‘油榛’栽培群体的SSR变异分析 . | 分子植物育种 , 2024 , 22 (05) , 1508-1513 . |
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Methyleugenol is widely found in essential oils from aromatic plants, attracting insects for pollination and possessing antimicrobial and antioxidant features. Methyleugenol content in the essential oil from Melaleuca bracteata leaves is 90.46 %, making it an excellent material for assessing methyleugenol's biosynthetic pathway. Eugenol synthase (EGS) and eugenol-o-methyltransferase (EOMT) represent key genes in methyleugenol's biosynthetic pathway but their respective functions are unclear. In this study, overexpression and virus-induced gene silencing (VIGS) techniques were applied to investigate the major regulatory gene among MbEGS1, MbEGS2, MbEGS3, and MbEGS4 in the biosynthesis pathway of methyleugenol. We also aimed to examine the coordination mechanism between EGS and EOMT in methyleugenol production by M. bracteata. The data revealed MbEGS4 transcription and methyleugenol content were most significantly increased after overexpression of the MbEGS1-4 gene in M. bracteata and Fragaria vesca. Conversely, MbEGS4 transcription and methyleugenol content were most significantly reduced after MbEGS1-4 gene silencing by VIGS in M. bracteata. These results underscore a major regulatory role for the MbEGS4 gene in the biosynthetic pathway of methyleugenol in M. bracteata. Remarkably, upon overexpression and silencing of MbEOMTs, the transcription levels of MbEGSs were notably upregulated, with MbEGS4 showing the highest increase. Collectively, these findings suggested the presence of a specific transcription factor bound to the promoters of MbEGS and MbEOMT, inducing their expression for the synergistic and efficient production of methyleugenol in M. bracteata.
Keyword ：
Biosynthesis Biosynthesis Eugenol synthase gene Eugenol synthase gene Methyleugenol Methyleugenol Phenylpropene Phenylpropene
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| GB/T 7714 | Xiang, Xuwen , Song, Keke , Lin, Yongsheng et al. Mechanistic analysis of the coordination between MbEGS4 and MbEOMT in methyleugenol synthesis by Melaleuca bracteata F. Muell [J]. | INDUSTRIAL CROPS AND PRODUCTS , 2024 , 222 . |
| MLA | Xiang, Xuwen et al. "Mechanistic analysis of the coordination between MbEGS4 and MbEOMT in methyleugenol synthesis by Melaleuca bracteata F. Muell" . | INDUSTRIAL CROPS AND PRODUCTS 222 (2024) . |
| APA | Xiang, Xuwen , Song, Keke , Lin, Yongsheng , You, Jingnan , Xu, Yan , Niu, Xianqian et al. Mechanistic analysis of the coordination between MbEGS4 and MbEOMT in methyleugenol synthesis by Melaleuca bracteata F. Muell . | INDUSTRIAL CROPS AND PRODUCTS , 2024 , 222 . |
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Advancement of the oil tea industry requires the development of high-yielding and superior-quality varieties of Camellia oleifera, a major oilseed crop. However, traditional breeding methods, hampered by lengthy cycles and low selection accuracy, significantly constrain the breeding process. Identifying single nucleotide polymorphisms (SNPs) associated with target traits, and applying molecular marker-assisted selection (MAS) for these traits, can thereby shorten the breeding cycles and amplify the breeding efficiency. In this study, we utilized the hexaploid C. oleifera as the reference genome to identify high-quality SNPs and constructed a high-density genetic linkage map of C. oleifera that spanned 1566.733 cM, included 3097 SNPs, and was anchored to 15 linkage groups. Using interval mapping, we localized quantitative trait loci (QTLs) for 11 juvenile traits in C. oleifera, identifying 15 QTLs for growth traits and 24 QTLs for leaf traits, including 4 stable QTLs. The logarithm of odds (LOD) scores for individual QTLs ranged from 3.48 to 14.62, explaining 9.86-48.61% of the phenotypic variance. We further identified 2 SNPs associated with growth traits (marker11-951 and marker12-68) and 10 SNPs associated with leaf traits (marker11-276, marker11-410, marker11-560, marker13-16, marker13-39, marker13-110, marker13-731, marker14-701, marker14-910, and marker14-1331). These results provide valuable insights into the genetic mapping of key traits in C. oleifera and will contribute to the development of new varieties with high yield and superior quality in the future.
Keyword ：
Camellia oleifera Camellia oleifera genetic linkage map genetic linkage map juvenile traits juvenile traits QTL QTL SNP SNP
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| GB/T 7714 | Ye, Ling , Li, Yu , Liu, Yanxuan et al. Genetic Linkage Map Construction and QTL Mapping for Juvenile Leaf and Growth Traits in Camellia oleifera [J]. | AGRONOMY-BASEL , 2024 , 14 (12) . |
| MLA | Ye, Ling et al. "Genetic Linkage Map Construction and QTL Mapping for Juvenile Leaf and Growth Traits in Camellia oleifera" . | AGRONOMY-BASEL 14 . 12 (2024) . |
| APA | Ye, Ling , Li, Yu , Liu, Yanxuan , Zhou, Lexin , Li, Jia'ni , Liang, Tian et al. Genetic Linkage Map Construction and QTL Mapping for Juvenile Leaf and Growth Traits in Camellia oleifera . | AGRONOMY-BASEL , 2024 , 14 (12) . |
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Abstract ：
为探究‘油榛’栽培群体不同个体间的变异情况，筛选优良单株。以福建省建瓯市农家品种‘油榛’ 30株个体为材料，对其进行农艺性状和栗疫病抗性的测定，进一步作变异分析、方差分析、相关性分析，并对主要性状进行标准化排序，评选优良单株。结果表明：各性状平均变异系数介于6.02%～21.28%之间，坚果高度变异系数最小，果苞厚变异系数最大。方差分析表明各性状都存在显著（P<0.05）或极显著（P<0.01）差异。经标准化排序最终筛选出果实品质优良单株为YZ7、 YZ19、YZ28、 YZ21、 YZ4，抗病性优良单株为YZ5、 YZ30。农家品种‘油榛’栽培群体变异丰富，选择潜力较大，研究结果可为锥栗丰产优质新品种选育提供参考。
Keyword ：
优树选择 优树选择 农家品种 农家品种 栗疫病抗性 栗疫病抗性 ‘油榛’ ‘油榛’ 锥栗 锥栗
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| GB/T 7714 | 张晶 , 李嘉 , 李煜 et al. 锥栗农家品种‘油榛’栽培群体的性状变异及选择 [J]. | 森林与环境学报 , 2023 , 43 (05) : 540-547 . |
| MLA | 张晶 et al. "锥栗农家品种‘油榛’栽培群体的性状变异及选择" . | 森林与环境学报 43 . 05 (2023) : 540-547 . |
| APA | 张晶 , 李嘉 , 李煜 , 王鹏博 , 陈辉 , 郑国华 . 锥栗农家品种‘油榛’栽培群体的性状变异及选择 . | 森林与环境学报 , 2023 , 43 (05) , 540-547 . |
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Abstract ：
【目的】比较分析锥栗不同农家品种在栗疫病抗性、果实主要表型性状及营养成分等方面的差异情况，筛选出对栗疫病有较强抗性且果实品质表现优良的农家品种，从而为锥栗的良种选育提供参考依据。【方法】对锥栗疫病的病原菌进行形态学和核糖体DNA-ITS序列鉴定，在此基础上，对17个锥栗农家品种栗疫病抗性和果实表型性状指标（包括栗疫病染病率、果质量、淀粉含量等15个指标）进行测定，并对锥栗疫病抗性和果实表型性状进行变异分析，通过标准化分析选择优良品种。【结果】栗疫病抗性最强的农家品种为‘小尖嘴’，其染病指数为23.55；栗疫病抗性最弱的农家品种为‘处暑红’，其染病指数为59。17个农家品种在15个表型性状上存在不同程度的变异，果实营养成分的平均变异系数为5.56%，其染病率的平均变异系数为18.29%，果实表型性状指标的平均变异系数为13.33%。17个农家品种的栗疫病抗性和果实表型性状均存在显著差异（P≤0.05），除淀粉含量（P=0.275）外的15个表型性状指标在17个农家品种之间均存在极显著差异（P≤0.01）；栗疫病染病率和果实中蔗糖、可溶性蛋白的含量之间均呈现负相关，其相关系数分别为-0.292(P≤0.05)和-0.415(P≤0.01)。通过对所测数据进行标准化分析，从17个农家品种中筛选出了‘大包榛’‘白露仔’‘黄榛’这3个优良农家品种。聚类分析结果表明，17个农家品种可分为3大类：‘牛角仔’（中熟）单独分为第1类；‘牛角仔’（晚熟）‘油桐仔’‘处暑红’‘红仔榛’‘乌榛’聚为第2类；其余11个农家品种聚为第3类。【结论】不同农家品种的果实品质不同，对于栗疫菌侵染所表现出的抗性也存在显著差异。以栗疫病抗性和重要经济性状为选择目标，筛选出了3个栗疫病抗性较强且果实品质优良的农家品种。
Keyword ：
农家品种 农家品种 果实品质 果实品质 栗疫病抗性 栗疫病抗性 良种选择 良种选择 锥栗 锥栗
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| GB/T 7714 | 叶树涛 , 顾光仕 , 李煜 et al. 锥栗农家品种栗疫病抗性及果实表型性状的变异分析与联合选择 [J]. | 经济林研究 , 2022 , 40 (01) : 82-94 . |
| MLA | 叶树涛 et al. "锥栗农家品种栗疫病抗性及果实表型性状的变异分析与联合选择" . | 经济林研究 40 . 01 (2022) : 82-94 . |
| APA | 叶树涛 , 顾光仕 , 李煜 , 方周 , 张晶 , 杨金辉 et al. 锥栗农家品种栗疫病抗性及果实表型性状的变异分析与联合选择 . | 经济林研究 , 2022 , 40 (01) , 82-94 . |
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为探究普通油茶杂交亲本选配和授粉树配置对果实性状的影响，以4个普通油茶优良无性系为材料，通过不完全双列杂交获得12个组合的杂交果实，分析杂交组合可配性和杂交果实性状变异，估算配合力，探讨杂交果实性状间的相关性。结果表明，以‘闽43’‘闽60’为母本的杂交组合、‘闽50’‘闽54’为父本的杂交组合的平均亲和指数较高。杂交果实性状的变异系数在7.00%～59.58%之间，单果籽粒数变异系数最大，其次是单果鲜籽重，种仁含油率变异系数最小。12号杂交组合在单果鲜籽重、单果鲜籽率和种仁含油率方面均表现较好。除种仁含油率外，杂交果实性状间呈不同程度的相关性。综合杂交组合可配性、果实性状变异、配合力效应值，筛选出‘闽43’和‘闽60’为优良母本，‘闽50’和‘闽54’为优良父本，优良杂交组合为12号（‘闽60’ב闽54’）。
Keyword ：
亲本选配 亲本选配 变异分析 变异分析 无性系 无性系 普通油茶 普通油茶 有性杂交 有性杂交 果实性状 果实性状
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| GB/T 7714 | 梁恬 , 李祥胜 , 李煜 et al. 普通油茶优良无性系有性杂交果实性状变异分析 [J]. | 森林与环境学报 , 2022 , 42 (02) : 166-173 . |
| MLA | 梁恬 et al. "普通油茶优良无性系有性杂交果实性状变异分析" . | 森林与环境学报 42 . 02 (2022) : 166-173 . |
| APA | 梁恬 , 李祥胜 , 李煜 , 吕欢欢 , 郑国华 , 陈辉 . 普通油茶优良无性系有性杂交果实性状变异分析 . | 森林与环境学报 , 2022 , 42 (02) , 166-173 . |
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百香果产量问题是当前百香果产业面临的突出问题,尤其是上半年产量是决定果农收益的关键。本文就如何提高黄金百香果上半年产量,提出了一些栽培方法,涉及大苗定植、科学密植、高垅高墩种植等栽培技术,主要目的是提早增加单位面积的结果枝数量,从而提高早期产量,为广大百香果种植户实现早产丰产提供技术参考。
Keyword ：
早产丰产 早产丰产 栽培技术 栽培技术 黄金百香果 黄金百香果
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| GB/T 7714 | 杜丽君 , 林秀香 , 林秋金 et al. 黄金百香果早产丰产栽培技术 [J]. | 福建热作科技 , 2022 , 47 (01) : 57-60 . |
| MLA | 杜丽君 et al. "黄金百香果早产丰产栽培技术" . | 福建热作科技 47 . 01 (2022) : 57-60 . |
| APA | 杜丽君 , 林秀香 , 林秋金 , 李永裕 , 江莉 , 郑国华 et al. 黄金百香果早产丰产栽培技术 . | 福建热作科技 , 2022 , 47 (01) , 57-60 . |
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Actinidiaceae, an economically important plant family, includes the Actinidia, Clematoclethra and Saurauia genus. Kiwifruit, with remarkably high vitamin C content, is an endemic species widely distributed in China with high economic value. Although many Actinidiaceae chloroplast genomes have been reported, few complete mitogenomes of Actinidiaceae have been studied. Here, complete circular mitogenomes of the four kiwifruit species and Saurauia tristyla were assembled. Codon usage, sequence repeats, RNA editing, gene transfers, selective pressure, and phylogenetic relationships in the four kiwifruit species and S. tristyla were comparatively analyzed. This research will contribute to the study of phylogenetic relationships within Actiniaceae and molecular barcoding in kiwifruit.
Keyword ：
Actinidiaceae Actinidiaceae comparative analysis comparative analysis mitogenome mitogenome phylogenetic analysis phylogenetic analysis
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| GB/T 7714 | Yang, Jun , Ling, Chengcheng , Zhang, Huamin et al. A Comparative Genomics Approach for Analysis of Complete Mitogenomes of Five Actinidiaceae Plants [J]. | GENES , 2022 , 13 (10) . |
| MLA | Yang, Jun et al. "A Comparative Genomics Approach for Analysis of Complete Mitogenomes of Five Actinidiaceae Plants" . | GENES 13 . 10 (2022) . |
| APA | Yang, Jun , Ling, Chengcheng , Zhang, Huamin , Hussain, Quaid , Lyu, Shiheng , Zheng, Guohua et al. A Comparative Genomics Approach for Analysis of Complete Mitogenomes of Five Actinidiaceae Plants . | GENES , 2022 , 13 (10) . |
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Juice sac granulation (a physiological disorder) leads to large postharvest losses of pomelo (Citrus maxima). Previous studies have shown that juice sac granulation is closely related to lignin accumulation, while the molecular mechanisms underlying this disorder remain elusive in pomelo. Our results showed that the lignin content in NC (near the core) and FC (far away from the core) juice sacs overall increased from 157 DPA (days post anthesis) to 212 DPA and reached a maximum at 212 DPA. Additionally, the lignin content of NC juice sacs was higher than that of FC juice sacs. In this study, we used transcriptome-based weighted gene co-expression network analysis (WGCNA) to address how lignin formation in NC and FC juice sacs is generated during the development of pomelo. After data assembly and bioinformatic analysis, we found a most correlated module (black module) to the lignin content, then we used the 11 DEGs in this module as hub genes for lignin biosynthesis. Among these DEGs, PAL (phenylalanine ammonia lyase), HCT (hydroxycinnamoyl-CoA shikimate/quinate hydroxycinnamoyl transferase), 4CL2 (4-coumarate: CoA ligase), C4H (cinnamate 4-hydroxylase), C3 ' H (p-coumarate 3-hydroxylase), and CCoAOMT1 (caffeoyl CoA 3-Omethyltransferase) were the most distinct DEGs in granulated juice sacs. Co-expression analysis revealed that the expression patterns of several transcription factors such as MYB, NAC, OFP6, and bHLH130 are highly correlated with lignin formation. In addition, the expression patterns of the DEGs related to lignin biosynthesis and transcription factors were validated by qRT-PCR, and the results were highly concordant with the RNA-seq results. These results would be beneficial for further studies on the molecular mechanism of lignin accumulation in pomelo juice sacs and would help with citrus breeding.
Keyword ：
granulation granulation lignin lignin pomelo pomelo transcriptome transcriptome WGCNA WGCNA
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| GB/T 7714 | Li, Xiaoting , Huang, Hantang , Rizwan, Hafiz Muhammad et al. Transcriptome Analysis Reveals Candidate Lignin-Related Genes and Transcription Factors during Fruit Development in Pomelo (Citrus maxima) [J]. | GENES , 2022 , 13 (5) . |
| MLA | Li, Xiaoting et al. "Transcriptome Analysis Reveals Candidate Lignin-Related Genes and Transcription Factors during Fruit Development in Pomelo (Citrus maxima)" . | GENES 13 . 5 (2022) . |
| APA | Li, Xiaoting , Huang, Hantang , Rizwan, Hafiz Muhammad , Wang, Naiyu , Jiang, Jingyi , She, Wenqin et al. Transcriptome Analysis Reveals Candidate Lignin-Related Genes and Transcription Factors during Fruit Development in Pomelo (Citrus maxima) . | GENES , 2022 , 13 (5) . |
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