Query：
学者姓名：杨超
Refining：
Year
Type
Indexed by
Source
Complex
Co-Author
Language
Clean All
Abstract ：
Bougainvillea is valued for its ornamental and medicinal properties, but its current propagation primarily relies on cuttings, which suffer from low efficiency. Although tissue culture offers faster propagation and is not limited by seasonal properties, the technical system remains underdeveloped. This study applies the Response Surface Methodology (RSM) to optimize the axillary bud induction and rooting stages of Bougainvillea tissue culture. By fitting a multivariate quadratic regression model to determine the relationship between factors and response values, the study aimed to refine the tissue culture system for Bougainvillea. The main findings of this study are as follows: (1) In the axillary bud induction stage, the optimal induction medium was MS (Murashige and Skoog) + 6-BA (6-Benzylaminopurine) 2.534 mg L- 1 + NAA (1-Naphthaleneacetic acid) 0.1 mg L-1, achieving a bud induction rate of 93.3 %. (2) The ideal rooting medium consists of 0.66 MS + IBA (Indole-3-butyric acid) 2.0 mg L- 1 + NAA 0.09 mg L-1. Upon optimizing the culture system, the bud induction rate during the primary culture stage rose to 93.3 %, and the rooting rate during the rooting stage reached 66.7 %. In conclusion, the optimization of culture media using a multifactorial approach proved more effective than the traditional twofactor method, and more accurately optimizes the media of different stages, which provides valuable insights for improving the Bougainvillea tissue culture system.
Keyword ：
Bougainvillea Bougainvillea Response surface analysis Response surface analysis Tissue culture Tissue culture
Cite：
Copy from the list or Export to your reference management。
| GB/T 7714 | Li, Yu , Lin, Wei , Huang, Jing et al. Optimization of Bougainvillea tissue culture system by response surface methodology [J]. | INDUSTRIAL CROPS AND PRODUCTS , 2025 , 226 . |
| MLA | Li, Yu et al. "Optimization of Bougainvillea tissue culture system by response surface methodology" . | INDUSTRIAL CROPS AND PRODUCTS 226 (2025) . |
| APA | Li, Yu , Lin, Wei , Huang, Jing , Ding, Jintao , Li, Jing , He, Bizhu et al. Optimization of Bougainvillea tissue culture system by response surface methodology . | INDUSTRIAL CROPS AND PRODUCTS , 2025 , 226 . |
| Export to | NoteExpress RIS BibTex |
Version ：
Abstract ：
【目的】双组分系统VfmH/VfmI与Dickeya属病原菌的致病因子和致病过程有关。研究D.fangzhongdai Onc5菌株vfmH和vfmI基因的克隆、原核表达及蛋白纯化，为明确VfmH/VfmI的调控机理提供依据。【方法】采用RT-PCR方法扩增D.fangzhongdai Onc5菌株，获取vfmH和vfmI基因开放阅读框（ORF）。随后将目的基因克隆至原核表达质粒pET-32a,构建重组质粒pET-32a-VfmH和pET-32a-VfmI,经异丙基-β-D-硫代半乳糖苷（IPTG）诱导表达后，利用SDS-PAGE和Western blot鉴定重组蛋白，并利用Ni
Keyword ：
vfmH基因 vfmH基因 vfmI基因 vfmI基因 原核表达 原核表达 双组分系统 双组分系统 狄克氏菌 狄克氏菌 软腐病 软腐病
Cite：
Copy from the list or Export to your reference management。
| GB/T 7714 | 王文婷 , 张晨燕 , 赵可冉 et al. 软腐病菌Dickeya fangzhongdai Onc5菌株vfmH和vfmI基因的克隆、原核表达及蛋白纯化 [J]. | 福建农林大学学报(自然科学版) , 2025 , 54 (04) : 447-454 . |
| MLA | 王文婷 et al. "软腐病菌Dickeya fangzhongdai Onc5菌株vfmH和vfmI基因的克隆、原核表达及蛋白纯化" . | 福建农林大学学报(自然科学版) 54 . 04 (2025) : 447-454 . |
| APA | 王文婷 , 张晨燕 , 赵可冉 , 丁建华 , 荆恩荣 , 杨超 . 软腐病菌Dickeya fangzhongdai Onc5菌株vfmH和vfmI基因的克隆、原核表达及蛋白纯化 . | 福建农林大学学报(自然科学版) , 2025 , 54 (04) , 447-454 . |
| Export to | NoteExpress RIS BibTex |
Version ：
Abstract ：
Methyleugenol, a bioactive compound in the phenylpropene family, undergoes its final and crucial biosynthetic transformation when eugenol O-methyltransferase (EOMT) converts eugenol into methyleugenol. While Melaleuca bracteata F. Muell essential oil is particularly rich in methyleugenol, it contains only trace amounts of its precursor, eugenol. This suggests that the EOMT enzyme in M. bracteata is highly efficient, although it has not yet been characterized. In this study, we isolated and identified an EOMT gene from M. bracteata, termed MbEOMT1, which is primarily expressed in the flowers and leaves and is inducible by methyl jasmonate (MeJA). Subcellular localization of MbEOMT1 in the cytoplasm was detected. Through transient overexpression experiments, we found that MbEOMT1 significantly elevates the concentration of methyleugenol in M. bracteata leaves. Conversely, silencing of MbEOMT1 via virus-induced gene silencing led to a marked reduction in methyleugenol levels. Our in vitro enzymatic assays further confirmed that MbEOMT1 specifically catalyzes the methylation of eugenol. Collectively, these findings establish that the MbEOMT1 gene is critical for methyleugenol biosynthesis in M. bracteata. This study enriches the understanding of phenylpropene biosynthesis and suggests that MbEOMT1 could serve as a valuable catalyst for generating bioactive compounds in the future.
Keyword ：
fragrance fragrance methylation methylation O-methyltransferases (OMTs) O-methyltransferases (OMTs) phenylpropene phenylpropene shikimate pathway shikimate pathway synthesis synthesis
Cite：
Copy from the list or Export to your reference management。
| GB/T 7714 | Yang, Chao , Lin, Yongsheng , Xiang, Xuwen et al. MbEOMT1 regulates methyleugenol biosynthesis in Melaleuca bracteata F. Muell [J]. | TREE PHYSIOLOGY , 2024 , 44 (4) . |
| MLA | Yang, Chao et al. "MbEOMT1 regulates methyleugenol biosynthesis in Melaleuca bracteata F. Muell" . | TREE PHYSIOLOGY 44 . 4 (2024) . |
| APA | Yang, Chao , Lin, Yongsheng , Xiang, Xuwen , Shao, Dandan , Qiu, Ziwen , Li, Yongyu et al. MbEOMT1 regulates methyleugenol biosynthesis in Melaleuca bracteata F. Muell . | TREE PHYSIOLOGY , 2024 , 44 (4) . |
| Export to | NoteExpress RIS BibTex |
Version ：
Abstract ：
在中华民族源远流长、博大精深的传统文化体系中，儒家文化占据着重要地位，而朱熹作为宋代儒家大家的代表，对儒家文化进行了深层次分析和解读，因此儒家文化是朱子学说最关键的理论基础。朱子对儒家经典《大学》的解读和注释，积极推动着儒家文化的传承和发展，通过对朱子经典解释著作的拜读，能够窥见朱子一生坚持儒家立场，解读儒家人物思想，高扬儒家传统的根本原因。故而，以朱子对儒家经典著作《大学》的解释为例，客观分析朱子对儒家文化的见解以及立场，对于儒家文化以及朱子学说的发展具有深远意义。
Keyword ：
为己之学 为己之学 儒家立场 儒家立场 朱子 朱子 经典解释 经典解释
Cite：
Copy from the list or Export to your reference management。
| GB/T 7714 | 杨超 . 朱子解释《大学》时的儒家立场探赜——基于“为己之学”视角 [J]. | 中原文学 , 2024 , 3 (23) : 42-44 . |
| MLA | 杨超 . "朱子解释《大学》时的儒家立场探赜——基于“为己之学”视角" . | 中原文学 3 . 23 (2024) : 42-44 . |
| APA | 杨超 . 朱子解释《大学》时的儒家立场探赜——基于“为己之学”视角 . | 中原文学 , 2024 , 3 (23) , 42-44 . |
| Export to | NoteExpress RIS BibTex |
Version ：
Abstract ：
Melaleuca bracteata F. Muell. is among the most significant aromatic plants belonging to the Myrtaceae family, and its essential oil has various biological activities. However, the key enzymes involved in essential oil biosynthesis and their expression patterns are not clearly understood. Appropriate reference genes are required for quantitative real-time polymerase chain reaction (qRT-PCR), sensitive for gene expression studies, to obtain reliable results. In this study, 14 candidate genes, namely ACT, 18S, & alpha;-TUB, fl-TUB, GAPDH, EF1-& alpha;, UBQ, UBC, CYP, EIF-4A, DNAJ, PP2A, TIP41, and UBX, were selected from the M. bracteata transcriptome datasets for analysis under abiotic stresses and hormonal stimuli. The stability of these genes was analyzed using three widely used software programs (geNorm, NormFinder, and BestKeeper). Their results were integrated into a compre-hensive ranking by geometric mean. According to the study results, TIP41 and CYP were the most stable genes for abiotic stresses, while TIP41 and UBQ were the most stable genes for hormonal stimulation. Further, TIP41 and CYP were the most stable reference genes across all tested samples, whereas ACT was the least stable. Based on the expression of two target genes (COMT1 and COMT2, which are involved in methyleugenol biosynthesis), the suitability of the selected reference genes for qRT-PCR normalization was further verified. The study results could offer a foundation for gene expression analysis and functional studies in M. bracteata and other Melaleuca species.
Keyword ：
Abiotic stresses Abiotic stresses Gene expression Gene expression Hormonal stimuli Hormonal stimuli Melaleuca bracteata F Melaleuca bracteata F Muell Muell qRT-PCR qRT-PCR Reference gene Reference gene
Cite：
Copy from the list or Export to your reference management。
| GB/T 7714 | Yang, Chao , Lin, Yongsheng , Qiu, Ziwen et al. Reference gene selection for qRT-PCR normalization of gene expression analysis in Melaleuca bracteata F. Muell. under abiotic stresses and hormonal stimuli [J]. | SCIENTIA HORTICULTURAE , 2023 , 319 . |
| MLA | Yang, Chao et al. "Reference gene selection for qRT-PCR normalization of gene expression analysis in Melaleuca bracteata F. Muell. under abiotic stresses and hormonal stimuli" . | SCIENTIA HORTICULTURAE 319 (2023) . |
| APA | Yang, Chao , Lin, Yongsheng , Qiu, Ziwen , Xiang, Xuwen , Shao, Dandan , Li, Yongyu et al. Reference gene selection for qRT-PCR normalization of gene expression analysis in Melaleuca bracteata F. Muell. under abiotic stresses and hormonal stimuli . | SCIENTIA HORTICULTURAE , 2023 , 319 . |
| Export to | NoteExpress RIS BibTex |
Version ：
Abstract ：
Many aromatic plant volatile compounds contain methyleugenol, which is an attractant for insect pollination and has antibacterial, antioxidant, and other properties. The essential oil of Melaleuca bracteata leaves contains 90.46% methyleugenol, which is an ideal material for studying the biosynthetic pathway of methyleugenol. Eugenol synthase (EGS) is one of the key enzymes involved in the synthesis of methyleugenol. We recently reported two eugenol synthase genes (MbEGS1 and MbEGS2) present in M. bracteata, where MbEGS1 and MbEGS2 were mainly expressed in flowers, followed by leaves, and had the lowest expression levels in stems. In this study, the functions of MbEGS1 and MbEGS2 in the biosynthesis of methyleugenol were investigated using transient gene expression technology and virus-induced gene silencing (VIGS) technology in M. bracteata. Here, in the MbEGSs genes overexpression group, the transcription levels of the MbEGS1 gene and MbEGS2 gene were increased 13.46 times and 12.47 times, respectively, while the methyleugenol levels increased 18.68% and 16.48%. We further verified the function of the MbEGSs genes by using VIGS, as the transcript levels of the MbEGS1 and MbEGS2 genes were downregulated by 79.48% and 90.35%, respectively, and the methyleugenol content in M. bracteata decreased by 28.04% and 19.45%, respectively. The results indicated that the MbEGS1 and MbEGS2 genes were involved in the biosynthesis of methyleugenol, and the transcript levels of the MbEGS1 and MbEGS2 genes correlated with the methyleugenol content in M. bracteata.
Keyword ：
biosynthesis biosynthesis eugenol synthase eugenol synthase Melaleuca bracteata Melaleuca bracteata methyleugenol methyleugenol transient overexpression transient overexpression VIGS VIGS
Cite：
Copy from the list or Export to your reference management。
| GB/T 7714 | Lin, Yongsheng , Qiu, Ziwen , Lin, Xiaojie et al. The Role of MbEGS1 and MbEGS2 in Methyleugenol Biosynthesis by Melaleuca bracteata [J]. | PLANTS-BASEL , 2023 , 12 (5) . |
| MLA | Lin, Yongsheng et al. "The Role of MbEGS1 and MbEGS2 in Methyleugenol Biosynthesis by Melaleuca bracteata" . | PLANTS-BASEL 12 . 5 (2023) . |
| APA | Lin, Yongsheng , Qiu, Ziwen , Lin, Xiaojie , Wu, Yingxiang , Niu, Xianqian , Yin, Guanwen et al. The Role of MbEGS1 and MbEGS2 in Methyleugenol Biosynthesis by Melaleuca bracteata . | PLANTS-BASEL , 2023 , 12 (5) . |
| Export to | NoteExpress RIS BibTex |
Version ：
Abstract ：
Quorum sensing (QS) is a cell-to-cell communication in bacteria that couples gene expression through the accumulation of signaling molecules, which finally induce the production of several virulence factors and modulate bacterial behaviors. Plants have evolved an array of quorum sensing inhibitors (QSIs) to inhibit the pathogens, of which aromatic compounds are widely recognized. The essential oil of Melaleuca bracteata was found to exhibit anti-quorum sensing activity, and its principal bioactive component, methyleugenol (ME), had been isolated in our previous study. Here, ME interfered effectively with the QS-regulated processes of toxin secretion in Chomobacterium violaceum ATCC31532, resulting in strong inhibition of QS genes, cviR, cviI, vioA-E, hmsHNR, lasA-B, pilE1-3, and hcnABC, leading to impaired virulence, including violacein production, biofilm biomass, and swarming motility. The accumulation of the signal molecule (N-hexanoyl-DL-homoserine lactone, C6-HSL) in C. violaceum declined upon treatment with ME, suggesting an inhibition effect on the C6-HSL production, and the ME was also capable of degrading the C6-HSL in vitro assay. Molecular docking technique and the consumption change of exogenous C6-HSL in C. violaceum CV026 revealed the anti-QS mechanism of ME consisted of inhibition of C6-HSL production, potentially via interaction with CviR and/or CviI protein. Collectively, the isolated ME, the principal active components of M. bracteata EO, exhibited a wide range of inhibition processes targeting C. violaceum QS system, which supports the potential anti-pathogenic use of M. bracteata EO and ME for treatment of pathogen contamination caused by bacterial pathogens.
Keyword ：
Chomobacterium violaceum ATCC31532 Chomobacterium violaceum ATCC31532 Melaleuca bracteata EO Melaleuca bracteata EO methyleugenol methyleugenol quorum sensing inhibitor (QSI) quorum sensing inhibitor (QSI) signal molecule (C6-HSL) signal molecule (C6-HSL) virulence factors virulence factors
Cite：
Copy from the list or Export to your reference management。
| GB/T 7714 | Wang, Wenting , Lin, Xiaojie , Yang, Huixiang et al. Anti-quorum sensing evaluation of methyleugenol, the principal bioactive component, from the Melaleuca bracteata leaf oil [J]. | FRONTIERS IN MICROBIOLOGY , 2022 , 13 . |
| MLA | Wang, Wenting et al. "Anti-quorum sensing evaluation of methyleugenol, the principal bioactive component, from the Melaleuca bracteata leaf oil" . | FRONTIERS IN MICROBIOLOGY 13 (2022) . |
| APA | Wang, Wenting , Lin, Xiaojie , Yang, Huixiang , Huang, Xiaoqin , Pan, Lei , Wu, Shaohua et al. Anti-quorum sensing evaluation of methyleugenol, the principal bioactive component, from the Melaleuca bracteata leaf oil . | FRONTIERS IN MICROBIOLOGY , 2022 , 13 . |
| Export to | NoteExpress RIS BibTex |
Version ：
Abstract ：
The mitogen-activated protein kinase (MAPK) cascade consisting of three types of reversibly major signal transduction module (MAPKKK, MAPKK, and MAPK) is distributed in eukaryotes. MAPK cascades participate in various aspects of plant development, including hormone responses, cell division and plant dormancy. Pear is one of the most economically important species worldwide, and its yield is directly affected by dormancy. In this study, genome-wide identification of MAPKK and MAPKKK gene family members in Pyrus x bretschneideri and transcriptional expression analysis of MAPK cascades during pear dormancy were performed. We identified 8 MAPKKs (PbrMKKs) and 100 MAPKKKs (PbrMAPKKKs) in Pyrus using recent genomic information. PbrMAPKKs were classified into four subgroups based on phylogenetic analysis, whereas PbrMAPKKKs were grouped into 3 subfamilies (MEKK, Raf, and ZIK). Most PbrMAPKKKs and PbrMAPKKs in the same subfamily had similar gene structures and conserved motifs. The genes were found on all 17 chromosomes. The comprehensive transcriptome analysis and quantitative real-time polymerase chain reaction (qRT-PCR) results showed that numerous MAPK cascade genes participated in pear bud dormancy. The interaction network and co-expression analyses indicated the crucial roles of the MAPK member-mediated network in pear bud dormancy. Overall, this study advances our understanding of the intricate transcriptional control of MAPKKK-MAPKK-MAPK genes and provides useful information on the functions of dormancy in perennial fruit trees.
Keyword ：
bioinformatics analysis bioinformatics analysis bud dormancy bud dormancy gene family gene family MAPK member-mediated genes MAPK member-mediated genes Pyrus Pyrus
Cite：
Copy from the list or Export to your reference management。
| GB/T 7714 | Liang, Qin , Lin, Xiaojie , Liu, Jinhang et al. Genome-Wide Identification of MAPKK and MAPKKK Gene Family Members and Transcriptional Profiling Analysis during Bud Dormancy in Pear (Pyrus x bretschneideri) [J]. | PLANTS-BASEL , 2022 , 11 (13) . |
| MLA | Liang, Qin et al. "Genome-Wide Identification of MAPKK and MAPKKK Gene Family Members and Transcriptional Profiling Analysis during Bud Dormancy in Pear (Pyrus x bretschneideri)" . | PLANTS-BASEL 11 . 13 (2022) . |
| APA | Liang, Qin , Lin, Xiaojie , Liu, Jinhang , Feng, Yu , Niu, Xianqian , Wang, Chao et al. Genome-Wide Identification of MAPKK and MAPKKK Gene Family Members and Transcriptional Profiling Analysis during Bud Dormancy in Pear (Pyrus x bretschneideri) . | PLANTS-BASEL , 2022 , 11 (13) . |
| Export to | NoteExpress RIS BibTex |
Version ：
Abstract ：
Many aromatic plant volatile compounds contain methyleugenol, which is an attractant for insect pollination and has antibacterial, antioxidant, and other properties. The essential oil of Melaleuca bracteata leaves contains 90.46% methyleugenol, which is an ideal material for studying the biosynthetic pathway of methyleugenol, where eugenol synthase (EGS) is one of the key enzymes involved in the synthesis of methyleugenol. We recently reported two eugenol synthase genes (MbEGS1 and MbEGS2) present in M. bracteata, where MbEGS1 and MbEGS2 were mainly expressed in flowers, followed by leaves, and had the lowest expression levels in stems. In this study, the functions of MbEGS1 and MbEGS2 in the biosynthesis of methyleugenol were investigated using transient gene expression technology and virus-induced gene silencing (VIGS) technology in M. bracteata. Here, in the MbEGSs genes overexpression group, the transcription levels of the MbEGS1 gene and MbEGS2 gene were increased 13.46 times and 12.47 times, respectively, while the methyleugenol levels increased 18.68% and 16.48%. We further verified the function of the MbEGSs genes by using VIGS, as the transcript levels of the MbEGS1 and MbEGS2 genes were downregulated by 79.48% and 90.35%, respectively, and the methyleugenol content in M. bracteata decreased by 28.04% and 19.45%, respectively. The results indicated that the MbEGS1 and MbEGS2 genes were involved in the biosynthesis of methyleugenol, and the transcript levels of the MbEGS1 and MbEGS2 genes correlated with the methyleugenol content in M. bracteata. © 2022, The Authors. All rights reserved.
Keyword ：
Biochemistry Biochemistry Biosynthesis Biosynthesis Essential oils Essential oils Plants (botany) Plants (botany) Transcription Transcription Viruses Viruses Volatile organic compounds Volatile organic compounds
Cite：
Copy from the list or Export to your reference management。
| GB/T 7714 | Lin, Yongsheng , Qiu, Ziwen , Lin, Xiaojie et al. The Role of MbEGS1 and MbEGS2 in Methyleugenol Biosynthesis by Melaleuca bracteata [J]. | SSRN , 2022 . |
| MLA | Lin, Yongsheng et al. "The Role of MbEGS1 and MbEGS2 in Methyleugenol Biosynthesis by Melaleuca bracteata" . | SSRN (2022) . |
| APA | Lin, Yongsheng , Qiu, Ziwen , Lin, Xiaojie , Wu, Yingxiang , Niu, Xianqian , Yin, Guanwen et al. The Role of MbEGS1 and MbEGS2 in Methyleugenol Biosynthesis by Melaleuca bracteata . | SSRN , 2022 . |
| Export to | NoteExpress RIS BibTex |
Version ：
Abstract ：
在前期构建的千层金（Melaleucabracteata）转录组数据库中鉴定并克隆得到2个丁香酚合成酶基因MbEGS1和MbEGS2，基因编码区（codingsequences,CDs）序列长度分别为963和972bp，分别编码320和323个氨基酸。序列比对和系统进化树分析表明，MbEGS1、Mb EGS2与仙女扇（Clarkia breweri）CbIGS1蛋白序列相似性最高，达到65.92%和63.89%。qRT-PCR分析表明，二者在千层金的花、叶片和茎中均有表达，其中在花中表达量最高，叶中次之，茎中最低，与不同组织中丁香酚含量的变化类似，基因表达量与丁香酚含量呈显著正相关；MbEGS1在千层金的根中有表达，且表达量高于茎段，但低于叶片，而MbEGS2在根中不表达。采用不同浓度MeJA处理千层金发现，MeJA能够诱导MbEGS1和MbEGS2表达和丁香酚合成，0.1 mmol·L-1 MeJA诱导效果最显著，其转录水平与丁香酚含量呈正相关。亚细胞定位结果表明，Mb EGS1主要定位于细胞质膜和细胞核中，Mb EGS2主要定位于细胞质膜中。在森林草莓中异位表达MbEGS1和MbEGS2的结果表明，过表达MbEGS1和MbEGS2促进了丁香酚含量的增加。以上结果表明，二者均参与了丁香酚的生物合成。
Keyword ：
丁香酚合成酶 丁香酚合成酶 功能鉴定 功能鉴定 千层金 千层金 基因克隆 基因克隆 表达分析 表达分析
Cite：
Copy from the list or Export to your reference management。
| GB/T 7714 | 邱子文 , 刘林敏 , 林永盛 et al. 千层金MbEGS基因的克隆与功能分析 [J]. | 园艺学报 , 2022 , 49 (08) : 1747-1760 . |
| MLA | 邱子文 et al. "千层金MbEGS基因的克隆与功能分析" . | 园艺学报 49 . 08 (2022) : 1747-1760 . |
| APA | 邱子文 , 刘林敏 , 林永盛 , 林晓洁 , 李永裕 , 吴少华 et al. 千层金MbEGS基因的克隆与功能分析 . | 园艺学报 , 2022 , 49 (08) , 1747-1760 . |
| Export to | NoteExpress RIS BibTex |
Version ：
Export
| Results： |
Selected to |
| Format： |
