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学者姓名：卓孝康
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Abstract ：
Chinese Cymbidium are prized for their ornamental beauty, ecological significance, and economic value. However, genomic resources crucial for breeding studies within this genus remain scarce, which has hindered the identification of key genes controlling economically important traits and posed challenges for conservation efforts. We performed a comprehensive identification of whole-genome simple sequence repeats (SSRs) and single-nucleotide polymorphism (SNP) markers using the restriction-site associated DNA sequencing (RADseq) on C. goeringii and C. faberi. A total of 49,640 SSR loci were identified across both species, with an average density of 12.7 SSRs/Mb. Among these, 17,637 SSRs were common to both C. goeringii and C. faber, while 17,676 and 14,329 SSRs were uniquely identified in C. goeringii and C. faberi, respectively. Additionally, we identified 405,416 SNPs and 26,870 InDels, with average densities of 105.2/Mb and 6.5/Mb. Furthermore, we validated two SSRs (located at Chr01:78857480-78860461 and Chr01:93382182-93384869) and developed an efficient method for identifying hybrids among the progeny resulting from crosses between C. goeringii and C. faberi. We also validated two SNP markers that showed a close association with the petal and lip length using Sanger sequencing. Our findings revealed that the Chr01_99657375 SNP achieved 73% predictive accuracy for identifying long-petal/lip phenotypes. The results are expected to greatly benefit marker-assisted breeding efforts in Cymbidium orchids and lay a solid foundation for the molecular breeding process of improving flower shape traits in orchid plants.
Keyword ：
Cymbidium orchids Cymbidium orchids flower shape traits flower shape traits molecular marker molecular marker RAD-seq RAD-seq
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| GB/T 7714 | Cheng, Mengya , Liu, Yingqi , Jin, Chentai et al. Genome-Wide Identification of SNP and SSR Markers from Cymbidium goeringii and C. faberi for Their Potential Application in Breeding [J]. | HORTICULTURAE , 2025 , 11 (6) . |
| MLA | Cheng, Mengya et al. "Genome-Wide Identification of SNP and SSR Markers from Cymbidium goeringii and C. faberi for Their Potential Application in Breeding" . | HORTICULTURAE 11 . 6 (2025) . |
| APA | Cheng, Mengya , Liu, Yingqi , Jin, Chentai , Jiang, Xiao , Chen, Xiuming , Wang, Fei et al. Genome-Wide Identification of SNP and SSR Markers from Cymbidium goeringii and C. faberi for Their Potential Application in Breeding . | HORTICULTURAE , 2025 , 11 (6) . |
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The Coelogyne s.l. is one of the emblematic genera of the Asian orchids, with high horticultural and medicinal values. However, the phylogenetic relationships of the genus inferred from previous studies based on a limited number of DNA markers remain ambiguous. In this study, we newly sequenced and assembled the complete plastomes of seven Coelogyne species: C. bulleyia, C. fimbriata, C. flaccida, C. prolifera, C. tricallosa, C. uncata, and an unknown taxa, Coelogyne sp. The plastomes of Coelogyne exhibited a typical quadripartite structure, varying in length between 157,476 bp and 160,096 bp, accompanied by a GC content spanning from 37.3% to 37.5%. A total of 132 genes were annotated for each plastome, including 86 protein-coding genes, eight rRNA genes, and 38 tRNA genes. Among these, 19 genes underwent duplication within the inverted repeat (IR) regions, and 18 genes exhibited the presence of introns. Additionally, we detected 54 to 69 simple sequence repeats (SSRs) and 30 to 49 long repeats. In terms of codon usage frequency, leucine (Leu) emerged with the highest frequency, while cysteine (Cys) exhibited the lowest occurrence. Furthermore, eight hypervariable regions (atpB-rbcL, psbK-psbI, rps8-rpl14, rps16-trnQUUG, psaC-ndhE, ndhF-rpl32, psbB-psbT, and ycf1) were identified. Phylogenetic analyses using complete plastomes and protein-coding genes indicated that Coelogyne s.l. was monophyletic. Moreover, the results robustly supported the division of Coelogyne s.l. into five clades. This study provides a comprehensive analysis of the structural variation and phylogenetic analysis of the Coelogyne s.l. based on plastome data. The findings offer significant insights into the plastid genomic characteristics and the phylogenetic relationships of Coelogyne s.l., contributing to a deeper understanding of its evolutionary history.
Keyword ：
Coelogyne Coelogyne hypervariable region hypervariable region phylogenetic analysis phylogenetic analysis plastid genome plastid genome
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| GB/T 7714 | Lin, Songkun , Li, Ruyi , Tang, Shuling et al. Plastomes of Seven Coelogyne s.l. (Arethuseae, Orchidaceae) Species: Comparative Analysis and Phylogenetic Relationships [J]. | HORTICULTURAE , 2025 , 11 (2) . |
| MLA | Lin, Songkun et al. "Plastomes of Seven Coelogyne s.l. (Arethuseae, Orchidaceae) Species: Comparative Analysis and Phylogenetic Relationships" . | HORTICULTURAE 11 . 2 (2025) . |
| APA | Lin, Songkun , Li, Ruyi , Tang, Shuling , Chen, Yuming , Yan, Yin , Gao, Xuyong et al. Plastomes of Seven Coelogyne s.l. (Arethuseae, Orchidaceae) Species: Comparative Analysis and Phylogenetic Relationships . | HORTICULTURAE , 2025 , 11 (2) . |
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Dendrobium wardianum is a key ornamental plant and a valuable traditional Chinese medicine. This research aimed to find the optimal protocol for in vitro in-ducement of polyploidy in D. wardianum by treating protocorms with colchicine (an antimitotic agent). The experiment consisted of two series of treatments. For the first treatment, the protocorms were subjected to colchicine concentrations of 25, 75, 125, 250, and 500 mu M (weight/volume) for 6, 12, and 24 hours. For the second treatment, protocorms were cultivated in culture medium with colchicine (25, 75, 125, and 250 mu M) for 30 days. A total of 18 polyploids were confirmed by chromosome counts and anatomical parameters. Polyploids had broad, dark green leaves with increased stem lengths compared with those of diploids. The optimal protocol for these two methods consisted of soaking in 250 mu M of colchicine solution for 12 hours, resulting in inductivity of 26%, and cultivating in 75 mu M for 30 days, resulting in a mutation rate of 34%. A comparison of these two protocols showed that the latter one induced more stable polyploids, but that the survival rate was slightly lower. The survival and induced mutation rates of these plants were significantly influenced by the colchicine concentration and exposure time. Higher concentrations for longer periods of time re-sulted in greater mortality rates and longer-lasting side effects. The protocol involving a solid medium and colchicine is worth considering. It will be intriguing to examine this methodology for the induction of stable polyploids of other orchid species.
Keyword ：
colchicine colchicine Dendrobium wardianum Dendrobium wardianum orchids orchids polyploidy induction polyploidy induction
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| GB/T 7714 | Wang, Fei , Zhuo, Xiaokang , Arslan, Muhammad et al. In Vitro Induction of Polyploidy by Colchicine in the Protocorm of the Orchid Dendrobium wardianum Warner [J]. | HORTSCIENCE , 2023 , 58 (11) : 1368-1375 . |
| MLA | Wang, Fei et al. "In Vitro Induction of Polyploidy by Colchicine in the Protocorm of the Orchid Dendrobium wardianum Warner" . | HORTSCIENCE 58 . 11 (2023) : 1368-1375 . |
| APA | Wang, Fei , Zhuo, Xiaokang , Arslan, Muhammad , Ercisli, Sezai , Chen, Jinliao , Liu, Zhongjian et al. In Vitro Induction of Polyploidy by Colchicine in the Protocorm of the Orchid Dendrobium wardianum Warner . | HORTSCIENCE , 2023 , 58 (11) , 1368-1375 . |
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Mulberry is an economically important plant in the sericulture industry and traditional medicine. However, the genetic and evolutionary history of mulberry remains largely unknown. Here, this work presents the chromosome-level genome assembly of Morus atropurpurea (M. atropurpurea), originating from south China. Population genomic analysis using 425 mulberry accessions reveal that cultivated mulberry is classified into two species, M. atropurpurea and M. alba, which may have originated from two different mulberry progenitors and have independent and parallel domestication in north and south China, respectively. Extensive gene flow is revealed between different mulberry populations, contributing to genetic diversity in modern hybrid cultivars. This work also identifies the genetic architecture of the flowering time and leaf size. In addition, the genomic structure and evolution of sex-determining regions are identified. This study significantly advances the understanding of the genetic basis and domestication history of mulberry in the north and south, and provides valuable molecular markers of desirable traits for mulberry breeding.
Keyword ：
domestication domestication evolutionary history evolutionary history flowering time flowering time genome genome mulberry mulberry
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| GB/T 7714 | Dai, Fanwei , Zhuo, Xiaokang , Luo, Guoqing et al. Genomic Resequencing Unravels the Genetic Basis of Domestication, Expansion, and Trait Improvement in Morus Atropurpurea [J]. | ADVANCED SCIENCE , 2023 , 10 (24) . |
| MLA | Dai, Fanwei et al. "Genomic Resequencing Unravels the Genetic Basis of Domestication, Expansion, and Trait Improvement in Morus Atropurpurea" . | ADVANCED SCIENCE 10 . 24 (2023) . |
| APA | Dai, Fanwei , Zhuo, Xiaokang , Luo, Guoqing , Wang, Zhenjiang , Xu, Yujuan , Wang, Dan et al. Genomic Resequencing Unravels the Genetic Basis of Domestication, Expansion, and Trait Improvement in Morus Atropurpurea . | ADVANCED SCIENCE , 2023 , 10 (24) . |
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以毛稔叶片作为外植体,MS为基本培养基,研究不同种类激素的浓度配比对毛稔叶片离体再生的影响。结果表明,黑暗条件与光照12 h·d-1有利于毛稔愈伤组织形成,毛稔叶片愈伤组织最佳诱导培养基为MS+0.5 mg·L-16-BA+2.0 mg·L-12,4-D+0.1 mg·L-1NAA,光照12 h·d-1诱导40 d,愈伤诱导率为96%;叶片形成的愈伤组织分化率极低,分化率随6-BA浓度升高而升高,愈伤组织分化最适培养基为MS+0.1 mg·L-1NAA+2.0 mg·L-16-BA,分化率为10%;最适生根培养基为1/2 MS+0.1 mg·L-1NAA,生根率为88.89%。
Keyword ：
再生植株 再生植株 愈伤组织 愈伤组织 毛稔 毛稔 离体培养 离体培养
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| GB/T 7714 | 唐淑玲 , 徐江宇 , 江鸣涛 et al. 毛稔叶片离体培养及植株再生研究 [J]. | 森林与环境学报 , 2016 , 36 (01) : 67-72 . |
| MLA | 唐淑玲 et al. "毛稔叶片离体培养及植株再生研究" . | 森林与环境学报 36 . 01 (2016) : 67-72 . |
| APA | 唐淑玲 , 徐江宇 , 江鸣涛 , 林仙淋 , 卓孝康 , 彭东辉 . 毛稔叶片离体培养及植株再生研究 . | 森林与环境学报 , 2016 , 36 (01) , 67-72 . |
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为筛选出大苞鞘石斛各生长阶段最适培养基配方,并建立大苞鞘石斛快繁体系,以大苞鞘石斛种胚为材料,以1/2MS为基本培养基,通过正交试验研究不同种类激素和添加物的浓度配比对种胚萌发和植株再生的影响。结果表明,大苞鞘石斛种胚萌发和原球茎膨大的最适培养基为1/2 MS+IBA 1.0 mg·L-1+6-BA 2.0 mg·L-1+NAA 2.0 mg·L-1+香蕉泥50 g·L-1,萌发率达91.29%;叶芽分化的最适培养基为1/2MS+IBA 0.5 mg·L-1+6-BA 1.0 mg·L-1+NAA 0.5 mg·L-1+香蕉泥50 g·L-1;干物质积累的最适培养基为1/2 MS+IBA 2.0 mg·L-1+6-BA 2.0 mg·L-1+NAA 0.5 mg·L-1+香蕉泥200 g·L-1;丛生芽增殖的最佳培养基为1/2 MS+IBA 0.5 mg·L-1+6-BA 1.0 mg·L-1+NAA 0.5 mg·L-1+香蕉泥100 g·L-1,平均增殖系数达6.33;丛生芽生根培养的最佳培养基为1/2 MS+IBA 0.5 mg·L-1+6-BA 3.0 mg·L-1+NAA0.2 mg·L-1+香蕉泥50 g·L-1+土豆泥50 g·L-1。
Keyword ：
增殖 增殖 大苞鞘石斛 大苞鞘石斛 生根 生根 胚培养 胚培养
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| GB/T 7714 | 卓孝康 , 兰思仁 , 彭东辉 et al. 大苞鞘石斛胚组织培养及植株再生研究 [J]. | 福建林学院学报 , 2014 , 34 (04) : 289-296 . |
| MLA | 卓孝康 et al. "大苞鞘石斛胚组织培养及植株再生研究" . | 福建林学院学报 34 . 04 (2014) : 289-296 . |
| APA | 卓孝康 , 兰思仁 , 彭东辉 , 吴沙沙 , 江鸣涛 , 张林瀛 . 大苞鞘石斛胚组织培养及植株再生研究 . | 福建林学院学报 , 2014 , 34 (04) , 289-296 . |
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以野牡丹(Melastoma candidum)花粉为材料,利用扫描电镜观察其形态结构,筛选花粉活力测定的最佳方法,探讨光照及不同贮藏条件对花粉萌发的影响。结果表明:野牡丹异型雄蕊花粉的形态无显著差异,花粉粒赤道观为长椭圆形,极面观为三裂圆形,极轴P为33.04(±1.37)μm,赤道轴E为15.05(±0.88)μm;与TTC染色法相比,离体培养基法测定花粉生活力更可靠;紫红色雄蕊和黄色雄蕊上的花粉生活力无显著差异,但二者萌发最适培养基存在差异,适合紫红色雄蕊的最佳萌发培养基为蔗糖50 mg/L+硼酸5 mg/L+CaCl25 mg/L,适合黄色雄蕊的培养基为蔗糖50 mg/L+硼酸10 mg/L+CaCl220 mg/L;光照对花粉萌发无显著影响;不同低温条件贮藏过程中花粉均出现短暂休眠现象,贮藏温度以-7℃和-23℃优于-4℃,贮藏60 d时仍保持较高花粉活力。
Keyword ：
形态 形态 生活力 生活力 花粉 花粉 萌发 萌发 贮藏 贮藏 野牡丹 野牡丹
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| GB/T 7714 | 江鸣涛 , 吴沙沙 , 卓孝康 et al. 野牡丹花粉生活力与贮藏特性研究 [J]. | 广东农业科学 , 2014 , 41 (17) : 42-46 . |
| MLA | 江鸣涛 et al. "野牡丹花粉生活力与贮藏特性研究" . | 广东农业科学 41 . 17 (2014) : 42-46 . |
| APA | 江鸣涛 , 吴沙沙 , 卓孝康 , 唐淑玲 , 李淑娴 , 彭东辉 . 野牡丹花粉生活力与贮藏特性研究 . | 广东农业科学 , 2014 , 41 (17) , 42-46 . |
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以金钗石斛♀×细茎石斛♂杂交F1代种子为材料,探索两种石斛杂交后代的快繁技术和诱导试管开花。结果表明,适于种子萌发的培养基为1/2MS+6-BA 1.0 mg·L-1+NAA 2.0 mg·L-1+IBA 2.0 mg·L-1+香蕉100 g·L-1;继代增殖培养基为1/2MS+6-BA 0.2 mg·L-1+NAA 1.0 mg·L-1+香蕉100 g·L-1,增殖系数达4~5倍;不定芽诱导培养基为1/2MS+6-BA 2.0 mg·L-1+NAA 0.2 mg·L-1+蛋白胨2 g·L-1,诱导率达93.3%,诱导系数达3.7;生根培养基为TH+6-BA 0.5 mg·L-1+NAA 1.0 mg·L-1,生根率可达91.7%,根数3~5条;花芽诱导培养基为6-BA2.0 mg·L-1+NAA 0.5 mg·L-1+IBA 0.5 mg·L-1,花芽诱导率达8%。
Keyword ：
杂交 杂交 石斛 石斛 离体快繁 离体快繁 试管开花 试管开花
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| GB/T 7714 | 卓孝康 , 陈燕琼 , 李淑娴 et al. 金钗石斛♀×细茎石斛♂杂交F_1代的离体快繁与试管开花(简报) [J]. | 亚热带植物科学 , 2014 , 43 (04) : 327-328 . |
| MLA | 卓孝康 et al. "金钗石斛♀×细茎石斛♂杂交F_1代的离体快繁与试管开花(简报)" . | 亚热带植物科学 43 . 04 (2014) : 327-328 . |
| APA | 卓孝康 , 陈燕琼 , 李淑娴 , 漆子钰 , 彭东辉 , 兰思仁 . 金钗石斛♀×细茎石斛♂杂交F_1代的离体快繁与试管开花(简报) . | 亚热带植物科学 , 2014 , 43 (04) , 327-328 . |
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以台湾独蒜兰(Pleione formosana)种胚为材料,研究不同基础培养基、不同种类激素和添加物的浓度配比对种胚萌发和植株再生的影响。结果表明:台湾独蒜兰种胚萌发的最佳配方为MS+NAA0.5mg·L~(-1)+蛋白胨2%,萌发率最高,达88.20%,种苗生长健壮;壮苗培养的配方为TH+NAA 0.5mg·L~(-1)+6-BA1.0mg·L~(-1)+蛋白胨2%;丛生芽增殖和假鳞茎膨大的最佳培养基为MS+6-BA 0.2mg·L~(-1)+NAA 1.0mg·L~(-1)+土豆100g·L~(-1),其增殖系数高,达4.17,假鳞茎饱满,直径达0.33cm;生根培养的最佳配方为MS+6-BA 1.0mg·L~(-1)+NAA 1.0mg·L~(-1)+香蕉泥50g·L~(-1)+土豆50g·L~(-1),根诱导率达78%,平均每株根数为4.67条。
Keyword ：
台湾独蒜兰 台湾独蒜兰 增殖 增殖 生根 生根 胚培养 胚培养
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| GB/T 7714 | 卓孝康 , 兰思仁 , 彭东辉 et al. 台湾独蒜兰无菌萌发与植株再生研究 [C] //中国观赏园艺研究进展（2014） . 2014 . |
| MLA | 卓孝康 et al. "台湾独蒜兰无菌萌发与植株再生研究" 中国观赏园艺研究进展（2014） . (2014) . |
| APA | 卓孝康 , 兰思仁 , 彭东辉 , 吴沙沙 , 陈潇 , 江鸣涛 et al. 台湾独蒜兰无菌萌发与植株再生研究 中国观赏园艺研究进展（2014） . (2014) . |
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在倡导"生态文明"建设的大背景下,建设生态型城市公园是社会的热点话题.本文以公园植物配置为切入点,利用生态学方法和科学植物配置原理,以植物配置的基本要求为指导,从树种组成、生物多样性、植物配置、生态与文化四个方面探讨福州市公园植物配置特点.建议公园植物配置以最大限度地发挥生态效益和环境效益为目标,以生态思想和生物多样性为原则,以带状、线状的绿地为廊道,建立城市生态网,走节约型城市绿化道路.
Keyword ：
城市公园 城市公园 植物配置 植物配置 生态理念 生态理念 生物多样性 生物多样性
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| GB/T 7714 | 卓孝康 , 江鸣涛 , 张林瀛 et al. 基于生态理念探讨福州市公园植物配置 [C] //中国林学会森林公园分会2014年年会论文集 . 2014 : 152-157 . |
| MLA | 卓孝康 et al. "基于生态理念探讨福州市公园植物配置" 中国林学会森林公园分会2014年年会论文集 . (2014) : 152-157 . |
| APA | 卓孝康 , 江鸣涛 , 张林瀛 , 彭东辉 , 兰思仁 . 基于生态理念探讨福州市公园植物配置 中国林学会森林公园分会2014年年会论文集 . (2014) : 152-157 . |
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