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学者姓名：李永裕
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本发明涉及属于食品包装材料领域，本发明公开了一种壳聚糖/余甘提取液/纳米银复合膜及其制备方法。本发明的优点在于：相对于传统纳米银复合膜的制备方法，本方法以壳聚糖作为成膜基质，起到一定的固定纳米银作用；本方法绿色、简便、可控，且制备的壳聚糖/纳米银复合膜表现出了良好物理性能，和简单共混纳米银粒子的方法相比或者和其他高分子成膜材料相比，本发明以壳聚糖作为成膜基质，以PEG‑400与甘油为交联剂，相对于其他高分子物质，例如相对于纤维素，几丁质，异丙醇的组合，本发明采用壳聚糖和PEG‑400、甘油的组合，可以显著增加抗拉强度，减少AgNPs加入对成膜后力学性能的影响，减少了抗菌膜的厚度，所制备的复合膜具有优良的力学性能及抗菌性能。
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| GB/T 7714 | 牛先前 , 李永裕 , 杜丽君 et al. 一种壳聚糖/余甘提取液/纳米银复合膜及其制备方法 : CN202510132854.3[P]. | 2025-02-06 . |
| MLA | 牛先前 et al. "一种壳聚糖/余甘提取液/纳米银复合膜及其制备方法" : CN202510132854.3. | 2025-02-06 . |
| APA | 牛先前 , 李永裕 , 杜丽君 , 康开权 , 刘靖媛 , 阮天一 et al. 一种壳聚糖/余甘提取液/纳米银复合膜及其制备方法 : CN202510132854.3. | 2025-02-06 . |
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Methyleugenol, a bioactive compound in the phenylpropene family, undergoes its final and crucial biosynthetic transformation when eugenol O-methyltransferase (EOMT) converts eugenol into methyleugenol. While Melaleuca bracteata F. Muell essential oil is particularly rich in methyleugenol, it contains only trace amounts of its precursor, eugenol. This suggests that the EOMT enzyme in M. bracteata is highly efficient, although it has not yet been characterized. In this study, we isolated and identified an EOMT gene from M. bracteata, termed MbEOMT1, which is primarily expressed in the flowers and leaves and is inducible by methyl jasmonate (MeJA). Subcellular localization of MbEOMT1 in the cytoplasm was detected. Through transient overexpression experiments, we found that MbEOMT1 significantly elevates the concentration of methyleugenol in M. bracteata leaves. Conversely, silencing of MbEOMT1 via virus-induced gene silencing led to a marked reduction in methyleugenol levels. Our in vitro enzymatic assays further confirmed that MbEOMT1 specifically catalyzes the methylation of eugenol. Collectively, these findings establish that the MbEOMT1 gene is critical for methyleugenol biosynthesis in M. bracteata. This study enriches the understanding of phenylpropene biosynthesis and suggests that MbEOMT1 could serve as a valuable catalyst for generating bioactive compounds in the future.
Keyword ：
fragrance fragrance methylation methylation O-methyltransferases (OMTs) O-methyltransferases (OMTs) phenylpropene phenylpropene shikimate pathway shikimate pathway synthesis synthesis
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| GB/T 7714 | Yang, Chao , Lin, Yongsheng , Xiang, Xuwen et al. MbEOMT1 regulates methyleugenol biosynthesis in Melaleuca bracteata F. Muell [J]. | TREE PHYSIOLOGY , 2024 , 44 (4) . |
| MLA | Yang, Chao et al. "MbEOMT1 regulates methyleugenol biosynthesis in Melaleuca bracteata F. Muell" . | TREE PHYSIOLOGY 44 . 4 (2024) . |
| APA | Yang, Chao , Lin, Yongsheng , Xiang, Xuwen , Shao, Dandan , Qiu, Ziwen , Li, Yongyu et al. MbEOMT1 regulates methyleugenol biosynthesis in Melaleuca bracteata F. Muell . | TREE PHYSIOLOGY , 2024 , 44 (4) . |
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Methyleugenol is widely found in essential oils from aromatic plants, attracting insects for pollination and possessing antimicrobial and antioxidant features. Methyleugenol content in the essential oil from Melaleuca bracteata leaves is 90.46 %, making it an excellent material for assessing methyleugenol's biosynthetic pathway. Eugenol synthase (EGS) and eugenol-o-methyltransferase (EOMT) represent key genes in methyleugenol's biosynthetic pathway but their respective functions are unclear. In this study, overexpression and virus-induced gene silencing (VIGS) techniques were applied to investigate the major regulatory gene among MbEGS1, MbEGS2, MbEGS3, and MbEGS4 in the biosynthesis pathway of methyleugenol. We also aimed to examine the coordination mechanism between EGS and EOMT in methyleugenol production by M. bracteata. The data revealed MbEGS4 transcription and methyleugenol content were most significantly increased after overexpression of the MbEGS1-4 gene in M. bracteata and Fragaria vesca. Conversely, MbEGS4 transcription and methyleugenol content were most significantly reduced after MbEGS1-4 gene silencing by VIGS in M. bracteata. These results underscore a major regulatory role for the MbEGS4 gene in the biosynthetic pathway of methyleugenol in M. bracteata. Remarkably, upon overexpression and silencing of MbEOMTs, the transcription levels of MbEGSs were notably upregulated, with MbEGS4 showing the highest increase. Collectively, these findings suggested the presence of a specific transcription factor bound to the promoters of MbEGS and MbEOMT, inducing their expression for the synergistic and efficient production of methyleugenol in M. bracteata.
Keyword ：
Biosynthesis Biosynthesis Eugenol synthase gene Eugenol synthase gene Methyleugenol Methyleugenol Phenylpropene Phenylpropene
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| GB/T 7714 | Xiang, Xuwen , Song, Keke , Lin, Yongsheng et al. Mechanistic analysis of the coordination between MbEGS4 and MbEOMT in methyleugenol synthesis by Melaleuca bracteata F. Muell [J]. | INDUSTRIAL CROPS AND PRODUCTS , 2024 , 222 . |
| MLA | Xiang, Xuwen et al. "Mechanistic analysis of the coordination between MbEGS4 and MbEOMT in methyleugenol synthesis by Melaleuca bracteata F. Muell" . | INDUSTRIAL CROPS AND PRODUCTS 222 (2024) . |
| APA | Xiang, Xuwen , Song, Keke , Lin, Yongsheng , You, Jingnan , Xu, Yan , Niu, Xianqian et al. Mechanistic analysis of the coordination between MbEGS4 and MbEOMT in methyleugenol synthesis by Melaleuca bracteata F. Muell . | INDUSTRIAL CROPS AND PRODUCTS , 2024 , 222 . |
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Introduction: Watermelon is an annual vine of the family Cucurbitaceae. Watermelon plants produce a fruit that people love and have important nutritional and economic value. With global warming and deterioration of the ecological environment, abiotic stresses, including drought, have become important factors that impact the yield and quality of watermelon plants. Previous research on watermelon drought resistance has included analyzing homologous genes based on known drought-responsive genes and pathways in other species. Methods: However, identifying key pathways and genes involved in watermelon drought resistance through high-throughput omics methods is particularly important. In this study, RNA-seq and metabolomic analysis were performed on watermelon plants at five time points (0 h, 1 h, 6 h, 12 h and 24 h) before and after drought stress. Results: Transcriptomic analysis revealed 7829 differentially expressed genes (DEGs) at the five time points. The DEGs were grouped into five clusters using the k-means clustering algorithm. The functional category for each cluster was annotated based on the Kyoto Encyclopedia of Genes and Genomes (KEGG) database; different clusters were associated with different time points after stress. A total of 949 metabolites were divided into 10 categories, with lipids and lipid-like molecules accounting for the most metabolites. Differential expression analysis revealed 22 differentially regulated metabolites (DRMs) among the five time points. Through joint analysis of RNA-seq and metabolome data, the 6-h period was identified as the critical period for watermelon drought resistance, and the starch and sucrose metabolism, plant hormone signal transduction and photosynthesis pathways were identified as important regulatory pathways involved in watermelon drought resistance. In addition, 15 candidate genes associated with watermelon drought resistance were identified through joint RNA-seq and metabolome analysis combined with weighted correlation network analysis (WGCNA). Four of these genes encode transcription factors, including bHLH (Cla97C03G068160), MYB (Cla97C01G002440), HSP (Cla97C02G033390) and GRF (Cla97C02G042620), one key gene in the ABA pathway, SnRK2-4 (Cla97C10G186750), and the GP-2 gene (Cla97C05G105810), which is involved in the starch and sucrose metabolism pathway. Discussion: In summary, our study provides a theoretical basis for elucidating the molecular mechanisms underlying drought resistance in watermelon plants and provides new genetic resources for the study of drought resistance in this crop.
Keyword ：
drought drought metabolomic metabolomic RNA-Seq RNA-Seq watermelon watermelon WGCNA WGCNA
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| GB/T 7714 | Chen, Sheng , Zhong, Kaiqin , Li, Yongyu et al. Joint transcriptomic and metabolomic analysis provides new insights into drought resistance in watermelon (Citrullus lanatus) [J]. | FRONTIERS IN PLANT SCIENCE , 2024 , 15 . |
| MLA | Chen, Sheng et al. "Joint transcriptomic and metabolomic analysis provides new insights into drought resistance in watermelon (Citrullus lanatus)" . | FRONTIERS IN PLANT SCIENCE 15 (2024) . |
| APA | Chen, Sheng , Zhong, Kaiqin , Li, Yongyu , Bai, Changhui , Xue, Zhuzheng , Wu, Yufen . Joint transcriptomic and metabolomic analysis provides new insights into drought resistance in watermelon (Citrullus lanatus) . | FRONTIERS IN PLANT SCIENCE , 2024 , 15 . |
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Bud endodormancy represents a pivotal and intricate biological process influenced by both genetic and epigenetic factors, the exact mechanism of which remains elusive. Hydrogen peroxide (H2O2) functions as a signalling molecule in the regulation of dormancy, with peroxidase (POD) playing a crucial role in governing H2O2 levels. Our prior transcriptomic and metabolomic investigations into diverse pear dormancy phases posited that POD predominantly oversees pear bud dormancy. In this study, we utilised qRT-PCR to screen the most significantly expressed gene, Pyrus pyrifolia POD4-like (PpPOD4-like), from seven POD genes. Subsequently, H2O2 test kits, overexpression methods, and subcellular localisation techniques were employed to assess changes in H2O2 content, POD activity, PpPOD4-like expression, and its cellular positioning during pear bud dormancy. Subcellular localisation experiments revealed that PpPOD4-like is situated on the cell membranes. Notably, H2O2 content exhibited a rapid increase during endodormancy and decreased swiftly after ecodormancy. The fluctuation pattern of POD activity aligned with that of H2O2 content. Additionally, PpPOD4-like expression was markedly upregulated, displaying an overall upward trajectory. Our findings indicate that PpPOD4-like modulates H2O2 levels by regulating POD activity, thereby actively participating in the intricate regulation of pear dormancy processes.
Keyword ：
endodormancy endodormancy H2O2 H2O2 overexpression overexpression POD genes POD genes
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| GB/T 7714 | Xiang, Xuwen , Song, Keke , Li, Yinyin et al. Screening and Expression Analysis of POD Gene in POD-H2O2 Pathway on Bud Dormancy of Pear (Pyrus pyrifolia) [J]. | FORESTS , 2024 , 15 (3) . |
| MLA | Xiang, Xuwen et al. "Screening and Expression Analysis of POD Gene in POD-H2O2 Pathway on Bud Dormancy of Pear (Pyrus pyrifolia)" . | FORESTS 15 . 3 (2024) . |
| APA | Xiang, Xuwen , Song, Keke , Li, Yinyin , Zhang, Chenyu , Zhou, Ruiqi , Feng, Yu et al. Screening and Expression Analysis of POD Gene in POD-H2O2 Pathway on Bud Dormancy of Pear (Pyrus pyrifolia) . | FORESTS , 2024 , 15 (3) . |
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研究旨在通过开展福建不同百香果产区果实品质研究，获得福建百香果优势种植区域，为引导果农优势发展提供指导。以福建省不同产地的‘福建百香果1号’和‘福建百香果3号’为材料，使用主成分分析法对百香果品质进行综合评价，通过品质测定分析比较不同产地百香果果实品质的差异。结果表明，不同产地百香果品质之间存在差异，其中单果重、可食率、糖酸比为影响果实品质的主要因素。延平种植的‘福建百香果1号’单果重和可食率最高，且极显著高于新罗。糖酸比最高的为新罗(3.03)，其次为尤溪、延平，古田最低。‘福建百香果3号’单果重最高为惠安(92.87 g)，极显著高于华安、福安、武平；可食率最高为华安(55.26%)，与福安(17.8%)差异达到极显著水平；糖酸比最高为惠安(6.79)，极显著高于福安、华安和武平。‘福建百香果1号’综合得分最高的为延平(0.2194)，最低为古田(0.1405)。‘福建百香果3号’综合得分最高华安、惠安(0.2461)。福建地区百香果果实品质与产区纬度有相关性，而与经度关系不明显；‘福建百香果1号’品质最佳种植区域为25°—26°N，‘福建百香果3号’为25°N左右。在适宜纬度范围内一定海拔高度的区域，不仅有利于提升果实品质，而且可能具有降低百香果高温障碍的效果。
Keyword ：
产地 产地 区域划分 区域划分 果实品质 果实品质 海拔 海拔 百香果 百香果 经纬度 经纬度
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| GB/T 7714 | 牛先前 , 江莉 , 林秋金 et al. 福建百香果果实品质优势区域划分 [J]. | 中国农学通报 , 2023 , 39 (07) : 40-45 . |
| MLA | 牛先前 et al. "福建百香果果实品质优势区域划分" . | 中国农学通报 39 . 07 (2023) : 40-45 . |
| APA | 牛先前 , 江莉 , 林秋金 , 林秀香 , 李永裕 , 施清 . 福建百香果果实品质优势区域划分 . | 中国农学通报 , 2023 , 39 (07) , 40-45 . |
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Melaleuca bracteata F. Muell. is among the most significant aromatic plants belonging to the Myrtaceae family, and its essential oil has various biological activities. However, the key enzymes involved in essential oil biosynthesis and their expression patterns are not clearly understood. Appropriate reference genes are required for quantitative real-time polymerase chain reaction (qRT-PCR), sensitive for gene expression studies, to obtain reliable results. In this study, 14 candidate genes, namely ACT, 18S, & alpha;-TUB, fl-TUB, GAPDH, EF1-& alpha;, UBQ, UBC, CYP, EIF-4A, DNAJ, PP2A, TIP41, and UBX, were selected from the M. bracteata transcriptome datasets for analysis under abiotic stresses and hormonal stimuli. The stability of these genes was analyzed using three widely used software programs (geNorm, NormFinder, and BestKeeper). Their results were integrated into a compre-hensive ranking by geometric mean. According to the study results, TIP41 and CYP were the most stable genes for abiotic stresses, while TIP41 and UBQ were the most stable genes for hormonal stimulation. Further, TIP41 and CYP were the most stable reference genes across all tested samples, whereas ACT was the least stable. Based on the expression of two target genes (COMT1 and COMT2, which are involved in methyleugenol biosynthesis), the suitability of the selected reference genes for qRT-PCR normalization was further verified. The study results could offer a foundation for gene expression analysis and functional studies in M. bracteata and other Melaleuca species.
Keyword ：
Abiotic stresses Abiotic stresses Gene expression Gene expression Hormonal stimuli Hormonal stimuli Melaleuca bracteata F Melaleuca bracteata F Muell Muell qRT-PCR qRT-PCR Reference gene Reference gene
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| GB/T 7714 | Yang, Chao , Lin, Yongsheng , Qiu, Ziwen et al. Reference gene selection for qRT-PCR normalization of gene expression analysis in Melaleuca bracteata F. Muell. under abiotic stresses and hormonal stimuli [J]. | SCIENTIA HORTICULTURAE , 2023 , 319 . |
| MLA | Yang, Chao et al. "Reference gene selection for qRT-PCR normalization of gene expression analysis in Melaleuca bracteata F. Muell. under abiotic stresses and hormonal stimuli" . | SCIENTIA HORTICULTURAE 319 (2023) . |
| APA | Yang, Chao , Lin, Yongsheng , Qiu, Ziwen , Xiang, Xuwen , Shao, Dandan , Li, Yongyu et al. Reference gene selection for qRT-PCR normalization of gene expression analysis in Melaleuca bracteata F. Muell. under abiotic stresses and hormonal stimuli . | SCIENTIA HORTICULTURAE , 2023 , 319 . |
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Many aromatic plant volatile compounds contain methyleugenol, which is an attractant for insect pollination and has antibacterial, antioxidant, and other properties. The essential oil of Melaleuca bracteata leaves contains 90.46% methyleugenol, which is an ideal material for studying the biosynthetic pathway of methyleugenol. Eugenol synthase (EGS) is one of the key enzymes involved in the synthesis of methyleugenol. We recently reported two eugenol synthase genes (MbEGS1 and MbEGS2) present in M. bracteata, where MbEGS1 and MbEGS2 were mainly expressed in flowers, followed by leaves, and had the lowest expression levels in stems. In this study, the functions of MbEGS1 and MbEGS2 in the biosynthesis of methyleugenol were investigated using transient gene expression technology and virus-induced gene silencing (VIGS) technology in M. bracteata. Here, in the MbEGSs genes overexpression group, the transcription levels of the MbEGS1 gene and MbEGS2 gene were increased 13.46 times and 12.47 times, respectively, while the methyleugenol levels increased 18.68% and 16.48%. We further verified the function of the MbEGSs genes by using VIGS, as the transcript levels of the MbEGS1 and MbEGS2 genes were downregulated by 79.48% and 90.35%, respectively, and the methyleugenol content in M. bracteata decreased by 28.04% and 19.45%, respectively. The results indicated that the MbEGS1 and MbEGS2 genes were involved in the biosynthesis of methyleugenol, and the transcript levels of the MbEGS1 and MbEGS2 genes correlated with the methyleugenol content in M. bracteata.
Keyword ：
biosynthesis biosynthesis eugenol synthase eugenol synthase Melaleuca bracteata Melaleuca bracteata methyleugenol methyleugenol transient overexpression transient overexpression VIGS VIGS
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| GB/T 7714 | Lin, Yongsheng , Qiu, Ziwen , Lin, Xiaojie et al. The Role of MbEGS1 and MbEGS2 in Methyleugenol Biosynthesis by Melaleuca bracteata [J]. | PLANTS-BASEL , 2023 , 12 (5) . |
| MLA | Lin, Yongsheng et al. "The Role of MbEGS1 and MbEGS2 in Methyleugenol Biosynthesis by Melaleuca bracteata" . | PLANTS-BASEL 12 . 5 (2023) . |
| APA | Lin, Yongsheng , Qiu, Ziwen , Lin, Xiaojie , Wu, Yingxiang , Niu, Xianqian , Yin, Guanwen et al. The Role of MbEGS1 and MbEGS2 in Methyleugenol Biosynthesis by Melaleuca bracteata . | PLANTS-BASEL , 2023 , 12 (5) . |
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The indole-3-acetic acid (IAA) auxin is an important endogenous hormone that plays a key role in the regulation of plant growth and development. In recent years, with the progression of auxin-related research, the function of the Gretchen Hagen 3 (GH3) gene has become a prominent research topic. However, studies focusing on the characteristics and functions of melon GH3 family genes are still lacking. This study presents a systematic identification of melon GH3 gene family members based on genomic data. The evolution of melon GH3 family genes was systematically analyzed by means of bioinformatics, and the expression patterns of the GH3 family genes in different melon tissues during different fruit developmental stages and with various levels of 1-naphthaleneacetic acid (NAA) induction were analyzed with transcriptomics and RT-qPCR. The melon genome contains 10 GH3 genes distributed across seven chromosomes, and most of these genes are expressed in the plasma membrane. According to evolutionary analysis and the number of GH3 family genes, these genes can be divided into three subgroups, and they have been conserved throughout the evolution of melon. The melon GH3 gene has a wide range of expression patterns across distinct tissue types, with expression generally being higher in flowers and fruit. Through promoter analysis, we found that most cis-acting elements contained light- and IAA-responsive elements. Based on the RNA-seq and RT-qPCR analyses, it can be speculated that CmGH3-5, CmGH3-6 and CmGH3-7 may be involved in the process of melon fruit development. In conclusion, our findings suggest that the GH3 gene family plays an important role in the development of melon fruit. This study provides an important theoretical basis for further research on the function of the GH3 gene family and the molecular mechanism underlying the development of melon fruit.
Keyword ：
evolutionary analysis evolutionary analysis expression analysis expression analysis fruit development fruit development GH3 gene family GH3 gene family melon melon
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| GB/T 7714 | Chen, Sheng , Zhong, Kaiqin , Li, Yongyu et al. Evolutionary Analysis of the Melon (Cucumis melo L.) GH3 Gene Family and Identification of GH3 Genes Related to Fruit Growth and Development [J]. | PLANTS-BASEL , 2023 , 12 (6) . |
| MLA | Chen, Sheng et al. "Evolutionary Analysis of the Melon (Cucumis melo L.) GH3 Gene Family and Identification of GH3 Genes Related to Fruit Growth and Development" . | PLANTS-BASEL 12 . 6 (2023) . |
| APA | Chen, Sheng , Zhong, Kaiqin , Li, Yongyu , Bai, Changhui , Xue, Zhuzheng , Wu, Yufen . Evolutionary Analysis of the Melon (Cucumis melo L.) GH3 Gene Family and Identification of GH3 Genes Related to Fruit Growth and Development . | PLANTS-BASEL , 2023 , 12 (6) . |
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本发明公开了一种基于长余辉纳米探针的植物成像方法，应用的方法包括：制备长余辉纳米探针，本方法使用的纳米探针是表面修饰聚丙烯酸的Zn1.1Ga1.8Ge0.1O4 : 0.5％Cr长余辉纳米材料；将长余辉纳米探针通过水培介质暴露在植物生长环境中，用LED灯照射植物、激发探针后，实现植物整株活体成像。本发明探针具有在激发光源关闭后持续发光的性质，能有效抑制植物组织背景荧光干扰，克服了传统植物成像技术信噪比低的最大缺点。本发明除了能实现植物活体的高灵敏度成像，还可以省去切片、染色等操作；长波长激发光源、近红外发射余辉位于生物窗口，对组织无损伤，可多次重复激发成像。本发明可实现对植物中纳米颗粒分布的高效可视化，在植物纳米毒理学研究领域有巨大的应用前景。
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| GB/T 7714 | 张云 , 俞可欣 , 李永裕 et al. 基于长余辉纳米探针的植物成像方法 : CN202211532798.5[P]. | 2022-12-01 . |
| MLA | 张云 et al. "基于长余辉纳米探针的植物成像方法" : CN202211532798.5. | 2022-12-01 . |
| APA | 张云 , 俞可欣 , 李永裕 , 孙霞 , 史俊朋 . 基于长余辉纳米探针的植物成像方法 : CN202211532798.5. | 2022-12-01 . |
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