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一种从平沙绿僵菌中提取3β-acetoxy-15α,22-dihydroxyhopane的方法 ipsunlight
专利 | 2025-03-18 | CN202510317536.4
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本发明公开了一种从平沙绿僵菌中提取3β‑acetoxy‑15α,22‑dihydroxyhopane的方法,其是先将平沙绿僵菌在PDA培养基中进行活化培养,然后转移到PDB培养基中进行初级培养,再接种到大米培养基中进行次级培养,之后经超声提取结合乙酸乙酯浸提,浓缩,获得粗提物,再将粗提物依次经减压柱、正相硅胶柱进行分离纯化,从而获得3β‑acetoxy‑15α,22‑dihydroxyhopane。本发明首次在平沙绿僵菌中分离获得3β‑acetoxy‑15α,22‑dihydroxyhopane,并证明其可用于茶尺蠖的生物防治。

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GB/T 7714 关夏玉 , 黄文德 , 陈泽轩 et al. 一种从平沙绿僵菌中提取3β-acetoxy-15α,22-dihydroxyhopane的方法 : CN202510317536.4[P]. | 2025-03-18 .
MLA 关夏玉 et al. "一种从平沙绿僵菌中提取3β-acetoxy-15α,22-dihydroxyhopane的方法" : CN202510317536.4. | 2025-03-18 .
APA 关夏玉 , 黄文德 , 陈泽轩 , 林永盛 , 曹伟强 , 熊成杰 et al. 一种从平沙绿僵菌中提取3β-acetoxy-15α,22-dihydroxyhopane的方法 : CN202510317536.4. | 2025-03-18 .
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New entomopathogenic species in the Clavicipitaceae family (Hypocreales, Ascomycota) from the subtropical forests of Fujian, China SCIE
期刊论文 | 2025 , 16 | FRONTIERS IN MICROBIOLOGY
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Introduction Entomopathogenic fungi play a crucial role in the ecological regulation of insect populations and can be exploited as a resource for pest control, sustainable agriculture, and natural products discovery. These fungi and their infected hosts are sometimes highly coveted as part of traditional medicine practices. Here, we sought to examine the biodiversity of entomogenous fungi in subtropical forests of China.Methods Fungal-infected insect specimens were collected from various sites in Fujian Province, China, and purified isolates were obtained through laboratory cultivation and isolation techniques. Molecular characterization of specific target genomic loci was performed on the fungal isolates, and used for phylogenetic analyses using Bayesian inference and maximum likelihood methods to elucidate their taxonomic relationships. Microscopy was used to describe the morphological features of the isolates.Results Through a comprehensive two-year survey of Fujian Province via multilocus molecular phylogenetic analysis targeting the nrSSU, nrLSU, tef1-alpha, rpb1, and rpb2 loci of collected specimens, we identified three novel species within the Clavicipitaceae herein described as: Albacillium fuzhouense sp. nov., Conoideocrella gongyashanensis sp. nov. and Neoaraneomyces wuyishanensis sp. nov., as well as the recently recorded, Metarhizium cicadae. Each new species was also distinguished from its closest relatives by unique morphological characteristics.Discussion These discoveries enrich our understanding of biodiversity within the Clavicipitaceae family and can contribute to the development of new pest control strategies and natural products discovery.

Keyword :

Albacillium Albacillium Clavicipitaceae Clavicipitaceae Conoideocrella Conoideocrella entomopathogenic fungi entomopathogenic fungi Metarhizium Metarhizium Neoaraneomyces Neoaraneomyces new taxa new taxa

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GB/T 7714 Lin, Longbing , Lin, Yongsheng , Keyhani, Nemat O. et al. New entomopathogenic species in the Clavicipitaceae family (Hypocreales, Ascomycota) from the subtropical forests of Fujian, China [J]. | FRONTIERS IN MICROBIOLOGY , 2025 , 16 .
MLA Lin, Longbing et al. "New entomopathogenic species in the Clavicipitaceae family (Hypocreales, Ascomycota) from the subtropical forests of Fujian, China" . | FRONTIERS IN MICROBIOLOGY 16 (2025) .
APA Lin, Longbing , Lin, Yongsheng , Keyhani, Nemat O. , Pu, Huili , Yang, Jiao , Xiong, Chengjie et al. New entomopathogenic species in the Clavicipitaceae family (Hypocreales, Ascomycota) from the subtropical forests of Fujian, China . | FRONTIERS IN MICROBIOLOGY , 2025 , 16 .
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New Species of Diaporthales (Ascomycota) from Diseased Leaves in Fujian Province, China SCIE
期刊论文 | 2025 , 11 (1) | JOURNAL OF FUNGI
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Fungal biota represents important constituents of phyllosphere microorganisms. It is taxonomically highly diverse and influences plant physiology, metabolism and health. Members of the order Diaporthales are distributed worldwide and include devastating plant pathogens as well as endophytes and saprophytes. However, many phyllosphere Diaporthales species remain uncharacterized, with studies examining their diversity needed. Here, we report on the identification of several diaporthalean taxa samples collected from diseased leaves of Cinnamomum camphora (Lauraceae), Castanopsis fordii (Fagaceae) and Schima superba (Theaceae) in Fujian province, China. Based on morphological features coupled to multigene phylogenetic analyses of the internal transcribed spacer (ITS) region, the large subunit of nuclear ribosomal RNA (LSU), the partial beta-tubulin (tub2), histone H3 (his3), DNA-directed RNA polymerase II subunit (rpb2), translation elongation factor 1-alpha (tef1) and calmodulin (cal) genes, three new species of Diaporthales are introduced, namely, Diaporthe wuyishanensis, Gnomoniopsis wuyishanensis and Paratubakia schimae. This study contributes to our understanding on the biodiversity of diaporthalean fungi that are inhabitants of the phyllosphere of trees native to Asia.

Keyword :

Diaporthe Diaporthe multigene phylogeny multigene phylogeny new species new species Paratubakia Paratubakia taxonomy taxonomy

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GB/T 7714 Guan, Xiayu , Mu, Taichang , Keyhani, Nemat O. et al. New Species of Diaporthales (Ascomycota) from Diseased Leaves in Fujian Province, China [J]. | JOURNAL OF FUNGI , 2025 , 11 (1) .
MLA Guan, Xiayu et al. "New Species of Diaporthales (Ascomycota) from Diseased Leaves in Fujian Province, China" . | JOURNAL OF FUNGI 11 . 1 (2025) .
APA Guan, Xiayu , Mu, Taichang , Keyhani, Nemat O. , Shang, Junya , Mao, Yuchen , Yang, Jiao et al. New Species of Diaporthales (Ascomycota) from Diseased Leaves in Fujian Province, China . | JOURNAL OF FUNGI , 2025 , 11 (1) .
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Mitochondrial Genomes from Fungal the Entomopathogenic Moelleriella Genus Reveals Evolutionary History, Intron Dynamics and Phylogeny SCIE
期刊论文 | 2025 , 11 (2) | JOURNAL OF FUNGI
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Members of the genus Moelleriella (Hypocreales, Clavicipitaceae) are insect pathogens with specificity for scale insects and whiteflies. However, no mitochondrial genomes are available for these fungi. Here, we assembled seven mitogenomes from M. zhongdongii, M. libera, M. raciborskii, M. gracilispora, M. oxystoma, Moelleriella sp. CGMCC 3.18909, and Moelleriella sp. CGMCC 3.18913, which varied in size from 40.8 to 95.7 Kb. Synteny and codon usage bias was relatively conserved, with the mitochondrial gene arrangement being completely homologous to the gene order of 21 other species within the Hypocreales. Nevertheless, significant intron polymorphism was observed between Moelleriella species. Evolutionary analyses revealed that all 15 core protein-coding genes had ka/ks < 1, indicating purifying selection pressure. Sequence variation within the mitochondrial ATP synthase F0 subunit 6 (atp6) gene showed the largest genetic distance, with the ATP synthase F0 subunit 9 (atp9) gene showing the smallest. Comparative analyses of mitogenomes revealed that introns were the primary factor contributing to the size variation in Moelleriella and, more broadly, within Hypocreales mitogenomes. Phylogenetic analyses indicated that the seven Moelleriella species examined form a well-supported clade, most closely related to Metarhizium. These data present the first mitogenomes from Moelleriella and further advance research into the taxonomy, origin, evolution, and genomics of Moelleriella.

Keyword :

Clavicipitaceae Clavicipitaceae Hypocreales Hypocreales insect pathogen insect pathogen mitogenome mitogenome Moelleriella Moelleriella phylogeny analysis phylogeny analysis

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GB/T 7714 Xiong, Chengjie , Lin, Yongsheng , Keyhani, Nemat O. et al. Mitochondrial Genomes from Fungal the Entomopathogenic Moelleriella Genus Reveals Evolutionary History, Intron Dynamics and Phylogeny [J]. | JOURNAL OF FUNGI , 2025 , 11 (2) .
MLA Xiong, Chengjie et al. "Mitochondrial Genomes from Fungal the Entomopathogenic Moelleriella Genus Reveals Evolutionary History, Intron Dynamics and Phylogeny" . | JOURNAL OF FUNGI 11 . 2 (2025) .
APA Xiong, Chengjie , Lin, Yongsheng , Keyhani, Nemat O. , Shang, Junya , Mao, Yuchen , Yang, Jiao et al. Mitochondrial Genomes from Fungal the Entomopathogenic Moelleriella Genus Reveals Evolutionary History, Intron Dynamics and Phylogeny . | JOURNAL OF FUNGI , 2025 , 11 (2) .
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Two new species of Ascomycota on bamboo leaves in Fujian, China SCIE
期刊论文 | 2025 , 16 | FRONTIERS IN MICROBIOLOGY
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Introduction Bamboo is the largest member of the family Poaceae, and these fast-growing plants have many beneficial ecological effects. Both mutualistic and pathogenic fungi associate with bamboo, however, only a limited number of such fungal species have been identified, and information concerning the diversity of fungi that parasitizes bamboo leaves is sparse.Methods Fungi were isolated from diseased leaves of bamboo from Fujian Province, China. Nucleotide sequences of four genetic loci were used for taxonomic and phylogenetic reconstruction. In addition, the morphological characteristics of fungal structures as well as growth parameters were characterized.Results We report two new fungal species, one each within the Didymosphaeriaceae and Magnaporthaceae, both diverse genera that include saprophytes, endophytes, and pathogens. Based on combined morphological and phylogenetic analyses, including the nucleotide sequences of the internal transcribed spacer (ITS), the 28S large subunit of ribosomal RNA (LSU), the 18S small subunit of nuclear ribosomal RNA gene (SSU), the large subunit of RNA polymerase I (rpb1), and the translation elongation factor 1-alpha gene (tef1-alpha), the two new species described herein have been named as Bifusisporella magnum sp. nov., and Letendraea bambusae sp. nov. Detailed descriptions and morphological features of the species are provided.Discussion These data identify and describe new species within the Didymosphaeriaceae and Magnaporthaceae, expanding the diversity and distribution of fungi associating with bamboo.

Keyword :

Didymosphaeriaceae Didymosphaeriaceae fungal morphology fungal morphology fungal phylogenetics fungal phylogenetics Magnaporthaceae Magnaporthaceae taxonomy taxonomy

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GB/T 7714 Guan, Xiayu , Zhao, Zhiying , Keyhani, Nemat O. et al. Two new species of Ascomycota on bamboo leaves in Fujian, China [J]. | FRONTIERS IN MICROBIOLOGY , 2025 , 16 .
MLA Guan, Xiayu et al. "Two new species of Ascomycota on bamboo leaves in Fujian, China" . | FRONTIERS IN MICROBIOLOGY 16 (2025) .
APA Guan, Xiayu , Zhao, Zhiying , Keyhani, Nemat O. , Mu, Taichang , Zheng, Minhai , Yang, Lixia et al. Two new species of Ascomycota on bamboo leaves in Fujian, China . | FRONTIERS IN MICROBIOLOGY , 2025 , 16 .
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Three new species of Neopestalotiopsis and Pseudopestalotiopsis (Sporocadaceae, Amphisphaeriales) associated with shrub leaf diseases from Fujian, China SCIE
期刊论文 | 2025 , (119) , 1-28 | MYCOKEYS
WoS CC Cited Count: 3
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Neopestalotiopsis and Pseudopestalotiopsis are classified as pestalotioid fungi, include a diverse range of plant pathogenic, endophytic and saprobic species and are widely distributed in tropical and temperate climates. These fungi are associated with a wide variety of plants worldwide and are exemplified by multi-septate conidia with appendages at both ends. Phytopathogenic members cause various plant diseases, for example, leaf spot, fruit rot, canker, blight and various infections affecting palm, mango, blueberry, tea and other important crops. In this study, six isolates were collected from diseased leaves of Litsea verticillata, Ixora chinensis and an unidentified shrub in Fujian Province, China. Based on morphological characteristics and molecular phylogenetic analyses of combined nucleotide sequences of internal transcribed spacer regions of rDNA (ITS), the partial translation elongation factor 1-alpha gene (tef1) and partial beta-tubulin gene (tub2), three new species Neopestalotiopsis litseae sp. nov., Neo. longqishanensis sp. nov. and Pseudopestalotiopsis zhangzhouensis sp. nov. are described and illustrated herein.

Keyword :

Morphology Morphology multigene phylogeny multigene phylogeny new taxa new taxa pestalotioid fungi pestalotioid fungi

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GB/T 7714 Heng, Zhi-Ang , Mu, Tai-Chang , Keyhani, Nemat O. et al. Three new species of Neopestalotiopsis and Pseudopestalotiopsis (Sporocadaceae, Amphisphaeriales) associated with shrub leaf diseases from Fujian, China [J]. | MYCOKEYS , 2025 , (119) : 1-28 .
MLA Heng, Zhi-Ang et al. "Three new species of Neopestalotiopsis and Pseudopestalotiopsis (Sporocadaceae, Amphisphaeriales) associated with shrub leaf diseases from Fujian, China" . | MYCOKEYS 119 (2025) : 1-28 .
APA Heng, Zhi-Ang , Mu, Tai-Chang , Keyhani, Nemat O. , Yang, Li-Xia , Zheng, Ming-Hai , Lv, Hua-Jun et al. Three new species of Neopestalotiopsis and Pseudopestalotiopsis (Sporocadaceae, Amphisphaeriales) associated with shrub leaf diseases from Fujian, China . | MYCOKEYS , 2025 , (119) , 1-28 .
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Phylogeographic History of Tomato Chlorosis Virus SCIE
期刊论文 | 2025 , 17 (4) | VIRUSES-BASEL
WoS CC Cited Count: 1
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Tomato chlorosis virus (ToCV), first reported in Florida, USA, in 1998, has since emerged in multiple regions worldwide, posing a significant threat to global tomato production. However, its origin, migration patterns, and evolutionary history remain poorly understood. In this study, we used Bayesian phylogeographic analysis of coat protein gene sequences from 155 ToCV isolates to reconstruct its phylogeographic history. Our results show that ToCV evolves at a rate of 6.24 x 10-4 subs/site/year (95% credibility interval: 4.35 x 10-4-8.28 x 10-4), with the most recent common ancestor dating back to 1882. The maximum clade credibility (MCC) tree revealed three major clades, with Clade 1-whose most recent common ancestor dates to approximately 1975-comprising over 90% of the isolates. Although the exact origin of ToCV remains uncertain, we identified five distinct migration pathways: one from Europe to the Americas, one from Europe to South Asia, one from the Middle East to East Asia, one from East Asia to mainland China, and one from mainland China to Europe. These findings underscore the complex global spread of ToCV and suggest that multiple geographic areas have contributed to its ongoing evolution and dissemination.

Keyword :

Bayesian phylodynamics Bayesian phylodynamics tomato chlorosis virus tomato chlorosis virus virus dispersal virus dispersal

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GB/T 7714 Wu, Kangcheng , Zhang, Shiwei , Huang, Wende et al. Phylogeographic History of Tomato Chlorosis Virus [J]. | VIRUSES-BASEL , 2025 , 17 (4) .
MLA Wu, Kangcheng et al. "Phylogeographic History of Tomato Chlorosis Virus" . | VIRUSES-BASEL 17 . 4 (2025) .
APA Wu, Kangcheng , Zhang, Shiwei , Huang, Wende , Du, Zhenguo , Gao, Fangluan , Guan, Xiayu . Phylogeographic History of Tomato Chlorosis Virus . | VIRUSES-BASEL , 2025 , 17 (4) .
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Three New Species of Russulaceae (Russulales, Basidiomycota) from Southern China SCIE
期刊论文 | 2024 , 10 (1) | JOURNAL OF FUNGI
WoS CC Cited Count: 7
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The characterization of natural fungal diversity impacts our understanding of ecological and evolutionary processes and can lead to novel bioproduct discovery. Russula and Lactarius, both in the order Russulales, represent two large genera of ectomycorrhizal fungi that include edible as well as toxic varieties. Based on morphological and phylogenetic analyses, including nucleotide sequences of the internal transcribed spacer (ITS), the 28S large subunit of ribosomal RNA (LSU), the second largest subunit of RNA polymerase II (RPB2), the ribosomal mitochondrial small subunit (mtSSU), and the translation elongation factor 1-alpha (TEF1-alpha) gene sequences, we here describe and illustrate two new species of Russula and one new species of Lactarius from southern China. These three new species are: R. junzifengensis (R. subsect. Virescentinae), R. zonatus (R. subsect. Crassotunicatae), and L. jianyangensis (L. subsect. Zonarii).

Keyword :

Basidiomycota Basidiomycota Lactarius Lactarius morphological and phylogenetic analyses morphological and phylogenetic analyses new species new species Russula Russula Russulales Russulales

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GB/T 7714 Liu, Sen , Zhu, Mengjia , Keyhani, Nemat O. et al. Three New Species of Russulaceae (Russulales, Basidiomycota) from Southern China [J]. | JOURNAL OF FUNGI , 2024 , 10 (1) .
MLA Liu, Sen et al. "Three New Species of Russulaceae (Russulales, Basidiomycota) from Southern China" . | JOURNAL OF FUNGI 10 . 1 (2024) .
APA Liu, Sen , Zhu, Mengjia , Keyhani, Nemat O. , Wu, Ziyi , Lv, Huajun , Heng, Zhiang et al. Three New Species of Russulaceae (Russulales, Basidiomycota) from Southern China . | JOURNAL OF FUNGI , 2024 , 10 (1) .
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Phylogenetic and Morphological Evidence for Three New Species of Diaporthales (Ascomycota) from Fujian Province, China SCIE
期刊论文 | 2024 , 10 (6) | JOURNAL OF FUNGI
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Members of the fungal order Diaporthales are sac fungi that include plant pathogens (the notorious chestnut blight fungus), as well as saprobes and endophytes, and are capable of colonizing a wide variety of substrates in different ecosystems, habitats, and hosts worldwide. However, many Diaporthales species remain unidentified, and various inconsistencies within its taxonomic category remain to be resolved. Here, we aimed to identify and classify new species of Diaporthales by using combined morphological and molecular characterization and coupling this information to expand our current phylogenetic understanding of this order. Fungal samples were obtained from dead branches and diseasedleaves of Camellia (Theaceae) and Castanopsis (Fagaceae) in Fujian Province, China. Based on morphological characteristics and molecular phylogenetic analyses derived from the combined nucleotide sequences of loci of the internal transcribed spacer regions with the intervening 5.8S nrRNA gene (ITS), the 28S large subunit of nuclear ribosomal RNA gene (LSU), the translation elongation factor 1-alpha gene (tef1), the partial beta-tubulin gene (tub2), and partial RNA polymerase II second-largest subunit gene (rpb2), three new species of Diaporthales were identified and characterized. They are as follows: Chrysofolia camelliae sp. nov., Dendrostoma castanopsidis sp. nov., and Pseudoplagiostoma wuyishanense sp. nov. They are described and illustrated. This study extends our understanding of species diversity within the Diaporthales.

Keyword :

multigene phylogeny multigene phylogeny phytopathogenic fungi phytopathogenic fungi Pseudoplagiostomataceae Pseudoplagiostomataceae taxonomy taxonomy

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GB/T 7714 Mu, Taichang , Lin, Yongsheng , Keyhani, Nemat O. et al. Phylogenetic and Morphological Evidence for Three New Species of Diaporthales (Ascomycota) from Fujian Province, China [J]. | JOURNAL OF FUNGI , 2024 , 10 (6) .
MLA Mu, Taichang et al. "Phylogenetic and Morphological Evidence for Three New Species of Diaporthales (Ascomycota) from Fujian Province, China" . | JOURNAL OF FUNGI 10 . 6 (2024) .
APA Mu, Taichang , Lin, Yongsheng , Keyhani, Nemat O. , Pu, Huili , Lv, Ziying , Lan, Chenhui et al. Phylogenetic and Morphological Evidence for Three New Species of Diaporthales (Ascomycota) from Fujian Province, China . | JOURNAL OF FUNGI , 2024 , 10 (6) .
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Molecular phylogenetic and estimation of evolutionary divergence and biogeography of the family Schizoparmaceae and allied families (Diaporthales, Ascomycota) SCIE
期刊论文 | 2024 , 201 | MOLECULAR PHYLOGENETICS AND EVOLUTION
WoS CC Cited Count: 5
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The Diaporthales includes 32 families, many of which are important plant pathogens, endophytes and saprobes, e.g., members of the families Pseudoplagiostomataceae, Pyrisporaceae and Schizoparmaceae. Nucleotide sequences derived from five genetic loci including: ITS, LSU, TEF1-alpha, TUB2 and RPB2 were used for Bayesian evolutionary analysis to determine divergence times and evolutionary relationships within the Schizoparmaceae. Molecular clock analyses revealed that the ancestor of Schizoparmaceae split during the Upper Cretaceous period approximately 75.7 Mya (95 % highest posterior density of 60.3-91.3 Mya). Reconstructing ancestral state in phylogenies (RASP) with using the Bayesian Binary Markov chain Monte Carlo (BBM) Method to reconstruct the historical biogeography for the family Schizoparmaceae indicated its most likely origin in Africa. Based on taxonomic and phylogenetic analyses, the Pseudoplagiostomataceae and Pyrisporaceae relationships were clarified and a total of four species described herein. For Pseudoplagiostomataceae, three new species and one known species that include, Pseudoplagiostoma fafuense sp. nov., Ps. ilicis sp. nov., Ps. sanmingense sp. nov. and Ps. bambusae are described and a key of Pseudoplagiostomataceae is provided. With respect to Pyrisporaceae, we considered Pseudoplagiostoma castaneae to be a synonym of Pyrispora castaneae. . In addition, a new species of Schizoparmaceae, Coniella fujianensis sp. nov. is described and illustrated.

Keyword :

African origin African origin Ancestral state reconstruction Ancestral state reconstruction Molecular clock Molecular clock Morphology Morphology Multilocus phylogeny Multilocus phylogeny Taxonomy Taxonomy

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GB/T 7714 Mu, Taichang , Lin, Yongsheng , Pu, Huili et al. Molecular phylogenetic and estimation of evolutionary divergence and biogeography of the family Schizoparmaceae and allied families (Diaporthales, Ascomycota) [J]. | MOLECULAR PHYLOGENETICS AND EVOLUTION , 2024 , 201 .
MLA Mu, Taichang et al. "Molecular phylogenetic and estimation of evolutionary divergence and biogeography of the family Schizoparmaceae and allied families (Diaporthales, Ascomycota)" . | MOLECULAR PHYLOGENETICS AND EVOLUTION 201 (2024) .
APA Mu, Taichang , Lin, Yongsheng , Pu, Huili , Keyhani, Nemat O. , Dang, Yuxiao , Lv, Huajun et al. Molecular phylogenetic and estimation of evolutionary divergence and biogeography of the family Schizoparmaceae and allied families (Diaporthales, Ascomycota) . | MOLECULAR PHYLOGENETICS AND EVOLUTION , 2024 , 201 .
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