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学者姓名：李煜
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Abstract ：
The Fagaceae family, comprising over 900 species, is an essential component of Northern Hemisphere forest ecosystems. However, genomic data for tropical and subtropical genera Castanopsis and Castanea remain limited compared to the well-studied oak. Here, we present chromosome-level genome assemblies of Castanopsis carlesii and Castanea henryi, with assembled genome sizes of 927.24 Mb (N50 = 1.57 Mb) and 780.10 Mb (N50 = 1.07 Mb), respectively, and repetitive sequence contents of 45.79% and 44.88%. Comparative genomic analysis revealed that the estimated divergence time between Castanopsis and Castanea was determined to be 48.3 Mya and provided evidence that both genera experienced only one of the ancient whole genome triplication event (gamma event) shared with most eudicots. The development of C. carlesii flower bracts and cupules was controlled by A- and E-class genes, suggesting that the cupules may originate from the bracts. Additionally, genes involved in sucrose and starch metabolism genes played distinct roles during C. carlesii fruit development. The amplification of the nucleotide-binding leucine-rich repeat (NLR) gene family in Fagaceae exhibited similarities, indicating that this expansion may be an adaptation to similar environmental pressures. This study provides valuable genomic resources for Asian Fagaceae and enhances our understanding of Fagaceae evolution.
Keyword ：
Fagaceae Fagaceae Genomics Genomics MADS-box genes MADS-box genes Resistance genes Resistance genes Sucrose-starch metabolism Sucrose-starch metabolism
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| GB/T 7714 | Tu, Xiong-De , Lin, Wen-Jun , Xin, Ya-Xuan et al. Genomic insights into Castanopsis carlesii and Castanea henryi: flower and fruit development and evolution of NLR genes in the beech-oak family [J]. | MOLECULAR HORTICULTURE , 2025 , 5 (1) . |
| MLA | Tu, Xiong-De et al. "Genomic insights into Castanopsis carlesii and Castanea henryi: flower and fruit development and evolution of NLR genes in the beech-oak family" . | MOLECULAR HORTICULTURE 5 . 1 (2025) . |
| APA | Tu, Xiong-De , Lin, Wen-Jun , Xin, Ya-Xuan , Fu, Hou-Hua , Zhou, Cheng-Yuan , Lin, Yi-Zhe et al. Genomic insights into Castanopsis carlesii and Castanea henryi: flower and fruit development and evolution of NLR genes in the beech-oak family . | MOLECULAR HORTICULTURE , 2025 , 5 (1) . |
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Glyptostrobus pensilis is an endangered tree species, and detecting its genetic diversity can reveal the mechanisms of endangerment, providing references for the conservation of genetic resources. Samples of 137 trees across seven populations within Fujian Province were collected and sequenced using double-digest restriction site-associated DNA (ddRAD-seq). A total of 3,687,189 single-nucleotide polymorphisms (SNPs) were identified, and 15,158 high-quality SNPs were obtained after filtering. The genetic diversity in the populations was found to be low (Ho = 0.08630, He = 0.03475, pi = 0.07239), with a high genetic differentiation coefficient (Fst). When K = 4, the coefficient of variation (CV) error value was minimized, suggesting that the 137 individuals could be divided into four groups, with frequent gene flow between them. Principal component analysis (PCA) divided the seven populations into two major categories based on their north-south geographic location. The clustering was consistent with those obtained from the PCA. The main reasons for the endangerment of G. pensilis are likely to be poor natural regeneration, human disturbances, and climatic factors. It is recommended that methods such as in situ conservation, ex situ conservation, and the establishment of germplasm banks be implemented to maintain the genetic diversity of G. pensilis populations.
Keyword ：
conservation conservation ddRAD-seq ddRAD-seq genetic diversity genetic diversity genetic structure genetic structure Glyptostrobus pensilis Glyptostrobus pensilis
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| GB/T 7714 | Huang, Yongrong , Li, Yu , Hong, Xiaojie et al. Genetic Variation for Wild Populations of the Rare and Endangered Plant Glyptostrobus pensilis Based on Double-Digest Restriction Site-Associated DNA Sequencing [J]. | CURRENT ISSUES IN MOLECULAR BIOLOGY , 2025 , 47 (1) . |
| MLA | Huang, Yongrong et al. "Genetic Variation for Wild Populations of the Rare and Endangered Plant Glyptostrobus pensilis Based on Double-Digest Restriction Site-Associated DNA Sequencing" . | CURRENT ISSUES IN MOLECULAR BIOLOGY 47 . 1 (2025) . |
| APA | Huang, Yongrong , Li, Yu , Hong, Xiaojie , Luo, Suzhen , Cai, Dedan , Xiao, Xiangxi et al. Genetic Variation for Wild Populations of the Rare and Endangered Plant Glyptostrobus pensilis Based on Double-Digest Restriction Site-Associated DNA Sequencing . | CURRENT ISSUES IN MOLECULAR BIOLOGY , 2025 , 47 (1) . |
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Tea oil is an important high-quality edible oil derived from woody plants. Camellia oleifera is the largest and most widely planted oil-producing plant in the Camellia genus in China, and its seeds are the most important source for obtaining tea oil. In current research, improving the yield and quality of tea oil is the main goal of oil tea genetic breeding. The aim of this study was to investigate the degree of genetic variation in an early crossing population of C. oleifera and identify single nucleotide polymorphisms (SNPs) and genes significantly associated with fruit traits, which can provide a basis for marker-assisted selection and gene editing for achieving trait improvement in the future. In this study, we selected a crossing population of approximately 40-year-old C. oleifera with a total of 330 samples. Then, ddRAD sequencing was used for SNP calling and population genetic analysis, and association analysis was performed on fruit traits measured repeatedly for two consecutive years. The research results indicate that over 8 million high-quality SNPs have been identified, but the vast majority of SNPs occur in intergenic regions. The nucleotide polymorphism of this population is at a low level, and Tajima's D values are mostly greater than 0, indicating that the change in this population was not suitable for the model of central evolution. The population structure analysis shows that the population has seven theoretical sources of genetic material and can be divided into seven groups, and the clustering analysis results support the population structure analysis results. Association analysis identified significant SNPs associated with genes related to the seed number of a single fruit and seed kernel oil content. Our findings provide a basis for molecular breeding and future genetic improvement of cultivated oil tea.
Keyword ：
association analysis association analysis Camellia oleifera Camellia oleifera crossing population crossing population genetic variation genetic variation SNP SNP
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| GB/T 7714 | Zhou, Lexin , Li, Yu , Ye, Ling et al. Genetic Variation in a Crossing Population of Camellia oleifera Based on ddRAD Sequencing and Analysis of Association with Fruit Traits [J]. | CURRENT ISSUES IN MOLECULAR BIOLOGY , 2025 , 47 (2) . |
| MLA | Zhou, Lexin et al. "Genetic Variation in a Crossing Population of Camellia oleifera Based on ddRAD Sequencing and Analysis of Association with Fruit Traits" . | CURRENT ISSUES IN MOLECULAR BIOLOGY 47 . 2 (2025) . |
| APA | Zhou, Lexin , Li, Yu , Ye, Ling , Li, Jiani , Liang, Tian , Liu, Yanxuan et al. Genetic Variation in a Crossing Population of Camellia oleifera Based on ddRAD Sequencing and Analysis of Association with Fruit Traits . | CURRENT ISSUES IN MOLECULAR BIOLOGY , 2025 , 47 (2) . |
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油茶是中国重要的木本油料树种，具有较高的利用价值。本研究利用SRAP分子标记对10个福建省主栽普通油茶优良无性系进行分析，探讨其遗传差异，结果表明：从256对SRAP引物组合中筛选出12对引物对10个优良无性系进行PCR扩增，共获得186个条带，其中多态性条带166个，多态性条带的比率为89.2%。EM-2/ME-4引物组合扩增的条带多态性最高，为100%；多态性信息量最小值和最大值分别为0.280和0.394，多态性信息量平均值为0.330。10个优良无性系遗传相似系数最小值和最大值分别为0.591 4和0.774 2，遗传相似系数平均值为0.670 8。聚类分析表明，遗传相似系数为0.677 5处，10个普通油茶优良无性系可以划为4大类：闽43和闽46聚为第Ⅰ类，闽48、闽49、闽53聚为第Ⅱ类，闽50聚为第Ⅲ类，闽54、闽56、闽60、闽62聚为第Ⅳ类。这10个优良无性系之间存在着较高的遗传多样性水平，研究结果为普通油茶杂交育种和分子育种工作的开展提供参考。
Keyword ：
SRAP SRAP 无性系 无性系 普通油茶 普通油茶 遗传差异 遗传差异
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| GB/T 7714 | 王庭雄 , 李祥胜 , 梁诗强 et al. 福建省主栽普通油茶优良无性系遗传差异的SRAP分析 [J]. | 分子植物育种 , 2024 , 22 (17) : 5663-5669 . |
| MLA | 王庭雄 et al. "福建省主栽普通油茶优良无性系遗传差异的SRAP分析" . | 分子植物育种 22 . 17 (2024) : 5663-5669 . |
| APA | 王庭雄 , 李祥胜 , 梁诗强 , 叶树涛 , 吕欢欢 , 陈辉 et al. 福建省主栽普通油茶优良无性系遗传差异的SRAP分析 . | 分子植物育种 , 2024 , 22 (17) , 5663-5669 . |
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‘油榛’是福建省建瓯市广泛种植的优良农家品种之一。本研究利用SSR分子标记对‘油榛’栽培群体进行遗传多样性分析，探索其变异情况，结果表明：由10个SSR引物组合对锥栗农家品种‘油榛’栽培群体的30个植株进行PCR扩增，共计扩增出26个条带，多态性条带26个，多态性条带占比100%,30个植株的遗传相似性系数在0.115 4～1.000 0之间，平均为0.877 2，表明锥栗农家品种‘油榛’具有较高的遗传多样性。为了解‘油榛’栽培群体30个植株的亲缘关系，对30个植株进行聚类分析，其中YZ5、YZ1单独各聚为一类，其余28个植株聚为一类。研究结果为在锥栗农家品种栽培群体中进行优树选择提供了理论依据。
Keyword ：
SSR SSR 农家品种 农家品种 油榛 油榛 遗传多样性 遗传多样性 锥栗 锥栗
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| GB/T 7714 | 叶树涛 , 方周 , 李颖林 et al. 锥栗农家品种‘油榛’栽培群体的SSR变异分析 [J]. | 分子植物育种 , 2024 , 22 (05) : 1508-1513 . |
| MLA | 叶树涛 et al. "锥栗农家品种‘油榛’栽培群体的SSR变异分析" . | 分子植物育种 22 . 05 (2024) : 1508-1513 . |
| APA | 叶树涛 , 方周 , 李颖林 , 吴钧剑 , 郑国华 , 陈辉 et al. 锥栗农家品种‘油榛’栽培群体的SSR变异分析 . | 分子植物育种 , 2024 , 22 (05) , 1508-1513 . |
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Tissue culture processes have been established for Dendrobium wilsonii Rolfe, a valued ornamental and medicinal orchid. Pods aged 180 days served as seed sources. These seeds underwent surface sterilization and were then aseptically germinated on Murashige and Skoog (MS) medium supplemented with 0.5 mg L-1 Benzyl adenine (BA), 0.5 mg L-1 Naphthalene acetic acid (NAA), 20 g L-1 Sucrose (Su), 7 g L-1 Agar, 1 g L-1 Activated charcoal (AC), 1 g L-1 Peptone and 1 g L-1 Hyponex No.1 incubated in a growth chamber at 25.2 degrees C with a 12-hour photoperiod and a light intensity of 2200 lx. Multiple shoot induction and proliferation was on MS medium in vitro produced shoots were rooted on MS medium with Hyponex No.1, 25 g L-1 Su, 1 g L-1 AC and 1.5 g L-1 Peptone. Peptone enhanced seedling growth and promoted clumped shoot development. The establishment of Dendrobium wilsonii Rolfe regeneration system provides a methodology for conserving germplasm resources and for commercial propagules production.
Keyword ：
Dendrobium wilsonii Rolfe Dendrobium wilsonii Rolfe Regeneration system Regeneration system Tissue culture Tissue culture
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| GB/T 7714 | Lin, Wei , Li, Yu , Liang, Jincheng et al. Establishment of Dendrobium wilsonii Rolfe in vitro regeneration system [J]. | SCIENTIA HORTICULTURAE , 2024 , 324 . |
| MLA | Lin, Wei et al. "Establishment of Dendrobium wilsonii Rolfe in vitro regeneration system" . | SCIENTIA HORTICULTURAE 324 (2024) . |
| APA | Lin, Wei , Li, Yu , Liang, Jincheng , Liu, Yufang , Chen, Peiqiang , He, Bizhu et al. Establishment of Dendrobium wilsonii Rolfe in vitro regeneration system . | SCIENTIA HORTICULTURAE , 2024 , 324 . |
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为筛选出生长良好且栗疫病抗性强的优良家系和优良单株，对12个锥栗半同胞家系的栗疫病抗性相关性状及生长性状进行变异分析、方差分析、相关性分析和遗传参数估算。结果表明，12个锥栗半同胞家系各性状的平均变异系数为20.19%，病斑面积的变异系数最大，为39.76%，叶形指数的变异系数最小，为11.14%。病斑宽、叶面积、叶周长、叶长、叶脉数在家系间具有显著差异(P<0.05)，感病率、感病指数、叶宽在家系间具有极显著差异(P<0.01)。苗高与感病率呈显著负相关(P<0.05)，与感病指数呈极显著负相关(P<0.01);叶长与感病率、感病指数呈极显著负相关(P<0.01)。筛选出JO14(‘白露仔’)、JO8(‘油桐仔’)、JO2(‘大苞榛’) 3个优良家系;筛选出9株(JO2-25、JO13-29、JO14-32、JO2-12、JO8-17、JO3-4、JO5-26、JO2-19、JO12-20)栗疫病抗性强的优良单株，4株(JO2-5、JO7-16、JO8-17、JO14-3)生长良好的优良单株，其中JO8-17抗性强且生长良好。
Keyword ：
优良单株 优良单株 优良家系 优良家系 半同胞家系 半同胞家系 栗疫病抗性 栗疫病抗性 锥栗 锥栗
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| GB/T 7714 | 吴美珍 , 李佳妮 , 李煜 et al. 锥栗半同胞家系栗疫病抗性与生长性状的遗传分析 [J]. | 森林与环境学报 , 2024 , 44 (02) : 175-182 . |
| MLA | 吴美珍 et al. "锥栗半同胞家系栗疫病抗性与生长性状的遗传分析" . | 森林与环境学报 44 . 02 (2024) : 175-182 . |
| APA | 吴美珍 , 李佳妮 , 李煜 , 吴钧剑 , 杨金辉 , 陈辉 . 锥栗半同胞家系栗疫病抗性与生长性状的遗传分析 . | 森林与环境学报 , 2024 , 44 (02) , 175-182 . |
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【目的】筛选锥栗优良授粉组合与杂交组合。【方法】以5个锥栗主栽优良农家品种为试验材料进行杂交授粉，对所得果实性状进行变异分析。【结果】16个杂交组合果实性状间变异幅度最大的为栗苞质量、坚果质量，变异幅度最小为果型指数，不同组合间性状差异显著，具有较好的选择潜力；总苞质量、坚果质量、坚果长、坚果宽、出籽率等性状间具有不同程度的正相关性，可进行联合选择；总苞质量、坚果质量、栗苞长、栗苞宽、栗苞高、出籽率等性状存在不同程度的花粉直感效应；以亲本ZL1为母本、亲本ZL12为父本的各授粉组合果实性状表现优良，并选择出5个优良授粉组合；ZL1的GCA效应较高，而杂交组合ZL1×ZL9、ZL6×ZL12、ZL3×ZL6的SCA效应最高。【结论】研究结果可为锥栗杂交育种和丰产栽培提供参考。
Keyword ：
人工授粉 人工授粉 农家品种 农家品种 变异分析 变异分析 杂交育种 杂交育种 锥栗 锥栗
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| GB/T 7714 | 方周 , 顾光仕 , 李煜 et al. 锥栗农家品种人工授粉杂交果实性状的变异分析 [J]. | 福建农林大学学报(自然科学版) , 2024 , 53 (01) : 1-6 . |
| MLA | 方周 et al. "锥栗农家品种人工授粉杂交果实性状的变异分析" . | 福建农林大学学报(自然科学版) 53 . 01 (2024) : 1-6 . |
| APA | 方周 , 顾光仕 , 李煜 , 杨金辉 , 江锡兵 , 陈辉 . 锥栗农家品种人工授粉杂交果实性状的变异分析 . | 福建农林大学学报(自然科学版) , 2024 , 53 (01) , 1-6 . |
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Qinting Lake Park has effectively imported Rhododendron varieties from Zhejiang Province. The analytic hierarchy process was employed to devise an evaluation framework to evaluate the ornamental and adaptive features of these species. Subsequently, we conducted a standardized evaluation of 24 species for their ornamental and adaptive traits under controlled cultivation conditions. The findings indicated that the percentage of ornamental flowers in the first-level index was significantly greater than the other two factors, indicating that the ornamental value of flowers was the most important in the evaluation of Rhododendron ornamental value. Among the secondary indicators, the proportion of flower color and flower weight was significantly higher than that of other factors, which had the greatest impact on the evaluation results. The 24 Rhododendron species were classified into two grades based on their ornamental value, as determined by index weights and scoring standards. Rhododendron 'Xueqing', Rhododendron 'Big Qinglian', and Rhododendron 'Jinyang No. 9' exhibited superior ornamental value and demonstrated more favorable suitability for garden applications.
Keyword ：
adaptability adaptability analytic hierarchy process analytic hierarchy process comprehensive evaluation comprehensive evaluation ornamentality ornamentality Rhododendron Rhododendron
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| GB/T 7714 | Liang, Jincheng , Chen, Yaoli , Tang, Xuexiao et al. Comprehensive Evaluation of Appreciation of Rhododendron Based on Analytic Hierarchy Process [J]. | PLANTS-BASEL , 2024 , 13 (4) . |
| MLA | Liang, Jincheng et al. "Comprehensive Evaluation of Appreciation of Rhododendron Based on Analytic Hierarchy Process" . | PLANTS-BASEL 13 . 4 (2024) . |
| APA | Liang, Jincheng , Chen, Yaoli , Tang, Xuexiao , Lu, Yin , Yu, Jinghui , Wang, Zongbo et al. Comprehensive Evaluation of Appreciation of Rhododendron Based on Analytic Hierarchy Process . | PLANTS-BASEL , 2024 , 13 (4) . |
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【目的】利用基因组和转录组数据鉴定分析花发育相关的MADS-box基因家族成员的结构特征及表达模式，为柳属花被缺失的分子机制研究提供理论依据。【方法】通过生物信息学方法鉴定柳属的长梗柳(Salix dunnii)、欧蒿柳(S.viminalis)、红皮柳(S.purpurea)的MADS-box基因家族成员，并对3种柳树花发育相关的MADS-box基因系统发育关系、基因复制和丢失、基因结构和理化性质进行分析。根据雌雄花芽的转录组数据分析长梗柳花发育相关基因在花芽不同发育阶段的表达模式。【结果】在长梗柳、欧蒿柳和红皮柳中分别鉴定出82、82和98个MADS-box家族基因。3种柳树的花发育相关基因经历了基因复制和丢失，相同亚类基因结构和保守结构域相似，但部分A、B、C和E亚类基因的K-box结构域缺失。长梗柳花发育相关基因的表达结果显示，在雌花和雄花中具有不同的表达模式。其中，B亚类SdMADS26和SdMADS4基因表达量均偏低。【结论】柳属植物花发育相关基因中部分成员的基因结构和表达模式发生了变化，推测这些变化可能与柳属花被缺失相关。
Keyword ：
MADS-box基因家族 MADS-box基因家族 基因组 基因组 柳属 柳属 花被 花被 转录组 转录组
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| GB/T 7714 | 王媛 , 翁慧莹 , 曾思文 et al. 柳属植物花发育相关的MADS-box基因家族成员鉴定与表达分析 [J]. | 福建农林大学学报(自然科学版) , 2024 , 53 (03) : 355-363 . |
| MLA | 王媛 et al. "柳属植物花发育相关的MADS-box基因家族成员鉴定与表达分析" . | 福建农林大学学报(自然科学版) 53 . 03 (2024) : 355-363 . |
| APA | 王媛 , 翁慧莹 , 曾思文 , 王苑 , 李煜 , 何理 . 柳属植物花发育相关的MADS-box基因家族成员鉴定与表达分析 . | 福建农林大学学报(自然科学版) , 2024 , 53 (03) , 355-363 . |
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