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学者姓名：曹世江
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Abstract ：
本研究通过克隆、生物信息学和表达分析方法对杉木热激蛋白70（heat shock protein 70,HSP70）基因进行研究，为深入理解杉木HSP70基因的功能和杉木遗传改良以及其可持续栽培提供科学依据。本研究选用杉木优良无性系“洋061”一年生苗作为试验材料，利用逆转录聚合酶链式反应（RT-PCR）克隆得到ClHSP70基因。运用Expasy软件等在线软件预测并分析ClHSP70蛋白的理化性质、跨膜螺旋域、信号肽、二级结构和三级结构；运用Cell-PLoc 2.0在线软件预测蛋白的亚细胞定位；运用Mega 11软件构建系统发育树；克隆ClHSP70基因并构建到pCAMBIA35s-EGFP载体中，分析ClHSP70蛋白的亚细胞定位；并利用实时荧光定量PCR（quantitative real-time PCR）对其表达水平进行分析。克隆得到的ClHSP70基因编码670个氨基酸，ClHSP70蛋白的分子式为C
Keyword ：
ClHSP70基因 ClHSP70基因 亚细胞定位 亚细胞定位 基因克隆 基因克隆 杉木 杉木 高温与干旱胁迫 高温与干旱胁迫
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| GB/T 7714 | 郭胜周 , 许祖元 , 刘荣林 et al. 杉木ClHSP70基因的克隆及表达模式分析 [J]. | 广西师范大学学报(自然科学版) , 2025 , 43 (04) : 213-223 . |
| MLA | 郭胜周 et al. "杉木ClHSP70基因的克隆及表达模式分析" . | 广西师范大学学报(自然科学版) 43 . 04 (2025) : 213-223 . |
| APA | 郭胜周 , 许祖元 , 刘荣林 , 林秦民 , 曹光球 , 曹世江 . 杉木ClHSP70基因的克隆及表达模式分析 . | 广西师范大学学报(自然科学版) , 2025 , 43 (04) , 213-223 . |
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为了解不同林分密度对杉木Cunninghamia lanceolate人工林土壤特性的影响，以福建省洋口国有林场的不同林分密度的实验林为研究对象，对不同林分密度下杉木人工林的土壤含水量、化学计量比、酶活及林下植被多样性进行研究，结果表明：1)各个密度对杉木人工林林下植被多样性中的物种丰富度指数和Shannon-Wiener指数无显著性影响(P>0.05),对Simpson指数和Pielou均匀度指数却有较大影响。2)不同林分密度对土壤化学性质有显著差异(P<0.05),随着林分密度的上升，土壤全碳含量先增加后减少，土壤全氮先下降后上升。3)不同林分密度对于土壤酶活性具有显著影响(P<0.05),其中，随着林分密度的上升，土壤的多酚氧化酶、酸性磷酸酶活性降低，而土壤脲酶和土壤蔗糖酶活性增加。4)不同林分密度对土壤微生物优势菌群存在显著影响(P<0.05),低林分密度一定程度上可以提高土壤微生物细菌群落结构多样性。在土壤细菌群落与理化性质相关性分析中，全磷(TP)、有效磷(AP)含量与土壤优势菌群Actinobacteria呈极显著相关(P<0.01)。全碳(TC)、全氮(TN)与优势菌群Zoopagomycota呈极显著相关(P<0.01)。总体而言，低林分密度是杉木人工林种植的最佳选择。
Keyword ：
土壤微生物 土壤微生物 土壤酶活 土壤酶活 杉木人工林 杉木人工林 林分密度 林分密度 植被多样性 植被多样性
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| GB/T 7714 | 左晓东 , 汪星星 , 许祖元 et al. 不同林分密度对杉木林土壤特性及林下植被的影响 [J]. | 广西师范大学学报(自然科学版) , 2025 , 43 (04) : 201-212 . |
| MLA | 左晓东 et al. "不同林分密度对杉木林土壤特性及林下植被的影响" . | 广西师范大学学报(自然科学版) 43 . 04 (2025) : 201-212 . |
| APA | 左晓东 , 汪星星 , 许祖元 , 郑宏 , 曹光球 , 曹世江 . 不同林分密度对杉木林土壤特性及林下植被的影响 . | 广西师范大学学报(自然科学版) , 2025 , 43 (04) , 201-212 . |
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Phoebe bournei is an endangered and protected precious tree in China, valued both for high-quality timber and an ornamental property. Abiotic stress greatly affects the survival of Phoebe species. The basic leucine zipper (bZIP) transcription factor is evolutionarily conserved and plays a critical role in abiotic stress signaling in plants. However, studies on Phoebe bournei bZIP (PbbZIP) genes remain elusive. To determine the adaptation roles of the bZIP family, we performed a genome identification and expression profile analysis in P. bournei. A total of 71 PbbZIP genes were identified across 12 chromosomes and divided into 12 major subgroups by phylogenetic analysis, which revealed strong collinearity with Arabidopsis thaliana chromosomes. In addition, an analysis of their physicochemical characteristics indicated nuclear localization for all PbbZIP proteins, with most exhibiting hydrophilicity or amphiphilic features. The PbbZIP genes play a crucial role in stress responses. Specifically, at least five of these genes exhibit dynamic expression patterns in response to temperature, drought, and salt stress. Notably, PbbZIP 67 shows significantly elevated expression levels under both saline and heat conditions. Moreover, PbbZIP 32 and PbbZIP 14 display notably higher expression levels under heat and drought conditions, respectively, compared to normal conditions. These findings indicate that they play important roles in stress tolerance, highlighting their significance in helping organisms adapt to adverse environmental factors. This study sheds light on the evolution and functional adaptation of PbbZIP genes and provides valuable resources for promoting the stress tolerance of Phoebe bournei. © 2025, The Authors. All rights reserved.
Keyword ：
Abiotic Abiotic Biotic Biotic Plant diseases Plant diseases Transcription Transcription
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| GB/T 7714 | Zhang, Zhenzhen , Bakari, Almas , Lv, Mengmeng et al. Genome-Wide Identification of Bzip Genes in Phoebe Bournei and Their Roles in Response to Abiotic Stress [J]. | SSRN , 2025 . |
| MLA | Zhang, Zhenzhen et al. "Genome-Wide Identification of Bzip Genes in Phoebe Bournei and Their Roles in Response to Abiotic Stress" . | SSRN (2025) . |
| APA | Zhang, Zhenzhen , Bakari, Almas , Lv, Mengmeng , Guan, Hengfeng , Xu, Menglan , Zhang, Linping et al. Genome-Wide Identification of Bzip Genes in Phoebe Bournei and Their Roles in Response to Abiotic Stress . | SSRN , 2025 . |
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The CPP gene family comprises transcription factor genes containing a conserved CRC domain, which is mainly involved in plant development and evolution. Although CPP genes have been widely studied in many plants, little is known about them in woody plants, especially in the endangered species Phoebe bournei (Hemsl.). In the genome of Phoebe bournei, we identified 11 PbCPP genes (PbCPP1-PbCPP11) distributed on four chromosomes, with large differences in the number of amino acids. They encode both acidic and alkaline proteins. A phylogenetic analysis showed that these PbCPP genes can be divided into three subfamilies, A, B, and C, which contain seven, two, and two genes, respectively. Through an interspecific collinearity analysis, we identified homologous PbCPP genes. A promoter cis-acting element analysis revealed that PbCPPs contain a variety of elements that respond to plant hormones, stress signals, and light and play a role in growth and development, and most PbCPP genes (except PbCPP3 and PbCPP8) contain MYB binding site elements that regulate drought-induced stress responses, indicating that they play an important role in plant drought resistance. An expression analysis showed that PbCPP3 and PbCPP4 expression was high in the roots and stems and lower in the leaves, whereas the expression of most of the other genes was low in the roots, stems, and leaves. In addition, six representative PbCPP genes were detected using qRT-PCR. The results show significant differences in the expression of PbCPP genes under abiotic stress conditions (drought, cold, and salt), indicating that they play an important role in stress responses. This study preliminarily verified the role of the PbCPP gene family in different abiotic stress responses, which is of great significance for understanding its mechanism in plant growth and development and stress adaptation.
Keyword ：
abiotic stress abiotic stress CPP gene family CPP gene family expression analysis expression analysis genome-wide identification genome-wide identification Phoebe bournei Phoebe bournei
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| GB/T 7714 | Liu, Ronglin , Feng, Yizhuo , Li, Qingyan et al. Genome-Wide Analysis of CPP Transcription Factor Family in Endangered Plant Phoebe bournei and Its Response to Adversity [J]. | PLANTS-BASEL , 2025 , 14 (5) . |
| MLA | Liu, Ronglin et al. "Genome-Wide Analysis of CPP Transcription Factor Family in Endangered Plant Phoebe bournei and Its Response to Adversity" . | PLANTS-BASEL 14 . 5 (2025) . |
| APA | Liu, Ronglin , Feng, Yizhuo , Li, Qingyan , Wu, Hua , Guo, Shengzhou , Li, Junnan et al. Genome-Wide Analysis of CPP Transcription Factor Family in Endangered Plant Phoebe bournei and Its Response to Adversity . | PLANTS-BASEL , 2025 , 14 (5) . |
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The tea plant (Camellia sinensis) is an economically important crop that plays an important role not only in the beverage industry but also in the pharmaceutical industry. The environment has a great influence on the quality of the tea plant. Heat shock factors (Hsfs) are transcriptional regulators that control the plant response to adversity. However, only a limited number of studies have reported the Hsf gene in Camellia sinensis, and most of these reports involve high-temperature, drought, and salt stress. Research on light, dark, and cold stress is limited. In this study, 22 CsHsf genes were obtained by whole genome sequencing and found to be located on 11 chromosomes. In addition, the gene structure, protein motif, and phylogeny were studied. We classified the genes into three major subfamilies: CsHsfA, CsHsfB, and CsHsfC. Interestingly, we found that there was more alignment between CsHsf and Hsf genes in dicotyledons, including Arabidopsis thaliana and Solanum lycopersicum, than in the monocotyledon Oryza sativa. The expression of many CsHsf genes was affected by low-temperature, light, and dark abiotic stresses. Notably, CsHsf15 and CsHsf16 showed high induction rates under both light and cold stress, and both genes carried cis-acting elements associated with light and low-temperature responses. These results lay a solid groundwork for further investigations into the involvement of CsHsf genes in the response of Camellia sinensis to abiotic stresses.
Keyword ：
abiotic stress abiotic stress gene family gene family Hsf Hsf tea tea
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| GB/T 7714 | Li, Guimin , Shi, Xinying , Lin, Qinmin et al. Genome-Wide Identification and Expression Analysis of Heat Shock Transcription Factors in Camellia sinensis Under Abiotic Stress [J]. | PLANTS-BASEL , 2025 , 14 (5) . |
| MLA | Li, Guimin et al. "Genome-Wide Identification and Expression Analysis of Heat Shock Transcription Factors in Camellia sinensis Under Abiotic Stress" . | PLANTS-BASEL 14 . 5 (2025) . |
| APA | Li, Guimin , Shi, Xinying , Lin, Qinmin , Lv, Mengmeng , Chen, Jing , Wen, Yingxin et al. Genome-Wide Identification and Expression Analysis of Heat Shock Transcription Factors in Camellia sinensis Under Abiotic Stress . | PLANTS-BASEL , 2025 , 14 (5) . |
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Tieguanyin tea, a renowned oolong tea, is one of the ten most famous teas in China. The Squamosa Promoter Binding Protein (SBP)-box transcription factor family, widely present in plants, plays a crucial role in plant development, growth, and stress responses. In this study, we identify and analyze 22 CsSBP genes at the genome-wide level. These genes were distributed unevenly across 11 chromosomes. Using Arabidopsis thaliana and Solanum lycopersicum L. as model organisms, we constructed a phylogenetic tree to classify these genes into six distinct subfamilies. Collinearity analysis revealed 20 homologous gene pairs between AtSBP and CsSBP, 21 pairs between SiSBP and CsSBP, and 14 pairs between OsSBP and CsSBP. Cis-acting element analysis indicated that light-responsive elements were the most abundant among the CsSBP genes. Protein motif, domain, and gene architecture analyses demonstrated that members of the same subgroup shared similar exon-intron structures and motif arrangements. Furthermore, we evaluated the expression profiles of nine CsSBP genes under light, shade, and cold stress using qRT-PCR analysis. Notably, CsSBP1, CsSBP17, and CsSBP19 were significantly upregulated under all three stresses. This study provides fundamental insights into the CsSBP gene family and offers a novel perspective on the mechanisms of SBP transcription factor-mediated stress responses, as well as Tieguanyin tea's adaptation to environmental variations.
Keyword ：
abiotic stress abiotic stress gene family gene family SBP SBP tea tea Tieguanyin Tieguanyin
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| GB/T 7714 | Gao, Yusen , Wen, Yingxin , Lin, Qinmin et al. Phylogenetic and Expression Analysis of SBP-Box Gene Family to Enhance Environmental Resilience and Productivity in Camellia sinensis cv. Tie-guanyin [J]. | PLANTS-BASEL , 2025 , 14 (3) . |
| MLA | Gao, Yusen et al. "Phylogenetic and Expression Analysis of SBP-Box Gene Family to Enhance Environmental Resilience and Productivity in Camellia sinensis cv. Tie-guanyin" . | PLANTS-BASEL 14 . 3 (2025) . |
| APA | Gao, Yusen , Wen, Yingxin , Lin, Qinmin , Feng, Yizhuo , Shi, Xinying , Xiao, Siyao et al. Phylogenetic and Expression Analysis of SBP-Box Gene Family to Enhance Environmental Resilience and Productivity in Camellia sinensis cv. Tie-guanyin . | PLANTS-BASEL , 2025 , 14 (3) . |
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Heat stress transcription factors (HSFs) play a critical role in orchestrating cellular responses to elevated temperatures and various stress conditions. While extensively studied in model plants, the HSF gene family in Betula platyphylla remains unexplored, despite the availability of its sequenced genome. In this study, we employed bioinformatics approaches to identify 21 BpHSF genes within the Betula platyphylla genome, revealing their uneven distribution across chromosomes. These genes were categorized into three subfamilies: A, B, and C. Each was characterized by conserved protein motifs and gene structures, with notable divergence observed between subfamilies. Collinearity analysis suggested that segmental duplication events have driven the evolutionary expansion of the BpHSF gene family. Promoter region analysis identified an array of cis-acting elements linked to growth, development, hormonal regulation, and stress responses. Subcellular localization experiments confirmed the nuclear localization of BpHSFA2a, BpHSFB1a, and BpHSFC1a, consistent with in silico predictions. RNA-seq and RT-qPCR analyses revealed tissue-specific expression patterns of BpHSF genes and their dynamic responses to heat stress, with qPCR validation highlighting a significant upregulation of BpHSFA2a under high-temperature conditions. In summary, this study provided a comprehensive characterization of the HSF gene family in Betula platyphylla, laying a solid foundation for future functional studies. Particularly, BpHSFA2a emerges as a promising candidate gene for enhancing heat tolerance in Betula platyphylla, warranting further detailed investigation.
Keyword ：
Betula platyphylla Betula platyphylla gene expression gene expression heat stress transcription factor heat stress transcription factor high-temperature stress high-temperature stress subcellular localization subcellular localization
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| GB/T 7714 | Guo, Shengzhou , Chen, Hao , Wu, Hongwei et al. Genome-Wide Characterization of the Heat Shock Transcription Factor Gene Family in Betula platyphylla Reveals Promising Candidates for Heat Tolerance [J]. | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES , 2025 , 26 (1) . |
| MLA | Guo, Shengzhou et al. "Genome-Wide Characterization of the Heat Shock Transcription Factor Gene Family in Betula platyphylla Reveals Promising Candidates for Heat Tolerance" . | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 26 . 1 (2025) . |
| APA | Guo, Shengzhou , Chen, Hao , Wu, Hongwei , Xu, Zuyuan , Yang, Hao , Lin, Qinmin et al. Genome-Wide Characterization of the Heat Shock Transcription Factor Gene Family in Betula platyphylla Reveals Promising Candidates for Heat Tolerance . | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES , 2025 , 26 (1) . |
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As sustainable forest management gains increasing attention, comprehending the impact of stand density on soil properties and microbial communities is crucial for optimizing forest ecosystem functions. This study employed high-throughput sequencing in conjunction with soil physicochemical analysis to assess the effects of stand density on soil physicochemical properties and microbial community characteristics in Chinese fir plantations, aiming to elucidate the influence of density regulation on ecosystem services. Our results suggested that changes in soil physicochemical properties and microenvironmental conditions were key drivers of soil microbial diversity. Total carbon (TC), soluble nitrogen (SN), and light fraction organic matter decreased with increasing stand density, while total potassium (TK) and available phosphorus (AP) concentrations increased. The plot with a density of 900 trees ha-1 exhibited the highest bacterial diversity, in contrast to the plot with 1500 trees ha-1, which showed the lowest. The dominant microbial taxa were similar across different stand retention densities, with Acidobacteria, Proteobacteria, and Chloroflexi being the predominant bacterial phyla and Ascomycota and Basidiomycota being the main fungal groups. Significant positive correlations were observed between soil microbial community structures and environmental factors, particularly with respect to soil phosphorus and nitrogen content. The present study demonstrated that reduced stand densities modulated soil nutrient content and enhanced bacterial diversity, thereby contributing to a more complex and stable soil ecosystem structure. These insights provide a scientific foundation for optimizing the management of Chinese fir plantations, thereby supporting the sustainable development of forest ecosystems.
Keyword ：
Chinese fir Chinese fir high-throughput sequencing high-throughput sequencing microbial diversity microbial diversity plantation forest plantation forest soil microorganisms soil microorganisms
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| GB/T 7714 | Xu, Zuyuan , Fan, Fei , Lin, Qinmin et al. Effects of Different Stand Densities on the Composition and Diversity of Soil Microbiota in a Cunninghamia lanceolata Plantation [J]. | PLANTS-BASEL , 2025 , 14 (1) . |
| MLA | Xu, Zuyuan et al. "Effects of Different Stand Densities on the Composition and Diversity of Soil Microbiota in a Cunninghamia lanceolata Plantation" . | PLANTS-BASEL 14 . 1 (2025) . |
| APA | Xu, Zuyuan , Fan, Fei , Lin, Qinmin , Guo, Shengzhou , Li, Shumao , Zhang, Yunpeng et al. Effects of Different Stand Densities on the Composition and Diversity of Soil Microbiota in a Cunninghamia lanceolata Plantation . | PLANTS-BASEL , 2025 , 14 (1) . |
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【目的】探讨不同立地指数及密度下杉木幼龄林的养分流动和能量循环，筛选出养分与能量流动最快的立地指数和林分密度，为杉木幼龄林可持续经营提供重要理论基础。【方法】选取永安国有林场永浆工区7年生杉木人工林为试验林，采用双因素完全随机区组法设计，设置3种立地指数：16立地指数（S）、18立地指数（E）、20立地指数（T）；同时设置3种林分密度：1 200株·hm
Keyword ：
养分 养分 凋落物 凋落物 杉木 杉木 热值 热值
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| GB/T 7714 | 朱琴 , 关恒锋 , 杨浩 et al. 立地指数及密度对杉木幼龄林凋落物分解特征和热值的影响 [J]. | 中南林业科技大学学报 , 2025 , 45 (04) : 87-97 . |
| MLA | 朱琴 et al. "立地指数及密度对杉木幼龄林凋落物分解特征和热值的影响" . | 中南林业科技大学学报 45 . 04 (2025) : 87-97 . |
| APA | 朱琴 , 关恒锋 , 杨浩 , 许祖元 , 曹光球 , 曹世江 . 立地指数及密度对杉木幼龄林凋落物分解特征和热值的影响 . | 中南林业科技大学学报 , 2025 , 45 (04) , 87-97 . |
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[目的]杉木作为重要的用材树种,具有广泛的用途和经济价值。通过克隆、生物信息学分析和表达分析方法对杉木隐花色素CRY1基因进行研究,为更深入地了解其在杉木生长发育和环境适应性中调控作用提供参考。[方法]以杉木优良无性系‘洋020’的1年生苗作为试验材料,通过RT-PCR技术,完成了CRY1基因的克隆,并对其进行生物信息学和表达分析。[结果]ClCRY1基因CDS区全长2 109 bp,编码702个氨基酸（GenBank号为PP489217）,ClCRY1蛋白的分子式为C
Keyword ：
ClCRY1基因 ClCRY1基因 基因克隆 基因克隆 杉木 杉木 表达分析 表达分析 隐花色素 隐花色素
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| GB/T 7714 | 郭胜周 , 许祖元 , 陈樱 et al. 杉木隐花色素ClCRY1基因克隆及其表达分析 [J]. | 中南林业科技大学学报 , 2025 , 45 (03) : 138-147 . |
| MLA | 郭胜周 et al. "杉木隐花色素ClCRY1基因克隆及其表达分析" . | 中南林业科技大学学报 45 . 03 (2025) : 138-147 . |
| APA | 郭胜周 , 许祖元 , 陈樱 , 连怡然 , 曹光球 , 曹世江 . 杉木隐花色素ClCRY1基因克隆及其表达分析 . | 中南林业科技大学学报 , 2025 , 45 (03) , 138-147 . |
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