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学者姓名：吴松青
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Abstract ：
Pine wilt disease, caused by the pinewood nematode Bursaphelenchus xylophilus (Rhabditida: Aphelenchoididae), results in significant global economic and ecological impacts. Although the Cry13Aa1 toxin from Bacillus thuringiensis shows nematicidal activity, its mechanism of action against B. xylophilus remains unclear. This study aimed to identify and characterize the receptors for Cry13Aa1 in B. xylophilus. We cloned the cDNAs encoding an Snf7 domain-containing protein (BxSnf7) from B. xylophilus. Far-western blot analysis revealed a specific binding interaction between BxSnf7 and Cry13Aa1, showing a dissociation constant (Kd) of 20.8 +/- 4.2 nM. Interestingly, bioassay results indicated that silencing BxSnf7 increased the susceptibility of nematodes to Cry13Aa1 at higher concentrations, although the difference was not statistically significant. Besides, the combined application of BxSnf7 with Cry13Aa1 significantly enhanced nematicidal mortality (95.9 %) after 24 h of treatment, which higher than the expected mortality (42.8 %) (chi 2 = 16.118, P = 0.048), indicating that the exogenous BxSnf7 synergistically enhances the activity of Cry13Aa1 toxin. These findings identify BxSnf7 as a novel Cry13Aa1 binding protein and reveal a unique mechanism by which BxSnf7 synergistically enhances the activity of Cry13Aa1. However, BxSnf7 does not function as the primary receptor, and further research is needed to investigate its role in modulating nematode susceptibility to Cry13Aa1.
Keyword ：
Bursaphelenchus xylophilus Bursaphelenchus xylophilus Cry13Aa1 toxin Cry13Aa1 toxin Snf7-domain-containing protein Snf7-domain-containing protein Synergistic activity Synergistic activity
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| GB/T 7714 | Guo, Yajie , Huang, Lulu , Zhang, Hang et al. Identification of a Snf7-domain-containing protein that exhibits high affinity and synergistic activity for Cry13Aa1 toxin in Bursaphelenchus xylophilus [J]. | JOURNAL OF INVERTEBRATE PATHOLOGY , 2025 , 210 . |
| MLA | Guo, Yajie et al. "Identification of a Snf7-domain-containing protein that exhibits high affinity and synergistic activity for Cry13Aa1 toxin in Bursaphelenchus xylophilus" . | JOURNAL OF INVERTEBRATE PATHOLOGY 210 (2025) . |
| APA | Guo, Yajie , Huang, Lulu , Zhang, Hang , Li, Jun , Zhou, Yanyue , Sun, Yunzhu et al. Identification of a Snf7-domain-containing protein that exhibits high affinity and synergistic activity for Cry13Aa1 toxin in Bursaphelenchus xylophilus . | JOURNAL OF INVERTEBRATE PATHOLOGY , 2025 , 210 . |
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Aedes aegypti, known for transmitting viruses such as dengue, Zika and yellow fever, poses a significant public health threat. Conventional insecticides give rise to a range of issues, including ecological contamination and insect resistance. Hence, there is a pressing demand for environmentally-friendly, safer and more efficacious strategies for mosquito control. With the rapid advancement of the CRISPR/Cas9 system in gene function exploration and pest population control, substantial progress has been achieved in utilizing CRISPR/Cas9-based gene drive systems across various mosquito species. Only a few studies on gene drive technology have been conducted in Ae. aegypti. In this study, we constructed two complete drives for Ae. aegypti with different Cas9 promoters, each targeting kmo. Our drive based on PubCas9 had limited activity, but one with ExuCas9 exhibited super-Mendelian inheritance rates of approximately 60%. We observed low but detectable somatic activity of the drive and no evidence of maternally deposited Cas9. Germline resistance allele formation rates were similar to drive conversion rates, but most wild-type alleles in the germline remained uncut. Injections into the ExuCas9 drive line had 100% knockout efficiency among surviving offspring at three separate target genes. These results support the development and application of novel genetic pest control technologies aimed at combating Ae. aegypti.
Keyword ：
Aedes aegypti Aedes aegypti CRISPR/Cas9 CRISPR/Cas9 Gene drive Gene drive Homing drive Homing drive Mosquito control Mosquito control
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| GB/T 7714 | Yang, Xiaozhen , Xu, Xuejiao , Chen, Yixian et al. Assessment of drive efficiency and resistance allele formation of a homing gene drive in the mosquito Aedes aegypti [J]. | JOURNAL OF PEST SCIENCE , 2025 , 98 (2) : 899-911 . |
| MLA | Yang, Xiaozhen et al. "Assessment of drive efficiency and resistance allele formation of a homing gene drive in the mosquito Aedes aegypti" . | JOURNAL OF PEST SCIENCE 98 . 2 (2025) : 899-911 . |
| APA | Yang, Xiaozhen , Xu, Xuejiao , Chen, Yixian , Wei, Jiajia , Huang, Wanting , Wu, Songqing et al. Assessment of drive efficiency and resistance allele formation of a homing gene drive in the mosquito Aedes aegypti . | JOURNAL OF PEST SCIENCE , 2025 , 98 (2) , 899-911 . |
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Trichochrysea japana (Motschulsky, 1855) (Coleoptera: Chrysomelidae) is a horticultural pest with a widespread distribution. However, the complete mitochondrial genome (mitogenome) of this species has not been available . In this study, we sequenced and analyzed the first complete mitogenome of T. japana. The mitogenome is 15,681 bp in length (GenBank accession number: OR387477) and includes 13 typicalPCGs, 22 tRNAs, two rRNAs, and two control regions. Furthermore, phylogenetic analysis indicated that T. japana is closely related to Basilepta melanopus, B. fulvipes, and Colasposoma dauricum. The T. japana mitogenome data provides valuable insights into the genetic evolution of this species .
Keyword ：
mitogenome mitogenome phylogenetic analysis phylogenetic analysis Trichochrysea japana Trichochrysea japana
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| GB/T 7714 | Huang, Lulu , Zhang, Hang , Chen, Ying et al. The complete mitochondrial genome of Trichochrysea japana (Motschulsky, 1855) (Coleoptera: Chrysomeloidea) and its phylogenetic analyses [J]. | MITOCHONDRIAL DNA PART B-RESOURCES , 2025 , 10 (5) : 352-356 . |
| MLA | Huang, Lulu et al. "The complete mitochondrial genome of Trichochrysea japana (Motschulsky, 1855) (Coleoptera: Chrysomeloidea) and its phylogenetic analyses" . | MITOCHONDRIAL DNA PART B-RESOURCES 10 . 5 (2025) : 352-356 . |
| APA | Huang, Lulu , Zhang, Hang , Chen, Ying , Li, Xinquan , Wu, Jiankai , Wu, Songqing . The complete mitochondrial genome of Trichochrysea japana (Motschulsky, 1855) (Coleoptera: Chrysomeloidea) and its phylogenetic analyses . | MITOCHONDRIAL DNA PART B-RESOURCES , 2025 , 10 (5) , 352-356 . |
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In this paper, we describe a new stag beetle species from Yunnan, China, i.e., Neolucanus horizontalis Xin, Zhong & Qi, new species which exhibits significant structural differences in mandible, labial vestiture, protibia and male genitalia compared to related species. We also provide redescription of the male of N. fuscus Didier, 1926 and N. brochieri Bomans & Miyashita, 1997. Neolucans fuscus zhongguo Schenk, 2012 is synonymized with N. fuscus fuscus Didier, 1926. We also present a distribution map and an identification key for the telodont males of the N. fuscus group. Finally, we discuss the species group affiliation of N. baongocae Nguyen, 2013.
Keyword ：
Honghe Honghe morphology morphology Neolucanus Neolucanus new species new species redescription redescription stag beetle stag beetle taxonomy taxonomy
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| GB/T 7714 | Xin, Fei-yi , Zhong, Xiao-tian , Guo, Wen-chi et al. A taxonomic note on Chinese species of the Neolucanus fuscus group with the description of a new species (Coleoptera: Lucanidae: Lucaninae) [J]. | ZOOTAXA , 2025 , 5570 (2) : 344-360 . |
| MLA | Xin, Fei-yi et al. "A taxonomic note on Chinese species of the Neolucanus fuscus group with the description of a new species (Coleoptera: Lucanidae: Lucaninae)" . | ZOOTAXA 5570 . 2 (2025) : 344-360 . |
| APA | Xin, Fei-yi , Zhong, Xiao-tian , Guo, Wen-chi , Zhang, Yi-feng , Qi, Zhi-hao , Wu, Song-qing . A taxonomic note on Chinese species of the Neolucanus fuscus group with the description of a new species (Coleoptera: Lucanidae: Lucaninae) . | ZOOTAXA , 2025 , 5570 (2) , 344-360 . |
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The effectiveness of fluopyram suspension concentrate against pine wilt disease (PWD) is limited by spraying efficiency and water dependence. A traditional dust formulation with strong dispersibility can overcome these shortcomings. However, its efficacy against PWD remains uncertain. This study evaluated the translocation of fluopyram dust within tree tissues, soil and water degradation, and its effective control against PWD. Nursery tests showed effective prevention; field tests showed dust absorption and translocation into pine tissues. Thirty days following application, residual concentrations in soil were low at 0.09 mg kg-1; no detectable residues were found in water samples. Three years after applying fluopyram, its effectiveness increased to approximately 87%. Based on this study, fluopyram had a half-life of 346 d with persistence lasting up to three years. This provides valuable insight for managing PWD through dust applications.
Keyword ：
Dust formulation Dust formulation Efficacy Efficacy Fluopyram Fluopyram Persistence Persistence Pine wilt disease Pine wilt disease
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| GB/T 7714 | Guo, Yajie , Ma, Jiayi , Weng, Mingqing et al. Control efficacy and persistence of fluopyram dust against pine wilt disease [J]. | Journal of Forestry Research , 2025 , 36 (1) . |
| MLA | Guo, Yajie et al. "Control efficacy and persistence of fluopyram dust against pine wilt disease" . | Journal of Forestry Research 36 . 1 (2025) . |
| APA | Guo, Yajie , Ma, Jiayi , Weng, Mingqing , Carballar-Lejarazu, Rebeca , Jiao, Wenlong , Wu, Jieqin et al. Control efficacy and persistence of fluopyram dust against pine wilt disease . | Journal of Forestry Research , 2025 , 36 (1) . |
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The App6Aa2 toxin, derived from Bacillus thuringiensis, is nematicidal and highly toxic to Bursaphelenchus xylophilus. Receptors play a critical role in the mechanism of B. thuringiensis crystal toxin toxicity, yet the specific binding receptors for App6Aa2 in B. xylophilus have not been identified. This study identified a GPI-anchored protein, alpha-galactosidase (BxGal), in B. xylophilus as a potential binding protein. Western blotting and ELISA assays confirmed a high binding affinity between BxGal and App6Aa2 (Kd = 24.5 +/- 11.2 nM). Remarkably, combining App6Aa2 with the BxGal protein produced a synergistic effect, significantly increasing nematode mortality from 25 % to 81 % (P < 0.05), thereby enhancing the toxicity of App6Aa2 against B. xylophilus. Besides, RNAi silencing of BxGal in nematodes had no specific effect on App6Aa2 toxicity at high concentrations, while mortality increased slightly at lower concentrations. These findings indicate that BxGal is a high-affinity binding protein for App6Aa2; it does not function as the primary receptor and warrants further investigation into its role in modulating nematode susceptibility to App6Aa2.
Keyword ：
Affinity Affinity alpha-galactosidase alpha-galactosidase App6Aa2 toxin App6Aa2 toxin Bursaphelenchus xylophilus Bursaphelenchus xylophilus Synergistic activity Synergistic activity
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| GB/T 7714 | Guo, Yajie , Zhou, Yanyue , Li, Jun et al. Identification of an α-galactosidase with high affinity and synergistic activity against Bacillus thuringiensis App6Aa2 toxin in Bursaphelenchus xylophilus [J]. | PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY , 2025 , 208 . |
| MLA | Guo, Yajie et al. "Identification of an α-galactosidase with high affinity and synergistic activity against Bacillus thuringiensis App6Aa2 toxin in Bursaphelenchus xylophilus" . | PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY 208 (2025) . |
| APA | Guo, Yajie , Zhou, Yanyue , Li, Jun , Weng, Mingqing , Sun, Yunzhu , Wu, Songqing et al. Identification of an α-galactosidase with high affinity and synergistic activity against Bacillus thuringiensis App6Aa2 toxin in Bursaphelenchus xylophilus . | PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY , 2025 , 208 . |
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Pine wilt disease is a devastating disorder of pine trees, vectored and transmitted by Monochamus alternatus Hope. However, vector control strategies for this disease are hampered by the resistance mechanisms of the pathogen, which hinders the long-term survival of control agents in nature. Serratia marcescens, isolated from Pinus massoniana and the gut of M. alternatus, was selected as the carrier strain for engineered bacteria in this study owing to its symbiotic nature. To realize the possibility of the long-term survival of transgenic bacteria in the gut of M. alternatus and the wild, four sugar-inducible promoters were used to construct transgenic strains. The S. marcescens strains with sugar-inducible promoters exhibited higher fitness than the constitutive strain expressing the Cry3Aa-T toxin, while maintaining a growth curve similar to that of the wild-type strain. The conditional expression of the Cry3Aa-T toxin in S. marcescens was triggered by the presence of sugar, and the lactose-induced transgenic symbiotic strain significantly reduced the survival rate of M. alternatus. Thus, this strategy may enable the engineered bacteria to effectively colonize and spread in the wild, indicating a feasible and sustainable vector control approach against M. alternatus.
Keyword ：
Fitness cost Fitness cost Inducible promoter Inducible promoter Monochamus alternatus Monochamus alternatus Paratransgenesis Paratransgenesis Serratia marcescens Serratia marcescens Sugar Sugar
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| GB/T 7714 | Sheng, Liangjing , Ke, Sikai , Jiao, Wenlong et al. Sugar-inducible promoters mitigate the fitness cost of engineered Serratia marcescens in the control of Monochamus alternatus [J]. | MICROBIOLOGICAL RESEARCH , 2025 , 300 . |
| MLA | Sheng, Liangjing et al. "Sugar-inducible promoters mitigate the fitness cost of engineered Serratia marcescens in the control of Monochamus alternatus" . | MICROBIOLOGICAL RESEARCH 300 (2025) . |
| APA | Sheng, Liangjing , Ke, Sikai , Jiao, Wenlong , Li, Zibo , Zheng, Yunfeng , Tao, Silu et al. Sugar-inducible promoters mitigate the fitness cost of engineered Serratia marcescens in the control of Monochamus alternatus . | MICROBIOLOGICAL RESEARCH , 2025 , 300 . |
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The appressorium is a specialised infection structure formed by Beauveria bassiana during host invasion. This study used sulforaphane to regulate the formation rate of B. bassiana appressoria, evaluated the correlation between appressorium formation and fungal pathogenicity, and explored its impact on insect cuticular metabolism. The results showed that sulforaphane significantly modulated appressorium formation. Spore suspensions with varying appressorium formation rates were injected into Opisina arenosella and Bombyx mori larvae. As the appressorium formation rate increased, B. bassiana exhibited enhanced pathogenicity, leading to accelerated larval mortality. A significant positive correlation (p <= 0.05) was observed between appressorium formation and pathogenicity. LC-MS analysis revealed that, prior to appressorium development, larvae activated defence mechanisms involving secondary metabolites, hormone signalling, and toxin metabolism pathways. Following appressorium formation, 61 unique cuticular compounds were identified, along with activation of host lipid metabolism (notably glycerophospholipid degradation), programmed cell death pathways (ferroptosis, necroptosis), and enhanced energy metabolism via the citric acid cycle-collectively indicating disruption of the epidermal defence barrier. Overall, appressorium development by B. bassiana significantly reshapes the metabolic landscape of the larval cuticle, thereby enhancing fungal virulence. This study provides a theoretical foundation for understanding the pathogenic mechanisms of B. bassiana.
Keyword ：
appressorium appressorium Beauveria bassiana Beauveria bassiana insect epidermis insect epidermis metabolism metabolism pathogenicity pathogenicity
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| GB/T 7714 | Chen, Jiarui , Li, Wenzhe , Wu, Canxia et al. Analysis of the Effects of Beauveria bassiana Appressorium Formation on Insect Cuticle Metabolism Based on LC-MS [J]. | JOURNAL OF FUNGI , 2025 , 11 (8) . |
| MLA | Chen, Jiarui et al. "Analysis of the Effects of Beauveria bassiana Appressorium Formation on Insect Cuticle Metabolism Based on LC-MS" . | JOURNAL OF FUNGI 11 . 8 (2025) . |
| APA | Chen, Jiarui , Li, Wenzhe , Wu, Canxia , Wu, Songqing , Tong, Yinghua . Analysis of the Effects of Beauveria bassiana Appressorium Formation on Insect Cuticle Metabolism Based on LC-MS . | JOURNAL OF FUNGI , 2025 , 11 (8) . |
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Herbivore-induced plant volatiles (HIPVs) play a pivotal role in mediating tritrophic interactions between plants, herbivores, and their natural enemies. Paracarophenax alternatus, a parasitic mite targeting the egg stage of Monochamus alternatus, has emerged as a promising biocontrol agent. However, its ability to detect Pinus massoniana-derived HIPVs for host insect localization remains unclear. G-protein-coupled receptors (GPCRs) may play a role in mediating the perception of HIPVs and associated chemosensory signaling pathways in mites. In this study, a total of 85 GPCRs were identified from P. alternatus. All GPCRs exhibited conserved transmembrane domains and stage-specific expression patterns, with 21 receptors significantly upregulated in viviparous mites. Combined with two previously identified odorant receptors (ORs), six candidate chemosensory receptors were selected for molecular dynamics simulations to validate their binding stability with key volatile compounds. The results demonstrate that specific GPCRs likely facilitate HIPV detection in mites, enabling precise host localization within dynamic ecological niches. Our findings provide critical insights into the molecular basis of mite-host interactions and establish a framework for optimizing P. alternatus-based biocontrol strategies against pine wilt disease vectors.
Keyword ：
biocontrol biocontrol G-protein-coupled receptor G-protein-coupled receptor Monochamus alternatus Monochamus alternatus Paracarophenax alternatus Paracarophenax alternatus pine wilt disease pine wilt disease
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| GB/T 7714 | Lin, Ruiheng , Chu, Xu , Zhang, Yangming et al. Identification and Expression Analysis of G-Protein-Coupled Receptors Provide Insights into Functional and Mechanistic Responses to Herbivore-Induced Plant Volatiles of Paracarophenax alternatus [J]. | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES , 2025 , 26 (12) . |
| MLA | Lin, Ruiheng et al. "Identification and Expression Analysis of G-Protein-Coupled Receptors Provide Insights into Functional and Mechanistic Responses to Herbivore-Induced Plant Volatiles of Paracarophenax alternatus" . | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 26 . 12 (2025) . |
| APA | Lin, Ruiheng , Chu, Xu , Zhang, Yangming , Ke, Sikai , Zheng, Yunfeng , Yu, Wei et al. Identification and Expression Analysis of G-Protein-Coupled Receptors Provide Insights into Functional and Mechanistic Responses to Herbivore-Induced Plant Volatiles of Paracarophenax alternatus . | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES , 2025 , 26 (12) . |
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Pine wilt disease, a devastating disease severely impacting pine ecosystems, is caused by the pinewood nematode Bursaphelenchus xylophilus (Steiner & B & uuml;hrer, 1934) Nickle, 1970 (Nematoda: Parasitaphelenchidae). Controlling B. xylophilus is crucial for preventing and managing pine wilt disease. Recently discovered novel nematocidal lectins could provide more advantageous materials for utilizing genetically engineered bacteria to control this pathogen. Therefore, this study focuses on identifying novel nematocidal toxins within B. xylophilus lectins. Overall, we obtained twenty-one galectin, one L-type lectin (LTL), and three chitin-binding domain (CBD) genes by screening the B. xylophilus genome database; these genes were successfully expressed proteins. The bioassay results indicated that Bxgalectin2, Bxgalectin3, Bxgalectin4, Bxgalectin9, and BxLTL1 induced mortality rates exceeding 50% in B. xylophilus. Notably, Bxgalectin4 showed the strongest nematocidal activity, causing 88% mortality in the treated nematode population. The enzyme-linked immunosorbent assays further demonstrated that Bxgalectin3 (Kd = 8.992 nM) and Bxgalectin4 (Kd = 9.634 nM) had a higher binding affinity to GPI-anchored proteins from B. xylophilus. Additionally, Bxgalectin2 (Kd = 16.50 nM), Bxgalectin9 (Kd = 16.48 nM), and BxLTL1 (Kd = 24.34 nM) can bind to the GPI-anchored protein. This study reports, for the first time, that lectins endogenous to B. xylophilus exhibit nematocidal activity against their own species. These findings open up the possibility of using nematode lectins as potent control agents in the biological control of B. xylophilus.
Keyword ：
Bursaphelenchus xylophilus Bursaphelenchus xylophilus lectin lectin nematocidal activity nematocidal activity receptor binding receptor binding
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| GB/T 7714 | Wu, Songqing , Sun, Yunzhu , Li, Zibo et al. Nematocidal Activity and Intestinal Receptor-Binding Affinity of Endogenous Lectins in Bursaphelenchus xylophilus (Pinewood Nematode) [J]. | FORESTS , 2025 , 16 (7) . |
| MLA | Wu, Songqing et al. "Nematocidal Activity and Intestinal Receptor-Binding Affinity of Endogenous Lectins in Bursaphelenchus xylophilus (Pinewood Nematode)" . | FORESTS 16 . 7 (2025) . |
| APA | Wu, Songqing , Sun, Yunzhu , Li, Zibo , Li, Xinquan , Yu, Wei , Guo, Yajie . Nematocidal Activity and Intestinal Receptor-Binding Affinity of Endogenous Lectins in Bursaphelenchus xylophilus (Pinewood Nematode) . | FORESTS , 2025 , 16 (7) . |
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