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学者姓名：吴松青
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Abstract ：
Pine wilt disease, caused by the pinewood nematode Bursaphelenchus xylophilus (Rhabditida: Aphelenchoididae), results in significant global economic and ecological impacts. Although the Cry13Aa1 toxin from Bacillus thuringiensis shows nematicidal activity, its mechanism of action against B. xylophilus remains unclear. This study aimed to identify and characterize the receptors for Cry13Aa1 in B. xylophilus. We cloned the cDNAs encoding an Snf7 domain-containing protein (BxSnf7) from B. xylophilus. Far-western blot analysis revealed a specific binding interaction between BxSnf7 and Cry13Aa1, showing a dissociation constant (Kd) of 20.8 +/- 4.2 nM. Interestingly, bioassay results indicated that silencing BxSnf7 increased the susceptibility of nematodes to Cry13Aa1 at higher concentrations, although the difference was not statistically significant. Besides, the combined application of BxSnf7 with Cry13Aa1 significantly enhanced nematicidal mortality (95.9 %) after 24 h of treatment, which higher than the expected mortality (42.8 %) (chi 2 = 16.118, P = 0.048), indicating that the exogenous BxSnf7 synergistically enhances the activity of Cry13Aa1 toxin. These findings identify BxSnf7 as a novel Cry13Aa1 binding protein and reveal a unique mechanism by which BxSnf7 synergistically enhances the activity of Cry13Aa1. However, BxSnf7 does not function as the primary receptor, and further research is needed to investigate its role in modulating nematode susceptibility to Cry13Aa1.
Keyword ：
Bursaphelenchus xylophilus Bursaphelenchus xylophilus Cry13Aa1 toxin Cry13Aa1 toxin Snf7-domain-containing protein Snf7-domain-containing protein Synergistic activity Synergistic activity
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| GB/T 7714 | Guo, Yajie , Huang, Lulu , Zhang, Hang et al. Identification of a Snf7-domain-containing protein that exhibits high affinity and synergistic activity for Cry13Aa1 toxin in Bursaphelenchus xylophilus [J]. | JOURNAL OF INVERTEBRATE PATHOLOGY , 2025 , 210 . |
| MLA | Guo, Yajie et al. "Identification of a Snf7-domain-containing protein that exhibits high affinity and synergistic activity for Cry13Aa1 toxin in Bursaphelenchus xylophilus" . | JOURNAL OF INVERTEBRATE PATHOLOGY 210 (2025) . |
| APA | Guo, Yajie , Huang, Lulu , Zhang, Hang , Li, Jun , Zhou, Yanyue , Sun, Yunzhu et al. Identification of a Snf7-domain-containing protein that exhibits high affinity and synergistic activity for Cry13Aa1 toxin in Bursaphelenchus xylophilus . | JOURNAL OF INVERTEBRATE PATHOLOGY , 2025 , 210 . |
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In this paper, we describe a new stag beetle species from Yunnan, China, i.e., Neolucanus horizontalis Xin, Zhong & Qi, new species which exhibits significant structural differences in mandible, labial vestiture, protibia and male genitalia compared to related species. We also provide redescription of the male of N. fuscus Didier, 1926 and N. brochieri Bomans & Miyashita, 1997. Neolucans fuscus zhongguo Schenk, 2012 is synonymized with N. fuscus fuscus Didier, 1926. We also present a distribution map and an identification key for the telodont males of the N. fuscus group. Finally, we discuss the species group affiliation of N. baongocae Nguyen, 2013.
Keyword ：
Honghe Honghe morphology morphology Neolucanus Neolucanus new species new species redescription redescription stag beetle stag beetle taxonomy taxonomy
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| GB/T 7714 | Xin, Fei-yi , Zhong, Xiao-tian , Guo, Wen-chi et al. A taxonomic note on Chinese species of the Neolucanus fuscus group with the description of a new species (Coleoptera: Lucanidae: Lucaninae) [J]. | ZOOTAXA , 2025 , 5570 (2) : 344-360 . |
| MLA | Xin, Fei-yi et al. "A taxonomic note on Chinese species of the Neolucanus fuscus group with the description of a new species (Coleoptera: Lucanidae: Lucaninae)" . | ZOOTAXA 5570 . 2 (2025) : 344-360 . |
| APA | Xin, Fei-yi , Zhong, Xiao-tian , Guo, Wen-chi , Zhang, Yi-feng , Qi, Zhi-hao , Wu, Song-qing . A taxonomic note on Chinese species of the Neolucanus fuscus group with the description of a new species (Coleoptera: Lucanidae: Lucaninae) . | ZOOTAXA , 2025 , 5570 (2) , 344-360 . |
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为研究中华甲虫蒲螨Pyemotes zhonghuajia携带白僵菌孢子能力及所携带孢子活性和毒力，评估二者联合应用潜力，基于球孢白僵菌Beauveria bassiana BbFz-91菌株对中华甲虫蒲螨致病力低，采用白僵菌孢子喷粉法，用扫描电镜观察喷粉后12,24,48 h中华甲虫蒲螨体表携带孢子情况，用血球计数板和菌落计数法定量检测不同处理时间所携孢子的数量和活性，同时测定中华甲虫蒲螨所携孢子对松墨天牛的毒力。结果表明：中华甲虫蒲螨体表可携带白僵菌孢子，经喷粉后12 h，平均每头螨可携带1.54×10～5孢子，10头中华甲虫蒲螨携带并传播的白僵菌孢子平均在培养基上形成33个菌落，松墨天牛Monochamus alternatus幼虫接入中华甲虫蒲螨10 d后累计校正死亡率80.65%；随着喷粉后时间延长，携带孢子数量和形成的菌落数量逐渐减少，松墨天牛幼虫校正死亡率也逐渐降低。中华甲虫蒲螨喷粉后12 h对松墨天牛幼虫致死中时最短；喷粉后72 h，松墨天牛幼虫的致死中时显著延长。中华甲虫蒲螨具有主动传播白僵菌孢子能力，且散落孢子具有一定活性和毒力。林间应用中华甲虫蒲螨携带白僵菌联合防控松墨天牛具有可行性，结果为进一步开展林间应用技术研究奠定理论基础。
Keyword ：
中华甲虫蒲螨 中华甲虫蒲螨 孢子 孢子 松墨天牛 松墨天牛 毒力 毒力 活性 活性 球孢白僵菌 球孢白僵菌
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| GB/T 7714 | 伍鸿源 , 蔡守平 , 曾丽琼 et al. 中华甲虫蒲螨携带白僵菌孢子的能力及所携孢子活性和毒力 [J]. | 中国森林病虫 , 2025 , 44 (04) : 22-26 . |
| MLA | 伍鸿源 et al. "中华甲虫蒲螨携带白僵菌孢子的能力及所携孢子活性和毒力" . | 中国森林病虫 44 . 04 (2025) : 22-26 . |
| APA | 伍鸿源 , 蔡守平 , 曾丽琼 , 林浩宇 , 吴松青 , 徐云 . 中华甲虫蒲螨携带白僵菌孢子的能力及所携孢子活性和毒力 . | 中国森林病虫 , 2025 , 44 (04) , 22-26 . |
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Herbivore-induced plant volatiles (HIPVs) play a pivotal role in mediating tritrophic interactions between plants, herbivores, and their natural enemies. Paracarophenax alternatus, a parasitic mite targeting the egg stage of Monochamus alternatus, has emerged as a promising biocontrol agent. However, its ability to detect Pinus massoniana-derived HIPVs for host insect localization remains unclear. G-protein-coupled receptors (GPCRs) may play a role in mediating the perception of HIPVs and associated chemosensory signaling pathways in mites. In this study, a total of 85 GPCRs were identified from P. alternatus. All GPCRs exhibited conserved transmembrane domains and stage-specific expression patterns, with 21 receptors significantly upregulated in viviparous mites. Combined with two previously identified odorant receptors (ORs), six candidate chemosensory receptors were selected for molecular dynamics simulations to validate their binding stability with key volatile compounds. The results demonstrate that specific GPCRs likely facilitate HIPV detection in mites, enabling precise host localization within dynamic ecological niches. Our findings provide critical insights into the molecular basis of mite-host interactions and establish a framework for optimizing P. alternatus-based biocontrol strategies against pine wilt disease vectors.
Keyword ：
biocontrol biocontrol G-protein-coupled receptor G-protein-coupled receptor Monochamus alternatus Monochamus alternatus Paracarophenax alternatus Paracarophenax alternatus pine wilt disease pine wilt disease
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| GB/T 7714 | Lin, Ruiheng , Chu, Xu , Zhang, Yangming et al. Identification and Expression Analysis of G-Protein-Coupled Receptors Provide Insights into Functional and Mechanistic Responses to Herbivore-Induced Plant Volatiles of Paracarophenax alternatus [J]. | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES , 2025 , 26 (12) . |
| MLA | Lin, Ruiheng et al. "Identification and Expression Analysis of G-Protein-Coupled Receptors Provide Insights into Functional and Mechanistic Responses to Herbivore-Induced Plant Volatiles of Paracarophenax alternatus" . | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 26 . 12 (2025) . |
| APA | Lin, Ruiheng , Chu, Xu , Zhang, Yangming , Ke, Sikai , Zheng, Yunfeng , Yu, Wei et al. Identification and Expression Analysis of G-Protein-Coupled Receptors Provide Insights into Functional and Mechanistic Responses to Herbivore-Induced Plant Volatiles of Paracarophenax alternatus . | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES , 2025 , 26 (12) . |
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The appressorium is a specialised infection structure formed by Beauveria bassiana during host invasion. This study used sulforaphane to regulate the formation rate of B. bassiana appressoria, evaluated the correlation between appressorium formation and fungal pathogenicity, and explored its impact on insect cuticular metabolism. The results showed that sulforaphane significantly modulated appressorium formation. Spore suspensions with varying appressorium formation rates were injected into Opisina arenosella and Bombyx mori larvae. As the appressorium formation rate increased, B. bassiana exhibited enhanced pathogenicity, leading to accelerated larval mortality. A significant positive correlation (p <= 0.05) was observed between appressorium formation and pathogenicity. LC-MS analysis revealed that, prior to appressorium development, larvae activated defence mechanisms involving secondary metabolites, hormone signalling, and toxin metabolism pathways. Following appressorium formation, 61 unique cuticular compounds were identified, along with activation of host lipid metabolism (notably glycerophospholipid degradation), programmed cell death pathways (ferroptosis, necroptosis), and enhanced energy metabolism via the citric acid cycle-collectively indicating disruption of the epidermal defence barrier. Overall, appressorium development by B. bassiana significantly reshapes the metabolic landscape of the larval cuticle, thereby enhancing fungal virulence. This study provides a theoretical foundation for understanding the pathogenic mechanisms of B. bassiana.
Keyword ：
appressorium appressorium Beauveria bassiana Beauveria bassiana insect epidermis insect epidermis metabolism metabolism pathogenicity pathogenicity
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| GB/T 7714 | Chen, Jiarui , Li, Wenzhe , Wu, Canxia et al. Analysis of the Effects of Beauveria bassiana Appressorium Formation on Insect Cuticle Metabolism Based on LC-MS [J]. | JOURNAL OF FUNGI , 2025 , 11 (8) . |
| MLA | Chen, Jiarui et al. "Analysis of the Effects of Beauveria bassiana Appressorium Formation on Insect Cuticle Metabolism Based on LC-MS" . | JOURNAL OF FUNGI 11 . 8 (2025) . |
| APA | Chen, Jiarui , Li, Wenzhe , Wu, Canxia , Wu, Songqing , Tong, Yinghua . Analysis of the Effects of Beauveria bassiana Appressorium Formation on Insect Cuticle Metabolism Based on LC-MS . | JOURNAL OF FUNGI , 2025 , 11 (8) . |
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Pine wilt disease, a devastating disease severely impacting pine ecosystems, is caused by the pinewood nematode Bursaphelenchus xylophilus (Steiner & B & uuml;hrer, 1934) Nickle, 1970 (Nematoda: Parasitaphelenchidae). Controlling B. xylophilus is crucial for preventing and managing pine wilt disease. Recently discovered novel nematocidal lectins could provide more advantageous materials for utilizing genetically engineered bacteria to control this pathogen. Therefore, this study focuses on identifying novel nematocidal toxins within B. xylophilus lectins. Overall, we obtained twenty-one galectin, one L-type lectin (LTL), and three chitin-binding domain (CBD) genes by screening the B. xylophilus genome database; these genes were successfully expressed proteins. The bioassay results indicated that Bxgalectin2, Bxgalectin3, Bxgalectin4, Bxgalectin9, and BxLTL1 induced mortality rates exceeding 50% in B. xylophilus. Notably, Bxgalectin4 showed the strongest nematocidal activity, causing 88% mortality in the treated nematode population. The enzyme-linked immunosorbent assays further demonstrated that Bxgalectin3 (Kd = 8.992 nM) and Bxgalectin4 (Kd = 9.634 nM) had a higher binding affinity to GPI-anchored proteins from B. xylophilus. Additionally, Bxgalectin2 (Kd = 16.50 nM), Bxgalectin9 (Kd = 16.48 nM), and BxLTL1 (Kd = 24.34 nM) can bind to the GPI-anchored protein. This study reports, for the first time, that lectins endogenous to B. xylophilus exhibit nematocidal activity against their own species. These findings open up the possibility of using nematode lectins as potent control agents in the biological control of B. xylophilus.
Keyword ：
Bursaphelenchus xylophilus Bursaphelenchus xylophilus lectin lectin nematocidal activity nematocidal activity receptor binding receptor binding
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| GB/T 7714 | Wu, Songqing , Sun, Yunzhu , Li, Zibo et al. Nematocidal Activity and Intestinal Receptor-Binding Affinity of Endogenous Lectins in Bursaphelenchus xylophilus (Pinewood Nematode) [J]. | FORESTS , 2025 , 16 (7) . |
| MLA | Wu, Songqing et al. "Nematocidal Activity and Intestinal Receptor-Binding Affinity of Endogenous Lectins in Bursaphelenchus xylophilus (Pinewood Nematode)" . | FORESTS 16 . 7 (2025) . |
| APA | Wu, Songqing , Sun, Yunzhu , Li, Zibo , Li, Xinquan , Yu, Wei , Guo, Yajie . Nematocidal Activity and Intestinal Receptor-Binding Affinity of Endogenous Lectins in Bursaphelenchus xylophilus (Pinewood Nematode) . | FORESTS , 2025 , 16 (7) . |
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Chitin synthase (CHS) plays a key role in chitin synthesis. CHS1 is ubiquitous in insects, and some studies have found that the RNA interference with CHS1 can hinder three types of molting processes (larva-larva, larva-pupa and pupa-adult). In the present study, the CHS1 of Monochamus alternatus was identified and characterized by a bioinformatics analysis. The developmental stage-specific expression of the MalCHS1 (Monochamus alternatus CHS1) gene was obtained by a RT-qPCR, and the corresponding dsRNA was designed for functional verification. The RNA interference experiment was conducted using the microinjection method, and the injection site was selected from the abdominal segments of fifth-instar larvae. The results showed that after silencing the CHS1 gene, the larvae of M. alternatus showed morphological abnormalities, such as the softening of the body wall, a transparent abdomen and the swelling of somites, indicating that MalCHS1 mediates the molting, growth and development of M. alternatus. RNAi-mediated MalCHS1 gene silencing may become a promising new biological pesticide that can provide a new target gene for pest control.
Keyword ：
chitin synthase 1 chitin synthase 1 growth and development growth and development molting molting Monochamus alternatus Monochamus alternatus RNA interference RNA interference
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| GB/T 7714 | Ye, Wanlin , Li, Tong , Weng, Mingqing et al. RNAi-Mediated Silencing of Chitin Synthase 1 (CHS1) Disrupts Molting and Growth in Monochamus alternatus [J]. | FORESTS , 2025 , 16 (6) . |
| MLA | Ye, Wanlin et al. "RNAi-Mediated Silencing of Chitin Synthase 1 (CHS1) Disrupts Molting and Growth in Monochamus alternatus" . | FORESTS 16 . 6 (2025) . |
| APA | Ye, Wanlin , Li, Tong , Weng, Mingqing , Guo, Wenchi , Xin, Feiyi , Yu, Wei et al. RNAi-Mediated Silencing of Chitin Synthase 1 (CHS1) Disrupts Molting and Growth in Monochamus alternatus . | FORESTS , 2025 , 16 (6) . |
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Trichochrysea japana (Motschulsky, 1855) (Coleoptera: Chrysomelidae) is a horticultural pest with a widespread distribution. However, the complete mitochondrial genome (mitogenome) of this species has not been available . In this study, we sequenced and analyzed the first complete mitogenome of T. japana. The mitogenome is 15,681 bp in length (GenBank accession number: OR387477) and includes 13 typicalPCGs, 22 tRNAs, two rRNAs, and two control regions. Furthermore, phylogenetic analysis indicated that T. japana is closely related to Basilepta melanopus, B. fulvipes, and Colasposoma dauricum. The T. japana mitogenome data provides valuable insights into the genetic evolution of this species .
Keyword ：
mitogenome mitogenome phylogenetic analysis phylogenetic analysis Trichochrysea japana Trichochrysea japana
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| GB/T 7714 | Huang, Lulu , Zhang, Hang , Chen, Ying et al. The complete mitochondrial genome of Trichochrysea japana (Motschulsky, 1855) (Coleoptera: Chrysomeloidea) and its phylogenetic analyses [J]. | MITOCHONDRIAL DNA PART B-RESOURCES , 2025 , 10 (5) : 352-356 . |
| MLA | Huang, Lulu et al. "The complete mitochondrial genome of Trichochrysea japana (Motschulsky, 1855) (Coleoptera: Chrysomeloidea) and its phylogenetic analyses" . | MITOCHONDRIAL DNA PART B-RESOURCES 10 . 5 (2025) : 352-356 . |
| APA | Huang, Lulu , Zhang, Hang , Chen, Ying , Li, Xinquan , Wu, Jiankai , Wu, Songqing . The complete mitochondrial genome of Trichochrysea japana (Motschulsky, 1855) (Coleoptera: Chrysomeloidea) and its phylogenetic analyses . | MITOCHONDRIAL DNA PART B-RESOURCES , 2025 , 10 (5) , 352-356 . |
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[Objective] Paracarophenax alternatus is a newly identified parasitic mite exhibiting parasitic efficacy against Monochamus alternatus eggs. This study aims to investigate the effects of environmental factors on the parasitism efficiency of P. alternatus, to evaluate its potential in biological control programs, to optimize artificial rearing systems, and to refine field application strategies.[Method] An indoor feeding experiment under controlled laboratory conditions was conducted to assess the impacts of M. alternatus egg age (0-4 days post-oviposition), mite-to-egg ratios (1∶1-8∶1), temperature (17-33 ℃), relative humidity (RH: 60%-95%), and photoperiod (24L, 12L∶12D, 24D) on the parasitism rates of P. alternatus. A predictive model was developed using random forest regression analysis to quantify parasitism rates under varying environmental conditions. [Result]1) Under the conditions (mite-to-egg ratio 5∶1, 25°C, 85% RH, 12L∶12D), the parasitism rate of P. alternatus on newly laid eggs of M. alternatus was the highest, reaching 74.44%, significantly exceeded the parasitism rate of 44.44% on 4-day-old eggs of M. alternatus P2 = 0.83), and predicted the maximum parasitism rates (>90%) under conditions of freshly laid eggs, 5∶1 mite-to-egg ratio, 20-31 ℃, RH >93%, and 24D photoperiod.[Conclusion]Egg age, temperature, humidity, photoperiod, and mite-to-egg ratio significantly influence the parasitism efficiency of P. alternatus on the eggs of M. alternatu. For optimal biological control or mass rearing, conditions should include mite-to-egg ratios ≥5∶1, temperatures of 21-29 ℃, RH ≥90%, and complete darkness. These findings provide critical insights for optimizing mass-rearing protocols and field deployment strategies to enhance the efficacy of P. alternatus in suppressing M. alternatus populations. © 2025 Chinese Society of Forestry. All rights reserved.
Keyword ：
Enterprise software Enterprise software Logistic regression Logistic regression Mobile applications Mobile applications
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| GB/T 7714 | Li, Junnan , Fu, Yu , Chen, Runkai et al. Research on Paracarophenax alternatus Xu et Zhang Ⅳ-Effects of Environmental Factors on Parasitism Rate [J]. | Scientia Silvae Sinicae , 2025 , 61 (5) : 161-170 . |
| MLA | Li, Junnan et al. "Research on Paracarophenax alternatus Xu et Zhang Ⅳ-Effects of Environmental Factors on Parasitism Rate" . | Scientia Silvae Sinicae 61 . 5 (2025) : 161-170 . |
| APA | Li, Junnan , Fu, Yu , Chen, Runkai , Xu, Yun , Cai, Mengling , Luo, Shengjie et al. Research on Paracarophenax alternatus Xu et Zhang Ⅳ-Effects of Environmental Factors on Parasitism Rate . | Scientia Silvae Sinicae , 2025 , 61 (5) , 161-170 . |
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The effectiveness of fluopyram suspension concentrate against pine wilt disease (PWD) is limited by spraying efficiency and water dependence. A traditional dust formulation with strong dispersibility can overcome these shortcomings. However, its efficacy against PWD remains uncertain. This study evaluated the translocation of fluopyram dust within tree tissues, soil and water degradation, and its effective control against PWD. Nursery tests showed effective prevention; field tests showed dust absorption and translocation into pine tissues. Thirty days following application, residual concentrations in soil were low at 0.09 mg kg-1; no detectable residues were found in water samples. Three years after applying fluopyram, its effectiveness increased to approximately 87%. Based on this study, fluopyram had a half-life of 346 d with persistence lasting up to three years. This provides valuable insight for managing PWD through dust applications.
Keyword ：
Dust formulation Dust formulation Efficacy Efficacy Fluopyram Fluopyram Persistence Persistence Pine wilt disease Pine wilt disease
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| GB/T 7714 | Guo, Yajie , Ma, Jiayi , Weng, Mingqing et al. Control efficacy and persistence of fluopyram dust against pine wilt disease [J]. | Journal of Forestry Research , 2025 , 36 (1) . |
| MLA | Guo, Yajie et al. "Control efficacy and persistence of fluopyram dust against pine wilt disease" . | Journal of Forestry Research 36 . 1 (2025) . |
| APA | Guo, Yajie , Ma, Jiayi , Weng, Mingqing , Carballar-Lejarazu, Rebeca , Jiao, Wenlong , Wu, Jieqin et al. Control efficacy and persistence of fluopyram dust against pine wilt disease . | Journal of Forestry Research , 2025 , 36 (1) . |
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