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学者姓名:杨志坚
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本发明提供了一种结合普通油茶果实性状的高产分子标记及育种方法。属于基因育种技术领域。本发明开发出了ISSR815、ISSR835和ISSR858这3个与普通油茶杂交果实性状紧密连锁的高产分子标记,获得815‑11、835‑12、858‑2和858‑3这4个基因位点,结合杂交果实的果高和种子鲜重这2个性状,在自然群体中19个杂交家系中选择普通油茶高产优良家系和植株,选择效率均达75%,正确率为100%,按照该方法获得普通油茶高产家系,产量高,平均单位冠幅面产量为2.86kg/m2,远远高于平均单位冠幅面产量1.2kg/m2的国家行业标准;在每一个杂交世代中,2年内就可成功筛选出高产品种,相比于现有技术的十几二十年,极大缩短了育种周期,解决了现有油茶杂交育种周期长、稳定性差和效率低的问题。
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| GB/T 7714 | 冯金玲 , 杨志坚 , 郑金佳 . 一种结合普通油茶果实性状的高产分子标记及育种方法 : CN202410284255.9[P]. | 2024-03-13 . |
| MLA | 冯金玲 等. "一种结合普通油茶果实性状的高产分子标记及育种方法" : CN202410284255.9. | 2024-03-13 . |
| APA | 冯金玲 , 杨志坚 , 郑金佳 . 一种结合普通油茶果实性状的高产分子标记及育种方法 : CN202410284255.9. | 2024-03-13 . |
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Background C. Oleifera is among the world's largest four woody plants known for their edible oil production, yet the contribution rate of improved varieties is less than 20%. The species traditional breeding is lengthy cycle (20-30 years), occupation of land resources, high labor cost, and low accuracy and efficiency, which can be enhanced by molecular marker-assisted selection. However, the lack of high-quality molecular markers hinders the species genetic analysis and molecular breeding. Results Through quantitative traits characterization, genetic diversity assessment, and association studies, we generated a selection population with wide genetic diversity, and identified five excellent high-yield parental combinations associated with four reliable high-yield ISSR markers. Early selection criteria were determined based on kernel fresh weight and cultivated 1-year seedling height, aided by the identification of these 4 ISSR markers. Specific assignment of selected individuals as paternal and maternal parents was made to capitalize on their unique attributes. Conclusions Our results indicated that molecular markers-assisted breeding can effectively shorten, enhance selection accuracy and efficiency and facilitate the development of a new breeding system for C. oleifera.
Keyword :
Camellia oleifera Camellia oleifera High-yield early identification High-yield early identification ISSR marker development ISSR marker development Marker-association analysis Marker-association analysis Molecular marker assisted-breeding Molecular marker assisted-breeding
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| GB/T 7714 | Zheng, Jinjia , Su, Haiqi , Pu, Shaosheng et al. High-yield hybrid breeding of Camellia oleifolia based on ISSR molecular markers [J]. | BMC PLANT BIOLOGY , 2024 , 24 (1) . |
| MLA | Zheng, Jinjia et al. "High-yield hybrid breeding of Camellia oleifolia based on ISSR molecular markers" . | BMC PLANT BIOLOGY 24 . 1 (2024) . |
| APA | Zheng, Jinjia , Su, Haiqi , Pu, Shaosheng , Chen, Hui , El-Kassaby, Yousry A. , Yang, Zhijian et al. High-yield hybrid breeding of Camellia oleifolia based on ISSR molecular markers . | BMC PLANT BIOLOGY , 2024 , 24 (1) . |
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本发明公开了南方水土流失区构建稳定马尾松森林群落的方法,涉及森林培育方法领域。包括以下步骤:1)在流失班中构建稳定马尾松森林群落;2)在10年马尾松林中构建稳定马尾松森林群落;3)在20年马尾松林中构建稳定马尾松森林群落;4)在30年马尾松林中构建稳定马尾松森林群落;5)在40年马尾松林中构建稳定马尾松森林群落。本发明能够根据不同阶段的马尾松林分的特征和和亚热带常绿阔叶林演替规律,在南方水土流失区有效地构建稳定马尾松森林群落,恢复水土保持功能。
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| GB/T 7714 | 杨志坚 , 冯金玲 , 成艺宇 . 南方水土流失区构建稳定马尾松森林群落的方法 : CN202410142677.2[P]. | 2024-02-01 . |
| MLA | 杨志坚 et al. "南方水土流失区构建稳定马尾松森林群落的方法" : CN202410142677.2. | 2024-02-01 . |
| APA | 杨志坚 , 冯金玲 , 成艺宇 . 南方水土流失区构建稳定马尾松森林群落的方法 : CN202410142677.2. | 2024-02-01 . |
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本发明公开了一种利用CRISPR‑Cas13a检测甘薯SPVD病毒病的方法,主要内容包括LwaCas13a基因的表达与蛋白纯化、guide RNA的获得、LwaCas13a‑sgRNA复合物组装、甘薯SPVD病毒病检测。主要原理是,构建LwaCas13a‑sgRNA复合物,利用其中的gRNA特异识别病毒RNA后,启动Cas13a剪切RNA的功能,释放荧光信号,经检测荧光信号,可快速检测低浓度的病毒RNA。该方法是继指示植物嫁接法、PCR法和ELISA三种甘薯病毒病检测方法之外的一种新方法,具有检测时间短、不需要逆转录、快速且高效的检测方法,检测的病毒浓度下限与PCR方法相当。
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| GB/T 7714 | 林世强 , 陈选阳 , 杨志坚 et al. 一种利用CRISPR-Cas13a检测甘薯SPVD病毒病的方法 : CN202411144569.5[P]. | 2024-08-20 . |
| MLA | 林世强 et al. "一种利用CRISPR-Cas13a检测甘薯SPVD病毒病的方法" : CN202411144569.5. | 2024-08-20 . |
| APA | 林世强 , 陈选阳 , 杨志坚 , 黄碧芳 , 许明 . 一种利用CRISPR-Cas13a检测甘薯SPVD病毒病的方法 : CN202411144569.5. | 2024-08-20 . |
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本发明公开了一种结合油茶苗期性状的高产杂交选育方法。属于林木遗传育种技术领域。本发明通过构建油茶多世代杂交群体,开发了高产亲本选择、杂交、苗木培育、高产优良杂交苗木家系选择,高产优良植株选择的方法,建立了一种结合油茶苗期性状的高产育种体系,利用该方法选育的高产家系,选择效率89%,选择正确率为100%,高产优良植株平均单位冠幅面积产量大于3.02Kg/m2,解决了现有油茶杂交育种周期长和效率低的问题。
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| GB/T 7714 | 冯金玲 , 杨志坚 . 一种结合油茶苗期性状的高产杂交选育方法 : CN202410285014.6[P]. | 2024-03-13 . |
| MLA | 冯金玲 et al. "一种结合油茶苗期性状的高产杂交选育方法" : CN202410285014.6. | 2024-03-13 . |
| APA | 冯金玲 , 杨志坚 . 一种结合油茶苗期性状的高产杂交选育方法 : CN202410285014.6. | 2024-03-13 . |
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本发明公开了一种基于物联网的提高油茶产量的水分管理方法。属于林业栽培技术领域。将物联网土壤含水量传感器安装于油茶树西南方向滴水线上,深度25~35cm,运用物联网土壤环境监测仪器实时监测油茶林地土壤含水量,每30分钟记录1次数据,当监测到油茶林地土壤水分达到所设定阀值,向监测系统中心或管理人手机报警,再通过系统或人控制开启水阀,进行定量滴灌,并自动计录每次滴灌水量和次数。通过物联网技术进行土壤含水量的实时监测,并结合油茶树生长周期特点和对水分的需求特性,通过远程控制滴灌系统及时适量地补充油茶林地水分,便捷高效,大大促进了油茶的生长和产量,与无水分管理比较,茶油单株产量提高了100~154%。
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| GB/T 7714 | 冯金玲 , 杨志坚 , 黄蓝明 et al. 一种基于物联网的提高油茶产量的水分管理方法 : CN202410284146.7[P]. | 2024-03-13 . |
| MLA | 冯金玲 et al. "一种基于物联网的提高油茶产量的水分管理方法" : CN202410284146.7. | 2024-03-13 . |
| APA | 冯金玲 , 杨志坚 , 黄蓝明 , 陈来贺 , 邓青 . 一种基于物联网的提高油茶产量的水分管理方法 : CN202410284146.7. | 2024-03-13 . |
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本发明公开了一种结合普通油茶果实性状的油茶高产杂交选育方法,涉及油茶高产选育技术领域,过程包括:选择高产亲本、杂交,获得杂交群体家系;通过回归方程Y=10468.848+436.015X1+2345.035X2预测杂交群体各家系的单位冠幅面积产量,其中Y代表杂交家系的单位冠幅面积产量,X1代表杂交家系果实的单果重,X2代表杂交家系果实的单果籽粒数;按照预测的家系油茶单位冠幅面积产量高于预测杂交群体平均值30%的标准筛选高产杂交家系种子;在高产杂交家系种子中,按照籽粒重高于家系平均值30%的标准筛选高产杂交家系种子;对高产杂交家系种子预处理,并把高产杂交家系种子培育为一年生苗木,再按照苗高高于家系平均值30%的标准筛选高产杂交优良苗木。本发明建立了一种结合普通油茶果实性状的高产杂交育种技术体系,并利用该技术选育的高产家系,选择效率大于60%,选择正确率为100%,高产优良植株平均单位冠幅面积产量大于2.51kg/m2,解决了现有油茶杂交育种周期长和效率低的问题。
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| GB/T 7714 | 冯金玲 , 杨志坚 . 一种结合普通油茶果实性状的油茶高产杂交选育方法 : CN202410284663.4[P]. | 2024-03-13 . |
| MLA | 冯金玲 et al. "一种结合普通油茶果实性状的油茶高产杂交选育方法" : CN202410284663.4. | 2024-03-13 . |
| APA | 冯金玲 , 杨志坚 . 一种结合普通油茶果实性状的油茶高产杂交选育方法 : CN202410284663.4. | 2024-03-13 . |
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本实用新型涉及一种操作简便的烤薯机,包括内部设有保温腔和烤制腔的箱体,保温腔与烤制腔之间通过甘薯输出口连通,烤制腔的内部上端设有若干个由旋转机构驱动旋转的烤筒,烤筒的底部设有由电动机构驱动上下摆转的密封盖;烤制腔的顶部设有若干个甘薯入口,若干个甘薯入口与若干个烤筒的位置相对应;烤制腔的内部下端设有用于呈接从若干个烤筒底部落下的甘薯的输送带,输送带的输出端与甘薯输出口相衔接,以将甘薯输送至保温腔。设计合理,甘薯受热均匀,烤制时间短,烤制结束后通过输送带将甘薯输送至保温腔,无需手动夹取,有效避免造成烫伤,操作方便;同时整体体积小,可放置在柜台上,能更好的适用于小型店铺,方便商家售卖,减少人与人之间的接触。
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| GB/T 7714 | 覃晓晖 , 杨志坚 , 陈选阳 et al. 一种操作简便的烤薯机 : CN202321349851.8[P]. | 2023-05-31 . |
| MLA | 覃晓晖 et al. "一种操作简便的烤薯机" : CN202321349851.8. | 2023-05-31 . |
| APA | 覃晓晖 , 杨志坚 , 陈选阳 , 陈义鑫 , 王楠 , 孙宜新 et al. 一种操作简便的烤薯机 : CN202321349851.8. | 2023-05-31 . |
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本发明公开了一种富含花色苷的紫薯薯圆,其是以紫心甘薯新品种金薯20为主要原料,通过添加木薯粉、太白粉及甜叶菊制备获得。本发明从164个甘薯种质资源中筛选出适合制作薯圆的紫心甘薯品种金薯20,利用其为原料制备薯圆,并结合太白粉的使用,可在保持薯圆的口感及外观的同时,大大降低木薯粉的用量,从而提高薯圆食用的安全性及健康性。
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| GB/T 7714 | 杨志坚 , 陈选阳 , 陈凌华 . 一种富含花色苷的紫薯薯圆 : CN202310709392.8[P]. | 2023-06-15 . |
| MLA | 杨志坚 et al. "一种富含花色苷的紫薯薯圆" : CN202310709392.8. | 2023-06-15 . |
| APA | 杨志坚 , 陈选阳 , 陈凌华 . 一种富含花色苷的紫薯薯圆 : CN202310709392.8. | 2023-06-15 . |
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BackgroundMost of Camellia oleifera forests have low fruit yield and poor oil quality that are largely associated with soil fertility. Soil physical and chemical properties interact with each other affecting soil fertility and C. oleifera growing under different soil conditions produced different yield and oil composition. Three main soil types were studied, and redundancy, correlation, and double-screening stepwise regression analysis were used for exploring the relationships between C. oleifera nutrients uptake and soil physical and chemical properties, shedding light on the transport law of nutrient elements from root, leaves, and kernel, and affecting the regulation of fruit yield and oil composition.ResultsIn the present study, available soil elements content of C. oleifera forest were mainly regulated by water content, pH value, and total N, P and Fe contents. Seven elements (N, P, K, Mg, Cu, Mn and C) were key for kernel's growth and development, with N, P, K, Cu and Mn contents determining 74.0% the yield traits. The transport characteristics of these nutrients from root, leaves to the kernel had synergistic and antagonistic effects. Increasing oil production and unsaturated fatty acid content can be accomplished in two ways: one through increasing N, P, Mg, and Zn contents of leaves by applying corresponding N, P, Mg, Zn foliar fertilizers, while the other through maintaining proper soil moisture content by applying Zn fertilizer in the surface layer and Mg and Ca fertilizer in deep gully.ConclusionSoil type controlled nutrient absorption by soil pH, water content and total N, P and Fe content. There were synergistic and antagonistic effects on the inter-organ transport of nutrient elements, ultimately affecting N, P, K, Cu and Mn contents in kernel, which determined the yield and oil composition of C. oleifera.
Keyword :
Camellia oleifera Camellia oleifera Key nutrient elements Key nutrient elements Oil composition regulation Oil composition regulation Organ Organ Soil conditions Soil conditions Yield regulation Yield regulation
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| GB/T 7714 | Chen, Yu , Zheng, Jinjia , Yang, Zhijian et al. Role of soil nutrient elements transport on Camellia oleifera yield under different soil types [J]. | BMC PLANT BIOLOGY , 2023 , 23 (1) . |
| MLA | Chen, Yu et al. "Role of soil nutrient elements transport on Camellia oleifera yield under different soil types" . | BMC PLANT BIOLOGY 23 . 1 (2023) . |
| APA | Chen, Yu , Zheng, Jinjia , Yang, Zhijian , Xu, Chenhao , Liao, Penghui , Pu, Shaosheng et al. Role of soil nutrient elements transport on Camellia oleifera yield under different soil types . | BMC PLANT BIOLOGY , 2023 , 23 (1) . |
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