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学者姓名:邹双全
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Abstract :
Fruit features are crucial for plant propagation, population growth, biodiversity preservation, and evolutionary survival. However, the synergistic regulatory mechanisms underlying the development of fruit traits such as color, shape and duration are unclear. Euscaphis japonica, whose fruits have a red-winged pericarp and persist for a long period of time, is an important ornamental plant in eastern Asia. In this study, we present a complete proteome spanning multiple time points and the phosphoproteome landscape of E. japonica fruit during the maturation and ripening phases. Quantitative evaluation via proteome and phosphoproteome analysis revealed three distinct phases that are consistent with the fruit maturation and ripening stages on a longitudinal time scale. The two-way proteomics analysis revealed functionally important biological events, including anthocyanin accumulation and phytohormone and light signal transduction. Importantly, our integrated analysis, along with experimental validation and phytohormone treatments, suggested that alterations in EjPHYBS21/S37 and EjPHOT1S394/S429 phosphorylation may lead to auxin accumulation and the inhibition of ethylene biosynthesis, thereby initiating the development of long-duration, red butterfly-shaped E. japonica fruit. Our study reveals a mechanism of E. japonica fruit formation that highlights plant adaptive strategies that potentially evolved through interactions with frugivores.
Keyword :
Auxin accumulation Auxin accumulation Euscaphis japonica Euscaphis japonica Fruit development Fruit development Light signaling Light signaling Phosphoproteomics Phosphoproteomics Proteomics Proteomics
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| GB/T 7714 | Liu, Bobin , Yang, Qixin , Xin, Gui-Liang et al. A comprehensive proteomic map revealing the regulation of the development of long-duration, red butterfly-shaped fruit in Euscaphis japonica [J]. | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES , 2025 , 292 . |
| MLA | Liu, Bobin et al. "A comprehensive proteomic map revealing the regulation of the development of long-duration, red butterfly-shaped fruit in Euscaphis japonica" . | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 292 (2025) . |
| APA | Liu, Bobin , Yang, Qixin , Xin, Gui-Liang , Wang, Xiaqin , Zhang, Li , He, Dongmei et al. A comprehensive proteomic map revealing the regulation of the development of long-duration, red butterfly-shaped fruit in Euscaphis japonica . | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES , 2025 , 292 . |
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以栀子YABBY基因家族为研究对象,利用TBtools、MEGA、ITOL等软件对栀子YABBY基因家族成员进行鉴定与生物信息学分析,包括栀子YABBY成员鉴定、理化性质、染色体定位、蛋白保守基序、系统进化树、顺式作用元件、共线性和栀子花不同时期表达量进行分析,以期为栀子YABBY基因鉴定及分子功能提供参考依据。结果表明:栀子YABBY基因家族共有5个成员,分布在Chr2、Chr5、Chr9和Chr11染色体上,其结构域保守,均为低亲水性蛋白;顺式元件分析表明,栀子YABBY基因家族含有光响应、非生物胁迫和激素类响应元件;表达模式分析表明,栀子GjYABBY1~GjYABBY3在花不同时期不同部位都有表达,GjYABBY3在雄蕊中持续高表达,有特异性表达;GjYABBY4在萼片发育的Ⅰ和Ⅲ期有高表达,而在不同时期花瓣、花管、雄蕊、雌蕊上均不表达。综上所述,YABBY基因在栀子花和果实生长过程中起重要调控作用。
Keyword :
YABBY基因 YABBY基因 栀子 栀子 生物信息学 生物信息学
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| GB/T 7714 | 林宇清 , 朱渊铭 , 陈丝雨 et al. 栀子YABBY基因家族鉴定及功能分析 [J]. | 北方园艺 , 2025 , 8 (05) : 1-8 . |
| MLA | 林宇清 et al. "栀子YABBY基因家族鉴定及功能分析" . | 北方园艺 8 . 05 (2025) : 1-8 . |
| APA | 林宇清 , 朱渊铭 , 陈丝雨 , 陈德星 , 张琼艺 , 邹双全 . 栀子YABBY基因家族鉴定及功能分析 . | 北方园艺 , 2025 , 8 (05) , 1-8 . |
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【目的】为进一步探究ARF基因在圆齿野鸦椿生长与发育过程中的作用机制提供科学依据。【方法】通过圆齿野鸦椿全基因组,利用生物信息学,鉴定其ARF基因家族成员,分析EkARF基因的理化性质、染色体定位、进化关系、基因结构、所包含的结构域以及顺式作用元件。探究EkARF基因在不同组织中的表达模式并进行功能预测。【结果】共鉴定出29个EkARF基因(EkARF1~EkARF29),分为4个亚族;除LG02染色体外,其余染色体上均有分布。29个ARF基因的氨基酸数量介于432~1110个,相对分子质量在48 159.66~122 724.62 u,均为亲水性蛋白,其亚细胞定位预测均在细胞核内。29个EkARF蛋白均含有DBD、Auxin_resp结构域,其中21个含有CTD结构域,其基因启动子顺式作用元件与植物光响应、激素的调节、防御机制和生长发育密切相关。EkARF基因在不同组织中表达存在差异,EkARF10在种子中高表达,可能参与种子发育调控;EkARF5随着果实的成熟表达量逐渐降低,可能在果实发育过程中起重要调控作用;EkARF16、EkARF20随着果实发育表达量上调,尤其是EkARF20在果实脱落期呈高表达,与果实成熟变红后仍挂果超130 d不脱落的现象一致,可能是果实发育的关键调控基因。【结论】本研究揭示了EkARF基因在圆齿野鸦椿不同组织部位中的表达模式,为阐明EkARF基因在圆齿野鸦椿生长发育过程中的功能提供理论基础。
Keyword :
ARF基因家族 ARF基因家族 圆齿野鸦椿 圆齿野鸦椿 生物信息学 生物信息学 表达分析 表达分析
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| GB/T 7714 | 陈丝雨 , 张培兰 , 朱渊铭 et al. 圆齿野鸦椿ARF基因家族鉴定和功能分析 [J]. | 西南农业学报 , 2025 , 38 (01) : 31-40 . |
| MLA | 陈丝雨 et al. "圆齿野鸦椿ARF基因家族鉴定和功能分析" . | 西南农业学报 38 . 01 (2025) : 31-40 . |
| APA | 陈丝雨 , 张培兰 , 朱渊铭 , 林宇清 , 陈德星 , 冯翯 et al. 圆齿野鸦椿ARF基因家族鉴定和功能分析 . | 西南农业学报 , 2025 , 38 (01) , 31-40 . |
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Anthocyanins, crucial water-soluble pigments in plants, determine coloration in floral and fruit tissues, while fulfilling essential physiological roles in terms of plant growth, development, and stress adaptation. The biosynthesis of anthocyanins is transcriptionally regulated by WRKY factors, one of the largest plant-specific transcription factor families. Euscaphis japonica is an East Asian species, prized for its exceptionally persistent butterfly-shaped fruits that undergo pericarp dehiscence, overturning, and a color transition to scarlet red. This species represents an ideal system for studying anthocyanin regulation. However, the mechanisms by which WRKY transcription factors orchestrate anthocyanin accumulation during this process remain unknown. In this study, we identified 87 WRKY genes (EjaWRKYs) from the E. japonica genome. Phylogenetic analysis was used to classify these genes into three primary groups, with five subgroups, revealing conserved gene structures and motif compositions, supported by collinearity and comparative synteny analyses. Crucially, ten EjaWRKYs exhibited peak expression during the mature fruit stages, showing positive correlations with key anthocyanin biosynthesis genes. Functional validation through the use of transient transactivation assays in Nicotiana benthamiana confirmed that the five selected EjaWRKYs bind W-box elements and strongly activate reporter gene expression. Our results reveal EjaWRKYs' regulation of anthocyanin accumulation in E. japonica fruit, provide the first comprehensive WRKY family characterization of this species, and establish a foundation for manipulating ornamental traits in horticultural breeding.
Keyword :
anthocyanins anthocyanins Euscaphis japonica Euscaphis japonica genome-wide genome-wide WRKY WRKY
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| GB/T 7714 | Liu, Bobin , Wang, Qingying , He, Dongmei et al. Genome-Wide Identification and Co-Expression Analysis of WRKY Genes Unveil Their Role in Regulating Anthocyanin Accumulation During Euscaphis japonica Fruit Maturation [J]. | BIOLOGY-BASEL , 2025 , 14 (8) . |
| MLA | Liu, Bobin et al. "Genome-Wide Identification and Co-Expression Analysis of WRKY Genes Unveil Their Role in Regulating Anthocyanin Accumulation During Euscaphis japonica Fruit Maturation" . | BIOLOGY-BASEL 14 . 8 (2025) . |
| APA | Liu, Bobin , Wang, Qingying , He, Dongmei , Wang, Xiaqin , Xin, Guiliang , Zou, Xiaoxing et al. Genome-Wide Identification and Co-Expression Analysis of WRKY Genes Unveil Their Role in Regulating Anthocyanin Accumulation During Euscaphis japonica Fruit Maturation . | BIOLOGY-BASEL , 2025 , 14 (8) . |
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为明确栀子TCP基因家族及盐胁迫响应,利用生物信息学手段对栀子TCP基因家族进行鉴定、分析和盐胁迫表达试验分析。结果表明,在栀子中共鉴定出16个TCP基因,分为PCF、CYC和CIN共3个亚家族。保守基序、基因结构和理化性质表明,同亚家族基因保守基序相似,不同亚家族的基因保守基序差异较大,16个栀子TCP基因均为亲水蛋白。染色体定位表明,16个栀子TCP基因不均匀地分布在8条染色体上,且包含多个片断段复制事件。顺式作用元件分析发现,栀子TCP基因中含有多种与植物生长发育、应激反应及激素调节相关的作用元件,以胁迫响应元件MYB和MYc最多;盐胁迫表达量显示,栀子TCP基因均能响应盐胁迫,部分基因在重度盐胁迫特异性表达,推测GjTCP13、GjTCP12和GjTCP6可能为栀子耐盐调控的关键基因。综上所述,栀子TCP基因家族成员能够积极响应盐胁迫,增强栀子的耐盐抗性。
Keyword :
TCP基因家族 TCP基因家族 栀子 栀子 生物信息学 生物信息学 盐胁迫 盐胁迫
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| GB/T 7714 | 林宇清 , 朱渊铭 , 陈丝雨 et al. 栀子TCP基因家族鉴定及响应盐胁迫表达模式分析 [J]. | 江苏农业科学 , 2025 , 53 (04) : 126-132 . |
| MLA | 林宇清 et al. "栀子TCP基因家族鉴定及响应盐胁迫表达模式分析" . | 江苏农业科学 53 . 04 (2025) : 126-132 . |
| APA | 林宇清 , 朱渊铭 , 陈丝雨 , 陈德星 , 张琼艺 , 陈淑颖 et al. 栀子TCP基因家族鉴定及响应盐胁迫表达模式分析 . | 江苏农业科学 , 2025 , 53 (04) , 126-132 . |
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本发明涉及一种延长芳樟新稍观赏期并提升芳樟醇含量的方法,其是在芳樟油用林投产后,在每年植株地上部分采收后及春季抽稍前各喷施0.50‑1.85 mmol·L‑1茉莉酸甲酯一次;或是在芳樟观赏林或休闲林或康养林在春季抽稍前喷施0.50‑1.85 mmol·L‑1茉莉酸甲酯二次;发明方法可以促进抽梢和展叶,延长芳樟新稍观赏期,提升成熟叶片芳樟醇相对含量。喷施用量每次每株50‑100 mL。芳樟植株的枝叶产量增长率在13%-23%,芳樟枝叶芳樟醇的相对含量提高11%‑22.3%,抽梢时间提前5‑10 d,新梢转色观赏时间延长5‑10 d;不但可以提高芳樟枝叶产量,也极大提高了新梢转色观赏期,可以吸引更新人康养、休闲提升了效益。
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| GB/T 7714 | 蔡宗鑫 , 丘菊兰 , 彭金彬 et al. 一种延长芳樟新稍观赏期并提升芳樟醇含量的方法 : CN202510112318.7[P]. | 2025-01-24 . |
| MLA | 蔡宗鑫 et al. "一种延长芳樟新稍观赏期并提升芳樟醇含量的方法" : CN202510112318.7. | 2025-01-24 . |
| APA | 蔡宗鑫 , 丘菊兰 , 彭金彬 , 吴建凯 , 郑鸿飞 , 唐勇 et al. 一种延长芳樟新稍观赏期并提升芳樟醇含量的方法 : CN202510112318.7. | 2025-01-24 . |
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【目的】探究不同水氮耦合处理对金线莲生长和品质的影响,为确定金线莲栽培的适宜水氮耦合方案提供参考。【方法】以大叶金线莲为试材,采取裂区设计,设置3种施氮量(N1:3.3 g/m~2;N2:6.6 g/m~2;N3:9.9g/m~2)和4种灌水量(W1:(45±5)%θ_f;W2:(55±5)%θ_f;W3:(65±5)%θ_f;W4:(75±5)%θ_f;其中θ_f为田间持水量)组合处理,以不施肥、不灌溉为对照(CK),栽培5个月后,测定13个处理金线莲的生长指标(保存率、株高、单株叶片数、叶长和叶宽、茎节数、节长、根长、根数、单株鲜质量及干质量、折干率)、生理指标(总叶绿素、可溶性蛋白、可溶性糖含量和过氧化物酶、超氧化物歧化酶活性)和品质指标(总黄酮含量及总多糖、金线莲苷、总生物碱和总氨基酸质量分数),分析灌水量和施氮量对金线莲生长及活性成分的影响。【结果】(1)与CK相比,除N1W1、N3W1处理外,其余处理均可以提高金线莲单株鲜质量、干质量、折干率、茎节数、节长和株高,其中N2W3处理金线莲的单株鲜质量、茎节数、节长、株高均达最大值,较CK分别提高了35.67%,33.18%,16.14%和35.45%。在30~60 d的较短栽培时期内,金线莲栽培的保存率在各处理之间差异不明显,但随着栽培时间延长,N1、N2条件下的水氮耦合优势逐渐显现。与CK相比,各处理金线莲的单株叶片数、叶长、叶宽、根长和根数无显著差异。(2)与CK相比,除N2W1处理外,其余处理均可以提高金线莲可溶性糖含量、SOD活性和总叶绿素含量;除N1W4和N3W4处理外,其余处理均可以降低金线莲的POD活性。与CK相比,各处理对金线莲可溶性蛋白含量有显著影响,同一施氮水平下,W1条件下的可溶性蛋白含量显著高于其他处理。(3)与CK相比,各处理对金线莲总氨基酸和总多糖质量分数均有显著影响,其中N2W3处理的总氨基酸质量分数最高,为9.18%;N1W3处理的总多糖质量分数最高,为45.08%。在W2、W3条件下,金线莲的总黄酮含量和金线莲苷质量分数随施氮量的增加均呈先增后减趋势,施氮效果均表现为N2>N3>N1,且均以N2W3处理最高,分别为25.04 mg/g和19.36%。(4)熵权系数法分析结果显示,12种肥水组合中,耦合作用效果排名前5的处理为N2W3>N2W2>N3W1>N2W4>N3W2。【结论】适宜的水氮耦合处理有利于金线莲生长及活性...
Keyword :
水氮耦合 水氮耦合 活性成分 活性成分 生理特性 生理特性 金线莲 金线莲
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| GB/T 7714 | 汪其双 , 陈泽明 , 林协全 et al. 水氮耦合对金线莲生长和品质的影响 [J]. | 西北农林科技大学学报(自然科学版) , 2024 , (08) : 1-12 . |
| MLA | 汪其双 et al. "水氮耦合对金线莲生长和品质的影响" . | 西北农林科技大学学报(自然科学版) 08 (2024) : 1-12 . |
| APA | 汪其双 , 陈泽明 , 林协全 , 覃兴化 , 黄思琦 , 邹双全 et al. 水氮耦合对金线莲生长和品质的影响 . | 西北农林科技大学学报(自然科学版) , 2024 , (08) , 1-12 . |
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目的:研究腐殖质土添加不同腐熟程度杉木屑对金线莲生长与活性成分的影响。方法:以金线莲组培苗为材料,设置100%腐殖质土(处理A)、40%腐熟杉木屑+60%腐殖质土(处理B)、40%未腐熟杉木屑+60%腐殖质土(处理C)3个处理,测定金线莲生长指标以及活性成分含量,分析腐殖质土中添加不同腐熟程度杉木屑对杉木下仿野生栽培金线莲生长及活性成分的影响。结果:种植在处理B中的金线莲幼苗保存率最高,达到了84.80%;幼苗茎粗、叶片数、叶片长、叶片宽、根长的增长量均高于其余2个处理;总黄酮与生物碱含量分别比处理A与处理C高出1.17%、25.63%和3.55%、11.34%。结论:腐殖质土中添加杉木屑可提高金线莲保存率,促进金线莲生长,其中60%腐殖土+40%腐熟杉木屑栽培效果最佳,值得进一步推广。
Keyword :
杉木屑 杉木屑 活性成分 活性成分 腐熟度 腐熟度 金线莲 金线莲
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| GB/T 7714 | 钱承文 , 罗栩辉 , 林协全 et al. 腐殖质土添加杉木屑对金线莲生长及活性成分的影响 [J]. | 中国野生植物资源 , 2024 , 43 (03) : 39-43 . |
| MLA | 钱承文 et al. "腐殖质土添加杉木屑对金线莲生长及活性成分的影响" . | 中国野生植物资源 43 . 03 (2024) : 39-43 . |
| APA | 钱承文 , 罗栩辉 , 林协全 , 刘锦航 , 邹双全 , 邹小兴 . 腐殖质土添加杉木屑对金线莲生长及活性成分的影响 . | 中国野生植物资源 , 2024 , 43 (03) , 39-43 . |
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Objective: In this study, we aimed to investigate the effects of facile combinations of biochar and N, P, and K fertilisers on the growth and physiological characteristics of Lithocarpus litseifolius seedlings, and to optimise the biochar/NPK ratio of Lithocarpus litseifolius. Method: A four-factor three-level orthogonal method was used to conduct a field experiment using 2-year-old Lithocarpus litseifolius. Nine fertiliser treatments and one control treatment (CK, i.e., no fertiliser) were established in a completely randomised block group with six replications of ten treatments. The plants were planted in October 2020 and harvested in November 2021, and fertiliser was applied once in April 2021 and once in July 2021. Results: Rational application could effectively promote the growth of Lithocarpus litseifolius, and T4(C2N1P2K3) produced the highest increase in plant height growth (17.03 cm), diameter growth (5.47 mm), soluble sugar (94.60 mg/g), soluble protein (4.59 mg/g), and phlorizin (old leaf: 16.00%; tender leaf: 15.13%); T8(C3N2P1K3) resulted in the highest chlorophyll a content (1.46 mg/g), chlorophyll b content (0.62 mg/g), and total chlorophyll content (2.08 mg/g), and T1(C1N1P1K1) resulted in the highest contents of starch (11.60 mg/g) and trilobatin (old leaf: 0.29%; tender leaf: 2.28%). The indicators corresponding to the above three treatments were significantly higher than those under the other treatments (p < 0.05). The results as analysed by the affiliation function method show that the highest mean value of the affiliation function was 0.645 after T4(C2N1P2K3) treatment. Conclusions: The combination of biochar and nitrogen, phosphorus, and potassium fertilisers can effectively increase the biomass and active components of Lithocarpus litseifolius while reducing the amount of chemical fertiliser applied. A comprehensive analysis of the results showed that the T4 treatment (biochar: 20 g/plant; urea: 10 g/plant; superphosphate: 9 g/plant; potassium chloride: 12 g/plant) resulted in the highest comprehensive score, with the highest increase in plant height growth, ground diameter growth, root-crown ratio, soluble sugar, soluble protein, and phlorizin, as well as other indicators.
Keyword :
biochar biochar formulated fertilisation formulated fertilisation growth indices growth indices Lithocarpus litseifolius Lithocarpus litseifolius physiological indices physiological indices
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| GB/T 7714 | Ye, Zelin , Zhang, Hang , Lin, Xiequan et al. Effect of Biochar Using N, P, and K Fertilisers on Growth and Quality of Lithocarpus litseifolius [J]. | AGRONOMY-BASEL , 2024 , 14 (4) . |
| MLA | Ye, Zelin et al. "Effect of Biochar Using N, P, and K Fertilisers on Growth and Quality of Lithocarpus litseifolius" . | AGRONOMY-BASEL 14 . 4 (2024) . |
| APA | Ye, Zelin , Zhang, Hang , Lin, Xiequan , Huang, Siqi , Zou, Shuangquan , Zou, Xiaoxing . Effect of Biochar Using N, P, and K Fertilisers on Growth and Quality of Lithocarpus litseifolius . | AGRONOMY-BASEL , 2024 , 14 (4) . |
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为研究樟叶总木脂素的最佳回流提取工艺及抑菌活性,以樟叶为研究对象,测定其芝麻素和9-羟基芝麻素含量,通过单因素联合Box-Behnken响应面法对樟叶总木脂素回流提取工艺进行优化,并采用带毒平板法对总木脂素的抑菌活性进行评价。结果表明,回流提取最佳工艺条件为提取温度86℃、料液比1∶8.39(g·mL~(-1))、提取时间43 min、提取溶剂为无水乙醇,在此条件下木脂素提取率达0.380 4%。各因素对提取率的影响表现为提取温度>提取时间>料液比。抑菌活性试验表明,木脂素含量为2 mg·mL~(-1)时对链孢粘帚霉菌(Gliocladium catenulatum)、苹果黑腐皮壳菌(Valsa mali Miyabe et Yamada)、西瓜尖孢镰孢菌(Fusarium oxysporumf)和瓜果腐霉菌(Pythium aphanidermatum)的抑菌效果最佳,抑菌率均达到50%以上;有效中质量浓度(EC_(50))分别为1.238、1.280、1.486和0.419 mg·mL~(-1)。以上研究结果为樟叶开发利用及植物源农药研究及开发提供了理论参考。
Keyword :
响应面法 响应面法 抑菌活性 抑菌活性 木脂素 木脂素 樟 樟 毒力 毒力
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| GB/T 7714 | 张岚雄 , 郑威 , 陈源桉 et al. 樟叶总木脂素提取工艺及其抑菌活性研究 [J]. | 中国农业科技导报 , 2024 , 26 (05) : 138-147 . |
| MLA | 张岚雄 et al. "樟叶总木脂素提取工艺及其抑菌活性研究" . | 中国农业科技导报 26 . 05 (2024) : 138-147 . |
| APA | 张岚雄 , 郑威 , 陈源桉 , 沈婧 , 邹双全 , 伍建榕 et al. 樟叶总木脂素提取工艺及其抑菌活性研究 . | 中国农业科技导报 , 2024 , 26 (05) , 138-147 . |
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