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学者姓名：张清华
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Abstract ：
Auxin response factors (ARFs) are pivotal transcription factors that regulate plant growth, development, and stress responses. Yet, the genomic characteristics and functions of ARFs in Phoebe bournei remain undefined. In this study, 25 PbARF genes were identified for the first time across the entire genome of P. bournei. Phylogenetic analysis categorized these genes into five subfamilies, with members of each subfamily displaying similar conserved motifs and gene structures. Notably, Classes III and V contained the largest number of members. Collinearity analysis suggested that segmental duplication events were the primary drivers of PbARF gene family expansion. Structural analysis revealed that all PbARF genes possess a conserved B3 binding domain and an auxin response element, while additional motifs varied among different classes. Promoter cis-acting element analysis revealed that PbARF genes are extensively involved in hormonal responses-particularly to abscisic acid and jasmonic acid and abiotic stresses-as well as abiotic stresses, including heat, drought, light, and dark. Tissue-specific expression analysis showed that PbARF25, PbARF23, PbARF19, PbARF22, and PbARF20 genes (class III), and PbARF18 and PbARF11 genes (class V) consistently exhibited high expression levels in the five tissues. In addition, five representative PbARF genes were analyzed using qRT-PCR. The results demonstrated significant differences in the expression of PbARF genes under various abiotic stress conditions (drought, salt stress, light, and dark), indicating their important roles in stress response. This study laid a foundation for elucidating the molecular evolution mechanism of ARF genes in P. bournei and for determining the candidate genes for stress-resistance breeding.
Keyword ：
ARF ARF expansion expansion gene family gene family Phoebe bournei Phoebe bournei stress response stress response
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| GB/T 7714 | Zheng, Kehui , Feng, Yizhuo , Liu, Ronglin et al. Bioinformatics Analysis Reveals the Evolutionary Characteristics of the Phoebe bournei ARF Gene Family and Its Expression Patterns in Stress Adaptation [J]. | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES , 2025 , 26 (8) . |
| MLA | Zheng, Kehui et al. "Bioinformatics Analysis Reveals the Evolutionary Characteristics of the Phoebe bournei ARF Gene Family and Its Expression Patterns in Stress Adaptation" . | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 26 . 8 (2025) . |
| APA | Zheng, Kehui , Feng, Yizhuo , Liu, Ronglin , Zhang, Yanlin , Fan, Dunjin , Zhong, Kai et al. Bioinformatics Analysis Reveals the Evolutionary Characteristics of the Phoebe bournei ARF Gene Family and Its Expression Patterns in Stress Adaptation . | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES , 2025 , 26 (8) . |
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Fujian cypress is a precious and endangered coniferous species. Since 2019, Fujian cypress trees in Anxi County, Fujian Province have suffered a novel lethal disease. This disease initially manifests as twig wilting of one or several branches, which then extends to all branches, leading to tree death within one or two months. The branch blight is associated with stem bark rot and discoloration of the cambial tissue. Pathogenicity tests showed that five fungal isolates could induce stem bark rot and cambial discoloration on branches in vitro, and their representative isolate Fhb-2 caused branch blight and sapling death symptoms that were similar to those observed in naturally infected trees. By combining morphological characteristics with phylogenetic analysis based on partial sequences of translation elongation factor 1-alpha (tef1-alpha), internal transcribed spacer regions of ribosomal deoxyribonucleic acid, and beta-tubulin, the five pathogen isolates were identified as Neofusicoccum parvum. Additionally, the sensitivity of isolate Fhb-2 to seven fungicides was assessed. The assessment revealed that thiophanate-methyl exhibited the strongest antifungal effect on N. parvum, with an EC50 value of 0.011 mu g ml-1, followed by flusilazole with an EC50 value of 0.057 mu g ml-1. As far as we are aware, this is the first report of N. parvum causing branch blight disease of Fujian cypress, and triazole and benzimidazoles are proposed for disease control.
Keyword ：
branch blight branch blight Fujian cypress Fujian cypress fungicide fungicide Neofusicoccum parvum Neofusicoccum parvum
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| GB/T 7714 | Zhao, Wenbao , Xue, Lili , Lu, Yongfeng et al. Identification, pathogenicity, and fungicide screening of Neofusicoccum parvum, causal agent of a new branch blight disease on Fujian cypress (Fokienia hodginsii) [J]. | FORESTRY , 2025 , 98 (5) : 776-785 . |
| MLA | Zhao, Wenbao et al. "Identification, pathogenicity, and fungicide screening of Neofusicoccum parvum, causal agent of a new branch blight disease on Fujian cypress (Fokienia hodginsii)" . | FORESTRY 98 . 5 (2025) : 776-785 . |
| APA | Zhao, Wenbao , Xue, Lili , Lu, Yongfeng , Gong, Weiyong , Wang, Guobin , Li, Yuanshun et al. Identification, pathogenicity, and fungicide screening of Neofusicoccum parvum, causal agent of a new branch blight disease on Fujian cypress (Fokienia hodginsii) . | FORESTRY , 2025 , 98 (5) , 776-785 . |
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Anthracnose, caused by Colletotrichum gloeosporioides (Cog), severely constrains the Cymbidium ensifolium industry. To investigate the molecular underpinnings of resistance and the host-pathogen interaction strategies between C. ensifolium and Cog, we employed transcriptomics and metabolomics to compare the post-infection responses of the Cog-resistant (RV) and Cog-susceptible (SV) C. ensifolium varieties. Our integrated analysis reveals that resistance to Cog in C. ensifolium is partially mediated by the targeted accumulation of phenylpropane pathway metabolites, especially those involved in flavone and flavonol biosynthesis. Metabolites including rutin, lonicerin, nicotiflorin, apiin, and coniferin exhibited highly significant accumulation in the RV. The massive accumulation of various flavonoids in the SV was consistent with the gene expression trends in the phenylpropanoid pathway, a pattern indicative of an antioxidant stress response driven by stress reprogramming. A similar phenomenon was also observed in the core reactive oxygen species (ROS) scavenging pathway, glutathione metabolism. This ultimately results in two distinct outcomes: a potent, antifungal defense reprogramming in the RV versus an antioxidant-focused stress reprogramming in the SV. The observed trade-offs between antifungal and antioxidant activities in these varieties provide novel insights into the multilevel regulatory networks governing plant-pathogen interactions. Our study illuminates this sophisticated defense strategy of C. ensifolium against Cog, identifying core metabolites and pathways that now serve as a guide for targeted resistance breeding programs.
Keyword ：
Antioxidant Antioxidant Colletotrichum gloeosporioides Colletotrichum gloeosporioides Cymbidium ensifolium Cymbidium ensifolium Flavonoids Flavonoids Phenylpropanoid pathway Phenylpropanoid pathway Resistance strategy Resistance strategy
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| GB/T 7714 | Li, Peng , Lan, Linying , Xiong, Longwei et al. Integrated transcriptome and metabolome analysis reveal a reprogramming of phenylpropanoid pathway and antioxidant activity in Cymbidium ensifolium resistance to anthracnose [J]. | JOURNAL OF PLANT PHYSIOLOGY , 2025 , 315 . |
| MLA | Li, Peng et al. "Integrated transcriptome and metabolome analysis reveal a reprogramming of phenylpropanoid pathway and antioxidant activity in Cymbidium ensifolium resistance to anthracnose" . | JOURNAL OF PLANT PHYSIOLOGY 315 (2025) . |
| APA | Li, Peng , Lan, Linying , Xiong, Longwei , Tian, Yang , Wu, Gangmu , Peng, Donghui et al. Integrated transcriptome and metabolome analysis reveal a reprogramming of phenylpropanoid pathway and antioxidant activity in Cymbidium ensifolium resistance to anthracnose . | JOURNAL OF PLANT PHYSIOLOGY , 2025 , 315 . |
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Simple Summary The molecular pathogenesis of Bipolaris fujianensis, a novel fungal pathogen causing shoot blight in Chinese fir (Cunninghamia lanceolata), remains unclear. Through comparative transcriptomics, we analyzed its gene expression during infection. Key findings include the identification of differentially expressed genes involved in transport, hydrolysis, carbon metabolism, and biosynthesis of secondary metabolites. Notably, we predicted the involvement of cytochrome P450 and a major facilitator superfamily (MFS) transporter in the production and transport of the diterpenoid toxin ophiobolin F. These insights advance the understanding of B. fujianensis virulence and may facilitate the development of control strategies against this disease.Abstract Bipolaris fujianensis is a novel pathogenic species causing Chinese fir (Cunninghamia lanceolata) shoot blight (CFSB), first discovered in Nanping City, Fujian Province. However, its molecular pathogenic mechanisms remain largely unknown. Elucidating theses mechanisms has the potential of aiding future developments in disease management and resistance breeding in Chinese fir. In this regard, we examined the expression pattern of B. fujianensis grown on PDA (BGPDA), and during infection of Chinese fir at 24 h (BGCF-E), 48 h (BGCF-M), and 5 d (BGCF-L) post inoculation. Comparative transcriptome analysis identified 4133 differentially expressed genes (DEGs), including 1778 upregulated and 2355 downregulated in BGCF compared with BGPDA. During the infection process, Gene ontology (GO) enrichment analysis indicated that transporters and hydrolases in the molecular function categories play essential roles. Kyoto encyclopedia of genes and genomes (KEGG) analyses showed glycolysis/gluconeogenesis, carbon metabolism, and secondary metabolite biosynthesis were the major enriched pathways. Furthermore, this pathogen could produce diterpenoid toxin ophiobolin F, with cytochrome P450 and MFS transport proteins likely involved in its biosynthesis and transport predicted by RT-qPCR.
Keyword ：
Bipolaris fujianensis Bipolaris fujianensis Chinese fir shoot blight Chinese fir shoot blight pathogenicity pathogenicity RNA-seq RNA-seq
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| GB/T 7714 | Lin, Bin , Yang, Peiwen , Luo, Ruifeng et al. Transcriptome Analysis of the Pathogenic Mechanism of the Novel Pathogenic Fungus Bipolaris fujianensis in Chinese Fir (Cunninghamia lanceolata) Shoot Blight [J]. | BIOLOGY-BASEL , 2025 , 14 (11) . |
| MLA | Lin, Bin et al. "Transcriptome Analysis of the Pathogenic Mechanism of the Novel Pathogenic Fungus Bipolaris fujianensis in Chinese Fir (Cunninghamia lanceolata) Shoot Blight" . | BIOLOGY-BASEL 14 . 11 (2025) . |
| APA | Lin, Bin , Yang, Peiwen , Luo, Ruifeng , Lu, Ying , Li, Zhe , Shang, Menglan et al. Transcriptome Analysis of the Pathogenic Mechanism of the Novel Pathogenic Fungus Bipolaris fujianensis in Chinese Fir (Cunninghamia lanceolata) Shoot Blight . | BIOLOGY-BASEL , 2025 , 14 (11) . |
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Fusarium wilt in Cymbidium ensifolium, caused by Fusarium oxysporum, is highly contagious and poses a severe hazard. It significantly reduces the ornamental value of C. ensifolium and causes substantial economic losses in agricultural production. Nucleotide-binding site-leucine-rich repeat (NBS-LRR) genes are key regulatory factors in plant disease resistance responses, playing vital roles in defending against pathogen invasions. In our study, we conducted a comprehensive analysis of the NBS-LRR gene family in the genome of Cymbidium ensifolium. Phylogenetic analysis identified a total of 31 NBS-LRR genes encoding NB-ARC proteins, which were categorized into five classes (CNL, CN, NL, N, RNL) based on their protein structural domains. These genes were found to be unevenly distributed across eight chromosomes. Physicochemical analysis revealed significant variances in molecular weight and sequence length among the family members. Subcellular localization results indicated that most genes primarily reside in the cytoplasm and cell membrane, suggesting that the primary sites of disease resistance responses may be the cell membrane and cyto-plasm. Furthermore, noticeable disparities were observed in gene structures and conserved motifs among different categories of family genes. Promoter analysis indicated that cis-regulatory elements are mainly associated with plant stress, jasmonic acid, gibberellin, and other development-related factors, suggesting that CeNBS-LRR genes mainly resist external stress through hormones such as abscisic acid and jasmonic acid. We characterized twenty-seven CeNBS-LRR gene expression patterns of healthy C. ensifolium at different periods after Fusarium wilt infection, and found that those genes exhibit a temporospatial expression pattern, and that their expression is also responsive to Fusarium wilt infection. By analyzing the expression pattern via transcriptome and qRT-PCR, we speculated that JL006442 and JL014305 may play key roles in resisting Fusarium wilt. This study lays the groundwork and holds considerable significance as a reference for identifying disease-resistant genes and facilitating genetic breeding in C. ensifolium.
Keyword ：
Cymbidium ensifolium Cymbidium ensifolium Fusarium wilt Fusarium wilt NBS-LRR NBS-LRR resistance expression resistance expression R gene R gene
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| GB/T 7714 | Yan, Lu , Su, Bin-Xian , Li, Jin-Jin et al. Genome-Wide Identification of Nucleotide-Binding Site-Leucine-Rich Repeat Gene Family in Cymbidium ensifolium and Expression Profiles in Response to Fusarium Wilt Infection [J]. | HORTICULTURAE , 2024 , 10 (6) . |
| MLA | Yan, Lu et al. "Genome-Wide Identification of Nucleotide-Binding Site-Leucine-Rich Repeat Gene Family in Cymbidium ensifolium and Expression Profiles in Response to Fusarium Wilt Infection" . | HORTICULTURAE 10 . 6 (2024) . |
| APA | Yan, Lu , Su, Bin-Xian , Li, Jin-Jin , Li, Yu-Yan , Chen, Shu-Yi , Feng, Cai-Yun et al. Genome-Wide Identification of Nucleotide-Binding Site-Leucine-Rich Repeat Gene Family in Cymbidium ensifolium and Expression Profiles in Response to Fusarium Wilt Infection . | HORTICULTURAE , 2024 , 10 (6) . |
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福建平脐蠕孢菌Bipolaris fujianensis是杉木苗梢枯病的新病原真菌，为明确其生物特性，采用生长速率法观察不同温度、光照条件、pH值、培养基、碳源和氮源对其菌丝体生长的影响。结果表明：福建平脐蠕孢菌菌丝体在PDA培养基25℃条件下生长速度为1.61 cm/d，最适生长温度范围为25～30℃，最适宜光照条件是12 h光暗交替，pH值5～13范围内均可正常生长，最适pH值范围为6～9。在不同的培养基上，菌丝体生长差异显著，其中在黄豆、燕麦蔗糖、玉米粉3种培养基上均生长较好，菌落生长速度快；最适碳源为蔗糖和果糖，最适氮源为牛肉浸膏。该结果可为由该病原菌引起的杉木梢枯病的流行规律调查及防治措施制定提供理论基础。
Keyword ：
杉木 杉木 梢枯病 梢枯病 生物学特性 生物学特性 福建平脐蠕孢菌 福建平脐蠕孢菌
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| GB/T 7714 | 薛丽丽 , 陈春莉 , 王晓亭 et al. 杉木梢枯病病原菌Bipolaris fujianensis菌丝体的生物学特性 [J]. | 中国森林病虫 , 2024 , 43 (06) : 12-17 . |
| MLA | 薛丽丽 et al. "杉木梢枯病病原菌Bipolaris fujianensis菌丝体的生物学特性" . | 中国森林病虫 43 . 06 (2024) : 12-17 . |
| APA | 薛丽丽 , 陈春莉 , 王晓亭 , 滕剑 , 黎江南 , 张清华 . 杉木梢枯病病原菌Bipolaris fujianensis菌丝体的生物学特性 . | 中国森林病虫 , 2024 , 43 (06) , 12-17 . |
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本发明属于微生物领域，具体涉及一株贝莱斯芽孢杆菌及其应用，所述菌为芽孢杆菌（Bacillusvelezensis）N3，已于2024年5月29日于中国典型培养物保藏中心保藏，保藏号为CCTCC M 20241090。N3菌株对福建柏枝枯病有显著的防治效果，是一株具有生防潜力的芽孢杆菌菌株。对该菌株生防潜力的探究，使其更好地运用于福建柏枝枯病的生物防治实践中，为建立友好型生态环境提供科学依据。
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| GB/T 7714 | 张清华 , 卢颖 , 许晓晨 et al. 一株贝莱斯芽孢杆菌及其应用 : CN202410954813.8[P]. | 2024-07-17 . |
| MLA | 张清华 et al. "一株贝莱斯芽孢杆菌及其应用" : CN202410954813.8. | 2024-07-17 . |
| APA | 张清华 , 卢颖 , 许晓晨 , 林斌 , 罗锐丰 . 一株贝莱斯芽孢杆菌及其应用 : CN202410954813.8. | 2024-07-17 . |
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本发明属于微生物领域，具体涉及一种链霉菌发酵液及其制备方法和应用，所述菌为南平链霉菌（Streptomyces nanpingensis sp.nov.）P86，已于2021年10月7日于中国典型培养物保藏中心保藏，保藏号为CCTCCM20211202。链霉菌P86是一株具有生防潜力的链霉菌菌株，对毛色二孢属真菌抑菌防病效果显著。对该菌株生防潜力的探究，使其更好地运用于生物防治实践中，为建立友好型生态环境提供科学依据。
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| GB/T 7714 | 张清华 , 杨佩文 , 陈少如 et al. 一种链霉菌发酵液及其制备方法和应用 : CN202410954883.3[P]. | 2024-07-17 . |
| MLA | 张清华 et al. "一种链霉菌发酵液及其制备方法和应用" : CN202410954883.3. | 2024-07-17 . |
| APA | 张清华 , 杨佩文 , 陈少如 , 廖依然 , 慕乐乐 , 兰彧飏 . 一种链霉菌发酵液及其制备方法和应用 : CN202410954883.3. | 2024-07-17 . |
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Chinese fir is an extremely important economic tree species in southern China. In recent years, 74.5% of Chinese fir saplings suffered from shoot blight in Shunchang County, Nanping City, Fujian Province, China. Seventeen isolates were collected from rotten shoots, and their pathogenicity was confirmed following Koch's postulates. The five pathogenic isolates were identified as belonging to the genus Bipolaris based on morphological characteristics, including septate and geniculate conidiophores, smooth to slightly verruculose conidiogenous nodes, dematiaceous phragmospore conidia, oblong or fusiform conidia, and slightly protruding or truncate hilum on conidia, but the number of pseudosepta (3 to 11, mostly 5 to 8) and the size of conidia ([22.81 to 116.13] x [9.16 to 26.58] mu m) are different from those of the known species of Bipolaris. A phylogenetic analysis based on ITS, GAPDH, and Tef1-alpha sequences determined that the five strains belong to a new species of Bipolaris, and the name Bipolaris fujianensis sp. nov. is proposed. The fungicide sensitivity of the pathogen strain Cfsb3 was further evaluated using eight fungicides. Flusilazole, difenoconazole, tebuconazole, and propiconazole exhibited high toxicity to Cfsb3, and the effective concentration inhibiting 50% (EC50) of mycelial growth was 0.08, 0.20, 0.34, and 0.36 mu g/ml, respectively, for these four fungicides. Flusilazole, difenoconazole, and iprodione inhibited B. fujianensis by 100% on detached Chinese fir shoots at their recommended concentrations, but azoxystrobin and thiram were ineffective. In conclusion, this study reported an emerging pathogen of Chinese fir sapling shoot blight and proposed triazole and dicarboximide fungicides for disease control.
Keyword ：
Bipolaris fujianensis sp. nov. Bipolaris fujianensis sp. nov. chemical control chemical control Chinese fir Chinese fir shoot blight shoot blight
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| GB/T 7714 | Zhang, Qinghua , Meng, Yuhan , Zhao, Wenbao et al. Bipolaris fujianensis sp. nov., an Emerging Pathogen of Sapling Shoot Blight on Chinese Fir, and Its Sensitivity to Fungicides [J]. | PLANT DISEASE , 2024 , 108 (4) : 1025-1032 . |
| MLA | Zhang, Qinghua et al. "Bipolaris fujianensis sp. nov., an Emerging Pathogen of Sapling Shoot Blight on Chinese Fir, and Its Sensitivity to Fungicides" . | PLANT DISEASE 108 . 4 (2024) : 1025-1032 . |
| APA | Zhang, Qinghua , Meng, Yuhan , Zhao, Wenbao , Wang, Qianqian , Wang, Xiaoting , Xue, Lili et al. Bipolaris fujianensis sp. nov., an Emerging Pathogen of Sapling Shoot Blight on Chinese Fir, and Its Sensitivity to Fungicides . | PLANT DISEASE , 2024 , 108 (4) , 1025-1032 . |
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Epicoccum sorghinum Epicoccum sorghinum leaf tip blight leaf tip blight Phoebe bournei Phoebe bournei
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| GB/T 7714 | Wang, Qianqian , Xu, Xiaochen , Zhao, Wenbao et al. First Report of Leaf Tip Blight Caused by Epicoccum sorghinum on Phoebe bournei in Fujian, China [J]. | PLANT DISEASE , 2023 , 107 (8) . |
| MLA | Wang, Qianqian et al. "First Report of Leaf Tip Blight Caused by Epicoccum sorghinum on Phoebe bournei in Fujian, China" . | PLANT DISEASE 107 . 8 (2023) . |
| APA | Wang, Qianqian , Xu, Xiaochen , Zhao, Wenbao , He, Yuying , Gao, Hongli , Xie, Shiyuan et al. First Report of Leaf Tip Blight Caused by Epicoccum sorghinum on Phoebe bournei in Fujian, China . | PLANT DISEASE , 2023 , 107 (8) . |
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