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学者姓名：帅鹏
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Abstract ：
为探究杉木ClC3HDZ1转录因子响应缺磷胁迫的作用机制。通过构建杉木ClC3HDZ1转录因子过表达拟南芥植株，研究其对缺磷胁迫的响应，提取杉木叶片中的总RNA并将其反转为cDNA,利用三步法聚合酶链式反应（PCR）扩增出ClC3HDZ1转录因子的编码序列片段，连接至带有35S启动子驱动的PCAMBIA1300载体，转化到农杆菌，通过花序侵染法导入野生型拟南芥植株。经含有潮霉素的MS固体培养基筛选和PCR鉴定获得过表达植株，培养至T
Keyword ：
ClC3HDZ1基因 ClC3HDZ1基因 拟南芥 拟南芥 杉木 杉木 缺磷胁迫 缺磷胁迫 过表达 过表达
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| GB/T 7714 | 黄朝章 , 赵紫宇 , 曾一帆 et al. 拟南芥过表达杉木ClC3HDZ1基因及其对缺磷胁迫的响应 [J]. | 森林与环境学报 , 2025 , 45 (05) : 501-508 . |
| MLA | 黄朝章 et al. "拟南芥过表达杉木ClC3HDZ1基因及其对缺磷胁迫的响应" . | 森林与环境学报 45 . 05 (2025) : 501-508 . |
| APA | 黄朝章 , 赵紫宇 , 曾一帆 , 叶小鹏 , 马祥庆 , 帅鹏 . 拟南芥过表达杉木ClC3HDZ1基因及其对缺磷胁迫的响应 . | 森林与环境学报 , 2025 , 45 (05) , 501-508 . |
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Chinese fir (Cunninghamia lanceolata (Lamb.) Hook) is one of the main afforestation tree species in southern China. Continuous planting for multiple generations has led to a decrease in the content of available phosphorus in the soil. To adapt to low phosphorus stress, plants develop a series of physiological, biochemical, and developmental responses through self-regulation. Recent studies have shown that miRNAs play a regulatory role in plants' responses to low phosphorus stress. However, the regulatory mechanism of miRNAs in Chinese fir in response to low phosphorus stress is still unclear. Here, we performed small RNA sequencing on the Chinese fir roots treated with normal phosphorus and low phosphorus and identified a total of 321 miRNAs, including 139 known miRNAs and 182 new miRNAs, with 43 differentially expressed miRNAs (DEMs). Integrative analysis combined with degradome sequencing data revealed that 193 miRNAs (98 known and 95 new) targeted 469 genes, among which 23 DEMs targeted 44 genes. Gene enrichment analysis indicated that under low phosphorus stress, transcription and transcriptional regulation, as well as signal transduction, were significantly activated in Chinese fir. Modules in the miRNA-target pathways, such as miR166/HD-ZIP III, miR169/NFYA7, miR529/SPL, and miR399/UBC23, may be the key regulatory factors in the response to low phosphorus stress in Chinese fir. In addition, we found that PC-3p-1033_8666 was significantly downregulated and that PC-5p-3786_2830 was significantly upregulated, which presumably respond to low phosphorus stress by indirectly affecting phosphorus-related hormone signaling or PSR genes. The identified miRNA-target network and significantly activated pathways in this study provide insights into the post-transcriptional regulatory mechanisms of Chinese fir adapting to low phosphorus environments, which can offer theoretical references for the stress resistance and superior variety breeding of Chinese fir.
Keyword ：
Cunninghamia lanceolata Cunninghamia lanceolata degradome degradome low phosphorus stress low phosphorus stress miRNA miRNA
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| GB/T 7714 | Li, Meng , Ye, Xiaopeng , Zhao, Ziyu et al. Identification of miRNAs and Their Targets in Cunninghamia lanceolata Under Low Phosphorus Stress Based on Small RNA and Degradome Sequencing [J]. | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES , 2025 , 26 (8) . |
| MLA | Li, Meng et al. "Identification of miRNAs and Their Targets in Cunninghamia lanceolata Under Low Phosphorus Stress Based on Small RNA and Degradome Sequencing" . | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 26 . 8 (2025) . |
| APA | Li, Meng , Ye, Xiaopeng , Zhao, Ziyu , Zeng, Yifan , Huang, Chaozhang , Ma, Xiangqing et al. Identification of miRNAs and Their Targets in Cunninghamia lanceolata Under Low Phosphorus Stress Based on Small RNA and Degradome Sequencing . | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES , 2025 , 26 (8) . |
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Secondary growth is a key characteristic evolved from seed plants and generates secondary xylem-the most abundant tissue on Earth. Recent studies have uncovered xylem developmental lineages in eudicots and magnoliids of angiosperms. However, xylem development in gymnosperms, the other representative clade of seed plants, remained elusive. We performed single-cell transcriptomics for xylem cells of conifers (Cunninghamia lanceolata), the major clade in gymnosperms. Using Seurat and scVI-based cross-species integration, we reconstructed the xylem differentiation trajectories and revealed that the radial system is conserved across seed plants, while the axial system in C. lanceolata exhibits a composite lineage architecture resembling both eudicots and magnoliids. To validate these trajectories, we established a multi-modal spatial framework incorporating spatial transcriptomics, spatial proteomics, and spatial metabolomics. These three spatial layers provided orthogonal evidence confirming cell-type identities and trajectory inference. Additionally, we identified a xylem cell population unique to gymnosperms, suggesting a lineage-specific specialization. Together, our findings uncover a more complex ancestral xylem architecture in gymnosperms and propose a progressive simplification of axial developmental programs from gymnosperms to angiosperms, highlighting a trajectory of reductive evolution in seed plant vascular development. Single-cell and spatial omics in conifers uncover conserved radial lineages but divergent axial trajectories with angiosperm-like and gymnosperm-specific features, revealing reductive evolution.
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| GB/T 7714 | Shuai, Peng , Hsieh, Jo-Wei Allison , Kao, Chung-Ting et al. Single-cell and spatial omics reveal progressive loss of xylem developmental complexity across seed plants [J]. | PLANT CELL , 2025 , 37 (11) . |
| MLA | Shuai, Peng et al. "Single-cell and spatial omics reveal progressive loss of xylem developmental complexity across seed plants" . | PLANT CELL 37 . 11 (2025) . |
| APA | Shuai, Peng , Hsieh, Jo-Wei Allison , Kao, Chung-Ting , Hu, Chen-Wei , Wang, Ray , Kuo, Shang-Che et al. Single-cell and spatial omics reveal progressive loss of xylem developmental complexity across seed plants . | PLANT CELL , 2025 , 37 (11) . |
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为探究修枝处理下幼林杉木节子伤口愈合情况，以福建省邵武卫闽国有林场6年生杉木人工林为研究对象，设计不同修枝强度、保留密度、节子方向、伤口直径、喷漆保护等试验，统计分析修枝20个月后幼林杉木节子的伤口愈合率变化。结果表明：不同修枝强度对幼林杉木节子的横向伤口愈合率有显著影响（P<0.05）,横向伤口愈合率大小依次为10 cm修枝强度>12 cm修枝强度>8 cm修枝强度。保留密度为1 800株·hm
Keyword ：
伤口愈合 伤口愈合 保留密度 保留密度 修枝 修枝 修枝强度 修枝强度 杉木 杉木 节子 节子
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| GB/T 7714 | 魏理晖 , 叶小鹏 , 陈志云 et al. 密度及修枝对幼林杉木节子伤口愈合的影响 [J]. | 森林与环境学报 , 2024 , 44 (06) : 639-646 . |
| MLA | 魏理晖 et al. "密度及修枝对幼林杉木节子伤口愈合的影响" . | 森林与环境学报 44 . 06 (2024) : 639-646 . |
| APA | 魏理晖 , 叶小鹏 , 陈志云 , 何宗明 , 马祥庆 , 帅鹏 . 密度及修枝对幼林杉木节子伤口愈合的影响 . | 森林与环境学报 , 2024 , 44 (06) , 639-646 . |
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| GB/T 7714 | Yuan, Tao-hua , Wang, Zi , Shen, Cai-quan et al. A taxonomic correction of Impatiens (Balsaminaceae) in Xizang, China-The record of Impatiens bracteata is a misidentification of I. darjeekhandui [J]. | PHYTOTAXA , 2024 , 672 (1) : 121-125 . |
| MLA | Yuan, Tao-hua et al. "A taxonomic correction of Impatiens (Balsaminaceae) in Xizang, China-The record of Impatiens bracteata is a misidentification of I. darjeekhandui" . | PHYTOTAXA 672 . 1 (2024) : 121-125 . |
| APA | Yuan, Tao-hua , Wang, Zi , Shen, Cai-quan , Hu, Guang-wan , Peng, Shuai . A taxonomic correction of Impatiens (Balsaminaceae) in Xizang, China-The record of Impatiens bracteata is a misidentification of I. darjeekhandui . | PHYTOTAXA , 2024 , 672 (1) , 121-125 . |
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Phosphate (Pi) deficiency is one of the most limiting factors for Chinese fir growth and production. Moreover, continuous cultivation of Chinese fir for multiple generations led to the reduction of soil nutrients, which hindered the yield of Chinese fir in southern China. Although NAC (NAM, ATAF, and CUC) transcription factors (TFs) play critical roles in plant development and abiotic stress resistance, it is still unclear how they regulate the response of Chinese fir to phosphate (Pi) starvation. Based on Pi-deficient transcriptome data of Chinses fir root, we identified a NAC transcription factor with increased expression under Pi deficiency, which was obtained by PCR and named ClNAC100. RT-qPCR confirmed that the expression of ClNAC100 in the root of Chinese fir was induced by phosphate deficiency and showed a dynamic change with time. It was positively regulated by ABA and negatively regulated by JA, and ClNAC100 was highly expressed in the roots and leaves of Chinese fir. Transcriptional activation assay confirmed that ClNAC100 was a transcriptional activator. The promoter of ClNAC100 was obtained by genome walking, which was predicted to contain a large number of stress, hormone, and growth-related cis-elements. Tobacco infection was used to verify the activity of the promoter, and the core promoter was located between -1519 bp and -589 bp. We identified 18 proteins bound to the ClNAC100 promoter and 5 ClNAC100 interacting proteins by yeast one-hybrid and yeast two-hybrid, respectively. We speculated that AHL and TIFY family transcription factors, calmodulin, and E3 ubiquitin ligase in these proteins might be important phosphorus-related proteins. These results provide a basis for the further study of the regulatory mechanism and pathways of ClNAC100 under Pi starvation.
Keyword ：
Cunninghamia lanceolata Cunninghamia lanceolata hormone hormone NAC NAC phosphate starvation phosphate starvation stress stress
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| GB/T 7714 | Zhao, Yuxuan , Huang, Shuotian , Wei, Lihui et al. ClNAC100 Is a NAC Transcription Factor of Chinese Fir in Response to Phosphate Starvation [J]. | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES , 2023 , 24 (13) . |
| MLA | Zhao, Yuxuan et al. "ClNAC100 Is a NAC Transcription Factor of Chinese Fir in Response to Phosphate Starvation" . | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 24 . 13 (2023) . |
| APA | Zhao, Yuxuan , Huang, Shuotian , Wei, Lihui , Li, Meng , Cai, Tingting , Ma, Xiangqing et al. ClNAC100 Is a NAC Transcription Factor of Chinese Fir in Response to Phosphate Starvation . | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES , 2023 , 24 (13) . |
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Background: Xylem, the most abundant tissue on Earth, is responsible for lateral growth in plants. Typical xylem has a radial system composed of ray parenchyma cells and an axial system of fusiform cells. In most angiosperms, fusiform cells comprise vessel elements for water transportation and libriform fibers for mechanical support, while both functions are performed by tracheids in other vascular plants such as gymnosperms. Little is known about the developmental programs and evolutionary relationships of these xylem cell types. Results: Through both single-cell and laser capture microdissection transcriptomic profiling, we determine the developmental lineages of ray and fusiform cells in stem-differentiating xylem across four divergent woody angiosperms. Based on cross-species analyses of single-cell clusters and overlapping trajectories, we reveal highly conserved ray, yet variable fusiform, lineages across angiosperms. Core eudicots Populus trichocarpa and Eucalyptus grandis share nearly identical fusiform lineages, whereas the more basal angiosperm Liriodendron chinense has a fusiform lineage distinct from that in core eudicots. The tracheids in the basal eudicot Trochodendron aralioides, an evolutionarily reversed trait, exhibit strong transcriptomic similarity to vessel elements rather than libriform fibers. Conclusions: This evo-devo framework provides a comprehensive understanding of the formation of xylem cell lineages across multiple plant species spanning over a hundred million years of evolutionary history.
Keyword ：
Cross-species comparison Cross-species comparison Libriform fiber Libriform fiber Ray parenchyma cell Ray parenchyma cell Single-cell RNA sequencing Single-cell RNA sequencing Tissue evolution Tissue evolution Tracheid Tracheid Trait reversal Trait reversal Vessel element Vessel element Wood formation Wood formation Xylem Xylem
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| GB/T 7714 | Tung, Chia-Chun , Kuo, Shang-Che , Yang, Chia-Ling et al. Single-cell transcriptomics unveils xylem cell development and evolution [J]. | GENOME BIOLOGY , 2023 , 24 (1) . |
| MLA | Tung, Chia-Chun et al. "Single-cell transcriptomics unveils xylem cell development and evolution" . | GENOME BIOLOGY 24 . 1 (2023) . |
| APA | Tung, Chia-Chun , Kuo, Shang-Che , Yang, Chia-Ling , Yu, Jhong-He , Huang, Chia-En , Liou, Pin-Chien et al. Single-cell transcriptomics unveils xylem cell development and evolution . | GENOME BIOLOGY , 2023 , 24 (1) . |
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Chinese fir (Cunninghamia lanceolata (Lamb.) Hook) is a widely grown gymnosperm in China. Phosphorus (P) is an indispensable nutrient for the growth of Chinese fir. Inorganic phosphate (Pi) deficiency exists in soils of many Chinese fir planting area regions, and the trees themselves have limited efficiency in utilizing P from the soil. Ethylene is important in regulation responses to nutrient deficiencies. However, little is known about how ethylene signals participate in Pi stress in Chinese fir. A total of six different treatments were performed to reveal the transcript levels of Chinese fir under Pi, ethephon (an ethylene-releasing compound), and CoCl2 (cobalt chloride, an ethylene biosynthesis inhibitor) treatments. We assembled a full-length reference transcriptome containing 22,243 unigenes as a reference for UMI RNA-seq (Digital RNA-seq). There were 586 Differentially Expressed Genes (DEGs) in the Pi starvation (NP) group, while DEGs from additional ethephon or CoCl2 in NP were 708 and 292, respectively. Among the DEGs in each treatment, there were 83 TFs in these treatment groups. MYB (v-myb avian myeloblastosis viral oncogene homolog) family was the most abundant transcription factors (TFs). Three ERF (Ethylene response factor) family genes were identified when only ethylene content was imposed as a variable. Enrichment analysis indicated that the ascorbate and aldarate metabolism pathway plays a key role in resistance to Pi deficiency. This study provides insights for further elucidating the regulatory mechanism of Pi deficiency in Chinese fir.
Keyword ：
Cunninghamia lanceolata Cunninghamia lanceolata ethephon ethephon phosphorus treatment phosphorus treatment transcription factor transcription factor UMI RNA-seq UMI RNA-seq
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| GB/T 7714 | Huang, Shuotian , Zhang, Lixia , Cai, Tingting et al. Transcriptome Level Analysis of Genes of Exogenous Ethylene Applied under Phosphorus Stress in Chinese Fir [J]. | PLANTS-BASEL , 2022 , 11 (15) . |
| MLA | Huang, Shuotian et al. "Transcriptome Level Analysis of Genes of Exogenous Ethylene Applied under Phosphorus Stress in Chinese Fir" . | PLANTS-BASEL 11 . 15 (2022) . |
| APA | Huang, Shuotian , Zhang, Lixia , Cai, Tingting , Zhao, Yuxuan , Liu, Jiao , Wu, Pengfei et al. Transcriptome Level Analysis of Genes of Exogenous Ethylene Applied under Phosphorus Stress in Chinese Fir . | PLANTS-BASEL , 2022 , 11 (15) . |
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【目的】为了完善杉木NAC基因家族的生信机制以及探索基因潜在功能,促进杉木基因改良育种与提高生产效率。【方法】本研究从搜集到的杉木转录组测序数据库中筛选鉴定得到杉木NAC基因,并对其进行生物信息学分析,具体包括蛋白的理化性质、结构域预测、基因进化树分析和cln-miR164靶基因的预测分析等。【结果】杉木NAC转录因子家族含有45个成员,将其命名为Cl-NAC1～Cl-NAC45,预测所得CDS序列长度在156～3 033 bp之间,由51～1 010个氨基酸残基构成,结构域分析得出Cl-NACs蛋白除了长度不足的序列,所有的NAC蛋白都具有A、B、C、D、E 5个亚结构域,并且位置一致。保守域预测可得不同亚家族7个保守结构域的位置和长度有所不同。大部分Cl-NACs基因与挪威云杉NAC基因序列相似度明显大于拟南芥。通过进化树分析,不同Cl-NACs蛋白分布在10个亚家族中ATAF亚家族中的Cl-NAC45可能与逆境胁迫相关,NAM亚家族中的Cl-NAC1、Cl-NAC33、Cl-NAC34和Cl-NAC35基因都有可能具有调节杉木生长发育的作用;此外通过psRNATarget预测Cl-NAC33和Cl-NAC35为cln-miR164的靶基因,可能受其调控,参与非生物胁迫响应途径。【结论】杉木NAC基因鉴定获得45个,共可分为10个亚家族,不同的亚家族具有不同的理化性质、蛋白质结构、进化模式。对不同亚家族的基因功能进行初步推测,上述7个基因可能为杉木生长发育中的关键基因,需进一步研究验证其功能,为后续研究Cl-NACs基因对杉木生长发育、抗逆性功能奠定了基础。
Keyword ：
NAC基因家族 NAC基因家族 杉木 杉木 生物学信息分析 生物学信息分析 鉴定 鉴定
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| GB/T 7714 | 高文杰 , 刘娇 , 马祥庆 et al. 杉木NAC基因家族基因的鉴定及生物信息学分析 [J]. | 中南林业科技大学学报 , 2022 , 42 (02) : 108-118 . |
| MLA | 高文杰 et al. "杉木NAC基因家族基因的鉴定及生物信息学分析" . | 中南林业科技大学学报 42 . 02 (2022) : 108-118 . |
| APA | 高文杰 , 刘娇 , 马祥庆 , 帅鹏 . 杉木NAC基因家族基因的鉴定及生物信息学分析 . | 中南林业科技大学学报 , 2022 , 42 (02) , 108-118 . |
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【目的】研究5种杉木幼林不同龄期生长特性，为杉木人工林高效培育技术体系的构建提供理论依据。【方法】采用随机区组试验设计，共设置5种处理（洋020、洋061、广西二代、福建埔上三代营造纯林、洋020和洋061株间混交造林），比较其生长过程的平均胸径、树高、东西冠幅、南北冠幅、单株立木材积、径阶分布等的变化情况。【结果】各杉木良种的胸径、树高、冠幅和单株材积生长的良种效应差异多数达到显著性水平；洋061与洋061（混交）良种的平均胸径、树高、冠幅生长量变异都很小，洋061良种造林时林分分布更整齐；福建三代标准地径阶分布图形明显呈正态分布，具有良好的空间结构，洋061（混交）与洋061良种在8～12 cm径阶林木株树占总株数的百分比最多，分别为60.45%、41.81%。【结论】洋061良种林木各项生长指标表现最好，拥有大量的大径阶林木，在造林初期杉木洋061良种效应相较于其他良种更有优势，具有高效培育杉木人工林的优质潜力。
Keyword ：
幼龄林 幼龄林 径阶 径阶 杉木 杉木 材积 材积 遗传控制 遗传控制
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| GB/T 7714 | 张丽霞 , 曹光球 , 林开敏 et al. 5种杉木幼林不同龄期生长特性比较 [J]. | 福建农业学报 , 2022 , 37 (07) : 904-911 . |
| MLA | 张丽霞 et al. "5种杉木幼林不同龄期生长特性比较" . | 福建农业学报 37 . 07 (2022) : 904-911 . |
| APA | 张丽霞 , 曹光球 , 林开敏 , 马祥庆 , 帅鹏 . 5种杉木幼林不同龄期生长特性比较 . | 福建农业学报 , 2022 , 37 (07) , 904-911 . |
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