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学者姓名：罗永海
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Abstract ：
开花是被子植物的重要生物学行为,关系到植物种群的维持与繁衍.CONSTANS(CO)和FLOWERING LOCUS T(FT)是植物开花分子调控网络中的2个关键基因,形成CO/FT途径协同作用,受光周期途径调控,在植物成花转变过程中扮演枢纽的角色.CO/FT途径有古老的系统发育源头,在物种进化过程中处于调控个体生长与繁育的核心位置,在同一物种内或不同物种间存在高度的功能多样性,参与了众多生物学途径或植物器官发育调控.本文总结了CO/FT途径及其所在基因家族成员的生物学功能及其分化,结合近年来的新进展,对CO/FT途径的研究进行了展望.
Keyword ：
CO CO FT FT 光周期 光周期 功能分化 功能分化 基因家族 基因家族
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| GB/T 7714 | 罗碧珍 , 罗永海 . 开花植物CO/FT分子途径的生物学功能和分子进化 [J]. | 福建农林大学学报(自然科学版) , 2021 , 50 (02) : 155-163 . |
| MLA | 罗碧珍 等. "开花植物CO/FT分子途径的生物学功能和分子进化" . | 福建农林大学学报(自然科学版) 50 . 02 (2021) : 155-163 . |
| APA | 罗碧珍 , 罗永海 . 开花植物CO/FT分子途径的生物学功能和分子进化 . | 福建农林大学学报(自然科学版) , 2021 , 50 (02) , 155-163 . |
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Abstract ：
Background. Sweet potato (Ipomoea batatas (L.) Lam.) is one of the most important crops in many developing countries and provides a candidate source of bioenergy. However, neither a complete reference genome nor large-scale full-length cDNA sequences for this outcrossing hexaploid crop are available, which in turn impedes progress in research studies in I. batatas functional genomics and molecular breeding. Methods. In this study, we sequenced full-length transcriptomes in I. batatas and its diploid ancestor I. trifida by single-molecule real-time sequencing and Illumina second-generation sequencing technologies. With the generated datasets, we conducted comprehensive intraspecific and interspecific sequence analyses and experimental characterization. Results. A total of 53,861/51,184 high-quality long-read transcripts were obtained, which covered about 10,439/10,452 loci in the I. batatas/I. trifida genome. These datasets enabled us to predict open reading frames successfully in 96.83%/96.82% of transcripts and identify 34,963/33,637 full-length cDNA sequences, 1,401/1,457 transcription factors, 25,315/27,090 simple sequence repeats, 1,656/1,389 long non-coding RNAs, and 5,251/8,901 alternative splicing events. Approximately, 32.34%/38.54% of transcripts and 46.22%/51.18% multi-exon transcripts underwent alternative splicing in I. batatas/I. trifida. Moreover, we validated one alternative splicing event in each of 10 genes and identified tuberous-root-specific expressed isoforms from a starch-branching enzyme, an alpha-glucan phosphorylase, a neutral invertase, and several ABC transporters. Overall, the collection and analysis of large-scale long-read transcripts generated in this study will serve as a valuable resource for the I. batatas research community, which may accelerate the progress in its structural, functional, and comparative genomics studies.
Keyword ：
Alternative splicing Alternative splicing Comparative transcriptome analysis Comparative transcriptome analysis Single-molecular real-time sequencing Single-molecular real-time sequencing Sweet potato Sweet potato
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| GB/T 7714 | Ding, Na , Cui, Huihui , Miao, Ying et al. Single-molecule real-time sequencing identifies massive full-length cDNAs and alternative-splicing events that facilitate comparative and functional genomics study in the hexaploid crop sweet potato [J]. | PEERJ , 2019 , 7 . |
| MLA | Ding, Na et al. "Single-molecule real-time sequencing identifies massive full-length cDNAs and alternative-splicing events that facilitate comparative and functional genomics study in the hexaploid crop sweet potato" . | PEERJ 7 (2019) . |
| APA | Ding, Na , Cui, Huihui , Miao, Ying , Tan, Jun , Cao, Qinghe , Luo, Yonghai . Single-molecule real-time sequencing identifies massive full-length cDNAs and alternative-splicing events that facilitate comparative and functional genomics study in the hexaploid crop sweet potato . | PEERJ , 2019 , 7 . |
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To study the diversity and cultivar-specific of phytochemicals in sweet potato, Liquid Chromatography-Electrospray Ionization-Mass Spectrometry was used to analyze the metabolic profiles of five sweet potato cultivars exhibiting different flesh colors: purple, yellow/orange, and white. A total of 213 metabolites, including 29 flavonoids and 27 phenolic acids, were characterized. The flavonoid profiles of the five different cultivars were distinguished using PCA, the results suggested the flesh color accounted for the observed metabolic differences. In addition to anthocyanins, quinic acids and ferulic acids were the prominent phenolic acids, O-hexoside of quercetin, chrysoeriol were the prominent flavonoids in sweet potato tubers, and they were all higher in the OFSP and PFSP than WFSP. The main differential metabolic pathways between the OFSP, PFSP and the WFSP included those relating to phenylpropanoid and flavonoid biosynthesis. This study provides new in-sights into the differences in metabolite profiles among sweet potatoes with different flesh colors.
Keyword ：
Flavonoids Flavonoids Metabolomics Metabolomics Phenolic acids Phenolic acids Sweet potato Sweet potato
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| GB/T 7714 | Wang, Aimin , Li, Rensai , Ren, Lei et al. A comparative metabolomics study of flavonoids in sweet potato with different flesh colors (Ipomoea batatas (L.) Lam) [J]. | FOOD CHEMISTRY , 2018 , 260 : 124-134 . |
| MLA | Wang, Aimin et al. "A comparative metabolomics study of flavonoids in sweet potato with different flesh colors (Ipomoea batatas (L.) Lam)" . | FOOD CHEMISTRY 260 (2018) : 124-134 . |
| APA | Wang, Aimin , Li, Rensai , Ren, Lei , Gao, Xiali , Zhang, Yungang , Ma, Zhimin et al. A comparative metabolomics study of flavonoids in sweet potato with different flesh colors (Ipomoea batatas (L.) Lam) . | FOOD CHEMISTRY , 2018 , 260 , 124-134 . |
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Background: Sweet potato, a hexaploid species lacking a reference genome, is one of the most important crops in many developing countries, where abiotic stresses are a primary cause of reduction of crop yield. Glutathione S-transferases (GSTs) are multifunctional enzymes that play important roles in oxidative stress tolerance and cellular detoxification. Results: A total of 42 putative full-length GST genes were identified from two local transcriptome databases and validated by molecular cloning and Sanger sequencing. Sequence and intraspecific phylogenetic analyses revealed extensive differentiation in their coding sequences and divided them into eight subfamilies. Interspecific phylogenetic and comparative analyses indicated that most examined GST paralogs might originate and diverge before the speciation of sweet potato. Results from large-scale RNA-seq and quantitative real-time PCR experiments exhibited extensive variation in gene-expression profiles across different tissues and varieties, which implied strong evolutionary divergence in their gene-expression regulation. Moreover, we performed five manipulated stress experiments and uncovered highly divergent stress-response patterns of sweet potato GST genes in aboveground and underground tissues. Conclusions: Our study identified a large number of sweet potato GST genes, systematically investigated their evolutionary diversification, and provides new insights into the GST-mediated stress-response mechanisms in this worldwide crop.
Keyword ：
Abiotic stresses Abiotic stresses Evolutionary diversification Evolutionary diversification Glutathione S-transferase Glutathione S-transferase Regulatory mechanism Regulatory mechanism Sweet potato Sweet potato
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| GB/T 7714 | Ding, Na , Wang, Aimin , Zhang, Xiaojun et al. Identification and analysis of glutathione S-transferase gene family in sweet potato reveal divergent GST-mediated networks in aboveground and underground tissues in response to abiotic stresses [J]. | BMC PLANT BIOLOGY , 2017 , 17 . |
| MLA | Ding, Na et al. "Identification and analysis of glutathione S-transferase gene family in sweet potato reveal divergent GST-mediated networks in aboveground and underground tissues in response to abiotic stresses" . | BMC PLANT BIOLOGY 17 (2017) . |
| APA | Ding, Na , Wang, Aimin , Zhang, Xiaojun , Wu, Yunxiang , Wang, Ruyuan , Cui, Huihui et al. Identification and analysis of glutathione S-transferase gene family in sweet potato reveal divergent GST-mediated networks in aboveground and underground tissues in response to abiotic stresses . | BMC PLANT BIOLOGY , 2017 , 17 . |
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