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学者姓名：郑祥梓
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本发明涉及植物种子种植技术领域，具体涉及一种水稻种子解除休眠的方法，具体包括以下步骤：1)种子选择：选取4℃种窖贮存的处于休眠状态的水稻种子；2)将步骤1)选取的水稻种子在促解除休眠药剂中浸泡24小时后，用清水洗净；3)在28℃恒温下进行标准发芽实验，观察记载发芽情况。本发明优点在于：步骤设计合理，便于操作，针对水稻种子解除休眠的效果显著。可以方便地应用于实际生产中，提高种子的利用率和种植效益。
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| GB/T 7714 | 汪小力 , 王玮琦 , 郑祥梓 et al. 一种水稻种子解除休眠的方法 : CN202411843420.6[P]. | 2024-12-14 . |
| MLA | 汪小力 et al. "一种水稻种子解除休眠的方法" : CN202411843420.6. | 2024-12-14 . |
| APA | 汪小力 , 王玮琦 , 郑祥梓 , 缪颖 . 一种水稻种子解除休眠的方法 : CN202411843420.6. | 2024-12-14 . |
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本发明涉及水仙花原生质体技术领域，具体涉及一种水仙花原生质体分离与转化体系建立方法，具体包括以下步骤：配置10mL buffer；避光抽真空30min；取出，用枪头吸除buffer；避光抽真空30min；室温酶解消化；释放原生质体；洗涤原生质体并终止酶解，离心，弃上清，留沉淀；分层；离心，分为3层，原生质体转移；用另一只离心管平衡并离心，弃上清，留沉淀，加W5至5mL轻轻晃动悬浮原生质体；镜检；使原生质体重悬复苏，离心；弃上清，留沉淀，原生质体的转化体系建立；本发明优点在于：高效、简便地针对水仙花原生质体分离与转化体系建立，为水仙花的遗传改良和生物技术研究提供有力的工具。
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| GB/T 7714 | 汪小力 , 吴卓豫 , 杨静文 et al. 一种水仙花原生质体分离与转化体系建立方法 : CN202411843426.3[P]. | 2024-12-14 . |
| MLA | 汪小力 et al. "一种水仙花原生质体分离与转化体系建立方法" : CN202411843426.3. | 2024-12-14 . |
| APA | 汪小力 , 吴卓豫 , 杨静文 , 郑祥梓 , 缪颖 . 一种水仙花原生质体分离与转化体系建立方法 : CN202411843426.3. | 2024-12-14 . |
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Leaf senescence triggers major metabolic changes that recycle resources and ensure plant survival and is sensitive to environmental conditions. In this study, we characterized OsS40-14 using CRISPR/Ca9 mutants and overexpression lines to address its function in rice (Oryza sativa) flag leaf senescence. The oss40-14 mutants displayed a stay-green leaf phenotype under dark treatment, with preserved chlorophylls and photosynthetic capacity and reduced reactive oxygen species (ROS) levels. In contrast, OsS40-14 overexpression lines exhibited accelerated leaf senescence. Transcriptome analysis of dark-treated flag leaf samples revealed that the 1,585 differentially expressed genes in oss40-14 vs WT were enriched in macromolecular metabolism, photosynthesis, and stress responses. In addition, tsCUT&Tag-seq analysis using GFP-tagged OsS40-14 revealed 2,311 genomic loci bound by OsS40-14, with approximately 40.95% of the binding signal enriched at transcription start sites. The consensus binding motif of OsS40-14 was identified as TACCCACAAGACAC, with a seed sequence of "ACCCA." Finally, integrated analysis of the transcriptome and tsCUT&Tag-seq data sets revealed that 153 OsS40-14-targeted candidates comprised 66 potentially repressed genes enriched in plastid organization and photosynthetic processes and 41 putatively activated genes associated with stress signaling and senescence. Overall, our results suggest that OsS40-14 fine-tunes the photosynthetic machinery and ROS homeostasis during developmental senescence and under dark conditions, thereby accelerating leaf senescence, reducing photosynthetic assimilation and grain filling in rice. The rice transcription factor S40-14 plays a positive role in dark-induced leaf senescence by regulating chloroplast and ROS-related gene network to adapt the changing environment for plant survival.
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| GB/T 7714 | Habiba , Fan, Chunlan , Hong, Wuqiang et al. The rice transcription factor S40-14 regulates chloroplast and reactive oxygen species-related gene networks and promotes dark-induced leaf senescence [J]. | PLANT PHYSIOLOGY , 2025 , 199 (1) . |
| MLA | Habiba et al. "The rice transcription factor S40-14 regulates chloroplast and reactive oxygen species-related gene networks and promotes dark-induced leaf senescence" . | PLANT PHYSIOLOGY 199 . 1 (2025) . |
| APA | Habiba , Fan, Chunlan , Hong, Wuqiang , Wang, Weiqi , Shi, Ximiao , Wang, Xiaowei et al. The rice transcription factor S40-14 regulates chloroplast and reactive oxygen species-related gene networks and promotes dark-induced leaf senescence . | PLANT PHYSIOLOGY , 2025 , 199 (1) . |
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Plants are continuously challenged by pathogenic threats, including fungi, bacteria, and viruses, across all stages of their growth and development. To combat these, plants have evolved a sophisticated innate immune system, with MAMP-triggered immunity (MTI) serving as an important early defense mechanism. The causative agent of late blight, Phytophthora infestans, secretes effector proteins such as SFI5 that suppress host immune responses, resulting in severe damage. This study explores the role of SFI5 in modulating MTI in host plants. Bioinformatics research revealed a conserved ATP/GTP-binding motif in the N-terminal domain of SFI5, with Lys82 being critical for effector function. Lys82 mutation lowered SFI5 ' s capacity to suppress flg22-triggered reactive oxygen species (ROS) and calcium bursts in tomato protoplasts, but it had no effect on its interaction with calmodulinbinding proteins or kinase activity. In vitro assays confirmed that SFI5 exhibits GTPase activity, unaffected by the Lys82 mutation or the presence of calmodulin. Pull-down assays combined with protein spectrum analysis revealed NbPHB1, a positive regulator of MTI, as a potential binding partner of SFI5. Functional assay demonstrated that NbPHB1 promotes MTI marker gene expression and decreases lesion size in Nicotiana benthamiana, whereas virus-induced gene silencing (VIGS) of NbPHB1 weakens MTI and increases lesion size. SFI5 suppresses NbPHB1 expression, inhibiting MTI and exacerbating lesion development during infection. In conclusion, SFI5 targets the MTI regulator NbPHB1 to inhibit immune responses, with its ATP/GTP-binding motif playing a key role in effector function. These findings provide insights into P. infestans pathogenicity and suggest potential targets for developing resistant crops.
Keyword ：
NbPHB1 NbPHB1 Phytophthora infestans Phytophthora infestans Plant MTI immunity Plant MTI immunity RxLR effector protein SFI5 RxLR effector protein SFI5
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| GB/T 7714 | Habiba, Jian , Mu, Jian , Wang, Weiqi et al. RXLR effector SFI5 of Phytophthora infestans suppress MAMP-triggered immunity via inhibition of NbPHB1 in Nicotiana benthamiana [J]. | PLANT STRESS , 2025 , 16 . |
| MLA | Habiba, Jian et al. "RXLR effector SFI5 of Phytophthora infestans suppress MAMP-triggered immunity via inhibition of NbPHB1 in Nicotiana benthamiana" . | PLANT STRESS 16 (2025) . |
| APA | Habiba, Jian , Mu, Jian , Wang, Weiqi , Dou, Zhuoning , Liao, Dongmei , Dai, Huiling et al. RXLR effector SFI5 of Phytophthora infestans suppress MAMP-triggered immunity via inhibition of NbPHB1 in Nicotiana benthamiana . | PLANT STRESS , 2025 , 16 . |
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Drought is a critical risk factor that impacts rice growth and yields. Previous studies have focused on the regulatory roles of individual transcription factors in response to drought stress. However, there is limited understanding of multi-factor stresses gene regulatory networks and their mechanisms of action. In this study, we utilised data from the JASPAR database to compile a comprehensive dataset of transcription factors and their binding sites in rice, Arabidopsis, and barley genomes. We employed the PyTorch framework for machine learning to develop a nine-layer convolutional deep neural network TFBind. Subsequently, we obtained rice RNA-seq and ATAC-seq data related to abiotic stress from the public database. Utilising integrative analysis of WGCNA and ATAC-seq, we effectively identified transcription factors associated with open chromatin regions in response to drought. Interestingly, only 81% of the transcription factors directly bound to the opened genes by testing with TFBind model. By this approach we identified 15 drought-responsive transcription factors corresponding to open chromatin regions of targets, which enriched in the terms related to protein transport, protein allocation, nitrogen compound transport. This approach provides a valuable tool for predicting TF-TAG-opened modules during biological processes.
Keyword ：
ATAC-seq ATAC-seq drought responsive TF-TAG modules drought responsive TF-TAG modules neural networks neural networks rice rice WGCNA WGCNA
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| GB/T 7714 | Liu, Jingpeng , Shi, Ximiao , Zhang, Zhitai et al. Deep Neural Network-Mining of Rice Drought-Responsive TF-TAG Modules by a Combinatorial Analysis of ATAC-Seq and RNA-Seq [J]. | PLANT CELL AND ENVIRONMENT , 2025 , 48 (7) : 5217-5235 . |
| MLA | Liu, Jingpeng et al. "Deep Neural Network-Mining of Rice Drought-Responsive TF-TAG Modules by a Combinatorial Analysis of ATAC-Seq and RNA-Seq" . | PLANT CELL AND ENVIRONMENT 48 . 7 (2025) : 5217-5235 . |
| APA | Liu, Jingpeng , Shi, Ximiao , Zhang, Zhitai , Cen, Xuexiang , Lin, Lixian , Wang, Xiaowei et al. Deep Neural Network-Mining of Rice Drought-Responsive TF-TAG Modules by a Combinatorial Analysis of ATAC-Seq and RNA-Seq . | PLANT CELL AND ENVIRONMENT , 2025 , 48 (7) , 5217-5235 . |
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Seed germination is a complex process influenced by various physical and biochemical factors, and dormancy release upon imbibition is essential for germination. However, the specific regulators controlling dormancy release in rice remain largely unknown. Here, we identified OsS40-14, a member of the senescence-associated S40 gene family, as a negative regulator of seed germination in rice. Using CRISPR/Cas9 gene editing and transgenic overexpression (OE) lines, we found that the oss40-14 mutant lines exhibit preharvest sprouting (PHS) and accelerated germination, while the OE-OsS40-14 lines show delayed germination. Notably, dormancy-breaking treatments largely eliminated these phenotypic differences. Analysis of endogenous abscisic acid (ABA) levels and the response to exogenous ABA revealed that OsS40-14 regulates germination by modulating ABA signaling. Transcriptomic and Cleavage under targets and tagmentation (CUT & Tag)-seq analyses demonstrated that OsS40-14 directly represses the transcription of two ABA catabolic genes, OsABA8ox1 and OsABA8ox3, thereby preventing the reduction of ABA levels during seed dormancy release. These findings establish OsS40-14 as a key regulator of seed germination through its control of ABA homeostasis, providing potential targets for breeding PHS-resistant varieties. OsS40-14 negatively regulates rice seed germination by directly repressing abscisic acid (ABA) catabolic genes to maintain ABA levels, thereby preventing dormancy release and enhancing preharvest sprouting resistance.
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| GB/T 7714 | Zheng, Xiangzi , Wang, Weiqi , Fan, Chunlan et al. OsS40-14 regulates rice seed germination by modulating ABA responsiveness and inhibiting dormancy release [J]. | PLANT PHYSIOLOGY , 2025 , 199 (2) . |
| MLA | Zheng, Xiangzi et al. "OsS40-14 regulates rice seed germination by modulating ABA responsiveness and inhibiting dormancy release" . | PLANT PHYSIOLOGY 199 . 2 (2025) . |
| APA | Zheng, Xiangzi , Wang, Weiqi , Fan, Chunlan , Tan, Min , Dai, Huiling , Liao, Dongmei et al. OsS40-14 regulates rice seed germination by modulating ABA responsiveness and inhibiting dormancy release . | PLANT PHYSIOLOGY , 2025 , 199 (2) . |
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Plant senescence is an integrated programme of plant development that aims to remobilize nutrients and energy from senescing tissues to developing organs under developmental and stress-induced conditions. Upstream in the regulatory network, a small family of single-stranded DNA/RNA-binding proteins known as WHIRLYs occupy a central node, acting at multiple regulatory levels and via trans-localization between the nucleus and organelles. In this review, we summarize the current progress on the role of WHIRLY members in plant development and stress-induced senescence. WHIRLY proteins can be traced back in evolution to green algae. WHIRLY proteins trade off the balance of plant developmental senescence and stress-induced senescence through maintaining organelle genome stability via R-loop homeostasis, repressing the transcription at a configuration condition, and recruiting RNA to impact organelle RNA editing and splicing, as evidenced in several species. WHIRLY proteins also act as retrograde signal transducers between organelles and the nucleus through protein modification and stromule or vesicle trafficking. In addition, WHIRLY proteins interact with hormones, reactive oxygen species and environmental signals to orchestrate cell fate in an age-dependent manner. Finally, prospects for further research and promotion to improve crop production under environmental constraints are highlighted.
Keyword ：
Evolutionary Evolutionary organelle-nucleus retrograde signals organelle-nucleus retrograde signals plant developmental senescence plant developmental senescence regulatory mechanism regulatory mechanism stress-induced senescence stress-induced senescence WHIRLY family WHIRLY family
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| GB/T 7714 | Lin, Wenfang , Huang, Dongmei , Li, Mengsi et al. WHIRLY proteins, multi-layer regulators linking the nucleus and organelles in developmental and stress-induced senescence of plants [J]. | ANNALS OF BOTANY , 2024 , 134 (4) : 521-536 . |
| MLA | Lin, Wenfang et al. "WHIRLY proteins, multi-layer regulators linking the nucleus and organelles in developmental and stress-induced senescence of plants" . | ANNALS OF BOTANY 134 . 4 (2024) : 521-536 . |
| APA | Lin, Wenfang , Huang, Dongmei , Li, Mengsi , Ren, Yujun , Zheng, Xiangzi , Wu, Binghua et al. WHIRLY proteins, multi-layer regulators linking the nucleus and organelles in developmental and stress-induced senescence of plants . | ANNALS OF BOTANY , 2024 , 134 (4) , 521-536 . |
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Background Aquaporins (AQPs) facilitate water diffusion across biological membranes and are involved in all phases of growth and development. Small and basic intrinsic proteins (SIPs) belong to the fourth subfamily of the plant AQPs. Although SIPs are widely present in higher plants, reports on SIPs are limited. Rice is one of the major food crops in the world, and water use is an important factor affecting rice growth and development; therefore, this study aimed to provide information relevant to the function and environmental response of the rice SIP gene family. Results The rice (Oryza sativa L. japonica) genome encodes two SIP-like genes, OsSIP1 and OsSIP2, whose products are predominantly located in the endoplasmic reticulum (ER) membrane but transient localization to the plasma membrane is not excluded. Heterologous expression in a yeast aquaglyceroporin-mutant fps Delta showed that both OsSIP1 and OsSIP2 made the cell more sensitive to KCl, sorbitol and H2O2, indicating facilitated permeation of water and hydrogen peroxide. In addition, the yeast cells expressing OsSIP2 were unable to efflux the toxic methylamine taken up by the endogenous MEP permeases, but OsSIP1 showed subtle permeability to methylamine, suggesting that OsSIP1 may have a wider conducting pore than OsSIP2. Expression profiling in different rice tissues or organs revealed that OsSIP1 was expressed in all tissues tested, whereas OsSIP2 was preferentially expressed in anthers and weakly expressed in other tissues. Consistent with this, histochemical staining of tissues expressing the promoter-beta-glucuronidase fusion genes revealed their tissue-specific expression profile. In rice seedlings, both OsSIPs were upregulated to varied levels under different stress conditions, including osmotic shock, high salinity, unfavorable temperature, redox challenge and pathogen attack, as well as by hormonal treatments such as GA, ABA, MeJA, SA. However, a reduced expression of both OsSIPs was observed under dehydration treatment. Conclusions Our results suggest that SIP-like aquaporins are not restricted to the ER membrane and are likely to be involved in unique membrane functions in substrate transport, growth and development, and environmental response.
Keyword ：
AQPs AQPs Endoplasmic reticulum Endoplasmic reticulum Expression profiling Expression profiling OsSIPs OsSIPs Rice Rice Stress responses Stress responses Transport Transport
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| GB/T 7714 | Miao, Miao , Shi, Ximiao , Zheng, Xiangzi et al. Characterization of SIPs-type aquaporins and their roles in response to environmental cues in rice (Oryza sativa L.) [J]. | BMC PLANT BIOLOGY , 2024 , 24 (1) . |
| MLA | Miao, Miao et al. "Characterization of SIPs-type aquaporins and their roles in response to environmental cues in rice (Oryza sativa L.)" . | BMC PLANT BIOLOGY 24 . 1 (2024) . |
| APA | Miao, Miao , Shi, Ximiao , Zheng, Xiangzi , Wu, Binghua , Miao, Ying . Characterization of SIPs-type aquaporins and their roles in response to environmental cues in rice (Oryza sativa L.) . | BMC PLANT BIOLOGY , 2024 , 24 (1) . |
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Cross talking between natural senescence and cell death in response to pathogen attack is an interesting topic; however, its action mechanism is kept open. In this study, 33 OsWRKY genes were obtained by screening with leaf aging procedure through RNA-seq dataset, and 11 of them were confirmed a significant altered expression level in the flag leaves during aging by using the reverse transcript quantitative PCR (RT-qPCR). Among them, the OsWRKY2, OsWRKY14, OsWRKY26, OsWRKY69, and OsWRKY93 members exhibited short-term alteration in transcriptional levels in response to Magnaporthe grisea infection. The CRISPR/Cas9-edited mutants of five genes were developed and confirmed, and a significant sensitivity to M. oryzae infection was observed in CRISPR OsWRKY93-edited lines; on the other hand, a significant resistance to M. oryzae infection was shown in the enhanced expression OsWRKY93 plants compared to mock plants; however, enhanced expression of other four genes have no significant affection. Interestingly, ROS accumulation was also increased in OsWRKY93 enhanced plants after flg22 treatment, compared with the controls, suggesting that OsWRKY93 is involved in PAMP-triggered immune response in rice. It indicated that OsWRKY93 was involved in both flag leaf senescence and in response to fungi attack.
Keyword ：
biotic stress biotic stress flag leaf flag leaf OsWRKY93 OsWRKY93 rice rice senescence senescence
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| GB/T 7714 | Li, Yanyun , Liao, Shuting , Mei, Pengying et al. OsWRKY93 Dually Functions Between Leaf Senescence and in Response to Biotic Stress in Rice [J]. | FRONTIERS IN PLANT SCIENCE , 2021 , 12 . |
| MLA | Li, Yanyun et al. "OsWRKY93 Dually Functions Between Leaf Senescence and in Response to Biotic Stress in Rice" . | FRONTIERS IN PLANT SCIENCE 12 (2021) . |
| APA | Li, Yanyun , Liao, Shuting , Mei, Pengying , Pan, Yueyun , Zhang, Yu , Zheng, Xiangzi et al. OsWRKY93 Dually Functions Between Leaf Senescence and in Response to Biotic Stress in Rice . | FRONTIERS IN PLANT SCIENCE , 2021 , 12 . |
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Leaf senescence, as an integral part of the final development stage for plants, primarily remobilizes nutrients from the sources to the sinks in response to different stressors. The premature senescence of leaves is a critical challenge that causes significant economic losses in terms of crop yields. Although low light causes losses of up to 50% and affects rice yield and quality, its regulatory mechanisms remain poorly elucidated. Darkness-mediated premature leaf senescence is a well-studied stressor. It initiates the expression of senescence-associated genes (SAGs), which have been implicated in chlorophyll breakdown and degradation. The molecular and biochemical regulatory mechanisms of premature leaf senescence show significant levels of redundant biomass in complex pathways. Thus, clarifying the regulatory mechanisms of low-light/dark-induced senescence may be conducive to developing strategies for rice crop improvement. This review describes the recent molecular regulatory mechanisms associated with low-light response and dark-induced senescence (DIS), and their effects on plastid signaling and photosynthesis-mediated processes, chloroplast and protein degradation, as well as hormonal and transcriptional regulation in rice.
Keyword ：
chlorophyll degradation chlorophyll degradation dark-induced senescence dark-induced senescence leaf senescence leaf senescence low light low light phytohormone phytohormone senescence-associated genes senescence-associated genes
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| GB/T 7714 | Gad, Ahmed G. , Habiba , Zheng, Xiangzi et al. Low Light/Darkness as Stressors of Multifactor-Induced Senescence in Rice Plants [J]. | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES , 2021 , 22 (8) . |
| MLA | Gad, Ahmed G. et al. "Low Light/Darkness as Stressors of Multifactor-Induced Senescence in Rice Plants" . | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 22 . 8 (2021) . |
| APA | Gad, Ahmed G. , Habiba , Zheng, Xiangzi , Miao, Ying . Low Light/Darkness as Stressors of Multifactor-Induced Senescence in Rice Plants . | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES , 2021 , 22 (8) . |
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