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学者姓名：金文松
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Abstract ：
Lyophyllum decastes is a type of edible and medicinal mushroom with high nutritional value. However, it can be infected by fungi during the fruiting process, which impairs the development of the industry. In this study, one pathogenic fungus was isolated from the diseased fruiting bodies of L. decastes. Morphological and molecular identification clarified that the causal agent of the disease was Cladobotryum mycophilum. Koch's postulates showed that C. mycophilum could infect L. decastes, and seriously affect mushroom yields. The biological properties of C. mycophilum were investigated. The data showed that the most suitable temperature for the growth of C. mycophilum was 25 degrees C with a mean growth rate of 21.45 mm d- 1 on PDA. C. mycophilum was better suited to growing in neutral or slightly acidic environments. The suitable carbon source and nitrogen source for C. mycophilum growth were lactose and peptone or yeast extract respectively. The IC50s (Half maximal inhibitory concentration) values of carbendazim and prochloraz for inhibiting C. mycophilum are respectively 1.244 mu g.mL- 1 and 3.323 mu g.mL- 1. 10 mu g.mL- 1 carbendazim can effectively control C. mycophilum during the L. decastes fruiting process. The results of this study can provide knowledge for the control of cobweb disease during the L. decastes fruiting process.
Keyword ：
Cladobotryum mycophilum Cladobotryum mycophilum Cobweb disease control Cobweb disease control Fruiting process Fruiting process Identification Identification Lyophyllum decastes Lyophyllum decastes
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| GB/T 7714 | Wu, Taorui , Chen, Youlong , Zhang, Wenxin et al. Identification, biological characterization of Cladobotryum mycophilum and its control during the fruiting process of Lyophyllum decastes [J]. | ARCHIVES OF MICROBIOLOGY , 2025 , 207 (2) . |
| MLA | Wu, Taorui et al. "Identification, biological characterization of Cladobotryum mycophilum and its control during the fruiting process of Lyophyllum decastes" . | ARCHIVES OF MICROBIOLOGY 207 . 2 (2025) . |
| APA | Wu, Taorui , Chen, Youlong , Zhang, Wenxin , Pu, Zijian , Li, Jianhua , Xu, Yawen et al. Identification, biological characterization of Cladobotryum mycophilum and its control during the fruiting process of Lyophyllum decastes . | ARCHIVES OF MICROBIOLOGY , 2025 , 207 (2) . |
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Two main adenoviral diseases have been described in pigeons: pigeon adenovirus type 1 (PiAdV-1) and pigeon adenovirus type 2 (PiAdV-2), which belong to the genus Aviadenovirus under the family Adenoviridae. PiAdV-1 and PiAdV-2 are highly pathogenic to pigeons, leading to considerable losses worldwide. To date, there is little information on the epidemiological distribution of PiAdV-1 and PiAdV-2 in pigeons due to the lack of detection and differentiation platforms for these two viruses. High-resolution melting technology (HRM) has been widely used for developing detection and differentiation platforms, with the melting profile based on the GC content in the real-time PCR (qPCR-HRM) system. This study designed and synthesized a pair of specific primers on the basis of the characteristic variations of the 52K genes of PiAdV-1 and PiAdV-2, then the detection and differentiation qPCR-HRM platform was established after conditional optimization. The results showed that this method had good specificity; it could only specifically detect PiAdV-1 and PiAdV-2, with no cross-reaction with other pigeon-origin pathogens that occur in pigeons. This method had high sensitivity, with the lowest detection limits at 57 copies/mu L (for PiAdV-1) and 56 copies/mu L (for PiAdV-2). This method had good intra-group and inter-group coefficients of variation, both of which were less than 1.5%. Field samples for the epidemiological surveillance and investigation data of PiAdV-1 and PiAdV-2 were checked. We found only PiAdV-2-positive samples in meat pigeons, but the percentages of PiAdV-1-positive, PiAdV-2-positive, and coinfection-positive samples among the racing pigeons were 5.71%, 14.29%, and 2.86%, respectively. To our knowledge, this is the first report for the simultaneous detection and differentiation of PiAdV-1 and PiAdV-2 using the qPCR-HRM platform. Our study also provided evidence of PiAdV-1 and PiAdV-2 coinfection in racing pigeons, but further studies are needed.
Keyword ：
coinfection coinfection detection and differentiation detection and differentiation epidemiological investigation epidemiological investigation PiAdV-1 PiAdV-1 PiAdV-2 PiAdV-2 qPCR-HRM platform qPCR-HRM platform
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| GB/T 7714 | Chen, Shuyu , Zhang, Wenyu , Tang, Zhiwang et al. The Detection and Differentiation of Pigeon Adenovirus Types 1 and 2 via a High-Resolution Melting Curve Platform [J]. | MICROORGANISMS , 2025 , 13 (6) . |
| MLA | Chen, Shuyu et al. "The Detection and Differentiation of Pigeon Adenovirus Types 1 and 2 via a High-Resolution Melting Curve Platform" . | MICROORGANISMS 13 . 6 (2025) . |
| APA | Chen, Shuyu , Zhang, Wenyu , Tang, Zhiwang , Lu, Tingting , Wan, Chunhe , Jin, Wensong et al. The Detection and Differentiation of Pigeon Adenovirus Types 1 and 2 via a High-Resolution Melting Curve Platform . | MICROORGANISMS , 2025 , 13 (6) . |
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Microscopic examination is commonly employed to assess edible fungal mycelium vitality. However, this method can become time-intensive when evaluating a substantial volume of hyphae samples, which implies an urgent need to develop an accurate and automatic determination method. The challenges of mycelium detection come mostly from the multi-scale target detection under various magnifications. In this study, microscopic images of 10 edible fungi strains under different magnification scales or stain colors were collected to create a dataset. An improved multi-scale object detection model for mycelium vitality detection, CCHA YOLO, was proposed by enhancing the Backbone via combining Yolov8m and Swin Transformer (SwinT). Meanwhile, the Convolutional Block Attention Module (CBAM) was introduced to the Head, as well as optimized post -processing techniques to further promote model performance. The results indicated that CCHA YOLO achieved a mAP 50:95 (mean average precision) of 89.02 % with a computational load of 98.61 GFLOPs. Additionally, it indicates a 16.67 % accuracy enhancement, needing only 11.3 more computational operations compared to the baseline YOLOv8m. In the meantime, CCHA YOLO was deployed on the web-based edge to facilitate the detection of microscopic images, highlighting the practical applicability of CCHA YOLO in determining mycelium vitality.
Keyword ：
Clamp connection (CC) Clamp connection (CC) Hybrid Swim Transformer Hybrid Swim Transformer Hyphae autolysis (HA) Hyphae autolysis (HA) Multi-scale object detection Multi-scale object detection Web deployment Web deployment
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| GB/T 7714 | Wu, Libin , Lin, Shaodan , Jin, Wensong et al. CCHA YOLO for mycelium clamp connection (CC) and hyphae Autolysis (HA) detection under microscopy imaging and web deployment [J]. | MICROCHEMICAL JOURNAL , 2024 , 201 . |
| MLA | Wu, Libin et al. "CCHA YOLO for mycelium clamp connection (CC) and hyphae Autolysis (HA) detection under microscopy imaging and web deployment" . | MICROCHEMICAL JOURNAL 201 (2024) . |
| APA | Wu, Libin , Lin, Shaodan , Jin, Wensong , Weng, Haiyong , Xu, Jinchai , Zhang, Lintong et al. CCHA YOLO for mycelium clamp connection (CC) and hyphae Autolysis (HA) detection under microscopy imaging and web deployment . | MICROCHEMICAL JOURNAL , 2024 , 201 . |
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The homologous recombination strategy has a long history of editing Saccharomyces cerevisiae target genes. The application of CRISPR/Cas9 strategy to editing target genes in S. cerevisiae has also received a lot of attention in recent years. All findings seem to indicate that editing relevant target genes in S. cerevisiae is an extremely easy event. In this study, we systematically analyzed the advantages and disadvantages of homologous recombination (HR) strategy, CRISPR/Cas9 strategy, and CRISPR/Cas9 combined homology-mediated repair (CRISPR/Case9HDR) strategy in knocking out BY4742 ade2. Our data showed that when the ade2 was knocked out by HR strategy, a large number of clones appeared to be off-target, and 10 %-80 % of the so-called knockout clones obtained were heteroclones. When the CRISPR/Cas9 strategy was applied, 60% of clones were off-target and the rest were all heteroclones. Interestingly, most of the cells were edited successfully, but at least 60 % of the clones were heteroclones, when the CRISPR/Cas9-HDR strategy was employed. Our results clearly showed that the emergence of heteroclone seems inevitable regardless of the strategies used for editing BY4742 ade2. Given the characteristics of BY4742 defective in ade2 showing red on the YPD plate, we attempted to build an efficient yeast gene editing strategy, in which the CRISPR/Cas9 combines homology-mediated repair template carrying an ade2 expression cassette, BY4742(ade2 Delta 0) as the start strain. We used this strategy to successfully achieve 100 % knockout efficiency of trp1, indicating that technical challenges of how to easily screen out pure knockout clones without color phenotype have been solved. Our data showed in this study not only establishes an efficient yeast gene knockout strategy with dual auxotrophy coupled red labeling but also provides new ideas and references for the knockout of target genes in the monokaryotic mycelium of macrofungi.
Keyword ：
ade2 ade2 CRISPR/Cas9 CRISPR/Cas9 Homologous recombination Homologous recombination Homology-mediated repair Homology-mediated repair Saccharomyces cerevisiae Saccharomyces cerevisiae
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| GB/T 7714 | Li, Jianhua , Wu, Taorui , Wang, Jialong et al. Dual auxotrophy coupled red labeling strategy for efficient genome editing in Saccharomyces cerevisiae [J]. | FUNGAL GENETICS AND BIOLOGY , 2024 , 173 . |
| MLA | Li, Jianhua et al. "Dual auxotrophy coupled red labeling strategy for efficient genome editing in Saccharomyces cerevisiae" . | FUNGAL GENETICS AND BIOLOGY 173 (2024) . |
| APA | Li, Jianhua , Wu, Taorui , Wang, Jialong , Chen, Youlong , Zhang, Wenxin , Cai, Lijun et al. Dual auxotrophy coupled red labeling strategy for efficient genome editing in Saccharomyces cerevisiae . | FUNGAL GENETICS AND BIOLOGY , 2024 , 173 . |
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本发明提出一种基于机器视觉的液体菌种生物量的软测量方法，包括：离线采样并解析全发酵周期内液体菌种生物量参数及其演变规律；手工测量的辅助变量特征M‑Features建立生物量参数软测量模型M1；采集不同发酵阶段的液体菌种的可见光图像与可见‑近红外I区‑近红外II区多源波谱数据，构建图谱数据集，搭建多任务机器视觉模型V1用于提取基于机器视觉辅助变量特征E‑Features；联合生物量参数软测量模型M1及多任务机器视觉模型V1，建立E‑Features与M‑Features的关联，得到级联机器视觉软测量模型MV；部署所述MV模型于终端嵌入式智能设备，并设计任务执行的交互软件。该方法可在线实时评估液体菌种发酵过程生物量参数演变的趋势，以指导菌种的投产应用。
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| GB/T 7714 | 叶大鹏 , 武礼宾 , 翁海勇 et al. 一种基于机器视觉的液体菌种生物量的软测量方法 : CN202410596991.8[P]. | 2024-05-14 . |
| MLA | 叶大鹏 et al. "一种基于机器视觉的液体菌种生物量的软测量方法" : CN202410596991.8. | 2024-05-14 . |
| APA | 叶大鹏 , 武礼宾 , 翁海勇 , 孙淑静 , 金文松 , 林铃 . 一种基于机器视觉的液体菌种生物量的软测量方法 : CN202410596991.8. | 2024-05-14 . |
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本实用新型公开了一种食用菌培育霉变喷药装置，主罐体，所述主罐体下方设置有喷药头；转动安装在所述主罐体侧下方的若干个活动杆；所述活动杆包括第一弯折部、第二弯折部、以及一端连接所述第一弯折部下端的连接部，所述连接部另一端与所述第二弯折部固定连接，通过喷药头对正下方的霉变培育袋进行喷药处理。为预防喷药过程中，霉菌孢子受到冲击造成飞散外溢的情况，设置了套膜将主罐体以下部分包覆，形成只有下方开口半封闭的喷药区，而为进一步加强套膜与活动杆的固定效果，故在所述第二弯折部与连接部的侧面设有若干个通孔，所述通孔与套膜内侧缝接固定。
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| GB/T 7714 | 金文松 , 胡开辉 , 孙淑静 et al. 一种食用菌培育霉变喷药装置 : CN202321892039.X[P]. | 2023-07-18 . |
| MLA | 金文松 et al. "一种食用菌培育霉变喷药装置" : CN202321892039.X. | 2023-07-18 . |
| APA | 金文松 , 胡开辉 , 孙淑静 , 李佳欢 . 一种食用菌培育霉变喷药装置 : CN202321892039.X. | 2023-07-18 . |
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本发明公开了一种海鲜菇酱油制备方法，其步骤包括：S1，选取新鲜海鲜菇为原料，清洗后，按料液比1：4比例加水，置于负压提取罐中在温度区间温度70‑80℃内进行提取，且过滤掉海鲜菇的残留碎屑及杂质，获得海鲜菇提取液；S2，将海鲜菇提取液按照水体体积20‑30％，置于温度区间为50～55℃水中稀释，本发明以海鲜菇粉替代大豆致敏物豆粕，解决了豆制品过敏人群食用酱油会引起过敏反应等问题；通过梯度变温法烘干海鲜菇，同时通过烘干提升海鲜菇风味。结合采用先高温后中温的变温发酵工艺，显著改善了低盐固态酱油产品的风味，提高了其香味、营养价值，既方便了对豆类产品过敏人群的食用，也对其他无过敏人群提供了一种可供选择的风味酱油。
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| GB/T 7714 | 胡开辉 , 李佳欢 , 金文松 et al. 一种海鲜菇酱油制备方法 : CN202311841688.1[P]. | 2023-12-28 . |
| MLA | 胡开辉 et al. "一种海鲜菇酱油制备方法" : CN202311841688.1. | 2023-12-28 . |
| APA | 胡开辉 , 李佳欢 , 金文松 , 孙淑静 , 陈利丁 . 一种海鲜菇酱油制备方法 : CN202311841688.1. | 2023-12-28 . |
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本发明提供了基于多光谱漫反射与边缘计算的液体菌种发酵监测方法，包括以下步骤：步骤1：获取液体菌种发酵过程的关键生物参数的特征光谱信息；步骤2：根据发酵过程的特征光谱波段信息，研发多光谱漫反射成像系统；步骤3：构建可融合多光谱数据处理的轻量化的目标检测深度学习模型，便于进行嵌入式机器视觉部署；步骤4：使用级联定标法，使所述视觉模型可识别定位到发酵视窗位置，再在感兴趣区域内进行关键目标检测和分析；步骤5：将上述的深度学习视觉模型部署于智能边缘计算设备，实时对发酵过程图谱信息进行处理。应用本技术方案可实现嵌入式机器视觉在发酵工业现场的应用。
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| GB/T 7714 | 武礼宾 , 叶大鹏 , 翁海勇 et al. 基于多光谱漫反射与边缘计算的液体菌种发酵监测方法 : CN202311113428.2[P]. | 2023-08-31 . |
| MLA | 武礼宾 et al. "基于多光谱漫反射与边缘计算的液体菌种发酵监测方法" : CN202311113428.2. | 2023-08-31 . |
| APA | 武礼宾 , 叶大鹏 , 翁海勇 , 孙淑静 , 金文松 , 林铃 et al. 基于多光谱漫反射与边缘计算的液体菌种发酵监测方法 : CN202311113428.2. | 2023-08-31 . |
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本发明公开了一种银耳菌株、InDel标记及引物和鉴定方法，该银耳菌株命名为TWH‑SD2，于2023年04月07日保藏于中国典型培养物保藏中心，保藏编号为CCTCC NO：M 2023495，保藏地址为中国.武汉.武汉大学。本发明提供的新品种TWH‑SD2在口感上表现为更加Q弹且在产品运输过程中可以更好地抵御机械力压迫导致的形变，子实体颜色雪白，生产周期缩短3‑5天，朵型更加圆整蓬松，生物学效率高，具有很好的开发应用前景。本发明还提供了鉴定上述新品种TWH‑SD2的InDel标记、引物和鉴定方法，能够很好的将福银雪耳TWH‑SD2与其他银耳品种区分开来。
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| GB/T 7714 | 孙淑静 , 张琪辉 , 谢家成 et al. 一种银耳菌株、InDel标记及引物和鉴定方法 : CN202310670753.2[P]. | 2023-06-07 . |
| MLA | 孙淑静 et al. "一种银耳菌株、InDel标记及引物和鉴定方法" : CN202310670753.2. | 2023-06-07 . |
| APA | 孙淑静 , 张琪辉 , 谢家成 , 李佳欢 , 金文松 . 一种银耳菌株、InDel标记及引物和鉴定方法 : CN202310670753.2. | 2023-06-07 . |
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本发明公开了一种鹿茸菇液体菌种培养基及采用该培养基栽培鹿茸菇的方法，鹿茸菇液体菌种培养基，包括以下重量份的组分：全麦粉40‑60份、花生饼粉18‑26份、K2HPO41.5‑2.5份、MgSO4·7H2O 1.5‑2.5份。采用上述鹿茸菇液体菌种培养基栽培鹿茸菇的方法，包括以下步骤：制备鹿茸菇一级种子液，然后采用上述鹿茸菇液体菌种培养基对一级种子液进行发酵培养，得到发酵液，将发酵液接种到栽培料中进行出菇培养。本发明提供的鹿茸菇液体菌种培养基中全麦粉可为鹿茸菇提供碳源，花生饼粉为鹿茸菇提供氮源，K2HPO4和MgSO4·7H2O为鹿茸菇提供无机盐，培养基成本低，可有效提高鹿茸菇的菌丝生物量。
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| GB/T 7714 | 金文松 , 胡开辉 , 孙淑静 et al. 一种鹿茸菇液体菌种培养基及采用该培养基栽培鹿茸菇的方法 : CN202211224385.0[P]. | 2022-10-08 . |
| MLA | 金文松 et al. "一种鹿茸菇液体菌种培养基及采用该培养基栽培鹿茸菇的方法" : CN202211224385.0. | 2022-10-08 . |
| APA | 金文松 , 胡开辉 , 孙淑静 , 李佳欢 , 单灿灿 . 一种鹿茸菇液体菌种培养基及采用该培养基栽培鹿茸菇的方法 : CN202211224385.0. | 2022-10-08 . |
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