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学者姓名：周洁
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Abstract ：
Fusarium graminearum is an important plant pathogenic fungus that causes disease and yield reduction in many cereal crops, such as wheat and barley. Gyp8 stimulates GTP hydrolysis on Ypt1 in yeast. However, the functions of Gyp8 in plant pathogenic fungi are still unknown. In this study, we investigated the roles of FgGyp8 in F. graminearum genetic and pathological analyses. Through gene knockout and phenotypic analyses, we found that FgGyp8 is required for vegetative growth in F. graminearum. The conidiation, conidial size and number of septa per conidium of Delta Fggyp8 mutant are significantly reduced when compared to the wild type PH-1. Furthermore, FgGyp8 is crucial for pathogenicity on wheat coleoptiles and wheat heads. FgGyp8 contains a conserved TBC domain. Domain deletion analysis showed that the TBC domain, C-and N-terminal regions of FgGyp8 are all important for its biological functions in F. graminearum. Moreover, we showed that FgGyp8 catalyzes the hydrolysis of the GTP on FgRab1 to GDP in vitro, indicating that FgGyp8 is a GTPase-activating protein (GAP) for FgRab1. In addition, we demonstrated that FgGyp8 is required for FgSnc1-mediated fusion of secretory vesicles with the plasma membrane in F. graminearum. Finally, we showed that FgGyp8 has functional redundancy with another FgRab1 GAP, FgGyp1, in F. graminearum. Taken together, we conclude that FgGyp8 is required for vegetative growth, conidiogenesis, pathogenicity and acts as a GAP for FgRab1 F. graminearum.
Keyword ：
conidiogenesis conidiogenesis FgGyp8 FgGyp8 FgRab1 FgRab1 Fusarium graminearum Fusarium graminearum GTPase-activating protein GTPase-activating protein pathogenicity pathogenicity
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| GB/T 7714 | Zhang, Xing-zhi , Chen, Shuang , Abubakar, Yakubu Saddeeq et al. FgGyp8 as a putative FgRab1 GAP is required for growth and pathogenesis by regulating FgSnc1-mediated secretory vesicles fusion in Fusarium graminearum [J]. | JOURNAL OF INTEGRATIVE AGRICULTURE , 2023 , 22 (11) : 3444-3457 . |
| MLA | Zhang, Xing-zhi et al. "FgGyp8 as a putative FgRab1 GAP is required for growth and pathogenesis by regulating FgSnc1-mediated secretory vesicles fusion in Fusarium graminearum" . | JOURNAL OF INTEGRATIVE AGRICULTURE 22 . 11 (2023) : 3444-3457 . |
| APA | Zhang, Xing-zhi , Chen, Shuang , Abubakar, Yakubu Saddeeq , Mao, Xu-zhao , Miao, Peng-fei , Wang, Zong-hua et al. FgGyp8 as a putative FgRab1 GAP is required for growth and pathogenesis by regulating FgSnc1-mediated secretory vesicles fusion in Fusarium graminearum . | JOURNAL OF INTEGRATIVE AGRICULTURE , 2023 , 22 (11) , 3444-3457 . |
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The AP1 complex is a highly conserved clathrin adaptor that plays important roles in regulating cargo protein sorting and intracellular vesicle trafficking in eukaryotes. However, the functions of the AP1 complex in the plant pathogenic fungi including the devastating wheat pathogen Fusarium graminearum are still unclear. In this study, we investigated the biological functions of FgAP1(sigma), a subunit of the AP1 complex in F. graminearum. Disruption of FgAP1(sigma) causes seriously impaired fungal vegetative growth, conidiogenesis, sexual development, pathogenesis, and deoxynivalenol (DON) production. The delta Fgap1(sigma) mutants were found to be less sensitive to KCl- and sorbitol-induced osmotic stresses but more sensitive to SDS-induced stress than the wild-type PH-1. Although the growth inhibition rate of the delta Fgap1(sigma) mutants was not significantly changed under calcofluor white (CFW) and Congo red (CR) stresses, the protoplasts released from delta Fgap1(sigma) hyphae were decreased compared with the wild-type PH-1, suggesting that FgAP1(sigma) is necessary for cell wall integrity and osmotic stresses in F. graminearum. Subcellular localization assays showed that FgAP1(sigma) was predominantly localized to endosomes and the Golgi apparatus. In addition, FgAP1(beta)-GFP, FgAP1(gamma)-GFP, and FgAP1(mu)-GFP also localize to the Golgi apparatus. FgAP1(beta) interacts with FgAP1(sigma), FgAP1(gamma), and FgAP1(mu), while FgAP1(sigma) regulates the expression of FgAP1(beta), FgAP1(gamma), and FgAP1(mu) in F. graminearum. Furthermore, the loss of FgAP1(sigma) blocks the transportation of the v-SNARE protein FgSnc1 from the Golgi to the plasma membrane and delays the internalization of FM4-64 dye into the vacuole. Taken together, our results demonstrate that FgAP1(sigma) plays vital roles in vegetative growth, conidiogenesis, sexual reproduction, DON production, pathogenicity, cell wall integrity, osmotic stress, exocytosis, and endocytosis in F. graminearum. These findings unveil the functions of the AP1 complex in filamentous fungi, most notably in F. graminearum, and lay solid foundations for effective prevention and control of Fusarium head blight (FHB).
Keyword ：
AP1 complex AP1 complex FgAP1(sigma) FgAP1(sigma) Fusarium graminearum Fusarium graminearum wheat scab wheat scab
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| GB/T 7714 | Wu, Congxian , Chen, Huilin , Yuan, Mingyue et al. FgAP1s Is Critical for Vegetative Growth, Conidiation, Virulence, and DON Biosynthesis in Fusarium graminearum [J]. | JOURNAL OF FUNGI , 2023 , 9 (2) . |
| MLA | Wu, Congxian et al. "FgAP1s Is Critical for Vegetative Growth, Conidiation, Virulence, and DON Biosynthesis in Fusarium graminearum" . | JOURNAL OF FUNGI 9 . 2 (2023) . |
| APA | Wu, Congxian , Chen, Huilin , Yuan, Mingyue , Zhang, Meiru , Abubakar, Yakubu Saddeeq , Chen, Xin et al. FgAP1s Is Critical for Vegetative Growth, Conidiation, Virulence, and DON Biosynthesis in Fusarium graminearum . | JOURNAL OF FUNGI , 2023 , 9 (2) . |
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The RNA exosome complex is a conserved, multisubunit RNase complex that contributes to the processing and degradation of RNAs in mammalian cells. However, the roles of the RNA exosome in phytopathogenic fungi and how it relates to fungal development and pathogenicity remain unclear. Herein, we identified 12 components of the RNA exosome in the wheat fungal pathogen Fusarium graminearum. Live-cell imaging showed that all the components of the RNA exosome complex are localized in the nucleus. FgEXOSC1 and FgEXOSCA were successfully knocked out; they are both involved in the vegetative growth, sexual reproduction, and pathogenicity of F. graminearum. Moreover, deletion of FgEXOSC1 resulted in abnormal toxisomes, decreased deoxynivalenol (DON) production, and downregulation of the expression levels of DON biosynthesis genes. The RNA-binding domain and N-terminal region of FgExosc1 are required for its normal localization and functions. Transcriptome sequencing (RNA-seq) showed that the disruption of FgEXOSC1 resulted in differential expression of 3,439 genes. Genes involved in processing of noncoding RNA (ncRNA), rRNA and ncRNA metabolism, ribosome biogenesis, and ribonucleoprotein complex biogenesis were significantly upregulated. Furthermore, subcellular localization, green fluorescent protein (GFP) pulldown, and coimmunoprecipitation (co-IP) assays demonstrated that FgExosc1 associates with the other components of the RNA exosome to form the RNA exosome complex in F. graminearum. Deletion of FgEXOSC1 and FgEXOSCA reduced the relative expression of some of the other subunits of the RNA exosome. Deletion of FgEXOSC1 affected the localization of FgExosc4, FgExosc6, and FgExosc7. In summary, our study reveals that the RNA exosome is involved in vegetative growth, sexual reproduction, DON production, and pathogenicity of F. graminearum.IMPORTANCE The RNA exosome complex is the most versatile RNA degradation machinery in eukaryotes. However, little is known about how this complex regulates the development and pathogenicity of plant-pathogenic fungi. In this study, we systematically identified 12 components of the RNA exosome complex in Fusarium head blight fungus Fusarium graminearum and first unveiled their subcellular localizations and established their biological functions in relation to the fungal development and pathogenesis. All the RNA exosome components are localized in the nucleus. FgExosc1 and FgExoscA are both required for the vegetative growth, sexual reproduction, DON production and pathogenicity in F. graminearum. FgExosc1 is involved in ncRNA processing, rRNA and ncRNA metabolism process, ribosome biogenesis and ribonucleoprotein complex biogenesis. FgExosc1 associates with the other components of RNA exosome complex and form the exosome complex in F. graminearum. Our study provides new insights into the role of the RNA exosome in regulating RNA metabolism, which is associated with fungal development and pathogenicity. The RNA exosome complex is the most versatile RNA degradation machinery in eukaryotes. However, little is known about how this complex regulates the development and pathogenicity of plant-pathogenic fungi.
Keyword ：
DON DON Fusarium graminearum Fusarium graminearum pathogenicity pathogenicity RNA exosome complex RNA exosome complex RNA processing RNA processing
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| GB/T 7714 | Yuan, Yanping , Mao, Xuzhao , Abubakar, Yakubu Saddeeq et al. Genome-Wide Characterization of the RNA Exosome Complex in Relation to Growth, Development, and Pathogenicity of Fusarium graminearum [J]. | MICROBIOLOGY SPECTRUM , 2023 , 11 (3) . |
| MLA | Yuan, Yanping et al. "Genome-Wide Characterization of the RNA Exosome Complex in Relation to Growth, Development, and Pathogenicity of Fusarium graminearum" . | MICROBIOLOGY SPECTRUM 11 . 3 (2023) . |
| APA | Yuan, Yanping , Mao, Xuzhao , Abubakar, Yakubu Saddeeq , Zheng, Wenhui , Wang, Zonghua , Zhou, Jie et al. Genome-Wide Characterization of the RNA Exosome Complex in Relation to Growth, Development, and Pathogenicity of Fusarium graminearum . | MICROBIOLOGY SPECTRUM , 2023 , 11 (3) . |
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Author summaryCell cycle is controlled by numerous mechanisms ensuring correct cell division. One fundamental question in the cell cycle is the mitotic exit and entry into the next cycle, but the mechanism of mitotic exit network in the wheat head blight fungus Fusarium graminearum remains unclear. In this study, we identified a small GTPase FgTem1 to be involved in mitotic exit in this fungus and demonstrated that this protein is required for the fungal pathogenicity and functions in regulating the infection structures formation and invasive hyphal growth on wheat spikelets and wheat coleoptiles. Furthermore, the regulatory mechanisms of FgTem1 have been further investigated. To the best of our knowledge, this work is the most comprehensive genetic dissection of Tem1 in filamentous fungal pathogens. Our results unveil a key link between mitotic exit network and the pathogenicity of the phytopathogen. These findings provide a novel molecular basis for the design and development of chemicals that prevent phytopathogen infection. The mitotic exit is a key step in cell cycle, but the mechanism of mitotic exit network in the wheat head blight fungus Fusarium graminearum remains unclear. F. graminearum infects wheat spikelets and colonizes the entire head by growing through the rachis node at the bottom of each spikelet. In this study, we found that a small GTPase FgTem1 plays an important role in F. graminearum pathogenicity and functions in regulating the formation of infection structures and invasive hyphal growth on wheat spikelets and wheat coleoptiles, but plays only little roles in vegetative growth and conidiation of the phytopathogen. FgTem1 localizes to both the inner nuclear periphery and the spindle pole bodies, and negatively regulates mitotic exit in F. graminearum. Furthermore, the regulatory mechanisms of FgTem1 have been further investigated by high-throughput co-immunoprecipitation and genetic strategies. The septins FgCdc10 and FgCdc11 were demonstrated to interact with the dominant negative form of FgTem1, and FgCdc11 was found to regulate the localization of FgTem1. The cell cycle arrest protein FgBub2-FgBfa1 complex was shown to act as the GTPase-activating protein (GAP) for FgTem1. We further demonstrated that a direct interaction exists between FgBub2 and FgBfa1 which crucially promotes conidiation, pathogenicity and DON production, and negatively regulates septum formation and nuclear division in F. graminearum. Deletions of FgBUB2 and FgBFA1 genes caused fewer perithecia and immature asci formations, and dramatically down-regulated trichothecene biosynthesis (TRI) gene expressions. Double deletion of FgBUB2/FgBFA1 genes showed that FgBUB2 and FgBFA1 have little functional redundancy in F. graminearum. In summary, we systemically demonstrated that FgTem1 and its GAP FgBub2-FgBfa1 complex are required for fungal development and pathogenicity in F. graminearum.
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| GB/T 7714 | Miao, Pengfei , Mao, Xuzhao , Chen, Shuang et al. The mitotic exit mediated by small GTPase Tem1 is essential for the pathogenicity of Fusarium graminearum [J]. | PLOS PATHOGENS , 2023 , 19 (3) . |
| MLA | Miao, Pengfei et al. "The mitotic exit mediated by small GTPase Tem1 is essential for the pathogenicity of Fusarium graminearum" . | PLOS PATHOGENS 19 . 3 (2023) . |
| APA | Miao, Pengfei , Mao, Xuzhao , Chen, Shuang , Abubakar, Yakubu Saddeeq , Li, Yulong , Zheng, Wenhui et al. The mitotic exit mediated by small GTPase Tem1 is essential for the pathogenicity of Fusarium graminearum . | PLOS PATHOGENS , 2023 , 19 (3) . |
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Rab GTPases are key regulators of membrane and intracellular vesicle transports. However, the biological functions of FgRab1 are still unclear in the devastating wheat pathogen Fusarium graminearum. In this study, we generated constitutively active (CA) and dominant-negative (DN) forms of FgRAB1 from the wild-type PH-1 background for functional analyses. Phenotypic analyses of these mutants showed that FgRab1 is important for vegetative growth, cell wall integrity and hyphal branching. Compared to the PH-1 strain, the number of spores produced by the Fgrab1DN strain was significantly reduced, with obviously abnormal conidial morphology. The number of septa in the conidia of the Fgrab1DN mutant was fewer than that observed in the PH-1 conidia. Fgrab1DN was dramatically reduced in its ability to cause Fusarium head blight symptoms on wheat heads. GFP-FgRab1 was observed to partly localize to the Golgi apparatus, endoplasmic reticulum and Spitzenkorper. Furthermore, we found that FgRab1 inactivation blocks not only the transport of the v-SNARE protein FgSnc1 from the Golgi to the plasma membrane but also the fusion of endocytic vesicles with their target membranes and general autophagy. In summary, our results indicate that FgRab1 plays vital roles in vegetative growth, conidiogenesis, pathogenicity, autophagy, vesicle fusion and trafficking in F. graminearum.
Keyword ：
autophagy autophagy constitutively active constitutively active dominant negative dominant negative FgRab1 FgRab1 Fusarium graminearum Fusarium graminearum pathogenicity pathogenicity vesicle transport vesicle transport
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| GB/T 7714 | Yuan, Yanping , Zhang, Meiru , Li, Jingjing et al. The Small GTPase FgRab1 Plays Indispensable Roles in the Vegetative Growth, Vesicle Fusion, Autophagy and Pathogenicity of Fusarium graminearum [J]. | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES , 2022 , 23 (2) . |
| MLA | Yuan, Yanping et al. "The Small GTPase FgRab1 Plays Indispensable Roles in the Vegetative Growth, Vesicle Fusion, Autophagy and Pathogenicity of Fusarium graminearum" . | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 23 . 2 (2022) . |
| APA | Yuan, Yanping , Zhang, Meiru , Li, Jingjing , Yang, Chengdong , Abubakar, Yakubu Saddeeq , Chen, Xin et al. The Small GTPase FgRab1 Plays Indispensable Roles in the Vegetative Growth, Vesicle Fusion, Autophagy and Pathogenicity of Fusarium graminearum . | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES , 2022 , 23 (2) . |
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Calcium and manganese transporters play important roles in regulating Ca2+ and Mn2+ homeostasis in cells, which is necessary for the normal physiological activities of eukaryotes. Gdt1 and Pmr1 function as calcium/manganese transporters in the Golgi apparatus. However, the functions of Gdt1 and Pmr1 have not been previously characterized in the plant pathogenic fungus Fusarium graminearum. Here, we identified and characterized the biological functions of FgGdt1 and FgPmr1 in F. graminearum. Our study shows that FgGdt1 and FgPmr1 are both localized to the cis- and medial-Golgi. Disruption of FgGdt1 or FgPmr1 in F. graminearum caused serious defects in vegetative growth, conidiation, sexual development and significantly decreased virulence in wheat but increased deoxynivalenol (DON) production. Importantly, FgGdt1 is involved in Ca2+ and Mn2+ homeostasis and the severe phenotypic defects of the Delta Fggdt1 mutant were largely due to loss of FgGdt1 function in Mn2+ transportation. FgGdt1-mCherry colocalizes with FgPmr1-GFP at the Golgi, and FgGDT1 exerts its biological function upstream of FgPMR1. Taken together, our results collectively demonstrate that the cis- and medial-Golgi-localized proteins FgGdt1 and FgPmr1 regulate Ca2+ and Mn2+ homeostasis of the Golgi apparatus, and this function is important in modulating the growth, development, DON biosynthesis and pathogenicity of F. graminearum.
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| GB/T 7714 | Wu, Congxian , Guo, Zhongkun , Zhang, Meiru et al. Golgi-localized calcium/manganese transporters FgGdt1 and FgPmr1 regulate fungal development and virulence by maintaining Ca2+ and Mn2+ homeostasis in Fusarium graminearum [J]. | ENVIRONMENTAL MICROBIOLOGY , 2022 , 24 (10) : 4623-4640 . |
| MLA | Wu, Congxian et al. "Golgi-localized calcium/manganese transporters FgGdt1 and FgPmr1 regulate fungal development and virulence by maintaining Ca2+ and Mn2+ homeostasis in Fusarium graminearum" . | ENVIRONMENTAL MICROBIOLOGY 24 . 10 (2022) : 4623-4640 . |
| APA | Wu, Congxian , Guo, Zhongkun , Zhang, Meiru , Chen, Huilin , Peng, Minghui , Abubakar, Yakubu Saddeeq et al. Golgi-localized calcium/manganese transporters FgGdt1 and FgPmr1 regulate fungal development and virulence by maintaining Ca2+ and Mn2+ homeostasis in Fusarium graminearum . | ENVIRONMENTAL MICROBIOLOGY , 2022 , 24 (10) , 4623-4640 . |
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Fusarium verticillioides, a well-known fungal pathogen that causes severe disease in maize and contaminates the grains with fumonisin B1 (FB1) mycotoxin, affects the yield and quality of maize worldwide. The intrinsic roles of peroxisome targeting signal (PTS)-containing proteins in phytopathogens remain elusive. We therefore explored the regulatory role and other biological functions of the components of PTS2 receptor complex, FvPex7 and FvPex20, in F. verticillioides. We found that FvPex7 directly interacts with the carboxyl terminus of FvPex20 in F. verticillioides. PTS2-containing proteins are recognized and bound by the FvPex7 receptor or the FvPex7-Pex20 receptor complex in the cytoplasm, but the peroxisome localization of the PTS2-Pex7-Pex20 complex is only determined by Pex20 in F. verticillioides. However, we observed that some putative PTS2 proteins that interact with Pex7 are not transported into the peroxisomes, but a PTS1 protein that interacts with Pex5 was detected in the peroxisomes. Furthermore, Delta Fvpex7pex20 as well as Delta Fvpex7pex5 double mutants exhibited reduced pathogenicity and FB1 biosynthesis, along with defects in conidiation. The PTS2 receptor complex mutants (Delta Fvpex7pex20) grew slowly on minimal media and showed reduced sensitivity to cell wall and cell membrane stress-inducing agents compared to the wild type. Taken together, we conclude that the PTS2 receptor complex mediates peroxisome matrix proteins import and contributes to pathogenicity and FB1 biosynthesis in F. verticillioides.
Keyword ：
FB1 FB1 Fusarium verticillioides Fusarium verticillioides Fvpex20 Fvpex20 Fvpex7 Fvpex7 Pathogenicity Pathogenicity PTS protein PTS protein
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| GB/T 7714 | Lin, Mei , Abubakar, Yakubu Saddeeq , Wei, Lijing et al. Fusarium verticillioides Pex7/20 mediates peroxisomal PTS2 pathway import, pathogenicity, and fumonisin B1 biosynthesis [J]. | APPLIED MICROBIOLOGY AND BIOTECHNOLOGY , 2022 , 106 (19-20) : 6595-6609 . |
| MLA | Lin, Mei et al. "Fusarium verticillioides Pex7/20 mediates peroxisomal PTS2 pathway import, pathogenicity, and fumonisin B1 biosynthesis" . | APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 106 . 19-20 (2022) : 6595-6609 . |
| APA | Lin, Mei , Abubakar, Yakubu Saddeeq , Wei, Lijing , Wang, Jiajia , Lu, Xiange , Lu, Guodong et al. Fusarium verticillioides Pex7/20 mediates peroxisomal PTS2 pathway import, pathogenicity, and fumonisin B1 biosynthesis . | APPLIED MICROBIOLOGY AND BIOTECHNOLOGY , 2022 , 106 (19-20) , 6595-6609 . |
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The retromer complex, composed of the cargo-selective complex (CSC) Vps35-Vps29-Vps26 in complex with the sorting nexin dimer Vps5-Vps17, mediates the sorting and retrograde transport of cargo proteins from the endosomes to the trans-Golgi network in eukaryotic cells. Rab proteins belong to the Ras superfamily of small GTPases and regulate many trafficking events including vesicle formation, budding, transport, tethering, docking and fusion with target membranes. Herein, we investigated the potential functional relationship between the retromer complex and the 11 Rab proteins that exist in Fusarium graminearum using genetic and high-resolution laser confocal microscopic approaches. We found that only FgRab5 (FgRab5A and FgRab5B) and FgRab7 associate with the retromer complex. Both FgVps35-GFP and FgVps17-GFP are mis-localized and appear diffused in the cytoplasm of Delta Fgrab5A, Delta Fgrab5B and Delta Fgrab7 mutants as compared to their punctate localization within the endosomes of the wild-type. FgRab7 and FgRab5B were found to co-localize with the retromer on endosomal membranes. Most strikingly, we found that these three Rab GTPases are indispensable for endosome biogenesis as both early and late endosomes could not be detected in the cells of the mutants after FM4-64 staining of the cells, while they were very clearly seen in the wild-type PH-1. Furthermore, FgRab7 was found to recruit FgVps35 but not FgVps17 to the endosomal membranes, whereas FgRab5B recruits both FgVps35 and FgVps17 to the membranes. Thus, we conclude that the Rab proteins FgRab5A, FgRab5B and FgRab7 play critical roles in the biogenesis of endosomes and in regulating retromer-mediated trafficking in F. graminearum.
Keyword ：
Fusarium graminearum Fusarium graminearum Rab GTPases Rab GTPases Retromer Retromer vesicle trafficking vesicle trafficking
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| GB/T 7714 | Abubakar, Yakubu Saddeeq , Qiu, Han , Fang, Wenqin et al. FgRab5 and FgRab7 are essential for endosomes biogenesis and non-redundantly recruit the retromer complex to the endosomes in Fusarium graminearum [J]. | STRESS BIOLOGY , 2021 , 1 (1) . |
| MLA | Abubakar, Yakubu Saddeeq et al. "FgRab5 and FgRab7 are essential for endosomes biogenesis and non-redundantly recruit the retromer complex to the endosomes in Fusarium graminearum" . | STRESS BIOLOGY 1 . 1 (2021) . |
| APA | Abubakar, Yakubu Saddeeq , Qiu, Han , Fang, Wenqin , Zheng, Huawei , Lu, Guodong , Zhou, Jie et al. FgRab5 and FgRab7 are essential for endosomes biogenesis and non-redundantly recruit the retromer complex to the endosomes in Fusarium graminearum . | STRESS BIOLOGY , 2021 , 1 (1) . |
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During the infection and colonization process, the rice blast fungus Magnaporthe oryzae faces various challenges from hostile environment, such as nutrient limitation and carbon stress, while carbon catabolite repression (CCR) mechanism would facilitate the fungus to shrewdly and efficiently utilize carbon nutrients under fickle nutritional conditions since it ensures the preferential utilization of most preferred carbon sources through repressing the expression of enzymes required for the utilization of less preferred carbon sources. Researches on M. oryzae CCR have made some progress, however the involved regulation mechanism is still largely obscured, especially, little is known about the key carbon catabolite repressor CreA. Here we identified and characterized the biological functions of the CreA homolog MoCreA in M. oryzae. MoCreA is constitutively expressed throughout all the life stages of the fungus, and it can shuttle between nucleus and cytoplasm which is induced by glucose. Following functional analyses of MoCreA suggested that it was required for the vegetative growth, conidiation, appressorium formation and pathogenicity of M. oryzae. Moreover, comparative transcriptomic analysis revealed that disruption of MoCreA resulted in the extensive gene expression variations, including a large number of carbon metabolism enzymes, transcription factors and pathogenicity-related genes. Taken together, our results demonstrated that, as a key regulator of CCR, MoCreA plays a vital role in precise regulation of the asexual development and pathogenicity of the rice blast fungus.
Keyword ：
Asexual development Asexual development Carbon catabolite repression Carbon catabolite repression Comparative transcriptomic analysis Comparative transcriptomic analysis Differentially expressed genes Differentially expressed genes Magnaporthe oryzae Magnaporthe oryzae MoCreA MoCreA
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| GB/T 7714 | Hong, Yonghe , Cai, Renli , Guo, Jiayuan et al. Carbon catabolite repressor MoCreA is required for the asexual development and pathogenicity of the rice blast fungus [J]. | FUNGAL GENETICS AND BIOLOGY , 2021 , 146 . |
| MLA | Hong, Yonghe et al. "Carbon catabolite repressor MoCreA is required for the asexual development and pathogenicity of the rice blast fungus" . | FUNGAL GENETICS AND BIOLOGY 146 (2021) . |
| APA | Hong, Yonghe , Cai, Renli , Guo, Jiayuan , Zhong, Zhenhui , Bao, Jiandong , Wang, Zonghua et al. Carbon catabolite repressor MoCreA is required for the asexual development and pathogenicity of the rice blast fungus . | FUNGAL GENETICS AND BIOLOGY , 2021 , 146 . |
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Autophagy is responsible for maintaining fundamental cellular homeostasis and is, therefore, essential for diverse development processes. This study reported that PoElp3, the putative catalytic subunit of Elongator complex, is involved in the maintenance of autophagy homeostasis to facilitate asexual development and pathogenicity in the rice blast fungus Pyricularia oryzae. It was found that the Delta Poelp3 strains were defective in vegetative growth, conidiation, stress response, and pathogenicity. The mutants exhibited hyper-activated autophagy in the vegetative hyphae under both nutrient-rich and nutrient-deficient conditions. The hyper-activation of autophagy possibly suppressed the production of vegetative hyphae in the Delta Poelp3 strains. Moreover, the Delta Poelp3 strains were found to be more sensitive to rapamycin during vegetative-and invasive-hyphal growth but have no effect on Target-of-Rapamycin (TOR) signaling inhibition. Taken together, these results demonstrated that PoElp3 is involved in asexual development and pathogenicity by regulating autophagy in the rice blast fungus.
Keyword ：
asexual development asexual development autophagy autophagy elongator elongator Elp3 Elp3 pathogenicity pathogenicity Pyricularia oryzae Pyricularia oryzae
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| GB/T 7714 | Zhang Li-mei , Chen Shu-ting , Qi Min et al. The putative elongator complex protein Elp3 is involved in asexual development and pathogenicity by regulating autophagy in the rice blast fungus [J]. | JOURNAL OF INTEGRATIVE AGRICULTURE , 2021 , 20 (11) : 2944-2956 . |
| MLA | Zhang Li-mei et al. "The putative elongator complex protein Elp3 is involved in asexual development and pathogenicity by regulating autophagy in the rice blast fungus" . | JOURNAL OF INTEGRATIVE AGRICULTURE 20 . 11 (2021) : 2944-2956 . |
| APA | Zhang Li-mei , Chen Shu-ting , Qi Min , Cao Xue-qi , Liang Nan , Li Qian et al. The putative elongator complex protein Elp3 is involved in asexual development and pathogenicity by regulating autophagy in the rice blast fungus . | JOURNAL OF INTEGRATIVE AGRICULTURE , 2021 , 20 (11) , 2944-2956 . |
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