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学者姓名：胡开辉
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Abstract ：
Lyophyllum decastes is a type of edible and medicinal mushroom with high nutritional value. However, it can be infected by fungi during the fruiting process, which impairs the development of the industry. In this study, one pathogenic fungus was isolated from the diseased fruiting bodies of L. decastes. Morphological and molecular identification clarified that the causal agent of the disease was Cladobotryum mycophilum. Koch's postulates showed that C. mycophilum could infect L. decastes, and seriously affect mushroom yields. The biological properties of C. mycophilum were investigated. The data showed that the most suitable temperature for the growth of C. mycophilum was 25 degrees C with a mean growth rate of 21.45 mm d- 1 on PDA. C. mycophilum was better suited to growing in neutral or slightly acidic environments. The suitable carbon source and nitrogen source for C. mycophilum growth were lactose and peptone or yeast extract respectively. The IC50s (Half maximal inhibitory concentration) values of carbendazim and prochloraz for inhibiting C. mycophilum are respectively 1.244 mu g.mL- 1 and 3.323 mu g.mL- 1. 10 mu g.mL- 1 carbendazim can effectively control C. mycophilum during the L. decastes fruiting process. The results of this study can provide knowledge for the control of cobweb disease during the L. decastes fruiting process.
Keyword ：
Cladobotryum mycophilum Cladobotryum mycophilum Cobweb disease control Cobweb disease control Fruiting process Fruiting process Identification Identification Lyophyllum decastes Lyophyllum decastes
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| GB/T 7714 | Wu, Taorui , Chen, Youlong , Zhang, Wenxin et al. Identification, biological characterization of Cladobotryum mycophilum and its control during the fruiting process of Lyophyllum decastes [J]. | ARCHIVES OF MICROBIOLOGY , 2025 , 207 (2) . |
| MLA | Wu, Taorui et al. "Identification, biological characterization of Cladobotryum mycophilum and its control during the fruiting process of Lyophyllum decastes" . | ARCHIVES OF MICROBIOLOGY 207 . 2 (2025) . |
| APA | Wu, Taorui , Chen, Youlong , Zhang, Wenxin , Pu, Zijian , Li, Jianhua , Xu, Yawen et al. Identification, biological characterization of Cladobotryum mycophilum and its control during the fruiting process of Lyophyllum decastes . | ARCHIVES OF MICROBIOLOGY , 2025 , 207 (2) . |
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The homologous recombination strategy has a long history of editing Saccharomyces cerevisiae target genes. The application of CRISPR/Cas9 strategy to editing target genes in S. cerevisiae has also received a lot of attention in recent years. All findings seem to indicate that editing relevant target genes in S. cerevisiae is an extremely easy event. In this study, we systematically analyzed the advantages and disadvantages of homologous recombination (HR) strategy, CRISPR/Cas9 strategy, and CRISPR/Cas9 combined homology-mediated repair (CRISPR/Case9HDR) strategy in knocking out BY4742 ade2. Our data showed that when the ade2 was knocked out by HR strategy, a large number of clones appeared to be off-target, and 10 %-80 % of the so-called knockout clones obtained were heteroclones. When the CRISPR/Cas9 strategy was applied, 60% of clones were off-target and the rest were all heteroclones. Interestingly, most of the cells were edited successfully, but at least 60 % of the clones were heteroclones, when the CRISPR/Cas9-HDR strategy was employed. Our results clearly showed that the emergence of heteroclone seems inevitable regardless of the strategies used for editing BY4742 ade2. Given the characteristics of BY4742 defective in ade2 showing red on the YPD plate, we attempted to build an efficient yeast gene editing strategy, in which the CRISPR/Cas9 combines homology-mediated repair template carrying an ade2 expression cassette, BY4742(ade2 Delta 0) as the start strain. We used this strategy to successfully achieve 100 % knockout efficiency of trp1, indicating that technical challenges of how to easily screen out pure knockout clones without color phenotype have been solved. Our data showed in this study not only establishes an efficient yeast gene knockout strategy with dual auxotrophy coupled red labeling but also provides new ideas and references for the knockout of target genes in the monokaryotic mycelium of macrofungi.
Keyword ：
ade2 ade2 CRISPR/Cas9 CRISPR/Cas9 Homologous recombination Homologous recombination Homology-mediated repair Homology-mediated repair Saccharomyces cerevisiae Saccharomyces cerevisiae
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| GB/T 7714 | Li, Jianhua , Wu, Taorui , Wang, Jialong et al. Dual auxotrophy coupled red labeling strategy for efficient genome editing in Saccharomyces cerevisiae [J]. | FUNGAL GENETICS AND BIOLOGY , 2024 , 173 . |
| MLA | Li, Jianhua et al. "Dual auxotrophy coupled red labeling strategy for efficient genome editing in Saccharomyces cerevisiae" . | FUNGAL GENETICS AND BIOLOGY 173 (2024) . |
| APA | Li, Jianhua , Wu, Taorui , Wang, Jialong , Chen, Youlong , Zhang, Wenxin , Cai, Lijun et al. Dual auxotrophy coupled red labeling strategy for efficient genome editing in Saccharomyces cerevisiae . | FUNGAL GENETICS AND BIOLOGY , 2024 , 173 . |
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本实用新型公开了一种食用菌培育霉变喷药装置，主罐体，所述主罐体下方设置有喷药头；转动安装在所述主罐体侧下方的若干个活动杆；所述活动杆包括第一弯折部、第二弯折部、以及一端连接所述第一弯折部下端的连接部，所述连接部另一端与所述第二弯折部固定连接，通过喷药头对正下方的霉变培育袋进行喷药处理。为预防喷药过程中，霉菌孢子受到冲击造成飞散外溢的情况，设置了套膜将主罐体以下部分包覆，形成只有下方开口半封闭的喷药区，而为进一步加强套膜与活动杆的固定效果，故在所述第二弯折部与连接部的侧面设有若干个通孔，所述通孔与套膜内侧缝接固定。
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| GB/T 7714 | 金文松 , 胡开辉 , 孙淑静 et al. 一种食用菌培育霉变喷药装置 : CN202321892039.X[P]. | 2023-07-18 . |
| MLA | 金文松 et al. "一种食用菌培育霉变喷药装置" : CN202321892039.X. | 2023-07-18 . |
| APA | 金文松 , 胡开辉 , 孙淑静 , 李佳欢 . 一种食用菌培育霉变喷药装置 : CN202321892039.X. | 2023-07-18 . |
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本发明公开了一种海鲜菇酱油制备方法，其步骤包括：S1，选取新鲜海鲜菇为原料，清洗后，按料液比1：4比例加水，置于负压提取罐中在温度区间温度70‑80℃内进行提取，且过滤掉海鲜菇的残留碎屑及杂质，获得海鲜菇提取液；S2，将海鲜菇提取液按照水体体积20‑30％，置于温度区间为50～55℃水中稀释，本发明以海鲜菇粉替代大豆致敏物豆粕，解决了豆制品过敏人群食用酱油会引起过敏反应等问题；通过梯度变温法烘干海鲜菇，同时通过烘干提升海鲜菇风味。结合采用先高温后中温的变温发酵工艺，显著改善了低盐固态酱油产品的风味，提高了其香味、营养价值，既方便了对豆类产品过敏人群的食用，也对其他无过敏人群提供了一种可供选择的风味酱油。
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| GB/T 7714 | 胡开辉 , 李佳欢 , 金文松 et al. 一种海鲜菇酱油制备方法 : CN202311841688.1[P]. | 2023-12-28 . |
| MLA | 胡开辉 et al. "一种海鲜菇酱油制备方法" : CN202311841688.1. | 2023-12-28 . |
| APA | 胡开辉 , 李佳欢 , 金文松 , 孙淑静 , 陈利丁 . 一种海鲜菇酱油制备方法 : CN202311841688.1. | 2023-12-28 . |
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通过观察8株黑皮鸡枞菌株固体、液体发酵过程中菌丝生长情况，比较其发酵液营养成分，以ABTS自由基清除率、DPPH自由基清除率、羟自由基清除率等抗氧化能力指标对菌株进行筛选。结果：菌株HP1的菌丝活力强，菌球干重大，为0.827 1 g·(100 mL)~(-1)；发酵液还原糖含量为50.29 mg·mL~(-1)，可溶性蛋白含量为38.47μg·mL~(-1)；抗氧化物质含量高，总酚、总黄酮含量为240.12、23.23μg·mL~(-1)；抗氧化能力最强，其稀释液对ABTS自由基、DPPH自由基、羟自由基的清除率为54.28%、78.08%、66.06%，对铁离子的还原能力最强，吸光度为0.598。结果表明，黑皮鸡枞HP1菌株长势优良、菌丝浓密，富含黄酮、总酚等活性物质，抗氧化能力强，可用于后续黑皮鸡枞抗氧化产品的开发利用。
Keyword ：
抗氧化活性 抗氧化活性 菌株筛选 菌株筛选 菌种培养 菌种培养 黑皮鸡枞 黑皮鸡枞
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| GB/T 7714 | 李春兰 , 李佳欢 , 胡开辉 et al. 高抗氧化活性黑皮鸡枞优良菌株筛选 [J]. | 南方农业 , 2023 , 17 (15) : 29-33 . |
| MLA | 李春兰 et al. "高抗氧化活性黑皮鸡枞优良菌株筛选" . | 南方农业 17 . 15 (2023) : 29-33 . |
| APA | 李春兰 , 李佳欢 , 胡开辉 , 梁鹏 . 高抗氧化活性黑皮鸡枞优良菌株筛选 . | 南方农业 , 2023 , 17 (15) , 29-33 . |
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Hypsizygus marmoreus is one of the main industrially cultivated varieties of edible fungi, with a delicious taste and high nutritional value. However, the long harvest period of 130-150 days greatly limits its large-scale expansion. This study aimed to investigate the effects of central carbon metabolism (CCM) on the mycelial growth performance and fruiting body formation of H. marmoreus. Nine edible fungi with different harvest periods were collected and used to evaluate their intracellular carbon metabolic differences in the CCM, which revealed that the imbalanced distribution of intracellular carbon metabolic levels in the CCM of H. marmoreus might be one of the key factors resulting in a slow mycelial growth rate and a long harvest period. Further analysis by three strategies, including metabolomics, adaptation of different carbon sources, and chemical interference, confirmed that low carbon flux into the pentose phosphate pathway (PPP) limited the supply of raw materials, reduced power, and thus influenced the mycelial growth of H. marmoreus. Furthermore, four transformants with increased expression levels of glucose-6-phosphate dehydrogenase (G6PDH), a key rate-limiting enzyme in the PPP of H. marmoreus, were developed and showed more extracellular soluble protein secretion and higher sugar assimilation rates, as well as improved mycelial growth rates in bottle substrate mixtures. Finally, cultivation experiments indicated that the maturation periods of the fruiting body with similar to 4-5 days in advance and the maximum fruiting body yield of 574.8 g per bag with an increase of 7.4% were achieved by improving the G6PDH expression level of the PPP in H. marmoreus. This study showed that CCM played an important role in the mycelial growth and development of H. marmoreus, which provided new insights for future advancements in cultivating and breeding edible fungi.
Keyword ：
central carbon metabolism (CCM) central carbon metabolism (CCM) glucose-6-phosphate dehydrogenase (G6PDH) glucose-6-phosphate dehydrogenase (G6PDH) harvest period harvest period Hypsizygus marmoreus Hypsizygus marmoreus pentose phosphate pathway (PPP) pentose phosphate pathway (PPP)
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| GB/T 7714 | Lin, Hui , Li, Pengfei , Ma, Lu et al. Analysis and modification of central carbon metabolism in Hypsizygus marmoreus for improving mycelial growth performance and fruiting body yield [J]. | FRONTIERS IN MICROBIOLOGY , 2023 , 14 . |
| MLA | Lin, Hui et al. "Analysis and modification of central carbon metabolism in Hypsizygus marmoreus for improving mycelial growth performance and fruiting body yield" . | FRONTIERS IN MICROBIOLOGY 14 (2023) . |
| APA | Lin, Hui , Li, Pengfei , Ma, Lu , Lai, Shufang , Sun, Shujing , Hu, Kaihui et al. Analysis and modification of central carbon metabolism in Hypsizygus marmoreus for improving mycelial growth performance and fruiting body yield . | FRONTIERS IN MICROBIOLOGY , 2023 , 14 . |
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本发明公开了一种鹿茸菇液体菌种培养基及采用该培养基栽培鹿茸菇的方法，鹿茸菇液体菌种培养基，包括以下重量份的组分：全麦粉40‑60份、花生饼粉18‑26份、K2HPO41.5‑2.5份、MgSO4·7H2O 1.5‑2.5份。采用上述鹿茸菇液体菌种培养基栽培鹿茸菇的方法，包括以下步骤：制备鹿茸菇一级种子液，然后采用上述鹿茸菇液体菌种培养基对一级种子液进行发酵培养，得到发酵液，将发酵液接种到栽培料中进行出菇培养。本发明提供的鹿茸菇液体菌种培养基中全麦粉可为鹿茸菇提供碳源，花生饼粉为鹿茸菇提供氮源，K2HPO4和MgSO4·7H2O为鹿茸菇提供无机盐，培养基成本低，可有效提高鹿茸菇的菌丝生物量。
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| GB/T 7714 | 金文松 , 胡开辉 , 孙淑静 et al. 一种鹿茸菇液体菌种培养基及采用该培养基栽培鹿茸菇的方法 : CN202211224385.0[P]. | 2022-10-08 . |
| MLA | 金文松 et al. "一种鹿茸菇液体菌种培养基及采用该培养基栽培鹿茸菇的方法" : CN202211224385.0. | 2022-10-08 . |
| APA | 金文松 , 胡开辉 , 孙淑静 , 李佳欢 , 单灿灿 . 一种鹿茸菇液体菌种培养基及采用该培养基栽培鹿茸菇的方法 : CN202211224385.0. | 2022-10-08 . |
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本发明公开了一种秀珍菇吊袋划线高效出菇栽培方法，所述的秀珍菇吊袋划线高效出菇栽培方法包括以下步骤：①母种制备；②一级种子制备；③栽培种制备；④栽培料制备；⑤接种；⑥养菌；⑦吊绳支架制作；⑧菌包上架；⑨开袋模式；⑩出菇管理；采菇；转潮期处理。本发明的优点在于：提供一种新型秀珍菇高效出菇栽培方法，该方法可以显著提高秀珍菇单潮菇产量，以达到缩短秀珍菇生产周期目的，提高生产效率，减轻秀珍菇生产中的病虫害问题，且减少病虫害造成的损失，实现秀珍菇产业出菇菇房高效周转利用，释放劳动力，降低生产成本。
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| GB/T 7714 | 金文松 , 胡开辉 , 孙淑静 et al. 一种秀珍菇吊袋划线高效出菇栽培方法 : CN202210063966.4[P]. | 2022-01-20 . |
| MLA | 金文松 et al. "一种秀珍菇吊袋划线高效出菇栽培方法" : CN202210063966.4. | 2022-01-20 . |
| APA | 金文松 , 胡开辉 , 孙淑静 , 李佳欢 , 夏俊慧 . 一种秀珍菇吊袋划线高效出菇栽培方法 : CN202210063966.4. | 2022-01-20 . |
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In this study, fresh Lyophyllum decastes was dried using hot air drying (HAD), hot air combined with vacuum drying (HAVD), and vacuum freeze drying (VFD). Additionally, the quality and volatile compounds were analyzed. VFD achieved the best color retention, the highest rehydration capacity, and the slightest damaged tissue structure; however, it recorded the longest drying time and the highest energy consumption. HAD was the most energy-efficient of the three methods. Furthermore, the products with more hardness and elasticity were obtained by HAD and HAVD-this finding was convenient for transportation. In addition, GC-IMS demonstrated that the flavor components had significantly changed after drying. A total of 57 volatile flavor compounds was identified, and the aldehyde, alcohol, and ketone compounds were the primary ingredient of the L. decastes flavor component, whereby the relative content of the HAD sample was apparently higher than HAVD and VFD. Taken together, VFD was better at preserving the color and shape of fresh L. decastes, but HAD was more appropriate for drying L. decastes because of the lower energy consumption, and was more economical. Meanwhile, HAD could be used to produce a more intense aroma.
Keyword ：
drying methods drying methods gas chromatography-ion mobility spectrometry (GC-IMS) gas chromatography-ion mobility spectrometry (GC-IMS) Lyophyllum decastes Lyophyllum decastes physicochemical aspects physicochemical aspects principal component analysis (PCA) principal component analysis (PCA) volatile compounds volatile compounds
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| GB/T 7714 | Yang, Bin , Huang, Jianhang , Jin, Wensong et al. Effects of Drying Methods on the Physicochemical Aspects and Volatile Compounds of Lyophyllum decastes [J]. | FOODS , 2022 , 11 (20) . |
| MLA | Yang, Bin et al. "Effects of Drying Methods on the Physicochemical Aspects and Volatile Compounds of Lyophyllum decastes" . | FOODS 11 . 20 (2022) . |
| APA | Yang, Bin , Huang, Jianhang , Jin, Wensong , Sun, Shujing , Hu, Kaihui , Li, Jiahuan . Effects of Drying Methods on the Physicochemical Aspects and Volatile Compounds of Lyophyllum decastes . | FOODS , 2022 , 11 (20) . |
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Depolysaccharide residues of edible fungus Pleurotus eryngii (dePSR-Pe), a mushroom industry waste, have abundant cellulose. In this study, the cellulose nanocrystals of P. eryngii (PeCNs) were extracted by hydrochloric acid. Results showed that the length of PeCNs is 469 +/- 76.41 nm with a high aspect ratio of 40-100 and needle morphology. The structural characterization revealed that PeCNs had good thermal stability (approach 300 degrees C) and high crystallinity (84.2 %). An O/W Pickering emulsion stabilized with PeCNs was prepared to inhibit lipid oxidation and improve the loading capacity of triterpenes of P. coco. Unimodal size distribution of emulsion droplets was obtained under an optimized aqueous-phase condition to form a metastable emulsion, regardless of varying oil-water volume ratio <50/50. In vitro digestion study suggested that triterpenes-loaded Pickering emulsion had 1-3 times higher drug stability than bulk oil. These metastable Pickering emulsions call for fewer nanoparticles and provide a new strategy for the industry application of cellulose nanocrystals at less cost.
Keyword ：
Cellulose nanocrystals Cellulose nanocrystals de-polysaccharide residues de-polysaccharide residues In vitro digestion In vitro digestion Pickering emulsion Pickering emulsion Pleurotus eryngii Pleurotus eryngii Triterpenes Triterpenes
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| GB/T 7714 | Chen, Liding , Lin, Chenghui , Ye, Qing et al. A fungal cellulose nanocrystals-based approach to improve the stability of triterpenes loaded Pickering emulsion [J]. | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES , 2022 , 222 : 438-447 . |
| MLA | Chen, Liding et al. "A fungal cellulose nanocrystals-based approach to improve the stability of triterpenes loaded Pickering emulsion" . | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 222 (2022) : 438-447 . |
| APA | Chen, Liding , Lin, Chenghui , Ye, Qing , Chen, Jianqiu , Chen, Zhihan , Jiang, Jiahui et al. A fungal cellulose nanocrystals-based approach to improve the stability of triterpenes loaded Pickering emulsion . | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES , 2022 , 222 , 438-447 . |
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