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学者姓名：艾育芳
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Abstract ：
It is urgent to mine novel blast-resistant genes in rice and develop new rice varieties with pyramiding blast-resistant genes. In this study, a new blast-resistant gene, OsBRW1, was screened from a set of rice near-isogenic lines (NILs) with different blast-resistant ability. Under the infection of Magnaporthe oryzae (M. oryzae), OsBRW1 in the resistant NIL Pi-4b was highly induced than that in the susceptible NIL Pi-1 and their parent line CO39, and the blast-resistant ability of OsBRW1 was further confirmed by using CRISPR/Cas9 knockout and over-expression methods. The protein encoded by OsBRW1 was a typical NBS-LRR with NB-ARC domain and localized in both cytoplasm and nucleus, and the transient expression of OsBRW1 was capable of triggering hypersensitive response in tobacco leaves. Protein interaction experiments showed that OsBRW1 protein directly interacted with OsSRFP1. At the early infection stage of M. oryzae, OsBRW1 gene induced OsSRFP1 to highly expression level and accumulated H2O2, up-regulated the defence responsive signalling transduction genes and the pathogenesis-related genes and increased JA and SA content in the resistant NIL Pi-4b. By contrary, lower content of endogenous JA and SA in osbrw1 mutants was found at the same stage. After that, OsSRFP1 was down-regulated to constitution abundance to balance the growth of the resistant NIL Pi-4b. In summary, OsBRW1 solicited OsSRFP1 to resist the infection of blast fungus in rice by inducing the synergism of induced systemic resistance (ISR) and system acquired resistance (SAR) and to balance the growth of rice plants.
Keyword ：
growth growth immunity immunity novel blast-resistant gene novel blast-resistant gene rice (Oryza sativa L.) rice (Oryza sativa L.)
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| GB/T 7714 | Ma, Shiwei , Xu, Shichang , Tao, Huan et al. OsBRW1, a novel blast-resistant gene, coded a NBS-LRR protein to interact with OsSRFP1 to balance rice growth and resistance [J]. | PLANT BIOTECHNOLOGY JOURNAL , 2025 , 23 (1) : 250-267 . |
| MLA | Ma, Shiwei et al. "OsBRW1, a novel blast-resistant gene, coded a NBS-LRR protein to interact with OsSRFP1 to balance rice growth and resistance" . | PLANT BIOTECHNOLOGY JOURNAL 23 . 1 (2025) : 250-267 . |
| APA | Ma, Shiwei , Xu, Shichang , Tao, Huan , Huang, Yunxia , Feng, Changqing , Huang, Guanpeng et al. OsBRW1, a novel blast-resistant gene, coded a NBS-LRR protein to interact with OsSRFP1 to balance rice growth and resistance . | PLANT BIOTECHNOLOGY JOURNAL , 2025 , 23 (1) , 250-267 . |
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| GB/T 7714 | Feng, Changqing , Zhu, Xiangrong , Wu, Mingji et al. A Novel Long Non-Coding RNA, OslncBR3, Positively Regulates Rice Resistance to Blast Fungus via Upregulating NLR Genes OsLTG1 and OsLTG2 [J]. | PLANT CELL AND ENVIRONMENT , 2025 , 48 (10) : 7750-7753 . |
| MLA | Feng, Changqing et al. "A Novel Long Non-Coding RNA, OslncBR3, Positively Regulates Rice Resistance to Blast Fungus via Upregulating NLR Genes OsLTG1 and OsLTG2" . | PLANT CELL AND ENVIRONMENT 48 . 10 (2025) : 7750-7753 . |
| APA | Feng, Changqing , Zhu, Xiangrong , Wu, Mingji , Wu, Shuhong , Guo, Jiayi , Xu, Kangze et al. A Novel Long Non-Coding RNA, OslncBR3, Positively Regulates Rice Resistance to Blast Fungus via Upregulating NLR Genes OsLTG1 and OsLTG2 . | PLANT CELL AND ENVIRONMENT , 2025 , 48 (10) , 7750-7753 . |
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Rice blast disease, caused by Magnaporthe oryzae (M. oryzae), is a significant threat to global rice production. Conventional methods for disease management face limitations, emphasizing the importance of sustainable alternatives. In this study, two rice cultivars with different blast resistant abilities, the susceptible variety CO39 and the resistant variety Pi4b, were used as materials to study the effects of Piriformospora indica (Pi) on the resistance to M. oryzae infection and rice growth. The in vitro tests revealed no direct antagonistic interaction between Pi and M. oryzae. However, the in vivo experiments showed that Pi promoted plant growth by increasing root and shoot length, chlorophyll content, and nitrogen uptake, particularly in CO39 during pathogen infection. Pi inoculation also significantly reduced disease severity, which was indicated by smaller lesion areas and shorter lesion lengths in both cultivars but a more pronounced effect in CO39. This occurred due to the decreasing levels of MDA and the modulating activity of antioxidant enzymes in Pi-inoculated rice plants. At the early stage of M. oryzae infection, the expression of the ethylene signaling gene OsEIN2 and the gibberellin biosynthesis gene OsGA20ox1 in Pi-inoculated CO39 decreased but significantly increased in both rice cultivars at the later stage. The reverse was found for the pathogenesis-related (PR) genes OsPR10 and OsPBZ1 and the blast-resistant genes OsBRG1, OsBRG2, and OsBRW1, suggesting early growth suppression for rice resilience to blast followed by a later shift back to growth. Meanwhile, Pi inoculation increased OsCesA9 expression in rice to strengthen cell walls and establish the primary defense barrier against M. oryzae and upregulated the expression of OsNPR1 without a significant difference in CO39 but downregulated it in Pi4b to activate PR genes to enhance plant resistance. In summary, these results underscore the potential of Pi as a sustainable biological control agent for rice blast disease, which is particularly beneficial for blast-susceptible rice cultivars.
Keyword ：
balance between growth and defense balance between growth and defense Magnaporthe oryzae Magnaporthe oryzae Piriformospora indica Piriformospora indica rice (Oryza sativa L.) rice (Oryza sativa L.)
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| GB/T 7714 | Kabore, Manegdebwaoga Arthur Fabrice , Huang, Guanpeng , Feng, Changqing et al. Piriformospora indica Enhances Rice Blast Resistance and Plant Growth [J]. | AGRONOMY-BASEL , 2025 , 15 (1) . |
| MLA | Kabore, Manegdebwaoga Arthur Fabrice et al. "Piriformospora indica Enhances Rice Blast Resistance and Plant Growth" . | AGRONOMY-BASEL 15 . 1 (2025) . |
| APA | Kabore, Manegdebwaoga Arthur Fabrice , Huang, Guanpeng , Feng, Changqing , Wu, Shuhong , Guo, Jiayi , Wu, Guofeng et al. Piriformospora indica Enhances Rice Blast Resistance and Plant Growth . | AGRONOMY-BASEL , 2025 , 15 (1) . |
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Grain size is one of the critical factors determining rice yield. Previous studies have found the grain-size-regulating function of G alpha in rice. However, the regulatory mechanism underlying the development of rice grain mediated by G alpha is still unclear. To reveal the functional mechanism of G alpha in grain size regulation, a mutant of G alpha (G alpha-Cas9) was firstly constructed through a CRISPR/Cas9 strategy and was then grown in a greenhouse and field. The results showed that the seed length, plant height, 1000-grain weight, and spike length were significantly decreased in G alpha-Cas9 compared to wild-type (WT) Pi-4b. During the grain filling stage, the increase in the grain dry weight of Pi-4b occurred earlier than that of G alpha-Cas9. The total starch content and amylose content of matured grains of Pi-4b were higher than those of G alpha-Cas9. Secondly, transcriptome sequencing analysis of G alpha-Cas9 and Pi-4b during grain filling was performed to elucidate the functional pathways regulated by G alpha. In total, 2867 and 4534 differentially expressed genes (DEGs) were discovered at 5 DAF and 10 DAF, and the starch and sucrose metabolism pathway enriched by DEGs was involved in grain size regulation mediated by G alpha. G alpha regulated the expression of starch-synthesis-related genes during grain filling, and the G alpha protein interacted with OsNYC4 to trigger the sugar signaling pathway to promote starch accumulation in grain. Additionally, the GW2-WG1-OsbZIP47 pathway was switched off by G alpha to relieve the inhibition of rice grain development. In this study, the results should provide new insights into the G protein signal transduction pathway.
Keyword ：
G alpha of heterotrimeric G protein G alpha of heterotrimeric G protein grain filling grain filling grain size grain size starch synthesis starch synthesis
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| GB/T 7714 | Ma, Shiwei , Sun, Yiqiong , Chen, Xuan et al. Gα Solicits OsNYC4 and GW2-WG1-OsbZIP47 Modules to Regulate Grain Size in Rice (Oryza sativa L.) [J]. | AGRONOMY-BASEL , 2024 , 14 (7) . |
| MLA | Ma, Shiwei et al. "Gα Solicits OsNYC4 and GW2-WG1-OsbZIP47 Modules to Regulate Grain Size in Rice (Oryza sativa L.)" . | AGRONOMY-BASEL 14 . 7 (2024) . |
| APA | Ma, Shiwei , Sun, Yiqiong , Chen, Xuan , Guo, Jiayi , Wu, Shuhong , Wu, Guofeng et al. Gα Solicits OsNYC4 and GW2-WG1-OsbZIP47 Modules to Regulate Grain Size in Rice (Oryza sativa L.) . | AGRONOMY-BASEL , 2024 , 14 (7) . |
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Transcription factors (TFs) play critical roles in mediating the plant response to various abiotic stresses, particularly heat stress. Plants respond to elevated temperatures by modulating the expression of genes involved in diverse metabolic pathways, a regulatory process primarily governed by multiple TFs in a networked configuration. Many TFs, such as WRKY, MYB, NAC, bZIP, zinc finger protein, AP2/ERF, DREB, ERF, bHLH, and brassinosteroids, are associated with heat shock factor (Hsf) families, and are involved in heat stress tolerance. These TFs hold the potential to control multiple genes, which makes them ideal targets for enhancing the heat stress tolerance of crop plants. Despite their immense importance, only a small number of heat-stress-responsive TFs have been identified in rice. The molecular mechanisms underpinning the role of TFs in rice adaptation to heat stress still need to be researched. This study identified three TF genes, including OsbZIP14, OsMYB2, and OsHSF7, by integrating transcriptomic and epigenetic sequencing data analysis of rice in response to heat stress. Through comprehensive bioinformatics analysis, we demonstrated that OsbZIP14, one of the key heat-responsive TF genes, contained a basic-leucine zipper domain and primarily functioned as a nuclear TF with transcriptional activation capability. By knocking out the OsbZIP14 gene in the rice cultivar Zhonghua 11, we observed that the knockout mutant OsbZIP14 exhibited dwarfism with reduced tiller during the grain-filling stage. Under high-temperature treatment, it was also demonstrated that in the OsbZIP14 mutant, the expression of the OsbZIP58 gene, a key regulator of rice seed storage protein (SSP) accumulation, was upregulated. Furthermore, bimolecular fluorescence complementation (BiFC) experiments uncovered a direct interaction between OsbZIP14 and OsbZIP58. Our results suggested that OsbZIP14 acts as a key TF gene through the concerted action of OsbZIP58 and OsbZIP14 during rice filling under heat stress. These findings provide good candidate genes for genetic improvement of rice but also offer valuable scientific insights into the mechanism of heat tolerance stress in rice.
Keyword ：
heat stress heat stress Oryza sativa L Oryza sativa L OsbZIP14 OsbZIP14 transcription factors transcription factors
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| GB/T 7714 | Qiu, Fuxiang , Zheng, Yingjie , Lin, Yao et al. Integrated ATAC-Seq and RNA-Seq Data Analysis to Reveal OsbZIP14 Function in Rice in Response to Heat Stress [J]. | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES , 2023 , 24 (6) . |
| MLA | Qiu, Fuxiang et al. "Integrated ATAC-Seq and RNA-Seq Data Analysis to Reveal OsbZIP14 Function in Rice in Response to Heat Stress" . | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 24 . 6 (2023) . |
| APA | Qiu, Fuxiang , Zheng, Yingjie , Lin, Yao , Woldegiorgis, Samuel Tareke , Xu, Shichang , Feng, Changqing et al. Integrated ATAC-Seq and RNA-Seq Data Analysis to Reveal OsbZIP14 Function in Rice in Response to Heat Stress . | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES , 2023 , 24 (6) . |
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Due to global warming, high temperature is a significant environmental stress for rice production. Rice (Oryza sativa L.), one of the most crucial cereal crops, is also seriously devastated by Magnaporthe oryzae. Therefore, it is essential to breed new rice cultivars with blast and heat tolerance. Although progress had been made in QTL mapping and RNA-seq analysis in rice in response to blast and heat stresses, there are few reports on simultaneously mining blast-resistant and heat-tolerant genes. In this study, we separately conducted meta-analysis of 839 blast-resistant and 308 heat-tolerant QTLs in rice. Consequently, 7054 genes were identified in 67 blast-resistant meta-QTLs with an average interval of 1.00 Mb. Likewise, 6425 genes were obtained in 40 heat-tolerant meta-QTLs with an average interval of 1.49 Mb. Additionally, using differentially expressed genes (DEGs) in the previous research and GO enrichment analysis, 55 DEGs were co-located on the common regions of 16 blast-resistant and 14 heat-tolerant meta-QTLs. Among, OsChib3H-c, OsJAMyb, Pi-k, OsWAK1, OsMT2b, OsTPS3, OsHI-LOX, OsACLA-2 and OsGS2 were the significant candidate genes to be further investigated. These results could provide the gene resources for rice breeding with excellent resistance to these 2 stresses, and help to understand how plants response to the combination stresses of blast fungus and high temperature.
Keyword ：
) ) candidate genes candidate genes high temperature high temperature Magnaporthe oryzae Magnaporthe oryzae meta-analysis meta-analysis QTL QTL rice (Oryza sativa L rice (Oryza sativa L RNA-seq RNA-seq
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| GB/T 7714 | Tian, Tian , Chen, Lijuan , Ai, Yufang et al. Selection of Candidate Genes Conferring Blast Resistance and Heat Tolerance in Rice through Integration of Meta-QTLs and RNA-Seq [J]. | GENES , 2022 , 13 (2) . |
| MLA | Tian, Tian et al. "Selection of Candidate Genes Conferring Blast Resistance and Heat Tolerance in Rice through Integration of Meta-QTLs and RNA-Seq" . | GENES 13 . 2 (2022) . |
| APA | Tian, Tian , Chen, Lijuan , Ai, Yufang , He, Huaqin . Selection of Candidate Genes Conferring Blast Resistance and Heat Tolerance in Rice through Integration of Meta-QTLs and RNA-Seq . | GENES , 2022 , 13 (2) . |
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【目的】查尔酮合成酶是黄酮类生物合成途径中的第1个限速酶基因。从辣木中克隆查尔酮合成酶基因MoCHS1,并对其进行生物信息学分析,为进一步研究其生物学功能提供基础数据。【方法】根据NCBI数据库中的辣木基因组信息设计引物,以辣木叶片cDNA和基因组DNA为模板,PCR扩增获得MoCHS1基因序列。利用生物信息学方法分析其序列特征,使用DNAMAN9.0和MEGA10.0软件进行多重比对和构建系统进化树。【结果】MoCHS1ORF序列长度为1 185 bp,编码394个氨基酸,基因组序列长1 387 bp,含有2个外显子和1个内含子。生物信息学分析表明,MoCHS1为稳定的亲水蛋白,以α-螺旋(45.43%)和不规则卷曲(31.98%)为主。MoCHS1含有查尔酮合成酶家族的保守序列和酶活性位点的关键氨基酸残基,包括7个环化袋氨基酸残基、3个辅酶A活性结合位点、半胱氨酸(Cys)-组氨酸(His)-天冬氨酸(Asn)三联体催化位点和查尔酮合成酶基因家族的2个高度保守的特征序列(RLMMYQQGCFAGGTVLR和GVLFGFGPGL),与其他物种的CHS序列一致性较高。系统进化分析显示,MoCHS1与番木瓜聚在一类,说明其亲缘关系最近。【结论】成功分离了一个辣木查尔酮合成酶基因MoCHS1基因序列,该基因推测编码的氨基酸序列具有CHS家族蛋白的典型保守结构特征。研究结果有助于进一步研究辣木查尔酮合成酶基因的调控及基因家族进化机制和类黄酮合成调控机理。
Keyword ：
查尔酮合成酮 查尔酮合成酮 生物信息学分析 生物信息学分析 类黄酮 类黄酮 辣木 辣木
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| GB/T 7714 | 林玲 , 方健超 , 陈观水 et al. 辣木查尔酮合成酶(CHS)基因的克隆及其序列分析 [J]. | 福建农业学报 , 2021 , 36 (05) : 549-555 . |
| MLA | 林玲 et al. "辣木查尔酮合成酶(CHS)基因的克隆及其序列分析" . | 福建农业学报 36 . 05 (2021) : 549-555 . |
| APA | 林玲 , 方健超 , 陈观水 , 艾育芳 . 辣木查尔酮合成酶(CHS)基因的克隆及其序列分析 . | 福建农业学报 , 2021 , 36 (05) , 549-555 . |
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生物化学是生命科学领域一门重要的专业基础课,但因其知识点多、理论性强、且知识点与相关技术更新较快,如何打破传统教学的框架,开展有效的教学改革具有较大的现实意义。本文探讨了科研实践在教学改革中的作用,并深入分析了科研实践如何与教学相互促进,有助于提高生物化学的教学效率。
Keyword ：
教学改革 教学改革 生物化学 生物化学 科研实践 科研实践
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| GB/T 7714 | 张灵玲 , 牟少亮 , 艾育芳 et al. 科研实践与生物化学教学相长的体会 [J]. | 生命的化学 , 2020 , 40 (12) : 2303-2306 . |
| MLA | 张灵玲 et al. "科研实践与生物化学教学相长的体会" . | 生命的化学 40 . 12 (2020) : 2303-2306 . |
| APA | 张灵玲 , 牟少亮 , 艾育芳 , 马洪丽 . 科研实践与生物化学教学相长的体会 . | 生命的化学 , 2020 , 40 (12) , 2303-2306 . |
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Rice blast, caused by the fungus, Magnaporthe grisea (M. grisea), lead to the decrease of rice yields widely and destructively, threatening global food security. Although many resistant genes had been isolated and identified in various rice varieties, it is still not enough to clearly understand the mechanism of race-specific resistant ability in rice, especially on the protein level. In this research, proteomic methods were employed to analyze the differentially expressed proteins (DEPs) in susceptible rice variety CO39 and its two near isogenic lines (NILs), CN-4a and CN-4b, in response to the infection of two isolates with different pathogenicity, GUY11 and 81278ZB15. A total of 50 DEPs with more than 1.5-fold reproducible change were identified. At 24 and 48 hpi of GUY11, 32 and 16 proteins in CN-4b were up-regulated, among which 16 and five were paralleled with the expression of their corresponding RNAs. Moreover, 13 of 50 DEPs were reported to be induced by M. grisea in previous publications. Considering the phenotypes of the three tested rice varieties, we found that 21 and 23 up-regulated proteins were responsible for the rice resistant ability to the two different blast isolates, 81278ZB15 and GUY11, respectively. Two distinct branches corresponding to GUY11 and 81278ZB15 were observed in the expression and function of the module cluster of DEPs, illuminating that the DEPs could be responsible for race-specific resistant ability in rice. In other words, DEPs in rice are involved in different patterns and functional modules' response to different pathogenic race infection, inducing race-specific resistant ability in rice.
Keyword ：
blast fungus (M. grisea) blast fungus (M. grisea) different pathogenic isolates different pathogenic isolates race-specific resistance race-specific resistance rice (Oryza sativa L.) rice (Oryza sativa L.)
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| GB/T 7714 | Ma, Shiwei , Lin, Shoukai , Wang, Menglin et al. Differential Expression Proteins Contribute to Race-Specific Resistant Ability in Rice (Oryza sativa L.) [J]. | PLANTS-BASEL , 2019 , 8 (2) . |
| MLA | Ma, Shiwei et al. "Differential Expression Proteins Contribute to Race-Specific Resistant Ability in Rice (Oryza sativa L.)" . | PLANTS-BASEL 8 . 2 (2019) . |
| APA | Ma, Shiwei , Lin, Shoukai , Wang, Menglin , Zou, Yang , Tao, Huan , Liu, Wei et al. Differential Expression Proteins Contribute to Race-Specific Resistant Ability in Rice (Oryza sativa L.) . | PLANTS-BASEL , 2019 , 8 (2) . |
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Background Rice (Oryza sativa L.) yield is limited inherently by environmental stresses, including biotic and abiotic stresses. Thus, it is of great importance to perform in-depth explorations on the genes that are closely associated with the stress-resistant traits in rice. The existing rice SNP databases have made considerable contributions to rice genomic variation information but none of them have a particular focus on integrating stress-resistant variation and related phenotype data into one web resource. Results Rice Stress-Resistant SNP database () mainly focuses on SNPs specific to biotic and abiotic stress-resistant ability in rice, and presents them in a unified web resource platform. The Rice Stress-Resistant SNP (RSRS) database contains over 9.5 million stress-resistant SNPs and 797 stress-resistant candidate genes in rice, which were detected from more than 400 stress-resistant rice varieties. We incorporated the SNPs function, genome annotation and phenotype information into this database. Besides, the database has a user-friendly web interface for users to query, browse and visualize a specific SNP efficiently. RSRS database allows users to query the SNP information and their relevant annotations for individual variety or more varieties. The search results can be visualized graphically in a genome browser or displayed in formatted tables. Users can also align SNPs between two or more rice accessions. Conclusion RSRS database shows great utility for scientists to further characterize the function of variants related to environmental stress-resistant ability in rice.
Keyword ：
Abiotic stress Abiotic stress Biotic stress Biotic stress Database Database SNP SNP
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| GB/T 7714 | Tareke Woldegiorgis, Samuel , Wang, Shaobo , He, Yiruo et al. Rice Stress-Resistant SNP Database [J]. | RICE , 2019 , 12 (1) . |
| MLA | Tareke Woldegiorgis, Samuel et al. "Rice Stress-Resistant SNP Database" . | RICE 12 . 1 (2019) . |
| APA | Tareke Woldegiorgis, Samuel , Wang, Shaobo , He, Yiruo , Xu, Zhenhua , Chen, Lijuan , Tao, Huan et al. Rice Stress-Resistant SNP Database . | RICE , 2019 , 12 (1) . |
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