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学者姓名：牟少亮
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Abstract ：
Bacterial wilt, caused by Ralstonia solanacearum, is a devastating disease affecting plants in the Solanaceae family. In our previous study, CaHDZ27 was shown to act crucially in the pepper defense response to R. solanacearum. However, the molecular basis underlying CaHDZ27 function remains unexplored. In this study, we demonstrate that CaHDZ27 is post-translationally regulated by the 14-3-3 protein CaTFT7, which functions as a positive regulator in pepper immunity against R. solanacearum. RT-qPCR analysis revealed that CaTFT7 is transcriptionally induced by R. solanacearum infection. The data from virus-induced gene silencing revealed that CaTFT7 positively affects pepper immunity, which was further confirmed by the data of CaTFT7-overexpressing Nicotiana benthamiana. CaTFT7 interacted with CaHDZ27, thereby promoting the stability of CaHDZ27 and enhancing CaHDZ27 binding to the promoter of cysteine-rich receptor-like protein kinase 5 (CaCRK5), a gene that positively affects pepper defense against R. solanacearum. The above data indicated that CaTFT7 enhanced CaHDZ27 stability and promoted its ability to activate pepper immunity, shedding light on the mechanisms underlying pepper resistance to bacterial wilt.
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| GB/T 7714 | Mou, Shaoliang , Chen, Xiaodan , Cai, Jiao et al. The 14-3-3 protein CaTFT7 interacts with transcription factor CaHDZ27 to positively regulate pepper immunity against Ralstonia solanacearum [J]. | HORTICULTURE RESEARCH , 2025 , 12 (4) . |
| MLA | Mou, Shaoliang et al. "The 14-3-3 protein CaTFT7 interacts with transcription factor CaHDZ27 to positively regulate pepper immunity against Ralstonia solanacearum" . | HORTICULTURE RESEARCH 12 . 4 (2025) . |
| APA | Mou, Shaoliang , Chen, Xiaodan , Cai, Jiao , Zhang, Tingting , Luo, Tong , He, Shuilin . The 14-3-3 protein CaTFT7 interacts with transcription factor CaHDZ27 to positively regulate pepper immunity against Ralstonia solanacearum . | HORTICULTURE RESEARCH , 2025 , 12 (4) . |
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High temperature stress (HTS) is a serious threat to plant growth and development and to crop production in the context of global warming, and plant response to HTS is largely regulated at the transcriptional level by the actions of various transcription factors (TFs). However, whether and how homeodomain-leucine zipper (HD-Zip) TFs are involved in thermotolerance are unclear. Herein, we functionally characterized a pepper (Capsicum annuum) HD-Zip I TF CaHDZ15. CaHDZ15 expression was upregulated by HTS and abscisic acid in basal thermotolerance via loss- and gain-of-function assays by virus-induced gene silencing in pepper and overexpression in Nicotiana benthamiana plants. CaHDZ15 acted positively in pepper basal thermotolerance by directly targeting and activating HEAT SHOCK FACTORA6a (HSFA6a), which further activated CaHSFA2. In addition, CaHDZ15 interacted with HEAT SHOCK PROTEIN 70-2 (CaHsp70-2) and glyceraldehyde-3-phosphate dehydrogenase1 (CaGAPC1), both of which positively affected pepper thermotolerance. CaHsp70-2 and CaGAPC1 promoted CaHDZ15 binding to the promoter of CaHSFA6a, thus enhancing its transcription. Furthermore, CaHDZ15 and CaGAPC1 were protected from 26S proteasome-mediated degradation by CaHsp70-2 via physical interaction. These results collectively indicate that CaHDZ15, modulated by the interacting partners CaGAPC1 and CaHsp70-2, promotes basal thermotolerance by directly activating the transcript of CaHSFA6a. Thus, a molecular linkage is established among CaHsp70-2, CaGAPC1, and CaHDZ15 to transcriptionally modulate CaHSFA6a in pepper thermotolerance. The transcription factor CaHDZ15, modulated by cytoplasmic glyceraldehyde-3-phosphate dehydrogenase1 and heat shock protein 70-2, promotes pepper thermotolerance by activating HEAT SHOCK FACTORA6a.
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| GB/T 7714 | Mou, Shaoliang , He, Weihong , Jiang, Haitao et al. Transcription factor CaHDZ15 promotes pepper basal thermotolerance by activating HEAT SHOCK FACTORA6a [J]. | PLANT PHYSIOLOGY , 2024 , 195 (1) : 812-831 . |
| MLA | Mou, Shaoliang et al. "Transcription factor CaHDZ15 promotes pepper basal thermotolerance by activating HEAT SHOCK FACTORA6a" . | PLANT PHYSIOLOGY 195 . 1 (2024) : 812-831 . |
| APA | Mou, Shaoliang , He, Weihong , Jiang, Haitao , Meng, Qianqian , Zhang, Tingting , Liu, Zhiqin et al. Transcription factor CaHDZ15 promotes pepper basal thermotolerance by activating HEAT SHOCK FACTORA6a . | PLANT PHYSIOLOGY , 2024 , 195 (1) , 812-831 . |
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The authors reveal a mechanism in understanding the responses to Ralstonia solanacearum in pepper under high temperature and high humidity conditions, that is differential associations between transcription factors mediate distinct immune and heat responses. High temperature and high humidity (HTHH) conditions increase plant susceptibility to a variety of diseases, including bacterial wilt in solanaceous plants. Some solanaceous plant cultivars have evolved mechanisms to activate HTHH-specific immunity to cope with bacterial wilt disease. However, the underlying mechanisms remain poorly understood. Here we find that CaKAN3 and CaHSF8 upregulate and physically interact with each other in nuclei under HTHH conditions without inoculation or early after inoculation with R. solanacearum in pepper. Consequently, CaKAN3 and CaHSF8 synergistically confer immunity against R. solanacearum via activating a subset of NLRs which initiates immune signaling upon perception of unidentified pathogen effectors. Intriguingly, when HTHH conditions are prolonged without pathogen attack or the temperature goes higher, CaHSF8 no longer interacts with CaKAN3. Instead, it directly upregulates a subset of HSP genes thus activating thermotolerance. Our findings highlight mechanisms controlling context-specific activation of high-temperature-specific pepper immunity and thermotolerance mediated by differential CaKAN3-CaHSF8 associations.
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| GB/T 7714 | Yang, Sheng , Cai, Weiwei , Wu, Ruijie et al. Differential CaKAN3-CaHSF8 associations underlie distinct immune and heat responses under high temperature and high humidity conditions [J]. | NATURE COMMUNICATIONS , 2023 , 14 (1) . |
| MLA | Yang, Sheng et al. "Differential CaKAN3-CaHSF8 associations underlie distinct immune and heat responses under high temperature and high humidity conditions" . | NATURE COMMUNICATIONS 14 . 1 (2023) . |
| APA | Yang, Sheng , Cai, Weiwei , Wu, Ruijie , Huang, Yu , Lu, Qiaoling , Huang, Xueying et al. Differential CaKAN3-CaHSF8 associations underlie distinct immune and heat responses under high temperature and high humidity conditions . | NATURE COMMUNICATIONS , 2023 , 14 (1) . |
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Background Cysteine-rich receptor-like kinases (CRKs) represent a large subfamily of receptor-like kinases and play vital roles in diverse physiological processes in regulating plant growth and development. Results CaCRK5 transcripts were induced in pepper upon the infection of Ralstonia solanacearum and treatment with salicylic acid. The fusions between CaCRK5 and green fluorescence protein were targeted to the plasma membrane. Suppression of CaCRK5 via virus-induced gene silencing (VIGS) made pepper plants significantly susceptible to R. solanacearum infection, which was accompanied with decreased expression of defense related genes CaPR1, CaSAR8.2, CaDEF1 and CaACO1. Overexpression of CaCRK5 increased resistance against R. solanacearum in Nicotiana benthamiana. Furthermore, electrophoretic mobility shift assay and chromatin immunoprecipitation coupled with quantitative real-time PCR analysis revealed that a homeodomain zipper I protein CaHDZ27 can active the expression of CaCRK5 through directly binding to its promoter. Yeast two-hybrid and bimolecular fluorescence complementation (BiFC) analyses suggested that CaCRK5 heterodimerized with the homologous member CaCRK6 on the plasma membrane. Conclusions Our data revealed that CaCRK5 played a positive role in regulating immune responses against R. solanacearum infection in pepper.
Keyword ：
CaCRK5 CaCRK5 Capsicum annuum Capsicum annuum Immune response Immune response Ralstonia solanacearum Ralstonia solanacearum
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| GB/T 7714 | Mou, Shaoliang , Meng, Qianqian , Gao, Feng et al. A cysteine-rich receptor-like protein kinase CaCKR5 modulates immune response against Ralstonia solanacearum infection in pepper [J]. | BMC PLANT BIOLOGY , 2021 , 21 (1) . |
| MLA | Mou, Shaoliang et al. "A cysteine-rich receptor-like protein kinase CaCKR5 modulates immune response against Ralstonia solanacearum infection in pepper" . | BMC PLANT BIOLOGY 21 . 1 (2021) . |
| APA | Mou, Shaoliang , Meng, Qianqian , Gao, Feng , Zhang, Tingting , He, Weihong , Guan, Deyi et al. A cysteine-rich receptor-like protein kinase CaCKR5 modulates immune response against Ralstonia solanacearum infection in pepper . | BMC PLANT BIOLOGY , 2021 , 21 (1) . |
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生物化学是生命科学领域一门重要的专业基础课,但因其知识点多、理论性强、且知识点与相关技术更新较快,如何打破传统教学的框架,开展有效的教学改革具有较大的现实意义。本文探讨了科研实践在教学改革中的作用,并深入分析了科研实践如何与教学相互促进,有助于提高生物化学的教学效率。
Keyword ：
教学改革 教学改革 生物化学 生物化学 科研实践 科研实践
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| GB/T 7714 | 张灵玲 , 牟少亮 , 艾育芳 et al. 科研实践与生物化学教学相长的体会 [J]. | 生命的化学 , 2020 , 40 (12) : 2303-2306 . |
| MLA | 张灵玲 et al. "科研实践与生物化学教学相长的体会" . | 生命的化学 40 . 12 (2020) : 2303-2306 . |
| APA | 张灵玲 , 牟少亮 , 艾育芳 , 马洪丽 . 科研实践与生物化学教学相长的体会 . | 生命的化学 , 2020 , 40 (12) , 2303-2306 . |
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BackgroundBacterial wilt caused by Ralstonia solanacearum is one of the most important diseases in pepper worldwide, however, the molecular mechanism underlying pepper resistance to bacterial wilt remains poorly understood.ResultsHerein, a novel RD leucine-rich repeat receptor-like kinase, CaLRR-RLK1, was functionally characterized in immunity against R. solanacearum. CaLRR-RLK1 was targeted exclusively to plasma membrane and was up-regulated by R. solanacearum inoculation (RSI) as well as by the exogenous application of salicylic acid (SA), methyl jasmonate (MeJA) or ethephon (ETH). The silencing of CaLRR-RLK1 led to enhanced susceptibility of pepper plants to RSI, accompanied by down-regulation of immunity-related genes including CaACO1, CaHIR1, CaPR4 and CaPO2. In contrast, transient overexpression of CaLRR-RLK1 triggered hypersensitive response (HR)-like cell death and H2O2 accumulation in pepper leaves, manifested by darker trypan blue and DAB staining respectively. In addition, the ectopic overexpression of CaLRR-RLK1 in tobacco plants enhanced resistance R. solanacearum, accompanied with the immunity associated marker genes including NtPR2, NtPR2, NtHSR203 and NtHSR515. Furthermore, it was found that CaHDZ27, a positive regulator in pepper response to RSI in our previous study, transcriptionally activated CaLRR-RLK1 by direct targeting its promoter probably in a CAATTATTG dependent manner.ConclusionThe study revealed that CaLRR-RLK1 confers pepper resistance to R. solanacearum as the direct targeting of CaHDZ27.
Keyword ：
CaHDZ27 CaHDZ27 CaLRR-RLK1 CaLRR-RLK1 Pepper Pepper Plant immunity Plant immunity Ralstonia solanacearum Ralstonia solanacearum
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| GB/T 7714 | Mou, Shaoliang , Gao, Feng , Shen, Lei et al. CaLRR-RLK1, a novel RD receptor-like kinase from Capsicum annuum and transcriptionally activated by CaHDZ27, act as positive regulator in Ralstonia solanacearum resistance [J]. | BMC PLANT BIOLOGY , 2019 , 19 . |
| MLA | Mou, Shaoliang et al. "CaLRR-RLK1, a novel RD receptor-like kinase from Capsicum annuum and transcriptionally activated by CaHDZ27, act as positive regulator in Ralstonia solanacearum resistance" . | BMC PLANT BIOLOGY 19 (2019) . |
| APA | Mou, Shaoliang , Gao, Feng , Shen, Lei , Yang, Sheng , He, Weihong , Cheng, Wei et al. CaLRR-RLK1, a novel RD receptor-like kinase from Capsicum annuum and transcriptionally activated by CaHDZ27, act as positive regulator in Ralstonia solanacearum resistance . | BMC PLANT BIOLOGY , 2019 , 19 . |
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前人研究发现水稻Os ERF96(ethylene responsive factor 96)可应答白叶枯病病原菌的侵染,但其功能及表达调控机制仍不清楚,本研究进一步分析了该基因在水稻应答稻瘟病病原菌侵染及外源激素(SA和Me JA)处理下的转录情况,并分析了其启动子的诱导表达活性。结果表明:相对于对照组,Os ERF96在接种稻瘟病后1~4 d表达量显著上调,以第1天最高,此后逐渐下降,外源SA处理后Os ERF96表达量持续上调;利用Os ERF96启动子驱动GUS的转基因株系分析了Os ERF96启动子的诱导活性,结果表明GUS在根、茎和叶均有不同程度组成型表达,稻瘟病菌接种后4~7 d GUS活性持续升高。GUS活性定量表明,稻瘟病菌和SA处理条件下均出现了升高。综上所述,Os ERF96可应答白叶枯病或稻瘟病病原菌的浸染,其启动子是一个对病原菌侵染产生应答的诱导性启动子。
Keyword ：
OsERF96 OsERF96 启动子 启动子 水稻 水稻 稻瘟病菌 稻瘟病菌
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| GB/T 7714 | 牟少亮 , 申磊 , 石星辰 et al. 水稻OsERF96应答病原菌的表达及启动子的功能分析 [J]. | 植物遗传资源学报 , 2017 , 18 (01) : 133-138 . |
| MLA | 牟少亮 et al. "水稻OsERF96应答病原菌的表达及启动子的功能分析" . | 植物遗传资源学报 18 . 01 (2017) : 133-138 . |
| APA | 牟少亮 , 申磊 , 石星辰 , 官德义 , 何水林 . 水稻OsERF96应答病原菌的表达及启动子的功能分析 . | 植物遗传资源学报 , 2017 , 18 (01) , 133-138 . |
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The leucine-rich repeat (LRR) proteins play important roles in the recognition of corresponding ligands and signal transduction networks in plant defence responses. Herein, a novel LRR protein from Capsicum annuum, CaLRR51, was identified and characterized. It was localized to the plasma membrane and transcriptionally up-regulated by Ralstonia solanacearum infection (RSI), as well as the exogenous application of salicylic acid (SA), jasmonic acid (JA) and ethephon (ETH). Virus-induced gene silencing of CaLRR51 significantly increased the susceptibility of pepper to RSI. By contrast, transient overexpression of CaLRR51 in pepper plants activated hypersensitive response (HR)-like cell death, and up-regulated the defence-related marker genes, including PO2, HIR1, PR1, DEF1 and ACO1. Moreover, ectopic overexpression of CaLRR51 in transgenic tobacco plants significantly enhanced the resistance to RSI. Transcriptional expression of the corresponding defence-related marker genes in transgenic tobacco plants was also found to be enhanced by the overexpression of CaLRR51, which was potentiated by RSI. These loss- and gain-of-function assays suggest that CaLRR51 acts as a positive regulator in the response of pepper to RSI. In addition, the putative signal peptide and transmembrane region were found to be required for plasma membrane targeting of CaLRR51, which is indispensable for the role of CaLRR51 in plant immunity.
Keyword ：
Capsicum annuum Capsicum annuum leucine-rich repeat (LRR) protein leucine-rich repeat (LRR) protein plant defence response plant defence response Ralstonia solanacearum Ralstonia solanacearum
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| GB/T 7714 | Cheng, Wei , Xiao, Zhuoli , Cai, Hanyang et al. A novel leucine-rich repeat protein, CaLRR51, acts as a positive regulator in the response of pepper to Ralstonia solanacearum infection [J]. | MOLECULAR PLANT PATHOLOGY , 2017 , 18 (8) : 1089-1100 . |
| MLA | Cheng, Wei et al. "A novel leucine-rich repeat protein, CaLRR51, acts as a positive regulator in the response of pepper to Ralstonia solanacearum infection" . | MOLECULAR PLANT PATHOLOGY 18 . 8 (2017) : 1089-1100 . |
| APA | Cheng, Wei , Xiao, Zhuoli , Cai, Hanyang , Wang, Chuanqing , Hu, Yang , Xiao, Yueping et al. A novel leucine-rich repeat protein, CaLRR51, acts as a positive regulator in the response of pepper to Ralstonia solanacearum infection . | MOLECULAR PLANT PATHOLOGY , 2017 , 18 (8) , 1089-1100 . |
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Homeodomain-leucine zipper class I (HD-Zip I) transcription factors have been functionally characterized in plant responses to abiotic stresses, but their roles in plant immunity are poorly understood. Here, a HD-Zip I gene, CaHZ27, was isolated from pepper (Capsicum annum) and characterized for its role in pepper immunity. Quantitative real-time polymerase chain reaction showed that CaHDZ27 was transcriptionally induced by Ralstonia solanacearum inoculation and exogenous application of methyl jasmonate, salicylic acid, or ethephon. The CaHDZ27-green fluorescent protein fused protein was targeted exclusively to the nucleus. Chromatin immunoprecipitation demonstrated that CaHDZ27 bound to the 9-bp pseudopalindromic element (CAATAATTG) and triggered beta-glucuronidase expression in a CAATAATTG-dependent manner. Virus-induced gene silencing of CaHDZ27 significantly attenuated the resistance of pepper plants against R. solanacearum and downregulated defense-related marker genes, including CaHIR1, CaACO1, CaPR1, CaPR4, CaPO2, and CaBPR1. By contrast, transient overexpression of CaHDZ27 triggered strong cell death mediated by the hypersensitive response and upregulated the tested immunity-associated marker genes. Ectopic CaHDZ27 expression in tobacco enhances its resistance against R. solanacearum. These results collectively suggest that CaHDZ27 functions as a positive regulator in pepper resistance against R. solanacearum. Bimolecular fluorescence complementation and coimmunoprecipitation assays indicate that CaHDZ27 monomers bind with each other, and this binding is enhanced significantly by R. solanacearum inoculation. We speculate that homodimerization of CaHZ27 might play a role in pepper response to R. solanacearum, further direct evidence is required to confirm it.
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| GB/T 7714 | Mou, Shaoliang , Liu, Zhiqin , Gao, Feng et al. CaHDZ27, a Homeodomain-Leucine Zipper I Protein, Positively Regulates the Resistance to Ralstonia solanacearum Infection in Pepper [J]. | MOLECULAR PLANT-MICROBE INTERACTIONS , 2017 , 30 (12) : 960-973 . |
| MLA | Mou, Shaoliang et al. "CaHDZ27, a Homeodomain-Leucine Zipper I Protein, Positively Regulates the Resistance to Ralstonia solanacearum Infection in Pepper" . | MOLECULAR PLANT-MICROBE INTERACTIONS 30 . 12 (2017) : 960-973 . |
| APA | Mou, Shaoliang , Liu, Zhiqin , Gao, Feng , Yang, Sheng , Su, Meixia , Shen, Lei et al. CaHDZ27, a Homeodomain-Leucine Zipper I Protein, Positively Regulates the Resistance to Ralstonia solanacearum Infection in Pepper . | MOLECULAR PLANT-MICROBE INTERACTIONS , 2017 , 30 (12) , 960-973 . |
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CabZIP63, indirectly activated by CaWRKY40, positively modulates transcription of CabZIP63 and CaWRKY40, enhances the binding of CaWRKY40 to its target promoters, and, therefore, increases resistance to Ralstonia solanacearum and thermotolerance.CaWRKY40 is known to act as a positive regulator in the response of pepper (Capsicum annuum) to Ralstonia solanacearum inoculation (RSI) or high temperature-high humidity (HTHH), but the underlying mechanism remains elusive. Herein, we report that CabZIP63, a pepper bZIP family member, participates in this process by regulating the expression of CaWRKY40. CabZIP63 was found to localize in the nuclei, be up-regulated by RSI or HTHH, bind to promoters of both CabZIP63 (pCabZIP63) and CaWRKY40 (pCaWRKY40), and activate pCabZIP63- and pCaWRKY40-driven beta-glucuronidase expression in a C- or G-box-dependent manner. Silencing of CabZIP63 by virus-induced gene silencing (VIGS) in pepper plants significantly attenuated their resistance to RSI and tolerance to HTHH, accompanied by down-regulation of immunity- or thermotolerance-associated CaPR1, CaNPR1, CaDEF1, and CaHSP24. Hypersensitive response-mediated cell death and expression of the tested immunity- and thermotolerance-associated marker genes were induced by transient overexpression (TOE) of CabZIP63, but decreased by that of CabZIP63-SRDX. Additionally, binding of CabZIP63 to pCaWRKY40 was up-regulated by RSI or HTHH, and the transcript level of CaWRKY40 and binding of CaWRKY40 to the promoters of CaPR1, CaNPR1, CaDEF1 and CaHSP24 were up-regulated by TOE of CabZIP63. On the other hand, CabZIP63 was also up-regulated transcriptionally by TOE of CaWRKY40. The data suggest collectively that CabZIP63 directly or indirectly regulates the expression of CaWRKY40 at both the transcriptional and post-transcriptional level, forming a positive feedback loop with CaWRKY40 during pepper's response to RSI or HTHH. Altogether, our data will help to elucidate the underlying mechanism of crosstalk between pepper's response to RSI and HTHH.
Keyword ：
CabZIP63 CabZIP63 CaWRKY40 CaWRKY40 high temperature-high humidity high temperature-high humidity pepper pepper Ralstonia solanacearum Ralstonia solanacearum transcription factors transcription factors
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| GB/T 7714 | Shen, Lei , Liu, Zhiqin , Yang, Sheng et al. Pepper CabZIP63 acts as a positive regulator during Ralstonia solanacearum or high temperature-high humidity challenge in a positive feedback loop with CaWRKY40 [J]. | JOURNAL OF EXPERIMENTAL BOTANY , 2016 , 67 (8) : 2439-2451 . |
| MLA | Shen, Lei et al. "Pepper CabZIP63 acts as a positive regulator during Ralstonia solanacearum or high temperature-high humidity challenge in a positive feedback loop with CaWRKY40" . | JOURNAL OF EXPERIMENTAL BOTANY 67 . 8 (2016) : 2439-2451 . |
| APA | Shen, Lei , Liu, Zhiqin , Yang, Sheng , Yang, Tong , Liang, Jiaqi , Wen, Jiayu et al. Pepper CabZIP63 acts as a positive regulator during Ralstonia solanacearum or high temperature-high humidity challenge in a positive feedback loop with CaWRKY40 . | JOURNAL OF EXPERIMENTAL BOTANY , 2016 , 67 (8) , 2439-2451 . |
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