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学者姓名：杨燕凌
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Abstract ：
Aspergillus flavus and its secondary metabolites aflatoxins pose a significant threat to the health of humans, animals, and plants. Therefore, there is an urgent need to control A. flavus contamination. AfverB plays a key role in the aflatoxin gene cluster; however, its function and mechanism in fungal development and virulence remain poorly understood. In this study, we constructed afVerB gene deletion mutants (triangle afVerB-1 and triangle afVerB-2) and two CYP domain mutants (afVerB(triangle D1) and afVerB(triangle D2)) through homologous recombination. Phenotype analysis revealed that, via its two CYP domains, AfVerB is deeply involved in fungal morphogenesis and aflatoxin synthesis. Insect and crop colonization models revealed that AfVerB plays a key role in the fungus's ability to infect hosts, and stress experiments discovered that AfVerB plays a significant role in the response to various environmental stresses, which explains why AfVerB is a key factor in fungal infection to some extent. RT-qPCR analysis demonstrated that AfVerB performs its bio-function through corresponding regulatory factors. We ultimately discovered that AfVerB is deeply involved in cell membrane stress stability, thereby participating in the regulation of fungal drug resistance (sensitive to AMB and resistant to VOR in this study). The CYP domain of AfVerB, particularly its second CYP domain, is crucial for the execution of its biological functions. This study elucidated the regulatory mechanisms by which AfVerB regulates fungal pathogenicity and aflatoxin biosynthesis, providing potential strategies for controlling A. flavus and its aflatoxin contamination.
Keyword ：
AFB(1) AFB(1) AfVerB AfVerB Aspergillus flavus Aspergillus flavus CYP domain CYP domain fungal drug resistance fungal drug resistance
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| GB/T 7714 | Ye, Kangfu , Zhou, Song , Wu, Dandan et al. Molecular Mechanism of Aflatoxin B1 Synthesis Related AfVerB Regulating the Development, AFB1 Biosyntheis and Virulence of Aspergillus flavus Mainly Through Its CYP Domain [J]. | JOURNAL OF FUNGI , 2025 , 11 (4) . |
| MLA | Ye, Kangfu et al. "Molecular Mechanism of Aflatoxin B1 Synthesis Related AfVerB Regulating the Development, AFB1 Biosyntheis and Virulence of Aspergillus flavus Mainly Through Its CYP Domain" . | JOURNAL OF FUNGI 11 . 4 (2025) . |
| APA | Ye, Kangfu , Zhou, Song , Wu, Dandan , Ma, Dongmei , Yao, Yanfang , Yang, Chi et al. Molecular Mechanism of Aflatoxin B1 Synthesis Related AfVerB Regulating the Development, AFB1 Biosyntheis and Virulence of Aspergillus flavus Mainly Through Its CYP Domain . | JOURNAL OF FUNGI , 2025 , 11 (4) . |
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The epigenetic reader SntB was identified as an important transcriptional regulator of growth, development, and secondary metabolite synthesis in Aspergillus flavus. However, the underlying molecular mechanism is still unclear. In this study, by gene deletion and complementation, we found SntB is essential for mycelia growth, conidial production, sclerotia formation, aflatoxin synthesis, and host colonization. Chromatin immunoprecipitation sequencing (ChIP-seq) and RNA sequencing (RNA-seq) analysis revealed that SntB played key roles in oxidative stress response of A. flavus, influencing related gene activity, especially catC encoding catalase. SntB regulated the expression activity of catC with or without oxidative stress, and was related to the expression level of the secretory lipase (G4B84_008359). The deletion of catC showed that CatC participated in the regulation of fungal morphogenesis, reactive oxygen species (ROS) level, and aflatoxin production, and that CatC significantly regulated fungal sensitive reaction and AFB1 yield under oxidative stress. Our study revealed the potential machinery that SntB regulated fungal morphogenesis, mycotoxin anabolism, and fungal virulence through the axle of from H3K36me3 modification to fungal virulence and mycotoxin biosynthesis. The results of this study shed light into the SntB-mediated transcript regulation pathways of fungal mycotoxin anabolism and virulence, which provided potential strategy to control the contamination of A. flavus and its aflatoxins.
Keyword ：
Aspergillus flavus Aspergillus flavus CatC CatC ChIP-seq ChIP-seq Other Other RNA-seq RNA-seq SntB SntB
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| GB/T 7714 | Wu, Dandan , Yang, Chi , Yao, Yanfang et al. SntB triggers the antioxidant pathways to regulate development and aflatoxin biosynthesis in Aspergillus flavus [J]. | ELIFE , 2024 , 13 . |
| MLA | Wu, Dandan et al. "SntB triggers the antioxidant pathways to regulate development and aflatoxin biosynthesis in Aspergillus flavus" . | ELIFE 13 (2024) . |
| APA | Wu, Dandan , Yang, Chi , Yao, Yanfang , Ma, Dongmei , Lin, Hong , Hao, Ling et al. SntB triggers the antioxidant pathways to regulate development and aflatoxin biosynthesis in Aspergillus flavus . | ELIFE , 2024 , 13 . |
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As a filamentous pathogenic fungus with high-yield of aflatoxin B1, Aspergillus flavus is commonly found in various agricultural products. It is crucial to develop effective strategies aimed at the prevention of the contamination of A. flavus and aflatoxin. Hexokinase AfHxk1 is a critical enzyme in fungal glucose metabolism. However, the role of AfHxk1 in A. flavus development, aflatoxin biosynthesis, and virulence has not yet been explored. In this study, afHxk1 gene deletion mutant (Delta afHxk1), complementary strain (Com-afHxk1), and the domain deletion strains (afHxk1(Delta D1) and afHxk1(Delta D2)) were constructed by homologous recombination. Phenotype study and RT-qPCR revealed that AfHxk1 upregulates mycelium growth and spore and sclerotia formation, but down regulates AFB1 biosynthesis through related classical signaling pathways. Invading models and environmental stress analysis revealed that through involvement in carbon source utilization, conidia germination, and the sensitivity response of A. flavus to a series of environmental stresses, AfHxk1 deeply participates in the regulation of pathogenicity of A. flavus to crop kernels and Galleria mellonella larvae. The construction of domain deletion strains, afHxk1(Delta D1) and afHxk1(Delta D2), further revealed that AfHxk1 regulates the morphogenesis, mycotoxin biosynthesis, and the fungal pathogenicity mainly through its domain, Hexokinase_2. The results of this study revealed the biological role of AfHxk1 in Aspergillus spp., and might provide a novel potential target for the early control of the contamination of A. flavus.
Keyword ：
AFB(1) AFB(1) AfHxk1 AfHxk1 Aspergillus flavus Aspergillus flavus crop kernels crop kernels Galleria mellonella Galleria mellonella
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| GB/T 7714 | Huang, Zongting , Wu, Dandan , Yang, Sile et al. Regulation of Fungal Morphogenesis and Pathogenicity of Aspergillus flavus by Hexokinase AfHxk1 through Its Domain Hexokinase_2 [J]. | JOURNAL OF FUNGI , 2023 , 9 (11) . |
| MLA | Huang, Zongting et al. "Regulation of Fungal Morphogenesis and Pathogenicity of Aspergillus flavus by Hexokinase AfHxk1 through Its Domain Hexokinase_2" . | JOURNAL OF FUNGI 9 . 11 (2023) . |
| APA | Huang, Zongting , Wu, Dandan , Yang, Sile , Fu, Wangzhuo , Ma, Dongmei , Yao, Yanfang et al. Regulation of Fungal Morphogenesis and Pathogenicity of Aspergillus flavus by Hexokinase AfHxk1 through Its Domain Hexokinase_2 . | JOURNAL OF FUNGI , 2023 , 9 (11) . |
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As a widely distributed food-borne pathogenic fungus, Aspergillus flavus and its secondary metabolites, mainly aflatoxin B1 (AFB1), pose a great danger to humans. It is urgent to reveal the complex regulatory network of toxigenic and virulence of this fungus. The bio-function of Set9, a SET-domain-containing histone methyltransferase, is still unknown in A. flavus. By genetic engineering means, this study revealed that, through catalyzing H4K20me2 and-me3, Set9 is involved in fungal growth, reproduction, and mycotoxin production via the orthodox regulation pathway, and regulates fungal colonization on crop kernels through adjusting fungal sensitivity reactions to oxidation stress and cell wall integrity stress. Further domain deletion and point mutation inferred that the SET domain is the core element in catalyzing H4K20 methylation, and D200 site of the domain is the key amino acid in the active center of the methyltransferase. Combined with RNA-seq analysis, this study revealed that Set9 regulates the aflatoxin gene cluster by the AflR-like protein (ALP), other than traditional AflR. This study revealed the epigenetic regulation mechanism of fungal morphogenesis, secondary metabolism, and pathogenicity of A. flavus mediated by the H4K20-methyltransferase Set9, which might provide a potential new target for early prevention of contamination of A. flavus and its deadly mycotoxins.
Keyword ：
AFB 1 AFB 1 Aspergillus flavus Aspergillus flavus Epigenetic regulation Epigenetic regulation Histone methyltransferase Histone methyltransferase Set9 Set9
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| GB/T 7714 | Hao, Ling , Zhang, Mengjuan , Yang, Chi et al. The epigenetic regulator Set9 harmonizes fungal development, secondary metabolism, and colonization capacity of Aspergillus flavus [J]. | INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY , 2023 , 403 . |
| MLA | Hao, Ling et al. "The epigenetic regulator Set9 harmonizes fungal development, secondary metabolism, and colonization capacity of Aspergillus flavus" . | INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY 403 (2023) . |
| APA | Hao, Ling , Zhang, Mengjuan , Yang, Chi , Pan, Xiaohua , Wu, Dandan , Lin, Hong et al. The epigenetic regulator Set9 harmonizes fungal development, secondary metabolism, and colonization capacity of Aspergillus flavus . | INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY , 2023 , 403 . |
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Aspergillus flavus and its main secondary metabolite AFB1 pose a serious threat to several important crops worldwide. Recently, it has been reported that some PHD family transcription factors are involved in the morphogenesis and AFB1 biological synthesis in A. flavus, but the role of Cti6, a PHD domain containing protein in A. flavus, is totally unknown. The study was designed to reveal the biological function of Cti6 in the fungus by deletion of cti6, and its two domains (PHD and Atrophin-1) through homologous recombination, respectively. The results showed that Cti6 might up-regulate the mycelium growth, conidiation, sclerotia formation and AFB1 biological synthesis of A. flavus by its PHD domain, while Atrophin-1 also improved the conidiation of the fungus. The qRT-PCR analysis showed that Cti6 increased the conidiation of the fungus through AbaA and BrlA mediated conidiation pathway, triggered the formation of sclerotia by orthodox sclerotia formation pathway, and improved the production of AFB1 by orthodox AFB1 synthesis pathway. Crops models analysis showed that A. flavus Cti6 plays vital role in colonization and the production of AFB1 on the host grains mainly via PHD domain. Bioinformatics analysis showed Cti6 is conservative in Aspergillus spp., and mCherry mediated subcellular localization showed that most Cti6 accumulated in the nuclei, which reflected that Cti6 performed its important biological function in the nuclei in Aspergillus spp.. The results of the current study elucidate the roles of PHD domain containing proteins in the mechanism of the infection of crops by A. flavus, and provided a novel target for effectively controlling the contamination of Aspergillus spp. to crops.
Keyword ：
AFB1 AFB1 Aspergillus flavus Aspergillus flavus Colonization Colonization Cti6 Cti6 PHD domain PHD domain
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| GB/T 7714 | Mengjuan, Zhang , Guanglan, Lin , Xiaohua, Pan et al. The PHD transcription factor Cti6 is involved in the fungal colonization and aflatoxin B1 biological synthesis of Aspergillus flavus [J]. | IMA FUNGUS , 2021 , 12 (1) . |
| MLA | Mengjuan, Zhang et al. "The PHD transcription factor Cti6 is involved in the fungal colonization and aflatoxin B1 biological synthesis of Aspergillus flavus" . | IMA FUNGUS 12 . 1 (2021) . |
| APA | Mengjuan, Zhang , Guanglan, Lin , Xiaohua, Pan , Weitao, Song , Can, Tan , Xuan, Chen et al. The PHD transcription factor Cti6 is involved in the fungal colonization and aflatoxin B1 biological synthesis of Aspergillus flavus . | IMA FUNGUS , 2021 , 12 (1) . |
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Abstract ：
植物学是农林高校重要的公共基础课,建立合理的课程思政教学模式,将有力地推动植物学课程与思政课程融合,全面提升学生的综合素质。本文在总结分析教学经验、典型教学案例的基础上,提出了"一个理念、两大目标、三项保障、四个措施"的植物学课程思政1234教学模式,并取得了良好的教学效果,为切实发挥植物学课程的育人功能奠定基础。
Keyword ：
实践 实践 教学模式 教学模式 植物学 植物学 课程思政 课程思政
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| GB/T 7714 | 陈煜 , 林玲 , 杨燕凌 . 植物学课程思政的教学模式与实践 [J]. | 佳木斯职业学院学报 , 2020 , 36 (08) : 125-126 . |
| MLA | 陈煜 et al. "植物学课程思政的教学模式与实践" . | 佳木斯职业学院学报 36 . 08 (2020) : 125-126 . |
| APA | 陈煜 , 林玲 , 杨燕凌 . 植物学课程思政的教学模式与实践 . | 佳木斯职业学院学报 , 2020 , 36 (08) , 125-126 . |
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植物生理生化实验课是培养学生创新能力的重要课程。将雨课堂与翻转课堂PBL教学模式进行有机的融合,对课前、课中和课后3个环节进行设计与实施,以学生为主体,使雨课堂成为师生互动的有效桥梁,将课程内容扩展到实际生产应用中的热点问题和科学前沿问题。结果表明:学生的学习热情、学习主动性和学习成绩得到了显著的提高,学生发现问题、分析问题、解决问题和创新意识等科研素养也得到了明显提高。
Keyword ：
植物生理生化实验 植物生理生化实验 科研素养 科研素养 翻转课堂 翻转课堂 雨课堂 雨课堂
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| GB/T 7714 | 张惠莹 , 刘建 , 连玲丽 et al. 基于雨课堂的植物生理生化实验课PBL教学探索 [J]. | 安徽农学通报 , 2019 , 25 (17) : 129-132 . |
| MLA | 张惠莹 et al. "基于雨课堂的植物生理生化实验课PBL教学探索" . | 安徽农学通报 25 . 17 (2019) : 129-132 . |
| APA | 张惠莹 , 刘建 , 连玲丽 , 杨燕凌 . 基于雨课堂的植物生理生化实验课PBL教学探索 . | 安徽农学通报 , 2019 , 25 (17) , 129-132 . |
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Numerous studies have focused on the mechanism of aflatoxin B1 (AFB1) metabolism and its carcinogenic mechanism, but how AFB1 is transported into hepatocytes and how it is transferred inside hepatocytes remains unknown. In this study, the AFB1-interacting protein, estradiol 17 beta-dehydrogenase 5 (Akr1c6), was identified with an immobilized affinity chromatography technique and LC-MS/MS. The interaction between Akr1c6 and AFB1 was confirmed with ELISA, and the results showed that Akr1c6 could efficiently bind AFB1. Anti-Akr1c6 polyclonal antibody from rabbit was prepared, and the IC50 values of AFB1 to BRL (normal big rat liver cells) and NRK (normal rat kidney cells) were detected using the MTT assay. It was found that the Akr1c6 expression level in BRL was significantly affected under the IC50 value of AFB1 (P < 0.05), but no obvious expression difference of Akr1c6 was observed in NRK. This suggested that Akr1c6 in liver cells participates in the transportation and/or metabolism of AFB1, and though Akr1c6 was expressed in the kidneys, it did not play a role in AFB1 transportation or metabolism. The conclusions of this study lay a foundation for further exploring the role of AFB1 binding proteins in the toxicology of AFB1 to hepatocytes and the pathway through which AFB1 enters hepatocytes and their nuclei.
Keyword ：
Aflatoxin B1-binding protein Aflatoxin B1-binding protein estradiol 17 beta-dehydrogenase 5 estradiol 17 beta-dehydrogenase 5 hepatocytes hepatocytes toxicology of AFB1 toxicology of AFB1
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| GB/T 7714 | Yang, Yanling , Huang, Yaling , Zeng, Linmao et al. Estradiol 17 beta-dehydrogenase 5 is involved in aflatoxin B1 transportation [J]. | TURKISH JOURNAL OF VETERINARY & ANIMAL SCIENCES , 2017 , 41 (4) : 482-489 . |
| MLA | Yang, Yanling et al. "Estradiol 17 beta-dehydrogenase 5 is involved in aflatoxin B1 transportation" . | TURKISH JOURNAL OF VETERINARY & ANIMAL SCIENCES 41 . 4 (2017) : 482-489 . |
| APA | Yang, Yanling , Huang, Yaling , Zeng, Linmao , Gao, Yuanyuan , Li, Yu , Ji, Yanguang et al. Estradiol 17 beta-dehydrogenase 5 is involved in aflatoxin B1 transportation . | TURKISH JOURNAL OF VETERINARY & ANIMAL SCIENCES , 2017 , 41 (4) , 482-489 . |
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A method using immobilized affinity chromatography (IAC) was developed to screen for aflatoxin B1 (AFB1)-binding proteins. AFB1 and bovine serum albumin (BSA) coupled protein (BSA-AFB1) was prepared using 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride. The resulting coupled compound was immobilized onto PVDF transfer membranes, which were then incubated with total protein from mouse liver. AFB1-binding proteins were eluted, after non-specific washing, by specific elution, and the eluted proteins were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Two candidate AFB1-binding proteins were identified by liquid chromatography-tandem mass spectrometry as the 40S ribosomal protein SA (RPSA) and a putative uncharacterized protein. RPSA and AFB1 interactions were further analyzed by ELISA in vitro and laser confocal immunofluorescence analysis in vivo. The results from ELISA and immunofluorescence showed that RPSA efficiently bound AFB1 in vitro and in vivo. This study's conclusion laid the foundation for further exploration of the role of AFB1-binding proteins in AFB1 toxicology towards hepatocytes and the entry pathway of AFB1 into hepatocytes. (C) 2015 Elsevier B.V. All rights reserved.
Keyword ：
AFB1-binding proteins AFB1-binding proteins ELISA ELISA IAC IAC Immunofluorescence Immunofluorescence RPSA RPSA
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| GB/T 7714 | Zhuang, Zhenhong , Huang, Yaling , Yang, Yanling et al. Identification of AFB1-interacting proteins and interactions between RPSA and AFB1 [J]. | JOURNAL OF HAZARDOUS MATERIALS , 2016 , 301 : 297-303 . |
| MLA | Zhuang, Zhenhong et al. "Identification of AFB1-interacting proteins and interactions between RPSA and AFB1" . | JOURNAL OF HAZARDOUS MATERIALS 301 (2016) : 297-303 . |
| APA | Zhuang, Zhenhong , Huang, Yaling , Yang, Yanling , Wang, Shihua . Identification of AFB1-interacting proteins and interactions between RPSA and AFB1 . | JOURNAL OF HAZARDOUS MATERIALS , 2016 , 301 , 297-303 . |
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为了通过诱变筛选获得豆豉溶栓酶高酶活菌株。研究以豆豉溶栓酶产生菌株Bacillus subtilis LD-8547为出发菌株,分别通过紫外线诱变和硫酸二乙酯的复合诱变,根据奶粉平板和血粉平板上菌落透明圈的大小进行初筛和复筛。并采用四肽底物测定法进行了溶栓酶的酶活力测定。结果表明,通过实验获得了产豆豉溶栓酶酶活力达18 228 U/mL的LD-8547-25菌株,比诱变出发菌株的酶活力提高了107%。为豆豉溶栓酶高酶活菌株的诱变筛选提供了有益的试验数据。
Keyword ：
溶栓酶 溶栓酶 硫酸二乙酯 硫酸二乙酯 紫外诱变 紫外诱变 诱变育种 诱变育种
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| GB/T 7714 | 袁军 , 李国良 , 沈榕强 et al. 豆豉溶栓酶产生菌Bacillus subtilis LD-8547的诱变选育 [J]. | 江西农业大学学报 , 2012 , 34 (06) : 1251-1255 . |
| MLA | 袁军 et al. "豆豉溶栓酶产生菌Bacillus subtilis LD-8547的诱变选育" . | 江西农业大学学报 34 . 06 (2012) : 1251-1255 . |
| APA | 袁军 , 李国良 , 沈榕强 , 庄振宏 , 杨燕凌 . 豆豉溶栓酶产生菌Bacillus subtilis LD-8547的诱变选育 . | 江西农业大学学报 , 2012 , 34 (06) , 1251-1255 . |
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