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学者姓名：黄天培
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Abstract ：
Enhancing the field persistence of Bacillus thuringiensis (Bt) biopesticides is crucial for efficient pest management. This study presents the first systematic investigation into the mechanisms by which all 20 L-amino acids influence Bt biofilm formation, ultraviolet (UV) resistance, and insecticidal activity. The results demonstrate that amino acids functioned as interfacial modulators: non-polar hydrophobic types (e.g., L-leucine) significantly increased biofilm biomass in wettable powder through hydrophobic adhesion, whereas basic types (e.g., Larginine, L-lysine) enhanced the stability of aqueous suspension concentrate via electrostatic stabilization. Through statistical optimization (Plackett-Burman design and response surface methodology), a synergistic formulation composed of L-arginine, L-lysine, and L-leucine was identified. This formulation increased the biofilm yield of the commercial Kernel 32,000 IU/mg wettable powder by 3.8-fold and improved survival rate by approximately 50 % after 4 h of UV irradiation. At the physio-toxicological level, this amino acid combination reduced the LC50 against second-instar Plutella xylostella larvae by 8.12-fold. Transmission electron microscopy revealed complete microvilli shedding and mitochondrial cristae breakage in the treated midgut, indicating that the synergistic mechanism stems from the disruption of the midgut epithelial barrier. This study provides the first direct link between amino-acid-mediated interfacial interactions and midgut pathological damage, offering molecular-physiological evidence for next-generation, low-dose, highly stable Bt formulation with manageable resistance risks.
Keyword ：
Amino acid synergism Amino acid synergism Bacillus thuringiensis (Bt) Bacillus thuringiensis (Bt) Biofilm formation Biofilm formation Biopesticide optimization Biopesticide optimization Midgut pathophysiology Midgut pathophysiology UV resistance UV resistance
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| GB/T 7714 | Wang, Zixuan , Zhang, Yimeng , Chen, Junjun et al. L-amino acids modulate biofilm formation, UV resistance, and insecticidal activity in Bacillus thuringiensis formulations: Mechanisms and optimization [J]. | PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY , 2026 , 216 . |
| MLA | Wang, Zixuan et al. "L-amino acids modulate biofilm formation, UV resistance, and insecticidal activity in Bacillus thuringiensis formulations: Mechanisms and optimization" . | PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY 216 (2026) . |
| APA | Wang, Zixuan , Zhang, Yimeng , Chen, Junjun , Liu, Qingqing , Luo, Xueqin , Xu, Qiuting et al. L-amino acids modulate biofilm formation, UV resistance, and insecticidal activity in Bacillus thuringiensis formulations: Mechanisms and optimization . | PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY , 2026 , 216 . |
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Exopolysaccharides (EPS) produced by Bacillus species display various biological activities and characteristics such as anti-oxidant, immunomodulatory, anti-bacterial, and bioadhesive effects. These attributes confer Bacillus species broad potential applications in diverse fields such as food, medicine, environment, and agriculture. Moreover, Bacillus-derived EPS are easier to produce and yield higher quantities than plant-derived polysaccharides. Despite these advantages, Bacillus-derived EPS still encounter numerous obstacles in industrial production and commercial applications, including elevated costs, the absence of mature fermentation tank production procedures, and the lack of systematic in vivo and in vitro activity and metabolic evaluation. Therefore, it is essential to gain insight into the current status of structure, production, and applications of Bacillus-derived EPS for facilitating their future broader application. This paper provides a comprehensive overview of the current research on the production, separation, characteristics and applications of these related biological products. Furthermore, this paper summarizes the current challenges impeding industrial production of Bacillus-derived EPS, along with potential solutions, and their prospective applications in enhancing the attributes of beneficial biofilms, laying a solid scientific foundation for the applications of Bacillus-derived EPS in industry and agriculture.
Keyword ：
Applications Applications Bacillus Bacillus Beneficial biofilms Beneficial biofilms Characteristics Characteristics Exopolysaccharides Exopolysaccharides Production Production
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| GB/T 7714 | Yang, Xiaolong , Mao, Yufei , Chen, Lan et al. Structural characteristics, biotechnological production and applications of exopolysaccharides from Bacillus sp.: A comprehensive review [J]. | CARBOHYDRATE POLYMERS , 2025 , 355 . |
| MLA | Yang, Xiaolong et al. "Structural characteristics, biotechnological production and applications of exopolysaccharides from Bacillus sp.: A comprehensive review" . | CARBOHYDRATE POLYMERS 355 (2025) . |
| APA | Yang, Xiaolong , Mao, Yufei , Chen, Lan , Guan, Xiong , Wang, Zixuan , Huang, Tianpei . Structural characteristics, biotechnological production and applications of exopolysaccharides from Bacillus sp.: A comprehensive review . | CARBOHYDRATE POLYMERS , 2025 , 355 . |
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本发明涉及一种利用有机酸提升苏云金芽胞杆菌制剂生物活性的方法。所述方法具体为：向苏云金芽胞杆菌制剂中添加有机酸，从而提升苏云金芽胞杆菌制剂的生物活性；所述苏云金芽胞杆菌制剂为苏云金芽胞杆菌可湿性粉剂；所述有机酸选自棕榈酸、硬脂酸、花生酸、乙酰水杨酸、油酸中的任意一种或多种；所述生物活性包括生物被膜形成能力、抗紫外线能力、杀虫活性中的任意一种或多种。通过本发明的方法，能够显著提升苏云金芽胞杆菌制剂在农业中的应用效果，具有较高的实际应用价值。
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| GB/T 7714 | 黄天培 , 赵梵 , 毛雨飞 et al. 一种利用有机酸提升苏云金芽胞杆菌制剂生物活性的方法 : CN202411726650.4[P]. | 2024-11-28 . |
| MLA | 黄天培 et al. "一种利用有机酸提升苏云金芽胞杆菌制剂生物活性的方法" : CN202411726650.4. | 2024-11-28 . |
| APA | 黄天培 , 赵梵 , 毛雨飞 , 王子瑄 , 苏泓昕 , 梅智峰 et al. 一种利用有机酸提升苏云金芽胞杆菌制剂生物活性的方法 : CN202411726650.4. | 2024-11-28 . |
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Phyllotreta striolata is a major pest of Brassica chinensis, with chemical pesticides being the primary method for pest control. However, their negative impact on crop growth and environmental risks have prompted the search for low-toxicity, environmentally friendly alternatives. This study systematically evaluates the control effectiveness of two commonly used chemical pesticides, Zu Jia (R) and Jie Tiao (R), along with their impact on the growth characteristics of B. chinensis and compares them to the biopesticide Bacillus thuringiensis (Bt) strain G033A. The control efficacy was assessed by measuring the insect population reduction rate and plant physiological indicators (weight, plant height, chlorophyll content, and root length). The differences between biopesticide and the two chemical pesticide treatments were analyzed. The results indicated that both Bt strain G033A and the chemical pesticides significantly controlled P. striolata. However, chemical pesticides (Zu Jia (R) and Jie Tiao (R)) significantly reduced the chlorophyll content, plant height, and weight of B. chinensis (p < 0.05), whereas Bt strain G033A had no significant adverse effects on these plant traits (p > 0.05). The colonization characteristics of the Bt strain suggest potential for sustained pest control, while chemical pesticides exhibited rapid pest suppression effects. The findings highlight a novel IPM strategy that integrates the long-lasting efficacy of Bt biopesticides with the rapid action of chemical pesticides, offering a more sustainable and balanced approach to P. striolata management in B. chinensis cultivation.
Keyword ：
biopesticides biopesticides Brassica chinensis Brassica chinensis chemical pesticides chemical pesticides integrated pest management integrated pest management Phyllotreta striolata Phyllotreta striolata
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| GB/T 7714 | Lin, Fuyong , Muhammad, Musa Hassan , Mao, Yufei et al. Comparative Control of Phyllotreta striolata: Growth-Inhibiting Effects of Chemical Insecticides Versus the Green Advantages of a Biopesticide [J]. | INSECTS , 2025 , 16 (6) . |
| MLA | Lin, Fuyong et al. "Comparative Control of Phyllotreta striolata: Growth-Inhibiting Effects of Chemical Insecticides Versus the Green Advantages of a Biopesticide" . | INSECTS 16 . 6 (2025) . |
| APA | Lin, Fuyong , Muhammad, Musa Hassan , Mao, Yufei , Zhao, Fan , Wang, Zixuan , Hong, Yongcong et al. Comparative Control of Phyllotreta striolata: Growth-Inhibiting Effects of Chemical Insecticides Versus the Green Advantages of a Biopesticide . | INSECTS , 2025 , 16 (6) . |
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This study investigated the effects of fertilizers on the biofilm formation, ultraviolet (UV) resistance, and insecticidal activity of Bacillus thuringiensis (Bt). Bacillus thuringiensis, a widely used microbial pesticide, has a minimal environmental impact and is highly effective against specific pests but is susceptible to environmental factors in field applications. Bacterial biofilms provide protection for Bt, enhancing its survival and functionality in the environment. However, the mechanisms by which fertilizers regulate the characteristics of microbial pesticides and enhance biofilm formation are not well understood. This study evaluated the effects of six fertilizers on the bacterial biofilm formation, the UV resistance, and the insecticidal activities of Bt wettable powders. The results demonstrated that fertilizers significantly enhanced the performance of three Bt preparations (Lv'an, Kang'xin, and Lu'kang). A compound fertilizer with 8.346 g/L of KCl, 2.751 g/L of ZnSO47H2O, and 25.681 mu L/mL of humic acid was identified by response surface optimization, achieving the maximum BBF formation with OD595 value of 2.738. Furthermore, KH2PO4, HA, and ZnSO47H2O notably improved the survivability of Bt preparations under prolonged UV exposure, with the compound fertilizer providing the greatest protection. What's more, fertilizers reduced the LC50 values of all Bt preparations, with the compound fertilizer decreasing the LC50 of the Lv'an Bt wettable powder to 0.139 g/L, a 3.12-fold increase in efficacy. This study demonstrated that fertilizers significantly enhance the UV resistance and insecticidal activity of Bt wettable powders by promoting bacterial biofilm formation. Herein, this study provides new strategies and theoretical support for Bt applications in modern sustainable agriculture.
Keyword ：
Bacillus thuringiensis Bacillus thuringiensis bacterial biofilm bacterial biofilm biocontrol biocontrol fertilizers fertilizers insecticidal activity insecticidal activity wettable powders wettable powders
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| GB/T 7714 | Zhao, Fan , Mao, Yufei , Yang, Jiahong et al. Enhancing Bacillus thuringiensis Performance: Fertilizer-Driven Improvements in Biofilm Formation, UV Protection, and Pest Control Efficacy [J]. | MICROORGANISMS , 2025 , 13 (3) . |
| MLA | Zhao, Fan et al. "Enhancing Bacillus thuringiensis Performance: Fertilizer-Driven Improvements in Biofilm Formation, UV Protection, and Pest Control Efficacy" . | MICROORGANISMS 13 . 3 (2025) . |
| APA | Zhao, Fan , Mao, Yufei , Yang, Jiahong , Yang, Sheng , Guan, Xiong , Wang, Zixuan et al. Enhancing Bacillus thuringiensis Performance: Fertilizer-Driven Improvements in Biofilm Formation, UV Protection, and Pest Control Efficacy . | MICROORGANISMS , 2025 , 13 (3) . |
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Over 200 weed species-predominantly from the Poaceae and Asteraceae families-infest tea plantations. These weeds compete aggressively with tea plants for essential resources (light, water, and nutrients), serve as reservoirs for pathogens and pests, and bioaccumulate phytotoxic compounds (e.g., pyrrolizidine alkaloids and heavy metals), jeopardizing agroecosystem health. Although chemical herbicides are currently the most effective and economical method, their long-term overuse poses ecological risks and threatens agricultural sustainability, highlighting an urgent need for sustainable alternatives. Although chemical herbicides remain the most effective and economical method, their long-term overuse causes ecological risks and challenges agricultural sustainability. This review synthesizes current knowledge on sustainable weed management in tea plantations by evaluating the strengths and limitations of chemical and microbial herbicides. It further advances the novel concepts of chemical-microbial synergy and smart application triangulation-integrated strategies not explicitly addressed in previous reviews-that provide new directions for developing next-generation sustainable weed control. This review systematically examines three critical aspects of weed management in tea plantations: (1) characterizing weed biodiversity and assessing multidimensional threats through taxonomic profiling of invasive species, with particular focus on their cascading impacts on tea agroecosystems including resource competition, disease transmission, and soil toxicity; (2) evaluating the mechanisms and trade-offs of current herbicide approaches through comparative analysis of chemical herbicides (noting their efficiency but ecological concerns) and microbial herbicides (considering their environmental benefits but field performance variability); (3) exploring sustainable optimization strategies by investigating innovative approaches such as precision herbicide formulations (nano-formulations, smart delivery systems) and integrated weed management solutions (microbiome engineering, epigenome editing).
Keyword ：
Chemical herbicides Chemical herbicides Herbicide performance Herbicide performance Microbial herbicides Microbial herbicides Multidimensional hazards Multidimensional hazards Sustainable weed management Sustainable weed management Tea plantation weeds Tea plantation weeds
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| GB/T 7714 | Chen Lan , Yang Xiaolong , Su Zhongzeng et al. Sustainable tea plantations: Harnessing chemical-microbial synergy and smart application triangulation for targeted weed control. [J]. | Journal of advanced research , 2025 . |
| MLA | Chen Lan et al. "Sustainable tea plantations: Harnessing chemical-microbial synergy and smart application triangulation for targeted weed control." . | Journal of advanced research (2025) . |
| APA | Chen Lan , Yang Xiaolong , Su Zhongzeng , Guan Xiong , Wang Zixuan , Huang Tianpei . Sustainable tea plantations: Harnessing chemical-microbial synergy and smart application triangulation for targeted weed control. . | Journal of advanced research , 2025 . |
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In this study, a green and highly efficient method was proposed to synthesize nano-silver chloride (nano-AgCl) using spent mushroom substrate (SMS) extract as a cheap reactant. Nanoparticles were characterized by a series of techniques like x-ray diffraction (XRD), energy dispersive spectroscopy (EDS), scanning electron microscopy (SEM), and transmission electron microscopy (TEM), which showed the formation of near-spherical silver chloride nanoparticles with an average size of about 8.30 nm. Notably, the synthesized nano-silver chloride has a more prominent antibacterial effect against Ralstonia solanacearum (EC50 = 5.18 mg L−1) than non-nano-sized silver chloride particles, nano-silver chloride synthesized by chemical method, and commercial pesticides. In-depth, the study of the mechanism revealed that nano-silver chloride could cause cell membrane disruption, DNA damage and intracellular generation of reactive oxygen species (·OH, ·O2− and 1O2), leading to peroxidation damage in Ralstonia solanacearum (R. solanacearum). Moreover, the reaction between nano-silver chloride and bacteria could be driven by intermolecular forces instead of electrostatic interactions. Our study provides a new approach to synthesizing nano-silver chloride as a highly efficient antibacterial agent and broadens the utilization of agricultural waste spent mushroom substrate. © 2024 The Author(s). Published by IOP Publishing Ltd.
Keyword ：
Biochemistry Biochemistry Chemical bonds Chemical bonds Chlorine compounds Chlorine compounds Cytology Cytology Energy dispersive spectroscopy Energy dispersive spectroscopy High resolution transmission electron microscopy High resolution transmission electron microscopy Scanning electron microscopy Scanning electron microscopy Silver nanoparticles Silver nanoparticles Synthesis (chemical) Synthesis (chemical)
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| GB/T 7714 | Pan, Xiaohong , Xu, Lei , Guan, Xiong et al. Biosynthesis of high antibacterial silver chloride nanoparticles against Ralstonia solanacearum using spent mushroom substrate extract [J]. | Nano Express , 2024 , 5 (1) . |
| MLA | Pan, Xiaohong et al. "Biosynthesis of high antibacterial silver chloride nanoparticles against Ralstonia solanacearum using spent mushroom substrate extract" . | Nano Express 5 . 1 (2024) . |
| APA | Pan, Xiaohong , Xu, Lei , Guan, Xiong , Huang, Tianpei , Hong, Ciqing , Gui, Fangze et al. Biosynthesis of high antibacterial silver chloride nanoparticles against Ralstonia solanacearum using spent mushroom substrate extract . | Nano Express , 2024 , 5 (1) . |
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Bacillus biocontrol agent(s) BCA(s) such as Bacillus cereus, Bacillus thuringiensis and Bacillus subtilis have been widely applied to control insects' pests of plants and pathogenic microbes, improve plant growth, and facilitate their resistance to environmental stresses. In the last decade, researchers have shown that, the application of Bacillus biocontrol agent(s) BCA(s) optimized agricultural production yield, and reduced disease risks in some crops. However, these bacteria encountered various abiotic stresses, among which ultraviolet (UV) radiation severely decrease their efficiency. Researchers have identified several strategies by which Bacillus biocontrol agents resist the negative effects of UV radiation, including transcriptional response, UV mutagenesis, biochemical and artificial means (addition of protective agents). These strategies are governed by distinct pathways, triggered by UV radiation. Herein, the impact of UV radiation on Bacillus biocontrol agent(s) BCA(s) and their mechanisms of resistance were discussed.
Keyword ：
Bacillus Bacillus Biocontrol Biocontrol Resistance mechanism Resistance mechanism UV radiation UV radiation
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| GB/T 7714 | Idris, Aisha Lawan , Li, Wenting , Huang, Fugui et al. Impacts of UV radiation on Bacillus biocontrol agents and their resistance mechanisms [J]. | WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY , 2024 , 40 (2) . |
| MLA | Idris, Aisha Lawan et al. "Impacts of UV radiation on Bacillus biocontrol agents and their resistance mechanisms" . | WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY 40 . 2 (2024) . |
| APA | Idris, Aisha Lawan , Li, Wenting , Huang, Fugui , Lin, Fuyong , Guan, Xiong , Huang, Tianpei . Impacts of UV radiation on Bacillus biocontrol agents and their resistance mechanisms . | WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY , 2024 , 40 (2) . |
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Ultraviolet radiation (UV) is a major abiotic stress resulting in relative short duration of Bacillus thuringiensis (Bt) biopesticides in the field, which is expected to be solved by formation of Bt biofilm with higher UV resistance. Therefore, one of the important prerequisite works is to clarify the functions of biofilm-associated genes on biofilm formation and UV resistance of Bt. In this study, comparative genomics and bioinformatic analysis indicated that BTXL6_19475 gene involved in biofilm formation of Bt XL6 was likely to encode a galactose-1-phosphate uridylyltransferase (GalT, E.C. 2.7.7.12). Heterologous expression of the BTXL6_19475 gene in Escherichia coli and detection of its GalT enzyme activity in vitro proved that the gene did encode GalT. Comparing the wild type Bt strain XL6 with galT gene knockout mutant Bt XL6 Delta galT and its complementary strain Bt XL6 Delta galT::19,475, GalT promoted the biofilm formation and enhanced the UV-B resistance of Bt XL6 likely by increasing its D-ribose production and reducing its alanine aryldamidase activity. GalT did not affect the growth and the cell motility of Bt XL6. A regulation map had been proposed to elucidate how GalT promoted biofilm formation and enhanced UV-B resistance of Bt XL6 by the cross-talk between Leloir pathway, Embden-Meyerhof glycolysis pathway and pentose phosphate pathway. Our finding provides a theoretical basis for the efficient use of biofilm genes to improve the UV resistance of Bt biofilms and thus extend field duration of Bt formulations based on biofilm engineering.
Keyword ：
Bacillus thuringiensis Bacillus thuringiensis Biofilm Biofilm Biopesticide Biopesticide Galactose-1-phosphate uridylyltransferase Galactose-1-phosphate uridylyltransferase GalT GalT UV-B stress UV-B stress
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| GB/T 7714 | Idris, Aisha Lawan , Fan, Xiao , Li, Wen et al. Galactose-1-phosphate uridylyltransferase GalT promotes biofilm formation and enhances UV-B resistance of Bacillus thuringiensis [J]. | WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY , 2024 , 40 (12) . |
| MLA | Idris, Aisha Lawan et al. "Galactose-1-phosphate uridylyltransferase GalT promotes biofilm formation and enhances UV-B resistance of Bacillus thuringiensis" . | WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY 40 . 12 (2024) . |
| APA | Idris, Aisha Lawan , Fan, Xiao , Li, Wen , Pei, Hankun , Muhammad, Musa Hassan , Guan, Xiong et al. Galactose-1-phosphate uridylyltransferase GalT promotes biofilm formation and enhances UV-B resistance of Bacillus thuringiensis . | WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY , 2024 , 40 (12) . |
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本发明公开了一种用于苏云金杆菌可湿性粉剂的增效方法及其采用的增效剂。将苏云金杆菌可湿性粉剂与增效剂混合，以提高苏云金杆菌可湿性粉剂的生物被膜形成能力，提高苏云金杆菌可湿性粉剂的抗紫外线能力，提高苏云金杆菌可湿性粉剂的杀虫药效，提高苏云金杆菌可湿性粉剂在茶树叶片上的定殖能力；其中，增效剂为橙皮精油、农用有机硅、十二烷基苯磺酸钠、吐温80、蓖麻油、茶皂素、印楝素、苦参碱、香芹酚、0.1%藜芦碱根茎提取物可溶液剂、茶氨酸、茶多酚中的一种或多种。本发明通过中肠病理切片观察结果推测，中肠为增效剂作用靶器官。本发明可为新型杀虫剂研发提供借鉴。
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| GB/T 7714 | 黄天培 , 林福永 , 毛雨飞 et al. 一种用于苏云金杆菌可湿性粉剂的增效方法及其采用的增效剂 : CN202410343508.5[P]. | 2024-03-25 . |
| MLA | 黄天培 et al. "一种用于苏云金杆菌可湿性粉剂的增效方法及其采用的增效剂" : CN202410343508.5. | 2024-03-25 . |
| APA | 黄天培 , 林福永 , 毛雨飞 , 赵梵 , 韦爱香 , 刘晴晴 et al. 一种用于苏云金杆菌可湿性粉剂的增效方法及其采用的增效剂 : CN202410343508.5. | 2024-03-25 . |
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