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学者姓名：邓优锦
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Abstract ：
Probiotics exert a favorable regulatory effect on human intestinal health. However, probiotics face challenges in overcoming the highly acidic environment of the stomach and effectively reaching the intestine, which limit their beneficial effects and bioavailability. We developed a pH-responsive probiotic embedding gel carrier, where the internal Tremella polysaccharide (TMP) and Ca2+ were cross-linked to create a porous and isolated water microenvironment for probiotics. The introduction of sodium alginate and TMP not only enhanced the mechanical properties of the gel but also contributed to the formation of an outer layer structure with pH response. The gel system had good gel properties, could be shaped freely, and protected Lactobacillus rhamnosus from stomach acid and bile salts. The in vitro simulation experiments demonstrated that the release rate of carrierprotected probiotics was found to be less than 1.99% in simulated gastric fluid, whereas nearly complete release occurred in simulated intestinal fluid, with a release rate over 95%. The survival rate of probiotics in simulated gastric and intestinal fluids could be enhanced by hydrogel carriers. Simultaneously, the carrier could enhance the resistance of probiotics to bile and antibiotic. The results showed that Alg-Ca2+-4 parts per thousand TMP gel carrier was an ideal material for probiotic delivery.
Keyword ：
Gel carrier Gel carrier Probiotic protection Probiotic protection Release Release Tremella polysaccharides Tremella polysaccharides
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| GB/T 7714 | Chu, Lulu , Deng, Youjin , Zhang, Min et al. The characteristics of sodium alginate-tremella polysaccharide assembled hydrogel induced by calcium ion and its protective effect on Lactobacillus rhamnosus [J]. | FOOD HYDROCOLLOIDS , 2025 , 160 . |
| MLA | Chu, Lulu et al. "The characteristics of sodium alginate-tremella polysaccharide assembled hydrogel induced by calcium ion and its protective effect on Lactobacillus rhamnosus" . | FOOD HYDROCOLLOIDS 160 (2025) . |
| APA | Chu, Lulu , Deng, Youjin , Zhang, Min , Chen, Jieting , Lian, Yiyang , Chen, Bingzhi et al. The characteristics of sodium alginate-tremella polysaccharide assembled hydrogel induced by calcium ion and its protective effect on Lactobacillus rhamnosus . | FOOD HYDROCOLLOIDS , 2025 , 160 . |
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灵芝Ganodorma lingzhi的优良品种灵芝13具有农艺性状较好、子实体产量较高、孢子粉产量较高的优良性状。本研究首先以其单核菌株灵芝13-5为材料，通过三代HiFi测序及Hi-C测序组装成完整的基因组；然后选取5个重复的双核菌株通过Illumina高通量测序后，以灵芝13-5基因组作为参考进行SNP calling，建立了基于灵芝13全基因组的分子标记。基因组包含13条染色体，共45.73 Mb。5个重复菌株SNP calling去重合并后，获得319 074个共有的异核等位差异位点，作为用于灵芝13菌株鉴别的分子标记库。从实验室菌种库中随机抽取7个灵芝菌株，与灵芝13菌株共有的等位差异位点占比介于22.20%–45.18%之间，远低于阈值，说明这些菌株与灵芝13差异较大，不是相同菌株。本研究建立了基于灵芝13全基因组及其异核等位差异位点的分子标记，能够准确区分灵芝13这一优良品种。
Keyword ：
SNP calling SNP calling 三代HiFi测序 三代HiFi测序 品种保护 品种保护 品种鉴别 品种鉴别
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| GB/T 7714 | 刘逸婷 , 蒋晓涵 , 杨春艳 et al. 灵芝优良品种全基因组分子标记的建立 [J]. | 菌物学报 , 2025 , 44 (03) : 40-52 . |
| MLA | 刘逸婷 et al. "灵芝优良品种全基因组分子标记的建立" . | 菌物学报 44 . 03 (2025) : 40-52 . |
| APA | 刘逸婷 , 蒋晓涵 , 杨春艳 , 陈健辉 , 王车昭 , 吕晓萌 et al. 灵芝优良品种全基因组分子标记的建立 . | 菌物学报 , 2025 , 44 (03) , 40-52 . |
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本发明公开了一种杏鲍菇菌种Ple0400及其应用。本发明中，菌种根据基因组测序数据，Pl.e0400是针对袋式栽培选育。以‘日引1号’为亲本，自交选育的；采用Kmer差减组装技术对‘Pl.e0400’和亲本‘日引1号’全基因组测序数据进行两两互比，获得特异性差异片段序列(SV)，获得差异片段(SV)，随机选取4个SV序列设计特异性引物8对，对杏鲍基‘Pl.e0400’和亲本‘日引1号’进行扩增，Chr1A，Chr2A，Chr4A和Chr5A为日引1号与Pl.e0400共有，Chr1，Chr2，Chr4和Chr5为日引1号特有片段。从结果可以得出杏鲍菇‘Pl.e0400’亲本‘日引1号’的DNA指纹存在显著差异，确定是不同品种。Pl.e0400’菌盖为凹面型、浅灰色，菌柄短、米白色、硬度大，栽培周期较短，产量高，耐贮藏、耐运输，有很好的开发应用前景。
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| GB/T 7714 | 陈炳智 , 刘新锐 , 邓优锦 et al. 一种杏鲍菇菌种Ple0400及其应用 : CN202411635153.3[P]. | 2024-11-15 . |
| MLA | 陈炳智 et al. "一种杏鲍菇菌种Ple0400及其应用" : CN202411635153.3. | 2024-11-15 . |
| APA | 陈炳智 , 刘新锐 , 邓优锦 , 刘芳 , 江玉姬 , 岑光红 et al. 一种杏鲍菇菌种Ple0400及其应用 : CN202411635153.3. | 2024-11-15 . |
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本发明公开一种松茸香菇，分类命名为松茸香菇11，已于2025年3月24日保藏于中国典型培养物保藏中心，保藏编号CCTCC NO:M 2025577；分子标记鉴定方法包括菌丝的培养与收集、基因组DNA的提取、DNA指纹PCR扩增和PCR产物的电泳检测；本发明通过开展香菇的杂交育种，培育出菇后筛选，获得了继承了两个亲本的温型较高的优点，菌丝耐温性好，活力高，子实体朵形似松茸，不开伞的松茸香菇11，具有很好地开发应用前景。
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| GB/T 7714 | 刘新锐 , 邓优锦 . 一种松茸香菇及其分子标记鉴定方法 : CN202510654967.X[P]. | 2025-05-21 . |
| MLA | 刘新锐 et al. "一种松茸香菇及其分子标记鉴定方法" : CN202510654967.X. | 2025-05-21 . |
| APA | 刘新锐 , 邓优锦 . 一种松茸香菇及其分子标记鉴定方法 : CN202510654967.X. | 2025-05-21 . |
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附属染色体虽然对真菌的正常生长和发育并非必需,但在子囊菌门真菌中的研究已较为深入,而在担子菌门中的报道则相对较少。本研究对来自不同来源的16株银耳(Tremella fuciformis)进行了全基因组测序,获得了27条完整的单倍型基因组序列,用于比较分析。结果显示这些基因组在大小上存在显著差异,变异幅度超过三分之一,主要由附属染色体和核心染色体中的重复序列引起。银耳基因组包含8至10条核心染色体,其多态性主要源于染色体之间的融合或分离。每个基因组含有2至10条附属染色体,共计107条,这些附属染色体的分布模式与系统发育关系及其与Annulohypoxylon stygium的配对特异性密切相关。附属染色体通常较小,富含转座子或重复序列,基因密度较低,但它们之间的同源性明显高于核心染色体。附加染色体表现出快速而多样的结构变异(SV),导致各系统发育分支内共享基因比例低于核心染色体。在银耳两种形态细胞类型的生长和转化过程中,核心和附属染色体均表现出高频的染色体拷贝数变异。大多数附属染色体上的基因与核心染色体上的基因不具同源性,且其编码的蛋白质在NCBI nr数据库中缺乏匹配序列。本研究首次揭示了担子菌门中附属染色体的多样性,并对其起源提供了新的见解,认为这些染色体可能起源于银耳物种分化之前尚未被探索的其他物种。
Keyword ：
基因组多态性 基因组多态性 快速结构变异 快速结构变异 附属染色体 附属染色体
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| GB/T 7714 | 张金香 , 吴小平 , 邓优锦 . 银耳附属染色体的多态性与起源研究：对基因组分化与结构变异的深入解析 [C] //中国菌物学会2025年学术年会 . 2025 . |
| MLA | 张金香 et al. "银耳附属染色体的多态性与起源研究：对基因组分化与结构变异的深入解析" 中国菌物学会2025年学术年会 . (2025) . |
| APA | 张金香 , 吴小平 , 邓优锦 . 银耳附属染色体的多态性与起源研究：对基因组分化与结构变异的深入解析 中国菌物学会2025年学术年会 . (2025) . |
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Fresh-cut taro, renowned for its high nutritional value and convenience, is prone to rapid browning post-cutting, which hinders its storage life. This study focused on the effects of L-ascorbic acid (AA) combined with ultrasound (US) treatment (AS) on the storage quality and transcriptome analysis of fresh-cut slices of Yongding June Red Taro. Compared to the control (CK) group, AS treatment effectively reduced the weight loss rate of taro slices, maintained higher hardness, delayed the increase of browning, and inhibited the accumulation of O-2(-) and H2O2. Furthermore, the AS group showed increased glutathione levels and maintained higher activities of ascorbate peroxidase and glutathione reductase, yet decreased the contents of flavonoids and reducing sugars. Simultaneously, in the AS group, the activities of tyrosinase and lipoxygenase were lowered, thereby preserving the high sensory quality of fresh-cut taro slices. Transcriptome analysis revealed that differentially expressed genes (DEGs) between the AS and CK groups were annotated and categorized into 50 and 20 functional groups based on the Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) databases, respectively. Notably, both groups exhibited significant enrichment in processes related to photosynthesis, protein processing in the endoplasmic reticulum, and isoflavone biosynthesis. Therefore, we concluded that AS treatment could alleviate oxidative stress and maintain storage quality by regulating metabolic pathways. These findings provide insights into the physiological changes occurring in taro immediately after cutting and serve as an essential basis for developing effective storage and preservation techniques.
Keyword ：
Fresh-cut taro Fresh-cut taro L-ascorbic acid L-ascorbic acid Storage quality Storage quality Transcriptome analysis Transcriptome analysis Ultrasonic treatment Ultrasonic treatment
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| GB/T 7714 | Chen, Lin , Chen, Bingzhi , Chu, Lulu et al. The storage quality and transcriptome analysis of fresh-cut taro by L-ascorbic acid combined with ultrasonic treatment [J]. | ULTRASONICS SONOCHEMISTRY , 2025 , 112 . |
| MLA | Chen, Lin et al. "The storage quality and transcriptome analysis of fresh-cut taro by L-ascorbic acid combined with ultrasonic treatment" . | ULTRASONICS SONOCHEMISTRY 112 (2025) . |
| APA | Chen, Lin , Chen, Bingzhi , Chu, Lulu , Chen, Lili , Xie, Luyu , Deng, Youjin et al. The storage quality and transcriptome analysis of fresh-cut taro by L-ascorbic acid combined with ultrasonic treatment . | ULTRASONICS SONOCHEMISTRY , 2025 , 112 . |
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This study investigated the hepatoprotective mechanisms of Volvariella volvacea fruiting body polypeptide (VVFP, 1-3 kDa) against acute alcohol-induced liver injury using multi-omics approaches. Male ICR mice pretreated with VVFP (100-400 mg/kg) showed significantly prolonged alcohol tolerance latency (p < 0.05) and accelerated sobriety recovery compared to controls. Integrated transcriptomics and metabolomics revealed VVFP's dual regulatory effects: (1) transcriptional regulation of 36 endoplasmic reticulum stress genes (e.g., ERP57, Derl) through protein processing pathways (KEGG:04141), and (2) metabolic modulation of 23 hepatic metabolites, particularly phosphatidylcholines and organic acids, via amino acid biosynthesis and glycerophospholipid metabolism. Cross-omics analysis identified eight coregulated genes (Got1, Arg2, Srm, etc.) interacting with key metabolites (4-guanidinobutyric acid, GABA) through linoleic acid metabolism. These findings demonstrate VVFP's therapeutic potential as a functional food ingredient by highlighting its ability to simultaneously target hepatic stress responses and metabolic homeostasis during alcohol detoxification.
Keyword ：
acute alcoholic hepatitis acute alcoholic hepatitis hepatoprotective mechanisms hepatoprotective mechanisms joint analysis joint analysis metabonomics metabonomics straw mushroom straw mushroom transcriptomics transcriptomics
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| GB/T 7714 | Chen, Bingzhi , Chen, Juanqin , Wu, Huihua et al. Volvariella volvacea Polypeptide Mitigates Alcohol-Induced Liver Injury: A Multi-Omics Study [J]. | FOODS , 2025 , 14 (9) . |
| MLA | Chen, Bingzhi et al. "Volvariella volvacea Polypeptide Mitigates Alcohol-Induced Liver Injury: A Multi-Omics Study" . | FOODS 14 . 9 (2025) . |
| APA | Chen, Bingzhi , Chen, Juanqin , Wu, Huihua , Zhang, Fangyi , Chen, Lili , Zhang, Weibin et al. Volvariella volvacea Polypeptide Mitigates Alcohol-Induced Liver Injury: A Multi-Omics Study . | FOODS , 2025 , 14 (9) . |
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本发明公开了一种杏鲍菇菌株及其应用。本发明中，福杏鲍217是针对瓶式栽培选育，杏鲍菇菌种根据基因组测序数据，采用Kmer差减组装技术对‘福杏鲍217’、亲本‘日引1号’全基因组测序数据进行两两互比，获得特异性差异片段序列(SV)，获得差异片段(SV)，随机选取4个SV序列设计特异性引物4对，对杏鲍基‘福杏鲍217’和亲本‘日引1号’进行扩增，本发明的杏鲍菇菌种‘福杏鲍217’针对瓶式栽培选育，以杏鲍菇‘日引1号’为亲本通过自交杂交选育而得，杏鲍菇菌种保藏编号为CCTCC M 20242462，保藏地址为湖北省武汉市武昌区八一路299号。‘福杏鲍217’菌盖为球面型、浅灰色，菌柄颜色为白色，栽培周期较短，产量高，耐贮藏、耐运输，有很好的开发应用前景。
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| GB/T 7714 | 刘新锐 , 陈炳智 , 邓优锦 et al. 一种杏鲍菇菌株及其应用 : CN202411624224.X[P]. | 2024-11-14 . |
| MLA | 刘新锐 et al. "一种杏鲍菇菌株及其应用" : CN202411624224.X. | 2024-11-14 . |
| APA | 刘新锐 , 陈炳智 , 邓优锦 , 江玉姬 , 傅俊生 , 岑光红 et al. 一种杏鲍菇菌株及其应用 : CN202411624224.X. | 2024-11-14 . |
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本发明提供了一种浅色且高产的杏鲍菇菌株，属于农业微生物领域。本发明的杏鲍菇菌株为刺芹侧耳（Pleurotus eryngii）福杏鲍64，已于2024年11月7日保藏于中国典型培养物保藏中心，保藏编号为CCTCC NO：M 20242460，保藏地址为湖北省武汉市武昌区八一路299号。‘福杏鲍64’菌盖凹面型、浅灰色，菌柄颜色洁白，有很好的开发应用前景。
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| GB/T 7714 | 刘新锐 , 陈炳智 , 邓优锦 et al. 一种浅色且高产的杏鲍菇菌株 : CN202411648008.9[P]. | 2024-11-18 . |
| MLA | 刘新锐 et al. "一种浅色且高产的杏鲍菇菌株" : CN202411648008.9. | 2024-11-18 . |
| APA | 刘新锐 , 陈炳智 , 邓优锦 , 刘芳 , 傅俊生 , 江玉姬 et al. 一种浅色且高产的杏鲍菇菌株 : CN202411648008.9. | 2024-11-18 . |
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2-Phenylethanol (2-PE) is an aromatic compound with a rose-like fragrance that is widely used in food and other industries. Yeasts have been implicated in the biosynthesis of 2-PE; however, few studies have reported the involvement of filamentous fungi. In this study, 2-PE was detected in Annulohypoxylon stygium mycelia grown in both potato dextrose broth (PDB) and sawdust medium. Among the 27 A. stygium strains investigated in this study, the strain "Jinjiling" (strain S20) showed the highest production of 2-PE. Under optimal culture conditions, the concentration of 2-PE was 2.33 g/L. Each of the key genes in Saccharomyces cerevisiae shikimate and Ehrlich pathways was found to have homologous genes in A. stygium. Upon the addition of L-phenylalanine to the medium, there was an upregulation of all key genes in the Ehrlich pathway of A. stygium, which was consistent with that of S. cerevisiae. A. stygium as an associated fungus provides nutrition for the growth of Tremella fuciformis and most spent composts of T. fuciformis contain pure A. stygium mycelium. Our study on the high-efficiency biosynthesis of 2-PE in A. stygium offers a sustainable solution by utilizing the spent compost of T. fuciformis and provides an alternative option for the production of natural 2-PE.
Keyword ：
2-phenylethanol 2-phenylethanol Annulohypoxylon stygium Annulohypoxylon stygium Ehrlich pathway Ehrlich pathway L-phenylalanine L-phenylalanine
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| GB/T 7714 | Tong, Qianwen , Yang, Lizhi , Zhang, Jinxiang et al. Comprehensive investigations of 2-phenylethanol production by the filamentous fungus Annulohypoxylon stygium [J]. | APPLIED MICROBIOLOGY AND BIOTECHNOLOGY , 2024 , 108 (1) . |
| MLA | Tong, Qianwen et al. "Comprehensive investigations of 2-phenylethanol production by the filamentous fungus Annulohypoxylon stygium" . | APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 108 . 1 (2024) . |
| APA | Tong, Qianwen , Yang, Lizhi , Zhang, Jinxiang , Zhang, Yue , Jiang, Yuji , Liu, Xinrui et al. Comprehensive investigations of 2-phenylethanol production by the filamentous fungus Annulohypoxylon stygium . | APPLIED MICROBIOLOGY AND BIOTECHNOLOGY , 2024 , 108 (1) . |
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