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学者姓名：孙淑静
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Abstract ：
Tremella fuciformis polysaccharides have diverse bioactivities and beneficial physicochemical properties, enabling broad use in pharmaceuticals and food. This study analyzed intracellular and extracellular polysaccharides (IPs and EPs) from three T. fuciformis spores, assessing their monosaccharide composition and in vitro antioxidant and hypoglycemic effects. Results showed that TWW01-AX and TWW02-TMS had significantly higher biomass and polysaccharide yields than TYH-SD1. IPs were predominantly composed of Glc, exceeding 60 mol%, whereas EPs were primarily composed of Man, accounting for over 64 mol%. Monosaccharide percentages served as key discriminators between IPs and EPs. EPs exhibited stronger DPPH, hydroxyl radical scavenging, CUPRAC, as well as alpha-glucosidase and alpha-amylase inhibition, while IPs showed superior ABTS radical scavenging activities and FRAP. Ridge regression showed key monosaccharide percentage variations notably influence bioactivities. This study provided a theoretical basis for the functional evaluation of high-quality T. fuciformis polysaccharides and the efficient screening of superior strains.
Keyword ：
In vitro bioactivities In vitro bioactivities Monosaccharide composition Monosaccharide composition Ridge regression Ridge regression T. fuciformis spore polysaccharides T. fuciformis spore polysaccharides
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| GB/T 7714 | Chen, Jianqiu , He, Xiaoyu , Zhou, Shuting et al. Correlation analysis of monosaccharide composition and in vitro antioxidant and hypoglycemic activities of Tremella fuciformis spore polysaccharides [J]. | FOOD CHEMISTRY , 2026 , 498 . |
| MLA | Chen, Jianqiu et al. "Correlation analysis of monosaccharide composition and in vitro antioxidant and hypoglycemic activities of Tremella fuciformis spore polysaccharides" . | FOOD CHEMISTRY 498 (2026) . |
| APA | Chen, Jianqiu , He, Xiaoyu , Zhou, Shuting , Chen, Yujie , Kong, Chunyuan , Deng, Yating et al. Correlation analysis of monosaccharide composition and in vitro antioxidant and hypoglycemic activities of Tremella fuciformis spore polysaccharides . | FOOD CHEMISTRY , 2026 , 498 . |
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In producing active probiotic powder, the encapsulant impacts the viability and functionality of dehydrated bacteria through various mechanisms, which may exhibit synergistic or antagonistic effects. This study investigated the effects of supplementing a water-soluble antioxidant to non-fermentable disaccharide lactose or fermentable disaccharide trehalose as encapsulants for producing Lacticaseibacillus rhamnosus GG (LGG) powders via spray drying. LGG powders prepared with pure sugar encapsulants exhibited inactivations of 0.68-1.02 log, whereas incorporating 0.5 wt% antioxidants significantly increased viability loss, with inactivation levels reaching 1.08-1.71 logs for epigallocatechin gallate (EGCg) and 4.03-4.52 logs for ascorbic acid (AA). More intensive inactivation was observed for trehalose-based powders than for lactose-based powders. The powders showed minimal changes in antioxidant capacity, indicating neutralized oxidative stress during spray drying. The particle morphology and the thermal and hygroscopic properties of LGG-encapsulating powders were minorly affected by the antioxidants. However, LGG cells in antioxidant-supplemented powders bore substantial injuries on growth capability, membrane integrity, and cellular shape. Incubating LGG with each antioxidant confirmed their antibacterial effects in sugar solutions, with EGCg being more inhibitory than AA. 1H NMR revealed the electron-donating behavior of EGCg when interacting with LGG, potentially disrupting the bacteria's metabolic system. AA caused a shift in three out of seven LGG signal peaks toward the low field, and it might induce additional acid stress during dehydration. The findings elucidated the multiple pathways that antioxidative agents interact with LAB during dehydration, including altering the intensity of dehydration-associated stresses, modifying the physicochemical properties of particles, and interfering with cellular activities.
Keyword ：
Encapsulation Encapsulation Nuclear magnetic resonance Nuclear magnetic resonance Oxidative stress Oxidative stress Powder property Powder property Probiotic powder Probiotic powder Protectant Protectant
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| GB/T 7714 | Luo, Jingwen , Li, Xiaohong , Chen, Liding et al. Exploring the Effects and Mechanisms of Epigallocatechin Gallate and Ascorbic Acid as Antioxidative Components in Producing Lacticaseibacillus rhamnosus GG Powder via Spray Drying [J]. | FOOD AND BIOPROCESS TECHNOLOGY , 2025 , 18 (6) : 5589-5604 . |
| MLA | Luo, Jingwen et al. "Exploring the Effects and Mechanisms of Epigallocatechin Gallate and Ascorbic Acid as Antioxidative Components in Producing Lacticaseibacillus rhamnosus GG Powder via Spray Drying" . | FOOD AND BIOPROCESS TECHNOLOGY 18 . 6 (2025) : 5589-5604 . |
| APA | Luo, Jingwen , Li, Xiaohong , Chen, Liding , Min, Na , Sun, Shujing , Chen, Xiao Dong et al. Exploring the Effects and Mechanisms of Epigallocatechin Gallate and Ascorbic Acid as Antioxidative Components in Producing Lacticaseibacillus rhamnosus GG Powder via Spray Drying . | FOOD AND BIOPROCESS TECHNOLOGY , 2025 , 18 (6) , 5589-5604 . |
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Hericium erinaceus has been cultivated in China for centuries, known for its notable edible and medicinal properties. This study investigated the diversity and composition of symbiotic bacteria between the wild and cultivated H. erinaceus strains using high-throughput sequencing of the V3-V4 region of bacterial 16S rDNA, and the bacterial function was preliminarily predicted. In addition, the correlation between bacterial community composition and enzyme activities were also analyzed. The results revealed that the dominant taxa at the phylum level were identical for both strains, including Proteobacteria, Firmicutes, Actinobacteria, and Bacteroidetes, but wild strain (WT) exhibited significantly higher alpha-diversity. Key genera in WT include Acinetobacter, Sphingomonas, and Methylobacterium, while cultivated strain (CT) harbored Glutamicibacter, Halomonas, and Citrobacter. PICRUSt 2 functional predictions revealed significant differences in the abundance of amino acid metabolism, carbohydrate metabolism, energy metabolism, and lipid metabolism between the symbiotic bacteria of WT amd CT. WT symbiotic bacteria focused on salt resistance and stress adaptation, while CT bacteria were associated with nitrogen fixation and growth promotion. Enzyme activity was found to influence the bacterial community structure, with hemicellulose, amylase, and cellulase ranked in descending order of impact. This research revealed the role of symbiotic bacteria in promoting mycelial growth and enhancing environmental adaptability.
Keyword ：
Bacterial community composition Bacterial community composition Bacterial diversity Bacterial diversity Hericium erinaceus Hericium erinaceus High throughput sequencing High throughput sequencing Symbiotic bacteria Symbiotic bacteria
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| GB/T 7714 | Li, Jiahuan , Xu, Shiyi , Yang, Ziwei et al. Differences analysis of symbiotic bacterial community composition between wild and cultivated strains from Hericium erinaceus [J]. | SCIENTIA HORTICULTURAE , 2025 , 339 . |
| MLA | Li, Jiahuan et al. "Differences analysis of symbiotic bacterial community composition between wild and cultivated strains from Hericium erinaceus" . | SCIENTIA HORTICULTURAE 339 (2025) . |
| APA | Li, Jiahuan , Xu, Shiyi , Yang, Ziwei , Li, Deng , Zhuang, Shaowei , Chen, Jianqiu et al. Differences analysis of symbiotic bacterial community composition between wild and cultivated strains from Hericium erinaceus . | SCIENTIA HORTICULTURAE , 2025 , 339 . |
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DNA methylation, a cornerstone of epigenetic regulation, governs critical biological processes including transcriptional modulation, genomic imprinting, and transposon suppression through chromatin architecture remodeling. Recent advances have revealed that aberrant methylation patterns-characterized by spatial-temporal dysregulation and stochastic molecular noise-serve as key drivers of diverse pathological conditions, from oncogenesis to neurodegenerative disorders. However, the field faces dual challenges: (1) current understanding remains fragmented due to the inherent spatiotemporal heterogeneity of methylation landscapes across tissues and developmental stages, and (2) mechanistic insights into non-canonical methylation pathways (particularly 6mA) in non-mammalian systems are conspicuously underdeveloped. This review systematically synthesizes the evolutionary-conserved versus species-specific features of 5-methylcytosine (5mC) and N6-methyladenine (6mA) regulatory networks across three biological kingdoms. Through comparative analysis of methylation/demethylation enzymatic cascades (DNMTs/TETs in mammals, CMTs/ROS1 in plants, and DIM-2/DNMTA in fungi), we propose a unified framework for targeting methylation-associated diseases through precision epigenome editing, while identifying critical knowledge gaps in fungal methylome engineering that demand urgent investigation.
Keyword ：
5mC 5mC 6mA 6mA chromatin architecture chromatin architecture DNA methylation DNA methylation epigenetic regulation epigenetic regulation
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| GB/T 7714 | Liu, Yu , Wang, Ying , Bao, Dapeng et al. Cross-Kingdom DNA Methylation Dynamics: Comparative Mechanisms of 5mC/6mA Regulation and Their Implications in Epigenetic Disorders [J]. | BIOLOGY-BASEL , 2025 , 14 (5) . |
| MLA | Liu, Yu et al. "Cross-Kingdom DNA Methylation Dynamics: Comparative Mechanisms of 5mC/6mA Regulation and Their Implications in Epigenetic Disorders" . | BIOLOGY-BASEL 14 . 5 (2025) . |
| APA | Liu, Yu , Wang, Ying , Bao, Dapeng , Chen, Hongyu , Gong, Ming , Sun, Shujing et al. Cross-Kingdom DNA Methylation Dynamics: Comparative Mechanisms of 5mC/6mA Regulation and Their Implications in Epigenetic Disorders . | BIOLOGY-BASEL , 2025 , 14 (5) . |
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Tremella fuciformis Berk., also known as white fungus and snow fungus, is one of the important edible and medicinal mushrooms in China. The quality characteristics and metabolites of different T. fuciformis varieties directly affect the stability of their processed products. In this study, two new varieties of Tremella fuciformis, namely 'TYH-SD1' (yellow) and 'TWH-SD2' (white), which were obtained by the team through single-spore crossbreeding and its control varieties Tr21 (yellow) and Tr01 (white), were used as test materials. The characteristics and nutritional quality of the four varieties of substrates were comparatively analyzed, while metabolomics was employed to investigate the differences in flavor substances. The results demonstrate that TYH-SD1 and TWH-SD2 had a higher rehydration rate and faster rehydration speed compared with the control strains Tr21 and Tr01, with a smaller stem and higher yield. Notably, TWH-SD2 had a 29.06% increase in its rehydration rate and it had higher contents of crude polysaccharide and vitamin D3. The surface of TYH-SD1 ear pieces exhibited a porous structure with a larger pore size and the surface of TWH-SD2 ear pieces displayed a surface characterized by connected gully-like protrusions and fewer indentations, which were significantly different from that of Tr21 and Tr01 ear pieces. The textural analysis shows that TYH-SD1 and TWH-SD2 ear pieces were softer and more elastic, with greater cohesion and recovery, indicating that they had high tensile and deformation recovery ability. Metabolomics analysis revealed that the relative content of aldehydes in the volatile flavor substances TYH-SD1 and TWH-SD2 was high in n-alpha-aldehyde, nonanaldehyde, and n-pentanal. The relative content of alkanes in TYH-SD1 was second only to that of aldehydes, with decane having the highest content, contributing to its more almond aroma, fruity aroma, and fat aroma. TWH-SD2 exhibited the highest concentration of alcohols, accounting for 43.57%, which may result in a clear, mushroom, and lipid odor. The above results will provide theoretical basis for the further production, processing, and application of the new varieties.
Keyword ：
metabolomics metabolomics Tremella fuciformis Berk. Tremella fuciformis Berk. varieties varieties volatile flavor substance volatile flavor substance
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| GB/T 7714 | Chen, Jianqiu , Wei, Lingli , Xie, Jiacheng et al. Quality Characteristics and Metabolomics Analysis of Two New Varieties of Tremella fuciformis [J]. | HORTICULTURAE , 2025 , 11 (3) . |
| MLA | Chen, Jianqiu et al. "Quality Characteristics and Metabolomics Analysis of Two New Varieties of Tremella fuciformis" . | HORTICULTURAE 11 . 3 (2025) . |
| APA | Chen, Jianqiu , Wei, Lingli , Xie, Jiacheng , Zhang, Yuanyuan , Jia, Jiahui , Chen, Liding et al. Quality Characteristics and Metabolomics Analysis of Two New Varieties of Tremella fuciformis . | HORTICULTURAE , 2025 , 11 (3) . |
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Tremella fuciformis is an important edible and medicinal fungi in China, and it can undergo the dimorphic transition between yeast-like conidia and mycelia form, which is particularly crucial in the production and breeding. As a companion fungus, Annulohypoxylon stygium plays a crucial role in the dimorphic transition of T. fuciformis, and previous study found A. stygium extract can promote yeast-like conidia transit to mycelium, but the mechanism is still unclear. This study determined an optimal concentration of the A.stygium extract for promoting the germination and mycelia growth of T. fuciformis. Time-course proteomics was used to characterize the dynamic changes in protein expression during the dimorphic transition within 72 h. A total of 2095 proteins were differentially expressed, which involved in biological processes such as MAPK signaling pathway, starch and sucrose metabolism. Further bioinformatics revealed that MAPK signaling pathway played the most crucial role, among which TfSte7 gene was the hub gene, during yeast-like conidia transit to mycelium. Molecular biological studies demonstrated that the TfSte7 gene can shorten the germination time and promote mycelial growth in T. fuciformis. This study provides an important molecular basis for deeper understanding of the interaction mechanism between A. stygium and the dimorphic of T. fuciformis.
Keyword ：
Annulohypoxylon stygium Annulohypoxylon stygium Dimorphism transition mechanism Dimorphism transition mechanism Proteomics analysis Proteomics analysis Tremella fuciformis Tremella fuciformis
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| GB/T 7714 | Li, Yaxing , Cao, Jixuan , Zhan, Guanping et al. Time-course proteomics analysis and gene function validation of promoting dimorphic transition of Tremella fuciformis by Annulohypoxylon stygium extract [J]. | FOOD BIOSCIENCE , 2025 , 71 . |
| MLA | Li, Yaxing et al. "Time-course proteomics analysis and gene function validation of promoting dimorphic transition of Tremella fuciformis by Annulohypoxylon stygium extract" . | FOOD BIOSCIENCE 71 (2025) . |
| APA | Li, Yaxing , Cao, Jixuan , Zhan, Guanping , Jia, Jiahui , Fan, Jindun , Shen, Zhengyang et al. Time-course proteomics analysis and gene function validation of promoting dimorphic transition of Tremella fuciformis by Annulohypoxylon stygium extract . | FOOD BIOSCIENCE , 2025 , 71 . |
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本实用新型公开了一种食用菌培育霉变喷药装置，主罐体，所述主罐体下方设置有喷药头；转动安装在所述主罐体侧下方的若干个活动杆；所述活动杆包括第一弯折部、第二弯折部、以及一端连接所述第一弯折部下端的连接部，所述连接部另一端与所述第二弯折部固定连接，通过喷药头对正下方的霉变培育袋进行喷药处理。为预防喷药过程中，霉菌孢子受到冲击造成飞散外溢的情况，设置了套膜将主罐体以下部分包覆，形成只有下方开口半封闭的喷药区，而为进一步加强套膜与活动杆的固定效果，故在所述第二弯折部与连接部的侧面设有若干个通孔，所述通孔与套膜内侧缝接固定。
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| GB/T 7714 | 金文松 , 胡开辉 , 孙淑静 et al. 一种食用菌培育霉变喷药装置 : CN202321892039.X[P]. | 2023-07-18 . |
| MLA | 金文松 et al. "一种食用菌培育霉变喷药装置" : CN202321892039.X. | 2023-07-18 . |
| APA | 金文松 , 胡开辉 , 孙淑静 , 李佳欢 . 一种食用菌培育霉变喷药装置 : CN202321892039.X. | 2023-07-18 . |
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本发明提出一种基于机器视觉的液体菌种生物量的软测量方法，包括：离线采样并解析全发酵周期内液体菌种生物量参数及其演变规律；手工测量的辅助变量特征M‑Features建立生物量参数软测量模型M1；采集不同发酵阶段的液体菌种的可见光图像与可见‑近红外I区‑近红外II区多源波谱数据，构建图谱数据集，搭建多任务机器视觉模型V1用于提取基于机器视觉辅助变量特征E‑Features；联合生物量参数软测量模型M1及多任务机器视觉模型V1，建立E‑Features与M‑Features的关联，得到级联机器视觉软测量模型MV；部署所述MV模型于终端嵌入式智能设备，并设计任务执行的交互软件。该方法可在线实时评估液体菌种发酵过程生物量参数演变的趋势，以指导菌种的投产应用。
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| GB/T 7714 | 叶大鹏 , 武礼宾 , 翁海勇 et al. 一种基于机器视觉的液体菌种生物量的软测量方法 : CN202410596991.8[P]. | 2024-05-14 . |
| MLA | 叶大鹏 et al. "一种基于机器视觉的液体菌种生物量的软测量方法" : CN202410596991.8. | 2024-05-14 . |
| APA | 叶大鹏 , 武礼宾 , 翁海勇 , 孙淑静 , 金文松 , 林铃 . 一种基于机器视觉的液体菌种生物量的软测量方法 : CN202410596991.8. | 2024-05-14 . |
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本发明公开了一种海鲜菇酱油制备方法，其步骤包括：S1，选取新鲜海鲜菇为原料，清洗后，按料液比1：4比例加水，置于负压提取罐中在温度区间温度70‑80℃内进行提取，且过滤掉海鲜菇的残留碎屑及杂质，获得海鲜菇提取液；S2，将海鲜菇提取液按照水体体积20‑30％，置于温度区间为50～55℃水中稀释，本发明以海鲜菇粉替代大豆致敏物豆粕，解决了豆制品过敏人群食用酱油会引起过敏反应等问题；通过梯度变温法烘干海鲜菇，同时通过烘干提升海鲜菇风味。结合采用先高温后中温的变温发酵工艺，显著改善了低盐固态酱油产品的风味，提高了其香味、营养价值，既方便了对豆类产品过敏人群的食用，也对其他无过敏人群提供了一种可供选择的风味酱油。
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| GB/T 7714 | 胡开辉 , 李佳欢 , 金文松 et al. 一种海鲜菇酱油制备方法 : CN202311841688.1[P]. | 2023-12-28 . |
| MLA | 胡开辉 et al. "一种海鲜菇酱油制备方法" : CN202311841688.1. | 2023-12-28 . |
| APA | 胡开辉 , 李佳欢 , 金文松 , 孙淑静 , 陈利丁 . 一种海鲜菇酱油制备方法 : CN202311841688.1. | 2023-12-28 . |
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A bioactive Pleurotus eryngii aqueous extract powder (SPAE) was obtained by spray drying and its performance in terms of physicochemical properties, in vitro digestion, inflammatory factors, and modulation of the intestinal microbiota was explored. The results indicated that the SPAE exhibited a more uniform particle size distribution than P. eryngii polysaccharide (PEP). Meanwhile, a typical absorption peak observed at 843 cm(-1) in the SPAE FTIR spectra indicated the existence of alpha-glycosidic bonds. SPAE exhibited higher antioxidant abilities and superior resistance to digestion in vitro. In addition, SPAE supplementation to mice significantly reduced the release of factors that promote inflammation, enhanced the secretion of anti-inflammatory factors, and sustained maximum production of short-chain fatty acids (SCFAs). Additionally, it significantly enhanced the relative abundance of SCFAs-producing Akkermansia and reduced the abundance of Ruminococcus and Clostridiides in intestines of mice. These results show the potential of SPAE as a novel material with prebiotic effects for the food and pharmaceutical industries.
Keyword ：
antioxidant activity antioxidant activity modulating intestinal microbiota modulating intestinal microbiota physicochemical property physicochemical property Pleurotus eryngii aqueous extract Pleurotus eryngii aqueous extract
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| GB/T 7714 | Chen, Jianqiu , Zhou, Mengling , Chen, Liding et al. Evaluation of Physicochemical Properties and Prebiotics Function of a Bioactive Pleurotus eryngii Aqueous Extract Powder Obtained by Spray Drying [J]. | NUTRIENTS , 2024 , 16 (11) . |
| MLA | Chen, Jianqiu et al. "Evaluation of Physicochemical Properties and Prebiotics Function of a Bioactive Pleurotus eryngii Aqueous Extract Powder Obtained by Spray Drying" . | NUTRIENTS 16 . 11 (2024) . |
| APA | Chen, Jianqiu , Zhou, Mengling , Chen, Liding , Yang, Chengfeng , Deng, Yating , Li, Jiahuan et al. Evaluation of Physicochemical Properties and Prebiotics Function of a Bioactive Pleurotus eryngii Aqueous Extract Powder Obtained by Spray Drying . | NUTRIENTS , 2024 , 16 (11) . |
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