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学者姓名：林月绪
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Abstract ：
Protein lysine acetylation (Kac) modification plays impor-tant roles in diverse physiological functions. However, there is little evidence on the role of Kac modification in bacterial antibiotic resistance. Here, we compared the differential expressions of whole-cell proteins and Kac peptides in oxytetracycline sensitive and oxytetracycline resistance (OXYR) strains of Aeromonas hydrophila using quantitative proteomics technologies. We observed a porin family protein Aha1 downregulated in the OXYR strain, which may have an important role in the OXY resistance. Interestingly, seven of eight Kac peptides of Aha1 decreased abundance in OXYR as well. Microbio-logic assays showed that the K57R, K187R, and K197R Aha1 mutants significantly increased antibiotic resistance to OXY and reduced the intracellular OXY accumulation in OXY stress. Moreover, these Aha1 mutants displayed multidrug resistance features to tetracyclines and beta-lac-tam antibiotics. The 3D model prediction showed that the Kac states of K57, K187, and K197 sites located at the extracellular pore vestibule of Aha1 may be involved in the uptake of specific types of antibiotics. Overall, our results indicate a novel antibiotic resistance mechanism medi-ated by Kac modification, which may provide a clue for the development of antibiotic therapy strategies.
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| GB/T 7714 | Zhang, Lishan , Yao, Zujie , Tang, Huamei et al. The Lysine Acetylation Modification in the Porin Aha1 of Aeromonas hydrophila Regulates the Uptake of Multidrug Antibiotics [J]. | MOLECULAR & CELLULAR PROTEOMICS , 2022 , 21 (9) . |
| MLA | Zhang, Lishan et al. "The Lysine Acetylation Modification in the Porin Aha1 of Aeromonas hydrophila Regulates the Uptake of Multidrug Antibiotics" . | MOLECULAR & CELLULAR PROTEOMICS 21 . 9 (2022) . |
| APA | Zhang, Lishan , Yao, Zujie , Tang, Huamei , Song, Qingli , Song, Huanhuan , Yao, Jindong et al. The Lysine Acetylation Modification in the Porin Aha1 of Aeromonas hydrophila Regulates the Uptake of Multidrug Antibiotics . | MOLECULAR & CELLULAR PROTEOMICS , 2022 , 21 (9) . |
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Bacterial quorum sensing (QS) is a well-known behavior for bacterial communication and plays an important role in various bacterial biological functions. Several approaches have been developed to interrupt the QS mechanism in bacterial pathogens. This study developed a novel, high-throughput screening method combined with phage peptide display libraries and a QS biosensor system (PDQBS) to identify quorum-sensing inhibitors (QSIs). We screened 11 hit peptide candidates for QSI activity, which inhibited the C4-AHL mediated quorum-sensing system. Although the synthetic peptides showed moderate inhibitory effects and six candidate peptides ligated with a cell-penetrating peptide (CPP) dramatically inhibited QS signals. The protective efficacies of candidate DNA expression vector ligated with CPP-QSIs were tested against Aeromonas hydrophila infection in the zebrafish model. The obtained result revealed that at least two peptides (CPP21 and CPP23) exhibited an increased protective ability against A. hydrophila infection with RPSs of >50%. Furthermore, the three candidates CPP-QSIs (CPP21, CPP23, and CPP24), also showed high protective abilities with RPSs of >50% against A. hydrophila infection in the mice model. The qPCR analysis further confirmed that these selected CPP-QSIs might stimulate host immunity and inhibit the virulence of A. hydrophila. Finally, the results of dot blot and ELISA assays indicated that these CPP-QSI peptides could bind to the QS receptor protein, AhyR/CviR. Overall, this study provides a promising approach to screening novel QSI candidates for controlling QS-mediated bacterial infections.
Keyword ：
A A hydrophila hydrophila Phage display Phage display Phage display with a QS biosensor system Phage display with a QS biosensor system QSI reporter QSI reporter Quorum sensing inhibitors Quorum sensing inhibitors
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| GB/T 7714 | Zhang, Lishan , Wu, Yao , Yao, Zujie et al. A high-throughput screening approach for bacterial quorum sensing inhibitors (QSIs) against Aeromonas hydrophila infection [J]. | AQUACULTURE , 2022 , 560 . |
| MLA | Zhang, Lishan et al. "A high-throughput screening approach for bacterial quorum sensing inhibitors (QSIs) against Aeromonas hydrophila infection" . | AQUACULTURE 560 (2022) . |
| APA | Zhang, Lishan , Wu, Yao , Yao, Zujie , Wang, Xiaoyun , Chen, Jiazhen , Yang, Wenxiao et al. A high-throughput screening approach for bacterial quorum sensing inhibitors (QSIs) against Aeromonas hydrophila infection . | AQUACULTURE , 2022 , 560 . |
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Abstract ：
对原位转酯化反应(ISTE)合成脂肪酸甲酯(FAME)的研究进展和主要成果进行了综述。主要从ISTE的原理和特点、生物柴油的原位反应制备和油脂中脂肪酸的原位反应分析等方面展开总结和探讨,以期为国内ISTE相关领域的研究提供参考和借鉴。
Keyword ：
原位 原位 生物柴油 生物柴油 脂肪酸 脂肪酸 脂肪酸甲酯 脂肪酸甲酯 转酯化反应 转酯化反应
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| GB/T 7714 | 张桐栋 , 杜焙炎 , 林月绪 et al. 原位转酯化反应合成脂肪酸甲酯的研究进展 [J]. | 粮食与油脂 , 2022 , 35 (01) : 6-9 . |
| MLA | 张桐栋 et al. "原位转酯化反应合成脂肪酸甲酯的研究进展" . | 粮食与油脂 35 . 01 (2022) : 6-9 . |
| APA | 张桐栋 , 杜焙炎 , 林月绪 , 张惠莹 . 原位转酯化反应合成脂肪酸甲酯的研究进展 . | 粮食与油脂 , 2022 , 35 (01) , 6-9 . |
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为提高化学实验的综合性、创新性、探索性,设计了以自制淀粉酶为催化剂催化淀粉水解制备麦芽糖浆的实验。实验结果表明,自制淀粉酶的最佳催化温度为55°C,符合酶催化反应的特征。实验设计突出农林院校的专业特色,促进理论教学和生产实践相结合,有利于培养大学生的科学精神,同时是一次深刻的课程思政教学过程。
Keyword ：
实验教学 实验教学 淀粉 淀粉 酶催化 酶催化 麦芽糖 麦芽糖
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| GB/T 7714 | 黄玉梓 , 江茂生 , 林月绪 . 麦芽糖浆的制备实验设计及教学效果 [J]. | 大学化学 , 2021 , 36 (08) : 81-84 . |
| MLA | 黄玉梓 et al. "麦芽糖浆的制备实验设计及教学效果" . | 大学化学 36 . 08 (2021) : 81-84 . |
| APA | 黄玉梓 , 江茂生 , 林月绪 . 麦芽糖浆的制备实验设计及教学效果 . | 大学化学 , 2021 , 36 (08) , 81-84 . |
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【目的】ZntR是一种金属调控蛋白,可催化锌外排基因的转录激活,防止细胞内二价Zn离子过量,但其对细菌生理功能的影响目前尚不清楚。【方法】本研究构建了嗜水气单胞菌ATCC7966(Aeromonas hydrophila,A.h)的zntR缺失株及补救株,对菌株的生物被膜形成能力、溶血活性、运动能力和响应金属离子胁迫等生理表型进行评估。【结果】敲除zntR基因的菌株对锌和铬离子胁迫敏感、对钴离子胁迫耐受,并且生物被膜形成能力下降、运动能力增强,这些表型在其补救菌株中均能得到恢复。进一步利用DIA定量蛋白质组学技术比较野生株和zntR缺失株的蛋白表达差异,发现ZntR还可能参与双组分系统、细菌的趋化性等代谢通路的调控。【结论】该研究结果可为今后深入探讨zntR转录因子参与细菌生理功能的调控机制提供理论依据。
Keyword ：
同源重组 同源重组 嗜水气单胞菌 嗜水气单胞菌 转录调控因子 转录调控因子
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| GB/T 7714 | 林月绪 , 张丽珊 , 陈加臻 et al. 嗜水气单胞菌zntR调控的生理功能及其机制研究 [J]. | 微生物学报 , 2021 , 61 (09) : 2765-2775 . |
| MLA | 林月绪 et al. "嗜水气单胞菌zntR调控的生理功能及其机制研究" . | 微生物学报 61 . 09 (2021) : 2765-2775 . |
| APA | 林月绪 , 张丽珊 , 陈加臻 , 农添植 , 邢方婷 , 林向民 et al. 嗜水气单胞菌zntR调控的生理功能及其机制研究 . | 微生物学报 , 2021 , 61 (09) , 2765-2775 . |
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本发明公开了一种以动物废弃物为原料同时制备生物柴油和动物饲料的原位转化方法，其使用的动物废弃物不需经过油脂提取和精炼纯化等前处理，即可同时制备生物柴油和动物饲料。具体为：先将清洗后的动物废弃物干燥，然后匀浆或粉碎，再加入助溶剂和硫酸‑甲醇溶液，然后进行原位转酯化反应；完成后静置，将获得的下层相进行过滤，得到的废渣经过清洗、干燥等处理后制得动物饲料；对上层相进行水洗，除去杂质后再经浓缩、干燥等处理即可得生物柴油。本发明不仅可以节约传统生物柴油制备过程中的提油和精炼等成本，制备较高纯度的生物柴油，还可同时生产具有较高粗蛋白含量的动物饲料副产品，为动物源生物柴油的生产开发和成本控制提供了新思路、新技术。
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| GB/T 7714 | 张桐栋 , 缪颖 , 杜焙炎 et al. 一种以动物废弃物为原料同时制备生物柴油和动物饲料的原位转化方法 : CN202010251895.1[P]. | 2020-04-01 . |
| MLA | 张桐栋 et al. "一种以动物废弃物为原料同时制备生物柴油和动物饲料的原位转化方法" : CN202010251895.1. | 2020-04-01 . |
| APA | 张桐栋 , 缪颖 , 杜焙炎 , 刘月亮 , 林月绪 , 张惠莹 . 一种以动物废弃物为原料同时制备生物柴油和动物饲料的原位转化方法 : CN202010251895.1. | 2020-04-01 . |
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Aeromonas hydrophila is an aquatic pathogen that has been of increasing concern in cultivation fisheries because of antibiotics resistance; however, its intrinsic antibiotics resistance mechanism remains elusive. In this study, a label-free-based proteomics method was used to compare differential protein abundances in response to enoxacin (ENX) stress in A. hydrophila. The results showed a total of 96 proteins were altered, with 43 increased and 53 decreased. Subsequent bioinformatics analysis showed that 11 SOS response-related proteins increased and four chemotaxis proteins decreased in response to ENX stress. Further antibiotics susceptibility assay showed that SOS response inhibitors such as baicalein and curcumin significantly enhanced the bactericidal capacity when compounded with ENX but not with chlortetracycline. These findings indicate the importance of SOS response in quinolones resistance. Moreover, the survival rates of five related knock-out strains (Delta uvrA, Delta ahyR, Delta ompW, Delta cheV, and Delta hgpB) were compared in response to different doses of ENX. Finally, the auto-inducer 1 (AI-1) quorum sensing system may negatively regulate ENX resistance in A. hydrophila. Overall, our data provide insight into the quinolones resistance mechanism and indicate that an effective SOS response inhibitor compound with ENX may be a promising strategy for the treatment of quinolones-resistant A. hydrophila. Biological significance: Bacterial antibiotics resistance has become a very serious public health problem, whereas its intrinsic mechanism remains elusive. To investigate the antibiotics resistant characteristics of fish pathogenic Aeromonas hydrophila, we compared the differential protein abundances in response to enoxacin (ENX) stress using proteomics method in this study. Our bioinformatics analysis showed that SOS response-related proteins increased and chemotaxis proteins decreased in response to ENX stress. Further assays showed that SOS response inhibitors such as baicalein and curcumin significantly enhanced the bactericidal capacity when compounded with ENX but not with chlortetracycline. Moreover, the antibiotics susceptibility of five related knock-out strains were valued in different doses of ENX. Overall, our data provide insight into the quinolones resistance mechanism and indicate that SOS response process and quorum sensing may involve in ENX resistance in A. hydrophila.
Keyword ：
Aeromonas hydrophila Aeromonas hydrophila Antibiotics resistance Antibiotics resistance Enoxacin Enoxacin Proteomics Proteomics SOS response SOS response
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| GB/T 7714 | Zhang, Lishan , Li, Wanxin , Sun, Lina et al. Quantitative proteomics reveals the molecular mechanism of Aeromonas hydrophila in enoxacin stress [J]. | JOURNAL OF PROTEOMICS , 2020 , 211 . |
| MLA | Zhang, Lishan et al. "Quantitative proteomics reveals the molecular mechanism of Aeromonas hydrophila in enoxacin stress" . | JOURNAL OF PROTEOMICS 211 (2020) . |
| APA | Zhang, Lishan , Li, Wanxin , Sun, Lina , Wang, Yuqian , Lin, Yuexu , Lin, Xiangmin . Quantitative proteomics reveals the molecular mechanism of Aeromonas hydrophila in enoxacin stress . | JOURNAL OF PROTEOMICS , 2020 , 211 . |
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Aeromonas hydrophilais an aquatic pathogen of freshwater fish. The emergence of widespread antimicrobial-resistance strains of this pathogen has caused increasing rates of fish infections. Our previous research reported thatA. hydrophila yeeY, a LysR-type transcriptional regulator (LTTR), negatively regulated furazolidone (FZ) resistance. Although, it's intrinsic regulatory mechanism is still unclear. In this study, a data-independent acquisition (DIA) quantitative proteomics method was used to compare the differentially expressed proteins (DEPs) between the Delta yeeYand wild-type strain under FZ treatment. When compared to the control, a total of 594 DEPs were identified in Delta yeeY. Among which, 293 and 301 proteins were substantially increased and decreased in abundance, respectively. Bioinformatics analysis showed that several biological pathways such as the secretion system and protein transport were mainly involved in FZ resistance. Subsequently, the antibiotics susceptibility assays of several gene deletion strains identified from the proteomics results showed that YeeY may regulate some important genes such ascysD,AHA_2766,AHA_3195, andAHA_4275, which affects the FZ resistance inA. hydrophila. Furthermore, 34 antimicrobial resistance genes (ARGs) from the bacterial drug resistance gene database (CARD) were found to be directly or indirectly regulated by YeeY. A subsequent assay of several ARGs mutants showed that Delta AHA_3222increased the susceptibility ofA. hydrophilato FZ, while Delta cysNand Delta AHA_3753decreased the susceptibility rate. Finally, the chromatin immunoprecipitation (ChIP) PCR and an electrophoretic mobility shift assay (EMSA) have revealed that the genes such asAHA_3222andAHA_4275were directly and transcriptionally regulated by YeeY. Taken together, our findings demonstrated that YeeY may participate in antimicrobial resistance ofA. hydrophilato FZ, which provides a new target for the development of novel antimicrobial agents in the future.
Keyword ：
Aeromonas hydrophila Aeromonas hydrophila antimicrobial resistance antimicrobial resistance DIA based proteomics DIA based proteomics furazolidone furazolidone YeeY YeeY
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| GB/T 7714 | Fu, Yuying , Zhang, Lishan , Wang, Guibin et al. The LysR-Type Transcriptional Regulator YeeY Plays Important Roles in the Regulatory of Furazolidone Resistance inAeromonas hydrophila [J]. | FRONTIERS IN MICROBIOLOGY , 2020 , 11 . |
| MLA | Fu, Yuying et al. "The LysR-Type Transcriptional Regulator YeeY Plays Important Roles in the Regulatory of Furazolidone Resistance inAeromonas hydrophila" . | FRONTIERS IN MICROBIOLOGY 11 (2020) . |
| APA | Fu, Yuying , Zhang, Lishan , Wang, Guibin , Lin, Yuexu , Ramanathan, Srinivasan , Yang, Guidi et al. The LysR-Type Transcriptional Regulator YeeY Plays Important Roles in the Regulatory of Furazolidone Resistance inAeromonas hydrophila . | FRONTIERS IN MICROBIOLOGY , 2020 , 11 . |
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Preparation of biodiesel using in situ transesterification has been extensively conducted for agricultural, microbial and algal biomass, while few works have been performed using aquatic animal tissue. In this work, fish processing wastes were collected to perform in situ transesterification using grass carp (Ctenopharyngodon idellus) biomass as a representative with which to optimize the reaction conditions. Under the optimum condition, the highest biodiesel purity reached up to 100% for sea bass wastes, which is higher than the 96.5% specified in the EN 14214-2008. The in situ method proposed here has the potential to save significant costs in biodiesel production compared to conventional methods, which usually require high-cost pretreatment of the raw materials. Additionally, the waste residue byproduct produced has a high protein content, and therefore the potential to be used for high-protein feed. This study is expected to inspire new strategies to prepare biodiesel and high-protein feed simultaneously from aquatic animal biomass using the novel in situ transesterification.
Keyword ：
biodiesel biodiesel biomass biomass co-solvent co-solvent fatty acid fatty acid feed feed fish fish in situ in situ transesterification transesterification
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| GB/T 7714 | Zhang, Tongdong , Du, Beiyan , Lin, Yuexu et al. Production of Biodiesel and High-Protein Feed from Fish Processing Wastes Using In Situ Transesterification [J]. | MOLECULES , 2020 , 25 (7) . |
| MLA | Zhang, Tongdong et al. "Production of Biodiesel and High-Protein Feed from Fish Processing Wastes Using In Situ Transesterification" . | MOLECULES 25 . 7 (2020) . |
| APA | Zhang, Tongdong , Du, Beiyan , Lin, Yuexu , Zhang, Min , Liu, Yueliang . Production of Biodiesel and High-Protein Feed from Fish Processing Wastes Using In Situ Transesterification . | MOLECULES , 2020 , 25 (7) . |
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Our previous studies have profiled lysine acetylation and succinylation modifications in Aeromonas hydrophila protein and have found that CobB may be involved in lysine deacylation; however, its effects on bacterial biological function are still unknown. In this study, a data-independent acquisition (DIA)-based proteomics method was used to compare the protein abundance between cob-deleted mutants and wild-type strains. Of the total 2385 identified proteins, 385 were found to have increased abundance, while only 46 showed decreased abundance. Data analysis revealed that many proteins in six metabolic pathways, ribosome, the bacterial secretion system, protein export, RNA degradation, beta-Lactam resistance and oxidative phosphorylation, were affected by the deletion of cobB. Some proteins, such as outer membrane proteins, the two-component regulatory systems and transcriptional factor, were also regulated by cobB. The following phenotype assays confirmed that the Delta cobB mutant produced more biofilm, migrated farther in soft agar, and was more sensitive to oxidative stress than its WT parent. Taken together, the results presented herein provide insights into the behaviors of sirtuin protein CobB in bacteria and demonstrate its important biological functions in A. hydrophila. Biological significance: The sirtuin protein CobB play crucial roles on lysine deacylation, such as desuccinylation and deacetylation in many bacterial species, while the intrinsic behavior of CobB on bacteria remains elusive. The current DIA-based quantitative proteomics analysis showed that the deletion of A. hydrophila cobB significantly affect the intracellular biological processes. Further phenotype assays validated proteomics results. Overall, our data further confirmed the important roles of CobB on the complex protein-protein interaction network regulation in A. hydrophila.
Keyword ：
Aeromonas hydrophila Aeromonas hydrophila DIA DIA LC MS/MS LC MS/MS Proteomics Proteomics Sirtuin CobB Sirtuin CobB
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| GB/T 7714 | Wang, Guibin , Wang, Yuqian , Zhang, Lishan et al. Proteomics analysis reveals the effect of Aeromonas hydrophila sirtuin CobB on biological functions [J]. | JOURNAL OF PROTEOMICS , 2020 , 225 . |
| MLA | Wang, Guibin et al. "Proteomics analysis reveals the effect of Aeromonas hydrophila sirtuin CobB on biological functions" . | JOURNAL OF PROTEOMICS 225 (2020) . |
| APA | Wang, Guibin , Wang, Yuqian , Zhang, Lishan , Cai, Qilan , Lin, Yuexu , Lin, Ling et al. Proteomics analysis reveals the effect of Aeromonas hydrophila sirtuin CobB on biological functions . | JOURNAL OF PROTEOMICS , 2020 , 225 . |
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