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学者姓名：闫大琦
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Abstract ：
基因编码的靶向蛋白降解是一种以遗传信息为基础的降解分子在细胞内表达编码的治疗方法，已经在许多癌症疾病的“不可药用”蛋白的降解中发挥着极为重要的作用。综述了GE-TPD近年来的一些进展及其E3连接酶和目标蛋白结合域的设计和模块选择；并展望了GE-TPD个性化定制的前景和挑战。
Keyword ：
E3泛素连接酶 E3泛素连接酶 GE-TPD GE-TPD 特异性 特异性 结合域 结合域 蛋白降解 蛋白降解
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| GB/T 7714 | 吴雨晴 , 黄惠珍 , 闫大琦 et al. 基因编码的靶向蛋白降解 [J]. | 福建师范大学学报(自然科学版) , 2025 , 41 (01) : 102-108 . |
| MLA | 吴雨晴 et al. "基因编码的靶向蛋白降解" . | 福建师范大学学报(自然科学版) 41 . 01 (2025) : 102-108 . |
| APA | 吴雨晴 , 黄惠珍 , 闫大琦 , 胡君 . 基因编码的靶向蛋白降解 . | 福建师范大学学报(自然科学版) , 2025 , 41 (01) , 102-108 . |
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Abstract ：
本发明公开了一套用于植物的LSR基因重组系统，属于生物技术领域。该重组系统包含Kp03大丝氨酸重组酶及其对应的attP和attB组成，无需额外提供辅因子就能在水稻和拟南芥细胞中实现高效的基因敲入编辑。本发明的基因编辑蛋白Kp03能够在水稻和拟南芥细胞中表现出高效的质粒水平基因敲入能力，最高可在attB靶位点插入27kb的大DNA片段，而且将attB序列截短到15bp仍能进行有效的基因敲入编辑，从而有利于扩展基因编辑的使用范围。此外还可将3.4kb的外源DNA片段成功插入到水稻基因组中，为植物大片段基因敲入提供了新的基因编辑器，具有重要的应用价值。
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| GB/T 7714 | 熊延 , 闫大琦 , 徐通达 et al. 一套用于植物的LSR基因重组系统 : CN202510086748.6[P]. | 2025-01-20 . |
| MLA | 熊延 et al. "一套用于植物的LSR基因重组系统" : CN202510086748.6. | 2025-01-20 . |
| APA | 熊延 , 闫大琦 , 徐通达 , 朱晓玥 , 孟彦彦 , 张楠 et al. 一套用于植物的LSR基因重组系统 : CN202510086748.6. | 2025-01-20 . |
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Abstract ：
The regulation of protein levels in plants is essential for improving agricultural productivity. Recent studies have explored inducible degradation systems in plants, with some showing promising advancements. This study introduces the NRDN degradation tag as a novel tool for regulating protein stability within the nucleus in Arabidopsis thaliana. Unlike traditional gene knockout methods, NRDN offers real-time, dynamic control over protein degradation, enabling precise studies of nuclear-localized proteins. This discovery provides a valuable tool for regulating protein stability in specific cellular compartments, which presents a versatile approach for dissecting complex cellular processes and offers broad applications in functional genomics and cellular research.
Keyword ：
Arabidopsis Arabidopsis Degradation Degradation Nucleus Nucleus Peptides Peptides Tag Tag
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| GB/T 7714 | Shen, Liting , Huang, Huizhen , Yan, Daqi et al. NRDN: A novel nuclear degradation tag for targeted protein regulation in Arabidopsis [J]. | PLANT SCIENCE , 2025 , 356 . |
| MLA | Shen, Liting et al. "NRDN: A novel nuclear degradation tag for targeted protein regulation in Arabidopsis" . | PLANT SCIENCE 356 (2025) . |
| APA | Shen, Liting , Huang, Huizhen , Yan, Daqi , Ye, Yongsheng , Hu, Jun . NRDN: A novel nuclear degradation tag for targeted protein regulation in Arabidopsis . | PLANT SCIENCE , 2025 , 356 . |
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Abstract ：
Targeted insertion of large DNA sequences into plant genomes remains a major challenge in synthetic biology. Here, we evaluate the large serine recombinase Kp03 for site-specific integration of DNA fragments in rice and Arabidopsis. In transient protoplast assays, Kp03 mediates efficient insertion of donor DNA up to 27.3 kilobases (kb), with plasmid integration efficiencies reaching 99.1% for fragments up to 3.4 kb. Truncation experiments reveal that a minimal 15-bp attB sequence is necessary for integration. As a proof of concept, Kp03 successfully incorporates a 3.4-kb donor DNA into the rice genome at a locus containing this minimal attB sequence. Moreover, in rice callus, combining Kp03 with the NM-PE genome editing system to install a 26-bp attB site enables targeted integration of a 3.4-kb donor at the desired genomic locus. These findings establish Kp03 as a versatile tool for plant genome engineering, with broad applications for synthetic biology.
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| GB/T 7714 | Yan, Daqi , Meng, Yanyan , Zhang, Nan et al. Efficient site-specific integration of kilobase-length DNA fragments in plant cells via Kp03 recombinase [J]. | CELL REPORTS , 2025 , 44 (11) . |
| MLA | Yan, Daqi et al. "Efficient site-specific integration of kilobase-length DNA fragments in plant cells via Kp03 recombinase" . | CELL REPORTS 44 . 11 (2025) . |
| APA | Yan, Daqi , Meng, Yanyan , Zhang, Nan , Zhao, Yali , Ning, Conghui , Zhu, Lina et al. Efficient site-specific integration of kilobase-length DNA fragments in plant cells via Kp03 recombinase . | CELL REPORTS , 2025 , 44 (11) . |
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