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学者姓名：贾坤志
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Abstract ：
Conidia are important for the dispersal of Aspergillus flavus, which usually generates aflatoxin B1 (AFB1) and poses a threat to the safety of agricultural food. The development of conidia is usually susceptible to changes in environmental conditions, such as nutritional status and light. However, how the light signal is involved in the conidiation in A. flavus is still unknown. In this study, LreA was identified to respond to blue light and mediate the promotion of conidiation in A. flavus, which is related to the central development pathway. At the same time, blue light inhibited the biosynthesis of AFB1, which was mediated by LreA and attributed to the transcriptional regulation of aflR and aflS expression. Our findings disclosed the function and mechanism of the blue light sensor LreA in regulating conidiation and AFB1 biosynthesis, which is beneficial for the prevention and control of A. flavus and mycotoxins.
Keyword ：
aflatoxin B1 aflatoxin B1 Aspergillus flavus Aspergillus flavus conidiation conidiation LreA LreA
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| GB/T 7714 | Jia, Kunzhi , Jia, Yipu , Zeng, Qianhua et al. Regulation of Conidiation and Aflatoxin B1 Biosynthesis by a Blue Light Sensor LreA in Aspergillus flavus [J]. | JOURNAL OF FUNGI , 2024 , 10 (9) . |
| MLA | Jia, Kunzhi et al. "Regulation of Conidiation and Aflatoxin B1 Biosynthesis by a Blue Light Sensor LreA in Aspergillus flavus" . | JOURNAL OF FUNGI 10 . 9 (2024) . |
| APA | Jia, Kunzhi , Jia, Yipu , Zeng, Qianhua , Yan, Zhaoqi , Wang, Shihua . Regulation of Conidiation and Aflatoxin B1 Biosynthesis by a Blue Light Sensor LreA in Aspergillus flavus . | JOURNAL OF FUNGI , 2024 , 10 (9) . |
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Monovalent cation proton antiporters (CPAs) play crucial roles in ion and pH homeostasis, which is essential for plant development and environmental adaptation, including salt tolerance. Here, 68 CPA genes were identified in soybean, phylogenetically dividing into 11 Na+/H+ exchangers (NHXs), 12 K+ efflux antiporters (KEAs), and 45 cation/H+ exchangers (CHXs). The GmCPA genes are unevenly distributed across the 20 chromosomes and might expand largely due to segmental duplication in soybean. The GmCPA family underwent purifying selection rather than neutral or positive selections. The cis-element analysis and the publicly available transcriptome data indicated that GmCPAs are involved in development and various environmental adaptations, especially for salt tolerance. Based on the RNA-seq data, twelve of the chosen GmCPA genes were confirmed for their differentially expression under salt or osmotic stresses using qRT-PCR. Among them, GmCHX20a was selected due to its high induction under salt stress for the exploration of its biological function on salt responses by ectopic expressing in Arabidopsis. The results suggest that the overexpression of GmCHX20a increases the sensitivity to salt stress by altering the redox system. Overall, this study provides comprehensive insights into the CPA family in soybean and has the potential to supply new candidate genes to develop salt-tolerant soybean varieties.
Keyword ：
cation/proton antiporter cation/proton antiporter GmCHX20a GmCHX20a salt response salt response soybean soybean
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| GB/T 7714 | Jia, Qi , Song, Junliang , Zheng, Chengwen et al. Genome-Wide Analysis of Cation/Proton Antiporter Family in Soybean (Glycine max) and Functional Analysis of GmCHX20a on Salt Response [J]. | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES , 2023 , 24 (23) . |
| MLA | Jia, Qi et al. "Genome-Wide Analysis of Cation/Proton Antiporter Family in Soybean (Glycine max) and Functional Analysis of GmCHX20a on Salt Response" . | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 24 . 23 (2023) . |
| APA | Jia, Qi , Song, Junliang , Zheng, Chengwen , Fu, Jiahui , Qin, Bin , Zhang, Yongqiang et al. Genome-Wide Analysis of Cation/Proton Antiporter Family in Soybean (Glycine max) and Functional Analysis of GmCHX20a on Salt Response . | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES , 2023 , 24 (23) . |
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本发明提供一种检测重金属汞离子的乳胶微球免疫层析试纸，属于重金属快速检测领域。所述乳胶微球免疫层析试纸条包括以下组分：塑料外壳、样品垫、免疫探针结合垫、硝酸纤维膜和吸水垫；所述免疫探针结合垫滴加有抗汞离子单克隆抗体标记的乳胶微球免疫探针；所述抗汞离子单克隆抗体是抗Hg2+‑ITCBE半抗原鼠源杂交瘤细胞株7A1分泌的单克隆抗体，鼠源杂交瘤细胞株7A1，保藏号为CGMCC No.23879。该乳胶微球免疫层析试纸条，其检测阈值为100 ng/mL，检测限为0.78 ng/mL，其特异性强，灵敏度高，重复性良好，性能稳定，操作要求低，非常适用于粮食中快速检测，对监测重金属汞的残留具有重要意义。
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| GB/T 7714 | 凌素美 , 董明科 , 汪世华 et al. 一种检测重金属汞离子的乳胶微球免疫层析试纸条及其应用 : CN202210268689.0[P]. | 2022-03-18 . |
| MLA | 凌素美 et al. "一种检测重金属汞离子的乳胶微球免疫层析试纸条及其应用" : CN202210268689.0. | 2022-03-18 . |
| APA | 凌素美 , 董明科 , 汪世华 , 王荣智 , 贾坤志 , 徐杨 et al. 一种检测重金属汞离子的乳胶微球免疫层析试纸条及其应用 : CN202210268689.0. | 2022-03-18 . |
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本发明提供一种检测重金属汞离子的纳米花免疫层析试纸条，属于重金属快速检测技术领域。所述纳米花免疫层析试纸条包括以下组分：塑料外壳、样品垫、免疫探针结合垫、硝酸纤维膜和吸水垫；所述免疫探针结合垫滴加有抗汞离子单克隆抗体标记的金纳米免疫探针；所述抗汞离子单克隆抗体是抗Hg2+‑ITCBE半抗原鼠源杂交瘤细胞株7A1分泌的单克隆抗体；鼠源杂交瘤细胞株7A1的保藏号为CGMCC No.23879。该纳米花免疫层析试纸条检测重金属半抗原Hg2+‑ITCBE，检测阈值为50 ng/mL，检测限为0.39 ng/mL；其特异性强，灵敏度高，重复性良好，检测快速，对监测重金属汞的残留具有重要意义。
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| GB/T 7714 | 汪世华 , 董明科 , 凌素美 et al. 一种检测重金属汞离子的纳米花免疫层析试纸条及其应用 : CN202210268690.3[P]. | 2022-03-18 . |
| MLA | 汪世华 et al. "一种检测重金属汞离子的纳米花免疫层析试纸条及其应用" : CN202210268690.3. | 2022-03-18 . |
| APA | 汪世华 , 董明科 , 凌素美 , 王荣智 , 贾坤志 , 林晶晶 et al. 一种检测重金属汞离子的纳米花免疫层析试纸条及其应用 : CN202210268690.3. | 2022-03-18 . |
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Lead (Pb) threatens public health due to its toxicity and nonbiodegradable characteristics. It is of significance to develop a sensitive and rapid method for Pb detection. In this study, monoclonal antibodies against Pb were screened with a high affinity constant (K-aff) of 3.56 x 10(9) L/mol. Au nanosphere particles (AuNS) and Au nanoflower particles (AuNF) were synthesized with a diameter of 15 nm and 60 nm, respectively. The specific anti-Pb antibodies were then immobilized on AuNS and AuNF for probe development. At last, AuNS- and AuNF-based strips were successfully assembled for comparative study, which were able to effectively detect environmental Pb in 10 min. The limits of detection (LODs) were determined to be 3.91 ng/ml and 0.2 ng/ml, respectively. Thus the developed method provides a feasible solution for sensitive and rapid detection of Pb on site, which is beneficial to food safety and pollution control.
Keyword ：
AuNF-based strip AuNF-based strip lead (Pb) lead (Pb) monoclonal antibody monoclonal antibody rapid detection rapid detection sensitivity sensitivity
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| GB/T 7714 | Jia, Kunzhi , Lin, Ming , Zhao, Qiang et al. A sensitive and rapid method of lead detection using nanoparticle technology based on monoclonal antibody [J]. | FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY , 2022 , 10 . |
| MLA | Jia, Kunzhi et al. "A sensitive and rapid method of lead detection using nanoparticle technology based on monoclonal antibody" . | FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY 10 (2022) . |
| APA | Jia, Kunzhi , Lin, Ming , Zhao, Qiang , Dong, Mingke , Ling, Sumei , Wang, Shihua . A sensitive and rapid method of lead detection using nanoparticle technology based on monoclonal antibody . | FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY , 2022 , 10 . |
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Aspergillus flavus poses a threat to society economy and public health due to aflatoxin production. aflN is a gene located in the aflatoxin gene cluster, but the function of AflN is undefined in Aspergillus flavus. In this study, aflN is knocked out and overexpressed to study the function of AflN. The results indicated that the loss of AflN leads to the defect of aflatoxin biosynthesis. AflN is also found to play a role in conidiation but not hyphal growth and sclerotia development. Moreover, AlfN is related to the response to environmental oxidative stress and intracellular levels of reactive oxygen species. At last, AflN is involved in the pathogenicity of Aspergillus flavus to host. These results suggested that AflN played important roles in aflatoxin biosynthesis, conidiation and reactive oxygen species generation in Aspergillus flavus, which will be helpful for the understanding of aflN function, and will be beneficial to the prevention and control of Aspergillus flavus and aflatoxins contamination.
Keyword ：
aflatoxin aflatoxin AflN AflN Aspergillus flavus Aspergillus flavus conidiation conidiation
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| GB/T 7714 | Jia, Kunzhi , Yan, Lijuan , Jia, Yipu et al. aflN Is Involved in the Biosynthesis of Aflatoxin and Conidiation in Aspergillus flavus [J]. | TOXINS , 2021 , 13 (11) . |
| MLA | Jia, Kunzhi et al. "aflN Is Involved in the Biosynthesis of Aflatoxin and Conidiation in Aspergillus flavus" . | TOXINS 13 . 11 (2021) . |
| APA | Jia, Kunzhi , Yan, Lijuan , Jia, Yipu , Xu, Shuting , Yan, Zhaoqi , Wang, Shihua . aflN Is Involved in the Biosynthesis of Aflatoxin and Conidiation in Aspergillus flavus . | TOXINS , 2021 , 13 (11) . |
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本发明属于食品安全免疫检测领域，涉及一种基于单克隆抗体检测细交链格孢菌酮酸的金纳米花快速检测试纸，提供的抗细交链格孢菌酮酸单克隆抗体6D5，亲和力常数Kaff达到1.72×1010 L/mol；可识别细交链格孢菌酮酸，对TA的50%抑制浓度IC50达到2.50 ng/mL。本发明检测试纸，检测时间15min，检测阈值(消除T线)达到50ng/mL，视觉检测限(vLOD)达到0.78 ng/mL。可实现细交链格孢菌酮酸的高灵敏快速检测，确保细交链格孢菌酮酸的有效监测与防控。
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| GB/T 7714 | 汪世华 , 蔡培原 , 王荣智 et al. 一种基于单克隆抗体检测细交链格孢菌酮酸的金纳米花快速检测试纸 : CN202011268779.7[P]. | 2020-11-13 . |
| MLA | 汪世华 et al. "一种基于单克隆抗体检测细交链格孢菌酮酸的金纳米花快速检测试纸" : CN202011268779.7. | 2020-11-13 . |
| APA | 汪世华 , 蔡培原 , 王荣智 , 凌素美 , 贾坤志 . 一种基于单克隆抗体检测细交链格孢菌酮酸的金纳米花快速检测试纸 : CN202011268779.7. | 2020-11-13 . |
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本发明属于免疫检测领域，涉及一种检测细交链孢菌酮酸的核壳型金铂合金纳米免疫层析试纸，所述检测细交链格孢菌酮酸的核壳型金铂合金纳米免疫层析试纸检测阈值(消除T线)为25 ng/mL，视觉检测限(vLOD)为0.39 ng/mL；与常规的胶体金试纸相比，检测阈值(消除T线)降低4倍，视觉检测限(vLOD)降低32倍。本发明提供一种细交链格孢菌酮酸的高灵敏快速检测方法。
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| GB/T 7714 | 汪世华 , 蔡培原 , 王荣智 et al. 一种检测细交链孢菌酮酸的核壳型金铂合金纳米免疫层析试纸 : CN202011268776.3[P]. | 2020-11-13 . |
| MLA | 汪世华 et al. "一种检测细交链孢菌酮酸的核壳型金铂合金纳米免疫层析试纸" : CN202011268776.3. | 2020-11-13 . |
| APA | 汪世华 , 蔡培原 , 王荣智 , 凌素美 , 贾坤志 . 一种检测细交链孢菌酮酸的核壳型金铂合金纳米免疫层析试纸 : CN202011268776.3. | 2020-11-13 . |
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本发明属于食品安全免疫检测领域，涉及一种基于单克隆抗体检测重金属铅的金纳米花快速检测试纸。本发明提供的抗重金属铅单克隆抗体9B7，亲和力常数Kaff达到9.56×109 L/mol；对Pb‑iEDTA的50%抑制浓度IC50达到153 ng/mL。本发明提供的一种检测铅离子的金纳米花快速检测试纸，检测时间10min，检测阈值(消除T线)达到100 ng/mL，检测限(vLOD)达到1.562 ng/mL。首次利用纳米花材料与抗重金属铅单克隆抗体结合，制备金纳米花试纸条，具有更高的灵敏度，可实现粮食中铅残留的高灵敏快速检测，确保对重金属铅残留的有效监测与防控。
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| GB/T 7714 | 贾坤志 , 林明 , 汪世华 et al. 一种基于金纳米花技术检测铅残留的检测试纸 : CN202011398272.3[P]. | 2020-12-04 . |
| MLA | 贾坤志 et al. "一种基于金纳米花技术检测铅残留的检测试纸" : CN202011398272.3. | 2020-12-04 . |
| APA | 贾坤志 , 林明 , 汪世华 , 凌素美 , 王荣智 , 黄晓婷 et al. 一种基于金纳米花技术检测铅残留的检测试纸 : CN202011398272.3. | 2020-12-04 . |
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本发明属于抗体工程与免疫检测领域，具体涉及一种牛乳中乳铁蛋白竞争法纳米花免疫测流层析检测卡。该纳米花免疫测流层析检测卡包括样品垫、纳米花金标垫、硝酸纤维素膜(NC膜)、吸水垫和塑料卡壳。所述的NC膜上设置有C线和T线，所述的C线上喷有羊抗鼠的IgG抗体，而所述的T线上包被有牛乳铁蛋白抗原。所述的金标垫上含有纳米花金标记的鼠源单克隆IgG抗体。本发明能够通过竞争法纳米花免疫检测卡，对牛奶中乳铁蛋白进行快速检测，其检测灵敏度为2.4 ng/mL, 对牛奶中主要的酪蛋白、血清白蛋白和脱脂牛奶均无交叉反应，所建立的检测方法方便快捷可以直接用于乳产品中乳铁蛋白含量的快速检测。
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| GB/T 7714 | 王荣智 , 王俊程 , 汪世华 et al. 一种牛乳中乳铁蛋白竞争法纳米花免疫测流层析检测卡 : CN201910429625.2[P]. | 2019-05-22 . |
| MLA | 王荣智 et al. "一种牛乳中乳铁蛋白竞争法纳米花免疫测流层析检测卡" : CN201910429625.2. | 2019-05-22 . |
| APA | 王荣智 , 王俊程 , 汪世华 , 刘海梅 , 李秀兰 , 凌素美 et al. 一种牛乳中乳铁蛋白竞争法纳米花免疫测流层析检测卡 : CN201910429625.2. | 2019-05-22 . |
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