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学者姓名：任育军
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Abstract ：
WRKY transcription factors play a predominant role in plant stress responses, as well as growth and development. Although WRKY genes have been extensively studied in model plants, little is known about them in Brassica rapa. In this study, the BrWRKY22 gene was isolated and characterized. BrWRKY22 is nuclear localized and has self-activation and dimerization activity. BrWRKY22 was highly expressed in young leaves, roots, and stems. The overexpressed BrWRKY22 Arabidopsis and Brassica rapa lines exhibited a dwarfish, delayed flowering and leaf senescence phenotype compared to the wild-type (WT). Molecular evidence showed that the transcript levels of BrCHLP are increased, whereas those of BrLFY, BrSOC1, BrGA20OX2, BrGA3OX1, and BrGASA6 are significantly decreased in BrWRKY22 overexpressing plants compared to the WT. BrWRKY22 can bind directly to the promoters of BrCHLP and BrGA20OX2, activating BrCHLP and repressing BrGA20OX2 gene transcription. The chlorophyll b and tocopherol levels are increased, whereas the GA and ABA levels are significantly decreased, in three-week-old BrWRKY22 overexpressing Brassica lines compared to the WT. Collectively, our results suggest that BrWRKY22 directly controls chlorophyll b and GA biosynthesis and plays a repressive role in leaf senescence and the initiation of flowering in Brassica rapa plant development.
Keyword ：
Brassica Brassica chlorophyll chlorophyll flowering flowering gibberellin gibberellin senescence senescence WRKY transcription factor WRKY transcription factor
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| GB/T 7714 | Uddin, Junaite Bin Gias , Zhuo, Tingzhen , Li, Xiaojie et al. Overexpressing BrWRKY22 Delays Flowering and Leaf Senescence via Inhibition of GA Biosynthesis in Brassica rapa [J]. | PLANTS-BASEL , 2025 , 14 (11) . |
| MLA | Uddin, Junaite Bin Gias et al. "Overexpressing BrWRKY22 Delays Flowering and Leaf Senescence via Inhibition of GA Biosynthesis in Brassica rapa" . | PLANTS-BASEL 14 . 11 (2025) . |
| APA | Uddin, Junaite Bin Gias , Zhuo, Tingzhen , Li, Xiaojie , Wu, Xuan , Wu, Zhuoyu , Ren, Yujun et al. Overexpressing BrWRKY22 Delays Flowering and Leaf Senescence via Inhibition of GA Biosynthesis in Brassica rapa . | PLANTS-BASEL , 2025 , 14 (11) . |
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Plant senescence is an integrated programme of plant development that aims to remobilize nutrients and energy from senescing tissues to developing organs under developmental and stress-induced conditions. Upstream in the regulatory network, a small family of single-stranded DNA/RNA-binding proteins known as WHIRLYs occupy a central node, acting at multiple regulatory levels and via trans-localization between the nucleus and organelles. In this review, we summarize the current progress on the role of WHIRLY members in plant development and stress-induced senescence. WHIRLY proteins can be traced back in evolution to green algae. WHIRLY proteins trade off the balance of plant developmental senescence and stress-induced senescence through maintaining organelle genome stability via R-loop homeostasis, repressing the transcription at a configuration condition, and recruiting RNA to impact organelle RNA editing and splicing, as evidenced in several species. WHIRLY proteins also act as retrograde signal transducers between organelles and the nucleus through protein modification and stromule or vesicle trafficking. In addition, WHIRLY proteins interact with hormones, reactive oxygen species and environmental signals to orchestrate cell fate in an age-dependent manner. Finally, prospects for further research and promotion to improve crop production under environmental constraints are highlighted.
Keyword ：
Evolutionary Evolutionary organelle-nucleus retrograde signals organelle-nucleus retrograde signals plant developmental senescence plant developmental senescence regulatory mechanism regulatory mechanism stress-induced senescence stress-induced senescence WHIRLY family WHIRLY family
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| GB/T 7714 | Lin, Wenfang , Huang, Dongmei , Li, Mengsi et al. WHIRLY proteins, multi-layer regulators linking the nucleus and organelles in developmental and stress-induced senescence of plants [J]. | ANNALS OF BOTANY , 2024 , 134 (4) : 521-536 . |
| MLA | Lin, Wenfang et al. "WHIRLY proteins, multi-layer regulators linking the nucleus and organelles in developmental and stress-induced senescence of plants" . | ANNALS OF BOTANY 134 . 4 (2024) : 521-536 . |
| APA | Lin, Wenfang , Huang, Dongmei , Li, Mengsi , Ren, Yujun , Zheng, Xiangzi , Wu, Binghua et al. WHIRLY proteins, multi-layer regulators linking the nucleus and organelles in developmental and stress-induced senescence of plants . | ANNALS OF BOTANY , 2024 , 134 (4) , 521-536 . |
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The color of flowers is one of the main characteristics adopted for plants to attract pollinators to ensure the reproductive success of the plant, they are also important in their ornamental appeal in Narcissus plant. In this study, we identified a NtMYB12 locus encoding an R2R3-MYB transcription factor. Comparative transcriptome analysis of loss- and gain- of NtMYB12 tissue relative to wild-type narcissus showed NtMYB12 was mainly involved in flavonol and phenylpropanoid metabolic pathways. Biochemical evidences of dual-luciferase activity and chromatin immunoprecipitation assay supported that MYB12 directly bound to promoters of NtFLS, NtLAR, and NtDFR that were cloned by genome walking assay, and activated NtFLS and NtLAR expression but repressed NtDFR expression. More interestingly, NtMYB12 can interact with NtbHLH1 and NtWD40-1 proteins via R3 domain that were selected by transcriptome-based WGCNA and confirmed by yeast two hybrid, bimolecular fluorescence complementation and coimmunoprecipitation assay. Interaction of NtMYB12 with NtbHLH1 and NtWD40-1 forming MYB-bHLH-WD40 triplex specially activated NtDFR and NtANS expression and promoted (pro)anthocyanin accumulation, while NtMYB12 alone activated NtFLS and NtLAR expression and accumulated flavonols, but repressed NtDFR expression. These results indicated that NtMYB12 alone or NtMYB12-bHLH1-WD40-1 triplex requires for competition of metabolism fluxes between flavonol and (pro)anthocyanin biosynthesis.
Keyword ：
Flavonol biosynthesis Flavonol biosynthesis MBW triplex MBW triplex Narcissus tazetta Narcissus tazetta NtMYB12 NtMYB12 Proanthocyanin biosynthesis Proanthocyanin biosynthesis
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| GB/T 7714 | Yang, Jingwen , Wu, Xi , Aucapina, Cristina belen et al. NtMYB12 requires for competition between flavonol and (pro)anthocyanin biosynthesis in Narcissus tazetta tepals [J]. | MOLECULAR HORTICULTURE , 2023 , 3 (1) . |
| MLA | Yang, Jingwen et al. "NtMYB12 requires for competition between flavonol and (pro)anthocyanin biosynthesis in Narcissus tazetta tepals" . | MOLECULAR HORTICULTURE 3 . 1 (2023) . |
| APA | Yang, Jingwen , Wu, Xi , Aucapina, Cristina belen , Zhang, Deyu , Huang, Jiazhi , Hao, Ziyuan et al. NtMYB12 requires for competition between flavonol and (pro)anthocyanin biosynthesis in Narcissus tazetta tepals . | MOLECULAR HORTICULTURE , 2023 , 3 (1) . |
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Xylogen-like arabinogalactan protein (XYLP) is an atypical lipid transport protein. In this study, 23 Phyllostachys edulis XYLPs were identified, and their proteins contain characteristic structures of AGP and nsLTP domain. All PeXYLPs can be divided into four clades, and their genes were unevenly distributed on 11 chromosome scaffolds. Collinear analysis revealed that segmental duplication was the main driver for PeXYLP family expansion. The cis -acting elements presented in the promoter are involved in various regulations of PeXYLPs expression. G.O. annotation revealed that PeXYLPs are mainly interested in lipid transport and synthesis and primarily function at the plasma membrane. Transcriptome analysis revealed that PeXYLPs were spatiotemporally expressed and displayed significant variability during various tissue development. Besides that, some PeXYLPs also respond to multiple phytohormones and abiotic stresses. By semi-quantitative RT-PCR, the response of some PeXYLPs to MeJA was confirmed, and the proteins were shown to localize to the plasma membrane mainly. WGCNA in defined regions of fast-growing bamboo shoots revealed that 5 PeXYLPs in 4 gene co-expression modules showed a positive module-trait relationship with three fast-growing regions. This systematic analysis of the PeXYLP family will provide a foundation for further insight into the functions of individual PeXYLP in a specific tissue or organ development, phytohormone perception, and stress responses in the future.
Keyword ：
Gene family expansion Gene family expansion Internode elongation Internode elongation Weighted gene co -expression network analysis Weighted gene co -expression network analysis XYLP gene family XYLP gene family
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| GB/T 7714 | Rao, Jialin , Huang, Zihong , Chen, Zhongxian et al. Identification and expression profiles of xylogen-like arabinogalactan protein (XYLP) gene family in Phyllostachys edulis in different developmental tissues and under various abiotic stresses [J]. | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES , 2023 , 227 : 1098-1118 . |
| MLA | Rao, Jialin et al. "Identification and expression profiles of xylogen-like arabinogalactan protein (XYLP) gene family in Phyllostachys edulis in different developmental tissues and under various abiotic stresses" . | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 227 (2023) : 1098-1118 . |
| APA | Rao, Jialin , Huang, Zihong , Chen, Zhongxian , Liu, Hongfei , Zhang, Xiaoting , Cen, Xuexiang et al. Identification and expression profiles of xylogen-like arabinogalactan protein (XYLP) gene family in Phyllostachys edulis in different developmental tissues and under various abiotic stresses . | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES , 2023 , 227 , 1098-1118 . |
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Transcription factor NtMYB1 targeting the promoters of NtNCED1/2 and activating their transcription redirects metabolic flux of abscisic acid and & beta;-carotene biosynthesis, regulating petal senescence and leaf yellowing in Narcissus . Chinese narcissus (Narcissus tazetta var. chinensis cv. 'Jingzhanyuntai') is one of the 10 most famous traditional flowers of China, having a beautiful and highly ornamental flower with a rich fragrance. However, the flower longevity affects its commercial appeal. While petal senescence in Narcissus is ethylene-independent and abscisic acid-dependent, the regulatory mechanism has yet to be determined. In this study, we identified a R2R3-MYB gene (NtMYB1) from Narcissus tazetta and generated oeNtMYB1 and Ntmyb1 RNA interference mutants in Narcissus as well as an oeNtMYB1 construct in Arabidopsis. Overexpressing NtMYB1 in Narcissus or Arabidopsis led to premature leaf yellowing, an elevated level of total carotenoid, a reduced level of chlorophyll b, and a decrease in photosystem II fluorescence (Fv/Fm). A dual-luciferase assay and chromatin immunoprecipitation-quantitative PCR revealed that NtMYB1 directly binds to the promoter of NtNCED1 or NtNCED2 and activates NtNCED1/2 gene expression both in vitro and in vivo. Moreover, overexpressing NtMYB1 accelerated abscisic acid biosynthesis, up-regulated the content of zeatin and abscisic acid, and down-regulated the level of & beta;-carotene and gibberellin A1, leading to petal senescence and leaf yellowing in Narcissus. This study revealed a regulatory process that is fundamentally different between non-photosynthetic organs and leaves.
Keyword ：
ABA biosynthesis ABA biosynthesis metabolic flux metabolic flux Narcissus Narcissus NtMYB1 NtMYB1 NtNCED NtNCED tepal senescence tepal senescence
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| GB/T 7714 | Yang, Jingwen , Huang, Jiazhi , Wu, Xi et al. NtMYB1 and NtNCED1/2 control abscisic acid biosynthesis and tepal senescence in Chinese narcissus (Narcissus tazetta) [J]. | JOURNAL OF EXPERIMENTAL BOTANY , 2023 , 74 (21) : 6505-6521 . |
| MLA | Yang, Jingwen et al. "NtMYB1 and NtNCED1/2 control abscisic acid biosynthesis and tepal senescence in Chinese narcissus (Narcissus tazetta)" . | JOURNAL OF EXPERIMENTAL BOTANY 74 . 21 (2023) : 6505-6521 . |
| APA | Yang, Jingwen , Huang, Jiazhi , Wu, Xi , Xu, Yun , Gu, Zheng , Chen, Yajue et al. NtMYB1 and NtNCED1/2 control abscisic acid biosynthesis and tepal senescence in Chinese narcissus (Narcissus tazetta) . | JOURNAL OF EXPERIMENTAL BOTANY , 2023 , 74 (21) , 6505-6521 . |
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The translocation of proteins between various compartments of cells is the simplest and most direct way of an/retrograde communication. However, the mechanism of protein trafficking is far understood. In this study, we showed that the alteration of WHY2 protein abundance in various compartments of cells was dependent on a HECT-type ubiquitin E3 ligase UPL5 interacting with WHY2 in the cytoplasm, plastid, and nucleus, as well as mitochondrion to selectively ubiquitinate various Kub-sites (Kub 45 and Kub 227) of WHY2. Plastid genome stability can be maintained by the UPL5-WHY2 module, accompany by the alter-ation of photosystem activity and senescence-associated gene expression. In addition, the specificity of UPL5 ubiquitinating various Kub-sites of WHY2 was responded to cold or CaCl2 stress, in a dose [Ca2+](cyt)-dependent manner. This demonstrates the integration of the UPL5 ubiquitination with the regulation of WHY2 distribution and retrograde communication between organelle and nuclear events of leaf senescence.
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| GB/T 7714 | Lan, Wei , Ma, Weibo , Zheng, Shuai et al. UPL5 modulates WHY2 protein distribution in a Kub-site dependent ubiquitination in response to [Ca2+]cyt-induced leaf senescence [J]. | ISCIENCE , 2023 , 26 (3) . |
| MLA | Lan, Wei et al. "UPL5 modulates WHY2 protein distribution in a Kub-site dependent ubiquitination in response to [Ca2+]cyt-induced leaf senescence" . | ISCIENCE 26 . 3 (2023) . |
| APA | Lan, Wei , Ma, Weibo , Zheng, Shuai , Yang, Ping , Qiu, Yuhao , Lin, Wenfang et al. UPL5 modulates WHY2 protein distribution in a Kub-site dependent ubiquitination in response to [Ca2+]cyt-induced leaf senescence . | ISCIENCE , 2023 , 26 (3) . |
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本发明公开了一种建立农杆菌介导中国水仙高效遗传转化体系的方法，包括以下步骤：植物材料预处理、外植体消毒、愈伤组织诱导、侵染液制备、农杆菌侵染、共培养和筛选培养以及GUS染色检测。本发明以中国水仙子房为转化材料，通过农杆菌类型和愈伤组织预培养时间及卡那霉素浓度的优化，建立了中国水仙的高效遗传转化体系，且转化效率高，为中国水仙种植资源创制及品种改良奠定了基础。
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| GB/T 7714 | 黄佳志 , 缪颖 , 任育军 . 一种建立农杆菌介导中国水仙高效遗传转化体系的方法 : CN202210162166.8[P]. | 2022-02-22 . |
| MLA | 黄佳志 et al. "一种建立农杆菌介导中国水仙高效遗传转化体系的方法" : CN202210162166.8. | 2022-02-22 . |
| APA | 黄佳志 , 缪颖 , 任育军 . 一种建立农杆菌介导中国水仙高效遗传转化体系的方法 : CN202210162166.8. | 2022-02-22 . |
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Recent studies have confirmed that chlorophyllase (CLH), a long-found chlorophyll (Chl) dephytylation enzyme for initiating Chl catabolism, has no function in leaf senescence-related Chl breakdown. Yet, CLH is considered to be involved in fruit degreening and responds to external and hormonal stimuli. The purpose of this work was to elucidate in detail the biochemical, structural properties, and gene expression of four CLHs from the Solanum lycopersicum genome so as to understand the roles of Solanum lycopersicum chlorophyllases (SlCLHs). SlCLH1/4 were the predominantly expressed CLH genes during leaf and fruit development/ripening stages, and SlCLH1 in mature green fruit was modulated by light. SlCLH1/2/3/4 contained a highly conserved GHSXG lipase motif and a Ser-Asp-His catalytic triad. We identified Ser159, Asp226, and His258 as the essential catalytic triad by site-directed mutagenesis in recombinant SlCLH1. Kinetic analysis of the recombinant enzymes revealed that SlCLH1 had high hydrolysis activities against Chl a, Chl b, and pheophytin a (Phein a), but preferred Chl a and Chl b over Phein a; SlCLH2/3 only showed very low activity to Chl a and Chl b, while SlCLH4 showed no Chl dephytylation activity. The recombinant SlCLH1/2/3 had different pH stability and temperature optimum. Removal of the predicted N-terminal processing peptide caused a partial loss of activity in recombinant SlCLH1/2 but did not compromise SlCLH3 activity. These different characteristics among SlCLHs imply that they may have different physiological functions in tomato.
Keyword ：
biochemical characteristics biochemical characteristics catalytic triad catalytic triad chlorophyllase chlorophyllase substrate specificity substrate specificity
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| GB/T 7714 | Liu, Guangyuan , Meng, Xue , Ren, Yujun et al. Genes, Structural, and Biochemical Characterization of Four Chlorophyllases from Solanum lycopersicum [J]. | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES , 2022 , 23 (19) . |
| MLA | Liu, Guangyuan et al. "Genes, Structural, and Biochemical Characterization of Four Chlorophyllases from Solanum lycopersicum" . | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 23 . 19 (2022) . |
| APA | Liu, Guangyuan , Meng, Xue , Ren, Yujun , Zhang, Min , Chen, Ziqing , Zhang, Zhaoqi et al. Genes, Structural, and Biochemical Characterization of Four Chlorophyllases from Solanum lycopersicum . | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES , 2022 , 23 (19) . |
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A link between the scent and color of Narcissus tazetta flowers can be anticipated due to their biochemical origin, as well as their similar biological role. Despite the obvious aesthetic and ecological significance of these colorful and fragrant components of the flowers and the molecular profiles of their pigments, fragrant formation has addressed in some cases. However, the regulatory mechanism of the correlation of fragrant components and color patterns is less clear. We simultaneously used one way to address how floral color and fragrant formation in different tissues are generated during the development of an individual plant by transcriptome-based weighted gene co-expression network analysis (WGCNA). A spatiotemporal pattern variation of flavonols/carotenoids/chlorophyll pigmentation and benzenoid/phenylpropanoid/ monoterpene fragrant components between the tepal and corona in the flower tissues of Narcissus tazetta, was exhibited. Several candidate transcription factors: MYB12, MYB1, AP2-ERF, bZIP, NAC, MYB, C2C2, C2H2 and GRAS are shown to be associated with metabolite flux, the phenylpropanoid pathway to the production of flavonols/anthocyanin, as well as related to one branch of the phenylpropanoid pathway to the benzenoid/phenylpropanoid component in the tepal and the metabolite flux between the monoterpene and carotenoids biosynthesis pathway in coronas. It indicates that potential competition exists between floral pigment and floral fragrance during Narcissus tazetta individual plant development and evolutionary development.
Keyword ：
floral fragrance floral fragrance floral pigment floral pigment metabolite flux metabolite flux Narcissus tazetta Narcissus tazetta transcription factors transcription factors transcriptome-based WGCNA analysis transcriptome-based WGCNA analysis
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| GB/T 7714 | Yang, Jingwen , Ren, Yujun , Zhang, Deyu et al. Transcriptome-Based WGCNA Analysis Reveals Regulated Metabolite Fluxes between Floral Color and Scent in Narcissus tazetta Flower [J]. | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES , 2021 , 22 (15) . |
| MLA | Yang, Jingwen et al. "Transcriptome-Based WGCNA Analysis Reveals Regulated Metabolite Fluxes between Floral Color and Scent in Narcissus tazetta Flower" . | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 22 . 15 (2021) . |
| APA | Yang, Jingwen , Ren, Yujun , Zhang, Deyu , Chen, Xuewei , Huang, Jiazhi , Xu, Yun et al. Transcriptome-Based WGCNA Analysis Reveals Regulated Metabolite Fluxes between Floral Color and Scent in Narcissus tazetta Flower . | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES , 2021 , 22 (15) . |
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MicroRNAs negatively regulate gene expression by promoting target mRNA cleavage and/or impairing its translation, thereby playing a crucial role in plant development and environmental stress responses. In Arabidopsis, the MIR840 gene is located within the overlapping 3 ' UTR of the PPR and WHIRLY3 (WHY3) genes, both being predicted targets of miR840* and miR840, the short maturation products of MIR840. Gain- and loss-of-function of MIR840 in Arabidopsis resulted in opposite senescence phenotypes. The highest expression levels of the MIR840 precursor transcript pre-miR840 were observed at senescence initiation, and pre-miR840 expression is significantly correlated with a reduction in PPR, but not WHY3, transcript levels. Although a reduction of transcript level of PPR, but not WHY3 transcript levels were not significantly affected by MIR840 overexpression, its protein levels were strongly reduced. Mutating the cleavage sites or replacing the target sequences abolishes the miR840*/miR840-mediated degradation of PPR transcripts and accumulation of WHY3 protein. In support for this, concurrent knockdown of both PPR and WHY3 in wild-type plants resulted in a senescence phenotype resembling that of the MIR840-overexpressing plant. This indicates that both PRR and WHY3 are targets in the MIR840-mediated senescence pathway. Moreover, single knockout mutants of PPR and WHY3 show a convergent upregulated subset of senescence-associated genes, which are also found among those induced by MIR840 overexpression. Our data provide evidence for a regulatory role of MIR840 in plant senescence.
Keyword ：
Arabidopsis Arabidopsis genetics genetics microRNA microRNA post-transcriptional control post-transcriptional control senescence senescence
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| GB/T 7714 | Ren, Yujun , Li, Mengsi , Wang, Wanzhen et al. MicroRNA840 (MIR840) accelerates leaf senescence by targeting the overlapping 3′UTRs of PPR and WHIRLY3 in Arabidopsis thaliana [J]. | PLANT JOURNAL , 2021 , 109 (1) : 126-143 . |
| MLA | Ren, Yujun et al. "MicroRNA840 (MIR840) accelerates leaf senescence by targeting the overlapping 3′UTRs of PPR and WHIRLY3 in Arabidopsis thaliana" . | PLANT JOURNAL 109 . 1 (2021) : 126-143 . |
| APA | Ren, Yujun , Li, Mengsi , Wang, Wanzhen , Lan, Wei , Schenke, Dirk , Cai, Daguang et al. MicroRNA840 (MIR840) accelerates leaf senescence by targeting the overlapping 3′UTRs of PPR and WHIRLY3 in Arabidopsis thaliana . | PLANT JOURNAL , 2021 , 109 (1) , 126-143 . |
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