Query：
学者姓名：庄宇慧
Refining：
Year
Type
Indexed by
Source
Complex
Co-Author
Language
Clean All
Abstract ：
The TEOSINTE-BRANCHED1/CYCLOIDEA/PROLIFERATING-CELL-FACTOR (TCP) gene family, a plant-specific transcription factor family, plays pivotal roles in various processes such as plant growth and development regulation, hormone crosstalk, and stress responses. However, a comprehensive genome-wide identification and characterization of the TCP gene family in peanut has yet to be fully elucidated. In this study, we conducted a genome-wide search and identified 51 TCP genes (designated as AhTCPs) in peanut, unevenly distributed across 17 chromosomes. These AhTCPs were phylogenetically classified into three subclasses: PCF, CIN, and CYC/TB1. Gene structure analysis of the AhTCPs revealed that most AhTCPs within the same subclade exhibited conserved motifs and domains, as well as similar gene structures. Cis-acting element analysis demonstrated that the AhTCP genes harbored numerous cis-acting elements associated with stress response, plant growth and development, plant hormone response, and light response. Intraspecific collinearity analysis unveiled significant collinear relationships among 32 pairs of these genes. Further collinear evolutionary analysis found that peanuts share 30 pairs, 24 pairs, 33 pairs, and 100 pairs of homologous genes with A. duranensis, A. ipaensis, Arabidopsis thaliana, and Glycine max, respectively. Moreover, we conducted a thorough analysis of the transcriptome expression profiles in peanuts across various tissues, under different hormone treatment conditions, in response to low- and high-calcium treatments, and under low-temperature and drought stress scenarios. The qRT-PCR results were in accordance with the transcriptome expression data. Collectively, these studies have established a solid theoretical foundation for further exploring the biological functions of the TCP gene family in peanuts, providing valuable insights into the regulatory mechanisms of plant growth, development, and stress responses.
Keyword ：
bioinformatics bioinformatics expression pattern analysis expression pattern analysis genome-wide identification genome-wide identification peanut peanut TCP gene TCP gene
Cite：
Copy from the list or Export to your reference management。
| GB/T 7714 | Zhu, Yanting , Niu, Sijie , Lin, Jingyi et al. Genome-Wide Identification and Expression Analysis of TCP Transcription Factors Responding to Multiple Stresses in Arachis hypogaea L. [J]. | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES , 2025 , 26 (3) . |
| MLA | Zhu, Yanting et al. "Genome-Wide Identification and Expression Analysis of TCP Transcription Factors Responding to Multiple Stresses in Arachis hypogaea L." . | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 26 . 3 (2025) . |
| APA | Zhu, Yanting , Niu, Sijie , Lin, Jingyi , Yang, Hua , Zhou, Xun , Wang, Siwei et al. Genome-Wide Identification and Expression Analysis of TCP Transcription Factors Responding to Multiple Stresses in Arachis hypogaea L. . | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES , 2025 , 26 (3) . |
| Export to | NoteExpress RIS BibTex |
Version ：
Abstract ：
Passion fruit (Passiflora edulis), mainly distributed in tropical and subtropical regions, is popular for its unique flavor and health benefits. The actin cytoskeleton plays a crucial role in plant growth and development, and villin is a key regulator of actin dynamics. However, the mechanism underlying the actin filament regulation of reproductive development in passion fruit remains poorly understood. Here, we characterized a villin isovariant in passion fruit, Passiflora edulis VLN4 (PeVLN4), highly and preferentially expressed in pollen. Subcellular localization analysis showed that PeVLN4 decorated distinct filamentous structures in pollen tubes. We next introduced PeVLN4 into Arabidopsis villin mutants to explore its functions on the growing pollen tubes. PeVLN4 rescued defects in the elongation of villin mutant pollen tubes. Pollen tubes expressing PeVLN4 were revealed to be less sensitive to latrunculin B, and PeVLN4 partially rescued defects in the actin filament organization of villin mutant pollen tubes. Additionally, biochemical assays revealed that PeVLN4 bundles actin filaments in vitro. Thus, PeVLN4 is an important regulator of F-actin stability and is required for normal pollen tube growth in passion fruit. This study provides a new insight into the function of the actin regulator villin involved in the reproduction development of passion fruit.
Keyword ：
actin filament actin filament Passiflora edulis Passiflora edulis reproduction development reproduction development VLN gene VLN gene
Cite：
Copy from the list or Export to your reference management。
| GB/T 7714 | Yang, Hanbing , Wei, Xiuqing , Wang, Lifeng et al. Functional Characterization of PeVLN4 Involved in Regulating Pollen Tube Growth from Passion Fruit [J]. | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES , 2025 , 26 (5) . |
| MLA | Yang, Hanbing et al. "Functional Characterization of PeVLN4 Involved in Regulating Pollen Tube Growth from Passion Fruit" . | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 26 . 5 (2025) . |
| APA | Yang, Hanbing , Wei, Xiuqing , Wang, Lifeng , Zheng, Ping , Li, Junzhang , Zou, Yutong et al. Functional Characterization of PeVLN4 Involved in Regulating Pollen Tube Growth from Passion Fruit . | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES , 2025 , 26 (5) . |
| Export to | NoteExpress RIS BibTex |
Version ：
Abstract ：
本发明属于植物基因工程技术领域，具体公开了一种花生糖转运蛋白基因AhSUT4及其在改善花生的味道和质量中的应用。所述基因AhSUT4的核苷酸序列如SEQ ID No.1所示，其编码蛋白的氨基酸序列如SEQ ID No.2所示。通过Gateway系统构建CaMV 35S启动子驱动的过量表达载体经农杆菌介导转化野生型拟南芥哥伦比亚零，通过对转基因植株进行分子检测和可溶性糖含量鉴定，其在拟南芥中超量表达可显著提高转基因拟南芥可溶性糖含量，表明AhSUT4可能参与了植物甜卸载和糖积累反应。本发明为利用基因工程手段培育高甜花生等新品种提供了重要的基因资源，具有重要的应用前景。
Cite：
Copy from the list or Export to your reference management。
| GB/T 7714 | 蔡铁城 , 潘益惊 , 庄伟建 et al. 一种花生糖转运蛋白基因AhSUT4及其应用 : CN202510550148.0[P]. | 2025-04-29 . |
| MLA | 蔡铁城 et al. "一种花生糖转运蛋白基因AhSUT4及其应用" : CN202510550148.0. | 2025-04-29 . |
| APA | 蔡铁城 , 潘益惊 , 庄伟建 , 张冲 , 熊发前 , 陈浪 et al. 一种花生糖转运蛋白基因AhSUT4及其应用 : CN202510550148.0. | 2025-04-29 . |
| Export to | NoteExpress RIS BibTex |
Version ：
Abstract ：
本发明提供一种花生抗青枯病NBS‑LRR编码基因AhRRS6及其应用，所述基因AhRRS6的核苷酸序列如SEQ ID NO.1（来自抗病品种YY92的基因组）和SEQ ID NO.2（来自感病品种XHXL的基因组）所示。所述基因AhRRS6（SEQ ID NO.1）异源超表达烟草显著提高烟草对青枯病的抗性。通过构建AhRRS6的超表达载体转基因本氏烟草，在青枯菌侵染下，来自抗病品种的AhRRS6y与感病品种的AhRRS6x基因及非转基因植株相比，抗病品种转基因植株表现明显抗病性，而感病品种的等位基因和非转基因对照表现感病。此发明为植物抗青枯病的基因工程育种提供了基因资源和理论基础。
Cite：
Copy from the list or Export to your reference management。
| GB/T 7714 | 庄伟建 , 付辉文 , 庄宇慧 et al. 一种花生抗青枯病NBS-LRR编码基因AhRRS6及其应用 : CN202510305464.1[P]. | 2025-03-14 . |
| MLA | 庄伟建 et al. "一种花生抗青枯病NBS-LRR编码基因AhRRS6及其应用" : CN202510305464.1. | 2025-03-14 . |
| APA | 庄伟建 , 付辉文 , 庄宇慧 , 张冲 , 陈华 , 蔡铁城 et al. 一种花生抗青枯病NBS-LRR编码基因AhRRS6及其应用 : CN202510305464.1. | 2025-03-14 . |
| Export to | NoteExpress RIS BibTex |
Version ：
Abstract ：
Bacterial wilt caused by Ralstonia solanacearum is a devastating disease affecting a great many crops including peanut. The pathogen damages plants via secreting type & SHcy; effector proteins (T3Es) into hosts for pathogenicity. Here, we characterized RipAU was among the most toxic effectors as Delta RipAU completely lost its pathogenicity to peanuts. A serine residue of RipAU is the critical site for cell death. The RipAU targeted a subtilisin-like protease (AhSBT1.7) in peanut and both protein moved into nucleus. Heterotic expression of AhSBT1.7 in transgenic tobacco and Arabidopsis thaliana significantly improved the resistance to R. solanacearum. The enhanced resistance was linked with the upregulating ERF1 defense marker genes and decreasing pectin methylesterase (PME) activity like PME2&4 in cell wall pathways. The RipAU played toxic effect by repressing R-gene, defense hormone signaling, and AhSBTs metabolic pathways but increasing PMEs expressions. Furthermore, we discovered AhSBT1.7 interacted with AhPME4 and was colocalized at nucleus. The AhPME speeded plants susceptibility to pathogen via mediated cell wall degradation, which inhibited by AhSBT1.7 but upregulated by RipAU. Collectively, RipAU impaired AhSBT1.7 defense for pathogenicity by using PME-mediated cell wall degradation. This study reveals the mechanism of RipAU pathogenicity and AhSBT1.7 resistance, highlighting peanut immunity to bacterial wilt for future improvement.
Keyword ：
AhPME4 AhPME4 AhSBT1.7 AhSBT1.7 pathogenicity pathogenicity peanut peanut Ralstonia solanacearum Ralstonia solanacearum RipAU RipAU
Cite：
Copy from the list or Export to your reference management。
| GB/T 7714 | Chen, Kun , Zhuang, Yuhui , Chen, Hua et al. A Ralstonia effector RipAU impairs peanut AhSBT1.7 immunity for pathogenicity via AhPME-mediated cell wall degradation [J]. | PLANT JOURNAL , 2025 , 121 (2) . |
| MLA | Chen, Kun et al. "A Ralstonia effector RipAU impairs peanut AhSBT1.7 immunity for pathogenicity via AhPME-mediated cell wall degradation" . | PLANT JOURNAL 121 . 2 (2025) . |
| APA | Chen, Kun , Zhuang, Yuhui , Chen, Hua , Lei, Taijie , Li, Mengke , Wang, Shanshan et al. A Ralstonia effector RipAU impairs peanut AhSBT1.7 immunity for pathogenicity via AhPME-mediated cell wall degradation . | PLANT JOURNAL , 2025 , 121 (2) . |
| Export to | NoteExpress RIS BibTex |
Version ：
Abstract ：
Key messageIntegrating GAB methods with high-throughput phenotyping, genome editing, and speed breeding hold great potential in designing future smart peanut cultivars to meet market and food supply demands.AbstractCultivated peanut (Arachis hypogaea L.), a legume crop greatly valued for its nourishing food, cooking oil, and fodder, is extensively grown worldwide. Despite decades of classical breeding efforts, the actual on-farm yield of peanut remains below its potential productivity due to the complicated interplay of genotype, environment, and management factors, as well as their intricate interactions. Integrating modern genomics tools into crop breeding is necessary to fast-track breeding efficiency and rapid progress. When combined with speed breeding methods, this integration can substantially accelerate the breeding process, leading to faster access of improved varieties to farmers. Availability of high-quality reference genomes for wild diploid progenitors and cultivated peanuts has accelerated the process of gene/quantitative locus discovery, developing markers and genotyping assays as well as a few molecular breeding products with improved resistance and oil quality. The use of new breeding tools, e.g., genomic selection, haplotype-based breeding, speed breeding, high-throughput phenotyping, and genome editing, is probable to boost genetic gains in peanut. Moreover, renewed attention to efficient selection and exploitation of targeted genetic resources is also needed to design high-quality and high-yielding peanut cultivars with main adaptation attributes. In this context, the combination of genomics-assisted breeding (GAB), genome editing, and speed breeding hold great potential in designing future improved peanut cultivars to meet market and food supply demands.
Cite：
Copy from the list or Export to your reference management。
| GB/T 7714 | Raza, Ali , Chen, Hua , Zhang, Chong et al. Designing future peanut: the power of genomics-assisted breeding [J]. | THEORETICAL AND APPLIED GENETICS , 2024 , 137 (3) . |
| MLA | Raza, Ali et al. "Designing future peanut: the power of genomics-assisted breeding" . | THEORETICAL AND APPLIED GENETICS 137 . 3 (2024) . |
| APA | Raza, Ali , Chen, Hua , Zhang, Chong , Zhuang, Yuhui , Sharif, Yasir , Cai, Tiecheng et al. Designing future peanut: the power of genomics-assisted breeding . | THEORETICAL AND APPLIED GENETICS , 2024 , 137 (3) . |
| Export to | NoteExpress RIS BibTex |
Version ：
Abstract ：
Alternative splicing (AS), an important post-transcriptional regulation mechanism in eukaryotes, can significantly increase transcript diversity and contribute to gene expression regulation and many other complicated developmental processes. While plant gene AS events are well described, few studies have investigated the comprehensive regulation machinery of plant AS. Here, we use multi-omics to analyse peanut AS events. Using long-read isoform sequencing, 146 464 full-length non-chimeric transcripts were obtained, resulting in annotation corrections for 1782 genes and the identification of 4653 new loci. Using Iso-Seq RNA sequences, 271 776 unique splice junctions were identified, 82.49% of which were supported by transcriptome data. We characterized 50 977 polyadenylation sites for 23 262 genes, 12 369 of which had alternative polyadenylation sites. AS allows differential regulation of the same gene by miRNAs at the isoform level coupled with polyadenylation. In addition, we identified many long non-coding RNAs and fusion transcripts. There is a suppressed effect of 6mA on AS and gene expression. By analysis of chromatin structures, the genes located in the boundaries of topologically associated domains, proximal chromosomal telomere regions, inter- or intra-chromosomal loops were found to have more unique splice isoforms, higher expression, lower 6mA and more transposable elements (TEs) in their gene bodies than the other genes, indicating that chromatin interaction, 6mA and TEs play important roles in AS and gene expression. These results greatly refine the peanut genome annotation and contribute to the study of gene expression and regulation in peanuts. This work also showed AS is associated with multiple strategies for gene regulation.
Keyword ：
6mA 6mA alternative splicing alternative splicing chromatin structures chromatin structures Iso-Seq Iso-Seq peanut peanut
Cite：
Copy from the list or Export to your reference management。
| GB/T 7714 | Wang, Lihui , Chen, Hua , Zhuang, Yuhui et al. Multiple strategies, including 6mA methylation, affecting plant alternative splicing in allopolyploid peanut [J]. | PLANT BIOTECHNOLOGY JOURNAL , 2024 , 22 (6) : 1681-1702 . |
| MLA | Wang, Lihui et al. "Multiple strategies, including 6mA methylation, affecting plant alternative splicing in allopolyploid peanut" . | PLANT BIOTECHNOLOGY JOURNAL 22 . 6 (2024) : 1681-1702 . |
| APA | Wang, Lihui , Chen, Hua , Zhuang, Yuhui , Chen, Kun , Zhang, Chong , Cai, Tiecheng et al. Multiple strategies, including 6mA methylation, affecting plant alternative splicing in allopolyploid peanut . | PLANT BIOTECHNOLOGY JOURNAL , 2024 , 22 (6) , 1681-1702 . |
| Export to | NoteExpress RIS BibTex |
Version ：
Abstract ：
Being a bona fide actin bundler, Arabidopsis villin5 (VLN5) plays a crucial role in regulating actin stability and organization within pollen tubes. Despite its significance, the precise mechanism through which VLN5 bundles actin filaments has remained elusive. Through meticulous deletion analysis, we have unveiled that the link between gelsolin repeat 6 (G6) and the headpiece domain (VHP), rather than VHP itself, is indispensable for VLN5-mediated actin bundling. Further refinement of this region has pinpointed a critical sequence spanning from Val763 to Ser823, essential for VLN5's actin-bundling activity. Notably, the absence of Val763-Ser823 in VLN5 results in decreased filamentous decoration within pollen tubes and a diminished ability to rescue actin bundling defects in vln2vln5 mutant pollen tubes compared to intact VLN5. Moreover, our findings highlight that the Val763-Ser823 sequence harbors a binding site for F-actin, suggesting that this linker-based F-actin binding site, in conjunction with the F-actin binding site localized in G1-G6, enables a single VLN5 to concurrently bind to two adjacent actin filaments. Therefore, our study unveils a novel mechanism by which VLN5 bundles actin filaments.
Keyword ：
actin bundles actin bundles pollen tube pollen tube villin villin villin headpiece villin headpiece villin linker villin linker
Cite：
Copy from the list or Export to your reference management。
| GB/T 7714 | Zhuang, Yuhui , Wang, Yingjie , Jiao, Cuixia et al. Arabidopsis VILLIN5 bundles actin filaments using a novel mechanism [J]. | PLANT JOURNAL , 2024 , 119 (6) : 2854-2866 . |
| MLA | Zhuang, Yuhui et al. "Arabidopsis VILLIN5 bundles actin filaments using a novel mechanism" . | PLANT JOURNAL 119 . 6 (2024) : 2854-2866 . |
| APA | Zhuang, Yuhui , Wang, Yingjie , Jiao, Cuixia , Shang, Zhonglin , Huang, Shanjin . Arabidopsis VILLIN5 bundles actin filaments using a novel mechanism . | PLANT JOURNAL , 2024 , 119 (6) , 2854-2866 . |
| Export to | NoteExpress RIS BibTex |
Version ：
Abstract ：
Cultivated peanut (Arachis hypogaea L.) is a key oil- and protein-providing legume crop of the world. It is full of nutrients, and its nutrient profile is comparable to that of other nuts. Peanut is a unique plant as it showcases a pegging phenomenon, producing flowers above ground, and after fertilization, the developing peg enters the soil and produces seeds underground. This geocarpic nature of peanut exposes its seeds to soil pathogens. Peanut seeds are protected by an inedible pericarp and testa. The pericarp- and testa-specific promoters can be effectively used to improve the seed defense. We identified a pericarp- and testa-abundant expression gene (AhN8DT-2) from available transcriptome expression data, whose tissue-specific expression was further confirmed by the qRT-PCR. The 1827bp promoter sequence was used to construct the expression vector using the pMDC164 vector for further analysis. Quantitative expression of the GUS gene in transgenic Arabidopsis plants showed its high expression in the pericarp. GUS staining showed a deep blue color in the pericarp and testa. Cryostat sectioning of stained Arabidopsis seeds showed that expression is only limited to seed coat (testa), and staining was not present in cotyledons and embryos. GUS staining was not detected in any other tissues, including seedlings, leaves, stems, and roots, except for some staining in flowers. Under different phytohormones, this promoter did not show an increase in expression level. These results indicated that the AhN8DT-2 promoter drives GUS gene expression in a pericarp- and testa-specific manner. The identified promoter can be utilized to drive disease resistance genes, specifically in the pericarp and testa, enhancing peanut seed defense against soil-borne pathogens. This approach has broader implications for improving the resilience of peanut crops and other legumes, contributing to sustainable agricultural practices and food security.
Keyword ：
abiotic stress abiotic stress aflatoxins aflatoxins constitutive promoter constitutive promoter genome-wide genome-wide tissue-specific promoter tissue-specific promoter
Cite：
Copy from the list or Export to your reference management。
| GB/T 7714 | Sharif, Yasir , Zhuang, Yuhui , Xie, Wenpin et al. Molecular Cloning and Functional Identification of a Pericarp- and Testa-Abundant Gene's (AhN8DT-2) Promoter from Arachis hypogaea [J]. | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES , 2024 , 25 (14) . |
| MLA | Sharif, Yasir et al. "Molecular Cloning and Functional Identification of a Pericarp- and Testa-Abundant Gene's (AhN8DT-2) Promoter from Arachis hypogaea" . | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 25 . 14 (2024) . |
| APA | Sharif, Yasir , Zhuang, Yuhui , Xie, Wenpin , Zhang, Chong , Chen, Kun , Deng, Ye et al. Molecular Cloning and Functional Identification of a Pericarp- and Testa-Abundant Gene's (AhN8DT-2) Promoter from Arachis hypogaea . | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES , 2024 , 25 (14) . |
| Export to | NoteExpress RIS BibTex |
Version ：
Abstract ：
本发明公开了一种花生青枯菌RS‑P.362200中的效应蛋白RipTAL及其编码基因和应用，属于植物病理学与分子生物学研究领域。所述青枯菌效应蛋白RipTAL核苷酸序列如SEQ ID No.1所示，编码的氨基酸序列如SEQ ID No.2所示。利用同源重组法分别在野生型青枯菌RS‑P.362200中敲除RipTAL及其转录激活区AD，构建了青枯菌RipTAL缺失突变体ΔRipTAL及AD缺失突变体ΔRipTAL‑AD，将ΔRipTAL与ΔRipTAL‑AD接种花生表现致病力减弱，表明RipTAL是一个毒力因子，在花生与青枯菌互作中发挥重要作用，且AD区对RipTAL的致病力起着十分关键的作用。通过转基因本氏烟草株系与野生型植株分别接种青枯菌比较分析发现，RipTAL在本氏烟草中超量表达可显著提高烟草对青枯菌的抗性。本发明对于解析RipTAL介导的青枯菌‑花生互作机理及指导抗青枯病花生分子育种具有重要意义。
Cite：
Copy from the list or Export to your reference management。
| GB/T 7714 | 陈华 , 陆文智 , 林婧怡 et al. 一种花生青枯菌效应蛋白RipTAL及其在植物抗青枯病中的应用 : CN202311763214.X[P]. | 2023-12-20 . |
| MLA | 陈华 et al. "一种花生青枯菌效应蛋白RipTAL及其在植物抗青枯病中的应用" : CN202311763214.X. | 2023-12-20 . |
| APA | 陈华 , 陆文智 , 林婧怡 , 杨强 , 朱艳婷 , 杨欢 et al. 一种花生青枯菌效应蛋白RipTAL及其在植物抗青枯病中的应用 : CN202311763214.X. | 2023-12-20 . |
| Export to | NoteExpress RIS BibTex |
Version ：
Export
| Results： |
Selected to |
| Format： |
