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学者姓名：王宇
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Pathogenic filamentous fungi pose a significant threat to global food security and human health. The limitations of available antifungal agents, including resistance and toxicity, highlight the need for developing innovative antifungal strategies. Antifungal proteins (AFPs) are a class of secreted small proteins that exhibit potent antifungal activity against filamentous fungi, yet the underlying mechanism remains partially understood. In this study, we investigate the molecular and cellular effects of two AFPs, PgAFP and AfAFP, on Aspergillus flavus, a representative filamentous fungus. These AFPs affect various fungal phenotypes and exert an intracellular effect by interacting with Ntp1, a fungi exclusive protein modulating diverse fungal traits. We find that Ntp1 amino acids 417-588 are critical for AFP binding and play a role in regulating growth, development, sporulation, sclerotia formation, toxin synthesis, and pathogenicity. Results generated from this study will help to control pathogenic fungi.
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| GB/T 7714 | Wang, Yu , Wang, Sen , Chen, Yuanyuan et al. The role of Npt1 in regulating antifungal protein activity in filamentous fungi [J]. | NATURE COMMUNICATIONS , 2025 , 16 (1) . |
| MLA | Wang, Yu et al. "The role of Npt1 in regulating antifungal protein activity in filamentous fungi" . | NATURE COMMUNICATIONS 16 . 1 (2025) . |
| APA | Wang, Yu , Wang, Sen , Chen, Yuanyuan , Xie, Chunlan , Xu, Haibo , Lin, Yunhua et al. The role of Npt1 in regulating antifungal protein activity in filamentous fungi . | NATURE COMMUNICATIONS , 2025 , 16 (1) . |
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Filamentous fungi pose a significant threat to global food security and human well-being due to their potent pathogenicity and the resulting infectious diseases.Limitations associated with current antifungal agents,such as resistance development and toxicity concerns,necessitate the pursuit of innovative antifungal strategies.Antifungal proteins(AFPs) are a class of secreted small proteins exhibiting potent antifungal activity against filamentous fungi.Despite their discovery several decades ago,the precise mechanisms underlying their antifungal effects remain partially elucidated.In this study,we investigated the molecular and cellular consequences of two AFPs,PgAFP and AfAFP,on Aspergillus flavus(A.flavus),a representative filamentous fungus.We found that these two AFPs not only affect various fungal phenotypes,but also exert a novel intracellular effect by interacting with a newly identified protein,Ntp1,which modulates diverse fungal traits.Furthermore,we identified amino acids417-588 within Ntp1 as cruci...
Keyword ：
AFP-interacting proteins AFP-interacting proteins Antifungal Protein Antifungal Protein Aspergillus flavus Aspergillus flavus Filamentous Fungi Filamentous Fungi Ntp1 Ntp1
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| GB/T 7714 | 王宇 , 王森 , 陈媛媛 et al. Insights from the Microorganism-Specific Protein Ntp1 in Regulating Antifungal Protein Activity in Filamentous Fungi [C] //中国菌物学会2024年学术年会 . 2024 . |
| MLA | 王宇 et al. "Insights from the Microorganism-Specific Protein Ntp1 in Regulating Antifungal Protein Activity in Filamentous Fungi" 中国菌物学会2024年学术年会 . (2024) . |
| APA | 王宇 , 王森 , 陈媛媛 , 谢春兰 , 林云华 , 林染荀 et al. Insights from the Microorganism-Specific Protein Ntp1 in Regulating Antifungal Protein Activity in Filamentous Fungi 中国菌物学会2024年学术年会 . (2024) . |
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Lysophospholipids (lysoPLs) are crucial metabolites involved in various physiological and pathological cellular processes. Understanding their binding interactions, particularly with human serum albumin (HSA), is essential due to their role in regulating lysoPLs-induced cytotoxicity. However, the precise mechanism of lysoPLs binding to HSA remains elusive. In this study, we employed fluorescence quenching and optical interferometry assays to demonstrate direct binding between with LPC, both in the absence and the presence of the endogenous fatty acid myristate (14:0). The crystal structure of binary HSA:LPC revealed that six LPC molecules are bound to HSA at the primary fatty acid binding sites. Interestingly, the ternary HSA:Myr:LPC structure demonstrated the continued binding of three LPC molecules to HSA at binding sites 1, 3, and 5 in the presence of myristate. These findings support HSA's role as a carrier protein for lysoPLs in blood plasma and provide valuable insights into the structural basis of their binding mechanisms.
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| GB/T 7714 | Wang, Yu , Luo, Zhipu , Morelli, Xavier et al. Crystal structures of human serum albumin in complex with lysophosphatidylcholine [J]. | BIOPHYSICAL JOURNAL , 2023 , 122 (21) : 4135-4143 . |
| MLA | Wang, Yu et al. "Crystal structures of human serum albumin in complex with lysophosphatidylcholine" . | BIOPHYSICAL JOURNAL 122 . 21 (2023) : 4135-4143 . |
| APA | Wang, Yu , Luo, Zhipu , Morelli, Xavier , Xu, Peng , Jiang, Longguang , Shi, Xiaoli et al. Crystal structures of human serum albumin in complex with lysophosphatidylcholine . | BIOPHYSICAL JOURNAL , 2023 , 122 (21) , 4135-4143 . |
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Soil salinity is a growing concern for global crop production and the sustainable development of humanity. Therefore, it is crucial to comprehend salt tolerance mechanisms and identify salt-tolerance genes to enhance crop tolerance to salt stress. Suaeda glauca, a halophyte species well adapted to the seawater environment, possesses a unique ability to absorb and retain high salt concentrations within its cells, particularly in its leaves, suggesting the presence of a distinct mechanism for salt tolerance. In this study, we performed de novo sequencing of the S. glauca genome. The genome has a size of 1.02 Gb (consisting of two sets of haplotypes) and contains 54 761 annotated genes, including alleles and repeats. Comparative genomic analysis revealed a strong synteny between the genomes of S. glauca and Beta vulgaris. Of the S. glauca genome, 70.56% comprises repeat sequences, with retroelements being the most abundant. Leveraging the allele-aware assembly of the S. glauca genome, we investigated genome-wide allele-specific expression in the analyzed samples. The results indicated that the diversity in promoter sequences might contribute to consistent allele-specific expression. Moreover, a systematic analysis of the ABCE gene families shed light on the formation of S. glauca's flower morphology, suggesting that dysfunction of A-class genes is responsible for the absence of petals in S. glauca. Gene family expansion analysis demonstrated significant enrichment of Gene Ontology (GO) terms associated with DNA repair, chromosome stability, DNA demethylation, cation binding, and red/far-red light signaling pathways in the co-expanded gene families of S. glauca and S. aralocaspica, in comparison with glycophytic species within the chenopodium family. Time-course transcriptome analysis under salt treatments revealed detailed responses of S. glauca to salt tolerance, and the enrichment of the transition-upregulated genes in the leaves associated with DNA repair and chromosome stability, lipid biosynthetic process, and isoprenoid metabolic process. Additionally, genome-wide analysis of transcription factors indicated a significant expansion of FAR' gene family. However, further investigation is needed to determine the exact role of the FAR' gene family in salt tolerance in S. glauca.
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| GB/T 7714 | Cheng, Yan , Sun, Jin , Jiang, Mengwei et al. Chromosome-scale genome sequence of Suaeda glauca sheds light on salt stress tolerance in halophytes [J]. | HORTICULTURE RESEARCH , 2023 , 10 (9) . |
| MLA | Cheng, Yan et al. "Chromosome-scale genome sequence of Suaeda glauca sheds light on salt stress tolerance in halophytes" . | HORTICULTURE RESEARCH 10 . 9 (2023) . |
| APA | Cheng, Yan , Sun, Jin , Jiang, Mengwei , Luo, Zigiang , Wang, Yu , Liu, Yanhui et al. Chromosome-scale genome sequence of Suaeda glauca sheds light on salt stress tolerance in halophytes . | HORTICULTURE RESEARCH , 2023 , 10 (9) . |
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介绍了在生物体内特别是在病原真菌中，参与介导N-肉豆蔻酰化进程中的关键蛋白，即N-肉豆蔻酰基转移酶的作用。同时阐述了N-肉豆蔻酰基转移酶可作为具有广泛应用前景的抗真菌新型靶标的原因。此外，还总结了N-肉豆蔻酰基转移酶的催化作用机理，以及抑制该酶活性的小分子化合物的结构基础，并展望了基于该酶结构开发抗真菌药物的研究前景。
Keyword ：
N-肉豆蔻酰化 N-肉豆蔻酰化 N-肉豆蔻酰基转移酶 N-肉豆蔻酰基转移酶 作用机制 作用机制 抑制剂 抑制剂 抗真菌靶标 抗真菌靶标
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| GB/T 7714 | 林染荀 , 曾菀林 , 刘梦昕 et al. 真菌靶标N-肉豆蔻酰基转移酶的研究进展 [J]. | 福建农林大学学报(自然科学版) , 2023 , 52 (05) : 573-581 . |
| MLA | 林染荀 et al. "真菌靶标N-肉豆蔻酰基转移酶的研究进展" . | 福建农林大学学报(自然科学版) 52 . 05 (2023) : 573-581 . |
| APA | 林染荀 , 曾菀林 , 刘梦昕 , 王森 , 史嘉鹏 , 许海波 et al. 真菌靶标N-肉豆蔻酰基转移酶的研究进展 . | 福建农林大学学报(自然科学版) , 2023 , 52 (05) , 573-581 . |
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SUMO adducts occur in Aspergillus flavus, and are implicated in fungal biology, while the underlying mechanism and the SUMOylation apparatus components in this saprophytic food spoilage mould, remain undefined. Herein, genes encoding SUMOylation cascade enzymes in A. flavus, including two heterodimeric SUMO E1 activating enzymes, a unique SUMO E2 conjugating enzyme, and one of SUMO E3 ligases, were identified and functionally analyzed. Global SUMO adducts immunoassay, multiple morphological comparison, aflatoxin attributes test, fungal infection and transcriptomic analyses collectively revealed that: E1 and E2 were essential for intracellular SUMOylation, and contributed to both stress response and fungal virulence-related events, including sporulation, colonization, aflatoxins biosynthesis; the primary E3 in this fungus, AfSizA, might serve as the molecular linkage of SUMOylation pathway to fungal virulence rather than SUMOylation-mediated stress adaptation. These findings demonstrated that SUMOylation machinery in A. flavus was functionally intact and contributed to multiple pathobiological processes, hence offering ideas and targets to control food contamination by this mycotoxigenic fungus.
Keyword ：
Aspergillus flavus Aspergillus flavus E1 activating enzyme E1 activating enzyme E2 conjugating enzyme E2 conjugating enzyme E3 ligase E3 ligase Food contamination Food contamination SUMOylation SUMOylation
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| GB/T 7714 | Nie, Xin-Yi , Xue, Yang , Li, Ling et al. A functional intact SUMOylation machinery in Aspergillus flavus contributes to fungal and aflatoxin contamination of food [J]. | INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY , 2023 , 398 . |
| MLA | Nie, Xin-Yi et al. "A functional intact SUMOylation machinery in Aspergillus flavus contributes to fungal and aflatoxin contamination of food" . | INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY 398 (2023) . |
| APA | Nie, Xin-Yi , Xue, Yang , Li, Ling , Jiang, Zhixin , Qin, Bei , Wang, Yu et al. A functional intact SUMOylation machinery in Aspergillus flavus contributes to fungal and aflatoxin contamination of food . | INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY , 2023 , 398 . |
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Soil acidification is common in some Chinese tea plantations, which seriously affected growth of tea trees. Hence, it is essential to explore soil remediation in acidified tea plantations for sustainable development of the tea industry. We sought to determine how different fertilizers affect acidified soil and their N transformation in tea plantations.Different fertilizers were used on acidified tea plantation soils for 4 years (2017-2021), and changes in soil pH, indices related to soil N transformation and tea yield were analysed to construct interaction networks of these indices and find which had the largest influence on fertilization.Long-term use of sheep manure reduced soil acidification, increased soil pH, enhanced the number and intensity of N-fixing and ammonifying bacteria, urease, protease, asparaginase and N-acetamide glucose ribosidase activity and nifH gene expression. This treatment reduced the number and intensity of soil nitrifying and denitrifying bacteria, nitrate reductase and nitrite reductase activity, while the expression of amoA-AOA, nirK, nirS, narG and nosZ in turn increased ammonium N content of the soil, reduced nitrate N content, and enhanced tea yield. Topsis index weight analysis showed that ammonium N content in the soil had the largest impact among fertilization effects.Long-term use of sheep manure was beneficial in restoring the balance of the micro-ecosystem in acidified soil. This study provides an important practical basis for soil remediation and fertilizer management in acidified tea plantation soils.
Keyword ：
gene expression gene expression microorganisms microorganisms nitrogen transformation nitrogen transformation sheep manure fertilizer sheep manure fertilizer soil acidification soil acidification soil enzymes soil enzymes tea plantation tea plantation
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| GB/T 7714 | Ye, J. , Wang, Y. , Hong, L. et al. Improvement of soil acidification and ammonium nitrogen content in tea plantations by long-term use of organic fertilizer [J]. | PLANT BIOLOGY , 2023 , 25 (6) : 994-1008 . |
| MLA | Ye, J. et al. "Improvement of soil acidification and ammonium nitrogen content in tea plantations by long-term use of organic fertilizer" . | PLANT BIOLOGY 25 . 6 (2023) : 994-1008 . |
| APA | Ye, J. , Wang, Y. , Hong, L. , Kang, J. , Jia, Y. , Li, M. et al. Improvement of soil acidification and ammonium nitrogen content in tea plantations by long-term use of organic fertilizer . | PLANT BIOLOGY , 2023 , 25 (6) , 994-1008 . |
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In the Convolvulaceae family, around 1650 species belonging to 60 genera are widely distributed globally, mainly in the tropical and subtropical regions of America and Asia. Although a series of chloroplast genomes in Convolvulaceae were reported and investigated, the evolutionary and genetic relationships among the chloroplast genomes of the Convolvulaceae family have not been extensively elucidated till now. In this study, we first reported the complete chloroplast genome sequence of Ipomoea pes-caprae, a widely distributed coastal plant with medical values. The chloroplast genome of I. pes-caprae is 161667 bp in length, and the GC content is 37.56%. The chloroplastic DNA molecule of I. pes-caprae is a circular structure composed of LSC (large-single-copy), SSC (small-single-copy), and IR (inverted repeat) regions, with the size of the three regions being 88210 bp, 12117 bp, and 30670 bp, respectively. The chloroplast genome of I. pes-caprae contains 141 genes, and 35 SSRs are identified in the chloroplast genome. Our research results provide important genomic information for the molecular phylogeny of I. pes-caprae. The Phylogenetic analysis of 28 Convolvulaceae chloroplast genomes showed that the relationship of I. pes-caprae with I. involucrata or I. obscura was much closer than that with other Convolvulaccae species. Further comparative analyses between the Ipomoea species and Cuscuta species revealed the mechanism underlying the formation of parasitic characteristics of Cuscuta species from the perspective of the chloroplast genome.
Keyword ：
chloroplast genome chloroplast genome Convolvulaceae Convolvulaceae gene loss gene loss parasitic plants parasitic plants photosynthesis photosynthesis
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| GB/T 7714 | Wang, Yu , Xu, Jing , Hu, Bin et al. Assembly, annotation, and comparative analysis of Ipomoea chloroplast genomes provide insights into the parasitic characteristics of Cuscuta species [J]. | FRONTIERS IN PLANT SCIENCE , 2023 , 13 . |
| MLA | Wang, Yu et al. "Assembly, annotation, and comparative analysis of Ipomoea chloroplast genomes provide insights into the parasitic characteristics of Cuscuta species" . | FRONTIERS IN PLANT SCIENCE 13 (2023) . |
| APA | Wang, Yu , Xu, Jing , Hu, Bin , Dong, Chunxing , Sun, Jin , Li, Zixian et al. Assembly, annotation, and comparative analysis of Ipomoea chloroplast genomes provide insights into the parasitic characteristics of Cuscuta species . | FRONTIERS IN PLANT SCIENCE , 2023 , 13 . |
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As the main producer of aflatoxins, Aspergillus flavus is also one of the most important causes of invasive and non-invasive aspergillosis. Therefore, it is crucial to unravel the regulatory mechanisms of growth, metabolism, and pathogenicity of A. flavus. SWD1 is highly conserved across species for maintaining COMPASS methyltransferase activity, but the bio-function of SWD1 in A. flavus has not been explored. Through genetic analysis, this study revealed that SWD1 is involved in fungal morphogenesis and AFB1 biosynthesis by regulating the orthodox pathways through H3K4me1-3. Stresses sensitivity and crop models analysis revealed that SWD1 is a key regulator for the resistance of A. flavus to adapt to extreme adverse environments and to colonize crop kernels. It also revealed that the WD40 domain and 25 aa highly conserved sequence are indispensable for SWD1 in the regulation of mycotoxin bio-synthesis and fungal virulence. Metabolomic analysis inferred that SWD1 is crucial for the biosynthesis of numerous primary and secondary metabolites, regulates biological functions by reshaping the whole metabolic process, and may inhibit fungal virulence by inducing the apoptosis of mycelia through the inducer sphingosine. This study elucidates the epigenetic mechanism of SWD1 in regulating fungal pathogenicity and mycotoxin biosynthesis, and provides a potential novel target for controlling the virulence of A. flavus.
Keyword ：
Aflatoxins Aflatoxins Aspergillus flavus Aspergillus flavus Histone methylation Histone methylation Metabolomics Metabolomics SWD1 SWD1
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| GB/T 7714 | Pan, Xiaohua , Hao, Ling , Yang, Chi et al. SWD1 epigenetically chords fungal morphogenesis, aflatoxin biosynthesis, metabolism, and virulence of Aspergillus flavus [J]. | JOURNAL OF HAZARDOUS MATERIALS , 2023 , 455 . |
| MLA | Pan, Xiaohua et al. "SWD1 epigenetically chords fungal morphogenesis, aflatoxin biosynthesis, metabolism, and virulence of Aspergillus flavus" . | JOURNAL OF HAZARDOUS MATERIALS 455 (2023) . |
| APA | Pan, Xiaohua , Hao, Ling , Yang, Chi , Lin, Hong , Wu, Dandan , Chen, Xuan et al. SWD1 epigenetically chords fungal morphogenesis, aflatoxin biosynthesis, metabolism, and virulence of Aspergillus flavus . | JOURNAL OF HAZARDOUS MATERIALS , 2023 , 455 . |
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Adverse environmental conditions often present challenges for organisms; however, they can also serve as selective pressures that propel adaptive evolution. In this study, we present the first chromosome-scale genome of Ipomoea pes-caprae L. (IPC), an exceptionally salt-tolerant plant species of considerable significance due to its medicinal, ecological, and biological attributes. The haplotype IPC genome comprises 15 chromosomes spanning 1.05 GB and includes 34,077 protein-coding genes, exhibiting an impressive completeness of 97.4%. Comparative genomic analysis with non-salt-tolerant Ipomoea species has highlighted the prevalence of highly duplicated sequences and genes within the IPC genome. Analysis of gene ortholog expansion, when compared in those Ipomoea species, reveals that expanded TRD (transposed duplication) and DSD (dispersed duplication) genes are predominantly associated with functions related to salt tolerance. Furthermore, our findings suggest strong correlations between DSD and TRD geness and transposable element (TE) events, implying that TE-induced expansion of repeat genes is a driving force behind gene diversification. Moreover, a time-course RNA-seq analysis unveils the salt response of IPC roots and leaves, showing the involvement of several key salt-tolerance genes exhibiting copy number expansion. These include genes responsible for ion uptake, transportation, and sequestration into vacuoles, as well as genes responsible for the maintenance of DNA and chromosome stability. Given the significant induction of TE events by salt stress in plant genomes, we propose a putative mechanism for the rapid evolution of salt tolerance in IPC. Additionally, this study delves into the metabolic pathway and regulatory mechanisms of Caffeoylquinic acids (CQAs), a medicinal component found in IPC.
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| GB/T 7714 | Cheng, Yan , Wang, Yu , Sun, Jin et al. Unveiling the genomic blueprint of salt stress: insights from Ipomoea pes-caprae L. [J]. | SEED BIOLOGY , 2023 , 2 . |
| MLA | Cheng, Yan et al. "Unveiling the genomic blueprint of salt stress: insights from Ipomoea pes-caprae L." . | SEED BIOLOGY 2 (2023) . |
| APA | Cheng, Yan , Wang, Yu , Sun, Jin , Liao, Zhenyang , Ye, Kangzhuo , Hu, Bin et al. Unveiling the genomic blueprint of salt stress: insights from Ipomoea pes-caprae L. . | SEED BIOLOGY , 2023 , 2 . |
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