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学者姓名：秦源
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Abstract ：
本发明涉及一种调控水稻大孢子发生的OsEPF1基因，具体涉及一个植物富含半胱氨酸的分泌肽OsEPF1参与调控水稻大孢子发生过程。本发明调控水稻大孢子发生的OsEPF1基因功能缺失突变体与野生型ZH11相比，雌性生殖细胞发育存在明显缺陷，特别是在胚珠发育早期，AC和MMC时期，突变体会产生多个AC/MMC‑like的细胞，进而影响育性，导致水稻单株结实率下降；将pEPF1::EPF1回补到突变体后胚珠中大孢子发生过程发育正常，且结实率能够恢复正常。本发明证明OsEPF1通过调控水稻大孢子发生过程中体细胞向生殖细胞命运的转变，从而影响雌配子体发育，最终影响水稻的结实率，这一发现对水稻雌性生殖细胞命运决定的研究具有重要意义，这对水稻育种工作具有一定的应用价值。
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| GB/T 7714 | 秦源 , 郭明亮 , 江淑宇 et al. 一种调控水稻大孢子发生的OsEPF1基因 : CN202411534356.3[P]. | 2024-10-31 . |
| MLA | 秦源 et al. "一种调控水稻大孢子发生的OsEPF1基因" : CN202411534356.3. | 2024-10-31 . |
| APA | 秦源 , 郭明亮 , 江淑宇 , 郑椿 , 田大刚 . 一种调控水稻大孢子发生的OsEPF1基因 : CN202411534356.3. | 2024-10-31 . |
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本发明涉及一种调控水稻雌配子体发育的类受体激酶OsERL基因，以及其参与调控水稻雌配子体发育。所述调控水稻雌配子体发育的OsERL基因功能缺失突变体与野生型ZH11相比，雌性生殖细胞发育存在明显缺陷，进而导致水稻单株结实率下降；将pERL::ERL回补到突变体后胚珠中雌配子体发育正常，且结实率能够恢复正常。本研究证明OsERL通过调控水稻雌配子体发育，从而影响水稻的结实率，这一发现对水稻雌性生殖细胞命运决定的研究具有重要意义，这对水稻育种工作具有一定的应用价值。
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| GB/T 7714 | 秦源 , 郭明亮 , 石超 et al. 一种调控水稻雌配子体发育的类受体激酶OsERL基因 : CN202411534514.5[P]. | 2024-10-31 . |
| MLA | 秦源 et al. "一种调控水稻雌配子体发育的类受体激酶OsERL基因" : CN202411534514.5. | 2024-10-31 . |
| APA | 秦源 , 郭明亮 , 石超 , 严茂凯 , 田大刚 . 一种调控水稻雌配子体发育的类受体激酶OsERL基因 : CN202411534514.5. | 2024-10-31 . |
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本发明涉及一个植物类受体激酶基因OsER1参与调控水稻颖花发育，调控水稻颖花发育及种子形态的OsER1基因功能缺失突变体与野生型相比，oser1‑T的颖片、内外稃、雄蕊、雌蕊、柱头和浆片的数目发生了部分偏离。由于一个小穗中雌蕊数目增多使得种子数目增多，以及种子形态异常的表型。本研究证明OsER1通过调控水稻颖花发育，从而影响水稻的雌蕊数目及种子形态。这一发现将对水稻遗传材料的创制及作物遗传改良工作具有重要意义，这对水稻育种工作具有一定的应用价值。
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| GB/T 7714 | 秦源 , 郭明亮 , 郑椿 et al. 一种调控水稻雌蕊数目及种子形态的类受体激酶OsER1基因 : CN202411534760.0[P]. | 2024-10-31 . |
| MLA | 秦源 et al. "一种调控水稻雌蕊数目及种子形态的类受体激酶OsER1基因" : CN202411534760.0. | 2024-10-31 . |
| APA | 秦源 , 郭明亮 , 郑椿 , 石超 , 田大刚 . 一种调控水稻雌蕊数目及种子形态的类受体激酶OsER1基因 : CN202411534760.0. | 2024-10-31 . |
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菠萝（Ananas comosus）是一种营养丰富、风味独特的水果，为全球贸易量第二大的热带水果，在我国热带农业经济中占据重要地位，广西是我国菠萝的主产区之一。本研究旨在系统评估广西菠萝产业发展现状，识别制约产业升级的关键瓶颈，并提出科学对策，为区域农业供给侧结构性改革与乡村振兴战略提供重要依据。基于2019―2023年广西农业农村厅统计数据，结合菠萝主产区实地调研与CNKI数据库文献分析，采用描述性统计、产业竞争力分析及典型案例研究法，从产业地位和历史沿革、产业布局及品种结构、生产效率与经济效益、生产技术与管理水平、市场销售与品牌建设等维度解析菠萝产业动态。结果表明，2019―2023年，广西菠萝种植面积年均减少了8.9%，但单产提高了79.49%，每公顷产值增长了55.24%，呈现单产水平、单位面积附加值大幅度提高，产业效益显著提升的特征。品种更新比例达40%，台农系列及金菠萝等新品种推广成效显著，标准化生产技术推动每公顷产量突破42 000 kg。然而，品种单一化（巴厘品种占比60%）、寒害与凋萎病频发（年均受灾面积占比31.7%）、加工率低（11.5%）等问题仍制约产业可持续发展。建议通过加强种质创新与优化产业布局，研发新品种配套栽培技术体系并进行示范推广，构建智能化绿色病虫害防控体系，完善采后处理体系、延伸加工产业链、提升产业组织化与市场推广效能等措施，实现广西菠萝产业从“面减质升”向“质效双增”的跨越式发展。本研究的成果可为热带水果产业高质量发展提供参考。
Keyword ：
产业链协同 产业链协同 产期调控 产期调控 广西 广西 菠萝 菠萝 避寒栽培 避寒栽培
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| GB/T 7714 | 邓彪 , 刘开创 , 柴改凤 et al. 广西菠萝产业现状分析及发展对策 [J]. | 农业研究与应用 , 2025 , 38 (03) : 226-234 . |
| MLA | 邓彪 et al. "广西菠萝产业现状分析及发展对策" . | 农业研究与应用 38 . 03 (2025) : 226-234 . |
| APA | 邓彪 , 刘开创 , 柴改凤 , 郑平 , 王路路 , 陶慧 et al. 广西菠萝产业现状分析及发展对策 . | 农业研究与应用 , 2025 , 38 (03) , 226-234 . |
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Passion fruit (Passiflora edulis), mainly distributed in tropical and subtropical regions, is popular for its unique flavor and health benefits. The actin cytoskeleton plays a crucial role in plant growth and development, and villin is a key regulator of actin dynamics. However, the mechanism underlying the actin filament regulation of reproductive development in passion fruit remains poorly understood. Here, we characterized a villin isovariant in passion fruit, Passiflora edulis VLN4 (PeVLN4), highly and preferentially expressed in pollen. Subcellular localization analysis showed that PeVLN4 decorated distinct filamentous structures in pollen tubes. We next introduced PeVLN4 into Arabidopsis villin mutants to explore its functions on the growing pollen tubes. PeVLN4 rescued defects in the elongation of villin mutant pollen tubes. Pollen tubes expressing PeVLN4 were revealed to be less sensitive to latrunculin B, and PeVLN4 partially rescued defects in the actin filament organization of villin mutant pollen tubes. Additionally, biochemical assays revealed that PeVLN4 bundles actin filaments in vitro. Thus, PeVLN4 is an important regulator of F-actin stability and is required for normal pollen tube growth in passion fruit. This study provides a new insight into the function of the actin regulator villin involved in the reproduction development of passion fruit.
Keyword ：
actin filament actin filament Passiflora edulis Passiflora edulis reproduction development reproduction development VLN gene VLN gene
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| GB/T 7714 | Yang, Hanbing , Wei, Xiuqing , Wang, Lifeng et al. Functional Characterization of PeVLN4 Involved in Regulating Pollen Tube Growth from Passion Fruit [J]. | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES , 2025 , 26 (5) . |
| MLA | Yang, Hanbing et al. "Functional Characterization of PeVLN4 Involved in Regulating Pollen Tube Growth from Passion Fruit" . | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 26 . 5 (2025) . |
| APA | Yang, Hanbing , Wei, Xiuqing , Wang, Lifeng , Zheng, Ping , Li, Junzhang , Zou, Yutong et al. Functional Characterization of PeVLN4 Involved in Regulating Pollen Tube Growth from Passion Fruit . | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES , 2025 , 26 (5) . |
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Key messageTwenty-nine GRAS genes were identified in passion fruit, the N-terminal regions and 3D (three-dimensional) structures were closely related with their tissue-specific expression patterns. Candidate PeGRASs for enhancing stress resistance were identified.AbstractPassion fruit (Passiflora edulis Sims) is a tropical fruit crop with significant edible and ornamental value, but its growth and development are highly sensitive to environmental conditions. The plant-specific GRAS gene family plays critical roles in regulating growth, development, and stress responses. Here, we performed the first comprehensive analysis of the GRAS gene family in passion fruit. A total of 29 GRAS genes were identified and named PeGRAS1 to PeGRAS29 based on their chromosomal locations. Phylogenetic analysis using GRAS proteins from passion fruit, Arabidopsis, and rice revealed that PeGRAS proteins could be classified into 10 subfamilies. Compared to Arabidopsis, passion fruit lacked members from the LAS subfamily but gained one GRAS member (PeGRAS9) clustered with the rice-specific Os4 subfamily. Structural analysis performed in silico revealed that most PeGRAS members were intron less and exhibited conserved motif patterns near the C-terminus, while the N-terminal regions varied in sequence length and composition. Members within certain subfamilies including DLT, PAT1, and LISCL with similar unstructured N-terminal regions and 3D structures, exhibited similar tissue-specific expression patterns. While PeGRAS members with difference in these structural features, even within the same subfamily (e.g., DELLA), displayed distinct expression patterns. These findings highlighted that the N-terminal regions of GRAS proteins may be critical for their specific functions. Moreover, many PeGRAS members, particularly those from the PAT1 subfamily, were widely involved in stress responses, with PeGRAS19 and PeGRAS26 likely playing roles in cold tolerance, and PeGRAS25 and PeGRAS28 in drought resistance. This study provides a foundation for further functional research on PeGRASs and offers potential candidates for molecular breeding aimed at enhancing stress tolerance in passion fruit.
Keyword ：
GRAS gene family GRAS gene family Molecular breeding Molecular breeding Passion fruit Passion fruit Plant development Plant development Stress response Stress response Transcriptional regulation Transcriptional regulation
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| GB/T 7714 | Cai, Xinkai , Li, Denglin , Liu, Chaojia et al. Identification and characterization of GRAS genes in passion fruit (Passiflora edulis Sims) revealed their roles in development regulation and stress response [J]. | PLANT CELL REPORTS , 2025 , 44 (2) . |
| MLA | Cai, Xinkai et al. "Identification and characterization of GRAS genes in passion fruit (Passiflora edulis Sims) revealed their roles in development regulation and stress response" . | PLANT CELL REPORTS 44 . 2 (2025) . |
| APA | Cai, Xinkai , Li, Denglin , Liu, Chaojia , Chen, Jiayi , Wei, Xiuqing , Hu, Sitong et al. Identification and characterization of GRAS genes in passion fruit (Passiflora edulis Sims) revealed their roles in development regulation and stress response . | PLANT CELL REPORTS , 2025 , 44 (2) . |
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How do growth hormones interact to specify female-germline cell types in flowering plants and control production of the first female-germline cell? Here, we find that gibberellin (GA) biosynthesis and signaling are restricted in ovule primordia, with overexpression of receptors and biosynthetic enzymes resulting in multiple and enlarged megaspore mother cells (MMCs) in Arabidopsis. GA signaling machinery interacts with and promotes the degradation of cytokinin (CK) type-B Arabidopsis response regulators (ARR1/10/12), which also directly interact with DELLA proteins. CK biosynthesis and signaling components are expressed in both MMCs and sporophytic cells, with signaling negatively controlled by GA in ovule primordia, and perturbations leading to the induction of multiple, enlarged MMC-like cells. The vacuolar sorting protein SHRUBBY (SHBY) interacts with GA and CK signaling components to block GA-induced degradation. CK signaling restricts multiple sub-epidermal cells in distal ovule primordia from acquiring MMC identity. By balancing degradation activity, GA and CK signaling antagonistically control female-germline cell specification.
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| GB/T 7714 | Cai, Hanyang , Liu, Kaichuang , Ma, Suzhuo et al. Gibberellin and cytokinin signaling antagonistically control female-germline cell specification in Arabidopsis [J]. | DEVELOPMENTAL CELL , 2025 , 60 (5) : 706-722 . |
| MLA | Cai, Hanyang et al. "Gibberellin and cytokinin signaling antagonistically control female-germline cell specification in Arabidopsis" . | DEVELOPMENTAL CELL 60 . 5 (2025) : 706-722 . |
| APA | Cai, Hanyang , Liu, Kaichuang , Ma, Suzhuo , Su, Han , Yang, Jiahong , Sun, Ling et al. Gibberellin and cytokinin signaling antagonistically control female-germline cell specification in Arabidopsis . | DEVELOPMENTAL CELL , 2025 , 60 (5) , 706-722 . |
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Clinacanthus nutans is a valuable traditional medicinal plant that contains enriched active compounds such as triterpenoids and flavonoids. Understanding the accuulation process of these secondary metabolites in C. nutans requires exploring gene expression regulation under abiotic stresses and hormonal stimuli. qRT-PCR is a powerful method for gene expression analysis, with the selection of suitable reference genes being paramount. However, reports on stably expressed reference genes in C. nutans and even across the entire family Acanthaceae are limited. In this study, we evaluated the expression stability of 12 candidate reference genes (CnUBQ, CnRPL, CnRPS, CnPTB1, CnTIP41, CnACT, CnUBC, CnGAPDH, Cn18S, CnCYP, CnEF1 alpha, and CnTUB) in C. nutans across different tissues and under abiotic stresses and MeJA treatment using three programs (geNorm, NormFinder, and BestKeeper). The integrated ranking results indicated that CnUBC, CnRPL, and CnCYP were the most stably expressed genes across different tissues. Under abiotic stress conditions, CnUBC, CnRPL, and CnEF1 alpha were the most stable, while under MeJA treatment, CnRPL, CnEF1 alpha, and CnGAPDH exhibited the highest stability. Additionally, CnRPL, CnUBC, and CnEF1 alpha were the most stable reference genes across all tested samples, whereas CnGAPDH was the least stable. CnRPL, consistently ranking among the top three most stable genes, may therefore serve as an ideal reference gene for qRT-PCR analysis in C. nutans. To further validate the selected reference genes, we assessed the expression of two key biosynthetic genes, CnPAL and CnHMGR. The results confirmed that using the most stable reference genes yielded expression patterns consistent with biological expectations, while using unstable reference genes led to significant deviations. These findings offer valuable insights for accurately quantifying target genes via qRT-PCR in C. nutans, facilitating investigations into the mechanisms underlying active compound accumulation.
Keyword ：
Clinacanthus nutans Clinacanthus nutans molecular biology techniques molecular biology techniques plant stress response plant stress response quantitative real-time PCR quantitative real-time PCR reference genes reference genes
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| GB/T 7714 | An, Chang , Lu, Lin , Yao, Yixin et al. Selection and Validation of Reference Genes in Clinacanthus nutans Under Abiotic Stresses, MeJA Treatment, and in Different Tissues [J]. | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES , 2025 , 26 (6) . |
| MLA | An, Chang et al. "Selection and Validation of Reference Genes in Clinacanthus nutans Under Abiotic Stresses, MeJA Treatment, and in Different Tissues" . | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 26 . 6 (2025) . |
| APA | An, Chang , Lu, Lin , Yao, Yixin , Liu, Ruoyu , Cheng, Yan , Lin, Yanxiang et al. Selection and Validation of Reference Genes in Clinacanthus nutans Under Abiotic Stresses, MeJA Treatment, and in Different Tissues . | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES , 2025 , 26 (6) . |
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Selenium-enriched Dendrobium huoshanense C. Z. Tang et S. J. Cheng is a precious medicinal herb that combines traditional therapeutic value with modern nutritional benefits. However, its wild populations primarily inhabit special habitats like cliffs and rock crevices, resulting in limited yield and low selenium content. This study optimized an in vitro selenium-enriched cultivation system for D. huoshanense, investigating the regulatory mechanisms of selenium on physiological metabolism by modulating exogenous selenium concentrations, and determining the spatiotemporal distribution and speciation of selenium in plantlets. The results showed the optimal medium composition was as follows: MS + IBA (0.1 mg/L) + NAA (0.6 mg/L) + 7% agar + 30% sucrose + 100 g/L banana homogenate + 3 mg/L sodium selenite (pH 5.8). Under these conditions, roots served as the primary selenium accumulation sites at 30 and 60 days of cultivation. After 90 days, selenium redistribution occurred from storage organs (roots) to metabolically active organs (leaves). Organic selenium constituted 83.70% of total selenium, comprising 44.90% selenoproteins, 29.20% selenopolysaccharides, and 9.60% other organic forms. The contents of selenomethionine (SeMet), methylselenocysteine (MeSeCys), and selenocysteine (SeCys2) were 0.63 +/- 0.04, 0.20 +/- 0.11, and 0.28 +/- 0.06 mg/kg, respectively. Using plant tissue culture technology, we successfully cultivated selenium-enriched D. huoshanense, and investigated its growth metabolism, selenium translocation mechanisms, and selenium speciation. These findings provide theoretical foundations for developing selenium-enriched medicinal materials and have significant implications for enhancing the medicinal value of D. huoshanense.
Keyword ：
Dendrobium huoshanense Dendrobium huoshanense selenium speciation analysis selenium speciation analysis selenoamino acid selenoamino acid tissue culture tissue culture
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| GB/T 7714 | Wu, Yulai , An, Chang , Wang, Yanjie et al. Dendrobium huoshanense In Vitro Culture and Selenium Metabolism: Speciation Mechanisms [J]. | HORTICULTURAE , 2025 , 11 (10) . |
| MLA | Wu, Yulai et al. "Dendrobium huoshanense In Vitro Culture and Selenium Metabolism: Speciation Mechanisms" . | HORTICULTURAE 11 . 10 (2025) . |
| APA | Wu, Yulai , An, Chang , Wang, Yanjie , Sun, Yuqi , Liu, Changbin , Wang, Bingrui et al. Dendrobium huoshanense In Vitro Culture and Selenium Metabolism: Speciation Mechanisms . | HORTICULTURAE , 2025 , 11 (10) . |
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BackgroundPineapple (Ananas comosus L.), an economically significant tropical fruit crop, is highly susceptible to low-temperature during cultivation. The abscisic acid (ABA) and stress-inducible HVA22 gene family is known to play important roles in growth regulation and abiotic stress response, but its functions in pineapple remain unclear.ResultsWe identified 11 HVA22 genes (AcHVA22A - AcHVA22K) in the pineapple genome and grouped them into four phylogenetic clades. Expression analysis showed that most AcHVA22 genes displayed tissue- or developmental stage-specific expression patterns, suggesting their diverse functions in pineapple growth and development. Subcellular localization analyses revealed diverse localizations of AcHVA22 proteins, including plasma membrane, cytoplasm, and nucleus. And regulatory predictions indicated control by multiple cis-elements, transcription factors, and miRNAs, which may contribute to their functional diversification. Most AcHVA22 genes responded consistently to ABA, GA, and drought treatments, but their responses to high and low-temperatures varied. Notably, AcHVA22C/D/E/G/I/K were up-regulated under both short- and long-term cold treatments in two different pineapple varieties, highlighting their potential key roles in cold stress tolerance.ConclusionsThis study provided the first genome-wide characterization of the HVA22 gene family in pineapple. The identification of candidate genes involved in cold stress response offers new insights into HVA22 functions in tropical fruits and provides valuable resources for improving cold resistance in pineapple breeding.
Keyword ：
Cold response Cold response Expression profiling Expression profiling HVA22 genes HVA22 genes Pineapple Pineapple Tissue-specific Tissue-specific
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| GB/T 7714 | Hou, Zhimin , Cai, Xinkai , Wu, Jiahao et al. Identification and characterization of HVA22 genes in pineapple (Ananas comosus L.) revealed their potential roles in development regulation and cold stress response [J]. | BMC PLANT BIOLOGY , 2025 , 25 (1) . |
| MLA | Hou, Zhimin et al. "Identification and characterization of HVA22 genes in pineapple (Ananas comosus L.) revealed their potential roles in development regulation and cold stress response" . | BMC PLANT BIOLOGY 25 . 1 (2025) . |
| APA | Hou, Zhimin , Cai, Xinkai , Wu, Jiahao , Lu, Lin , Liu, Chaojia , Zhang, Yangmei et al. Identification and characterization of HVA22 genes in pineapple (Ananas comosus L.) revealed their potential roles in development regulation and cold stress response . | BMC PLANT BIOLOGY , 2025 , 25 (1) . |
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