Query：
学者姓名：傅俊生
Refining：
Year
Type
Indexed by
Source
Complex
Co-Author
Language
Clean All
Abstract ：
【目的】对采自西藏地区的野生菌株X21020进行鉴定,探究其最佳培养条件及驯化栽培条件,并测定其驯化栽培子实体的常规营养成分,为血红密孔菌的进一步开发利用提供参考。【方法】采用组织分离法对野生菌株X21020进行分离纯化,采用分子测序法和形态学特征观察法对其进行菌种鉴定,探究其生物学特性并开展驯化栽培,测定驯化栽培成功子实体的常规营养成分。【结果】结合形态学特征观察和ITS测序,将野生菌株X21020鉴定为血红密孔菌(Fabisporus sanguineus)。该菌株菌丝生长的最适碳源为葡萄糖,最适氮源为蛋白胨,p H为5,最适培养温度为40℃。该菌株在以杂木屑为主的栽培料中生长良好,菌丝满袋时间为30 d,原基形成时间为10 d,80 d后采收第一潮菇,单朵子实体鲜质量为32.82 g。该菌株子实体中膳食纤维含量高达7.110 g/kg,粗蛋白含量达1.190 g/kg,脂肪含量仅为0.020 g/kg。含有15种氨基酸,氨基酸总含量为0.486 g/kg。其中药效氨基酸含量为0.109 g/kg,占氨基酸总含量的22.43%,且以天冬氨酸含量最高(0.061 g/kg)。【结论】西藏来源的血红密孔菌X21020可在以杂木屑为主的栽培料中良好生长,其驯化子实体含有丰富的蛋白质和膳食纤维,氨基酸种类多,脂肪含量低,该结果为其在医学和保健品领域的开发利用提供了参考。
Keyword ：
生物学特性 生物学特性 菌种鉴定 菌种鉴定 营养评价 营养评价 血红密孔菌 血红密孔菌 食用菌 食用菌 驯化栽培 驯化栽培
Cite：
Copy from the list or Export to your reference management。
| GB/T 7714 | 张君丽 , 傅俊生 . 西藏血红密孔菌的驯化栽培及营养评价 [J]. | 西北农林科技大学学报(自然科学版) , 2026 , (01) : 148-156 . |
| MLA | 张君丽 等. "西藏血红密孔菌的驯化栽培及营养评价" . | 西北农林科技大学学报(自然科学版) 01 (2026) : 148-156 . |
| APA | 张君丽 , 傅俊生 . 西藏血红密孔菌的驯化栽培及营养评价 . | 西北农林科技大学学报(自然科学版) , 2026 , (01) , 148-156 . |
| Export to | NoteExpress RIS BibTex |
Version ：
Abstract ：
粗毛纤孔菌是一种天然的药用真菌,具有多种生物活性,其子实体颜色在成熟后期可由黄褐色转变为黑色,即为黑色素。通过探究其理化性质发现,粗毛纤孔菌黑色素(IHFM)属于真黑色素,具有良好的热、光和及金属稳定性等。黑色素是粗毛纤孔菌的活性成分之一,具有保肝、抗癌、调节肠道菌群结构、抗氧化等活性。首先,在保肝方面的研究显示,IHFM一方面通过抑制Keap-1、激活Nrf2信号通路显著提高下游GCLC、HO-1、NQO1等的表达;另一方面显著抑制MyD88介导的TLR4/NF-κβ信号通路并降低下游炎症因子IL-1β、TNF-α、IL-6等的表达水平,进而缓解小鼠酒精性引发的肝损伤。在调节肠道菌群方面,IHFM可以调节酒精性肝损伤小鼠中肠道菌群的结构,上调肠道菌群中有益菌的相对丰度,降低有害菌的相对丰度。综上,我们的研究可为IHFM开发为保肝、抗氧化剂等相关产品奠定理论基础。
Keyword ：
Nrf2/Keap-1信号通路 Nrf2/Keap-1信号通路 保肝作用 保肝作用 肠道菌群 肠道菌群 黑色素 黑色素
Cite：
Copy from the list or Export to your reference management。
| GB/T 7714 | 李晓敏 , 袁源 , 吴小平 et al. 粗毛纤孔菌黑色素的生物学活性研究 [C] //中国菌物学会2025年学术年会 . 2025 . |
| MLA | 李晓敏 et al. "粗毛纤孔菌黑色素的生物学活性研究" 中国菌物学会2025年学术年会 . (2025) . |
| APA | 李晓敏 , 袁源 , 吴小平 , 傅俊生 . 粗毛纤孔菌黑色素的生物学活性研究 中国菌物学会2025年学术年会 . (2025) . |
| Export to | NoteExpress RIS BibTex |
Version ：
Abstract ：
本发明为一种粗毛纤孔菌黑色素的制备方法和应用，粗毛纤孔菌黑色素的制备方法，包括如下步骤：S1，收集粗毛纤孔菌子实体并烘干粉碎后经筛网筛选过滤；S2，称取粉末并加入NaOH溶液，并置于超声波中充分反应；S3，在离心机上经离心处理后，收集上清液；S4，在S3收集的上清液中加入酸性溶液并加热；S5，再次离心后收集沉淀物；S6，将沉淀物进行纯化；S7，离心收集上清液，最终通过流水透析后，冷冻干燥后获得纯化后的粗毛纤孔菌子实体黑色素样品。所述粗毛纤孔菌黑色素能够显著抑制人乳腺癌细胞生长、增殖、迁移的能力；并诱导人乳腺癌细胞的凋亡和人乳腺癌细胞焦亡。因此本发明粗毛纤孔菌黑色素具有开发为抗乳腺癌药物的潜能。
Cite：
Copy from the list or Export to your reference management。
| GB/T 7714 | 傅俊生 , 李晓敏 , 吴小平 . 粗毛纤孔菌黑色素的制备方法和应用 : CN202510086857.8[P]. | 2025-01-20 . |
| MLA | 傅俊生 et al. "粗毛纤孔菌黑色素的制备方法和应用" : CN202510086857.8. | 2025-01-20 . |
| APA | 傅俊生 , 李晓敏 , 吴小平 . 粗毛纤孔菌黑色素的制备方法和应用 : CN202510086857.8. | 2025-01-20 . |
| Export to | NoteExpress RIS BibTex |
Version ：
Abstract ：
The selection and breeding of high-quality wild edible fungal strains can bring significant economic and social benefits. A wild fungal strain (X21185) from the Tibetan Plateau was isolated and identified as a novel Pleurotus pulmonarius (P. pulmonarius) based on its morphological and molecular characteristics. The appropriate culture conditions for P. pulmonarius were determined. The nutrient contents of P. pulmonarius fruiting bodies were analyzed. Compared with the conventional nutritional contents of the representative edible fungi (Pleurotus ostreatu and Pleurotus eryngii) and egg, the protein, ash, and dietary fiber contents of P. pulmonarius were higher. Four types of essential amino acids, seven types of nonessential amino acids, the total essential and nonessential amino acids of P. pulmonarius were present in considerably higher quantities than those of representative edible fungi (Pleurotus ostreatus and Pleurotus citrinopileatus) and egg, respectively. P. pulmonarius polysaccharides (PPPs) had strong ABTS+, DPPH, and hydroxyl free radical scavenging activities (EC50: 0.051, 3.322, and 2.87 mg/mL, respectively), and the cytotoxicity was higher against HepG2 hepatocellular carcinoma cells (IC50: 1.501 mg/mL) than against MDA-MB-468 triple-negative breast cancer cells (IC50: 2.183 mg/mL). This study provides a foundation for the development of the novel wild P. pulmonarius strain.
Keyword ：
antioxidant activity antioxidant activity cytotoxicity cytotoxicity fungi identification fungi identification nutrient composition nutrient composition Pleurotus pulmonarius Pleurotus pulmonarius
Cite：
Copy from the list or Export to your reference management。
| GB/T 7714 | Jiang, Hao , Gao, Lei , Hu, Xin et al. Identification and Nutrient Composition of a Wild Pleurotus pulmonarius Strain from Tibet, and the Antioxidant and Cytotoxic Activities of Polysaccharides from This Fungus [J]. | FOODS , 2025 , 14 (7) . |
| MLA | Jiang, Hao et al. "Identification and Nutrient Composition of a Wild Pleurotus pulmonarius Strain from Tibet, and the Antioxidant and Cytotoxic Activities of Polysaccharides from This Fungus" . | FOODS 14 . 7 (2025) . |
| APA | Jiang, Hao , Gao, Lei , Hu, Xin , Fu, Junsheng , Zhang, Junli . Identification and Nutrient Composition of a Wild Pleurotus pulmonarius Strain from Tibet, and the Antioxidant and Cytotoxic Activities of Polysaccharides from This Fungus . | FOODS , 2025 , 14 (7) . |
| Export to | NoteExpress RIS BibTex |
Version ：
Abstract ：
本发明公开了一种杏鲍菇菌株及其应用。本发明中，福杏鲍217是针对瓶式栽培选育，杏鲍菇菌种根据基因组测序数据，采用Kmer差减组装技术对‘福杏鲍217’、亲本‘日引1号’全基因组测序数据进行两两互比，获得特异性差异片段序列(SV)，获得差异片段(SV)，随机选取4个SV序列设计特异性引物4对，对杏鲍基‘福杏鲍217’和亲本‘日引1号’进行扩增，本发明的杏鲍菇菌种‘福杏鲍217’针对瓶式栽培选育，以杏鲍菇‘日引1号’为亲本通过自交杂交选育而得，杏鲍菇菌种保藏编号为CCTCC M 20242462，保藏地址为湖北省武汉市武昌区八一路299号。‘福杏鲍217’菌盖为球面型、浅灰色，菌柄颜色为白色，栽培周期较短，产量高，耐贮藏、耐运输，有很好的开发应用前景。
Cite：
Copy from the list or Export to your reference management。
| GB/T 7714 | 刘新锐 , 陈炳智 , 邓优锦 et al. 一种杏鲍菇菌株及其应用 : CN202411624224.X[P]. | 2024-11-14 . |
| MLA | 刘新锐 et al. "一种杏鲍菇菌株及其应用" : CN202411624224.X. | 2024-11-14 . |
| APA | 刘新锐 , 陈炳智 , 邓优锦 , 江玉姬 , 傅俊生 , 岑光红 et al. 一种杏鲍菇菌株及其应用 : CN202411624224.X. | 2024-11-14 . |
| Export to | NoteExpress RIS BibTex |
Version ：
Abstract ：
本发明提供了一种浅色且高产的杏鲍菇菌株，属于农业微生物领域。本发明的杏鲍菇菌株为刺芹侧耳（Pleurotus eryngii）福杏鲍64，已于2024年11月7日保藏于中国典型培养物保藏中心，保藏编号为CCTCC NO：M 20242460，保藏地址为湖北省武汉市武昌区八一路299号。‘福杏鲍64’菌盖凹面型、浅灰色，菌柄颜色洁白，有很好的开发应用前景。
Cite：
Copy from the list or Export to your reference management。
| GB/T 7714 | 刘新锐 , 陈炳智 , 邓优锦 et al. 一种浅色且高产的杏鲍菇菌株 : CN202411648008.9[P]. | 2024-11-18 . |
| MLA | 刘新锐 et al. "一种浅色且高产的杏鲍菇菌株" : CN202411648008.9. | 2024-11-18 . |
| APA | 刘新锐 , 陈炳智 , 邓优锦 , 刘芳 , 傅俊生 , 江玉姬 et al. 一种浅色且高产的杏鲍菇菌株 : CN202411648008.9. | 2024-11-18 . |
| Export to | NoteExpress RIS BibTex |
Version ：
Abstract ：
Natural melanin biopolymers exhibit a variety of biological activities, but their commercial development is constrained by numerous factors, including high costs, unsustainable sources, the use of harmful solvents during extraction, and low extraction efficiency. Notably, existing research indicates that synthetic melanin differs from natural melanin in nature, and this difference may directly impact its application efficacy. Additionally, the extraction process itself is highly challenging, primarily due to the diversity and complexity of melanin biopolymer structures. The melanin produced by edible fungi primarily belongs to the eumelanin category. Given its outstanding sustainability and accessibility, it is regarded as an ideal raw material for industrial production. To deepen our understanding of edible fungus-derived melanin and promote its effective application across various fields, a comprehensive review of research on melanin isolated from edible fungi is urgently needed. Such a review will help researchers from different disciplinary backgrounds recognize the importance of edible fungus melanin and provide reference information for their research planning. With this objective in mind, this report reviews the latest research progress in recent years regarding extraction methods, structural characterization, biological activity, and application areas of edible fungus-derived melanin. Additionally, the report explores key characteristic parameters for distinguishing different types of melanin and emphasizes the importance of deepening our understanding of the biosynthetic mechanisms of edible mushroom melanin, aiming to lay the foundation for its efficient production and application in the future.
Keyword ：
applications applications biological activity biological activity characterization characterization edible fungi melanin edible fungi melanin eumelanin eumelanin extraction extraction
Cite：
Copy from the list or Export to your reference management。
| GB/T 7714 | Tang, Jiandong , Shen, Hebin , Lv, Wenyu et al. Edible Fungi Melanin: Recent Advances in Extraction, Characterization, Biological Activity and Applications [J]. | JOURNAL OF FUNGI , 2025 , 11 (10) . |
| MLA | Tang, Jiandong et al. "Edible Fungi Melanin: Recent Advances in Extraction, Characterization, Biological Activity and Applications" . | JOURNAL OF FUNGI 11 . 10 (2025) . |
| APA | Tang, Jiandong , Shen, Hebin , Lv, Wenyu , Zhang, Jingxuan , Fu, Junsheng . Edible Fungi Melanin: Recent Advances in Extraction, Characterization, Biological Activity and Applications . | JOURNAL OF FUNGI , 2025 , 11 (10) . |
| Export to | NoteExpress RIS BibTex |
Version ：
Abstract ：
To enhance the systematicity and integrity of mushroom resource research by supplementing the mushroom germplasm bank through the collection of wild mushroom specimens, this project focused on a wild mushroom strain collected from Tibet Autonomous Region. The strain was identified and characterized through morphological observation, ITS sequencing, biological trait analysis, and cultivation experiments. The results indicated that the strain belongs to Pleurotus placentodes. The optimal growth conditions for this strain were determined as follows: fructose and sucrose as the preferred carbon source, yeast powder and beef powder as the optimal nitrogen source, pH 5.0, and a temperature of 20 degrees C. In cultivation experiments, the mycelium fully colonized the substrate in 23 days, primordia formation occurred within 10 d, and the fruiting period lasted 7 d, with an average fresh weight of 39.40 g per fruiting body. Nutritional analysis revealed that P. placentodes has a high protein content (30.30%), high soluble sugar content (6.60%), low fat content (1.00%), and a rich profile of essential amino acids (7.96%). This study successfully isolated, identified, and conducted domestication cultivation and nutritional analysis of a wild P. placentodes, providing scientific references for its potential industrial-scale production.
Keyword ：
cultivation cultivation domestication domestication nutritional value nutritional value Pleurotus placentodes Pleurotus placentodes wild mushroom wild mushroom
Cite：
Copy from the list or Export to your reference management。
| GB/T 7714 | Cai, Linna , Li, Xiaomin , Hu, Xin et al. Characteristics, Domestication and Nutrient Analysis of Wild Pleurotus placentodes [J]. | FOODS , 2025 , 14 (22) . |
| MLA | Cai, Linna et al. "Characteristics, Domestication and Nutrient Analysis of Wild Pleurotus placentodes" . | FOODS 14 . 22 (2025) . |
| APA | Cai, Linna , Li, Xiaomin , Hu, Xin , Zhang, Junli , Sun, Huijuan , Gao, Lei et al. Characteristics, Domestication and Nutrient Analysis of Wild Pleurotus placentodes . | FOODS , 2025 , 14 (22) . |
| Export to | NoteExpress RIS BibTex |
Version ：
Abstract ：
Hericium coralloides is a valuable medicinal and edible mushroom renowned for its unique bioactive compounds. This study focuses on the isolation of a wild strain (SH001) exhibiting promising cultivation potential and health promoting properties. A wild fungal strain from the Tibetan Plateau was isolated and identified as a novel H. coralloides based on its morphological and molecular characteristics. The optimal growth conditions were found to be 30 degrees C, pH 7.0, fructose as the preferred carbon source, and yeast extract as the optimal nitrogen source. Nutritional analysis revealed that the fruiting bodies were rich in protein (15.4 g/100 g dry weight), dietary fiber (34.7 g/100 g dry weight), and minerals, while being low in fat (3.5 g/100 g dry weight). The most abundant amino acids were glutamic acid, followed by aspartic acid. The polysaccharides exhibited significant antioxidant activity, with ABTS+ scavenging comparable to that of Vitamin C (Vc), achieving a clearance rate of 96.95% at concentrations between 0.25-5.00 mg/mL. At a concentration of 5 mg/mL, the DPPH and OH radical scavenging activities reached their peak (83.77% and 67.31%, respectively), along with the highest iron ion reducing capacity (FRAP value: 4.43 mmol/L. Polysaccharides also exhibited notable anticancer activity, inhibiting HepG2 liver cancer cells and MDA-MB-468 breast cancer cells, with IC50 values of 3.896 mg/mL and 2.561 mg/mL, respectively. This study demonstrates that wild H. coralloides can be successfully cultivated in vitro. In conclusion, the fruiting bodies possess substantial nutritional value, and the polysaccharides extracted from them show promising antioxidant and anticancer activities, particularly against HepG2 liver cancer cells and MDA-MB-468 breast cancer cells.
Keyword ：
antioxidant properties antioxidant properties biological characteristics biological characteristics culture conditions culture conditions domestication and cultivation domestication and cultivation Hericium coralloides Hericium coralloides nutritional composition nutritional composition
Cite：
Copy from the list or Export to your reference management。
| GB/T 7714 | Li, Yun , Cai, Jiarong , Li, Xiaomin et al. Domestication Cultivation and Nutritional Analysis of Hericium coralloides [J]. | JOURNAL OF FUNGI , 2025 , 11 (11) . |
| MLA | Li, Yun et al. "Domestication Cultivation and Nutritional Analysis of Hericium coralloides" . | JOURNAL OF FUNGI 11 . 11 (2025) . |
| APA | Li, Yun , Cai, Jiarong , Li, Xiaomin , Hu, Xin , Zhang, Junli , Wu, Xiaoping et al. Domestication Cultivation and Nutritional Analysis of Hericium coralloides . | JOURNAL OF FUNGI , 2025 , 11 (11) . |
| Export to | NoteExpress RIS BibTex |
Version ：
Abstract ：
A fungal strain (X21156) collected in Tibet was used as the material, identified based on its morphological characteristics and internal transcribed spacer (ITS) sequence; its optimal culture conditions were analyzed by single-factor experiments; artificial domestication and cultivation were carried out; its nutrient composition was determined; and the bioactivities of its polysaccharides were detected using chemical antioxidant assays and MTT assays. The results showed that the strain was Pleurotus citrinopileatus Sing. Its optimal culture conditions were a pH of 7, a temperature of 25 degrees C, glucose (20 gL-1) as the carbon source, and yeast powder (20 gL-1) as the nitrogen source. The fresh weight of a single domesticated fruiting body was 41.16 g. The strain had high protein (28.5%), high fiber (34%), and low fat (1.4%) contents, with high proportions of fresh and sweet amino acids. Polysaccharides had good scavenging ability on ABTS+, DPPH, and OH free radicals (EC50 0.06 mg/mL, 1.21 mg/mL, and 3.62 mg/mL, respectively), and the cytotoxicity of polysaccharides to hepatocellular carcinoma cells (HepG2) (IC50: 1.69 mg/mL) was higher than that of triple-negative breast cancer cells (MDA-MB-468) (IC50: 1.76 mg/mL). In conclusion, the study provides a reference on the optimal culture conditions, domestication and cultivation, and dietary and medicinal values of wild P. citrinopileatus Sing.
Keyword ：
antioxidant activities antioxidant activities biological properties biological properties cytotoxic effect cytotoxic effect fungi identification fungi identification nutritional composition nutritional composition Pleurotus citrinopileatus Pleurotus citrinopileatus
Cite：
Copy from the list or Export to your reference management。
| GB/T 7714 | Xiao, Xiaoshan , Li, Yun , Li, Xiaomin et al. Identification, Nutrient Composition, and Evaluation of a Wild Pleurotus citrinopileatus Strain (X21156) from Tibet for Antioxidant and Cytotoxic Activities [J]. | HORTICULTURAE , 2024 , 10 (4) . |
| MLA | Xiao, Xiaoshan et al. "Identification, Nutrient Composition, and Evaluation of a Wild Pleurotus citrinopileatus Strain (X21156) from Tibet for Antioxidant and Cytotoxic Activities" . | HORTICULTURAE 10 . 4 (2024) . |
| APA | Xiao, Xiaoshan , Li, Yun , Li, Xiaomin , Hu, Xin , Zhang, Junli , Wu, Xiaoping et al. Identification, Nutrient Composition, and Evaluation of a Wild Pleurotus citrinopileatus Strain (X21156) from Tibet for Antioxidant and Cytotoxic Activities . | HORTICULTURAE , 2024 , 10 (4) . |
| Export to | NoteExpress RIS BibTex |
Version ：
Export
| Results： |
Selected to |
| Format： |
