Query：
学者姓名：缪颖
Refining：
Year
Type
Indexed by
Source
Complex
Co-Author
Language
Clean All
Abstract ：
本发明涉及植物种子种植技术领域，具体涉及一种水稻种子解除休眠的方法，具体包括以下步骤：1)种子选择：选取4℃种窖贮存的处于休眠状态的水稻种子；2)将步骤1)选取的水稻种子在促解除休眠药剂中浸泡24小时后，用清水洗净；3)在28℃恒温下进行标准发芽实验，观察记载发芽情况。本发明优点在于：步骤设计合理，便于操作，针对水稻种子解除休眠的效果显著。可以方便地应用于实际生产中，提高种子的利用率和种植效益。
Cite：
Copy from the list or Export to your reference management。
| GB/T 7714 | 汪小力 , 王玮琦 , 郑祥梓 et al. 一种水稻种子解除休眠的方法 : CN202411843420.6[P]. | 2024-12-14 . |
| MLA | 汪小力 et al. "一种水稻种子解除休眠的方法" : CN202411843420.6. | 2024-12-14 . |
| APA | 汪小力 , 王玮琦 , 郑祥梓 , 缪颖 . 一种水稻种子解除休眠的方法 : CN202411843420.6. | 2024-12-14 . |
| Export to | NoteExpress RIS BibTex |
Version ：
Abstract ：
本发明涉及水仙花原生质体技术领域，具体涉及一种水仙花原生质体分离与转化体系建立方法，具体包括以下步骤：配置10mL buffer；避光抽真空30min；取出，用枪头吸除buffer；避光抽真空30min；室温酶解消化；释放原生质体；洗涤原生质体并终止酶解，离心，弃上清，留沉淀；分层；离心，分为3层，原生质体转移；用另一只离心管平衡并离心，弃上清，留沉淀，加W5至5mL轻轻晃动悬浮原生质体；镜检；使原生质体重悬复苏，离心；弃上清，留沉淀，原生质体的转化体系建立；本发明优点在于：高效、简便地针对水仙花原生质体分离与转化体系建立，为水仙花的遗传改良和生物技术研究提供有力的工具。
Cite：
Copy from the list or Export to your reference management。
| GB/T 7714 | 汪小力 , 吴卓豫 , 杨静文 et al. 一种水仙花原生质体分离与转化体系建立方法 : CN202411843426.3[P]. | 2024-12-14 . |
| MLA | 汪小力 et al. "一种水仙花原生质体分离与转化体系建立方法" : CN202411843426.3. | 2024-12-14 . |
| APA | 汪小力 , 吴卓豫 , 杨静文 , 郑祥梓 , 缪颖 . 一种水仙花原生质体分离与转化体系建立方法 : CN202411843426.3. | 2024-12-14 . |
| Export to | NoteExpress RIS BibTex |
Version ：
Abstract ：
Leaf senescence triggers major metabolic changes that recycle resources and ensure plant survival and is sensitive to environmental conditions. In this study, we characterized OsS40-14 using CRISPR/Ca9 mutants and overexpression lines to address its function in rice (Oryza sativa) flag leaf senescence. The oss40-14 mutants displayed a stay-green leaf phenotype under dark treatment, with preserved chlorophylls and photosynthetic capacity and reduced reactive oxygen species (ROS) levels. In contrast, OsS40-14 overexpression lines exhibited accelerated leaf senescence. Transcriptome analysis of dark-treated flag leaf samples revealed that the 1,585 differentially expressed genes in oss40-14 vs WT were enriched in macromolecular metabolism, photosynthesis, and stress responses. In addition, tsCUT&Tag-seq analysis using GFP-tagged OsS40-14 revealed 2,311 genomic loci bound by OsS40-14, with approximately 40.95% of the binding signal enriched at transcription start sites. The consensus binding motif of OsS40-14 was identified as TACCCACAAGACAC, with a seed sequence of "ACCCA." Finally, integrated analysis of the transcriptome and tsCUT&Tag-seq data sets revealed that 153 OsS40-14-targeted candidates comprised 66 potentially repressed genes enriched in plastid organization and photosynthetic processes and 41 putatively activated genes associated with stress signaling and senescence. Overall, our results suggest that OsS40-14 fine-tunes the photosynthetic machinery and ROS homeostasis during developmental senescence and under dark conditions, thereby accelerating leaf senescence, reducing photosynthetic assimilation and grain filling in rice. The rice transcription factor S40-14 plays a positive role in dark-induced leaf senescence by regulating chloroplast and ROS-related gene network to adapt the changing environment for plant survival.
Cite：
Copy from the list or Export to your reference management。
| GB/T 7714 | Habiba , Fan, Chunlan , Hong, Wuqiang et al. The rice transcription factor S40-14 regulates chloroplast and reactive oxygen species-related gene networks and promotes dark-induced leaf senescence [J]. | PLANT PHYSIOLOGY , 2025 , 199 (1) . |
| MLA | Habiba et al. "The rice transcription factor S40-14 regulates chloroplast and reactive oxygen species-related gene networks and promotes dark-induced leaf senescence" . | PLANT PHYSIOLOGY 199 . 1 (2025) . |
| APA | Habiba , Fan, Chunlan , Hong, Wuqiang , Wang, Weiqi , Shi, Ximiao , Wang, Xiaowei et al. The rice transcription factor S40-14 regulates chloroplast and reactive oxygen species-related gene networks and promotes dark-induced leaf senescence . | PLANT PHYSIOLOGY , 2025 , 199 (1) . |
| Export to | NoteExpress RIS BibTex |
Version ：
Abstract ：
Plants are continuously challenged by pathogenic threats, including fungi, bacteria, and viruses, across all stages of their growth and development. To combat these, plants have evolved a sophisticated innate immune system, with MAMP-triggered immunity (MTI) serving as an important early defense mechanism. The causative agent of late blight, Phytophthora infestans, secretes effector proteins such as SFI5 that suppress host immune responses, resulting in severe damage. This study explores the role of SFI5 in modulating MTI in host plants. Bioinformatics research revealed a conserved ATP/GTP-binding motif in the N-terminal domain of SFI5, with Lys82 being critical for effector function. Lys82 mutation lowered SFI5 ' s capacity to suppress flg22-triggered reactive oxygen species (ROS) and calcium bursts in tomato protoplasts, but it had no effect on its interaction with calmodulinbinding proteins or kinase activity. In vitro assays confirmed that SFI5 exhibits GTPase activity, unaffected by the Lys82 mutation or the presence of calmodulin. Pull-down assays combined with protein spectrum analysis revealed NbPHB1, a positive regulator of MTI, as a potential binding partner of SFI5. Functional assay demonstrated that NbPHB1 promotes MTI marker gene expression and decreases lesion size in Nicotiana benthamiana, whereas virus-induced gene silencing (VIGS) of NbPHB1 weakens MTI and increases lesion size. SFI5 suppresses NbPHB1 expression, inhibiting MTI and exacerbating lesion development during infection. In conclusion, SFI5 targets the MTI regulator NbPHB1 to inhibit immune responses, with its ATP/GTP-binding motif playing a key role in effector function. These findings provide insights into P. infestans pathogenicity and suggest potential targets for developing resistant crops.
Keyword ：
NbPHB1 NbPHB1 Phytophthora infestans Phytophthora infestans Plant MTI immunity Plant MTI immunity RxLR effector protein SFI5 RxLR effector protein SFI5
Cite：
Copy from the list or Export to your reference management。
| GB/T 7714 | Habiba, Jian , Mu, Jian , Wang, Weiqi et al. RXLR effector SFI5 of Phytophthora infestans suppress MAMP-triggered immunity via inhibition of NbPHB1 in Nicotiana benthamiana [J]. | PLANT STRESS , 2025 , 16 . |
| MLA | Habiba, Jian et al. "RXLR effector SFI5 of Phytophthora infestans suppress MAMP-triggered immunity via inhibition of NbPHB1 in Nicotiana benthamiana" . | PLANT STRESS 16 (2025) . |
| APA | Habiba, Jian , Mu, Jian , Wang, Weiqi , Dou, Zhuoning , Liao, Dongmei , Dai, Huiling et al. RXLR effector SFI5 of Phytophthora infestans suppress MAMP-triggered immunity via inhibition of NbPHB1 in Nicotiana benthamiana . | PLANT STRESS , 2025 , 16 . |
| Export to | NoteExpress RIS BibTex |
Version ：
Abstract ：
WRKY transcription factors play a predominant role in plant stress responses, as well as growth and development. Although WRKY genes have been extensively studied in model plants, little is known about them in Brassica rapa. In this study, the BrWRKY22 gene was isolated and characterized. BrWRKY22 is nuclear localized and has self-activation and dimerization activity. BrWRKY22 was highly expressed in young leaves, roots, and stems. The overexpressed BrWRKY22 Arabidopsis and Brassica rapa lines exhibited a dwarfish, delayed flowering and leaf senescence phenotype compared to the wild-type (WT). Molecular evidence showed that the transcript levels of BrCHLP are increased, whereas those of BrLFY, BrSOC1, BrGA20OX2, BrGA3OX1, and BrGASA6 are significantly decreased in BrWRKY22 overexpressing plants compared to the WT. BrWRKY22 can bind directly to the promoters of BrCHLP and BrGA20OX2, activating BrCHLP and repressing BrGA20OX2 gene transcription. The chlorophyll b and tocopherol levels are increased, whereas the GA and ABA levels are significantly decreased, in three-week-old BrWRKY22 overexpressing Brassica lines compared to the WT. Collectively, our results suggest that BrWRKY22 directly controls chlorophyll b and GA biosynthesis and plays a repressive role in leaf senescence and the initiation of flowering in Brassica rapa plant development.
Keyword ：
Brassica Brassica chlorophyll chlorophyll flowering flowering gibberellin gibberellin senescence senescence WRKY transcription factor WRKY transcription factor
Cite：
Copy from the list or Export to your reference management。
| GB/T 7714 | Uddin, Junaite Bin Gias , Zhuo, Tingzhen , Li, Xiaojie et al. Overexpressing BrWRKY22 Delays Flowering and Leaf Senescence via Inhibition of GA Biosynthesis in Brassica rapa [J]. | PLANTS-BASEL , 2025 , 14 (11) . |
| MLA | Uddin, Junaite Bin Gias et al. "Overexpressing BrWRKY22 Delays Flowering and Leaf Senescence via Inhibition of GA Biosynthesis in Brassica rapa" . | PLANTS-BASEL 14 . 11 (2025) . |
| APA | Uddin, Junaite Bin Gias , Zhuo, Tingzhen , Li, Xiaojie , Wu, Xuan , Wu, Zhuoyu , Ren, Yujun et al. Overexpressing BrWRKY22 Delays Flowering and Leaf Senescence via Inhibition of GA Biosynthesis in Brassica rapa . | PLANTS-BASEL , 2025 , 14 (11) . |
| Export to | NoteExpress RIS BibTex |
Version ：
Abstract ：
Drought is a critical risk factor that impacts rice growth and yields. Previous studies have focused on the regulatory roles of individual transcription factors in response to drought stress. However, there is limited understanding of multi-factor stresses gene regulatory networks and their mechanisms of action. In this study, we utilised data from the JASPAR database to compile a comprehensive dataset of transcription factors and their binding sites in rice, Arabidopsis, and barley genomes. We employed the PyTorch framework for machine learning to develop a nine-layer convolutional deep neural network TFBind. Subsequently, we obtained rice RNA-seq and ATAC-seq data related to abiotic stress from the public database. Utilising integrative analysis of WGCNA and ATAC-seq, we effectively identified transcription factors associated with open chromatin regions in response to drought. Interestingly, only 81% of the transcription factors directly bound to the opened genes by testing with TFBind model. By this approach we identified 15 drought-responsive transcription factors corresponding to open chromatin regions of targets, which enriched in the terms related to protein transport, protein allocation, nitrogen compound transport. This approach provides a valuable tool for predicting TF-TAG-opened modules during biological processes.
Keyword ：
ATAC-seq ATAC-seq drought responsive TF-TAG modules drought responsive TF-TAG modules neural networks neural networks rice rice WGCNA WGCNA
Cite：
Copy from the list or Export to your reference management。
| GB/T 7714 | Liu, Jingpeng , Shi, Ximiao , Zhang, Zhitai et al. Deep Neural Network-Mining of Rice Drought-Responsive TF-TAG Modules by a Combinatorial Analysis of ATAC-Seq and RNA-Seq [J]. | PLANT CELL AND ENVIRONMENT , 2025 , 48 (7) : 5217-5235 . |
| MLA | Liu, Jingpeng et al. "Deep Neural Network-Mining of Rice Drought-Responsive TF-TAG Modules by a Combinatorial Analysis of ATAC-Seq and RNA-Seq" . | PLANT CELL AND ENVIRONMENT 48 . 7 (2025) : 5217-5235 . |
| APA | Liu, Jingpeng , Shi, Ximiao , Zhang, Zhitai , Cen, Xuexiang , Lin, Lixian , Wang, Xiaowei et al. Deep Neural Network-Mining of Rice Drought-Responsive TF-TAG Modules by a Combinatorial Analysis of ATAC-Seq and RNA-Seq . | PLANT CELL AND ENVIRONMENT , 2025 , 48 (7) , 5217-5235 . |
| Export to | NoteExpress RIS BibTex |
Version ：
Abstract ：
Seed germination is a complex process influenced by various physical and biochemical factors, and dormancy release upon imbibition is essential for germination. However, the specific regulators controlling dormancy release in rice remain largely unknown. Here, we identified OsS40-14, a member of the senescence-associated S40 gene family, as a negative regulator of seed germination in rice. Using CRISPR/Cas9 gene editing and transgenic overexpression (OE) lines, we found that the oss40-14 mutant lines exhibit preharvest sprouting (PHS) and accelerated germination, while the OE-OsS40-14 lines show delayed germination. Notably, dormancy-breaking treatments largely eliminated these phenotypic differences. Analysis of endogenous abscisic acid (ABA) levels and the response to exogenous ABA revealed that OsS40-14 regulates germination by modulating ABA signaling. Transcriptomic and Cleavage under targets and tagmentation (CUT & Tag)-seq analyses demonstrated that OsS40-14 directly represses the transcription of two ABA catabolic genes, OsABA8ox1 and OsABA8ox3, thereby preventing the reduction of ABA levels during seed dormancy release. These findings establish OsS40-14 as a key regulator of seed germination through its control of ABA homeostasis, providing potential targets for breeding PHS-resistant varieties. OsS40-14 negatively regulates rice seed germination by directly repressing abscisic acid (ABA) catabolic genes to maintain ABA levels, thereby preventing dormancy release and enhancing preharvest sprouting resistance.
Cite：
Copy from the list or Export to your reference management。
| GB/T 7714 | Zheng, Xiangzi , Wang, Weiqi , Fan, Chunlan et al. OsS40-14 regulates rice seed germination by modulating ABA responsiveness and inhibiting dormancy release [J]. | PLANT PHYSIOLOGY , 2025 , 199 (2) . |
| MLA | Zheng, Xiangzi et al. "OsS40-14 regulates rice seed germination by modulating ABA responsiveness and inhibiting dormancy release" . | PLANT PHYSIOLOGY 199 . 2 (2025) . |
| APA | Zheng, Xiangzi , Wang, Weiqi , Fan, Chunlan , Tan, Min , Dai, Huiling , Liao, Dongmei et al. OsS40-14 regulates rice seed germination by modulating ABA responsiveness and inhibiting dormancy release . | PLANT PHYSIOLOGY , 2025 , 199 (2) . |
| Export to | NoteExpress RIS BibTex |
Version ：
Abstract ：
Plasma membrane (PM) H+-ATPases, well known as proton pumps, are P-type IIIA ATPases that regulate numerous aspects of plant growth, development and response to environmental stimuli by generating trans-membrane pH gradients (Delta pH) and electrochemical potentials (Delta Psi) across the plasma membrane. Nonetheless, the recent determinants of active pH buffering and proton pump-mediated ion homeostasis have not yet been considered and are still a matter of discussion. In this review, we first classify the distinct P-type ATPases and review the mechanism of H+ transport across plasma membranes by PM H+-ATPases, and then focus on how PM H+-ATPases together with cyclic nucleotide-gated channels, cation/proton antiporters and other metabolism-related processes control cytoplasmic pH and ion homeostasis across cell membranes in plants under both normal and stress conditions.
Keyword ：
Cation/proton antiporters Cation/proton antiporters Cyclic nucleotide-gated channels Cyclic nucleotide-gated channels Metabolism Metabolism Plasma membrane H plus -ATPase Plasma membrane H plus -ATPase P-type ATPase P-type ATPase
Cite：
Copy from the list or Export to your reference management。
| GB/T 7714 | Li, Qian , Jiang, Xin , Liu, Yi et al. Recent advances in understanding the P-type IIIA ATPase-mediated cellular pH and ion homeostasis in plants adapt to environmental changes [J]. | PLANT PHYSIOLOGY AND BIOCHEMISTRY , 2025 , 229 . |
| MLA | Li, Qian et al. "Recent advances in understanding the P-type IIIA ATPase-mediated cellular pH and ion homeostasis in plants adapt to environmental changes" . | PLANT PHYSIOLOGY AND BIOCHEMISTRY 229 (2025) . |
| APA | Li, Qian , Jiang, Xin , Liu, Yi , Han, Zhiwei , Zhu, Pan , Miao, Ying et al. Recent advances in understanding the P-type IIIA ATPase-mediated cellular pH and ion homeostasis in plants adapt to environmental changes . | PLANT PHYSIOLOGY AND BIOCHEMISTRY , 2025 , 229 . |
| Export to | NoteExpress RIS BibTex |
Version ：
Abstract ：
Multiple organellar RNA editing factor (MORF) complex was shown to be highly associated with C-to-U RNA editing of vascular plant editosome. However, mechanisms by which MORF9-dependent plastid RNA editing controls plant development and responses to environmental alteration remain obscure. In this study, we found that loss of MORF9 function impaired PSII efficiency, NDH activity, and carbohydrate production, rapidly promoted nuclear gene expression including sucrose transporter and sugar/energy responsive genes, and attenuated root growth under sugar starvation conditions. Sugar repletion increased MORF9 and MORF2 expression in wild-type seedlings and reduced RNA editing of matK-706, accD-794, ndhD-383 and ndhF-290 in the morf9 mutant. RNA editing efficiency of ndhD-383 and ndhF-290 sites was diminished in the gin2/morf9 double mutants, and that of matK-706, accD-794, ndhD-383 and ndhF-290 sites were significantly diminished in the snrk1/morf9 double mutants. In contrast, overexpressing HXK1 or SnRK1 promoted RNA editing rate of matK-706, accD-794, ndhD-383 and ndhF-290 in leaves of morf9 mutants, suggesting that HXK1 partially impacts MORF9 mediated ndhD-383 and ndhF-290 editing, while SnRK1 may only affect MORF9-mediated ndhF-290 site editing. Collectively, these findings suggest that sugar and/or its intermediary metabolites impair MORF9-dependent plastid RNA editing resulting in derangements of plant root development. Sugar supplementation alters MORF9 levels and MORF9-dependent plastid RNA editing, which in turn affects nuclear gene expression and root growth in Arabidopsis, suggesting that plastid RNA editing regulates auxin-related root growth and sugar starvation response via a retrograde signalling pathway.
Keyword ：
MORF MORF root development root development sugar response/regulation sugar response/regulation
Cite：
Copy from the list or Export to your reference management。
| GB/T 7714 | Xie, Yakun , Yu, Jinfa , Tian, Faan et al. MORF9-dependent specific plastid RNA editing inhibits root growth under sugar starvation in Arabidopsis [J]. | PLANT CELL AND ENVIRONMENT , 2024 , 47 (6) : 1921-1940 . |
| MLA | Xie, Yakun et al. "MORF9-dependent specific plastid RNA editing inhibits root growth under sugar starvation in Arabidopsis" . | PLANT CELL AND ENVIRONMENT 47 . 6 (2024) : 1921-1940 . |
| APA | Xie, Yakun , Yu, Jinfa , Tian, Faan , Li, Xue , Chen, Xinyan , Li, Yanyun et al. MORF9-dependent specific plastid RNA editing inhibits root growth under sugar starvation in Arabidopsis . | PLANT CELL AND ENVIRONMENT , 2024 , 47 (6) , 1921-1940 . |
| Export to | NoteExpress RIS BibTex |
Version ：
Abstract ：
Plant senescence is an integrated programme of plant development that aims to remobilize nutrients and energy from senescing tissues to developing organs under developmental and stress-induced conditions. Upstream in the regulatory network, a small family of single-stranded DNA/RNA-binding proteins known as WHIRLYs occupy a central node, acting at multiple regulatory levels and via trans-localization between the nucleus and organelles. In this review, we summarize the current progress on the role of WHIRLY members in plant development and stress-induced senescence. WHIRLY proteins can be traced back in evolution to green algae. WHIRLY proteins trade off the balance of plant developmental senescence and stress-induced senescence through maintaining organelle genome stability via R-loop homeostasis, repressing the transcription at a configuration condition, and recruiting RNA to impact organelle RNA editing and splicing, as evidenced in several species. WHIRLY proteins also act as retrograde signal transducers between organelles and the nucleus through protein modification and stromule or vesicle trafficking. In addition, WHIRLY proteins interact with hormones, reactive oxygen species and environmental signals to orchestrate cell fate in an age-dependent manner. Finally, prospects for further research and promotion to improve crop production under environmental constraints are highlighted.
Keyword ：
Evolutionary Evolutionary organelle-nucleus retrograde signals organelle-nucleus retrograde signals plant developmental senescence plant developmental senescence regulatory mechanism regulatory mechanism stress-induced senescence stress-induced senescence WHIRLY family WHIRLY family
Cite：
Copy from the list or Export to your reference management。
| GB/T 7714 | Lin, Wenfang , Huang, Dongmei , Li, Mengsi et al. WHIRLY proteins, multi-layer regulators linking the nucleus and organelles in developmental and stress-induced senescence of plants [J]. | ANNALS OF BOTANY , 2024 , 134 (4) : 521-536 . |
| MLA | Lin, Wenfang et al. "WHIRLY proteins, multi-layer regulators linking the nucleus and organelles in developmental and stress-induced senescence of plants" . | ANNALS OF BOTANY 134 . 4 (2024) : 521-536 . |
| APA | Lin, Wenfang , Huang, Dongmei , Li, Mengsi , Ren, Yujun , Zheng, Xiangzi , Wu, Binghua et al. WHIRLY proteins, multi-layer regulators linking the nucleus and organelles in developmental and stress-induced senescence of plants . | ANNALS OF BOTANY , 2024 , 134 (4) , 521-536 . |
| Export to | NoteExpress RIS BibTex |
Version ：
Export
| Results： |
Selected to |
| Format： |
