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学者姓名：王秀娜
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Abstract ：
The bZIP-type transcription factors (TFs) are widely distributed in eukaryotes and display various roles. The aim of this study was to characterize a bZIP-type TF in an opportunistic pathogen Aspergillus flavus (AfZIF1), which produced carciongenic and mutagenic aflatoxins. Deletion of AfZIF1 significantly reduced condidiogenesis, while increased the number of sclerotia but reduced its size. Compared to wild type (WT) and complemented strain (∆AfZIF1C), deleted strain (∆AfZIF1) displayed resistance to high-osmolarity reagent KCl and cell membrane stress reagent CuSO4, but increased sensitivity to Congo red. Further analysis indicated that the chitin content of ΔAfZIF1 strain was higher than that of WT and ΔAfZIF1C. Besides, ΔAfZIF1 showed impairment in hydrophobicity. The significant increase of aflatoxin B1 produced by ∆AfZIF1 was both on medium and on peanut. Besides, ∆AfZIF1 mutant was defective in virulence on peanut. Transcriptome analysis and qRT-PCR further provided evidence that AfZIF1 globally regulated genes involved in production of conidia and sclerotia, cell wall integrity, and aflatoxins biosynthesis. Our findings revealed that AfZIF1 as a global regulator has broad regulatory functions, and also provide novel insights into the regulatory mechanisms of the transcription factor AfZIF1 in afltoxins biosynthesis. © 2024, The Authors. All rights reserved.
Keyword ：
Aflatoxins Aflatoxins Aspergillus Aspergillus Biosynthesis Biosynthesis Cell membranes Cell membranes Transcription Transcription Transcription factors Transcription factors
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| GB/T 7714 | Wang, Xiuna , Yao, Bin , Zha, Wenjie et al. Global Regulator Afzif1 Modulates Development, Cell Wall Integrity, and Aflatoxin B1 Production in Aspergillus Flavus [J]. | SSRN , 2024 . |
| MLA | Wang, Xiuna et al. "Global Regulator Afzif1 Modulates Development, Cell Wall Integrity, and Aflatoxin B1 Production in Aspergillus Flavus" . | SSRN (2024) . |
| APA | Wang, Xiuna , Yao, Bin , Zha, Wenjie , Chen, Yajuan , Guo, Huihua , Sui, Hanyu et al. Global Regulator Afzif1 Modulates Development, Cell Wall Integrity, and Aflatoxin B1 Production in Aspergillus Flavus . | SSRN , 2024 . |
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Aspergillus flavus produces carcinogenic and mutagenic aflatoxins, which cause economic losses and risk of food safety by contaminating grains, food and feed. In this study, we characterized two bZIP transcription factors, AflatfA and AflatfB, and their genetic interaction. Compared to the wild type (WT), AflatfA deletion and AflatfA and AflatfB double deletion both caused retarded vegetative growth of mycelia. Relative to WT, the AflatfA deletion strain (Delta AflatfA) and AflatfA and AflatfB double deletion strain (Delta AflatfA Delta AflatfB) produced more sclerotia, whereas the AflatfB deletion strain (Delta AflatfB) produced less sclerotia. After 4 degrees C preservation and incubation at 50 degrees C, conidia viability dramatically decreased in the Delta AflatfA and Delta AflatfA Delta AflatfB but Delta AflatfB mutants, whereas conidia viability of the Delta AflatfA Delta AflatfB strain was higher after storage at 4 degrees C than in AflatfA mutant. Conidia of Delta AflatfA, Delta AflatfB and Delta AflatfA Delta AflatfB strains significantly increased in sensitivity to H2O2 in comparison with WT. Compared to WT, the mycelium of Delta AflatfA and Delta AflatfB strains were more sensitive to H2O2; conversely, the Delta AflatfA Delta AflatfB strain showed less sensitivity to H2O2. Delta AflatfA and Delta AflatfA Delta AflatfB strains displayed less sensitivity to the osmotic reagents NaCl, KCl and Sorbitol, in comparison with WT and Delta AflatfB strains. When on YES medium and hosts corn and peanut, Delta AflatfA and Delta AflatfA Delta AflatfB strains produced less aflatoxin B1 (AFB1) than Delta AflatfB, and the AFB1 yield of Delta AflatfB was higher than that of WT. When WT and mutants were inoculated on corn and peanut, the Delta AflatfA and Delta AflatfA Delta AflatfB but not Delta AflatfB mutants produced less conidia than did WT. Taken together, this study reveals that AflatfA controls more cellular processes, and the function of AflatfA is stronger than that of AflatfB when of the same process is regulated, except the response to H2O2, which might result from the effect of AflatfA on the transcriptional level of AflatfB.
Keyword ：
AflatfA AflatfA AflatfB AflatfB aflatoxin B1 aflatoxin B1 Aspergillus flavus Aspergillus flavus bZIP transcription factor bZIP transcription factor development development stress response stress response
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| GB/T 7714 | Wang, Xiuna , Zha, Wenjie , Yao, Bin et al. Genetic Interaction of Global Regulators AflatfA and AflatfB Mediating Development, Stress Response and Aflatoxins B1 Production in Aspergillus flavus [J]. | TOXINS , 2022 , 14 (12) . |
| MLA | Wang, Xiuna et al. "Genetic Interaction of Global Regulators AflatfA and AflatfB Mediating Development, Stress Response and Aflatoxins B1 Production in Aspergillus flavus" . | TOXINS 14 . 12 (2022) . |
| APA | Wang, Xiuna , Zha, Wenjie , Yao, Bin , Yang, Lan , Wang, Shihua . Genetic Interaction of Global Regulators AflatfA and AflatfB Mediating Development, Stress Response and Aflatoxins B1 Production in Aspergillus flavus . | TOXINS , 2022 , 14 (12) . |
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免疫学是一门既古老又年轻的综合学科。随着现代免疫学的建立,免疫学的发展日新月异,并从专业领域逐步走向大众。为落实"以德树人"的教育目标,探索"免疫学导论"课程思政的开展,以激发学生学习兴趣,使之更好地掌握和理解知识点,同时提高学生的人文素养,厚植爱国情怀,以绪论中疫苗的研究历史为教学案例,将知识点、思政元素和新冠疫情背景相融合,探索适合农业院校开展免疫学课程思政的新路径。
Keyword ：
免疫学导论 免疫学导论 案例分析 案例分析 疫苗 疫苗 课程思政 课程思政
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| GB/T 7714 | 林玲 , 王秀娜 , 袁军 et al. “免疫学导论”课程思政的教学实践探索——以“疫苗的前世今生”为教学案例 [J]. | 黑龙江生态工程职业学院学报 , 2021 , 34 (04) : 148-151 . |
| MLA | 林玲 et al. "“免疫学导论”课程思政的教学实践探索——以“疫苗的前世今生”为教学案例" . | 黑龙江生态工程职业学院学报 34 . 04 (2021) : 148-151 . |
| APA | 林玲 , 王秀娜 , 袁军 , 陈煜 . “免疫学导论”课程思政的教学实践探索——以“疫苗的前世今生”为教学案例 . | 黑龙江生态工程职业学院学报 , 2021 , 34 (04) , 148-151 . |
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以杂交稻T78优2155为材料,在人工移栽模式下,对早稻和晚稻接种纹枯病菌,并研究氮、磷、钾肥用量对水稻纹枯病病斑高度和产量的影响.结果表明:水稻未施肥时纹枯病发生最轻;相同肥料处理在不同年份对纹枯病的影响不同;肥料用量显著影响了2019和2020年晚稻的纹枯病发生程度.2020年晚稻在未接种纹枯病菌时,未施肥的产量显著低于施用肥料的处理;氮、磷、钾肥用量未对接菌的水稻产量造成显著影响,且纹枯病严重程度与水稻产量之间无相关性;2019年未接菌早稻未施肥的千粒重显著低于施用肥料的处理.由此可见,合理使用氮、磷、钾肥可以防控水稻纹枯病,且在一定范围内增减其用量不会影响水稻产量.
Keyword ：
产量 产量 氮 氮 水稻纹枯病 水稻纹枯病 磷 磷 钾 钾
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| GB/T 7714 | 王秀娜 , 董明科 , 汪世华 . 氮、磷、钾肥用量对水稻纹枯病病斑高度及产量的影响 [J]. | 福建农林大学学报(自然科学版) , 2021 , 50 (03) : 317-322 . |
| MLA | 王秀娜 et al. "氮、磷、钾肥用量对水稻纹枯病病斑高度及产量的影响" . | 福建农林大学学报(自然科学版) 50 . 03 (2021) : 317-322 . |
| APA | 王秀娜 , 董明科 , 汪世华 . 氮、磷、钾肥用量对水稻纹枯病病斑高度及产量的影响 . | 福建农林大学学报(自然科学版) , 2021 , 50 (03) , 317-322 . |
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本发明提供一株防治水稻纹枯病的生防菌株苏云金芽孢杆菌FJ2B‑25，属于植物保护技术领域。生防菌株苏云金芽孢杆菌FJ2B‑25已于2021年3月15日保藏在中国微生物菌种保藏管理委员会普通微生物中心，保藏号为CGMCC No.22014。生防菌株苏云金芽孢杆菌FJ2B‑25及生防菌株苏云金芽孢杆菌FJ2B‑25的无菌体发酵液对水稻纹枯病病原菌立枯丝核菌(Rhizoctonia solani)具有显著的抑制作用。该菌株在开发水稻纹枯病绿色防治的生防菌剂并分离具抑菌活性的化合物方面具有广阔应用前景。
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| GB/T 7714 | 王秀娜 , 董明科 , 汪世华 et al. 一株防治水稻纹枯病的生防菌株苏云金芽孢杆菌FJ2B-25 : CN202110718220.8[P]. | 2021-06-28 . |
| MLA | 王秀娜 et al. "一株防治水稻纹枯病的生防菌株苏云金芽孢杆菌FJ2B-25" : CN202110718220.8. | 2021-06-28 . |
| APA | 王秀娜 , 董明科 , 汪世华 , 吴惠娟 , 李加翼 . 一株防治水稻纹枯病的生防菌株苏云金芽孢杆菌FJ2B-25 : CN202110718220.8. | 2021-06-28 . |
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本发明提供一株蜡样芽孢杆菌(Bacillus cereus)FJ2B‑137在水稻纹枯病生物防治中的应用，属于植物病害生物防治领域。2021年3月15日已将蜡样芽孢杆菌(Bacillus cereus)FJ2B‑137保藏于中国微生物菌种保藏管理委员会普通微生物中心，保藏号为CGMCC No.22015。蜡样芽孢杆菌(Bacillus cereus)FJ2B‑137及其无菌体发酵液在抑制水稻纹枯病病原菌立枯丝核菌(Rhizoctonia solani)菌丝生长及菌核萌发方面具有显著作用。该菌株为开发水稻纹枯病病原菌立枯丝核菌(Rhizoctonia solani)防治的复合菌剂提供菌株资源。
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| GB/T 7714 | 王秀娜 , 董明科 , 汪世华 et al. 一株蜡样芽孢杆菌FJ2B-137在水稻纹枯病生物防治中的应用 : CN202110813755.3[P]. | 2021-07-19 . |
| MLA | 王秀娜 et al. "一株蜡样芽孢杆菌FJ2B-137在水稻纹枯病生物防治中的应用" : CN202110813755.3. | 2021-07-19 . |
| APA | 王秀娜 , 董明科 , 汪世华 , 吴惠娟 , 李加翼 . 一株蜡样芽孢杆菌FJ2B-137在水稻纹枯病生物防治中的应用 : CN202110813755.3. | 2021-07-19 . |
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sigma(54) factor (RpoN), a type of transcriptional regulatory factor, is widely found in pathogenic bacteria. It binds to core RNA polymerase (RNAP) and regulates the transcription of many functional genes in an enhancer-binding protein (EBP)-dependent manner. sigma(54) has two conserved functional domains: the activator-interacting domain located at the N-terminal and the DNA-binding domain located at the C-terminal. RpoN directly binds to the highly conserved sequence, GGN(10)GC, at the -24/-12 position relative to the transcription start site of target genes. In general, bacteria contain one or two RpoNs but multiple EBPs. A single RpoN can bind to different EBPs in order to regulate various biological functions. Thus, the overlapping and unique regulatory pathways of two RpoNs and multiple EBP-dependent regulatory pathways form a complex regulatory network in bacteria. However, the regulatory role of RpoN and EBPs is still poorly understood in phytopathogenic bacteria, which cause economically important crop diseases and pose a serious threat to world food security. In this review, we summarize the current knowledge on the regulatory function of RpoN, including swimming motility, flagella synthesis, bacterial growth, type IV pilus (T4Ps), twitching motility, type III secretion system (T3SS), and virulence-associated phenotypes in phytopathogenic bacteria. These findings and knowledge prove the key regulatory role of RpoN in bacterial growth and pathogenesis, as well as lay the groundwork for further elucidation of the complex regulatory network of RpoN in bacteria.
Keyword ：
enhancer-binding proteins enhancer-binding proteins motility motility sigma(54) factor sigma(54) factor T3SS T3SS virulence virulence
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| GB/T 7714 | Yu, Chao , Yang, Fenghuan , Xue, Dingrong et al. The Regulatory Functions of σ54 Factor in Phytopathogenic Bacteria [J]. | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES , 2021 , 22 (23) . |
| MLA | Yu, Chao et al. "The Regulatory Functions of σ54 Factor in Phytopathogenic Bacteria" . | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 22 . 23 (2021) . |
| APA | Yu, Chao , Yang, Fenghuan , Xue, Dingrong , Wang, Xiuna , Chen, Huamin . The Regulatory Functions of σ54 Factor in Phytopathogenic Bacteria . | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES , 2021 , 22 (23) . |
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【目的】蜜蜂球囊菌(Ascosphaera apis,简称球囊菌)是一种专性侵染蜜蜂幼虫的真菌病原,导致的白垩病是严重影响养蜂生产的顽疾,每年给养蜂业造成较大损失。本研究旨在基于已获得的第三代长读段测序数据对球囊菌菌丝(Aam)和孢子(Aas)中基因的可变剪切(alternative splicing,AS)和可变多聚腺苷酸化(alternative polyadenylation,APA)进行深入分析。【方法】利用Astalavista软件鉴定Aam和Aas中基因的AS事件类型。利用IGV浏览器对部分剪切异构体(isoform)的结构进行可视化。通过TAPIS pipeline对Aam和Aas中基因的APA位点进行鉴定。通过MEME软件对Aam和Aas中全长转录本的APA位点上游50bp的序列特征进行分析并对motif进行鉴定。【结果】在Aam中共鉴定到286次AS事件,包括162次RI(Retained intron),87次A3(Alternative 3'splice-site)、32次A5(Alternative 5'splice-site)和5次SE (Skipping exon);在Aas中共鉴定到559次AS事件,包括305次RI、155次A3、85次A5、13次SE和1次MEE (Mutually exclusive exon)。进一步分析发现,现有参考基因组上的多数注释基因结构并不完整;部分注释基因在菌丝和孢子中转录形成的isoform在数量和结构方面均存在差异;部分isoform在参考基因组中没有对应的注释基因。Aam中共鉴定到2748个基因含有1个及以上的APA位点,其中含有1个APA位点的基因数量最多(726,26.42%);Aas中共鉴定到2768个基因含有1个及以上的APA位点,其中含有5个以上APA位点的基因数量最多(1180,42.63%)。部分基因在菌丝和孢子中含有不同的APA位点数。序列特征分析结果显示,球囊菌全长转录本的3'UTR的上下游表现出明显的碱基倾向性,U和A分别富集在3'UTR的上游和下游。此外,在球囊菌全长转录本的APA位点上游鉴定到4个motif,分别是UCUCCU、UCUUCU、CCCACC和CCCCCU。【结论】本研究通过对球囊菌菌丝和孢子中基因的AS和APA进行深入分析,揭示了球囊菌转录组的复杂性,为完善现有的基因组和转录组注释提供了宝贵信息,也为探究AS和APA在球囊菌的基因表达调控中的作用提供了关键基础。
Keyword ：
可变剪切 可变剪切 可变腺苷酸化 可变腺苷酸化 牛津纳米孔 牛津纳米孔 蜜蜂球囊菌 蜜蜂球囊菌 长读段测序 长读段测序
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| GB/T 7714 | 杜宇 , 王杰 , 蒋海宾 et al. 基于第三代长读段测序数据解析蜜蜂球囊菌基因的可变剪切与可变腺苷酸化 [J]. | 微生物学报 , 2021 , 61 (03) : 667-682 . |
| MLA | 杜宇 et al. "基于第三代长读段测序数据解析蜜蜂球囊菌基因的可变剪切与可变腺苷酸化" . | 微生物学报 61 . 03 (2021) : 667-682 . |
| APA | 杜宇 , 王杰 , 蒋海宾 , 王秀娜 , 范元婵 , 范小雪 et al. 基于第三代长读段测序数据解析蜜蜂球囊菌基因的可变剪切与可变腺苷酸化 . | 微生物学报 , 2021 , 61 (03) , 667-682 . |
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【目的】本研究旨在探究蜜蜂球囊菌Ascosphaera apis(简称"球囊菌")菌丝(Aam)和孢子(Aas)中的全长转录本与菌丝生长、孢子萌发以及有性生殖的相关性。【方法】将前期获得的Aam和Aas的Nanopore长读段测序数据中的有效读段与球囊菌参考基因组注释的已知转录本进行序列比对,获取全长转录本与已知转录本的对应信息。利用生物信息学方法对Aam和Aas的全长转录本进行差异表达、功能注释以及结构特征分析。【结果】Aam和Aas共有的全长转录本数量为19 966条,特有的全长转录本数量分别为1 273和2 856条。Aas vs Aam比较组含有3 230条差异表达转录本(differentially expressed transcripts, DETs),包含3 072条上调和158条下调。GO功能注释结果显示,这些DETs涉及代谢进程、细胞、催化活性等GO条目。KEGG注释结果显示,这些DETs注释到内吞作用、MAPK信号通路、糖酵解/糖异生、碳代谢以及氨基酸的生物合成等相关通路。进一步分析发现,部分全长转录本的剪接异构体在Aam和Aas中具有不同的表达量和结构。【结论】本研究发现球囊菌在菌丝和孢子两个不同阶段伴随着转录本表达量和结构的变化,研究结果为深入探究不同剪接异构体在球囊菌的菌丝生长、孢子萌发和有性生殖中的分子功能提供了理论依据和数据基础。
Keyword ：
三代测序 三代测序 全长转录本 全长转录本 孢子 孢子 差异表达转录本 差异表达转录本 菌丝 菌丝 蜜蜂球囊菌 蜜蜂球囊菌
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| GB/T 7714 | 杜宇 , 蒋海宾 , 王杰 et al. 蜜蜂球囊菌菌丝和孢子中全长转录本的差异表达分析 [J]. | 昆虫学报 , 2021 , 64 (03) : 363-373 . |
| MLA | 杜宇 et al. "蜜蜂球囊菌菌丝和孢子中全长转录本的差异表达分析" . | 昆虫学报 64 . 03 (2021) : 363-373 . |
| APA | 杜宇 , 蒋海宾 , 王杰 , 范小雪 , 王秀娜 , 冯睿蓉 et al. 蜜蜂球囊菌菌丝和孢子中全长转录本的差异表达分析 . | 昆虫学报 , 2021 , 64 (03) , 363-373 . |
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【目的】利用已获得的纳米孔长读段测序数据完善现有的蜜蜂球囊菌Ascosphaera apis参考基因组注释信息,并对未注释的新基因和新转录本进行鉴定和功能注释。【方法】基于已获得的纳米孔长读段测序数据,采用gffcompare软件将蜜蜂球囊菌全长转录本与参考基因组注释的转录本进行比较,进而对参考基因组注释基因的非翻译区(untranslated region, UTR)进行延长。利用TransDecoder软件对蜜蜂球囊菌基因的开放阅读框(open reading frame, ORF)及相应的氨基酸序列进行预测。通过MISA软件发掘长度在500 bp以上的全长转录本的SSR位点。通过Blast工具将鉴定到的新基因和新转录本比对Nr, KOG, eggNOG, Swiss-Prot, Pfam, GO和KEGG数据库进行功能注释。【结果】共对蜜蜂球囊菌的9 481个基因进行了UTR延长,其中5′UTR和3′UTR延长的基因分别有4 744和4 737个。共预测出10 492个完整ORF,其中编码长度分布在0～100和100～200个氨基酸的ORF最多,分别占ORF总数的38.96%和36.90%。共鉴定到5 286个SSR,其中单核苷酸重复、二核苷酸重复、三核苷酸重复、四核苷酸重复、五核苷酸重复和六核苷酸重复的SSR分别为1 870, 826, 2 398, 138, 43和11个。共鉴定到1 558个新基因,其中有1 556, 731, 330, 592, 1 177, 709和589个新基因可分别被注释到Nr, Swiss-Prot, Pfam, KOG, eggNOG, GO和KEGG数据库。此外,还鉴定到14 403条新转录本,其中有14 376, 8 524, 7 276, 7 405, 12 035, 7 891和6 855条新转录本可分别被注释到上述7个数据库。【结论】本研究利用已获得的纳米孔长读段测序数据对蜜蜂球囊菌的完整ORF进行了预测,对参考基因组的已注释基因进行了UTR延长,对未注释的SSR位点进行了发掘,此外还鉴定到大量未注释的新基因和新转录本,并对它们进行了功能注释。研究结果较好地完善了现有的蜜蜂球囊菌的基因组注释,为其组学和分子生物学研究的深入开展提供了基础。
Keyword ：
全长转录组 全长转录组 基因组 基因组 白垩病 白垩病 蜜蜂 蜜蜂 蜜蜂球囊菌 蜜蜂球囊菌 长读段测序技术 长读段测序技术
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| GB/T 7714 | 杜宇 , 付中民 , 祝智威 et al. 基于蜜蜂球囊菌纳米孔测序数据的基因非翻译区延长、SSR位点发掘及未注释基因和转录本鉴定 [J]. | 昆虫学报 , 2020 , 63 (11) : 1345-1357 . |
| MLA | 杜宇 et al. "基于蜜蜂球囊菌纳米孔测序数据的基因非翻译区延长、SSR位点发掘及未注释基因和转录本鉴定" . | 昆虫学报 63 . 11 (2020) : 1345-1357 . |
| APA | 杜宇 , 付中民 , 祝智威 , 王杰 , 冯睿蓉 , 王秀娜 et al. 基于蜜蜂球囊菌纳米孔测序数据的基因非翻译区延长、SSR位点发掘及未注释基因和转录本鉴定 . | 昆虫学报 , 2020 , 63 (11) , 1345-1357 . |
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