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学者姓名：苗锐
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Abstract ：
OsYchF1 belongs to the YchF subfamily in the Obg family of G proteins, and comprises a core G domain, an alpha-helical domain, and a TGS domain from the N-terminus to the C-terminus. The C2-domain Oryza sativa GTPase-activating protein (OsGAP1) binds to and accelerates the enzyme activity of OsYchF1. The OsYchF1-OsGAP1 module plays a pivotal role in the response of rice to environmental stress. Nonetheless, how OsYchF1 anchors OsGAP1 has remained elusive. Our results demonstrate that OsYchF1 interacts with OsGAP1 through its N-terminal G domain and C-terminal TGS domain, while the inserted helical domain imposes a steric hindrance, as shown by yeast two-hybrid and isothermal titration calorimetry assays. Also, we implemented the AlphaFold2 artificial intelligence system to predict the structure of the OsYchF1-OsGAP1 complex. However, AlphaFold2 could not capture these dynamic conformational changes, likely due to the weak (K-D=102.6 +/- 11.7 mu M) and transient nature of the OsYchF1-OsGAP1 interaction, which is primarily driven by hydrophobic interactions. Unexpectedly, the protein-protein docking by a fully automated algorithm ClusPro accurately recapitulated both the experimental evidence for G/TGS domain-mediated binding and the known cluster regions on OsGAP1, whereas AlphaFold2 failed to align with these findings.
Keyword ：
AlphaFold2 AlphaFold2 C2-domain OsGAP1 C2-domain OsGAP1 ClusPro 2.0 ClusPro 2.0 G domain G domain Helical domain Helical domain Isothermal titration calorimetry Isothermal titration calorimetry OsYchF1 OsYchF1 TGS domain TGS domain Unconventional g protein Unconventional g protein Yeast two-hybrid Yeast two-hybrid
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| GB/T 7714 | Huang, Suchang , Wu, Ziting , Wang, Kuang et al. From biophysical interaction to structural modeling: bi-terminal G and TGS domains drive rice OsYchF1-OsGAP1 complex formation [J]. | BOTANICAL STUDIES , 2025 , 66 (1) . |
| MLA | Huang, Suchang et al. "From biophysical interaction to structural modeling: bi-terminal G and TGS domains drive rice OsYchF1-OsGAP1 complex formation" . | BOTANICAL STUDIES 66 . 1 (2025) . |
| APA | Huang, Suchang , Wu, Ziting , Wang, Kuang , Liao, Yunchuan , Zeng, Jiaqiang , Miao, Rui . From biophysical interaction to structural modeling: bi-terminal G and TGS domains drive rice OsYchF1-OsGAP1 complex formation . | BOTANICAL STUDIES , 2025 , 66 (1) . |
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Plasma membrane (PM) H+-ATPases, well known as proton pumps, are P-type IIIA ATPases that regulate numerous aspects of plant growth, development and response to environmental stimuli by generating trans-membrane pH gradients (Delta pH) and electrochemical potentials (Delta Psi) across the plasma membrane. Nonetheless, the recent determinants of active pH buffering and proton pump-mediated ion homeostasis have not yet been considered and are still a matter of discussion. In this review, we first classify the distinct P-type ATPases and review the mechanism of H+ transport across plasma membranes by PM H+-ATPases, and then focus on how PM H+-ATPases together with cyclic nucleotide-gated channels, cation/proton antiporters and other metabolism-related processes control cytoplasmic pH and ion homeostasis across cell membranes in plants under both normal and stress conditions.
Keyword ：
Cation/proton antiporters Cation/proton antiporters Cyclic nucleotide-gated channels Cyclic nucleotide-gated channels Metabolism Metabolism Plasma membrane H plus -ATPase Plasma membrane H plus -ATPase P-type ATPase P-type ATPase
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| GB/T 7714 | Li, Qian , Jiang, Xin , Liu, Yi et al. Recent advances in understanding the P-type IIIA ATPase-mediated cellular pH and ion homeostasis in plants adapt to environmental changes [J]. | PLANT PHYSIOLOGY AND BIOCHEMISTRY , 2025 , 229 . |
| MLA | Li, Qian et al. "Recent advances in understanding the P-type IIIA ATPase-mediated cellular pH and ion homeostasis in plants adapt to environmental changes" . | PLANT PHYSIOLOGY AND BIOCHEMISTRY 229 (2025) . |
| APA | Li, Qian , Jiang, Xin , Liu, Yi , Han, Zhiwei , Zhu, Pan , Miao, Ying et al. Recent advances in understanding the P-type IIIA ATPase-mediated cellular pH and ion homeostasis in plants adapt to environmental changes . | PLANT PHYSIOLOGY AND BIOCHEMISTRY , 2025 , 229 . |
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The polyhydroxylated steroid phytohormone brassinosteroid (BR) controls many aspects of plant growth, development and responses to environmental changes. Plasma membrane (PM) H+-ATPase, the well-known PM proton pump, is a central regulator in plant physiology, which mediates not only plant growth and development, but also adaptation to stresses. Recent studies highlight that PM H+-ATPase is at least partly regulated via the BR signaling. Firstly, the BR cell surface receptor BRASSINOSTEROID-INSENSITIVE 1 (BRI1) and multiple key components of BR signaling directly or indirectly influence PM H+-ATPase activity. Secondly, the SMALL AUXIN UP RNA (SAUR) gene family physically interacts with BRI1 to enhance organ development of Arabidopsis by activating PM H+-ATPase. Thirdly, RNA-sequencing (RNA-seq) assays showed that the expression of some SAUR genes is upregulated under the light or sucrose conditions, which is related to the phosphorylation state of the penultimate residue of PM H+-ATPase in a time-course manner. In this review, we describe the structural and functional features of PM H+-ATPase and summarize recent progress towards understanding the regulatory mechanism of PM H+-ATPase by BRs, and briefly introduce how PM H+-ATPase activity is modulated by its own biterminal regions and the post-translational modifications.
Keyword ：
BAK1 BAK1 BIN2 BIN2 Brassinosteroids Brassinosteroids BRI1 BRI1 Plasma membrane H+-ATPase Plasma membrane H+-ATPase SAUR SAUR
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| GB/T 7714 | Lin, Zhaoheng , Zhu, Pan , Gao, Liyang et al. Recent Advances in Understanding the Regulatory Mechanism of Plasma Membrane H+-ATPase through the Brassinosteroid Signaling Pathway [J]. | PLANT AND CELL PHYSIOLOGY , 2024 , 65 (10) : 1515-1529 . |
| MLA | Lin, Zhaoheng et al. "Recent Advances in Understanding the Regulatory Mechanism of Plasma Membrane H+-ATPase through the Brassinosteroid Signaling Pathway" . | PLANT AND CELL PHYSIOLOGY 65 . 10 (2024) : 1515-1529 . |
| APA | Lin, Zhaoheng , Zhu, Pan , Gao, Liyang , Chen, Xuanyi , Li, Meijing , Wang, Yuhe et al. Recent Advances in Understanding the Regulatory Mechanism of Plasma Membrane H+-ATPase through the Brassinosteroid Signaling Pathway . | PLANT AND CELL PHYSIOLOGY , 2024 , 65 (10) , 1515-1529 . |
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The ancient guanine nucleotide-binding (G) proteins are a group of critical regulatory and signal transduction proteins, widely involved in diverse cellular processes of all kingdoms of life. YchF is a kind of universally conserved novel unconventional G protein that appears to be crucial for growth and stress response in eukaryotes and bacteria. YchF is able to bind and hydrolyze both adenine nucleoside triphosphate (ATP) and guanosine nucleoside triphosphate (GTP), unlike other members of the P-loop GTPases. Hence, it can transduce signals and mediate multiple biological functions by using either ATP or GTP. YchF is not only a nucleotide-dependent translational factor associated with the ribosomal particles and proteasomal subunits, potentially bridging protein biosynthesis and degradation, but also sensitive to reactive oxygen species (ROS), probably recruiting many partner proteins in response to environmental stress. In this review, we summarize the latest insights into how YchF is associated with protein translation and ubiquitin-dependent protein degradation to regulate growth and maintain proteostasis under stress conditions.
Keyword ：
growth growth P-loop NTPase P-loop NTPase protein degradation protein degradation protein translation protein translation proteosome proteosome ribosome ribosome stress response stress response YchF YchF
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| GB/T 7714 | Lin, Zhaoheng , Li, Rongfang , Han, Zhiwei et al. The Universally Conserved Unconventional G Protein YchF Is Critical for Growth and Stress Response [J]. | LIFE-BASEL , 2023 , 13 (4) . |
| MLA | Lin, Zhaoheng et al. "The Universally Conserved Unconventional G Protein YchF Is Critical for Growth and Stress Response" . | LIFE-BASEL 13 . 4 (2023) . |
| APA | Lin, Zhaoheng , Li, Rongfang , Han, Zhiwei , Liu, Yi , Gao, Liyang , Huang, Suchang et al. The Universally Conserved Unconventional G Protein YchF Is Critical for Growth and Stress Response . | LIFE-BASEL , 2023 , 13 (4) . |
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Root hydrotropism refers to root directional growth toward soil moisture. Cortical microtubule arrays are essential for determining the growth axis of the elongating cells in plants. However, the role of microtubule reorganization in root hydrotropism remains elusive. Here, we demonstrate that the well-ordered microtubule arrays and the microtubule-severing protein KATANIN (KTN) play important roles in regulating root hydrotropism in Arabidopsis. We found that the root hydrotropic bending of the ktn1 mutant was severely attenuated but not root gravitropism. After hydrostimulation, cortical microtubule arrays in cells of the elongation zone of wild-type (WT) Col-0 roots were reoriented from transverse into an oblique array along the axis of cell elongation, whereas the microtubule arrays in the ktn1 mutant remained in disorder. Moreover, we revealed that abscisic acid (ABA) signaling enhanced the root hydrotropism of WT and partially rescued the oryzalin (a microtubule destabilizer) alterative root hydrotropism of WT but not ktn1 mutants. These results suggest that katanin-dependent microtubule ordering is required for root hydrotropism, which might work downstream of ABA signaling pathways for plant roots to search for water.
Keyword ：
abscisic acid abscisic acid cortical microtubule arrays cortical microtubule arrays KATANIN KATANIN oryzalin oryzalin root hydrotropism root hydrotropism
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| GB/T 7714 | Miao, Rui , Siao, Wei , Zhang, Na et al. Katanin-Dependent Microtubule Ordering in Association with ABA Is Important for Root Hydrotropism [J]. | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES , 2022 , 23 (7) . |
| MLA | Miao, Rui et al. "Katanin-Dependent Microtubule Ordering in Association with ABA Is Important for Root Hydrotropism" . | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 23 . 7 (2022) . |
| APA | Miao, Rui , Siao, Wei , Zhang, Na , Lei, Zuliang , Lin, Deshu , Bhalerao, Rishikesh P. et al. Katanin-Dependent Microtubule Ordering in Association with ABA Is Important for Root Hydrotropism . | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES , 2022 , 23 (7) . |
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The enzyme activity of the plasma membrane (PM) proton pump, well known as arabidopsis PM H+-ATPase (AHA) in the model plant arabidopsis (Arabidopsis thaliana), is controlled by phosphorylation. Three different classes of phytohormones, brassinosteroids (BRs), abscisic acid (ABA), and auxin regulate plant growth and responses to environmental stimuli, at least in part by modulating the activity of the pump through phosphorylation of the penultimate Thr residue in its carboxyl terminus. Here, we review the current knowledge regarding this tripartite hormonal AHA regulation and highlight mechanisms of activation and deactivation, as well as the significance of hormonal crosstalk. Understanding the complexity of PM H+-ATPase regulation in plants might provide new strategies for sustainable agriculture.
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| GB/T 7714 | Miao, Rui , Russinova, Eugenia , Rodriguez, Pedro L. . Tripartite hormonal regulation of plasma membrane H plus -ATPase activity [J]. | TRENDS IN PLANT SCIENCE , 2022 , 27 (6) : 588-600 . |
| MLA | Miao, Rui et al. "Tripartite hormonal regulation of plasma membrane H plus -ATPase activity" . | TRENDS IN PLANT SCIENCE 27 . 6 (2022) : 588-600 . |
| APA | Miao, Rui , Russinova, Eugenia , Rodriguez, Pedro L. . Tripartite hormonal regulation of plasma membrane H plus -ATPase activity . | TRENDS IN PLANT SCIENCE , 2022 , 27 (6) , 588-600 . |
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Guanine nucleotide-binding (G) proteins, namely, phosphate-binding (P) loop GTPases, play a critical role in life processes among different species. Based on the structural characteristics, G proteins can be divided into heterotrimeric G proteins, small G proteins and multiple unique unconventional G proteins. The highly conserved unconventional G protein YchF is composed of a core G domain, an inserted coiled-coil domain, and a TGS domain from the N-terminus to the C-terminus. In this review, we compared the structural characteristics of the G domain in rice OsYchF1 with those of Rattus norvegicus heterotrimeric G protein alpha-subunit and human small G protein Ras-related G protein C and analyzed the binding modes of these G proteins with GTP or ATP by performing molecular dynamics simulations. In summary, it will provide new insights into the enormous diversity of biological function of G proteins.
Keyword ：
ATP ATP G proteins G proteins GTP GTP heterotrimeric G proteins heterotrimeric G proteins small G proteins small G proteins unconventional G proteins unconventional G proteins
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| GB/T 7714 | Luo, Maozhen , Han, Zhiwei , Huang, Guoye et al. Structural comparison of unconventional G protein YchF with heterotrimeric G protein and small G protein [J]. | PLANT SIGNALING & BEHAVIOR , 2022 , 17 (1) . |
| MLA | Luo, Maozhen et al. "Structural comparison of unconventional G protein YchF with heterotrimeric G protein and small G protein" . | PLANT SIGNALING & BEHAVIOR 17 . 1 (2022) . |
| APA | Luo, Maozhen , Han, Zhiwei , Huang, Guoye , Li, Rongfang , Liu, Yi , Lu, Junjie et al. Structural comparison of unconventional G protein YchF with heterotrimeric G protein and small G protein . | PLANT SIGNALING & BEHAVIOR , 2022 , 17 (1) . |
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Abscisic acid (ABA) is a critical hormone for plant survival upon water stress. In this study, a large-scale mutants of Arabidopsis ecotype Columbia-0 (Col-0) by ethyl methanesulfonate (EMS)-mutagenesis were generated, and an improved root elongation under water-stress 1 (irew1) mutant showing significantly enhanced root growth was isolated upon a water potential gradient assay. Then, irew1 and ABA-related mutants in Arabidopsis or tomato plants were observed under water potential gradient assay or water-deficient condition. ABA pathway, Ca2+ response and primary root (PR) elongation rate were monitored in addition to DNA- and RNA-Seq analyses. We found that based on phenotyping and transcriptional analyses, irew1 exhibited the enhanced PR growth, ABA and Ca2+ responses compared to wild-type subjected to water stress. Interestingly, exogenous Ca2+ application enhanced PR growth of irew1, ABA-biosynthesis deficient mutants in Arabidopsis and tomato plants in response to water potential gradients or water-deficient condition. In combination with other ABA-related mutants and pharmacological study, our results suggest that ABA is required for root elongation associated with Ca2+ influx in response to water stress.
Keyword ：
Abscisic acid Abscisic acid Arabidopsis thaliana Arabidopsis thaliana Calcium Calcium Root Root Tomato Tomato Water stress Water stress
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| GB/T 7714 | Yuan, Wei , Zhang, Qian , Li, Ying et al. Abscisic acid is required for root elongation associated with Ca2+ influx in response to water stress [J]. | PLANT PHYSIOLOGY AND BIOCHEMISTRY , 2021 , 169 : 127-137 . |
| MLA | Yuan, Wei et al. "Abscisic acid is required for root elongation associated with Ca2+ influx in response to water stress" . | PLANT PHYSIOLOGY AND BIOCHEMISTRY 169 (2021) : 127-137 . |
| APA | Yuan, Wei , Zhang, Qian , Li, Ying , Wang, Qianwen , Xu, Feiyun , Dang, Xiaolin et al. Abscisic acid is required for root elongation associated with Ca2+ influx in response to water stress . | PLANT PHYSIOLOGY AND BIOCHEMISTRY , 2021 , 169 , 127-137 . |
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The hab1-1abi1-2abi2-2pp2ca-1 quadruple mutant (Qabi2-2) seedlings lacking key negative regulators of ABA signaling, namely, clade A protein phosphatases type 2C (PP2Cs), show more apoplastic H+ efflux in roots and display an enhanced root growth under normal medium or water stress medium compared to the wild type. The presence of low ABA concentration (0.1 micromolar), inhibiting PP2C activity via monomeric ABA receptors, enhances root apoplastic H+ efflux and growth of the wild type, resembling the Qabi2-2 phenotype in normal medium. Qabi2-2 seedlings also demonstrate increased hydrotropism compared to the wild type in obliquely-oriented hydrotropic experimental system, and asymmetric H+ efflux in root elongation zone is crucial for root hydrotropism. Moreover, we reveal that Arabidopsis ABA-insensitive 1, a key PP2C in ABA signaling, interacts directly with the C terminus of Arabidopsis plasma membrane H+-dependent adenosine triphosphatase 2 (AHA2) and dephosphorylates its penultimate threonine residue (Thr(947)), whose dephosphorylation negatively regulates AHA2.
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| GB/T 7714 | Miao, Rui , Yuan, Wei , Wang, Yue et al. Low ABA concentration promotes root growth and hydrotropism through relief of ABA INSENSITIVE 1-mediated inhibition of plasma membrane H+-ATPase 2 [J]. | SCIENCE ADVANCES , 2021 , 7 (12) . |
| MLA | Miao, Rui et al. "Low ABA concentration promotes root growth and hydrotropism through relief of ABA INSENSITIVE 1-mediated inhibition of plasma membrane H+-ATPase 2" . | SCIENCE ADVANCES 7 . 12 (2021) . |
| APA | Miao, Rui , Yuan, Wei , Wang, Yue , Garcia-Maquilon, Irene , Dang, Xiaolin , Li, Ying et al. Low ABA concentration promotes root growth and hydrotropism through relief of ABA INSENSITIVE 1-mediated inhibition of plasma membrane H+-ATPase 2 . | SCIENCE ADVANCES , 2021 , 7 (12) . |
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Abstract ：
Abscisic acid (ABA) is a critical hormone for plant survival under water stress. In this study, large-scale mutants of the Arabidopsis ecotype Columbia-0 (Col-0) were generated by ethyl methanesulfonate (EMS)-mutagenesis, and an improved root elongation under water-stress 1 (irew1) mutant showing significantly enhanced root growth was isolated under a water potential gradient assay. Then, irew1 and ABA-related mutants in Arabidopsis or tomato plants were observed under water potential gradient assay or water-deficient conditions. ABA pathway, Ca2+ response, and primary root (PR) elongation rate were monitored in addition to DNA- and RNA-Seq analyses. We found that based on phenotyping and transcriptional analyses, irew1 exhibited enhanced PR growth, ABA, and Ca2+ responses, compared to wild type subjected to water stress. Interestingly, exogenous Ca2+ application enhanced PR growth of irew1, ABA-biosynthesis deficient mutants in Arabidopsis, and tomato plants, in response to water potential gradients or water-deficient conditions. In combination with other ABA-related mutants and pharmacological studies, our results suggest that ABA is required for root elongation associated with Ca2+ influx in response to water stress.
Keyword ：
abscisic acid abscisic acid Arabidopsis thaliana Arabidopsis thaliana calcium calcium root root tomato tomato water stress water stress
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| GB/T 7714 | Yuan, Wei , Zhang, Qian , Li, Ying et al. 被撤回的出版物: Abscisic Acid Is Required for Root Elongation Associated With Ca2+ Influx in Response to Water Stress (Retracted article. See vol. 12, 2021) [J]. | FRONTIERS IN PLANT SCIENCE , 2020 , 11 . |
| MLA | Yuan, Wei et al. "被撤回的出版物: Abscisic Acid Is Required for Root Elongation Associated With Ca2+ Influx in Response to Water Stress (Retracted article. See vol. 12, 2021)" . | FRONTIERS IN PLANT SCIENCE 11 (2020) . |
| APA | Yuan, Wei , Zhang, Qian , Li, Ying , Wang, Qianwen , Xu, Feiyun , Dang, Xiaolin et al. 被撤回的出版物: Abscisic Acid Is Required for Root Elongation Associated With Ca2+ Influx in Response to Water Stress (Retracted article. See vol. 12, 2021) . | FRONTIERS IN PLANT SCIENCE , 2020 , 11 . |
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