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学者姓名：翟俊文
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Abstract ：
为探索鹤顶兰属(Phaius Lour.)和虾脊兰属(Calanthe R. Br.)植物不同组合杂交后代无菌发芽育苗技术，研究以6个杂交组合所得的果荚为材料，采用不同激素组合的试验方法，探究杂交种子无菌萌发的最适培养基。结果表明：6-BA和NAA对多数鹤顶兰属植物为母本的杂交组合萌发率有显著促进作用，但具体的浓度会因植物材料的不同而存在差异。对于虾脊兰属植物为母本的杂交组合，均在1.0 mg/L 6-BA+0.2 mg/L NAA+1.0 mg/L KT培养基中萌发率最高。研究表明，种子萌发率受多因素影响，不同激素在不同父母本组合中表现差异，未来可根据这一结果优化培养基配方和生长调节剂使用，提高两属杂交种子萌发率。
Keyword ：
Calanthe R. Br. Calanthe R. Br. Phaius Lour. Phaius Lour. 无菌播种 无菌播种 杂交育种 杂交育种 虾脊兰属 虾脊兰属 鹤顶兰属 鹤顶兰属
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| GB/T 7714 | 张凤婷 , 周燕 , 陈伦妍 et al. 鹤顶兰属和虾脊兰属植物杂交种子无菌萌发 [J]. | 林业科技通讯 , 2025 , 5 (06) : 56-60 . |
| MLA | 张凤婷 et al. "鹤顶兰属和虾脊兰属植物杂交种子无菌萌发" . | 林业科技通讯 5 . 06 (2025) : 56-60 . |
| APA | 张凤婷 , 周燕 , 陈伦妍 , 时子迪 , 吴沙沙 , 翟俊文 . 鹤顶兰属和虾脊兰属植物杂交种子无菌萌发 . | 林业科技通讯 , 2025 , 5 (06) , 56-60 . |
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为建立猪血木(Euryodendron excelsum)高效扦插快繁体系，该研究以移栽3 a的猪血木组培苗的1 a生侧芽、2 a生侧枝不同节位及实生苗成年株1 a生侧芽为材料，研究基质种类和植物生长调节剂浸泡时间对插穗生根的影响。结果表明，插穗生根方式为混合生根型，组培幼苗的生根率显著高于成年株，蛭石:珍珠岩体积比为2:1时最适宜生根，经吲哚丁酸(IBA)处理后的插穗生根率远高于萘乙酸(NAA)处理。组培幼苗插穗经1 500 mg/L IBA浸泡20 min的生根率和平均根长最高，分别为96.67%和0.97 cm；成年株插穗经过2 000 mg/L IBA浸泡10 s的生根率最高，为34.44%，平均生根数为4.22，平均根长为0.53 cm。组培幼苗2 a生侧枝顶端1～3节的生根率最高，为46.67%。采用组培幼苗作为插穗母株，成功率高，可以提高繁殖效率，降低生产成本。
Keyword ：
不定根 不定根 扦插繁殖 扦插繁殖 猪血木 猪血木 组培苗 组培苗
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| GB/T 7714 | 刘心怡 , 曾晶珏 , 吴坤林 et al. 基质和植物生长调节剂对猪血木组培苗、实生苗插穗生根的效应 [J]. | 热带亚热带植物学报 , 2025 , 33 (04) : 390-398 . |
| MLA | 刘心怡 et al. "基质和植物生长调节剂对猪血木组培苗、实生苗插穗生根的效应" . | 热带亚热带植物学报 33 . 04 (2025) : 390-398 . |
| APA | 刘心怡 , 曾晶珏 , 吴坤林 , 房林 , 吴沙沙 , 翟俊文 et al. 基质和植物生长调节剂对猪血木组培苗、实生苗插穗生根的效应 . | 热带亚热带植物学报 , 2025 , 33 (04) , 390-398 . |
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Calanthe graciliflora, an orchid species endemic to China, is one of the most widely distributed members of the genus Calanthe, occupying the highest latitudinal range and exhibiting strong cold tolerance. These traits suggest key adaptations to diverse and extreme environments, making it an ideal model for studying plant responses to climate variability. Ecological niche models (ENMs) are powerful tools for simulating species' potential distributions across different time periods, thereby aiding biodiversity conservation. In this study, 75 filtered occurrence records of C. graciliflora and 19 climatic variables, derived from field surveys and herbarium records in China, were used to model the species' potential distribution across 6 periods (Last Interglacial, Last Glacial Maximum, Middle Holocene, Current, Future 2050s, and Future 2070s). Research findings indicate that key environmental factors influencing its distribution include mean diurnal temperature range (bio2), mean temperature of the warmest quarter (bio10), annual precipitation (bio12), and precipitation of the driest month (bio14). Historically, suitable habitats for C. graciliflora were primarily concentrated south of the Qinling-Huaihe River region, closely associated with the Qinling, Luoxiao, Nanling, and Mount Wuyi ranges. During the Last Glacial Maximum, extensive suitable habitats existed in southwestern China, subsequently contracting to refugia in the Qinling and Mount Wuyi areas, underscoring these regions as refugia for C. graciliflora. Future projections indicate an overall decline in suitable habitat, highlighting the significant impacts of global warming on its long-term survival. Notably, this study represents the first application of the Max-Ent model to infer historical refugia of C. graciliflora while simultaneously integrating analyses of its future distribution shifts. This work fills the gap in long-term climate response research for this species and evaluates the impacts of climate change on its distribution, providing valuable insights for its phylogeography and conservation practice. By further identifying core habitats and clarifying their climate sensitivity, the findings provide a basis for developing targeted conservation strategies that prioritize key ecological areas and mitigate the risk of habitat loss.
Keyword ：
Climate change Climate change geographical distribution geographical distribution MaxEnt model MaxEnt model
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| GB/T 7714 | Guo, Pingting , Lu, Aixian , Zheng, Jiahao et al. The past, present, and future distribution of Calanthe graciliflora: implications for conservation and phylogeography [J]. | NATURE CONSERVATION-BULGARIA , 2025 , (60) : 21-38 . |
| MLA | Guo, Pingting et al. "The past, present, and future distribution of Calanthe graciliflora: implications for conservation and phylogeography" . | NATURE CONSERVATION-BULGARIA 60 (2025) : 21-38 . |
| APA | Guo, Pingting , Lu, Aixian , Zheng, Jiahao , Chen, Lunyan , Wu, Shasha , Hu, Chao et al. The past, present, and future distribution of Calanthe graciliflora: implications for conservation and phylogeography . | NATURE CONSERVATION-BULGARIA , 2025 , (60) , 21-38 . |
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杂交兰双艺金龙（Cymbidium‘Shuangyi Jinlong’）是福建百秾生态科技有限公司从杂交兰栽培品种黄金龙的无性系变异单株中选育出的新品种，集花艺和叶艺于一身，发芽性好，易开花，抗性较强，有较高的市场需求。为提高杂交兰种苗繁殖效率，提升种苗品质，建立标准化生产体系，以双艺金龙的根状茎为材料，对其根状茎增殖与分化、生根壮苗、炼苗移栽等各阶段影响因素进行正交试验。结果表明：（1）适宜根状茎增殖生长的培养基是MS+1.00 mg/L NAA+0.10 mg/L 6-BA+20.00 g/L蔗糖+1.00 g/L活性炭+6.30 g/L琼脂+1.00 g/L蛋白胨，根状茎数量和鲜质量增殖系数分别为3.57和4.63。（2）适宜根状茎芽分化的培养基是1/2MS+1.50 mg/L NAA+0.90 mg/L TDZ+100.00 g/L香蕉泥+25.00 g/L蔗糖+6.30 g/L琼脂，芽分化率为97.78%，分化系数达6.87。（3）生根壮苗效果较好的培养基是1/2MS+1.00 mg/L IBA+100.00 g/L香蕉泥+0.17 g/L KH
Keyword ：
分化 分化 增殖 增殖 杂交兰 杂交兰 根状茎 根状茎 生根壮苗 生根壮苗
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| GB/T 7714 | 邓淑雯 , 吴悠 , 张华园 et al. 杂交兰双艺金龙根状茎增殖、分化及生根壮苗研究 [J]. | 热带作物学报 , 2025 , 46 (01) : 152-161 . |
| MLA | 邓淑雯 et al. "杂交兰双艺金龙根状茎增殖、分化及生根壮苗研究" . | 热带作物学报 46 . 01 (2025) : 152-161 . |
| APA | 邓淑雯 , 吴悠 , 张华园 , 李世星 , 林子润 , 陈南川 et al. 杂交兰双艺金龙根状茎增殖、分化及生根壮苗研究 . | 热带作物学报 , 2025 , 46 (01) , 152-161 . |
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Cymbidium ensifolium is a representative species of national orchids, which has both ornamental and economic value. Its high-quality hybrid variety 'Dongfang Honghe' is highly favoured for its lotus petal flower type and long flowering period. However, the shortage of substrate resources such as perlite and the low fertilizer utilization rate in traditional cultivation restrict its large-scale production. In this study, a three-factor and five-level quadratic regression universal rotation combination design was employed to investigate the effects of substrate ratio, microbial agents, and slow-release fertilizer on the growth of Cymbidium ensifolium 'Dongfang Honghe'. Analysis of variance revealed that T15 (Chinese fir block : perlite ratio of 3:4, Effective Microorganisms (EM), and 20 grains of Osmocote No. 601) significantly affected seedling height, leaf number, root parameters, leaf total nitrogen content, and total phosphorus content compared to the other treatments. Furthermore, the leaf count, along with the total nitrogen and total phosphorus content in the leaves was increased with the application of the EM and an appropriate quantity of slow-release fertilizer (20 grains of Osmocote No. 601). The total nitrogen content in the leaves was significantly reduced by the microbial agent Osmocote No. 601, while the interaction of the matrix ratio and Osmocote No. 601 significantly enhanced the total phosphorus content in the leaves. Therefore, T15 was the optimal combination for plant growth and development. This provides a theoretical foundation and efficient reproduction of orchids, as well as technical guidance for the management and cultivation of C. ensifolium.
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| GB/T 7714 | Li, Qingyuan , Li, Qingnue , Chen, Lu et al. Effects of slow-release fertilizer and microbial agents on the growth of Cymbidium ensifolium 'Dongfang Honghe' cultivated in substrate [J]. | TECHNOLOGY IN HORTICULTURE , 2025 , 5 . |
| MLA | Li, Qingyuan et al. "Effects of slow-release fertilizer and microbial agents on the growth of Cymbidium ensifolium 'Dongfang Honghe' cultivated in substrate" . | TECHNOLOGY IN HORTICULTURE 5 (2025) . |
| APA | Li, Qingyuan , Li, Qingnue , Chen, Lu , Deng, Shuwen , Wu, Tong , Zhang, Shengtao et al. Effects of slow-release fertilizer and microbial agents on the growth of Cymbidium ensifolium 'Dongfang Honghe' cultivated in substrate . | TECHNOLOGY IN HORTICULTURE , 2025 , 5 . |
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Oxalis triangularis 'Purpurea' is an ornamental plant that exhibits nyctinastic movement. However, the underlying mechanisms remain unclear. The nyctinastic movement of the leaflets is regulated by a motor organ termed the pulvinus, in which the flexor cells and extensor cells adjust their osmosis potential antagonistically to drive the opening or closure of the leaflets. This study investigates the factors essential for the nyctinastic movement in O. triangularis 'Purpurea'. The critical structural and subcellular changes of the pulvini that facilitate the opening or closing movement of leaflets were revealed by sectioning and scanning electron microscope (SEM). The dynamic and significant changes of phytohormones [Auxin (IAA), Abscisic acid (ABA), Cytokinin (cis-Zeatin, cZ and trans-Zeatin, tZ), Gibberellin (GA), Salicylic acid (SA) and 1-aminocyclopropane-1-carboxylate (ACC, the ethylene precursor)] were detected during the leaflets opening or closing, and their contributions to nyctinastic movement were determined by exogenous application. Furthermore, dynamic but opposite Ca2+ flux was observed in the flexor and extensor cells during leaflets opening or closure, and the Ca2+ channel blocker disrupted normal leaflets movement. Transcriptomic analysis of the pulvini revealed key differentially expressed genes (DEGs) during leaflets opening and closing, and these DEGs are enriched in the function categories of circadian clock, hormone signaling, and ion channels. Notably, the core circadian clock gene, OtLHY, was demonstrated to be required for the nyctinastic movement of O. triangularis 'Purpurea' by virus-induced gene silencing (VIGS). Based on these findings, we propose a regulatory network involving the circadian clock, phytohormones, and ion channels that coordinate the nyctinastic movement of O. triangularis 'Purpurea'.
Keyword ：
nyctinastic movement nyctinastic movement OtLHY OtLHY O. triangularis 'Purpurea' O. triangularis 'Purpurea' transcriptome sequencing transcriptome sequencing
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| GB/T 7714 | Tuo, Wanli , Wang, Xuexuan , Yang, Zirui et al. Unraveling the Nyctinastic movement of Oxalis triangularis 'Purpurea': the role of circadian clock, hormones, and Ca2+signaling [J]. | PLANT JOURNAL , 2025 , 123 (4) . |
| MLA | Tuo, Wanli et al. "Unraveling the Nyctinastic movement of Oxalis triangularis 'Purpurea': the role of circadian clock, hormones, and Ca2+signaling" . | PLANT JOURNAL 123 . 4 (2025) . |
| APA | Tuo, Wanli , Wang, Xuexuan , Yang, Zirui , Chen, Huifang , Zhai, Junwen , Feng, Baomin et al. Unraveling the Nyctinastic movement of Oxalis triangularis 'Purpurea': the role of circadian clock, hormones, and Ca2+signaling . | PLANT JOURNAL , 2025 , 123 (4) . |
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兰科是被子植物中最大的科之一，物种繁多，分布广泛，因其奇特的花型、丰富的花色和持久的花期，具有极高的观赏价值。兰科植物也是国际上贸易最多的花卉之一，具有极高的经济价值。其开花时间和花朵品质是影响兰科植物观赏价值的重要因素，但由于大部分兰科植物的自然花期与市场需求错位、开花整齐度低、开花品质不佳，极大地限制了兰花产业的发展。因此，如何调控兰花花期、提升兰花花朵品质对兰科植物的生产和应用具有重要意义。本文结合国内外研究对兰科植物的花芽分化进程、花期调控技术及开花相关的分子调控机理进行综述，总结出兰科植物花芽分化的特征、兰科植物花期调控相关基础研究与技术存在的问题、兰科植物相关的开花途径以及蝴蝶兰相关的开花机理与花期调控技术，提出兰科植物花期调控机理和花期调控技术具有潜在研究价值的方向，以期为兰科植物的花期调控研究、规模化生产和品质提升提供参考。
Keyword ：
兰科植物 兰科植物 分子机理 分子机理 花期调控 花期调控 花芽分化 花芽分化
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| GB/T 7714 | 陈露 , 李青女 , 郑佳桐 et al. 兰科植物花期调控技术及分子机理研究进展 [J]. | 植物遗传资源学报 , 2025 , 26 (01) : 1-13 . |
| MLA | 陈露 et al. "兰科植物花期调控技术及分子机理研究进展" . | 植物遗传资源学报 26 . 01 (2025) : 1-13 . |
| APA | 陈露 , 李青女 , 郑佳桐 , 王奕晴 , 翟俊文 , 吴沙沙 . 兰科植物花期调控技术及分子机理研究进展 . | 植物遗传资源学报 , 2025 , 26 (01) , 1-13 . |
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建兰东方红荷（Cymbidium ensifolium‘Dongfang Honghe’）作为建兰的栽培新品种，姿态飘逸，粉白分明，具有较高的园林应用价值。为深入了解建兰成花规律，生产出满足节假日市场需求的高品质花卉，以东方红荷为试验材料，阐述其成花过程中花芽和花发育形态变化进程，并研究膨大素（CPPU）对其花期和开花品质的影响。结果表明：（1）根据石蜡切片结果将花芽分化进程划分为6个时期（花原基诱导期、花序原基分化期、小花原基分化期、花萼原基分化期、花瓣原基分化期及合蕊柱和花粉块分化期）；（2）根据花序芽形态变化特点将花发育过程划分为6个时期（花序芽分化末期、苞片包裹期、花序伸长期、花苞着色期、花朵绽放期和花朵凋谢期）；（3）喷施膨大素和细胞分裂素可促使建兰花期提前约1个月，其中1 g/L膨大素+1∶1000细胞分裂素综合效果最佳，处理44 d进入花发育阶段，花期提前26.55 d，开花持续时间较对照延长1.89 d，每盆花芽增多0.54个，花葶直径增粗0.28 mm；推测膨大素通过刺激假鳞茎的细胞分裂，促使其膨大，进而为成花转变积累充足的营养物质。本研究通过划分东方红荷成花进程，为后续深入研究成花机理、确定取样时期和产业中预测开花时间提供关键信息；探究膨大素对东方红荷开花的影响，为建立精准的花期调控技术体系奠定理论依据。
Keyword ：
建兰 建兰 膨大素 膨大素 花发育 花发育 花芽分化 花芽分化
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| GB/T 7714 | 郑佳桐 , 陈露 , 郭娉婷 et al. 建兰成花形态变化及膨大素对其开花的影响 [J]. | 热带作物学报 , 2025 , 46 (10) : 2469-2480 . |
| MLA | 郑佳桐 et al. "建兰成花形态变化及膨大素对其开花的影响" . | 热带作物学报 46 . 10 (2025) : 2469-2480 . |
| APA | 郑佳桐 , 陈露 , 郭娉婷 , 吴沙沙 , 翟俊文 . 建兰成花形态变化及膨大素对其开花的影响 . | 热带作物学报 , 2025 , 46 (10) , 2469-2480 . |
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Cymbidium hybrids has the advantages of strong growth, large number of flowers, and high market share. However, most orchid cultivation has problems such as over-reliance on artificial experience cultivation, lack of scientific basis for water and fertilizer application, and low degree of precision and intelligent cultivation. Monitoring the levels of N, P, and K in plant leaves facilitates real-time assessment of nutritional status while guiding optimization fertilization, thereby enhancing fertilizer efficiency and plant growth quality. Nevertheless, conventional nutrient detection methods are invasive, time-consuming, and expertise-dependent, necessitating advanced non-destructive alternatives. This study explores hyperspectral sensing technology for non-destructive monitoring of N, P, and K in hybrid orchid under greenhouse conditions. Three water-fertilization regimes were applied at strategic growth stages of hybrid orchids, with spectral data collected across four seasons using a spectrometer and a hyperspectral imaging device. Through the analysis of 720 combinations of ten spectral preprocessing methods and four modeling approaches, an optimal algorithm combination was identified. This optimal combination was integrated with the Stable Competitive Adaptive Reweighted Sampling algorithm to select spectral feature bands. Predictive models were then constructed to estimate the contents of N, P, and K in the orchid leaves. The results showed the utilization of Stable Competitive Adaptive Reweighted Sampling method, between 14.2% and 25.0% of the feature wavelengths significantly reduced the root mean square error of the models. By incorporating these methodologies, the predictive model for leaf N, P, and K contents utilizing the hyperspectral imaging device achieved determination coefficients (R2) of 0.7395, 0.7213, and 0.7055, respectively, on the test sets. For the spectrometer-based models predicting leaf N, P, and K contents, the corresponding R2 values for the test sets reached 0.8708, 0.8747, and 0.8557. These results validate hyperspectral sensing as a robust tool for non-destructive nutrient monitoring. The findings of this study provide a theoretical basis and effective methodology for the accurate and non-destructive detection of nutrient content in orchid leaves through hyperspectral sensing technology.
Keyword ：
Hyperspectral imaging Hyperspectral imaging Leaf nutrient content Leaf nutrient content Orchid Orchid Prediction models Prediction models Spectrometer Spectrometer
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| GB/T 7714 | Deng, Shuwen , Chen, Lu , Li, Qingnv et al. Development of a nondestructive prediction model for nutrient content of orchid leaves using different hyperspectral sensors [J]. | COMPUTERS AND ELECTRONICS IN AGRICULTURE , 2025 , 237 . |
| MLA | Deng, Shuwen et al. "Development of a nondestructive prediction model for nutrient content of orchid leaves using different hyperspectral sensors" . | COMPUTERS AND ELECTRONICS IN AGRICULTURE 237 (2025) . |
| APA | Deng, Shuwen , Chen, Lu , Li, Qingnv , Feng, Yao , Li, Chengfeng , Li, Shuang et al. Development of a nondestructive prediction model for nutrient content of orchid leaves using different hyperspectral sensors . | COMPUTERS AND ELECTRONICS IN AGRICULTURE , 2025 , 237 . |
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BackgroundPhalaenopsis orchids, belonging to the Orchidaceae family, one of the largest groups of angiosperms, possess significant commercial value due to their fascinating flowers. Petal size is a vital trait that directly determines flower size and shape of Phalaenopsis. However, the genetic and developmental regulation of petal size in Phalaenopsis remains unexplored.ResultsIn this study, we tracked the petal growth pattern through five stages of flower opening, discovering that cell division stops at stage 2 and stages 3 to 5 are the critical periods of rapid petal expansion. RNA-seq was then conducted to further reveal the molecular mechanisms underlying petal size regulation. Gene ontology (GO) analysis indicated that the differentially expressed genes (DEGs) from the four comparable groups were both enriched in terms related to cell expansion. Endogenous hormone assays showed that auxin, cytokinin, and gibberellin were implicated in the petal growth of Phalaenopsis. Moreover, six auxin signaling pathway genes, 11 cell expansion-related genes, and 30 transcription factors (TFs) identified through trend analysis were abundantly expressed during the critical period of petal expansion, suggesting that they may influence petal size by regulating cell expansion. In contrast, 18 TFs exhibited the highest expression levels at the S1 stage, indicating their potential role in petal cell proliferation. Based on weighted gene co-expression network analysis (WGCNA), six hub genes (PaLOF2, PaSWEET11, PaVNI2, PaHDA3, PaPMEI3, PaXTH30) were screened from the green and yellow module which was highly associated with the petal size.ConclusionOur results lay a foundation for further exploration of the molecular mechanisms regulating petal size development and are significant for molecular breeding programs aimed at generating novel Phalaenopsis with desirable traits.
Keyword ：
Cell division Cell division Cell expansion Cell expansion Differentially expressed genes Differentially expressed genes Petal growth Petal growth Phalaenopsis Phalaenopsis RNA-seq RNA-seq
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| GB/T 7714 | Guan, Yunxiao , Zhang, Qiaoyu , Zhang, Ting et al. Transcriptome analysis reveals the mechanisms underlying petal growth during the flower opening process in Phalaenopsis orchids [J]. | BMC PLANT BIOLOGY , 2025 , 25 (1) . |
| MLA | Guan, Yunxiao et al. "Transcriptome analysis reveals the mechanisms underlying petal growth during the flower opening process in Phalaenopsis orchids" . | BMC PLANT BIOLOGY 25 . 1 (2025) . |
| APA | Guan, Yunxiao , Zhang, Qiaoyu , Zhang, Ting , Li, Minghe , Ai, Ye , Zhai, Junwen et al. Transcriptome analysis reveals the mechanisms underlying petal growth during the flower opening process in Phalaenopsis orchids . | BMC PLANT BIOLOGY , 2025 , 25 (1) . |
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