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学者姓名：吴沙沙
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Abstract ：
为探索鹤顶兰属(Phaius Lour.)和虾脊兰属(Calanthe R. Br.)植物不同组合杂交后代无菌发芽育苗技术，研究以6个杂交组合所得的果荚为材料，采用不同激素组合的试验方法，探究杂交种子无菌萌发的最适培养基。结果表明：6-BA和NAA对多数鹤顶兰属植物为母本的杂交组合萌发率有显著促进作用，但具体的浓度会因植物材料的不同而存在差异。对于虾脊兰属植物为母本的杂交组合，均在1.0 mg/L 6-BA+0.2 mg/L NAA+1.0 mg/L KT培养基中萌发率最高。研究表明，种子萌发率受多因素影响，不同激素在不同父母本组合中表现差异，未来可根据这一结果优化培养基配方和生长调节剂使用，提高两属杂交种子萌发率。
Keyword ：
Calanthe R. Br. Calanthe R. Br. Phaius Lour. Phaius Lour. 无菌播种 无菌播种 杂交育种 杂交育种 虾脊兰属 虾脊兰属 鹤顶兰属 鹤顶兰属
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| GB/T 7714 | 张凤婷 , 周燕 , 陈伦妍 et al. 鹤顶兰属和虾脊兰属植物杂交种子无菌萌发 [J]. | 林业科技通讯 , 2025 , 5 (06) : 56-60 . |
| MLA | 张凤婷 et al. "鹤顶兰属和虾脊兰属植物杂交种子无菌萌发" . | 林业科技通讯 5 . 06 (2025) : 56-60 . |
| APA | 张凤婷 , 周燕 , 陈伦妍 , 时子迪 , 吴沙沙 , 翟俊文 . 鹤顶兰属和虾脊兰属植物杂交种子无菌萌发 . | 林业科技通讯 , 2025 , 5 (06) , 56-60 . |
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杂交兰双艺金龙（Cymbidium‘Shuangyi Jinlong’）是福建百秾生态科技有限公司从杂交兰栽培品种黄金龙的无性系变异单株中选育出的新品种，集花艺和叶艺于一身，发芽性好，易开花，抗性较强，有较高的市场需求。为提高杂交兰种苗繁殖效率，提升种苗品质，建立标准化生产体系，以双艺金龙的根状茎为材料，对其根状茎增殖与分化、生根壮苗、炼苗移栽等各阶段影响因素进行正交试验。结果表明：（1）适宜根状茎增殖生长的培养基是MS+1.00 mg/L NAA+0.10 mg/L 6-BA+20.00 g/L蔗糖+1.00 g/L活性炭+6.30 g/L琼脂+1.00 g/L蛋白胨，根状茎数量和鲜质量增殖系数分别为3.57和4.63。（2）适宜根状茎芽分化的培养基是1/2MS+1.50 mg/L NAA+0.90 mg/L TDZ+100.00 g/L香蕉泥+25.00 g/L蔗糖+6.30 g/L琼脂，芽分化率为97.78%，分化系数达6.87。（3）生根壮苗效果较好的培养基是1/2MS+1.00 mg/L IBA+100.00 g/L香蕉泥+0.17 g/L KH
Keyword ：
分化 分化 增殖 增殖 杂交兰 杂交兰 根状茎 根状茎 生根壮苗 生根壮苗
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| GB/T 7714 | 邓淑雯 , 吴悠 , 张华园 et al. 杂交兰双艺金龙根状茎增殖、分化及生根壮苗研究 [J]. | 热带作物学报 , 2025 , 46 (01) : 152-161 . |
| MLA | 邓淑雯 et al. "杂交兰双艺金龙根状茎增殖、分化及生根壮苗研究" . | 热带作物学报 46 . 01 (2025) : 152-161 . |
| APA | 邓淑雯 , 吴悠 , 张华园 , 李世星 , 林子润 , 陈南川 et al. 杂交兰双艺金龙根状茎增殖、分化及生根壮苗研究 . | 热带作物学报 , 2025 , 46 (01) , 152-161 . |
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Oxalis vulcanicola 'Sunset Velvet', a cultivar of Oxalidaceae, is a perennial dicot known for its compact growth, vibrant foliage, and attractive flowers, making it a popular choice for ornamental potted plants. Despite its ornamental value and physiological significance, significant challenges remain in gene function validation and genetic transformation due to the lack of a well-established regeneration system. This study aimed to establish an effective tissue culture system for O. vulcanicola 'Sunset Velvet' to facilitate genetic transformation and large-scale propagation. Sterile stem segments with buds were used explants, and a direct shoot organogenesis pathway was employed to initiate the culture system. Optimization of hormone combinations led to selection of suitable induction, proliferation, and rooting media. The most effective disinfection protocol was a 2-min treatment with 75% ethanol, followed by a 15-min treatment with 3% sodium hypochlorite. The optimal medium for inducing multiple shoot formation consisted of MS + 0.50 mgL-1 NAA 1.00 mgL-1 6-BA + 30 gL-1 sucrose + 5 gL-1 agar, achieving an induction rate of 74.45 +/- 6.94% and the longest shoot length of 0.99 +/- 0.34 cm after 30 culture. For shoot proliferation, MS + 0.50 mgL-1 NAA + 1.00 mgL-1 6-BA + 30 gL-1 sucrose + 5 gL-1 agar achieved a multiplication rate of 20.32 +/- 0.54-fold, with a browning rate of 18.65 +/- 0.32%. When 0.01 mgL-1 IBA was added to the medium, the multiplication rate decreased slightly to 17.39 +/- 0.70-fold the browning rate reduced to 10.21 +/- 0.33%. For rooting, the best medium was 1/2 MS + 0.10 mgL-1 NAA + 30 gL-1 sucrose + 5 gL-1 agar, resulting average of 14.10 roots per plant, with an average root length of 2.73 +/- 0.15 cm and plant height of 4.26 +/- 0.05 cm, starting from stem segments with an initial height of 3 cm. These findings provide important technical support for the large-scale propagation and genetic transformation of O. vulcanicola 'Sunset Velvet'.
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| GB/T 7714 | Wang, Xuexuan , Tuo, Wanli , Zhang, Shengtao et al. Efficient plant regeneration through direct shoot organogenesis of Oxalis vulcanicola 'Sunset Velvet' [J]. | TECHNOLOGY IN HORTICULTURE , 2025 , 5 . |
| MLA | Wang, Xuexuan et al. "Efficient plant regeneration through direct shoot organogenesis of Oxalis vulcanicola 'Sunset Velvet'" . | TECHNOLOGY IN HORTICULTURE 5 (2025) . |
| APA | Wang, Xuexuan , Tuo, Wanli , Zhang, Shengtao , Wu, Tong , Li, Jikang , Zhai, Junwen et al. Efficient plant regeneration through direct shoot organogenesis of Oxalis vulcanicola 'Sunset Velvet' . | TECHNOLOGY IN HORTICULTURE , 2025 , 5 . |
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Cymbidium ensifolium is a representative species of national orchids, which has both ornamental and economic value. Its high-quality hybrid variety 'Dongfang Honghe' is highly favoured for its lotus petal flower type and long flowering period. However, the shortage of substrate resources such as perlite and the low fertilizer utilization rate in traditional cultivation restrict its large-scale production. In this study, a three-factor and five-level quadratic regression universal rotation combination design was employed to investigate the effects of substrate ratio, microbial agents, and slow-release fertilizer on the growth of Cymbidium ensifolium 'Dongfang Honghe'. Analysis of variance revealed that T15 (Chinese fir block : perlite ratio of 3:4, Effective Microorganisms (EM), and 20 grains of Osmocote No. 601) significantly affected seedling height, leaf number, root parameters, leaf total nitrogen content, and total phosphorus content compared to the other treatments. Furthermore, the leaf count, along with the total nitrogen and total phosphorus content in the leaves was increased with the application of the EM and an appropriate quantity of slow-release fertilizer (20 grains of Osmocote No. 601). The total nitrogen content in the leaves was significantly reduced by the microbial agent Osmocote No. 601, while the interaction of the matrix ratio and Osmocote No. 601 significantly enhanced the total phosphorus content in the leaves. Therefore, T15 was the optimal combination for plant growth and development. This provides a theoretical foundation and efficient reproduction of orchids, as well as technical guidance for the management and cultivation of C. ensifolium.
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| GB/T 7714 | Li, Qingyuan , Li, Qingnue , Chen, Lu et al. Effects of slow-release fertilizer and microbial agents on the growth of Cymbidium ensifolium 'Dongfang Honghe' cultivated in substrate [J]. | TECHNOLOGY IN HORTICULTURE , 2025 , 5 . |
| MLA | Li, Qingyuan et al. "Effects of slow-release fertilizer and microbial agents on the growth of Cymbidium ensifolium 'Dongfang Honghe' cultivated in substrate" . | TECHNOLOGY IN HORTICULTURE 5 (2025) . |
| APA | Li, Qingyuan , Li, Qingnue , Chen, Lu , Deng, Shuwen , Wu, Tong , Zhang, Shengtao et al. Effects of slow-release fertilizer and microbial agents on the growth of Cymbidium ensifolium 'Dongfang Honghe' cultivated in substrate . | TECHNOLOGY IN HORTICULTURE , 2025 , 5 . |
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Pleione maculata (Lindl.) Lindl. is an epiphytic herb of the genus Pleione D. Don, Orchidaceae, which has important ornamental, medicinal, economic, and ecological values, and its market demand is increasing. However, the lack of a system for seed breeding technology severely limits the production of large-scale cultivation of this species. The purpose of this study was to optimize the medium and to study the effects of basic medium types, plant growth regulators, natural additives, and sucrose concentration on seed germination and protocorm-like body (PLB) proliferation and differentiation. In this study, the highest percentage of seed germination was observed on B5 + 1.0 mg/L NAA + 20 g/L glucose + 1 g/L protein + 20 g/L potato homogenate (PH) + 1 g/L activated carbon (AC) + 6 g/L agar (pH 5.7-5.8), which reached 86.26%. The best medium type for protocorm proliferation was 1/2 MS + 1 mg/L NAA + 1.0 mg/L 6-BA + 20 g/L sucrose + 100 g/L PH + 1 g/L protein + 1 g/L activated charcoal + 5.8 g/L agar (pH 5.7-5.8), which multiplication factor was 3.20. The best media for protocorm differentiation was 1/2 MS + 0.5 mg/L NAA + 1.0 mg/L 6-BA + 100 mL/L CW + 20 g/L sucrose + 1 g/L protein + 1 g/L activated charcoal + 5.8 g/L agar (pH 5.7-5.8), which multiplication factor was 5.77. Therefore, by adjusting the type of medium, plant growth regulators, and sucrose concentration, the aseptic germination and protocorm differentiation of P. maculata seeds can be achieved.
Keyword ：
differentiation differentiation Pleione maculata Pleione maculata protocorm protocorm seed germination seed germination
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| GB/T 7714 | Wu, Kefei , Yuan, Ying , Xu, Lianhuan et al. Effects of Basic Medium Types and Plant Growth Regulators on Seed Germination and Protocorm Differentiation of Pleione maculata (Lindl.) Lindl. (Orchidaceae) [J]. | BIOLOGY BULLETIN , 2025 , 52 (2) . |
| MLA | Wu, Kefei et al. "Effects of Basic Medium Types and Plant Growth Regulators on Seed Germination and Protocorm Differentiation of Pleione maculata (Lindl.) Lindl. (Orchidaceae)" . | BIOLOGY BULLETIN 52 . 2 (2025) . |
| APA | Wu, Kefei , Yuan, Ying , Xu, Lianhuan , Yang, Zirui , Chen, Siyu , Cai, Yijing et al. Effects of Basic Medium Types and Plant Growth Regulators on Seed Germination and Protocorm Differentiation of Pleione maculata (Lindl.) Lindl. (Orchidaceae) . | BIOLOGY BULLETIN , 2025 , 52 (2) . |
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Cycads, renowned as "living fossils", are among the most ancient extant seed plants, playing a crucial role in understanding plant evolution and sex differentiation. Despite their importance, research on their genetics and sex differentiation remains scarce. This study investigates three species, represented by six samples, collected from various regions in Fujian Province, China, using whole-genome resequencing on the Illumina platform. The sequence data underwent rigorous quality control, alignment, and variant detection, focusing on SNP and InDel distribution and annotation. Among the studied species, Cycas revoluta exhibited the highest number of SNPs and the greatest heterozygosity values. Based on SNP data, phylogenetic trees and principal component analysis revealed distinct clusters, with the three C. revoluta samples forming one cluster, while the two C. szechuanensis samples and the C. taiwaniana sample were grouped separately. Gene function using COG and GO annotations, and KEGG enrichment analysis, all highlighted differences in genomic structure and functional gene distribution between male and female cycads. Notably, genes associated with sex differentiation, such as MADS-box and auxin-responsive protein genes, were shown, while other transcription factors showed distinct annotations and enrichment patterns based on sex. This study improves our understanding of genetic variation, evolutionary relationships, and gene enrichment in cycads, providing a foundation for conservation, cultivation, and insights into sex differentiation mechanisms in these ancient plants.
Keyword ：
genetic diversity genetic diversity InDel InDel sex differentiation sex differentiation SNP SNP
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| GB/T 7714 | Xu, Xinyu , A. El-Kassaby, Yousry , Liu, Sijia et al. Whole-Genome Resequencing Reveals Phylogenetic Relationships and Sex Differentiation Mechanisms Among Fujian Cycas Species [J]. | HORTICULTURAE , 2025 , 11 (5) . |
| MLA | Xu, Xinyu et al. "Whole-Genome Resequencing Reveals Phylogenetic Relationships and Sex Differentiation Mechanisms Among Fujian Cycas Species" . | HORTICULTURAE 11 . 5 (2025) . |
| APA | Xu, Xinyu , A. El-Kassaby, Yousry , Liu, Sijia , Zhang, Juan , Zhang, Lanqi , Li, Junnan et al. Whole-Genome Resequencing Reveals Phylogenetic Relationships and Sex Differentiation Mechanisms Among Fujian Cycas Species . | HORTICULTURAE , 2025 , 11 (5) . |
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Calanthe graciliflora, an orchid species endemic to China, is one of the most widely distributed members of the genus Calanthe, occupying the highest latitudinal range and exhibiting strong cold tolerance. These traits suggest key adaptations to diverse and extreme environments, making it an ideal model for studying plant responses to climate variability. Ecological niche models (ENMs) are powerful tools for simulating species' potential distributions across different time periods, thereby aiding biodiversity conservation. In this study, 75 filtered occurrence records of C. graciliflora and 19 climatic variables, derived from field surveys and herbarium records in China, were used to model the species' potential distribution across 6 periods (Last Interglacial, Last Glacial Maximum, Middle Holocene, Current, Future 2050s, and Future 2070s). Research findings indicate that key environmental factors influencing its distribution include mean diurnal temperature range (bio2), mean temperature of the warmest quarter (bio10), annual precipitation (bio12), and precipitation of the driest month (bio14). Historically, suitable habitats for C. graciliflora were primarily concentrated south of the Qinling-Huaihe River region, closely associated with the Qinling, Luoxiao, Nanling, and Mount Wuyi ranges. During the Last Glacial Maximum, extensive suitable habitats existed in southwestern China, subsequently contracting to refugia in the Qinling and Mount Wuyi areas, underscoring these regions as refugia for C. graciliflora. Future projections indicate an overall decline in suitable habitat, highlighting the significant impacts of global warming on its long-term survival. Notably, this study represents the first application of the Max-Ent model to infer historical refugia of C. graciliflora while simultaneously integrating analyses of its future distribution shifts. This work fills the gap in long-term climate response research for this species and evaluates the impacts of climate change on its distribution, providing valuable insights for its phylogeography and conservation practice. By further identifying core habitats and clarifying their climate sensitivity, the findings provide a basis for developing targeted conservation strategies that prioritize key ecological areas and mitigate the risk of habitat loss.
Keyword ：
Climate change Climate change geographical distribution geographical distribution MaxEnt model MaxEnt model
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| GB/T 7714 | Guo, Pingting , Lu, Aixian , Zheng, Jiahao et al. The past, present, and future distribution of Calanthe graciliflora: implications for conservation and phylogeography [J]. | NATURE CONSERVATION-BULGARIA , 2025 , (60) : 21-38 . |
| MLA | Guo, Pingting et al. "The past, present, and future distribution of Calanthe graciliflora: implications for conservation and phylogeography" . | NATURE CONSERVATION-BULGARIA 60 (2025) : 21-38 . |
| APA | Guo, Pingting , Lu, Aixian , Zheng, Jiahao , Chen, Lunyan , Wu, Shasha , Hu, Chao et al. The past, present, and future distribution of Calanthe graciliflora: implications for conservation and phylogeography . | NATURE CONSERVATION-BULGARIA , 2025 , (60) , 21-38 . |
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BackgroundPhalaenopsis orchids, belonging to the Orchidaceae family, one of the largest groups of angiosperms, possess significant commercial value due to their fascinating flowers. Petal size is a vital trait that directly determines flower size and shape of Phalaenopsis. However, the genetic and developmental regulation of petal size in Phalaenopsis remains unexplored.ResultsIn this study, we tracked the petal growth pattern through five stages of flower opening, discovering that cell division stops at stage 2 and stages 3 to 5 are the critical periods of rapid petal expansion. RNA-seq was then conducted to further reveal the molecular mechanisms underlying petal size regulation. Gene ontology (GO) analysis indicated that the differentially expressed genes (DEGs) from the four comparable groups were both enriched in terms related to cell expansion. Endogenous hormone assays showed that auxin, cytokinin, and gibberellin were implicated in the petal growth of Phalaenopsis. Moreover, six auxin signaling pathway genes, 11 cell expansion-related genes, and 30 transcription factors (TFs) identified through trend analysis were abundantly expressed during the critical period of petal expansion, suggesting that they may influence petal size by regulating cell expansion. In contrast, 18 TFs exhibited the highest expression levels at the S1 stage, indicating their potential role in petal cell proliferation. Based on weighted gene co-expression network analysis (WGCNA), six hub genes (PaLOF2, PaSWEET11, PaVNI2, PaHDA3, PaPMEI3, PaXTH30) were screened from the green and yellow module which was highly associated with the petal size.ConclusionOur results lay a foundation for further exploration of the molecular mechanisms regulating petal size development and are significant for molecular breeding programs aimed at generating novel Phalaenopsis with desirable traits.
Keyword ：
Cell division Cell division Cell expansion Cell expansion Differentially expressed genes Differentially expressed genes Petal growth Petal growth Phalaenopsis Phalaenopsis RNA-seq RNA-seq
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| GB/T 7714 | Guan, Yunxiao , Zhang, Qiaoyu , Zhang, Ting et al. Transcriptome analysis reveals the mechanisms underlying petal growth during the flower opening process in Phalaenopsis orchids [J]. | BMC PLANT BIOLOGY , 2025 , 25 (1) . |
| MLA | Guan, Yunxiao et al. "Transcriptome analysis reveals the mechanisms underlying petal growth during the flower opening process in Phalaenopsis orchids" . | BMC PLANT BIOLOGY 25 . 1 (2025) . |
| APA | Guan, Yunxiao , Zhang, Qiaoyu , Zhang, Ting , Li, Minghe , Ai, Ye , Zhai, Junwen et al. Transcriptome analysis reveals the mechanisms underlying petal growth during the flower opening process in Phalaenopsis orchids . | BMC PLANT BIOLOGY , 2025 , 25 (1) . |
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建兰东方红荷（Cymbidium ensifolium‘Dongfang Honghe’）作为建兰的栽培新品种，姿态飘逸，粉白分明，具有较高的园林应用价值。为深入了解建兰成花规律，生产出满足节假日市场需求的高品质花卉，以东方红荷为试验材料，阐述其成花过程中花芽和花发育形态变化进程，并研究膨大素（CPPU）对其花期和开花品质的影响。结果表明：（1）根据石蜡切片结果将花芽分化进程划分为6个时期（花原基诱导期、花序原基分化期、小花原基分化期、花萼原基分化期、花瓣原基分化期及合蕊柱和花粉块分化期）；（2）根据花序芽形态变化特点将花发育过程划分为6个时期（花序芽分化末期、苞片包裹期、花序伸长期、花苞着色期、花朵绽放期和花朵凋谢期）；（3）喷施膨大素和细胞分裂素可促使建兰花期提前约1个月，其中1 g/L膨大素+1∶1000细胞分裂素综合效果最佳，处理44 d进入花发育阶段，花期提前26.55 d，开花持续时间较对照延长1.89 d，每盆花芽增多0.54个，花葶直径增粗0.28 mm；推测膨大素通过刺激假鳞茎的细胞分裂，促使其膨大，进而为成花转变积累充足的营养物质。本研究通过划分东方红荷成花进程，为后续深入研究成花机理、确定取样时期和产业中预测开花时间提供关键信息；探究膨大素对东方红荷开花的影响，为建立精准的花期调控技术体系奠定理论依据。
Keyword ：
建兰 建兰 膨大素 膨大素 花发育 花发育 花芽分化 花芽分化
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| GB/T 7714 | 郑佳桐 , 陈露 , 郭娉婷 et al. 建兰成花形态变化及膨大素对其开花的影响 [J]. | 热带作物学报 , 2025 , 46 (10) : 2469-2480 . |
| MLA | 郑佳桐 et al. "建兰成花形态变化及膨大素对其开花的影响" . | 热带作物学报 46 . 10 (2025) : 2469-2480 . |
| APA | 郑佳桐 , 陈露 , 郭娉婷 , 吴沙沙 , 翟俊文 . 建兰成花形态变化及膨大素对其开花的影响 . | 热带作物学报 , 2025 , 46 (10) , 2469-2480 . |
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Cymbidium ensifolium is recognized as one of the most significant and widely cultivated ornamental plants within the Orchidaceae family. Its flowering period extends from June to September, out of alignment with China's National Day, New Year's Day, the Spring Festival, and other significant holidays, which poses challenges for the industry's development. This study examined the growth rhythm of three-year-old C. ensifolium 'Dongfang Honghe' and determined that, without intervention, flower bud differentiation typically occurs from late April to early June, flower development from late May to mid-July, reaching peak flowering in mid-July and concluding by late August. The research further investigated the regulation of the flowering period of 'Dongfang Honghe', through temperature manipulation and the application of plant growth regulators. It was observed that decreasing treatment temperature resulted in a continuous delay of flowering phase, and varying concentrations of 6-Benzylaminopurine (6-BA) produced significant differences in both flowering time and flower quality. Additionally, through the application of temperature treatments and 6-BA, is feasible to achieve flowering on Labor Day, National Day, and New Year's Day. Finally, utilizing transcriptome data from various stages of flower buds development in 'Dongfang Honghe', an identification and expression analysis of flowering-related genes was conducted. Preliminary findings suggest the involvement of three distinct pathways in the induction of flowering, facilitating the transition from vegetative to reproductive growth: the environmental temperature pathway, the heat stress pathway, and the gibberellin pathway. This study systematically explores flowering period control techniques based on the growth rhythm of 'Dongfang Honghe' and analyzes the regulatory pathways of flower induction, thereby establishing a foundation for the development of a flowering regulation technique system for C. ensifolium.
Keyword ：
Cymbidium ensifolium Cymbidium ensifolium Flower bud differentiation Flower bud differentiation Flower development Flower development Flowering regulation Flowering regulation Growth rhythm Growth rhythm
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| GB/T 7714 | Chen, Lu , Zheng, Jiatong , Wang, Fuhua et al. The Effects of Temperature and 6-Benzylaminopurine on the Flowering of Cymbidium ensifolium 'Dongfang Honghe' [J]. | JOURNAL OF PLANT GROWTH REGULATION , 2025 . |
| MLA | Chen, Lu et al. "The Effects of Temperature and 6-Benzylaminopurine on the Flowering of Cymbidium ensifolium 'Dongfang Honghe'" . | JOURNAL OF PLANT GROWTH REGULATION (2025) . |
| APA | Chen, Lu , Zheng, Jiatong , Wang, Fuhua , Wu, Tong , Li, Qingnv , Deng, Shuwen et al. The Effects of Temperature and 6-Benzylaminopurine on the Flowering of Cymbidium ensifolium 'Dongfang Honghe' . | JOURNAL OF PLANT GROWTH REGULATION , 2025 . |
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