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学者姓名：陈吉龙
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Abstract ：
H3N3 avian influenza viruses (AIVs) are less prevalent in poultry than H3N8 viruses. However, although relatively rare, reassortant H3N3 viruses have been known to appear in both domestic poultry and wild birds. In this study, we isolated the H3N3 virus in chickens sourced from a live poultry market in China. A comprehensive genomic analysis revealed that the virus possessed a single basic amino acid in the cleavage site of the hemagglutinin (HA) gene. Phylogenetic analysis indicated that eight genes in the H3N3 virus belong to the Eurasian lineage. Specifically, the HA and NA genes were clustered with H3N2 and H11N3, respectively, while the internal genes were closely related to the H3N8 and H9N2 viruses. Furthermore, the H3N3 virus exhibited high and moderate stability in thermal and acidic conditions and efficient replication capabilities in mammalian cells. The H3N3 virus demonstrated that it could infect and replicate in the upper and lower respiratory tract of BALB/c mice without prior adaptation, triggering hemagglutination inhibition (HI) antibody titres ranging from 80 to 160; notably, the H3N3 virus replicated vigorously within the chicken respiratory and digestive tracts. The virus also transmitted efficiently and swiftly among chickens through direct contact, leading to higher levels of HI antibodies in both the inoculated and contact birds. These findings suggest that the H3N3 virus may be a novel reassortant originating from viruses circulating in domestic poultry, thus demonstrating an increased pathogenicity and transmissibility in chickens. Our study determines that H3N3 AIV potentially threatens the poultry industry and public health, highlighting the importance of active surveillance of AIVs.
Keyword ：
avian influenza virus avian influenza virus evolution evolution pathogenicity pathogenicity transmissibility transmissibility Zoonoses Zoonoses
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| GB/T 7714 | Zhang, Chunping , Zhao, Conghui , Huang, Jiacheng et al. Emergence of a novel reassortant H3N3 avian influenza virus with enhanced pathogenicity and transmissibility in chickens in China [J]. | VETERINARY RESEARCH , 2025 , 56 (1) . |
| MLA | Zhang, Chunping et al. "Emergence of a novel reassortant H3N3 avian influenza virus with enhanced pathogenicity and transmissibility in chickens in China" . | VETERINARY RESEARCH 56 . 1 (2025) . |
| APA | Zhang, Chunping , Zhao, Conghui , Huang, Jiacheng , Wang, Yang , Jiang, Bo , Zheng, Hangyu et al. Emergence of a novel reassortant H3N3 avian influenza virus with enhanced pathogenicity and transmissibility in chickens in China . | VETERINARY RESEARCH , 2025 , 56 (1) . |
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Abstract ：
随着翻译组测序、多肽组学和生物信息学分析等技术的快速发展，人们发现大量长链非编码RNA(long non-coding RNA, lncRNA)含有可编码功能性微肽的短开放阅读框。这些由lncRNA编码的微肽不仅参与调控骨骼肌的生理功能、胚胎发育和神经细胞发育，还对多种癌症的发生与发展、炎症反应及心血管疾病产生影响。研究这些微肽的功能对于深入理解生命活动的调控机制具有重要的科学意义，也为相关疾病的防治提供新的思路与方向。概述了鉴定lncRNA编码微肽的常用方法，以及这些微肽功能的最新研究进展，以期为后续研究提供参考。
Keyword ：
功能 功能 微肽 微肽 短开放阅读框 短开放阅读框 鉴定 鉴定 长链非编码RNA 长链非编码RNA
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| GB/T 7714 | 黄桂英 , 王李伟 , 刘嘉茵 et al. 长链非编码RNA编码微肽的鉴定和功能研究进展 [J]. | 福建农林大学学报(自然科学版) , 2025 , 54 (01) : 89-99 . |
| MLA | 黄桂英 et al. "长链非编码RNA编码微肽的鉴定和功能研究进展" . | 福建农林大学学报(自然科学版) 54 . 01 (2025) : 89-99 . |
| APA | 黄桂英 , 王李伟 , 刘嘉茵 , 王松 , 陈吉龙 , 池晓娟 . 长链非编码RNA编码微肽的鉴定和功能研究进展 . | 福建农林大学学报(自然科学版) , 2025 , 54 (01) , 89-99 . |
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Vaccine adjuvants are now widely utilized in vaccine formulations. The IFN-Stimulated Genes (ISGs) family, play crucial roles in immune regulation and exhibit broad-spectrum antiviral activity. However, limited studies have investigated the potential of ISGs as vaccine adjuvants. Here, three swine ISGs fusion proteins were induced and purified from Escherichia coli, including IFITM1, IFITM3 and Viperin (sIFITM1, sIFITM3, and sViperin). Furthermore, sIFITM1, sIFITM3, and sViperin inhibited the replication of pseudorabies virus (PRV) in swine (PK-15 and 3D4/21) and murine (NIH/3 T3 and C57/B6-L) cells. Importantly, these fusion proteins effectively enhanced the immunogenicity of inactivated classical swine fever virus (CSFV) vaccine and improved the immune response in vaccinated mice. Our evidence indicates that, compared with the CSFV vaccine group, the co-administration of sIFITM1, sIFITM3, and sViperin with CSFV vaccine significantly improved humoral immunity, increased T lymphocyte proliferation in the spleen, and elevated serum IgG antibody levels. In conclusion, this study successfully prepared sIFITM1, sIFITM3, and sViperin fusion proteins, confirming their ability to inhibit PRV replication and suggesting their potential as vaccine adjuvants.
Keyword ：
antiviral proteins antiviral proteins IFN-Stimulated Genes (ISGs) IFN-Stimulated Genes (ISGs) inactivated CSFV vaccine inactivated CSFV vaccine protection efficiency protection efficiency vaccine adjuvants vaccine adjuvants
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| GB/T 7714 | Liu, Shasha , Zhou, Wenzhuo , Ye, Haobo et al. sIFITM1, sIFITM3, and sViperin antiviral proteins as inactivated CSFV vaccine adjuvants [J]. | FRONTIERS IN VETERINARY SCIENCE , 2025 , 12 . |
| MLA | Liu, Shasha et al. "sIFITM1, sIFITM3, and sViperin antiviral proteins as inactivated CSFV vaccine adjuvants" . | FRONTIERS IN VETERINARY SCIENCE 12 (2025) . |
| APA | Liu, Shasha , Zhou, Wenzhuo , Ye, Haobo , Qiu, Feng , Gu, Rongrong , Xu, Erying et al. sIFITM1, sIFITM3, and sViperin antiviral proteins as inactivated CSFV vaccine adjuvants . | FRONTIERS IN VETERINARY SCIENCE , 2025 , 12 . |
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本发明提出了将猪源抗病毒蛋白IFITM1、IFITM3、Viperin作为免疫增强剂，应用于猪瘟病毒E2蛋白抗病毒杆状病毒灭活疫苗。当猪源抗病毒蛋白IFITM1、IFITM3、Viperin作为免疫增强剂，与猪瘟病毒E2蛋白抗病毒杆状病毒灭活疫苗一同用于免疫时，能促使免疫小鼠产生更高含量的猪瘟病毒血清IgG抗体。就目前国内市场而言，尚未有猪用抗病毒蛋白免疫增强剂灭活疫苗投入使用。而本方案所采用的猪用免疫增强剂搭配猪瘟病毒E2蛋白抗病毒杆状病毒灭活疫苗，不仅可更为有效地防控猪瘟，还填补了当下养猪市场在免疫增强剂方面的空白。
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| GB/T 7714 | 陈吉龙 , 刘莎莎 . 一种猪源抗病毒蛋白作为免疫增强剂在猪瘟病毒灭活疫苗中的应用 : CN202510543558.2[P]. | 2025-04-28 . |
| MLA | 陈吉龙 et al. "一种猪源抗病毒蛋白作为免疫增强剂在猪瘟病毒灭活疫苗中的应用" : CN202510543558.2. | 2025-04-28 . |
| APA | 陈吉龙 , 刘莎莎 . 一种猪源抗病毒蛋白作为免疫增强剂在猪瘟病毒灭活疫苗中的应用 : CN202510543558.2. | 2025-04-28 . |
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本发明涉及生物医药领域，具体涉及一种由长链非编码RNA MALAT1编码的微肽及其应用，该微肽具体为人源微肽，命名为miPEP5‑P52，在开发新型疫苗作为复制增强剂中的应用。试验证明，人源MALAT1基因所编码的微肽miPEP5‑P52能够促进流感病毒的复制。而且，在小鼠体内高表达miPEP5‑P52，流感病毒刺激后增强病毒载量，促进小鼠死亡。本发明揭示了人源MALAT1基因编码的微肽miPEP5‑P52在细胞和动物水平上均能显著增强流感病毒复制，可作为流感病毒疫苗的复制增强剂，为流感病毒疫苗的研制提供新的选择。
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| GB/T 7714 | 陈吉龙 . 一种由长链非编码RNA MALAT1编码的微肽及其应用 : CN202510023055.2[P]. | 2025-01-07 . |
| MLA | 陈吉龙 . "一种由长链非编码RNA MALAT1编码的微肽及其应用" : CN202510023055.2. | 2025-01-07 . |
| APA | 陈吉龙 . 一种由长链非编码RNA MALAT1编码的微肽及其应用 : CN202510023055.2. | 2025-01-07 . |
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Influenza A virus (IAV) poses a significant global health threat to both humans and animals. Increasing evidence highlights long noncoding RNAs (lncRNAs) as critical regulators of various physiological and pathological processes, but their roles in virus-host interactions in chickens remain elusive. This study identified chicken lncRNAs and further investigated their functional involvement in IAV-host interactions. Transcriptome sequencing of chicken DF-1 cells revealed that 2118 lncRNAs were differentially expressed following H9N2 avian influenza virus infection. Among these, a lncRNA that we named lncRNA-up4 was significantly upregulated by H9N2 infection. LncRNA-up4 was predominantly localized in the nucleus but also detected in the cytoplasm. Furthermore, we found that the virus-induced expression of lncRNA-up4 was regulated by the pattern recognition receptor-dependent NF-kappa B signalling pathway. Functional analysis demonstrated that silencing lncRNA-up4 impaired IAV replication by upregulating the expression of several critical antiviral molecules, including IFN-beta, MX1, and OAS-1. Conversely, overexpression of lncRNA-up4 increased viral replication, as evidenced by increased viral NP protein and virus titers. Moreover, we observed that lncRNA-up4 positively regulated the expression of IL-6, TNF-alpha, and IL-1 beta but suppressed the expression of IFN-beta at the mRNA level. These results reveal that the newly identified lncRNA-up4 plays an important role in influenza virus replication through the regulation of cytokine production and antiviral gene expression in chicken cells. This study provides valuable insight into the regulatory function of chicken lncRNAs in innate immunity.
Keyword ：
Chicken Chicken IL-6 IL-6 interferon interferon LncRNA LncRNA NF-kappa B NF-kappa B
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| GB/T 7714 | Sajjad, Nelam , Maarouf, Mohamed , Wang, Yiming et al. A chicken lncRNA is identified as a critical regulator that increases influenza virus replication by impairing innate antiviral responses [J]. | VETERINARY RESEARCH , 2025 , 56 (1) . |
| MLA | Sajjad, Nelam et al. "A chicken lncRNA is identified as a critical regulator that increases influenza virus replication by impairing innate antiviral responses" . | VETERINARY RESEARCH 56 . 1 (2025) . |
| APA | Sajjad, Nelam , Maarouf, Mohamed , Wang, Yiming , Shrestha, Prasha , Rai, Kul Raj , Chi, Xiaojuan et al. A chicken lncRNA is identified as a critical regulator that increases influenza virus replication by impairing innate antiviral responses . | VETERINARY RESEARCH , 2025 , 56 (1) . |
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Circular RNAs (circRNAs) are a class of non-coding RNAs with a covalently closed circular structure, lacking 5'-caps or 3'-poly(A) tails. They are relatively conserved, highly stable, and often exhibit tissue- or cell-specific production in eukaryotic cells. Based on the advances in sequencing technologies and bioinformatics, multiple reports have suggested that viruses and other microorganisms may encode circRNA-like molecules, providing new insights into the physiological and pathological roles of circRNAs. The innate immune system functions as the body's primary defense mechanism against viral infections. It detects pathogen-associated molecular patterns (PAMPs) and activates signaling pathways to suppress viral replication and limit their spread. CircRNAs are involved in regulation of the host innate immune signaling pathways and play essential roles in viral pathogenesis. It has been shown that circRNAs can regulate gene expression by acting as miRNA sponges or protein sponges, or encoding small proteins in specific cases. For example, previous studies have revealed that circRNAs participate in the host antiviral immune response through the competitive endogenous RNA (ceRNA) network by acting as miRNA sponges. This review highlights research progress in the regulation and functions of host- and virus-encoded circRNAs in host-virus interactions, as well as their potential as diagnostic biomarkers and therapeutic targets in clinical applications.
Keyword ：
CircRNAs CircRNAs host-virus interaction host-virus interaction influenza influenza oncogenic virus oncogenic virus virus infection virus infection
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| GB/T 7714 | Liu, Jiayin , Wang, Yiming , Zheng, Meichun et al. Roles of circRNAs in viral pathogenesis [J]. | FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY , 2025 , 15 . |
| MLA | Liu, Jiayin et al. "Roles of circRNAs in viral pathogenesis" . | FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY 15 (2025) . |
| APA | Liu, Jiayin , Wang, Yiming , Zheng, Meichun , Du, Jiayuan , Maarouf, Mohamed , Chen, Ji-Long . Roles of circRNAs in viral pathogenesis . | FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY , 2025 , 15 . |
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The H12 subtypes of avian influenza viruses (AIVs) are globally prevalent in wild birds, occasionally spilling over into poultry. In this study, we isolated an H12N8 virus from ducks in a live poultry market. Full genomic analysis revealed that the virus bears a single basic amino acid in the cleavage site of the hemagglutinin gene. Phylogenetic analysis revealed that the eight gene segments of the H12N8 virus belong to the Eurasian lineage and the HA gene was clustered with wild bird-originated H12 viruses, with its NP gene showing the highest nucleotide similarity to 2013-like H7N9 viruses. The H12N8 virus replicated effectively in both mammalian and avian cells without prior adaptation. Moreover, the H12N8 virus could infect and replicate in the upper respiratory tract of BALB/c mice without prior adaptation. The H12N8 virus replicated and transmitted inefficiently in both ducks and chickens and hardly triggered high hemagglutination inhibition (HI) antibody titers in the inoculated and contact animals. These results suggest that the wild bird-origin H12N8 virus has reassorted with viruses circulating in domestic poultry, but it inefficiently replicates and transmits in avian hosts. Our findings demonstrate that H12N8 AIV has emerged in domestic poultry, emphasizing the importance of active surveillance of AIVs in both wild and domestic birds.
Keyword ：
avian influenza virus avian influenza virus evolution evolution pathogenicity pathogenicity transmissibility transmissibility
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| GB/T 7714 | Zhao, Conghui , Huang, Jiacheng , Zhang, Chunping et al. Characteristics of the First Domestic Duck-Origin H12N8 Avian Influenza Virus in China [J]. | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES , 2025 , 26 (6) . |
| MLA | Zhao, Conghui et al. "Characteristics of the First Domestic Duck-Origin H12N8 Avian Influenza Virus in China" . | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 26 . 6 (2025) . |
| APA | Zhao, Conghui , Huang, Jiacheng , Zhang, Chunping , Wang, Yang , Zhang, Xiaoxuan , Liu, Sha et al. Characteristics of the First Domestic Duck-Origin H12N8 Avian Influenza Virus in China . | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES , 2025 , 26 (6) . |
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Micropeptides (miPEPs), encoded by short open reading frames (sORFs) within various genomic regions, have recently emerged as critical regulators of multiple biological processes. In particular, these small molecules are now increasingly being recognized for their role in modulating viral replication, pathogenesis, and host immune responses. Both host miPEPs and virus-derived miPEPs have been noted for their ability to regulate virus-host interactions through diversified mechanisms such as altering protein stability and modulating protein-protein interactions. Although thousands of sORFs have been annotated as having the potential to encode miPEPs, only a small number have been experimentally validated so far, with some directly linked to virus-host interactions and a small subset associated with immune modulation, indicating that the investigation of miPEPs is still in its infancy. The systematic identification, translational status assessment, in-depth characterization, and functional analysis of a substantial fraction of sORFs encoding miPEPs remain largely underexplored. Further studies are anticipated to uncover the intricate mechanisms underlying virus-host interactions, host immune modulation, and the broader biological functions of miPEPs. This article will review the emerging roles of miPEPs in virus-host interactions and host immunity, and discuss the challenges and future perspectives of miPEP studies.
Keyword ：
innate immunity innate immunity micropeptides micropeptides sORFs sORFs virus-host interactions virus-host interactions
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| GB/T 7714 | Sun, Haowen , Gu, Rongrong , Tang, Tingting et al. Current Perspectives on Functional Involvement of Micropeptides in Virus-Host Interactions [J]. | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES , 2025 , 26 (8) . |
| MLA | Sun, Haowen et al. "Current Perspectives on Functional Involvement of Micropeptides in Virus-Host Interactions" . | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 26 . 8 (2025) . |
| APA | Sun, Haowen , Gu, Rongrong , Tang, Tingting , Rai, Kul Raj , Chen, Ji-Long . Current Perspectives on Functional Involvement of Micropeptides in Virus-Host Interactions . | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES , 2025 , 26 (8) . |
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Vaccination is one of the most effective methods for controlling animal infectious diseases, and the use of adjuvants plays crucial role in enhancing the immune efficacy of vaccines, particularly in inactivated and subunit vaccines. With the continuous advancement of research in animal immunology and immune mechanisms, our understanding of the functions of cells and cytokines in immune responses has become increasingly comprehensive, laying a solid foundation for the development of novel vaccines and adjuvants. Cytokines are a class of proteins secreted by the animal body that regulate innate and adaptive immune responses through interaction with specific receptors. To date, numerous studies have investigated the potential of using cytokines as adjuvants to enhance the efficacy of veterinary vaccines. This review focuses on cytokines as veterinary vaccine adjuvants, with special attention to the current research progress and mechanisms of cytokines such as interleukins, interferons, chemokines, and colony-stimulating factors. Additionally, examples of the application of cytokine-based adjuvants in combination with veterinary vaccines will be discussed to provide further insights and references for the development of cytokine-based veterinary adjuvants.
Keyword ：
cytokines cytokines immune enhancement immune enhancement vaccination vaccination vaccine adjuvant vaccine adjuvant veterinary vaccine veterinary vaccine
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| GB/T 7714 | Yan, Shihong , Chen, Huimin , Liu, Qiao et al. Harnessing cytokines: innovative adjuvants for improved veterinary vaccine efficacy [J]. | FRONTIERS IN IMMUNOLOGY , 2025 , 16 . |
| MLA | Yan, Shihong et al. "Harnessing cytokines: innovative adjuvants for improved veterinary vaccine efficacy" . | FRONTIERS IN IMMUNOLOGY 16 (2025) . |
| APA | Yan, Shihong , Chen, Huimin , Liu, Qiao , Zhang, Xinyu , Wei, Qiulu , Hu, Chenqi et al. Harnessing cytokines: innovative adjuvants for improved veterinary vaccine efficacy . | FRONTIERS IN IMMUNOLOGY , 2025 , 16 . |
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