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学者姓名：林瑞意
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Context The occurrence of apoptosis due to transient oxidative damage to spermatozoa presents a significant challenge in semen preservation. At present, the addition of protective agents is the primary method for mitigating this damage and involves the addition of protective agents.Aims This study aims to elucidate the mechanism of action of cryopreservation and to identify high-quality combinatorial cryopreservation dilutions.Methods In this study, two protective agents, bovine serum albumin (BSA) and sodium alginate (SA), were combined to evaluate the effectiveness of these novel additives in protecting porcine sperm from damage during cryopreservation. The mechanism of action of the SA and BSA combination was further elucidated at the molecular level, with key proteins being identified through proteomic analysis.Key results The findings indicated that sperm treated with 2 mg/mL SA and 5 mg/mL BSA exhibited optimal motility parameters, superior functional integrity and the most effective ability to alleviate oxidative stress. Combined with proteomic data, the results suggested that these additives regulate improvements in sperm quality by mediating the activity of the PI3K-AKT pathway.Conclusions This study found that the combination of SA and BSA provides an effective protective effect for frozen sperm preservation.Implications The findings offer theoretical and technical support for the use of composite additive to protect sperm from damage, which is crucial for enhancing the quality of pig semen and improving reproductive outcomes.
Keyword ：
bovine serum albumin bovine serum albumin oxidative stress oxidative stress PI3K-AKT pathway PI3K-AKT pathway pig pig proteomics proteomics sodium alginate sodium alginate sperm cryopreservation sperm cryopreservation sperm motility sperm motility
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| GB/T 7714 | Wang, Jing , Rao, Yongyong , Du, Chongfan et al. Sodium alginate and bovine serum albumin co-combined improved the cryopreservation quality of boar sperm through the PI3K-AKT pathway [J]. | REPRODUCTION FERTILITY AND DEVELOPMENT , 2025 , 37 (5) . |
| MLA | Wang, Jing et al. "Sodium alginate and bovine serum albumin co-combined improved the cryopreservation quality of boar sperm through the PI3K-AKT pathway" . | REPRODUCTION FERTILITY AND DEVELOPMENT 37 . 5 (2025) . |
| APA | Wang, Jing , Rao, Yongyong , Du, Chongfan , Wang, Ao , Wu, Yanling , Lin, Ruiyi et al. Sodium alginate and bovine serum albumin co-combined improved the cryopreservation quality of boar sperm through the PI3K-AKT pathway . | REPRODUCTION FERTILITY AND DEVELOPMENT , 2025 , 37 (5) . |
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Improving egg quality and enhancing production efficiency are essential goals in poultry breeding. CDH5 encodes a cadherin involved in Ca2+ transport in endothelial cells. The role of CDH5 in regulating duck egg quality and its mechanisms affecting Ca2+ concentrations in duck uterine epithelial cells remains unclear. This study evaluated egg quality traits of the Putian black duck and conducted an association analysis with blood biochemical indices and single nucleotide polymorphisms in CDH5. Additionally, we constructed a CDH5 overexpression vector and synthesized specific siRNAs for transfection into Putian black duck uterine epithelial cells to assess Ca2+ concentrations. Our results revealed a significant association between egg quality and five novel SNPs in CDH5, along with various blood biochemical indices. Further experiments demonstrated that CDH5 overexpression and knockdown reduced Ca2+ concentrations in the uterine epithelial cells of Putian black ducks.
Keyword ：
Ca2+ concentration Ca2+ concentration CDH5 CDH5 Duck uterine epithelial cells Duck uterine epithelial cells Egg quality Egg quality Serum biochemistry Serum biochemistry
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| GB/T 7714 | Li, Huihuang , Yang, Yue , Yang, Fan et al. Determination of blood biochemical indices and research of egg quality-related candidate gene CDH5 in Putian black duck [J]. | GENE , 2025 , 937 . |
| MLA | Li, Huihuang et al. "Determination of blood biochemical indices and research of egg quality-related candidate gene CDH5 in Putian black duck" . | GENE 937 (2025) . |
| APA | Li, Huihuang , Yang, Yue , Yang, Fan , Bao, Xinguo , Pan, Chengfu , Lin, Weilong et al. Determination of blood biochemical indices and research of egg quality-related candidate gene CDH5 in Putian black duck . | GENE , 2025 , 937 . |
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Egg weight is a primary economic trait in poultry breeding. Putian Black duck, an excellent local laying duck breed in Fujian Province, includes two different strains, black feather strain and white feather strain. The white feather strain of Putian Black duck is also known as Putian White duck. Except for the different feather colors, these two strains differ in egg weight. In this study, whole-genome resequencing was conducted on Putian Black duck and Putian White duck to explore the differences in the genetic mechanism of egg weight. LRP8, VLDLR, and LPL were identified as key candidate genes affecting egg weight. Mass spectrometry was used to detect the SNPs of LRP8, VLDLR, and LPL. Result indicates that the SNPs of LRP8, VLDLR, and LPL in both populations exhibited moderate polymorphism, and Putian Black duck possessed higher genetic variation and potential selectivity. Association analysis indicated that in Putian Black duck, four SNPs in the LRP8 gene were significantly associated with egg weight. These loci can be used as molecular markers for improving egg weight in Putian Black duck.
Keyword ：
egg weight egg weight Putian black duck Putian black duck resequencing resequencing SNP SNP
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| GB/T 7714 | Yang, Yinhua , Lin, Weilong , Li, Huihuang et al. Identification of candidate genes affecting egg weight trait of Putian Black duck based on whole genome resequencing [J]. | ANIMAL BIOTECHNOLOGY , 2025 , 36 (1) . |
| MLA | Yang, Yinhua et al. "Identification of candidate genes affecting egg weight trait of Putian Black duck based on whole genome resequencing" . | ANIMAL BIOTECHNOLOGY 36 . 1 (2025) . |
| APA | Yang, Yinhua , Lin, Weilong , Li, Huihuang , Yang, Fan , Bao, Xinguo , Pan, Chengfu et al. Identification of candidate genes affecting egg weight trait of Putian Black duck based on whole genome resequencing . | ANIMAL BIOTECHNOLOGY , 2025 , 36 (1) . |
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Intramuscular fat (IMF) deposition is a critical determinant of pork quality, with pre-adipocyte differentiation serving as a pivotal regulatory process. Although muscle-derived interleukin-6 (IL-6) is recognized for its role in promoting lipolysis and fatty acid oxidation, the epigenetic mechanisms governing its regulation remain poorly characterized. This study elucidates the epigenetic regulation of histone deacetylase 5 (HDAC5) on muscle-derived IL-6 transcription and secretion, and its consequent effects on porcine IMF deposition. Western blot (WB) and quantitative PCR (qPCR) analyses demonstrated that elevated IL-6 expression in the porcine longissimus dorsi muscle was negatively correlated with IMF content. Chromatin immunoprecipitation (ChIP)-PCR and adipogenic induction assays revealed that HDAC5 suppresses IL-6 transcription and secretion, thereby enhancing intramuscular pre-adipocyte differentiation. Through co-immunoprecipitation (Co-IP), dual-luciferase reporter assays, and differentiation induction experiments, we identified a functional interaction between HDAC5 and myocyte enhancer factor 2A (MEF2A). Mechanistically, MEF2A binds to the IL-6 promoter to activate its transcription and secretion, which subsequently inhibits pre-adipocyte differentiation. Dual-interference assays further confirmed that HDAC5 regulates IL-6 expression by directly binding to and repressing MEF2A activity, ultimately modulating IMF deposition. These findings uncover a novel epigenetic pathway through which HDAC5 governs muscle-derived IL-6 to regulate pre-adipocyte differentiation and IMF accumulation in pigs, offering critical molecular insights for strategies aimed at optimizing pork quality.
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| GB/T 7714 | Weimin Lin , Hongbing Song , Chongfan Du et al. HDAC5 mediates the regulation of muscle-derived interleukin 6 on the differentiation of porcine intramuscular pre-adipocytes. [J]. | Domestic animal endocrinology , 2025 , 95 . |
| MLA | Weimin Lin et al. "HDAC5 mediates the regulation of muscle-derived interleukin 6 on the differentiation of porcine intramuscular pre-adipocytes." . | Domestic animal endocrinology 95 (2025) . |
| APA | Weimin Lin , Hongbing Song , Chongfan Du , Jing Wang , Xiaohan Zhang , Chu Yang et al. HDAC5 mediates the regulation of muscle-derived interleukin 6 on the differentiation of porcine intramuscular pre-adipocytes. . | Domestic animal endocrinology , 2025 , 95 . |
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The coloration of plumage in poultry species has substantial economic significance. Putian black ducks encompass 2 distinct strains characterized by black and white plumage variations resulting from selective breeding. This study aimed to identify the molecular mechanisms responsible for plumage coloration in these 2 distinct strains. A comprehensive genome-wide association study was conducted using DNA data sourced from a F2 segregating population, consisting of 71 individuals with black plumage and 39 individuals with white plumage, derived from these distinct 2 strains. This analysis revealed that 894 nucleo-tide polymorphisms and identified 58 candidate genes. Subsequent Gene Ontology and Kyoto Encyclopedia of Genes and Genomes coenrichment analyses identified MITF as a key candidate gene implicated in melanin biosynthesis. Furthermore, extensive screening of significant polymorphic loci within MITF was carried out via mass spectrometry in 3 distinct populations: 100 individuals with black plumage and 100 individuals with white plumage from the F0 generation; and 50 with black plumage form the F1 generation). Eighteen candidate polymorphic loci were identified demonstrating significant associations with variations in black and white plumage. Notably, 8 of these loci were located within the 2,000 bp region upstream of MITF-M. To validate the critical regulatory role of MITF-M in black and white plumage formation, a dual-fluorescence reporter system was constructed, and dual-fluorescence activity was assessed. The results revealed that the fluorescence activity at wild-type sites (corresponding to black plum-age) was significantly higher than that at the mutant -type sites (associated with white plumage) (P < 0.01). To corroborate the pivotal role of MITF-M in black and white plumage formation, qPCR was employed to evalu-ate the expression levels of various MITF variants in black and white feather bulbs. This analysis demonstrated that only MITF-M exhibited specific expression in black feather bulbs. These results elucidate the central role of polymorphic mutations within the MITF promoter region in the regulation of black and white plumage coloration in Putian black ducks. This study extends our understanding of mechanisms governing duck plumage coloration and provides valuable molecu-lar markers for future research in duck production and breeding based on plumage coloration.
Keyword ：
duck duck melanin melanin MITF-M MITF-M plumage color plumage color SNPs SNPs
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| GB/T 7714 | Lin, Ruiyi , Zhao, Fanglu , Xiong, Taimin et al. Genetic mapping identifies SNP mutations in MITF-M promoter associated with melanin formation in Putian black duck [J]. | POULTRY SCIENCE , 2024 , 103 (1) . |
| MLA | Lin, Ruiyi et al. "Genetic mapping identifies SNP mutations in MITF-M promoter associated with melanin formation in Putian black duck" . | POULTRY SCIENCE 103 . 1 (2024) . |
| APA | Lin, Ruiyi , Zhao, Fanglu , Xiong, Taimin , Lai, Lianjie , Li, Huihuang , Lin, Weilong et al. Genetic mapping identifies SNP mutations in MITF-M promoter associated with melanin formation in Putian black duck . | POULTRY SCIENCE , 2024 , 103 (1) . |
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The antioxidant properties of crocin are attracting interest, yet the underlying mechanisms by which crocin mitigates oxidative stress-induced intestinal damage have not been determined. This study aimed to elucidate the effects of crocin on oxidative stress, apoptosis, and intestinal epithelial injury in intestinal epithelial cells (IPEC-J2). Using an H2O2-induced oxidative stress model in IPEC-J2 cells, crocin was added to assess its effects. Cell viability and apoptosis were evaluated using methyl thiazolyl tetrazolium assays and flow cytometry. Additionally, oxidative stress markers, such as superoxide dismutase (SOD), catalase (CAT), reactive oxygen species (ROS), and malondialdehyde (MDA), were quantified. We investigated, in which cell oxidation and apoptosis were measured at the gene and protein levels and employed transcriptome analysis to probe the mechanism of action and validate relevant pathways. The results showed that crocin ameliorates H2O2-induced oxidative stress by reducing ROS and MDA levels and by countering the reductions in CAT, total antioxidant capacity, and SOD. Crocin also attenuates the upregulation of key targets in the Nrf2 pathway. Furthermore, it effectively mitigated IPEC-J2 cell apoptosis caused by oxidative stress, as evidenced by changes in cell cycle factor expression, apoptosis rate, mitochondrial membrane potential, and apoptosis pathway activity. In addition, crocin preserves the integrity of the intestinal barrier by protecting tight junction proteins against oxidative stress. Transcriptome sequencing analysis suggested that the mitochondrial pathway may be a crucial mechanism through which crocin exerts its protective effects. In summary, crocin decreases oxidative stress molecule formation, inhibits Nrf2 pathway activity, prevents apoptosis-induced damage, enhances oxidative stress resistance in IPEC-J2 cells, and maintains redox balance in the pig intestine.
Keyword ：
apoptosis apoptosis crocin crocin IPEC-J2 IPEC-J2 oxidative stress oxidative stress pig pig
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| GB/T 7714 | Wang, Jing , Song, Hongbing , Huang, Yongjie et al. Protective effect of crocin on peroxidation-induced oxidative stress and apoptosis in IPEC-J2 cells [J]. | ENVIRONMENTAL TOXICOLOGY , 2024 . |
| MLA | Wang, Jing et al. "Protective effect of crocin on peroxidation-induced oxidative stress and apoptosis in IPEC-J2 cells" . | ENVIRONMENTAL TOXICOLOGY (2024) . |
| APA | Wang, Jing , Song, Hongbing , Huang, Yongjie , Yang, Chu , Wu, Yanling , Lin, Ruiyi et al. Protective effect of crocin on peroxidation-induced oxidative stress and apoptosis in IPEC-J2 cells . | ENVIRONMENTAL TOXICOLOGY , 2024 . |
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Fujian Muscovy duck is a well-known meat waterfowl in Fujian Province due to its high meat production, superior breeding potential, and strong resistance. To fully explore the genetic characteristics of these advantages, Fujian black Muscovy duck and white Muscovy duck were used for whole-genome re-sequencing and transcriptome analyses. Population structure analysis showed significant differentiation between the two feather strains. Runs of homozygosity analysis indicated a stronger artificial influence on the black-feathered strain, with ROH island genes notably enriched in muscle tissue-related terms and pathways. Selective sweep and transcriptome analysis revealed a significant enrichment of genes linked to muscle tissue and muscle fiber-related terms and pathways. Key candidate genes identified, such as MEF2C, MYOZ2, and METTL21C, are believed to play crucial roles in meat production in Fujian Muscovy duck. This study offers a new perspective on improving meat production in Fujian Muscovy duck, which can benefit breeding strategies and production management.
Keyword ：
Fujian Muscovy duck Fujian Muscovy duck Muscle growth Muscle growth Transcriptome analysis Transcriptome analysis Whole genome re-sequencing Whole genome re-sequencing
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| GB/T 7714 | Lin, Ruiyi , Li, Huihuang , Lai, Lianjie et al. Analysis of genetic structure and identification of important genes associated with muscle growth in Fujian Muscovy duck [J]. | POULTRY SCIENCE , 2024 , 103 (12) . |
| MLA | Lin, Ruiyi et al. "Analysis of genetic structure and identification of important genes associated with muscle growth in Fujian Muscovy duck" . | POULTRY SCIENCE 103 . 12 (2024) . |
| APA | Lin, Ruiyi , Li, Huihuang , Lai, Lianjie , Yang, Fan , Qiu, Jialing , Lin, Weilong et al. Analysis of genetic structure and identification of important genes associated with muscle growth in Fujian Muscovy duck . | POULTRY SCIENCE , 2024 , 103 (12) . |
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BackgroundJianli pig, a renowned indigenous breed in China, has the characteristics of a two-end black (TEB) coat color, excellent meat quality, strong adaptability and increased prolificacy. However, there is limited information available regarding the genetic diversity, population structure and genomic regions under selection of Jianli pig. On the other hand, the genetic mechanism of TEB coat color has remained largely unknown.ResultsIn this study, the whole genome resequencing of 30 Jianli pigs within a context of 153 individuals representing 13 diverse breeds was performed. The population structure analysis revealed that Jianli pigs have close genetic relationships with the Tongcheng pig breed, their geographical neighbors. Three methods (observed heterozygosity, expected heterozygosity, and runs of homozygosity) implied a relatively high level of genetic diversity and, a low inbreeding coefficient in Jianli compared with other pigs. We used Fst and XP-EHH to detect the selection signatures in Jianli pigs compared with Asian wild boar. A total of 451 candidate genes influencing meat quality (CREBBP, ADCY9, EEPD1 and HDAC9), reproduction (ESR1 and FANCA), and coat color (EDNRB, MITF and MC1R), were detected by gene annotation analysis. Finally, to fine-map the genomic region for the two-end black (TEB) coat color phenotype in Jianli pigs, we performed three signature selection methods between the TEB coat color and no-TEB coat color pig breeds. The current study, further confirmed that the EDNRB gene is a candidate gene for TEB color phenotype found in Chinese pigs, including Jinhua pigs, and the haplotype harboring 25 SNPs in the EDNRB gene may promote the formation of TEB coat color. Further ATAC-seq and luciferase reporter assays of these regions suggest that the 25-SNPs region was a strong candidate causative mutation that regulates the TEB coat color phenotype by altering enhancer function.ConclusionOur results advanced the understanding of the genetic mechanism behind artificial selection, and provided further resources for the protection and breeding improvement of Jianli pigs.
Keyword ：
Coat color Coat color Genetic diversity Genetic diversity Jianli pig Jianli pig Selection signatures Selection signatures Whole-genome sequencing Whole-genome sequencing
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| GB/T 7714 | Xu, Zhong , Wu, Junjing , Zhang, Yu et al. Genome-wide detection of selection signatures in Jianli pigs reveals novel cis-regulatory haplotype in EDNRB associated with two-end black coat color [J]. | BMC GENOMICS , 2024 , 25 (1) . |
| MLA | Xu, Zhong et al. "Genome-wide detection of selection signatures in Jianli pigs reveals novel cis-regulatory haplotype in EDNRB associated with two-end black coat color" . | BMC GENOMICS 25 . 1 (2024) . |
| APA | Xu, Zhong , Wu, Junjing , Zhang, Yu , Qiao, Mu , Zhou, Jiawei , Feng, Yue et al. Genome-wide detection of selection signatures in Jianli pigs reveals novel cis-regulatory haplotype in EDNRB associated with two-end black coat color . | BMC GENOMICS , 2024 , 25 (1) . |
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The assessment of animal genetic structure had significant importance for the preservation and breeding of animal germplasm resources. Selection signals are genotype markers generated during the process of biological evolution, and the detection of selection signals could reveal the direction of species evolution. The aim of this study was to generate a whole-genome resequencing data from Jinding duck, Shanma duck, Youxian Partridge duck, and Taiwan Brown tsaiya duck to reveal their population structure and selection signals. The population structure analysis revealed significant genetic differences among the 4 indigenous laying ducks, indicating their independent lineage. Specifically, Shanma duck and Youxian partridge duck were closely and likely originated from a common ancestor. In addition, selection sweep analysis was performed using the population genetic differentiation coefficient (Fst) and nucleotide diversity ratio (p ratio). The top 5% was used as the threshold for the Fst and p ratio, and the 2 thresholds were combined to identify selected genomic regions. In the selected regions of the 3 comparison groups, 136, 143, and 268 candidate genes were detected. Further screening of all candidate genes revealed that 35 candidate genes appeared simultaneously in 3 comparative groups, with 16 genes annotated. The 16 genes were analyzed by Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses. The results revealed 5 functional genes (AQP3, PIK3C3, NOL6, RPP25, and DCTN3) that may be related to important economic traits in laying ducks and involved mainly invasopressin-regulated water reabsorption, ribosome biogenesis, and the PI3K signaling pathway. The results provide insights into the protection and exploitation of genetic resources of Chinese indigenous laying ducks.
Keyword ：
laying duck laying duck population structure population structure resequencing resequencing selection signal selection signal
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| GB/T 7714 | Zhu, Zhiming , Lin, Ruiyi , Zhao, Bangzhe et al. Whole-genome resequencing revealed the population structure and selection signal of 4 indigenous Chinese laying ducks [J]. | POULTRY SCIENCE , 2024 , 103 (7) . |
| MLA | Zhu, Zhiming et al. "Whole-genome resequencing revealed the population structure and selection signal of 4 indigenous Chinese laying ducks" . | POULTRY SCIENCE 103 . 7 (2024) . |
| APA | Zhu, Zhiming , Lin, Ruiyi , Zhao, Bangzhe , Shi, Wenli , Cai, Qiannan , Zhang, Linli et al. Whole-genome resequencing revealed the population structure and selection signal of 4 indigenous Chinese laying ducks . | POULTRY SCIENCE , 2024 , 103 (7) . |
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Owing to the unique geography and the isolated environment, Guanling cattle, which is one of five local cattle breeds in Guizhou, China, has developed unique characteristics. The number of pure Guanling cattle decreased markedly because of the hybridization with foreign breeds. In the present study, the maternal genetic diversity of 58 Guanling bulls was assessed by whole mitochondrial genome sequencing. Genetic polymorphisms and phylogenetic analyses classified Guanling cattle into two main lineages, where 43.10% of Guanling cattle were closely related to the foreign breeds and 56.90% displayed distinct features in mitochondrial genomic diversity. PCA analysis further separated Guanling cattle into four populations, one of which was clustered with the foreign breeds. The result of the structure plot and genetic polymorphisms revealed high genetic diversity within two populations that have a long genetic distance from the foreign breeds. Overall, our findings suggest that the whole mitochondrial genome sequencing analysis is a useful and reliable tool to study maternal genetic diversity and to identify the pure population of Guanling cattle. The results will be beneficial to the breeding management of Guanling cattle.
Keyword ：
Breeding management Breeding management Genetic diversity Genetic diversity Guanling cattle Guanling cattle Mitochondrial genome Mitochondrial genome
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| GB/T 7714 | Xu, Longxin , Wang, Xin , Wang, Hua et al. Maternal Genetic Diversity Analysis of Guanling Cattle by Mitochondrial Genome Sequencing [J]. | BIOCHEMICAL GENETICS , 2024 , 63 (6) : 5003-5012 . |
| MLA | Xu, Longxin et al. "Maternal Genetic Diversity Analysis of Guanling Cattle by Mitochondrial Genome Sequencing" . | BIOCHEMICAL GENETICS 63 . 6 (2024) : 5003-5012 . |
| APA | Xu, Longxin , Wang, Xin , Wang, Hua , Wu, Junda , Zhou, Wenzhang , Ni, Mengmeng et al. Maternal Genetic Diversity Analysis of Guanling Cattle by Mitochondrial Genome Sequencing . | BIOCHEMICAL GENETICS , 2024 , 63 (6) , 5003-5012 . |
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