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学者姓名：陈大福
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Abstract ：
Ascosphaera apis is a fungal pathogen that specifically infects bee larvae, causing an outbreak of chalkbrood disease in the bee colony and a decline in the number of bee colonies. The role of miRNA regulation in honeybees in response to A. apis infection is unclear. In this study, based on small RNA-seq, we identified the differentially expressed miRNAs (DEmiRNAs) and their regulatory networks and functions in the gut of Apis cerana cerana on the first day (AcT1), the second day (AcT2) and the third day (AcT3) after A. apis infection, and analyzed the immune response mechanism of A. apis through the miRNAs-mRNA regulation network of A. apis infection. A total of 537 miRNAs were obtained, and 10, 27, and 54 DEmiRNAs were screened in the AcT1, AcT2, and AcT3 groups, respectively. The number of DEmiRNAs gradually increased with the infection time. Stem-loop RT-PCR results showed that most of the DEmiRNAs were truly expressed, and the expression trend of DEmiRNAs was consistent with the results of sRNA-seq. The top five GO terms of DEmiRNA-targeted mRNA were binding, cellular process, catalytic activity, metabolic process, and single-organism process. The main pathways enriched by KEGG were endocytosis, ubiquitin-mediated proteolysis, phagosome, and the JAK-STAT immune-related signaling pathways. The number of DEmiRNAs and target mRNAs of these related pathway genes increased with infection time. The miRNA-mRNA regulatory network analysis showed that ace-miR-539-y was the core miRNA of the early immune response in the gut of larvae infected with A. apis in the JAK-STAT pathway and phagosome, and ace-miR-1277-x was the core miRNA of the late immune response in the gut of larvae infected with A. apis in the JAK-STAT signaling pathway and phagosome. The results showed that miRNA participated in the immune response of honeybees to A. apis infection by regulating the host's energy metabolism, cellular immunity, and humoral immunity. The results of this study provide a basis for the regulation of miRNAs in A. c. cerana larvae in response to A. apis infection and provide new insights into host-pathogen interactions.
Keyword ：
Apis cerana Apis cerana Ascosphaera apis Ascosphaera apis immune response immune response larvae larvae miRNA miRNA transcriptome transcriptome
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| GB/T 7714 | Song, Yuxuan , Qiu, Jianfeng , Kang, Jing et al. Transcriptomic Characterization of miRNAs in Apis cerana Larvae Responding to Ascosphaera apis Infection [J]. | GENES , 2025 , 16 (2) . |
| MLA | Song, Yuxuan et al. "Transcriptomic Characterization of miRNAs in Apis cerana Larvae Responding to Ascosphaera apis Infection" . | GENES 16 . 2 (2025) . |
| APA | Song, Yuxuan , Qiu, Jianfeng , Kang, Jing , Chen, Ying , Cao, Ruihua , Wang, Wei et al. Transcriptomic Characterization of miRNAs in Apis cerana Larvae Responding to Ascosphaera apis Infection . | GENES , 2025 , 16 (2) . |
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Synthetic biology and nanotechnology fusion represent a transformative approach promoting fundamental and clinical biomedical science development. In SynBioNanoDesign, biological systems are reimagined as dynamic and programmable materials to yield engineered nanomaterials with emerging and specific functionalities. This review elucidates a comprehensive examination of synthetic biology's pivotal role in advancing engineered nanomaterials for targeted drug delivery systems. It begins with exploring the fundamental synergy between synthetic biology and nanotechnology, then highlights the current landscape of nanomaterials in targeted drug delivery applications. Subsequently, the review discusses the design of novel nanomaterials informed by biological principles, focusing on expounding the synthetic biology tools and the potential for developing advanced nanomaterials. Afterward, the research advances of innovative materials design through synthetic biology were systematically summarized, emphasizing the integration of genetic circuitry to program nanomaterial responses. Furthermore, the challenges, current weaknesses and opportunities, prospective directions, and ethical and societal implications of SynBioNanoDesign in drug delivery are elucidated. Finally, the review summarizes the transformative impact that synthetic biology may have on drug-delivery technologies in the future.
Keyword ：
Engineered nanomaterials Engineered nanomaterials Genetic circuitry Genetic circuitry Nanobiotechnology Nanobiotechnology Personalized medicine Personalized medicine Regulatory challenges Regulatory challenges Synthetic biology Synthetic biology Targeted drug delivery Targeted drug delivery
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| GB/T 7714 | Cai, Qian , Guo, Rui , Chen, Dafu et al. SynBioNanoDesign: pioneering targeted drug delivery with engineered nanomaterials [J]. | JOURNAL OF NANOBIOTECHNOLOGY , 2025 , 23 (1) . |
| MLA | Cai, Qian et al. "SynBioNanoDesign: pioneering targeted drug delivery with engineered nanomaterials" . | JOURNAL OF NANOBIOTECHNOLOGY 23 . 1 (2025) . |
| APA | Cai, Qian , Guo, Rui , Chen, Dafu , Deng, Zixin , Gao, Jiangtao . SynBioNanoDesign: pioneering targeted drug delivery with engineered nanomaterials . | JOURNAL OF NANOBIOTECHNOLOGY , 2025 , 23 (1) . |
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Ascosphaera apis, a specialized fungal pathogen, causes lethal infection in honeybee larvae. miRNA-like small RNAs (milRNAs) are fungal small non-coding RNAs similar to miRNAs, which have been shown to regulate fungal hyphal growth, spore formation, and pathogenesis. Based on the transcriptome data, differentially expressed miRNA-like RNAs (DEmilRNAs) in A. apis infecting the Apis cerana cerana worker 4-, 5-, and 6-day-old larvae (Aa-4, Aa-5, and Aa-6) were screened and subjected to trend analysis, followed by target prediction and annotation as well as investigation of regulatory networks, with a focus on sub-networks relative to MAPK signaling pathway, glycerolipid metabolism, superoxide dismutase, and enzymes related to chitin synthesis and degradation. A total of 606 milRNAs, with a length distribution ranging from 18 nt to 25 nt, were identified. The first nucleotide of these milRNAs presented a bias toward U, and the bias patterns across bases of milRNAs were similar in the aforementioned three groups. There were 253 milRNAs, of which 68 up-and 54 down-regulated milRNAs shared by these groups. Additionally, the expression and sequences of three milRNAs were validated by stem-loop RT-PCR and Sanger sequencing. Trend analysis indicated that 79 DEmilRNAs were classified into three significant profiles (Profile4, Profile6, and Profile7). Target mRNAs of DEmilRNAs in these three significant profiles were engaged in 42 GO terms such as localization, antioxidant activity, and nucleoid. These targets were also involved in 120 KEGG pathways including lysine biosynthesis, pyruvate metabolism, and biosynthesis of antibiotics. Further investigation suggested that DEmilRNA-targeted mRNAs were associated with the MAPK signaling pathway, glycerolipid metabolism, superoxide dismutase, and enzymes related to chitin synthesis and degradation. Moreover, the binding relationships between aap-milR10516-x and ChsD as well as between aap-milR-2478-y and mkh1 were confirmed utilizing a combination of dual-luciferase reporter gene assay and RT-qPCR. Our data not only provide new insights into the A. apis proliferation and invasion, but also lay a basis for illustrating the DEmilRNA-modulated mechanisms underlying the A. apis infection.
Keyword ：
Apis cerana Apis cerana Ascosphaera apis Ascosphaera apis chalkbrood chalkbrood milRNA milRNA regulatory network regulatory network target mRNA target mRNA
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| GB/T 7714 | Liu, Xiaoyu , Geng, Sihai , Ye, Daoyou et al. Global discovery, expression pattern, and regulatory role of miRNA-like RNAs in Ascosphaera apis infecting the Asian honeybee larvae [J]. | FRONTIERS IN MICROBIOLOGY , 2025 , 16 . |
| MLA | Liu, Xiaoyu et al. "Global discovery, expression pattern, and regulatory role of miRNA-like RNAs in Ascosphaera apis infecting the Asian honeybee larvae" . | FRONTIERS IN MICROBIOLOGY 16 (2025) . |
| APA | Liu, Xiaoyu , Geng, Sihai , Ye, Daoyou , Xu, Wenhua , Zheng, Yidi , Wang, Fangji et al. Global discovery, expression pattern, and regulatory role of miRNA-like RNAs in Ascosphaera apis infecting the Asian honeybee larvae . | FRONTIERS IN MICROBIOLOGY , 2025 , 16 . |
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【目的】旨在探究lncRNA17000在意大利蜜蜂Apis mellifera ligustica工蜂不同组织和发育阶段中的表达模式及其调控作用，为进一步的功能和机制研究提供基础。【方法】通过RT-PCR验证lncRNA17000在工蜂不同组织中的表达。采用RT-qPCR检测其不同组织和发育阶段的相对表达量。利用LncTar、Miranda、RNAhybrid和TargetScan等一系列软件分析lncRNA17000的顺式、反式和竞争性内源RNA(Competing endogenous RNA, ceRNA)调控作用。【结果】在工蜂的脑、触角、毒腺、中肠、表皮、咽下腺和脂肪体7个组织中均扩增出符合预期大小（约133bp）的目的片段。LncRNA17000在上述7个组织中差异表达，在触角中的表达量最高且显著高于（P<0.05）脂肪体中的表达量。LncRNA17000在工蜂的卵、幼虫、预蛹、蛹和成虫中差异表达，在7日龄预蛹中的表达量最高且显著高于（P<0.05）3日龄幼虫、8日龄预蛹和12日龄蛹中的表达量。LncRNA17000在6、12、15和18日龄成虫体内的表达量显著低于（P<0.05）1日龄成虫体内的表达量且表达水平随日龄增长而降低。LncRNA17000潜在调控7个上下游基因的转录和2个共表达基因的表达。LncRNA17000可靶向45个miRNA进而靶向66条mRNA，这些靶mRNA涉及23个GO条目和25条KEGG通路。【结论】LncRNA17000在意大利蜜蜂工蜂的不同组织和发育阶段中动态差异表达，在触角和7日龄预蛹中特异性高表达；lncRNA17000可能通过顺式作用调控TFIID亚基11亚型X2基因转录，通过反式作用调控蜜蜂翼视蛋白基因表达，通过ceRNA网络靶向多个miRNA和mRNA进而影响胰岛素、ErbB和mTOR等信号通路。
Keyword ：
lncRNA17000 lncRNA17000 意大利蜜蜂 意大利蜜蜂 时空表达谱 时空表达谱 调控作用 调控作用 长链非编码RNA 长链非编码RNA
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| GB/T 7714 | 康婧 , 任亚萍 , 叶道有 et al. 意大利蜜蜂lncRNA17000的时空表达谱与潜在调控作用 [J]. | 应用昆虫学报 , 2025 , 62 (03) : 764-773 . |
| MLA | 康婧 et al. "意大利蜜蜂lncRNA17000的时空表达谱与潜在调控作用" . | 应用昆虫学报 62 . 03 (2025) : 764-773 . |
| APA | 康婧 , 任亚萍 , 叶道有 , 王宁 , 陈颖 , 李峥源 et al. 意大利蜜蜂lncRNA17000的时空表达谱与潜在调控作用 . | 应用昆虫学报 , 2025 , 62 (03) , 764-773 . |
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本发明涉及教学用具技术领域，具体涉及一种教学系统，包括教学器具，教学器具包括轧光机、轧花机、风机和静电起电机；轧光机和轧花机均包括机架、驱动电机、控制器；机架具有喂入侧，机架包括上挡板和下挡板；上挡板设有第一出风口，下挡板设有第二出风口；第一出风口、第二出风口分别与风机连通；第一出风口、第二出风口上设有多条与静电起电机连接的柔性导电丝；判断柔性导电丝的电荷量是否发生波动，若是则认定为与人体接触则急停；本发明通过利用蜂蜡绝缘、人体导电的特点，结合柔性导电丝配合静电起电机，上下挡板的柔性导电丝带不同电荷与人体发生接触造成电位变化控制驱动电机的急停，进而实现安全的教学演示、规范实操人员的动作。
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| GB/T 7714 | 熊翠玲 , 朱翔杰 , 徐新建 et al. 一种教学系统 : CN202510033682.4[P]. | 2025-01-09 . |
| MLA | 熊翠玲 et al. "一种教学系统" : CN202510033682.4. | 2025-01-09 . |
| APA | 熊翠玲 , 朱翔杰 , 徐新建 , 周姝婧 , 陈大福 . 一种教学系统 : CN202510033682.4. | 2025-01-09 . |
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本发明涉及蜜蜂科学技术领域，具体涉及一种利用蜂蜡制作巢脾的3D打印方法，包括采用加热装置对蜂蜡进行预热，步骤二、将巢脾需要打印的形状提前导入到3D打印机内，巢脾的四侧边沿上分别设置有多个连接边沿和多个缺口；将巢脾的形状切割成多层的打印路径，与连接边沿打印路径为连接段，设定最底层的打印路径的其中一个连接段为起始段；将未打印的巢础框水平放置在打印机上，当打印起始段或连接段时液态蜂蜡挤出量为其他打印路径的1.2‑1.5倍，同时关闭冷却风扇或降低冷却风扇转速；本发明通过加热装置对蜂蜡进行预热，将蜂蜡作为打印的基材，使得蜜蜂在筑巢时无需筑造巢脾，直接可以使用已经打印好的巢脾，无需消耗蜂蜡和时间。
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| GB/T 7714 | 熊翠玲 , 邱剑丰 , 陈大福 et al. 一种利用蜂蜡制作巢脾的3D打印方法 : CN202411765761.6[P]. | 2024-12-04 . |
| MLA | 熊翠玲 et al. "一种利用蜂蜡制作巢脾的3D打印方法" : CN202411765761.6. | 2024-12-04 . |
| APA | 熊翠玲 , 邱剑丰 , 陈大福 , 赵浩东 , 张天泽 , 吴函雨 et al. 一种利用蜂蜡制作巢脾的3D打印方法 : CN202411765761.6. | 2024-12-04 . |
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本研究旨在利用已获得的纳米孔长读段测序数据鉴定和分析意大利蜜蜂Apis mellifera ligustica体液免疫通路相关基因及全长转录本，为深入开展功能研究提供资源与基础。使用Blast工具将鉴定到的所有全长转录本比对Nr数据库以筛选出体液免疫通路相关基因和剪接体。采用gffcompare软件将全长转录本与西方蜜蜂Apis mellifera参考基因组（Amel＿HAv3.1）上注释的转录本进行比较来优化已注释基因的结构。利用TAPIS pipeline预测和分析相关基因的可变多聚腺苷酸化（Alternative polyadenylation,APA）位点，再通过TBtools软件鉴定APA位点上游的基序。使用Astalavista软件鉴定可变剪接（Alternative splicing,AS）事件，进而通过IGV浏览器进行结构可视化。通过RT-PCR验证AS事件的真实性。分别鉴定到Toll信号通路相关的26个基因和41条剪接体，Imd信号通路相关的9个基因和11条剪接体，JNK-MAPK-p38信号通路相关的18个基因和21条剪接体，JAK-STAT信号通路相关的9个基因和13条剪接体。共对西方蜜蜂参考基因组上注释到体液免疫通路的20个基因进行了结构优化，正链和负链结构优化的基因分别有5个和15个，5′端和3′端延长的基因有13个和7个，其中有两个基因(LOC724728;LOC411861)的5′端和3′端都有延长。共鉴定到体液免疫通路相关基因的69次AS事件，包括2次可变3′端剪接（Alternative 3′splice site,A3SS）、17次内含子保留（Intron retention,IR）、14次可变5′端剪接（Alternative 5′splice site,A5SS）和36次外显子跳跃（Exon skipping,ES）。RT-PCR结果显示目的片段大小符合预期，证实了随机选择的6次AS事件的真实性。共鉴定到体液免疫通路相关的40个基因含1个及以上APA位点。在APA位点上游鉴定到多个基序，一致性序列为：GGWRRWRTHAARHTWKSYGAYTTTGGTRTWTCNGSDHRMHTDRYHGMWWS。通过3′RACE证实了2个基因的APA位点真实性。鉴定到意大利蜜蜂体液免疫通路相关的62个基因和86条剪接体，优化了西方蜜蜂体液免疫通路相关的20个基因结构，发掘出体液免疫通路...
Keyword ：
体液免疫 体液免疫 可变剪接 可变剪接 可变多聚腺苷酸化 可变多聚腺苷酸化 意大利蜜蜂 意大利蜜蜂 纳米孔测序 纳米孔测序 西方蜜蜂 西方蜜蜂
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| GB/T 7714 | 宋宇轩 , 张荣华 , 董舒楠 et al. 意大利蜜蜂体液免疫通路相关基因及其剪接分析 [J]. | 环境昆虫学报 , 2025 , 47 (04) : 1226-1237 . |
| MLA | 宋宇轩 et al. "意大利蜜蜂体液免疫通路相关基因及其剪接分析" . | 环境昆虫学报 47 . 04 (2025) : 1226-1237 . |
| APA | 宋宇轩 , 张荣华 , 董舒楠 , 王梦怡 , 范小雪 , 李婧娴 et al. 意大利蜜蜂体液免疫通路相关基因及其剪接分析 . | 环境昆虫学报 , 2025 , 47 (04) , 1226-1237 . |
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【目的】本研究旨在系统鉴定和分析西方蜜蜂Apis mellifera肽聚糖识别蛋白（peptidoglycan recognition protein, PGRP）相关基因及其剪接异构体，探析PGRP基因剪接异构体编码蛋白的理化性质和分子特征，并测定PGRP基因剪接异构体在西方蜜蜂工蜂成虫应答东方蜜蜂微孢子虫Nosema ceranae侵染中的表达模式，以期为西方蜜蜂PGRP基因剪接异构体的功能研究提供参考和依据。【方法】基于已获得的西方蜜蜂8和11日龄工蜂成虫中肠纳米孔测序数据，利用Blast工具将前期鉴定到的所有全长转录本分别比对到Nr和KEGG数据库，筛选出西方蜜蜂PGRP基因及其剪接异构体，并随机选择PGRP基因的5条剪接异构体通过RT-PCR验证序列的真实性。使用Gffcompare软件将鉴定到的PGRP基因序列比对至西方蜜蜂参考基因组以优化基因结构。采用Astalavista软件鉴定PGRP基因的可变剪接（alternative splicing, AS）事件类型，再通过RT-PCR和Sanger测序验证AS事件。利用相关软件预测和分析PGRP基因剪接异构体编码蛋白的理化性质和分子特征。采用RT-qPCR检测东方蜜蜂微孢子虫接种后西方蜜蜂工蜂成虫中肠中PGRP基因剪接异构体的相对表达量。【结果】共鉴定到西方蜜蜂的4个PGRP相关基因（PGRP-3,PGRP-S2,PGRP-LC和PGRP-LB）及其19条剪接异构体。通过RT-PCR与Sanger测序证实了PGRP基因5条剪接异构体（rna14029, ONT.6350.8, ONT.6350.10, rna24089和ONT.6350.7）的表达和序列真实性。PGRP-3（gene10434）的5′和3′端分别延长了8和1 055 bp,PGRP-S2（gene10435）的5′和3′端分别延长了27和234 bp。通过RT-PCR证实了PGRP-S2的AS事件的真实性。剪接异构体ONT.6350.2编码蛋白的分子式为C
Keyword ：
东方蜜蜂微孢子虫 东方蜜蜂微孢子虫 剪接异构体 剪接异构体 纳米孔测序 纳米孔测序 肽聚糖识别蛋白 肽聚糖识别蛋白 西方蜜蜂 西方蜜蜂
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| GB/T 7714 | 冯佩林 , 朱乐冉 , 臧贺 et al. 西方蜜蜂肽聚糖识别蛋白相关基因的剪接异构体鉴定及分析 [J]. | 昆虫学报 , 2025 , 68 (02) : 133-143 . |
| MLA | 冯佩林 et al. "西方蜜蜂肽聚糖识别蛋白相关基因的剪接异构体鉴定及分析" . | 昆虫学报 68 . 02 (2025) : 133-143 . |
| APA | 冯佩林 , 朱乐冉 , 臧贺 , 刘小玉 , 康婧 , 邱剑丰 et al. 西方蜜蜂肽聚糖识别蛋白相关基因的剪接异构体鉴定及分析 . | 昆虫学报 , 2025 , 68 (02) , 133-143 . |
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【目的】本研究旨在通过解析意大利蜜蜂Apis mellifera ligustica lncRNA15837的调控方式和作用，并检测lncRNA15837在工蜂不同发育阶段和成虫不同组织及幼虫应答蜜蜂球囊菌Ascosphaera apis侵染中的表达模式，为深入探究lncRNA15837的调控功能与机制提供参考和依据。【方法】基于已获得的意大利蜜蜂7和10日龄工蜂成虫中肠高质量转录组数据，利用Miranda, RNAhybrid和TargetScan软件预测lncRNA15837靶向的miRNA及miRNA靶向的mRNA,再通过Cytoscape v3.7.1软件构建并可视化竞争性内源RNA (competing endogenous RNA, ceRNA)调控网络。使用Blast工具将靶mRNA比对至GO和KEGG数据库以获得相应的功能和通路注释。通过RT-PCR验证lncRNA15837在刚出房工蜂成虫不同组织(触角、咽下腺、脑、表皮、中肠、脂肪体和毒腺)中的表达。利用RT-qPCR检测lncRNA15837在工蜂的不同发育阶段(卵、3日龄幼虫、 1和2日龄预蛹、 4日龄蛹及1, 2, 6, 12, 15和18日龄工蜂成虫)、刚出房工蜂成虫不同组织(触角、咽下腺、脑、表皮、中肠、脂肪体和毒腺)和3日龄幼虫感染蜜蜂球囊菌后4, 5和6日龄幼虫肠道中的表达量。【结果】LncRNA15837可靶向ame-miR-21-x和ame-miR-0046-3p等29个miRNA,进而靶向1 559条mRNA,形成1个复杂的ceRNA网络；上述靶mRNA可注释到436个GO条目，其中114条靶mRNA涉及139个分子功能相关条目，115条靶mRNA涉及257个生物学进程相关条目，67条靶mRNA涉及40个细胞组分相关条目；可注释到224条KEGG通路，其中28条靶mRNA注释到内吞、吞噬细胞和溶酶体等细胞免疫通路，以及157条靶mRNA注释到JAK-STAT, Toll和Imd, NF-kappa B和Toll样受体信号通路等体液免疫通路；lncRNA15837在工蜂卵、幼虫、预蛹、蛹和成虫中差异表达，在1日龄预蛹中的表达量最低，而在3日龄幼虫中的表达量最高；lncRNA15837在刚出房工蜂成虫的触角、毒腺、脑、中肠、咽下腺、脂肪体和表皮中均有表达但表达量存在差异，在脂肪体中的表达量最低，而在毒腺中的表达量最高；相较于未接...
Keyword ：
意大利蜜蜂 意大利蜜蜂 时空表达谱 时空表达谱 竞争性内源RNA 竞争性内源RNA 蜜蜂球囊菌 蜜蜂球囊菌 长链非编码RNA 长链非编码RNA
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| GB/T 7714 | 宋宇轩 , 李坤泽 , 臧贺 et al. 意大利蜜蜂lncRNA15837的调控作用和表达模式分析 [J]. | 昆虫学报 , 2025 , 68 (02) : 144-153 . |
| MLA | 宋宇轩 et al. "意大利蜜蜂lncRNA15837的调控作用和表达模式分析" . | 昆虫学报 68 . 02 (2025) : 144-153 . |
| APA | 宋宇轩 , 李坤泽 , 臧贺 , 刘小玉 , 吴陶 , 徐细建 et al. 意大利蜜蜂lncRNA15837的调控作用和表达模式分析 . | 昆虫学报 , 2025 , 68 (02) , 144-153 . |
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【目的】本研究旨在明确意大利蜜蜂Apis mellifera ligustica piR-ame-1186994的调控功能，为进一步探究piR-ame-1186994的调控作用机制提供科学依据。【方法】通过Stem-loop RT-PCR和Sanger测序分别验证piR-ame-1186994在意大利蜜蜂6日龄工蜂成虫中肠、 12日龄雄蜂成虫精巢和7日龄蜂王成虫卵巢中的表达和序列真实性。使用相关软件预测piR-ame-1186994的靶mRNA并进行GO和KEGG数据库注释，进而构建发育信号通路、能量代谢通路及细胞和体液免疫通路相关调控网络。饲喂刚出房工蜂成虫piR-ame-1186994的模拟物(mimic)和mimic-NC(阴性对照组),利用RT-qPCR检测工蜂成虫中肠中的piR-ame-1186994及其关键2个关键靶基因(YAP1和PLD2)的相对表达量。【结果】在意大利蜜蜂6日龄工蜂成虫中肠、 12日龄雄蜂成虫精巢和7日龄蜂王成虫卵巢中均扩增出piR-ame-1186994的特异性片段。piR-ame-1186994共靶向1 097条mRNA,可注释到新陈代谢过程、结合和细胞等30条GO条目以及Wnt信号通路、内吞作用和催产素信号通路等182条KEGG通路。其中，分别有36和16条靶mRNA涉及5条发育相关信号通路(mTOR, Wnt, Hippo, AMPK和Notch信号通路)和4条能量代谢相关通路(氨基糖和核苷酸糖代谢、果糖和甘露糖代谢、糖酵解/糖异生及磷酸戊糖通路)。此外，分别有29和8条靶mRNA涉及4条细胞免疫通路(溶酶体、内吞作用、吞噬体和泛素介导的蛋白水解)和3条体液免疫通路(PI3K-Akt, MAPK和FoxO信号通路)。相较于阴性对照组mimic-NC, mimic-piR组中piR-ame-1186994的表达量在1, 3和5日龄工蜂中肠中显著上调，在2和4日龄工蜂中肠中极显著上调；靶基因YAP1的表达量在1, 3, 4和5日龄工蜂中肠中极显著下调，在2日龄工蜂中肠中显著下调；靶基因PLD2的表达量在2, 3和5日龄工蜂中肠中显著下调，在4日龄的工蜂中肠中极显著下调。【结论】piR-ame-1186994在意大利蜜蜂工蜂中肠、雄蜂精巢和蜂王卵巢中存在与表达；piR-ame-1186994通过靶向和负调控YAP1和PLD2的表达潜在调节工蜂中肠发育和免疫。
Keyword ：
piRNA piRNA 中肠 中肠 卵巢 卵巢 意大利蜜蜂 意大利蜜蜂 精巢 精巢
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| GB/T 7714 | 张艺琼 , 那志豪 , 李琪明 et al. 意大利蜜蜂piR-ame-1186994的靶基因与功能分析 [J]. | 昆虫学报 , 2025 , 68 (03) : 260-270 . |
| MLA | 张艺琼 et al. "意大利蜜蜂piR-ame-1186994的靶基因与功能分析" . | 昆虫学报 68 . 03 (2025) : 260-270 . |
| APA | 张艺琼 , 那志豪 , 李琪明 , 王梦怡 , 李婧娴 , 代梦远 et al. 意大利蜜蜂piR-ame-1186994的靶基因与功能分析 . | 昆虫学报 , 2025 , 68 (03) , 260-270 . |
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