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学者姓名：陈大福
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Abstract ：
本发明涉及教学用具技术领域，具体涉及一种教学系统，包括教学器具，教学器具包括轧光机、轧花机、风机和静电起电机；轧光机和轧花机均包括机架、驱动电机、控制器；机架具有喂入侧，机架包括上挡板和下挡板；上挡板设有第一出风口，下挡板设有第二出风口；第一出风口、第二出风口分别与风机连通；第一出风口、第二出风口上设有多条与静电起电机连接的柔性导电丝；判断柔性导电丝的电荷量是否发生波动，若是则认定为与人体接触则急停；本发明通过利用蜂蜡绝缘、人体导电的特点，结合柔性导电丝配合静电起电机，上下挡板的柔性导电丝带不同电荷与人体发生接触造成电位变化控制驱动电机的急停，进而实现安全的教学演示、规范实操人员的动作。
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| GB/T 7714 | 熊翠玲 , 朱翔杰 , 徐新建 et al. 一种教学系统 : CN202510033682.4[P]. | 2025-01-09 . |
| MLA | 熊翠玲 et al. "一种教学系统" : CN202510033682.4. | 2025-01-09 . |
| APA | 熊翠玲 , 朱翔杰 , 徐新建 , 周姝婧 , 陈大福 . 一种教学系统 : CN202510033682.4. | 2025-01-09 . |
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本发明涉及蜜蜂科学技术领域，具体涉及一种利用蜂蜡制作巢脾的3D打印方法，包括采用加热装置对蜂蜡进行预热，步骤二、将巢脾需要打印的形状提前导入到3D打印机内，巢脾的四侧边沿上分别设置有多个连接边沿和多个缺口；将巢脾的形状切割成多层的打印路径，与连接边沿打印路径为连接段，设定最底层的打印路径的其中一个连接段为起始段；将未打印的巢础框水平放置在打印机上，当打印起始段或连接段时液态蜂蜡挤出量为其他打印路径的1.2‑1.5倍，同时关闭冷却风扇或降低冷却风扇转速；本发明通过加热装置对蜂蜡进行预热，将蜂蜡作为打印的基材，使得蜜蜂在筑巢时无需筑造巢脾，直接可以使用已经打印好的巢脾，无需消耗蜂蜡和时间。
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| GB/T 7714 | 熊翠玲 , 邱剑丰 , 陈大福 et al. 一种利用蜂蜡制作巢脾的3D打印方法 : CN202411765761.6[P]. | 2024-12-04 . |
| MLA | 熊翠玲 et al. "一种利用蜂蜡制作巢脾的3D打印方法" : CN202411765761.6. | 2024-12-04 . |
| APA | 熊翠玲 , 邱剑丰 , 陈大福 , 赵浩东 , 张天泽 , 吴函雨 et al. 一种利用蜂蜡制作巢脾的3D打印方法 : CN202411765761.6. | 2024-12-04 . |
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肠道菌群作为一个复杂的微生物生态系统，可以通过调节宿主的免疫系统从而来影响宿主的健康，在宿主的众多生命活动中扮演着重要的角色。然而，对许多重要的昆虫（如蜜蜂）来说，肠道菌群与宿主免疫功能之间的关系仍不清楚。文章对肠道菌群在蜜蜂天然免疫方面的研究情况进行了简要概述，介绍了蜜蜂肠道菌群的组成情况，阐述了其对蜜蜂抗菌肽表达、免疫相关通路的影响以及在抵御病原体侵染等方面发挥的作用，探讨了当前研究存在的局限以及未来研究方向，旨在为深入理解蜜蜂与肠道菌群的相互作用以及蜜蜂健康养殖等方面提供参考依据，为害虫防治和有益昆虫的保护提供新的策略。
Keyword ：
天然免疫 天然免疫 抗菌肽 抗菌肽 昆虫 昆虫 肠道菌群 肠道菌群 蜜蜂 蜜蜂
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| GB/T 7714 | 吴鹰 , 牛庆生 , 兰凤明 et al. 肠道菌群调节蜜蜂天然免疫的研究进展 [J]. | 蜜蜂杂志 , 2025 , 45 (03) : 1-6 . |
| MLA | 吴鹰 et al. "肠道菌群调节蜜蜂天然免疫的研究进展" . | 蜜蜂杂志 45 . 03 (2025) : 1-6 . |
| APA | 吴鹰 , 牛庆生 , 兰凤明 , 郝京玉 , 郭睿 , 陈大福 et al. 肠道菌群调节蜜蜂天然免疫的研究进展 . | 蜜蜂杂志 , 2025 , 45 (03) , 1-6 . |
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Synthetic biology and nanotechnology fusion represent a transformative approach promoting fundamental and clinical biomedical science development. In SynBioNanoDesign, biological systems are reimagined as dynamic and programmable materials to yield engineered nanomaterials with emerging and specific functionalities. This review elucidates a comprehensive examination of synthetic biology's pivotal role in advancing engineered nanomaterials for targeted drug delivery systems. It begins with exploring the fundamental synergy between synthetic biology and nanotechnology, then highlights the current landscape of nanomaterials in targeted drug delivery applications. Subsequently, the review discusses the design of novel nanomaterials informed by biological principles, focusing on expounding the synthetic biology tools and the potential for developing advanced nanomaterials. Afterward, the research advances of innovative materials design through synthetic biology were systematically summarized, emphasizing the integration of genetic circuitry to program nanomaterial responses. Furthermore, the challenges, current weaknesses and opportunities, prospective directions, and ethical and societal implications of SynBioNanoDesign in drug delivery are elucidated. Finally, the review summarizes the transformative impact that synthetic biology may have on drug-delivery technologies in the future.
Keyword ：
Engineered nanomaterials Engineered nanomaterials Genetic circuitry Genetic circuitry Nanobiotechnology Nanobiotechnology Personalized medicine Personalized medicine Regulatory challenges Regulatory challenges Synthetic biology Synthetic biology Targeted drug delivery Targeted drug delivery
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| GB/T 7714 | Cai, Qian , Guo, Rui , Chen, Dafu et al. SynBioNanoDesign: pioneering targeted drug delivery with engineered nanomaterials [J]. | JOURNAL OF NANOBIOTECHNOLOGY , 2025 , 23 (1) . |
| MLA | Cai, Qian et al. "SynBioNanoDesign: pioneering targeted drug delivery with engineered nanomaterials" . | JOURNAL OF NANOBIOTECHNOLOGY 23 . 1 (2025) . |
| APA | Cai, Qian , Guo, Rui , Chen, Dafu , Deng, Zixin , Gao, Jiangtao . SynBioNanoDesign: pioneering targeted drug delivery with engineered nanomaterials . | JOURNAL OF NANOBIOTECHNOLOGY , 2025 , 23 (1) . |
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Ascosphaera apis, a specialized fungal pathogen, causes lethal infection in honeybee larvae. miRNA-like small RNAs (milRNAs) are fungal small non-coding RNAs similar to miRNAs, which have been shown to regulate fungal hyphal growth, spore formation, and pathogenesis. Based on the transcriptome data, differentially expressed miRNA-like RNAs (DEmilRNAs) in A. apis infecting the Apis cerana cerana worker 4-, 5-, and 6-day-old larvae (Aa-4, Aa-5, and Aa-6) were screened and subjected to trend analysis, followed by target prediction and annotation as well as investigation of regulatory networks, with a focus on sub-networks relative to MAPK signaling pathway, glycerolipid metabolism, superoxide dismutase, and enzymes related to chitin synthesis and degradation. A total of 606 milRNAs, with a length distribution ranging from 18 nt to 25 nt, were identified. The first nucleotide of these milRNAs presented a bias toward U, and the bias patterns across bases of milRNAs were similar in the aforementioned three groups. There were 253 milRNAs, of which 68 up-and 54 down-regulated milRNAs shared by these groups. Additionally, the expression and sequences of three milRNAs were validated by stem-loop RT-PCR and Sanger sequencing. Trend analysis indicated that 79 DEmilRNAs were classified into three significant profiles (Profile4, Profile6, and Profile7). Target mRNAs of DEmilRNAs in these three significant profiles were engaged in 42 GO terms such as localization, antioxidant activity, and nucleoid. These targets were also involved in 120 KEGG pathways including lysine biosynthesis, pyruvate metabolism, and biosynthesis of antibiotics. Further investigation suggested that DEmilRNA-targeted mRNAs were associated with the MAPK signaling pathway, glycerolipid metabolism, superoxide dismutase, and enzymes related to chitin synthesis and degradation. Moreover, the binding relationships between aap-milR10516-x and ChsD as well as between aap-milR-2478-y and mkh1 were confirmed utilizing a combination of dual-luciferase reporter gene assay and RT-qPCR. Our data not only provide new insights into the A. apis proliferation and invasion, but also lay a basis for illustrating the DEmilRNA-modulated mechanisms underlying the A. apis infection.
Keyword ：
Apis cerana Apis cerana Ascosphaera apis Ascosphaera apis chalkbrood chalkbrood milRNA milRNA regulatory network regulatory network target mRNA target mRNA
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| GB/T 7714 | Liu, Xiaoyu , Geng, Sihai , Ye, Daoyou et al. Global discovery, expression pattern, and regulatory role of miRNA-like RNAs in Ascosphaera apis infecting the Asian honeybee larvae [J]. | FRONTIERS IN MICROBIOLOGY , 2025 , 16 . |
| MLA | Liu, Xiaoyu et al. "Global discovery, expression pattern, and regulatory role of miRNA-like RNAs in Ascosphaera apis infecting the Asian honeybee larvae" . | FRONTIERS IN MICROBIOLOGY 16 (2025) . |
| APA | Liu, Xiaoyu , Geng, Sihai , Ye, Daoyou , Xu, Wenhua , Zheng, Yidi , Wang, Fangji et al. Global discovery, expression pattern, and regulatory role of miRNA-like RNAs in Ascosphaera apis infecting the Asian honeybee larvae . | FRONTIERS IN MICROBIOLOGY , 2025 , 16 . |
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Apoptosis is a genetically controlled process vital for homeostasis. This study examined the apoptotic response in the gut of Apis mellifera (A. mellifera) larvae to infection by Ascosphaera apis (A. apis) and its impact on host resistance and pathogen virulence. Here, Worker larvae of A. mellifera were inoculated with purified A. apis spores. We then quantified the expression of key apoptosis-related genes (AmCaspase-3, AmBax, and AmBcl-2) in the host gut and detected apoptotic cells via TUNEL assay. To functionally assess the role of apoptosis, larvae were treated with either the apoptosis inhibitor Z-VAD-FMK or the activator PAC-1, after which host survival, expression of apoptosis-associated genes, and the fungal virulence factor gene Ste11-like were analyzed. Our results showed that infection with A. apis significantly upregulated the expression of AmCaspase-3 and AmBax (p < 0.05) at 1-3 days post-inoculation (dpi), while the expression of AmBcl-2 was significantly reduced at 1 and 3 dpi (p < 0.05). Consistent with this, TUNEL assays revealed a markedly stronger green fluorescence signal in the guts of inoculated larvae at 3 dpi compared to uninfected controls, with clear co-localization of TUNEL and nuclear signals, confirming increased apoptosis. Pharmacological inhibition of apoptosis significantly enhanced the survival rate of A. apis-infected larvae, whereas apoptosis activation decreased larval survival. Accordingly, inhibiting apoptosis significantly suppressed the expression of AmCaspase-3 and AmBax (p < 0.001) and upregulated AmBcl-2 (p < 0.001). Conversely, apoptosis activation upregulated AmCaspase-3 (p > 0.05) and AmBax (p < 0.001), while significantly down-regulating AmBcl-2. Furthermore, apoptosis inhibition significantly down-regulated the fungal virulence gene Ste11-like, while its activation had the opposite effect. In summary, A. apis infection induces apoptosis in the larval gut by activating AmCaspase-3 and AmBax and suppressing AmBcl-2. Inhibiting this apoptotic response enhanced host survival by modulating the expression of host apoptosis-related genes and the fungal Ste11-like virulence factor. These findings provide new insights into the host response to A. apis and suggest a potential strategy for controlling chalkbrood disease.
Keyword ：
Apis mellifera Apis mellifera apoptosis apoptosis Ascosphaera apis Ascosphaera apis infection infection survival survival TUNEL TUNEL
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| GB/T 7714 | Zhang, Tianze , Li, Jingxian , Yang, Jiarun et al. Apoptosis and Relevant Genes Are Engaged in the Response of Apis mellifera Larvae to Ascosphaera apis Invasion [J]. | INSECTS , 2025 , 16 (9) . |
| MLA | Zhang, Tianze et al. "Apoptosis and Relevant Genes Are Engaged in the Response of Apis mellifera Larvae to Ascosphaera apis Invasion" . | INSECTS 16 . 9 (2025) . |
| APA | Zhang, Tianze , Li, Jingxian , Yang, Jiarun , Fan, Xiaoxue , Mi, Shiyu , Guo, Xi et al. Apoptosis and Relevant Genes Are Engaged in the Response of Apis mellifera Larvae to Ascosphaera apis Invasion . | INSECTS , 2025 , 16 (9) . |
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【目的】对西方蜜蜂（Apis mellifera）的丝氨酸/苏氨酸蛋白激酶（AmSTPKD3）进行生物信息学分析，测定AmSTPKD3基因在西方蜜蜂工蜂的组织和发育阶段的表达模式，为AmSTPKD3的功能研究提供科学依据。【方法】利用相关生物信息学软件，预测AmSTPKD3的理化性质和分子特征，鉴定西方蜜蜂和其他物种(黑大蜜蜂（Apis laboriosa）、欧洲雄蜂（Bombus terrestris）、大蜜蜂（Apis dorsata）、小蜜蜂（Apis florea）、田野熊蜂（Bombus pascuorum）、东方蜜蜂（Apis cerana）、美洲东部熊蜂（Bombus impatiens）和巴西无刺蜜蜂（Frieseomelitta varia）)STPKD3的结构域和保守基序。通过Mega 11.0软件对西方蜜蜂和其他物种的STPKD3进行系统进化分析。采用RT-qPCR方法，检测AmSTPKD3在西方蜜蜂不同组织（触角、毒腺、脑、中肠、脂肪体、表皮和咽下腺）和发育阶段（卵、3日龄幼虫、7和8日龄预蛹、12日龄蛹及1,2,6,12,15和18日龄成虫）的相对表达量。【结果】AmSTPKD3包含3 269个核苷酸，可编码831个氨基酸。AmSTPKD3的分子式为C
Keyword ：
丝氨酸/苏氨酸蛋白激酶 丝氨酸/苏氨酸蛋白激酶 分子特征 分子特征 时空表达谱 时空表达谱 理化性质 理化性质 系统进化 系统进化 西方蜜蜂 西方蜜蜂
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| GB/T 7714 | 董舒楠 , 邹培缘 , 任亚萍 et al. 西方蜜蜂AmSTPKD3蛋白的分子特征与基因表达模式 [J]. | 西北农林科技大学学报(自然科学版) , 2025 , 53 (06) : 29-36 . |
| MLA | 董舒楠 et al. "西方蜜蜂AmSTPKD3蛋白的分子特征与基因表达模式" . | 西北农林科技大学学报(自然科学版) 53 . 06 (2025) : 29-36 . |
| APA | 董舒楠 , 邹培缘 , 任亚萍 , 杜丽婷 , 李坤泽 , 臧贺 et al. 西方蜜蜂AmSTPKD3蛋白的分子特征与基因表达模式 . | 西北农林科技大学学报(自然科学版) , 2025 , 53 (06) , 29-36 . |
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【目的】旨在探究lncRNA17000在意大利蜜蜂Apis mellifera ligustica工蜂不同组织和发育阶段中的表达模式及其调控作用，为进一步的功能和机制研究提供基础。【方法】通过RT-PCR验证lncRNA17000在工蜂不同组织中的表达。采用RT-qPCR检测其不同组织和发育阶段的相对表达量。利用LncTar、Miranda、RNAhybrid和TargetScan等一系列软件分析lncRNA17000的顺式、反式和竞争性内源RNA(Competing endogenous RNA, ceRNA)调控作用。【结果】在工蜂的脑、触角、毒腺、中肠、表皮、咽下腺和脂肪体7个组织中均扩增出符合预期大小（约133bp）的目的片段。LncRNA17000在上述7个组织中差异表达，在触角中的表达量最高且显著高于（P<0.05）脂肪体中的表达量。LncRNA17000在工蜂的卵、幼虫、预蛹、蛹和成虫中差异表达，在7日龄预蛹中的表达量最高且显著高于（P<0.05）3日龄幼虫、8日龄预蛹和12日龄蛹中的表达量。LncRNA17000在6、12、15和18日龄成虫体内的表达量显著低于（P<0.05）1日龄成虫体内的表达量且表达水平随日龄增长而降低。LncRNA17000潜在调控7个上下游基因的转录和2个共表达基因的表达。LncRNA17000可靶向45个miRNA进而靶向66条mRNA，这些靶mRNA涉及23个GO条目和25条KEGG通路。【结论】LncRNA17000在意大利蜜蜂工蜂的不同组织和发育阶段中动态差异表达，在触角和7日龄预蛹中特异性高表达；lncRNA17000可能通过顺式作用调控TFIID亚基11亚型X2基因转录，通过反式作用调控蜜蜂翼视蛋白基因表达，通过ceRNA网络靶向多个miRNA和mRNA进而影响胰岛素、ErbB和mTOR等信号通路。
Keyword ：
lncRNA17000 lncRNA17000 意大利蜜蜂 意大利蜜蜂 时空表达谱 时空表达谱 调控作用 调控作用 长链非编码RNA 长链非编码RNA
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| GB/T 7714 | 康婧 , 任亚萍 , 叶道有 et al. 意大利蜜蜂lncRNA17000的时空表达谱与潜在调控作用 [J]. | 应用昆虫学报 , 2025 , 62 (03) : 764-773 . |
| MLA | 康婧 et al. "意大利蜜蜂lncRNA17000的时空表达谱与潜在调控作用" . | 应用昆虫学报 62 . 03 (2025) : 764-773 . |
| APA | 康婧 , 任亚萍 , 叶道有 , 王宁 , 陈颖 , 李峥源 et al. 意大利蜜蜂lncRNA17000的时空表达谱与潜在调控作用 . | 应用昆虫学报 , 2025 , 62 (03) , 764-773 . |
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【目的】丰富蜜蜂球囊菌（Ascosphaera apis）几丁质酶3（AaCHIT3）的理化性质和分子特征信息，为AaCHIT3基因的功能研究提供科学依据。【方法】利用Expasy网站的ProtParam和ProtScale软件分别预测AaCHIT3蛋白的理化性质和亲水性，采用MEME软件鉴定保守基序和结构域，通过Mega 11.0软件对蜜蜂球囊菌及其他真菌的CHIT3进行系统进化分析。使用RT-qPCR检测蜜蜂球囊菌侵染意大利蜜蜂（Apis mellifera ligustica）工蜂幼虫过程中AaCHIT3的表达模式。【结果】AaCHIT3由372个氨基酸组成，分子质量约为43.03 ku,理论等电点为5.05,分子式为C
Keyword ：
几丁质 几丁质 几丁质酶 几丁质酶 分子特征 分子特征 意大利蜜蜂 意大利蜜蜂 蜜蜂球囊菌 蜜蜂球囊菌 蜜蜂白垩病 蜜蜂白垩病 表达模式 表达模式
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| GB/T 7714 | 王梦怡 , 吴陶 , 范小雪 et al. 蜜蜂球囊菌几丁质酶3基因的生物信息学和表达模式分析 [J]. | 福建农林大学学报(自然科学版) , 2025 , 54 (05) : 640-646 . |
| MLA | 王梦怡 et al. "蜜蜂球囊菌几丁质酶3基因的生物信息学和表达模式分析" . | 福建农林大学学报(自然科学版) 54 . 05 (2025) : 640-646 . |
| APA | 王梦怡 , 吴陶 , 范小雪 , 胡艳雯 , 王炜 , 郑一荻 et al. 蜜蜂球囊菌几丁质酶3基因的生物信息学和表达模式分析 . | 福建农林大学学报(自然科学版) , 2025 , 54 (05) , 640-646 . |
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本研究对西方蜜蜂Apis mellifera细胞周期蛋白依赖性激酶抑制因子1（Cyclin dependent kinase inhibitor1,CKI1）基因AmCKI1进行表达验证，并解析AmCKI1蛋白的理化性质和分子特征，进而测定工蜂不同组织和发育阶段AmCKI1的表达谱，旨在为进一步的功能研究提供参考和基础。通过PCR和Sanger测序验证AmCKI1的表达。使用相关软件预测AmCKI1的理化性质和分子特征，并进行系统进化分析。采用RT-qPCR检测AmCKI1在工蜂的触角、脑、咽下腺、中肠、毒腺、表皮和脂肪体等7个不同组织，卵、幼虫、预蛹、蛹和1日龄、2日龄、6日龄、12日龄、15日龄和17日龄成虫中的相对表达量。结果显示，AmCKI1在工蜂中真实表达。AmCKI1的分子式为C
Keyword ：
分子特征 分子特征 系统进化 系统进化 细胞周期蛋白依赖性激酶抑制因子1 细胞周期蛋白依赖性激酶抑制因子1 表达谱 表达谱 西方蜜蜂 西方蜜蜂
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| GB/T 7714 | 邹培缘 , 臧贺 , 董舒楠 et al. 西方蜜蜂CKI1蛋白的分子特征和基因时空表达谱 [J]. | 环境昆虫学报 , 2025 , 47 (05) : 1579-1586 . |
| MLA | 邹培缘 et al. "西方蜜蜂CKI1蛋白的分子特征和基因时空表达谱" . | 环境昆虫学报 47 . 05 (2025) : 1579-1586 . |
| APA | 邹培缘 , 臧贺 , 董舒楠 , 叶道有 , 杜丽婷 , 邱剑丰 et al. 西方蜜蜂CKI1蛋白的分子特征和基因时空表达谱 . | 环境昆虫学报 , 2025 , 47 (05) , 1579-1586 . |
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