Query：
学者姓名：李江红
Refining：
Year
Type
Indexed by
Source
Complex
Co-Author
Language
Clean All
Abstract ：
High temperature is normally harmful to an organism. However, honey bees evolve, maintaining a relatively higher colony temperature of 34.5 degrees C in the long reproduction period. To determine the effect of such a higher colony temperature on adult bees and its biological significance, newly emerged bees were reared in cages at 34.5 degrees C and room temperature of 25.0 degrees C, respectively. Their survival rate, head weight, royal jelly-secreting gene expression, and morphology of the hypopharyngeal gland were investigated. Moreover, 40-day-old bees with significant differences in survival rate between the two temperature groups were subject to transcriptome and lipidome analysis. The result showed that the higher colony temperature was overall negative for the bees' longevity. Transcriptome analysis showed that fatty acid metabolism-related items were enriched and the involved genes were upregulated in honey bees reared at 34.5 degrees C compared with the honey bees reared at 25.0 degrees C. Lipidomic analysis further validated that fatty acid metabolism, especially sphingolipid metabolism, was significantly altered. Such upregulation of fatty acid metabolism-related genes was also detected in young adult bees of 5 days old reared at 34.5 degrees C. These bees had heavier head weights, higher expression of royal jelly-secreting-related genes, and more developed hypopharyngeal glands. Such results showed that the colony temperature of 34.5 degrees C could accelerate the development process of newly emerged bees to be nurse bees, significantly increasing the colony nurturing capability, which in turn increased the development speed, size, and survivability of the colony. Thereby, the colony temperature of 34.5 degrees C shortened the lifespan of individual bees, but obtained huge returns at the colony level, with remarkable biological significance.
Keyword ：
adult bees adult bees colony temperature colony temperature lipid metabolism lipid metabolism longevity longevity nurturing capability nurturing capability
Cite：
Copy from the list or Export to your reference management。
| GB/T 7714 | Sun, Chaoxia , Huang, Hongji , Yang, Mei et al. The Trade-Off Between the Increased Colony Nurturing Ability and the Decreased Lifespan of Worker Bees (Apis mellifera) [J]. | INSECTS , 2025 , 16 (6) . |
| MLA | Sun, Chaoxia et al. "The Trade-Off Between the Increased Colony Nurturing Ability and the Decreased Lifespan of Worker Bees (Apis mellifera)" . | INSECTS 16 . 6 (2025) . |
| APA | Sun, Chaoxia , Huang, Hongji , Yang, Mei , Ma, Guoshuai , Huang, Xinyao , Huang, Shaokang et al. The Trade-Off Between the Increased Colony Nurturing Ability and the Decreased Lifespan of Worker Bees (Apis mellifera) . | INSECTS , 2025 , 16 (6) . |
| Export to | NoteExpress RIS BibTex |
Version ：
Abstract ：
Propolis contains abundant flavonoid and phenolic compounds, whose composition and concentration vary significantly in different geographical origins, thereby affecting its bioactive properties including anti-inflammatory, antioxidant, and antimicrobial activities. In this study, the flavonoid and phenolic content in the ethanol extract of propolis (EEP) from Henan (HN) and Shandong (SD) provinces was quantitatively analyzed, and the results showed that concentrations of both bioactive components in HN were slightly higher than those in SD. The non-targeted metabolomics technology was further employed to analyze the components of EEP, and a total of 10683 metabolites were detected. In the comparison between the samples of HN and SD, there were a total of 1436 differential metabolites, with 553 decreased and 883 increased in the HN sample. Among them, there were 205 differential metabolites related to flavonoids and phenols, with 108 decreased and 97 increased in the HN sample. However, a greater number of carboxylic acids and derivatives, fatty derivatives and organooxygen metabolites were found at higher relative levels in the HN sample. As a result, the EEP of the HN sample was selected to investigate its inhibitory effect on inflammation in lipopolysaccharide (LPS)-induced BV2 microglia cells. The results showed that LPS promoted the M1 polarization of BV2 microglia. However, treatment with EEP at concentrations of 10 mu g/mL, 5 mu g/mL, and 2.5 mu g/mL could partially restore the cell morphology to its non-activated state. Meanwhile, LPS stimulation increased the protein levels of IL-1 beta, IL-6 and TNF-alpha significantly, as well as the relative gene expression levels of IL-1 beta, IL-6, TNF-alpha, COX-2, iNOS and TLR4. After treatment with the EEP, the expression levels of these three proteins and six genes were significantly decreased. These findings revealed that EEP effectively inhibited the M1 polarization of LPS-induced BV2 cells and decreased the expression of inflammatory factors, indicating its potential as a therapeutic agent for neuroinflammation.
Keyword ：
anti-inflammatory anti-inflammatory flavonoids flavonoids metabolomics metabolomics microglia microglia phenols phenols propolis propolis
Cite：
Copy from the list or Export to your reference management。
| GB/T 7714 | Xu, Xiaolan , Li, Chunxia , Zhu, Yuxuan et al. Non-Targeted Metabolomic Analysis of Ethanol Extract of Propolis and Its Anti-Inflammatory Effects in LPS-Induced BV2 Microglial Cells via the TLR4 Signaling Pathway [J]. | NUTRIENTS , 2025 , 17 (17) . |
| MLA | Xu, Xiaolan et al. "Non-Targeted Metabolomic Analysis of Ethanol Extract of Propolis and Its Anti-Inflammatory Effects in LPS-Induced BV2 Microglial Cells via the TLR4 Signaling Pathway" . | NUTRIENTS 17 . 17 (2025) . |
| APA | Xu, Xiaolan , Li, Chunxia , Zhu, Yuxuan , Zhao, Shuangshuang , Wu, Fangjing , He, Qian et al. Non-Targeted Metabolomic Analysis of Ethanol Extract of Propolis and Its Anti-Inflammatory Effects in LPS-Induced BV2 Microglial Cells via the TLR4 Signaling Pathway . | NUTRIENTS , 2025 , 17 (17) . |
| Export to | NoteExpress RIS BibTex |
Version ：
Abstract ：
Honey bees (Apis mellifera ligustica) are essential pollinators in both ecosystems and agricultural production. However, their populations are declining due to various factors, including pesticide exposure. Despite their importance, the reproductive castes, particularly drones, remain understudied in terms of pesticide effects. To investigate the effects of azoxystrobin and picoxystrobin on honey bee drones, the drones were exposed to different concentrations of azoxystrobin and picoxystrobin for 14 days; the drone survival, body weight, nutrient content, reproductive organs, and sperm concentration were assessed. Results showed that exposure to both fungicides caused a significant reduction in drone survival rates, with survival rates decreasing progressively as the duration of exposure increased. Compared to the control group, the body weights of drones in all treatment groups were significantly lower on days 7 and 14. Nutrient analysis revealed that low concentrations of azoxystrobin and picoxystrobin increased protein levels, while free fatty acid content decreased significantly in all treatment groups. No significant changes were observed in the total carbohydrate content. Morphological examination of reproductive organs showed that the lengths of the mucus glands and seminal vesicles in drones were significantly shorter in the treatment groups compared to the control group. Furthermore, exposure to azoxystrobin and picoxystrobin resulted in a significant decline in sperm concentration in the drones. These findings indicate that azoxystrobin and picoxystrobin have adverse effects on the health and reproductive capacity of honey bee drones. The present study highlights the need to reassess the risks posed by these fungicides to pollinators, particularly given the critical role of drones in maintaining the genetic diversity and resilience of honey bee colonies. Further research is warranted to elucidate the underlying mechanisms of these effects and explore potential mitigation strategies.
Keyword ：
Apis mellifera ligustica Apis mellifera ligustica azoxystrobin azoxystrobin drone drone pesticide exposure pesticide exposure picoxystrobin picoxystrobin reproductive ability reproductive ability
Cite：
Copy from the list or Export to your reference management。
| GB/T 7714 | Tong, Wenlong , Wang, Lizhu , Tao, Bingfang et al. Azoxystrobin and Picoxystrobin Lead to Decreased Fitness of Honey Bee Drones (Apis mellifera ligustica) [J]. | AGRICULTURE-BASEL , 2025 , 15 (15) . |
| MLA | Tong, Wenlong et al. "Azoxystrobin and Picoxystrobin Lead to Decreased Fitness of Honey Bee Drones (Apis mellifera ligustica)" . | AGRICULTURE-BASEL 15 . 15 (2025) . |
| APA | Tong, Wenlong , Wang, Lizhu , Tao, Bingfang , Yao, Huanjing , Liu, Huiping , Huang, Shaokang et al. Azoxystrobin and Picoxystrobin Lead to Decreased Fitness of Honey Bee Drones (Apis mellifera ligustica) . | AGRICULTURE-BASEL , 2025 , 15 (15) . |
| Export to | NoteExpress RIS BibTex |
Version ：
Abstract ：
Fungal diseases of agricultural crops cause severe economic losses to the growers. For the control of these diseases, azoxystrobin is one of the recommended fungicides. This fungicide is systemic in action and is expected to reach the floral part of the treated crop and its residue in the pollen and nectar, the natural food sources of honey bees, which could be collected and fed on by honey bees, thus affecting their health. The purpose of this study was to determine the physiological and chemical changes caused by this fungicide in honey bee workers (Apis mellifera L). Workers of this honey bee at 1, 8, and 21 days old were treated with 125, 167, and 250 mg/L concentrations of azoxystrobin for seven days; their survival rates, activities of carboxylesterase (CarE), glutathione S-transferases (GSTs), cytochrome P450 enzyme (CYP450), catalase (CAT), and superoxide dismutase (SOD) enzymes, and the expression levels of immune (Aba, Api, Def1, and Hym) and nutrition genes (Ilp1, Ilp2, and Vg) were detected. Our findings revealed that azoxystrobin affected the survival of workers, particularly 1- and 21-day-old workers, who responded to azoxystrobin stress with increased activities of detoxification and protective enzymes, which might have physiological costs. Additionally, azoxystrobin affected the expression of immune and nutrition genes, with a decreased expression trend in 21-day-old workers compared to the 1- and 8-day-old workers, leading to reduced resistance to external stressors and increased mortality rates. These findings provide important insights into the adverse effects of azoxystrobin on workers of different ages and emphasize the potential risks of this chemical to colony stability and individual health. This study recommends an urgent ban on such a harmful fungicide being used for fungi control in agriculture, especially during plant flowering.
Keyword ：
Apis mellifera Apis mellifera azoxystrobin azoxystrobin enzyme activity enzyme activity gene expression gene expression survival survival
Cite：
Copy from the list or Export to your reference management。
| GB/T 7714 | Duan, Xinle , Tong, Wenlong , Tao, Bingfang et al. Azoxystrobin-Induced Physiological and Biochemical Alterations in Apis mellifera Workers of Different Ages [J]. | INSECTS , 2025 , 16 (5) . |
| MLA | Duan, Xinle et al. "Azoxystrobin-Induced Physiological and Biochemical Alterations in Apis mellifera Workers of Different Ages" . | INSECTS 16 . 5 (2025) . |
| APA | Duan, Xinle , Tong, Wenlong , Tao, Bingfang , Yao, Huanjing , Xiong, Manqiong , Liu, Huiping et al. Azoxystrobin-Induced Physiological and Biochemical Alterations in Apis mellifera Workers of Different Ages . | INSECTS , 2025 , 16 (5) . |
| Export to | NoteExpress RIS BibTex |
Version ：
Abstract ：
Vespa velutina (V. velutina) is a globally widespread predator of honeybees, posing a serious threat to them. Apis cerana (A. cerana) has acquired the ability to partially thwart the predation of the wasps, but the molecular mechanism by which it responds to V. velutina attacks is unknown. Here, both transcriptomic and metabolomic analyses were integrated to decipher what happened in the brain tissues of A. cerana exposed to V. velutina attacks. Transcriptomic analysis revealed 296 differentially expressed genes (DEGs) that were significantly enriched in neural signaling pathways (particularly serotonin and dopamine transmission), the cAMP signaling pathway, and energy metabolism. Metabolomic results showed that V. velutina attacks affected 38 metabolic pathways involving 86 differentially expressed metabolites (DEMs), primarily including amino acids, nucleotides, and carbohydrates. Several metabolites, such as isocitrate, xanthine, and adenosine, were upregulated in response to the hornet presence. Combined pathway analysis indicated an upregulation of the purine and tyrosine metabolism pathways, highlighting their critical role in A. cerana against invasive hornets. The results of this study will help to understand the molecular mechanisms of A. cerana in response to V. velutina attacks and provide valuable insights for enhancing collective defensive behavior against V. velutina in honeybees.
Keyword ：
Apis cerana Apis cerana defensive behavior defensive behavior honeybees honeybees metabolism metabolism transcriptome analysis transcriptome analysis
Cite：
Copy from the list or Export to your reference management。
| GB/T 7714 | Chen, Yijie , Xu, Xueling , Li, Yingjiao et al. Integrated Transcriptomic and Metabolomic Insights into "I See You" (ISY) Defensive Behavior in Apis cerana Against Vespa velutina [J]. | INSECTS , 2025 , 16 (10) . |
| MLA | Chen, Yijie et al. "Integrated Transcriptomic and Metabolomic Insights into "I See You" (ISY) Defensive Behavior in Apis cerana Against Vespa velutina" . | INSECTS 16 . 10 (2025) . |
| APA | Chen, Yijie , Xu, Xueling , Li, Yingjiao , Ji, Ning , Ruan, Yiwei , Yang, Mei et al. Integrated Transcriptomic and Metabolomic Insights into "I See You" (ISY) Defensive Behavior in Apis cerana Against Vespa velutina . | INSECTS , 2025 , 16 (10) . |
| Export to | NoteExpress RIS BibTex |
Version ：
Abstract ：
为探究啶氧菌酯对意大利蜜蜂Apis mellifera ligustica幼虫生长发育和免疫应答的影响，使用田间推荐浓度（113、150、225和281 mg/L）的啶氧菌酯连续饲喂意大利蜜蜂工蜂幼虫，记录处理后工蜂幼虫的存活率、化蛹率、蛹重、羽化率和羽化初生重，并测量幼虫体内发育相关基因、营养相关基因及免疫相关基因表达情况。结果显示，不同浓度啶氧菌酯对意大利蜜蜂工蜂幼虫存活率、蛹重和羽化初生重均有显著影响，且具有显著的浓度效应；150、225和281 mg/L啶氧菌酯处理后意大利蜜蜂工蜂幼虫的化蛹率和蛹的羽化率均较对照显著下降。不同浓度啶氧菌酯均可诱导幼虫体内蜕皮激素受体（ecdysone receptor,Ecr）基因、卵黄原蛋白（vitellogenin,Vg）基因、蜜蜂防卫素（defensin 1,Def1）基因和膜翅抗菌肽（hymenoptaecin,Hym）基因的表达量上调，且大部分与对照差异显著；不同浓度啶氧菌酯均可显著抑制幼虫体内胰岛素样肽（insulin-like peptide 1,ILP1）基因的表达量；281 mg/L啶氧菌酯分别显著抑制和显著增加幼虫体内蜜蜂抗菌肽（apidaecin,Api）基因和蜂蛾抗菌肽（abaecin,Aba）基因的表达量，225 mg/L和281 mg/L啶氧菌酯可诱导幼虫体内ILP2的表达量显著上调，显著抑制幼虫体内储存蛋白（hexamerin,Hex）基因Hex70b的表达量，其他浓度处理对幼虫体内Api、Aba、ILP2和Hex70b无显著影响。表明啶氧菌酯对意大利蜜蜂工蜂幼虫有慢性毒性，长时间暴露会扰乱幼虫的生长发育和免疫应答。在生产实践中应当考虑啶氧菌酯对意大利蜜蜂的潜在风险。
Keyword ：
免疫 免疫 啶氧菌酯 啶氧菌酯 幼虫 幼虫 意大利蜜蜂 意大利蜜蜂 生长发育 生长发育
Cite：
Copy from the list or Export to your reference management。
| GB/T 7714 | 段辛乐 , 王如意 , 熊曼琼 et al. 啶氧菌酯对意大利蜜蜂幼虫生长发育及免疫应答的影响 [J]. | 植物保护学报 , 2024 , 51 (02) : 432-441 . |
| MLA | 段辛乐 et al. "啶氧菌酯对意大利蜜蜂幼虫生长发育及免疫应答的影响" . | 植物保护学报 51 . 02 (2024) : 432-441 . |
| APA | 段辛乐 , 王如意 , 熊曼琼 , 王梨竹 , 覃淦 , 黄少康 et al. 啶氧菌酯对意大利蜜蜂幼虫生长发育及免疫应答的影响 . | 植物保护学报 , 2024 , 51 (02) , 432-441 . |
| Export to | NoteExpress RIS BibTex |
Version ：
Abstract ：
Honeybee larvae and pupae form the cornerstone of colony survival, development, and reproduction. Azoxystrobin is an effective strobilurin fungicide that is applied during the flowering stage for controlling plant pathogens. The contaminated nectar and pollen resulting from its application are collected by forager bees and impact the health of honeybee larvae and pupae. The current study evaluated the survival, development, and physiological effects of azoxystrobin exposure on the larvae and pupae of Apis mellifera worker bees. The field-recommended concentrations of azoxystrobin were found to suppress the survival indices and lifespan in the larval as well as pupal stages; moreover, the rates of the survival and pupation of larvae as well as the body weights of the pupae and newly-emerged adult bees were significantly reduced upon long-term exposure to azoxystrobin. In addition, azoxystrobin ingestion induced changes in the expression of genes critical for the development, immunity, and nutrient metabolism of larvae and pupae, although the expression profile of these genes differed between the larval and pupal stages. Results indicated the chronic toxicity of azoxystrobin on the growth and development of honeybee larvae and pupae, which would affect their sensitivity to pathogens and other external stresses during the development stage and the study will provide vital information regarding the pollination safety and rational use of pesticides.
Keyword ：
Apis mellifera Apis mellifera azoxystrobin azoxystrobin chronic toxicity chronic toxicity development development gene expression gene expression
Cite：
Copy from the list or Export to your reference management。
| GB/T 7714 | Duan, Xinle , Yao, Huanjing , Tong, Wenlong et al. Azoxystrobin Exposure Impacts on Development Status and Physiological Responses of Worker Bees (Apis mellifera L.) from Larval to Pupal Stages [J]. | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES , 2024 , 25 (21) . |
| MLA | Duan, Xinle et al. "Azoxystrobin Exposure Impacts on Development Status and Physiological Responses of Worker Bees (Apis mellifera L.) from Larval to Pupal Stages" . | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 25 . 21 (2024) . |
| APA | Duan, Xinle , Yao, Huanjing , Tong, Wenlong , Xiong, Manqiong , Huang, Shaokang , Li, Jianghong . Azoxystrobin Exposure Impacts on Development Status and Physiological Responses of Worker Bees (Apis mellifera L.) from Larval to Pupal Stages . | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES , 2024 , 25 (21) . |
| Export to | NoteExpress RIS BibTex |
Version ：
Abstract ：
Varroa destructor injects a salivary secretion into honeybees during their feeding process. The salivary secretion plays a vital role in mite-bee interactions and is the main cause of honeybee illness. To determine the biological function of cystatin-L2-like, one of the components of V. destructor salivary secretion, its gene expression in mites during the reproductive phase and dispersal phase was quantified using RT-qPCR, respectively. Moreover, the E. coli-expressed and -purified cystatin was injected into the white-eyed honeybee pupae, and its effects on the survival, the weight of the newly emerged bee, and the transcriptome were determined. The results showed that cystatin was significantly upregulated in mites during the reproductive phase. Cystatin significantly shortened the lifespan of pupae and decreased the weight of the newly emerged bees. Transcriptome sequencing showed that cystatin upregulated 1496 genes and downregulated 1483 genes in pupae. These genes were mainly enriched in ATP synthesis, the mitochondrial respiratory chain, and cuticle structure and function. Cystatin comprehensively downregulated the metabolism of carbohydrates, fatty acids, and amino acids, and energy production in the pupae. The downregulation of metabolic activity could save more nutrients and energy for V. destructor, helping it to maximize its reproduction potential, implying that the mite could manipulate the metabolism of host bees through the injected salivary secretion. The results provide new insights into mite-bee interactions, providing a basis for related studies and applications.
Keyword ：
honeybee pupae honeybee pupae manipulate manipulate metabolism metabolism salivary cystatin salivary cystatin Varroa destructor Varroa destructor
Cite：
Copy from the list or Export to your reference management。
| GB/T 7714 | Zhou, He , Duan, Xinle , Sun, Chaoxia et al. Salivary Cystatin-L2-like of Varroa destructor Causes Lower Metabolism Activity and Abnormal Development in Apis mellifera Pupae [J]. | ANIMALS , 2023 , 13 (23) . |
| MLA | Zhou, He et al. "Salivary Cystatin-L2-like of Varroa destructor Causes Lower Metabolism Activity and Abnormal Development in Apis mellifera Pupae" . | ANIMALS 13 . 23 (2023) . |
| APA | Zhou, He , Duan, Xinle , Sun, Chaoxia , Huang, Hongji , Yang, Mei , Huang, Shaokang et al. Salivary Cystatin-L2-like of Varroa destructor Causes Lower Metabolism Activity and Abnormal Development in Apis mellifera Pupae . | ANIMALS , 2023 , 13 (23) . |
| Export to | NoteExpress RIS BibTex |
Version ：
Abstract ：
The social division of labor within the honeybee colony is closely related to the age of the bees, and the age structure is essential to the development and survival of the colony. Differences in tolerance to pesticides and other external stresses among worker bees of different ages may be related to their social division of labor and corresponding physiological states. Pyraclostrobin was widely used to control the fungal diseases of nectar and pollen plants, though it was not friend to honey bees and other pollinators. This work aimed to determine the effects of field recommended concentrations of pyraclostrobin on the activities of protective and detoxifying enzymes, on the expression of genes involved in nutrient metabolism, and immune response in worker bees of different ages determined to investigate the physiological and biochemical differences in sensitivity to pyraclostrobin among different age of worker bees. The result demonstrates that the tolerance of adult worker bees to pyraclostrobin was negatively correlated with their age, and the significantly reduced survival rate of forager bees (21 day-old) with continued fungicide exposure. The activities of protective enzymes (CAT and SOD) and detoxifying enzymes (CarE, GSTs and CYP450) in different ages of adult worker bees were significantly altered, indicating the physiological response and the regulatory capacity of worker bees of different ages to fungicide stress was variation. Compared with 1 and 8 day-old worker bees, the expression of nutrient-related genes (ilp1 and ilp2) and immunity-related genes (apidaecin and defensin1) in forager bees (21 day-old) was gradually downregulated with increasing pyraclostrobin concentrations. Moreover, the expression of vitellogenin and hymenoptaecin in forager bees (21 day-old) was also decreased in high concentration treatment groups (250 and 313 mg/L). The present study confirmed the findings of the chronic toxicity of pyraclostrobin on the physiology and biochemistry of worker bees of different ages, especially to forager bees (21 day-old). These results would provide important physiological and biochemical insight for better understanding the potential risks of pyraclostrobin on honeybees and other non-target pollinators.
Keyword ：
Apis mellifera L. Apis mellifera L. Detoxification enzyme Detoxification enzyme Immunity response Immunity response Nutrition Nutrition Protection enzyme Protection enzyme Pyraclostrobin Pyraclostrobin
Cite：
Copy from the list or Export to your reference management。
| GB/T 7714 | Duan, Xinle , Wang, Lizhu , Wang, Ruyi et al. Variation in the physiological response of adult worker bees of different ages (Apis mellifera L.) to pyraclostrobin stress [J]. | ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY , 2023 , 269 . |
| MLA | Duan, Xinle et al. "Variation in the physiological response of adult worker bees of different ages (Apis mellifera L.) to pyraclostrobin stress" . | ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY 269 (2023) . |
| APA | Duan, Xinle , Wang, Lizhu , Wang, Ruyi , Xiong, Manqiong , Qin, Gan , Huang, Shaokang et al. Variation in the physiological response of adult worker bees of different ages (Apis mellifera L.) to pyraclostrobin stress . | ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY , 2023 , 269 . |
| Export to | NoteExpress RIS BibTex |
Version ：
Abstract ：
The strobilurin fungicide pyraclostrobin is widely used to prevent and control the fungal diseases of various nectar and pollen plants. Honeybees also directly or indirectly contact this fungicide with a long-term exposure period. However, the effects of pyraclostrobin on the development and physiology of Apis mellifera larvae and pupae during continuous exposure have been rarely known. To investigate the effects of field-realistic concentrations of pyraclostrobin on honeybee survival and development, the 2-day-old larvae were continuously fed with different pyraclostrobin solutions (100 mg/L and 83.3 mg/L), and the expression of development-, nutrient-, and immune-related genes in larvae and pupae were examined. The results showed that two field-realistic concentrations of pyraclostrobin (100 and 83.3 mg/L) significantly decreased the survival and capped rate of larvae, the weight of pupae and newly emerged adults, and such decrease was a positive correlation to the treatment concentrations. qPCR results showed that pyraclostrobin could induce the expression of Usp, ILP2, Vg, Defensin1, and Hymenoptaecin, decrease the expression of Hex100, Apidaecin, and Abaecin in larvae, could increase the expression of Ecr, Usp, Hex70b, Vg, Apidaecin, and Hymenoptaecin, and decreased the expression of ILP1, Hex100 and Defensin1in pupae. These results reflect pyraclostrobin could decrease nutrient metabolism, immune competence and seriously affect the development of honeybees. It should be used cautiously in agricultural practices, especially in the process of bee pollination.
Keyword ：
Apis mellifera Apis mellifera gene expression gene expression larvae larvae pupae pupae pyraclostrobin pyraclostrobin
Cite：
Copy from the list or Export to your reference management。
| GB/T 7714 | Xiong, Manqiong , Qin, Gan , Wang, Lizhu et al. Field recommended concentrations of pyraclostrobin exposure disturb the development and immune response of worker bees (Apis mellifera L.) larvae and pupae [J]. | FRONTIERS IN PHYSIOLOGY , 2023 , 14 . |
| MLA | Xiong, Manqiong et al. "Field recommended concentrations of pyraclostrobin exposure disturb the development and immune response of worker bees (Apis mellifera L.) larvae and pupae" . | FRONTIERS IN PHYSIOLOGY 14 (2023) . |
| APA | Xiong, Manqiong , Qin, Gan , Wang, Lizhu , Wang, Ruyi , Zhou, Ruiqi , Luo, Xiaotian et al. Field recommended concentrations of pyraclostrobin exposure disturb the development and immune response of worker bees (Apis mellifera L.) larvae and pupae . | FRONTIERS IN PHYSIOLOGY , 2023 , 14 . |
| Export to | NoteExpress RIS BibTex |
Version ：
Export
| Results： |
Selected to |
| Format： |
