Query：
学者姓名：马树杰
Refining：
Year
Type
Indexed by
Source
Complex
Co-Author
Language
Clean All
Abstract ：
H3N3 avian influenza viruses (AIVs) are less prevalent in poultry than H3N8 viruses. However, although relatively rare, reassortant H3N3 viruses have been known to appear in both domestic poultry and wild birds. In this study, we isolated the H3N3 virus in chickens sourced from a live poultry market in China. A comprehensive genomic analysis revealed that the virus possessed a single basic amino acid in the cleavage site of the hemagglutinin (HA) gene. Phylogenetic analysis indicated that eight genes in the H3N3 virus belong to the Eurasian lineage. Specifically, the HA and NA genes were clustered with H3N2 and H11N3, respectively, while the internal genes were closely related to the H3N8 and H9N2 viruses. Furthermore, the H3N3 virus exhibited high and moderate stability in thermal and acidic conditions and efficient replication capabilities in mammalian cells. The H3N3 virus demonstrated that it could infect and replicate in the upper and lower respiratory tract of BALB/c mice without prior adaptation, triggering hemagglutination inhibition (HI) antibody titres ranging from 80 to 160; notably, the H3N3 virus replicated vigorously within the chicken respiratory and digestive tracts. The virus also transmitted efficiently and swiftly among chickens through direct contact, leading to higher levels of HI antibodies in both the inoculated and contact birds. These findings suggest that the H3N3 virus may be a novel reassortant originating from viruses circulating in domestic poultry, thus demonstrating an increased pathogenicity and transmissibility in chickens. Our study determines that H3N3 AIV potentially threatens the poultry industry and public health, highlighting the importance of active surveillance of AIVs.
Keyword ：
avian influenza virus avian influenza virus evolution evolution pathogenicity pathogenicity transmissibility transmissibility Zoonoses Zoonoses
Cite：
Copy from the list or Export to your reference management。
| GB/T 7714 | Zhang, Chunping , Zhao, Conghui , Huang, Jiacheng et al. Emergence of a novel reassortant H3N3 avian influenza virus with enhanced pathogenicity and transmissibility in chickens in China [J]. | VETERINARY RESEARCH , 2025 , 56 (1) . |
| MLA | Zhang, Chunping et al. "Emergence of a novel reassortant H3N3 avian influenza virus with enhanced pathogenicity and transmissibility in chickens in China" . | VETERINARY RESEARCH 56 . 1 (2025) . |
| APA | Zhang, Chunping , Zhao, Conghui , Huang, Jiacheng , Wang, Yang , Jiang, Bo , Zheng, Hangyu et al. Emergence of a novel reassortant H3N3 avian influenza virus with enhanced pathogenicity and transmissibility in chickens in China . | VETERINARY RESEARCH , 2025 , 56 (1) . |
| Export to | NoteExpress RIS BibTex |
Version ：
Abstract ：
为了解H6N8亚型禽流感病毒（AIV）的分布特征，本研究统计分析了NCBI和GISAID数据库中400株H6N8亚型AIV的宿主、分离年份和地理分布特征，结果显示，H6N8亚型AIV在全球范围内持续流行，主要在野鸟和家禽中分离到，中国南方分布较多。为进一步探究福建省H6N8亚型AIV的遗传进化特征，本研究于2023年在活禽市场采集285份家禽泄殖腔拭子样品，经接种鸡胚，37℃培养48 h后分离到1株AIV，经RT-PCR和测序分析鉴定为H6N8亚型AIV，并对其进行了全基因组测序、遗传进化分析和关键位点氨基酸序列分析。序列分析结果显示，该病毒HA蛋白裂解位点氨基酸序列为PQIETR↓GLF，无连续碱基氨基酸，符合低致病性AIV（LPAIV）的分子特征。HA蛋白
Keyword ：
H6N8亚型AIV H6N8亚型AIV 三间分布 三间分布 关键氨基酸 关键氨基酸 禽流感病毒 禽流感病毒 遗传进化 遗传进化
Cite：
Copy from the list or Export to your reference management。
| GB/T 7714 | 黄佳承 , 彭燕妮 , 郑航煜 et al. 一株重组H6N8亚型禽流感病毒的遗传进化分析 [J]. | 中国预防兽医学报 , 2025 , 47 (05) : 443-451 . |
| MLA | 黄佳承 et al. "一株重组H6N8亚型禽流感病毒的遗传进化分析" . | 中国预防兽医学报 47 . 05 (2025) : 443-451 . |
| APA | 黄佳承 , 彭燕妮 , 郑航煜 , 庄明智 , 张春萍 , 王洋 et al. 一株重组H6N8亚型禽流感病毒的遗传进化分析 . | 中国预防兽医学报 , 2025 , 47 (05) , 443-451 . |
| Export to | NoteExpress RIS BibTex |
Version ：
Abstract ：
本发明公开了一种宿主蛋白SNAPIN，经体外研究发现SNAPIN蛋白具有拮抗流感病毒复制的作用，并且SNAPIN蛋白具有增强干扰素和干扰素诱导基因表达的作用，在抗流感病毒药物制备或免疫增强剂制备中具有应用价值。
Cite：
Copy from the list or Export to your reference management。
| GB/T 7714 | 马树杰 , 赵聪慧 , 张晓璇 . SNAPIN蛋白及其编码基因在抗流感病毒中的应用 : CN202410023219.7[P]. | 2024-01-08 . |
| MLA | 马树杰 et al. "SNAPIN蛋白及其编码基因在抗流感病毒中的应用" : CN202410023219.7. | 2024-01-08 . |
| APA | 马树杰 , 赵聪慧 , 张晓璇 . SNAPIN蛋白及其编码基因在抗流感病毒中的应用 : CN202410023219.7. | 2024-01-08 . |
| Export to | NoteExpress RIS BibTex |
Version ：
Abstract ：
Influenza A viruses (IAVs) are highly contagious pathogens that cause zoonotic disease with limited availability of antiviral therapies, presenting ongoing challenges to both public health and the livestock industry. Unveiling host proteins that are crucial to the IAV life cycle can help clarify mechanisms of viral replication and identify potential targets for developing alternative host-directed therapies. Using a four-dimensional (4D), label-free methodology coupled with bioinformatics analysis, we analyzed the expression patterns of cellular proteins that changed following H9N2 virus infection. Compared to the control group, the H9N2 infected group displayed 732 differentially expressed proteins (DEPs), with 298 proteins showing upregulation and 434 proteins showing downregulation. Gene Ontology (GO) functional analysis showed that DEPs were catalog in 11 biological processes, three cellular components, and eight molecular functions. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis revealed that DEPs were involved in processes including cytokine signaling pathways induced by virus infection and protein digestion and absorption. Proteins including TP53, DDX58, and STAT3 were among the top hub proteins in the protein-protein interaction (PPI) analysis, suggesting that these signaling cascades could be essential for the propagation of IAVs. Furthermore, the host protein SNAPIN was chosen to ascertain the accuracy of expression changes identified through a proteomic analysis. The results indicated that SNAPIN was downregulated following infection with IAVs both in vitro and in vivo, which is consistent with the proteomics results, suggesting that SNAPIN may serve as a key regulatory factor in the viral life cycle of IAVs. Our research delineates an extensive interaction map of IAV infection within the A549 cells, facilitating the discovery of pivotal proteins that contribute to the virus's propagation, potentially offering target candidates to screen for antiviral therapeutics.
Keyword ：
avian influenza virus avian influenza virus bioinformatics analysis bioinformatics analysis mass spectrometry mass spectrometry proteomic analysis proteomic analysis SNAPIN SNAPIN
Cite：
Copy from the list or Export to your reference management。
| GB/T 7714 | Zhao, Conghui , Zhang, Xiaoxuan , Wang, Huanhuan et al. Proteomic Analysis of Differentially Expressed Proteins in A549 Cells Infected with H9N2 Avian Influenza Virus [J]. | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES , 2025 , 26 (2) . |
| MLA | Zhao, Conghui et al. "Proteomic Analysis of Differentially Expressed Proteins in A549 Cells Infected with H9N2 Avian Influenza Virus" . | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 26 . 2 (2025) . |
| APA | Zhao, Conghui , Zhang, Xiaoxuan , Wang, Huanhuan , Qiang, Haoxi , Liu, Sha , Zhang, Chunping et al. Proteomic Analysis of Differentially Expressed Proteins in A549 Cells Infected with H9N2 Avian Influenza Virus . | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES , 2025 , 26 (2) . |
| Export to | NoteExpress RIS BibTex |
Version ：
Abstract ：
The H12 subtypes of avian influenza viruses (AIVs) are globally prevalent in wild birds, occasionally spilling over into poultry. In this study, we isolated an H12N8 virus from ducks in a live poultry market. Full genomic analysis revealed that the virus bears a single basic amino acid in the cleavage site of the hemagglutinin gene. Phylogenetic analysis revealed that the eight gene segments of the H12N8 virus belong to the Eurasian lineage and the HA gene was clustered with wild bird-originated H12 viruses, with its NP gene showing the highest nucleotide similarity to 2013-like H7N9 viruses. The H12N8 virus replicated effectively in both mammalian and avian cells without prior adaptation. Moreover, the H12N8 virus could infect and replicate in the upper respiratory tract of BALB/c mice without prior adaptation. The H12N8 virus replicated and transmitted inefficiently in both ducks and chickens and hardly triggered high hemagglutination inhibition (HI) antibody titers in the inoculated and contact animals. These results suggest that the wild bird-origin H12N8 virus has reassorted with viruses circulating in domestic poultry, but it inefficiently replicates and transmits in avian hosts. Our findings demonstrate that H12N8 AIV has emerged in domestic poultry, emphasizing the importance of active surveillance of AIVs in both wild and domestic birds.
Keyword ：
avian influenza virus avian influenza virus evolution evolution pathogenicity pathogenicity transmissibility transmissibility
Cite：
Copy from the list or Export to your reference management。
| GB/T 7714 | Zhao, Conghui , Huang, Jiacheng , Zhang, Chunping et al. Characteristics of the First Domestic Duck-Origin H12N8 Avian Influenza Virus in China [J]. | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES , 2025 , 26 (6) . |
| MLA | Zhao, Conghui et al. "Characteristics of the First Domestic Duck-Origin H12N8 Avian Influenza Virus in China" . | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 26 . 6 (2025) . |
| APA | Zhao, Conghui , Huang, Jiacheng , Zhang, Chunping , Wang, Yang , Zhang, Xiaoxuan , Liu, Sha et al. Characteristics of the First Domestic Duck-Origin H12N8 Avian Influenza Virus in China . | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES , 2025 , 26 (6) . |
| Export to | NoteExpress RIS BibTex |
Version ：
Abstract ：
旨在构建稳定过表达环状RNA LAMP3(circLAMP3)的C57/B6-L和A549细胞系，为后续深入研究circLAMP3的生物学功能奠定基础。分别提取C57/B6-L和A549细胞总RNA,反转录后扩增circLAMP3全长序列，连接到pLC5-ciR载体上，得到pLC5-Mouse-circLAMP3和pLC5-Human-circLAMP3重组质粒。利用瞬时转染方法在HEK293T细胞上包装慢病毒，将慢病毒分别感染C57/B6-L和A549细胞，经嘌呤霉素筛选得到稳定表达circLAMP3的细胞系。利用荧光显微镜、PCR扩增、荧光定量PCR(qPCR)和Sanger测序对构建的细胞系过表达circLAMP3效果进行验证。结果表明，pLC5-Mouse-circLAMP3和pLC5-Human-circLAMP3过表达质粒构建成功，荧光显微镜下可见构建的C57/B6-L和A549细胞系表达强烈绿色荧光。PCR和qPCR结果可见过表达细胞系circLAMP3表达显著增强，Sanger测序结果表明circLAMP3环化位点正确。本研究成功构建了过表达circLAMP3的C57/B6-L和A549细胞系，为进一步探索circLAMP3在流感病毒复制中的生物学功能提供了生物材料。
Keyword ：
circLAMP3 circLAMP3 环状RNA 环状RNA 细胞系 细胞系 载体构建 载体构建
Cite：
Copy from the list or Export to your reference management。
| GB/T 7714 | 陈福再 , 赵聪慧 , 张晓璇 et al. 稳定表达circLAMP3的C57/B6-L和A549细胞系的构建 [J]. | 中国兽医学报 , 2024 , 44 (09) : 2010-2016 . |
| MLA | 陈福再 et al. "稳定表达circLAMP3的C57/B6-L和A549细胞系的构建" . | 中国兽医学报 44 . 09 (2024) : 2010-2016 . |
| APA | 陈福再 , 赵聪慧 , 张晓璇 , 张春萍 , 黄佳承 , 陈吉龙 et al. 稳定表达circLAMP3的C57/B6-L和A549细胞系的构建 . | 中国兽医学报 , 2024 , 44 (09) , 2010-2016 . |
| Export to | NoteExpress RIS BibTex |
Version ：
Abstract ：
本发明公开了一种宿主蛋白SNAPIN，经体外研究发现SNAPIN蛋白具有拮抗流感病毒复制的作用，并且SNAPIN蛋白具有增强干扰素和干扰素诱导基因表达的作用，在抗流感病毒药物制备或免疫增强剂制备中具有应用价值。
Cite：
Copy from the list or Export to your reference management。
| GB/T 7714 | 赵聪慧 , 张晓璇 , 马树杰 . SNAPIN蛋白及其编码基因在抗流感病毒中的应用 : 2024100232197[P]. | 2024-01-08 . |
| MLA | 赵聪慧 et al. "SNAPIN蛋白及其编码基因在抗流感病毒中的应用" : 2024100232197. | 2024-01-08 . |
| APA | 赵聪慧 , 张晓璇 , 马树杰 . SNAPIN蛋白及其编码基因在抗流感病毒中的应用 : 2024100232197. | 2024-01-08 . |
| Export to | NoteExpress RIS BibTex |
Version ：
Abstract ：
【目的】猪瘟病毒(classical swine fever virus,CSFV)感染猪引起出血综合征和免疫抑制，是一种高度传染性的猪病。本研究以CSFV为研究对象，探究CSFV抑制宿主天然免疫的机制及其在免疫抑制条件下对甲型流感病毒(influenza A virus,IAV)感染的潜在作用。【方法】首先，为探究CSFV对宿主天然免疫的影响，利用反转录PCR (reverse transcription-PCR,RT-PCR)、荧光定量PCR (reverse transcription-quantitative PCR,RT-q PCR)和Western blotting技术检测PK-15细胞感染CSFV后对Poly(I:C)诱导干扰素(interferons,IFNs)、干扰素诱导基因(IFN-stimulated genes,ISGs)和信号传导子和转录活化子1 (signal transducer and activator of transcription 1,STAT1)磷酸化的影响。其次，利用过表达CSFV蛋白的细胞系筛选并确定抑制天然免疫的关键蛋白。最后，在过表达CSFV N~(pro)蛋白的细胞系上，利用RT-PCR、RT-q PCR、Western blotting和病毒噬斑实验技术研究N~(pro)蛋白对天然免疫和IAV感染的影响。【结果】CSFV可抑制poly(I:C)诱导的Ⅰ型和Ⅲ型IFN的表达。CSFV N~(pro)蛋白在体外直接抑制STAT1的磷酸化，引起寡腺苷酸合成酶样蛋白(OASL)、2′,5′-寡腺苷酸合成酶1 (OAS1)、干扰素诱导跨膜蛋白3 (IFITM3)和干扰素刺激基因15 (ISG15)表达下调。重要的是，CSFV N~(pro)蛋白可抑制IAV诱导的Ⅰ型和Ⅲ型IFN的表达，并导致STAT1的磷酸化和OASL、OAS1、IFITM3和ISG15的表达显著下降，进而显著促进IAV在过表达N~(pro)蛋白的PK-15细胞中复制。【结论】猪瘟病毒的N~(pro)蛋白能够拮抗由poly(I:C)和IAV所激活的天然免疫应答，表明N~(pro)蛋白可抑制RIG-I依赖的信号通路并可促进IAV在表达N~(pro)蛋白的PK15细胞中复制。
Keyword ：
N~(pro) N~(pro) 信号传导子和转录活化子1(STAT1) 信号传导子和转录活化子1(STAT1) 天然免疫 天然免疫 干扰素 干扰素 猪瘟病毒 猪瘟病毒 甲型流感病毒 甲型流感病毒
Cite：
Copy from the list or Export to your reference management。
| GB/T 7714 | 施文豪 , 蔡彬祥 , 杨彬偲 et al. Npro蛋白可抑制 poly(I:C)和流感病毒激活的天然免疫应答 [J]. | 微生物学报 , 2022 , 62 (12) : 4894-4917 . |
| MLA | 施文豪 et al. "Npro蛋白可抑制 poly(I:C)和流感病毒激活的天然免疫应答" . | 微生物学报 62 . 12 (2022) : 4894-4917 . |
| APA | 施文豪 , 蔡彬祥 , 杨彬偲 , 邱昊日 , 文法鑫 , 池晓娟 et al. Npro蛋白可抑制 poly(I:C)和流感病毒激活的天然免疫应答 . | 微生物学报 , 2022 , 62 (12) , 4894-4917 . |
| Export to | NoteExpress RIS BibTex |
Version ：
Abstract ：
<正>引言H9N2亚型禽流感病毒(avian influenza virus,AIV)不仅感染家禽,危害养殖业发展,而且该亚型病毒容易与其他亚型流感病毒发生基因重组,为新型重组AIV提供内部基因,在流感病毒的遗传演化中发挥重要作用,对公共卫生安全造成持续威胁[1]。例如2013年安徽和上海等地爆发的H7N9[2]以及江西爆发的H0N8[3]新型AIV,其内部基因均来自H9N2AIV。
Keyword ：
AIV AIV 流行病学调查 流行病学调查 福建省 福建省 禽流感病毒 禽流感病毒
Cite：
Copy from the list or Export to your reference management。
| GB/T 7714 | 马树杰 , 王辰川 , 李菁 et al. 福建省H9N2亚型禽流感病毒的流行病学调查 [C] //中国畜牧兽医学会动物传染病学分会第十九次全国学术研讨会 . 2021 . |
| MLA | 马树杰 et al. "福建省H9N2亚型禽流感病毒的流行病学调查" 中国畜牧兽医学会动物传染病学分会第十九次全国学术研讨会 . (2021) . |
| APA | 马树杰 , 王辰川 , 李菁 , 文法鑫 , 李欣欣 , 李训良 et al. 福建省H9N2亚型禽流感病毒的流行病学调查 中国畜牧兽医学会动物传染病学分会第十九次全国学术研讨会 . (2021) . |
| Export to | NoteExpress RIS BibTex |
Version ：
Abstract ：
Non-coding RNAs (ncRNAs) are extensively expressed in various cells and tissues, and studies have shown that ncRNAs play significant roles in cell regulation. However, in the past few decades, the knowledge of ncRNAs has been increased dramatically due to their transcriptional ability and multiple regulatory functions. Typically, regulatory ncRNAs include long ncRNAs (lncRNAs), miRNAs, piRNAs, Y RNAs, vault RNAs, and circular RNAs (circRNAs), etc. Previous studies have revealed that various ncRNAs are involved in the host responses to virus infection and play critical roles in the regulation of host-virus interactions. In this review, we discuss the conceptual framework and biological regulations of ncRNAs to elucidate their functions in response to viral infection, especially influenza A virus (IAV) infection. In addition, we summarize the ncRNAs that are associated with innate immunity and involvement of interferons and their stimulated genes (ISGs) during IAV infection.
Keyword ：
infection infection influenza A virus influenza A virus innate immunity innate immunity interferons interferons non-coding RNAs non-coding RNAs
Cite：
Copy from the list or Export to your reference management。
| GB/T 7714 | Sajjad, Nelam , Wang, Song , Liu, Ping et al. Functional Roles of Non-coding RNAs in the Interaction Between Host and Influenza A Virus [J]. | FRONTIERS IN MICROBIOLOGY , 2021 , 12 . |
| MLA | Sajjad, Nelam et al. "Functional Roles of Non-coding RNAs in the Interaction Between Host and Influenza A Virus" . | FRONTIERS IN MICROBIOLOGY 12 (2021) . |
| APA | Sajjad, Nelam , Wang, Song , Liu, Ping , Chen, Ji-Long , Chi, Xiaojuan , Liu, Shasha et al. Functional Roles of Non-coding RNAs in the Interaction Between Host and Influenza A Virus . | FRONTIERS IN MICROBIOLOGY , 2021 , 12 . |
| Export to | NoteExpress RIS BibTex |
Version ：
Export
| Results： |
Selected to |
| Format： |
