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学者姓名：廖吕燕
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Abstract ：
Mycoplasma synoviae (MS) infection can cause severe inflammatory responses in avian species, evading immune recognition, leading to chronic infection and immune dysfunction. Although the pathogenic mechanisms and immune evasion strategies of MS have not been fully elucidated, the crucial role of the spleen in immune responses cannot be overlooked. Building on preliminary findings related to the tissue damage and inflammatory responses in chicken spleens caused by MS infection, this study further utilizes transcriptomic and proteomic technologies to thoroughly investigate the comprehensive effects of MS infection on the chicken spleen. We detected 946 differentially expressed genes (DEGs) and 305 differentially expressed proteins (DEPs) during MS infection, including 771 up-regulated and 175 down-regulated DEGs, along with 145 up-regulated and 160 down-regulated DEPs. Gene Ontology (GO) analysis indicated that the DEGs/DEPs are enriched in processes related to immune activation, antioxidation/cell apoptosis, inflammation, and endoplasmic reticulum protein processing. KEGG pathway enrichment analysis revealed immune-related pathways closely associated with MS infection, particularly in endoplasmic reticulum protein processing, cytokine-cytokine receptor interaction, and the myosin light chain kinase (MAPK) signalling pathway. Suggesting that the activation and interaction of these pathways may play a critical role in the immune response during MS infection. Overall, this study, for the first time, employs RNA-Seq and TMT quantitative proteomics to comprehensively analyse the interaction mechanisms between MS and chicken spleen, enhancing our understanding of the complexity of the immune mechanisms induced by MS. This provides valuable insights for future research and intervention strategies targeting MS infection.
Keyword ：
immune responses immune responses Mycoplasma synoviae Mycoplasma synoviae proteomic analysis proteomic analysis spleen spleen transcriptomic analysis transcriptomic analysis
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| GB/T 7714 | Zhong, Lemiao , Wu, Chunlin , Liao, Lvyan et al. Comparative omics analysis of the impact of Mycoplasma synoviae infection on the immune mechanisms of poultry spleen [J]. | VIRULENCE , 2025 , 16 (1) . |
| MLA | Zhong, Lemiao et al. "Comparative omics analysis of the impact of Mycoplasma synoviae infection on the immune mechanisms of poultry spleen" . | VIRULENCE 16 . 1 (2025) . |
| APA | Zhong, Lemiao , Wu, Chunlin , Liao, Lvyan , Li, Jian , Wu, Yijian . Comparative omics analysis of the impact of Mycoplasma synoviae infection on the immune mechanisms of poultry spleen . | VIRULENCE , 2025 , 16 (1) . |
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Abstract ：
Mycoplasma synovium (MS) is a prominent avian pathogen known to elicit robust inflammatory responses in birds while evading immune detection, often leading to chronic infection and immune compromise. The mechanisms underpinning MS-mediated splenic tissue damage in chickens, however, remain undefined. In our investigation with 7-day-old SPF chickens, we administered an MS-Y bacterial solution (200 mu l, 1 x 10(9) CCU/ml) through eye and nose droplets, collecting spleen samples on days 3, 6, and 12 post-infection. Comprehensive analyses utilizing histopathology, electron microscopy, TUNEL assay, qRT-PCR, and western blot were employed. Results demonstrated that MS-infection downregulated T-SOD, GSH-PX, and CAT, while concurrently elevating iNOS, NO, and MDA levels. Evidently, MS-induced oxidative stress compromised the spleen's antioxidant defences. Histological examinations pinpointed splenic damage characterized by lymphocyte reduction and increased inflammatory cell infiltration. Ultrastructural observations revealed clear apoptotic markers, including mitochondrial perturbations and nuclear anomalies. Importantly, MS induced significant spleen tissue apoptosis, as supported by TUNEL assay outputs and gene expression profiles associated with apoptosis. Concurrently, we observed upregulated expressions of mRNAs and proteins affiliated with the NF-kappa B/MAPK signalling cascade (p < 0.05). Collectively, our data elucidate that MS infection induces splenic apoptosis and oxidative disturbances, perturbs tissue integrity, and potentiates the NF-kappa B/MAPK-mediated inflammatory cascade.
Keyword ：
apoptosis apoptosis inflammation inflammation Mycoplasma synovium Mycoplasma synovium oxidative stress oxidative stress spleen spleen
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| GB/T 7714 | Zhong, Lemiao , Wu, Chunlin , Liao, Lvyan et al. Mycoplasma synoviae induce spleen tissue damage and inflammatory response of chicken through oxidative stress and apoptosis [J]. | VIRULENCE , 2024 , 15 (1) . |
| MLA | Zhong, Lemiao et al. "Mycoplasma synoviae induce spleen tissue damage and inflammatory response of chicken through oxidative stress and apoptosis" . | VIRULENCE 15 . 1 (2024) . |
| APA | Zhong, Lemiao , Wu, Chunlin , Liao, Lvyan , Wu, Yijian . Mycoplasma synoviae induce spleen tissue damage and inflammatory response of chicken through oxidative stress and apoptosis . | VIRULENCE , 2024 , 15 (1) . |
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本发明公开一种鸡滑液支原体间接ELISA检测试剂盒及检测方法。以氨基酸序列如SEQ ID NO.1所示的鸡滑液囊支原体抗原蛋白MS‑eLPD作为包被抗原构建鸡滑液支原体间接ELISA检测试剂盒及检测方法，用于检测鸡滑液支原体抗体水平，与鸡毒支原体阳性血清、鸡传染性支气管炎阳性血清、鸡传染性法氏囊病阳性血清、禽白血病阳性血清、禽网状内皮组织增生症阳性血清、鸡传染性鼻气管炎阳性血清无交叉反应，具有良好的特异性和重复性，为鸡滑液支原体的临床诊断、流行病学研究和免疫检测提供了有效的手段。
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| GB/T 7714 | 吴异健 , 钟乐苗 , 廖吕燕 . 一种鸡滑液支原体间接ELISA检测试剂盒及检测方法 : CN202310623656.8[P]. | 2023-05-30 . |
| MLA | 吴异健 et al. "一种鸡滑液支原体间接ELISA检测试剂盒及检测方法" : CN202310623656.8. | 2023-05-30 . |
| APA | 吴异健 , 钟乐苗 , 廖吕燕 . 一种鸡滑液支原体间接ELISA检测试剂盒及检测方法 : CN202310623656.8. | 2023-05-30 . |
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Muscovy duck reovirus (MDRV) infection induces serious immunosuppression and intestinal injury in Muscovy ducklings with a high morbidity and mortality, and Astragalus polysaccharide (APS) pretreatment could efficiently protect ducklings from MDRV infection, although the underlying immunoregulatory mechanisms remain unclear. Thus, the objective of this study was to investigate effects of APS on the intestinal mucosal immunity in MDRV-infected Muscovy ducklings. A total of 190 1-day-old healthy Muscovy ducklings were randomly assigned to 3 groups (n = 50): normal control group, APS pretreatment for MDRV-infected group, and cohabitation infection group, then pretreated with 0.6 g/L APS or only drinking water followed byMDRVcohabitation infection with the remaining 40 artificially infected ducklings, respectively. At the 2, 3, 4, 6, 9 and 15 d after cohabitation infection, the intestinal samples were prepared to measure intestinal parameters including villus length, villus length/crypt depth (V/C) ratio, and wall thickness, together with counts of intraepithelial lymphocyte (IEL) and goblet cell (GC) by hematoxylin-eosin staining. Meanwhile, ileal secretory IgA (sIgA) and duodenal cytokine levels of IL-4, IL-6, IL-15, tumor necrosis factor-alpha, and interferon gamma were detected by the ELISA and radioimmunoassay, respectively. The results showed that APS significantly improved intestinal injuries of villi length, V/C ratio, and wall thickness of the small intestine infected with MDRV, effectively inhibited the reduction of IEL and GC caused by MDRV infection, subsequently increased sIgA and all the cytokine secretions at most time points, suggesting that APS pretreatment can effectively stimulate mucosal immune function by improving intestinal morphology and repair MDRV caused injures of small intestinal mucosal immune barrier in infected ducklings. Our findings lay the foundation for further application of APS in prevention and treatment of MDRV infection.
Keyword ：
Astragalus polysaccharide Astragalus polysaccharide duckling duckling Muscovy duck reovirus Muscovy duck reovirus
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| GB/T 7714 | Liao, Lvyan , Li, Jian , Li, Jun et al. Effects of Astragalus polysaccharides on intestinal morphology and intestinal immune cells of Muscovy ducklings infected with Muscovy duck reovirus [J]. | POULTRY SCIENCE , 2021 , 100 (1) : 64-72 . |
| MLA | Liao, Lvyan et al. "Effects of Astragalus polysaccharides on intestinal morphology and intestinal immune cells of Muscovy ducklings infected with Muscovy duck reovirus" . | POULTRY SCIENCE 100 . 1 (2021) : 64-72 . |
| APA | Liao, Lvyan , Li, Jian , Li, Jun , Huang, Yifan , Wu, Yijian . Effects of Astragalus polysaccharides on intestinal morphology and intestinal immune cells of Muscovy ducklings infected with Muscovy duck reovirus . | POULTRY SCIENCE , 2021 , 100 (1) , 64-72 . |
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It has been shown that Muscovy duck reovirus (MDRV) infection causes severe intestinal barrier damage and intestinal mucosal immune suppression. The health and balance of gut microbes is essential for the progression of intestinal infectious diseases. To investigate the interaction of MDRV, intestinal bacteria with host intestinal innate immunity, an MDRV contact-infection model was established in this study. High-throughput sequencing technology was used to sequence 16S rDNA and transcripts in ileal samples from experimental Muscovy ducklings. Our results suggest that intestinal opportunistic pathogens such as Streptococcus and Corynebacterium proliferated massively in MDRV-infected Muscovy ducklings. The body initiates antiviral and antibacterial immunity and actively fights the infection of gut microbes. The synthesis of peptidoglycan, lipopolysaccharide, and flagellin by intestinal bacteria activates the Toll-like receptor signaling pathway resulting in increased secretion of IFN-beta, IL-1 beta, and IL-8. The RIG-I-like receptor signaling pathway is an important signaling pathway for the interaction between MDRV and the host. At the same time, we also observed that multiple genes in the JAK-STAT signaling pathway were significantly different. These genes are important targets for studying the immunosuppression caused by MDRV. In conclusion, we analyzed the interaction of MDRV, intestinal flora and host immune system during MDRV infection, which provides a basis for the further study on the mechanism of intestinal immunosuppression caused by MDRV.
Keyword ：
Gut microbiota Gut microbiota Innate immune Innate immune Muscovy duck reovirus Muscovy duck reovirus PICRUSt functional analysis PICRUSt functional analysis Transcriptome sequencing Transcriptome sequencing
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| GB/T 7714 | Zhu, Zheng , Liao, Lvyan , Su, Jiawen et al. Interactions of Muscovy duck reovirus, gut microbiota, and host innate immunity: Transcriptome and gut microbiota analysis [J]. | VETERINARY MICROBIOLOGY , 2021 , 264 . |
| MLA | Zhu, Zheng et al. "Interactions of Muscovy duck reovirus, gut microbiota, and host innate immunity: Transcriptome and gut microbiota analysis" . | VETERINARY MICROBIOLOGY 264 (2021) . |
| APA | Zhu, Zheng , Liao, Lvyan , Su, Jiawen , Liu, Zhenni , Pan, Shulei , Huang, Yifan et al. Interactions of Muscovy duck reovirus, gut microbiota, and host innate immunity: Transcriptome and gut microbiota analysis . | VETERINARY MICROBIOLOGY , 2021 , 264 . |
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To investigate the effects of Hericium erinaceus polysaccharide (HEP) on immunity in Muscovy duck reovirus (MDRV)-infected ducklings and explore its mechanism of action, an MDRV contact-infection model was established. Then, we investigated the influence of HEP on morphology of main immune organs in MDRV-infected ducklings by HE staining, while antioxidant capacity (T-AOC, MDA), serum protein levels (TP, ALB, GLO), complement levels (C-3, C-4) and antibody levels (IgA, IgM, IgG) were detected. Apoptotic indexes (apoptosisi rate and FAS-L) were also quantified by TUNEL method and immunohistochemical staining. Meanwhile, FADD and CytC (apoptosis-related genes), were tested by quantitative RT-PCR. Results showed that HEP could reduce the injuries of immune organs caused by MDRV. Additionally, HEP markedly diminished MDA (p < 0.01), while significantly increased T-AOC, TP, ALB, GLO, C-3, C-4, IgA, IgM and IgG (p < 0.01 or p < 0.05). Then, HEP shifted apoptosis time to an early MDRV-infected stage and reduced apoptosis at later MDRVinfected stage. This was associated with changes of FADD and CytC. Collectively, our data suggested that HEP could reduce the immunesuppression by many ways, such as decreasing organs' injuries, improving antioxidant capacity, serumproteins levels, antibody levels and complement levels, while diminish the apoptosis by lowering the FADD and CytC. (C) 2021 Elsevier B.V. All rights reserved.
Keyword ：
Apoptosis Apoptosis Hericium erinaceus polysaccharide Hericium erinaceus polysaccharide Immunity Immunity Muscovy duck reovirus Muscovy duck reovirus
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| GB/T 7714 | Liu, Zhenni , Liao, Lvyan , Chen, Qiang et al. Effects of Hericium erinaceus polysaccharide on immunity and apoptosis of the main immune organs in Muscovy duck reovirus-infected ducklings [J]. | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES , 2021 , 171 : 448-456 . |
| MLA | Liu, Zhenni et al. "Effects of Hericium erinaceus polysaccharide on immunity and apoptosis of the main immune organs in Muscovy duck reovirus-infected ducklings" . | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 171 (2021) : 448-456 . |
| APA | Liu, Zhenni , Liao, Lvyan , Chen, Qiang , Lin, Shaoqing , Luo, Yu , Qin, Tao et al. Effects of Hericium erinaceus polysaccharide on immunity and apoptosis of the main immune organs in Muscovy duck reovirus-infected ducklings . | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES , 2021 , 171 , 448-456 . |
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Abstract ：
To investigate the effects of Hericium erinaceus polysaccharide (HEP) on immunity in Muscovy duck reovirus (MDRV)-infected ducklings and explore its mechanism of action, an MDRV contact-infection model was established. Then, we investigated the influence of HEP on morphology of main immune organs in MDRV-infected ducklings by HE staining, while antioxidant capacity (T-AOC, MDA), serum protein levels (TP, ALB, GLO), complement levels (C-3, C-4) and antibody levels (IgA, IgM, IgG) were detected. Apoptotic indexes (apoptosisi rate and FAS-L) were also quantified by TUNEL method and immunohistochemical staining. Meanwhile, FADD and CytC (apoptosis-related genes), were tested by quantitative RT-PCR. Results showed that HEP could reduce the injuries of immune organs caused by MDRV. Additionally, HEP markedly diminished MDA (p < 0.01), while significantly increased T-AOC, TP, ALB, GLO, C-3, C-4, IgA, IgM and IgG (p < 0.01 or p < 0.05). Then, HEP shifted apoptosis time to an early MDRV-infected stage and reduced apoptosis at later MDRVinfected stage. This was associated with changes of FADD and CytC. Collectively, our data suggested that HEP could reduce the immunesuppression by many ways, such as decreasing organs' injuries, improving antioxidant capacity, serumproteins levels, antibody levels and complement levels, while diminish the apoptosis by lowering the FADD and CytC. (C) 2021 Elsevier B.V. All rights reserved.
Keyword ：
Apoptosis Apoptosis Hericium erinaceus polysaccharide Hericium erinaceus polysaccharide Immunity Immunity Muscovy duck reovirus Muscovy duck reovirus
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| GB/T 7714 | Liu, Zhenni , Liao, Lvyan , Chen, Qiang et al. Effects of Hericium erinaceus polysaccharide on immunity and apoptosis of the main immune organs in Muscovy duck reovirus-infected ducklings [J]. | International Journal of Biological Macromolecules: Structure, Function and Interactions , 2021 , 171 : 448-456 . |
| MLA | Liu, Zhenni et al. "Effects of Hericium erinaceus polysaccharide on immunity and apoptosis of the main immune organs in Muscovy duck reovirus-infected ducklings" . | International Journal of Biological Macromolecules: Structure, Function and Interactions 171 (2021) : 448-456 . |
| APA | Liu, Zhenni , Liao, Lvyan , Chen, Qiang , Lin, Shaoqing , Luo, Yu , Qin, Tao et al. Effects of Hericium erinaceus polysaccharide on immunity and apoptosis of the main immune organs in Muscovy duck reovirus-infected ducklings . | International Journal of Biological Macromolecules: Structure, Function and Interactions , 2021 , 171 , 448-456 . |
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《中兽医学》实验是动物医学专业的一门重要专业课。为了提高《中兽医学》实验课教学效果,针对传统实验教学过程存在的诸多不足,从教学内容、教学手段、方法和考核方式上进行课程教学改革。通过精心设计实验、丰富教学手段和改进教学方法,优化考核方式,充分发挥学生的积极性和主动性,增强学生分析问题和解决问题的能力,提高教学质量。
Keyword ：
中兽医学 中兽医学 实验教学 实验教学 教学改革 教学改革
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| GB/T 7714 | 廖吕燕 , 白丁平 , 秦韬 et al. 《中兽医学》实验课程教学改革探讨与实践 [J]. | 家畜生态学报 , 2021 , 42 (11) : 92-93,96 . |
| MLA | 廖吕燕 et al. "《中兽医学》实验课程教学改革探讨与实践" . | 家畜生态学报 42 . 11 (2021) : 92-93,96 . |
| APA | 廖吕燕 , 白丁平 , 秦韬 , 李健 . 《中兽医学》实验课程教学改革探讨与实践 . | 家畜生态学报 , 2021 , 42 (11) , 92-93,96 . |
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本发明提供的是一种鸡传染性法氏囊病病毒(IBDV)多抗原表位蛋白及其应用，所述蛋白其氨基酸序列如SEQ ID NO.1所示，本申请将鸡免疫球蛋白IgG重链部分恒定区(γCH)作为多表位融合蛋白的载体蛋白，将IBDV代表性毒株VP2和VP3上的B细胞表位和T细胞表位基因进行串联合成后与γCH编码基因相连接，构建重组质粒pET‑VP2+3‑γCH，并转化至大肠杆菌BL21中进行表达。重组多抗原表位融合蛋白pVP2+3‑γCH经过纯化和鉴定后通过SPF鸡进行的免疫攻毒保护试验，初步显示出了良好的免疫保护效果。本研究成功构建和表达重组多抗原表位蛋白pVP2+3‑γCH，为IBDV多表位疫苗的研发奠定基础。
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| GB/T 7714 | 吴异健 , 陈强 , 刘珍妮 et al. 一种鸡传染性法氏囊病病毒多抗原表位蛋白及其应用 : CN202011099954.4[P]. | 2020-10-15 . |
| MLA | 吴异健 et al. "一种鸡传染性法氏囊病病毒多抗原表位蛋白及其应用" : CN202011099954.4. | 2020-10-15 . |
| APA | 吴异健 , 陈强 , 刘珍妮 , 李明慧 , 廖吕燕 , 李娜 . 一种鸡传染性法氏囊病病毒多抗原表位蛋白及其应用 : CN202011099954.4. | 2020-10-15 . |
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本发明属于生物技术领域，具体涉及一种鸡毒支原体多抗原表位融合蛋白pMG‑mEA及其应用，所述pMG‑mEA其氨基酸序列如SEQ ID NO.1所示。pMG‑mEA融合蛋白包含鸡毒支原体CrmA、Gapa、Mgc2、PvpA等多个粘附素B细胞和T细胞抗原表位，能诱导体液免疫和细胞免疫，同时融合有鸡三肽囊素，具有免疫增强作用；此外原核表达pMG‑mEA融合蛋白的制备成本低，构建好表达质粒后可以大批量工业化表达生产，易于纯化。该鸡毒支原体多抗原表位融合蛋白pMG‑mEA的构建表达为临床预防鸡慢性呼吸道疾病(CRD)的发生奠定基础。
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| GB/T 7714 | 吴异健 , 刘秉珲 , 吴春琳 et al. 一种鸡毒支原体多抗原表位融合蛋白pMG-mEA及其应用 : CN202011099953.X[P]. | 2020-10-15 . |
| MLA | 吴异健 et al. "一种鸡毒支原体多抗原表位融合蛋白pMG-mEA及其应用" : CN202011099953.X. | 2020-10-15 . |
| APA | 吴异健 , 刘秉珲 , 吴春琳 , 严萍 , 廖吕燕 , 张立根 . 一种鸡毒支原体多抗原表位融合蛋白pMG-mEA及其应用 : CN202011099953.X. | 2020-10-15 . |
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