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学者姓名：王磊
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Abstract ：
Coccidiosis is a major parasitic disease that suppresses poultry productivity and causes significant global economic losses. Currently, controlling Eimeria parasites relies primarily on the use of anticoccidial drugs or live vaccines. However, these conventional control strategies face the dual constraints of escalating drug resistance and unsustainable economic expenditures. In this study, the efficacy of a chimeric subunit vaccine comprising Eimeria maxima Elongation Factor-1 alpha (EmEF1 alpha) and chicken chemokine Ligand-1 (ChXCL1) was assessed for protection against experimental Eimeria maxima infection. The synthetic gene fragment ChXCL1-EmEF1 alpha was ligated to the pET28a vector and expressed in vitro. Western blot analysis confirmed the successful expression of the recombinant ChXCL1-EmEF1 alpha protein. Chickens immunized with the ChXCL1-EmEF1 alpha exhibited a significantly stronger IgY response and higher secretion of IL-2 and IL-17 compared to those vaccinated with recombinant ChXCL1 alone or challenged solely with E. maxima. Furthermore, the ChXCL1-EmEF1 alpha group demonstrated enhanced anticoccidial effects, including reduced intestinal lesions, higher body weight gain, and lower oocyst shedding compared to control groups. Following E. maxima challenge, the EmEF1 alpha and ChXCL1-EmEF1 alpha groups demonstrated robust protective efficacy, achieving high ACI values of 182 and 178, respectively. In contrast, the ChXCL1 and UC groups exhibited significantly lower ACI values (150 and 149, respectively), indicating minimal protection. This improvement was also reflected in the immune response, with significantly elevated levels of CD4+ and CD8+ T cells in the ChXCL1-EmEF1 alpha-treated chickens. Moreover, ChXCL1 acts as an effective adjuvant when fused with EmEF1 alpha, enhancing the vaccine's anticoccidial efficacy. These results suggest that the ChXCL1-EmEF1 alpha chimeric immunogen is a promising candidate for developing subunit vaccines against E. maxima infections.
Keyword ：
adjuvant adjuvant chicken chicken Eimeria Eimeria immune response immune response subunit vaccine subunit vaccine
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| GB/T 7714 | Chen, Rong , Lin, Xiao-Feng , Wu, Hong-Yan et al. Synergistic Enhancement of Eimeria maxima Vaccine Efficacy Through EF-1α Antigen and Chicken XCL1 Chemokine Adjuvant Combination [J]. | ANIMALS , 2025 , 15 (16) . |
| MLA | Chen, Rong et al. "Synergistic Enhancement of Eimeria maxima Vaccine Efficacy Through EF-1α Antigen and Chicken XCL1 Chemokine Adjuvant Combination" . | ANIMALS 15 . 16 (2025) . |
| APA | Chen, Rong , Lin, Xiao-Feng , Wu, Hong-Yan , Li, Li-Na , Wang, Lei , Wang, Deng-Feng et al. Synergistic Enhancement of Eimeria maxima Vaccine Efficacy Through EF-1α Antigen and Chicken XCL1 Chemokine Adjuvant Combination . | ANIMALS , 2025 , 15 (16) . |
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Cinnamon (Cinnamomum cassia (L.) D. Don) is a commonly used drug in traditional Chinese medicine; it is especially useful for improving the reproductive performance in males and females. Cinnamaldehyde is the main component of cinnamon, which has bactericidal and disinfectant properties, promotes gastrointestinal motility and regulates testosterone secretion in the male. The present study aimed to investigate the mechanisms by which cinnamaldehyde regulates testosterone secretion in male animals. For this purpose, four-month-old male mice (n=25) were randomly divided into five groups (n=5 each) and treated with PBS, DMSO and three doses of cinnamaldehyde (25, 50 and 75mg/kg) by gavage for 2 weeks. Histological (IHC), ELISA and molecular analyses (WB, RTqPCR) were used to assess effects of cinnamaldehyde on murine liver, serum testosterone levels, and testicular enzymes associated with testosterone production. The mechanisms by which cinnamaldehyde affects testosterone secretion in testis Leydig cells were explored using ELISA, flow cytometry, WB, fluorescent quantitative PCR, and dual luciferase reporter systems. The results showed that cinnamaldehyde elevated murine body weight, serum testosterone, testicular interstitial cell density, and steroidogenic enzyme expression (StAR, CYP11A1, 3 beta- HSD) without altering hepatic tissue and testicular indices. Cinnamaldehyde treatment at 5x10-5M increased the expression of steroid hormone synthase in Leydig cells, increased the proportion of cells in the S phase, significantly increased the promoter activity of the steroid hormone synthesis nuclear receptor steroidogenic factor 1 (SF-1), and up-regulated the expression of the nuclear receptor SF-1. The present study showed that cinnamaldehyde can upregulate the expression of steroid hormone synthase by increasing the activity of the steroid hormone synthesis nuclear receptor promoter, which in turn increases the synthesis and secretion of testosterone from testicular interstitial cells.
Keyword ：
Cinnamaldehyde Cinnamaldehyde Leydig cells Leydig cells Nuclear receptors Nuclear receptors Steroidogenic-related genes Steroidogenic-related genes Testosterone Testosterone
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| GB/T 7714 | Meng, Qingrui , Huang, Xiaoyu , Cai, Congguang et al. The Effect and Mechanism of Cinnamaldehyde on Testosterone Secretion in Murine Model [J]. | PAKISTAN VETERINARY JOURNAL , 2025 , 45 (2) : 683-692 . |
| MLA | Meng, Qingrui et al. "The Effect and Mechanism of Cinnamaldehyde on Testosterone Secretion in Murine Model" . | PAKISTAN VETERINARY JOURNAL 45 . 2 (2025) : 683-692 . |
| APA | Meng, Qingrui , Huang, Xiaoyu , Cai, Congguang , Guo, Yuxin , Yang, Yang , Huang, Zhijian et al. The Effect and Mechanism of Cinnamaldehyde on Testosterone Secretion in Murine Model . | PAKISTAN VETERINARY JOURNAL , 2025 , 45 (2) , 683-692 . |
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| GB/T 7714 | Yang, Yang , Deng, Xin , Chen, Zhanghang et al. Dissolving cinnamaldehyde HA/PVA microneedles for the treatment of skin diseases caused by Microsporum canis (107, 106841, 2025) [J]. | JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY , 2025 , 113 . |
| MLA | Yang, Yang et al. "Dissolving cinnamaldehyde HA/PVA microneedles for the treatment of skin diseases caused by Microsporum canis (107, 106841, 2025)" . | JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY 113 (2025) . |
| APA | Yang, Yang , Deng, Xin , Chen, Zhanghang , Lin, Xiaofeng , Hu, Yuhao , Zeng, Qihui et al. Dissolving cinnamaldehyde HA/PVA microneedles for the treatment of skin diseases caused by Microsporum canis (107, 106841, 2025) . | JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY , 2025 , 113 . |
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[目的]旨在利用菌丝生长抑制试验、网络药理学和分子对接，分析丁香治疗真菌性皮肤病的潜能。[方法]通过菌丝生长抑制试验分析丁香对犬小孢子菌的体外抑菌活性。通过TCMSP数据库获取丁香的活性成分和相关作用靶点。利用GeneCards和OMIM数据库获取真菌性皮肤病的相关靶点。利用String数据库、Cytoscape3.7.2软件、AutodockTools软件、PyMOL软件构建植物提取物-活性成分-共有靶点-疾病网络关系图、蛋白相互作用（PPI）网络模型、GO功能、KEGG通路富集分析和分子对接。[结果]菌丝生长抑制试验结果表明，丁香提取物各浓度梯度处理组均对犬小孢子菌的生长具有显著抑制效果，呈浓度依赖性。网络药理学结果显示，丁香具有16种有效成分，有效成分对应靶点185个，有效成分靶点与疾病靶点取交集得到关键靶点123个；GO功能富集分析结果显示，RNA聚合酶II正向调控转录、细胞核、蛋白结合显著性最大；KEGG通路富集分析结果显示，丁香治疗真菌性皮肤病主要作用于癌症、TNF和IL-17信号通路。分子对接结果显示，各有效成分与关键靶点连接良好，其中AKT1和IL-6与有效成分连接亲和力最强。[结论]通过菌丝生长抑制试验和网络药理学分析，发现丁香不仅具备体外抗犬小孢子菌活性，而且可能会通过多靶点通路从抗炎途径发挥治疗真菌性皮肤病的作用，可为进一步研究丁香治疗真菌性皮肤病提供理论基础。
Keyword ：
丁香 丁香 分子对接 分子对接 犬小孢子菌 犬小孢子菌 真菌性皮肤病 真菌性皮肤病 网络药理学 网络药理学
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| GB/T 7714 | 谢珲洪 , 郭雨欣 , 张裕枨 et al. 基于网络药理学和分子对接技术探究丁香治疗真菌性皮肤病的作用机制 [J]. | 福建畜牧兽医 , 2025 , 47 (02) : 41-48,51 . |
| MLA | 谢珲洪 et al. "基于网络药理学和分子对接技术探究丁香治疗真菌性皮肤病的作用机制" . | 福建畜牧兽医 47 . 02 (2025) : 41-48,51 . |
| APA | 谢珲洪 , 郭雨欣 , 张裕枨 , 胡宇豪 , 陈韩钰 , 罗奕 et al. 基于网络药理学和分子对接技术探究丁香治疗真菌性皮肤病的作用机制 . | 福建畜牧兽医 , 2025 , 47 (02) , 41-48,51 . |
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Microsporum canis (M. cains) skin diseases are zoonotic, which cause serious diseases. Therefore, it is crucial to prevent and treat M. cain's skin diseases in companion animals for public health security and animal welfare. This study analyzed the potential anti-inflammatory mechanisms of Cinnamaldehyde (CA) in treating M. cains skin diseases through network pharmacology and molecular docking. CA was formulated into a dissolving microneedle for application. The findings suggest that CA may interact with Interleukin-1(3 (IL-1(3), Cellular tumor antigen p53 (P53), Microtubule-Associated Protein Kinase 14 (P38 alpha), and Tumor necrosis factor (TNF) to target pathways such as TNF signaling, Toll-like receptor signaling, and IL-17 signaling, ultimately reducing skin inflammation associated with M. cains skin diseases. Microneedles administration significantly enhances efficacy compared to traditional delivery methods. In vivo studies demonstrated that the efficacy of CA-HA/PVA DMNs was comparable to that of TER-HA/PVA DMNs. Compared to the use of DMNs alone, the symptom score decline index (SSDI) in the TER-HA/PVA DMNs group (TM group) and CA-HA/PVA DMNs group (CM group) increased by 25.01 % and 21.1 %, respectively, and both were superior to TER treatment alone. CA-HA/PVA DMNs and TER-HA/PVA DMNs effectively alleviate skin erythema, reduce dander production, and decrease skin keratinization at the superficial level. Moreover, at the histopathological level, they are successful in preventing fungal invasion of hair follicles. In summary, this study introduces a novel approach for treating M. cains skin diseases in companion animals.
Keyword ：
Cinnamaldehyde Cinnamaldehyde Fungal skin diseases Fungal skin diseases Microneedles Microneedles Microsporum canis Microsporum canis Network pharmacology Network pharmacology Treatment Treatment
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| GB/T 7714 | Yang, Yang , Deng, Xin , Chen, Zhanghang et al. Dissolving cinnamaldehyde HA/PVA microneedles for the treatment of skin diseases caused by Microsporum canis [J]. | JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY , 2025 , 107 . |
| MLA | Yang, Yang et al. "Dissolving cinnamaldehyde HA/PVA microneedles for the treatment of skin diseases caused by Microsporum canis" . | JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY 107 (2025) . |
| APA | Yang, Yang , Deng, Xin , Chen, Zhanghang , Lin, Xiaofeng , Hu, Yuhao , Zeng, Qihui et al. Dissolving cinnamaldehyde HA/PVA microneedles for the treatment of skin diseases caused by Microsporum canis . | JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY , 2025 , 107 . |
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This experiment investigated the effects of seaweed polysaccharide (SP) and seaweed enzymatic hydrolysate (SEH) on the growth performance, serum biochemical indices, antioxidant capacity, and intestinal function of Muscovy ducks. A total of 240 healthy 1 day female Muscovy ducks (48.85 +/- 0.45 g) were randomly divided into 3 treatment groups, with 4 replicates per group and 20 ducks per replicate. The control (CON) group received a basic diet supplemented with 20 mL/kg of water, the SP group received a basic diet supplemented with 20 mL/kg of SP, and the SEH group received a basic diet supplemented with 20 mL/kg of SEH. The experimental period lasted for 28 d. The results indicate that, compared to the CON group, the average daily feed intake (ADFI) and feed to gain (F/G) of the SP and SEH groups of ducks significantly decreased at 28 d (p < 0.05). In the SP group, serum levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST), as well as the concentrations of glucose (GLU), triglycerides (TG), total cholesterol (TCHO), low-density lipoprotein cholesterol (LDL-C), and high-density lipoprotein cholesterol (HDL-C), were significantly reduced (p < 0.05). In the SEH group, the activities of ALT and AST were also significantly lower (p < 0.05). Additionally, serum total antioxidant capacity (T-AOC) levels and superoxide dismutase (SOD) activity in the SEH group were significantly higher than those in the CON group (p < 0.05), while the malondialdehyde (MDA) content was significantly reduced (p < 0.05). Compared to the CON group, serum levels of immunoglobulin A (IgA), immunoglobulin G (IgG), interleukin-4 (IL-4), and interleukin-10 (IL-10) in the SP group were significantly increased (p < 0.05), whereas the levels of tumor necrosis factor-alpha (TNF-alpha), interleukin-1 beta (IL-1 beta), and interleukin-6 (IL-6) were significantly decreased (p < 0.05). In the SP and SEH groups, the villus height (VH) and the villus height to crypt depth (V/C) of the Muscovy ducks significantly increased (p < 0.05), while the crypt depth (CD) significantly decreased (p < 0.05). A significant increase in the abundance of Barnesiella was observed in the SP and SEH groups (p < 0.05), whereas the abundances of UCG-005 and Romboutsia significantly decreased (p < 0.05). LEfSe analysis indicated that g__Bacillus and g__Veillonella were significantly abundant in the SP group (p < 0.05), while g__Coriobacteriaceae_UCG_002 was significantly abundant in the SEH group (p < 0.05). In summary, the addition of SP and SEH to the feed can promote the healthy growth of ducks by improving intestinal morphology, regulating the structure of intestinal microbiota, enhancing antioxidant capacity and immune function, and optimizing metabolic indicators. This occurs while reducing feed intake and feed-to-weight ratio, and there is a certain specificity in their mechanisms of action.
Keyword ：
antioxidant capacity antioxidant capacity feed additives feed additives growth performance growth performance gut microbiome gut microbiome Muscovy ducks Muscovy ducks seaweed enzymatic hydrolysate (SEH) seaweed enzymatic hydrolysate (SEH) seaweed polysaccharide (SP) seaweed polysaccharide (SP)
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| GB/T 7714 | Wu, Hong-Yan , Lin, Xiao-Feng , Fu, Chang-Sheng et al. Effects of Seaweed Polysaccharide (SP) and Seaweed Enzymatic Hydrolysate (SEH) on Growth Performance, Antioxidant Capacity, Immune Function, and Gut Microbiota in Muscovy Ducks [J]. | ANIMALS , 2025 , 15 (20) . |
| MLA | Wu, Hong-Yan et al. "Effects of Seaweed Polysaccharide (SP) and Seaweed Enzymatic Hydrolysate (SEH) on Growth Performance, Antioxidant Capacity, Immune Function, and Gut Microbiota in Muscovy Ducks" . | ANIMALS 15 . 20 (2025) . |
| APA | Wu, Hong-Yan , Lin, Xiao-Feng , Fu, Chang-Sheng , Yang, Yang , Wang, Lei , Wu, Hai-Yan et al. Effects of Seaweed Polysaccharide (SP) and Seaweed Enzymatic Hydrolysate (SEH) on Growth Performance, Antioxidant Capacity, Immune Function, and Gut Microbiota in Muscovy Ducks . | ANIMALS , 2025 , 15 (20) . |
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为明确海藻酶解物饲喂大黄鱼的应用效果及对肠道菌群的影响，2022年11—12月在福建宁德海域使用添加0.5%复合海藻酶解物和0.5%海带酶解物的自配饲料饲养网箱养殖的大黄鱼8周，测定大黄鱼的存活率和平均增重，并采用NovaSeq PE250方案对肠道内容物进行16S rDNA扩增子测序，分析肠道菌群组成和特定菌群的相对丰度。结果显示：0.5%的复合海藻酶解物或0.5%的海带酶解物均可显著提高大黄鱼的存活率和平均增重（P<0.05）；肠道菌群分析显示0.5%复合海藻酶解物组大黄鱼肠道中扩增子序列变体（ASVs）达1 353种，以厚壁菌门（Firmicutes）、变形菌门（Proteobacteria）、拟杆菌门（Bacteroidota）、梭杆菌门（Fusobacteriota）和放线菌门（Actinobacteriota）为主，而0.5%海带酶解物组大黄鱼肠道中ASVs仅242种，各样本中以厚壁菌门和脱硫杆菌门（Desulfobacterota）为主，变形菌门相对丰度较低，均与对照组（749个ASVs，各样本中菌门相对丰度无规律）有较大差异；基于KEGG的肠道菌群功能分析显示在淀粉和蔗糖代谢、谷胱甘肽代谢、磷酸转移酶系统、氰基氨基酸代谢、亨廷顿病和其他糖降解上3组存在显著差异（P<0.05）；进一步分析发现，0.5%复合海藻酶解物增加了大黄鱼肠道菌群α多样性并提高了拟杆菌属及丁酸合成相关细菌的相对丰度，而0.5%海带酶解物则降低了大黄鱼肠道菌群α多样性，但可抑制肠道中假单胞菌属细菌的生长，且体外培养试验证实褐藻寡糖可极显著抑制变形假单胞菌生长（P<0.01）。综上可知，本试验制备的2种海藻酶解物可调节大黄鱼形成不同的肠道菌群结构，并均可促进大黄鱼的健康和生长，该结果可为大黄鱼海藻类饲料添加剂的开发和通过调整大黄鱼肠道菌群改善大黄鱼健康和生长的饲料添加剂研发提供数据参考。
Keyword ：
健康 健康 大黄鱼 大黄鱼 海藻酶解物 海藻酶解物 生长 生长 肠道菌群 肠道菌群
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| GB/T 7714 | 张露 , 熊宇明 , 董黎黎 et al. 海藻酶解物对大黄鱼生长和肠道菌群结构的影响 [J]. | 动物营养学报 , 2024 , 36 (03) : 1819-1833 . |
| MLA | 张露 et al. "海藻酶解物对大黄鱼生长和肠道菌群结构的影响" . | 动物营养学报 36 . 03 (2024) : 1819-1833 . |
| APA | 张露 , 熊宇明 , 董黎黎 , 梁萍 , 秦志清 , 王磊 et al. 海藻酶解物对大黄鱼生长和肠道菌群结构的影响 . | 动物营养学报 , 2024 , 36 (03) , 1819-1833 . |
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Technology transfer is an important demand for the development of veterinary medicine, and interdisciplinarity is of great significance in promoting the development of veterinary medicine. In recent years, as the star in the field of material science, hydrogel preparations have been widely used in biomedical fields and made a series of progress. Notable examples include wound management, drug delivery, and tissue engineering. However, the application of hydrogels in veterinary medicine is relatively rare, and therefore, only a few related studies have been reported. This paper reviews the classification, preparation materials, and methods of hydrogels, providing an overview of the current research status in the veterinary field. It further analyzes the potential applications of hydrogels in this area. Firstly, hydrogels demonstrate superior moisturizing properties, adequate mechanical strength, and appropriate surface microstructure and biochemical characteristics compared to traditional animal wound dressings, promoting the adhesion, proliferation, and differentiation of epidermal cells. Secondly, in the field of drug delivery, based on its unique structure and function, hydrogels have promising applications in animals for transdermal, oral and rectal mucosal administration. Lastly, in the field of animal tissue engineering, hydrogels have become a focal point of research due to their controllable physical and chemical properties, as well as their highly biomimetic characteristics that resemble the extracellular matrix of natural tissues. This review aims to enhance veterinary scholars' fundamental understanding of the advantages and applications of hydrogels and to elucidate potential future applications, thereby inspiring new research directions for the use of hydrogels in veterinary medicine.
Keyword ：
Drug delivery Drug delivery Hydrogels Hydrogels Skin wound treatment Skin wound treatment Tissue engineering Tissue engineering Veterinary medicine Veterinary medicine
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| GB/T 7714 | Yang, Yang , Cai, Congguang , Guo, Yuxin et al. Applications and Perspectives of Hydrogels in Veterinary Medicine [J]. | PAKISTAN VETERINARY JOURNAL , 2024 , 44 (4) : 998-1005 . |
| MLA | Yang, Yang et al. "Applications and Perspectives of Hydrogels in Veterinary Medicine" . | PAKISTAN VETERINARY JOURNAL 44 . 4 (2024) : 998-1005 . |
| APA | Yang, Yang , Cai, Congguang , Guo, Yuxin , Mohsin, Muhammad , Yin, Guangwen , Wang, Lei . Applications and Perspectives of Hydrogels in Veterinary Medicine . | PAKISTAN VETERINARY JOURNAL , 2024 , 44 (4) , 998-1005 . |
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本发明属于饲料和中兽药领域，具体涉及一种药食同源组合物及其制备方法和应用。本发明提供一种药食同源组合物，由甘草、金荞麦、牛蒡子制成。本发明药食同源组合物中金荞麦、甘草和牛蒡子能够达到协同增效的作用，能够取得提高猪的生长性能、降低料肉比、改善猪应激性、改善猪肝脏性能、提高猪免疫力、改善猪肠道菌群等效果。
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| GB/T 7714 | 王登峰 , 曾金哲 , 徐骏 et al. 一种药食同源组合物及其制备方法和应用 : CN202410110046.2[P]. | 2024-01-26 . |
| MLA | 王登峰 et al. "一种药食同源组合物及其制备方法和应用" : CN202410110046.2. | 2024-01-26 . |
| APA | 王登峰 , 曾金哲 , 徐骏 , 黄志坚 , 邓莉萍 , 胡宇豪 et al. 一种药食同源组合物及其制备方法和应用 : CN202410110046.2. | 2024-01-26 . |
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我国教育部高度重视慕课建设，提出了课程建设的“双万计划”。慕课建设和应用是实现我国高等教育公平，提高教育质量的关键一招。笔者以福建省精品慕课“兽医产科学”为例，介绍兽医类慕课建设和应用，为高校教师进行慕课建设、开展混合式教学提供参考。
Keyword ：
兽医产科学 兽医产科学 慕课 慕课 课程应用 课程应用 课程建设 课程建设
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| GB/T 7714 | 王磊 , 殷光文 , 王登峰 et al. “兽医产科学”精品慕课的建设与应用 [J]. | 当代畜牧 , 2023 , 2 (09) : 64-65 . |
| MLA | 王磊 et al. "“兽医产科学”精品慕课的建设与应用" . | 当代畜牧 2 . 09 (2023) : 64-65 . |
| APA | 王磊 , 殷光文 , 王登峰 , 江和基 , 黄志坚 . “兽医产科学”精品慕课的建设与应用 . | 当代畜牧 , 2023 , 2 (09) , 64-65 . |
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