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学者姓名：曾显成
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Abstract ：
H3N3 avian influenza viruses (AIVs) are less prevalent in poultry than H3N8 viruses. However, although relatively rare, reassortant H3N3 viruses have been known to appear in both domestic poultry and wild birds. In this study, we isolated the H3N3 virus in chickens sourced from a live poultry market in China. A comprehensive genomic analysis revealed that the virus possessed a single basic amino acid in the cleavage site of the hemagglutinin (HA) gene. Phylogenetic analysis indicated that eight genes in the H3N3 virus belong to the Eurasian lineage. Specifically, the HA and NA genes were clustered with H3N2 and H11N3, respectively, while the internal genes were closely related to the H3N8 and H9N2 viruses. Furthermore, the H3N3 virus exhibited high and moderate stability in thermal and acidic conditions and efficient replication capabilities in mammalian cells. The H3N3 virus demonstrated that it could infect and replicate in the upper and lower respiratory tract of BALB/c mice without prior adaptation, triggering hemagglutination inhibition (HI) antibody titres ranging from 80 to 160; notably, the H3N3 virus replicated vigorously within the chicken respiratory and digestive tracts. The virus also transmitted efficiently and swiftly among chickens through direct contact, leading to higher levels of HI antibodies in both the inoculated and contact birds. These findings suggest that the H3N3 virus may be a novel reassortant originating from viruses circulating in domestic poultry, thus demonstrating an increased pathogenicity and transmissibility in chickens. Our study determines that H3N3 AIV potentially threatens the poultry industry and public health, highlighting the importance of active surveillance of AIVs.
Keyword ：
avian influenza virus avian influenza virus evolution evolution pathogenicity pathogenicity transmissibility transmissibility Zoonoses Zoonoses
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| GB/T 7714 | Zhang, Chunping , Zhao, Conghui , Huang, Jiacheng et al. Emergence of a novel reassortant H3N3 avian influenza virus with enhanced pathogenicity and transmissibility in chickens in China [J]. | VETERINARY RESEARCH , 2025 , 56 (1) . |
| MLA | Zhang, Chunping et al. "Emergence of a novel reassortant H3N3 avian influenza virus with enhanced pathogenicity and transmissibility in chickens in China" . | VETERINARY RESEARCH 56 . 1 (2025) . |
| APA | Zhang, Chunping , Zhao, Conghui , Huang, Jiacheng , Wang, Yang , Jiang, Bo , Zheng, Hangyu et al. Emergence of a novel reassortant H3N3 avian influenza virus with enhanced pathogenicity and transmissibility in chickens in China . | VETERINARY RESEARCH , 2025 , 56 (1) . |
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为了解H6N8亚型禽流感病毒（AIV）的分布特征，本研究统计分析了NCBI和GISAID数据库中400株H6N8亚型AIV的宿主、分离年份和地理分布特征，结果显示，H6N8亚型AIV在全球范围内持续流行，主要在野鸟和家禽中分离到，中国南方分布较多。为进一步探究福建省H6N8亚型AIV的遗传进化特征，本研究于2023年在活禽市场采集285份家禽泄殖腔拭子样品，经接种鸡胚，37℃培养48 h后分离到1株AIV，经RT-PCR和测序分析鉴定为H6N8亚型AIV，并对其进行了全基因组测序、遗传进化分析和关键位点氨基酸序列分析。序列分析结果显示，该病毒HA蛋白裂解位点氨基酸序列为PQIETR↓GLF，无连续碱基氨基酸，符合低致病性AIV（LPAIV）的分子特征。HA蛋白
Keyword ：
H6N8亚型AIV H6N8亚型AIV 三间分布 三间分布 关键氨基酸 关键氨基酸 禽流感病毒 禽流感病毒 遗传进化 遗传进化
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| GB/T 7714 | 黄佳承 , 彭燕妮 , 郑航煜 et al. 一株重组H6N8亚型禽流感病毒的遗传进化分析 [J]. | 中国预防兽医学报 , 2025 , 47 (05) : 443-451 . |
| MLA | 黄佳承 et al. "一株重组H6N8亚型禽流感病毒的遗传进化分析" . | 中国预防兽医学报 47 . 05 (2025) : 443-451 . |
| APA | 黄佳承 , 彭燕妮 , 郑航煜 , 庄明智 , 张春萍 , 王洋 et al. 一株重组H6N8亚型禽流感病毒的遗传进化分析 . | 中国预防兽医学报 , 2025 , 47 (05) , 443-451 . |
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The H12 subtypes of avian influenza viruses (AIVs) are globally prevalent in wild birds, occasionally spilling over into poultry. In this study, we isolated an H12N8 virus from ducks in a live poultry market. Full genomic analysis revealed that the virus bears a single basic amino acid in the cleavage site of the hemagglutinin gene. Phylogenetic analysis revealed that the eight gene segments of the H12N8 virus belong to the Eurasian lineage and the HA gene was clustered with wild bird-originated H12 viruses, with its NP gene showing the highest nucleotide similarity to 2013-like H7N9 viruses. The H12N8 virus replicated effectively in both mammalian and avian cells without prior adaptation. Moreover, the H12N8 virus could infect and replicate in the upper respiratory tract of BALB/c mice without prior adaptation. The H12N8 virus replicated and transmitted inefficiently in both ducks and chickens and hardly triggered high hemagglutination inhibition (HI) antibody titers in the inoculated and contact animals. These results suggest that the wild bird-origin H12N8 virus has reassorted with viruses circulating in domestic poultry, but it inefficiently replicates and transmits in avian hosts. Our findings demonstrate that H12N8 AIV has emerged in domestic poultry, emphasizing the importance of active surveillance of AIVs in both wild and domestic birds.
Keyword ：
avian influenza virus avian influenza virus evolution evolution pathogenicity pathogenicity transmissibility transmissibility
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| GB/T 7714 | Zhao, Conghui , Huang, Jiacheng , Zhang, Chunping et al. Characteristics of the First Domestic Duck-Origin H12N8 Avian Influenza Virus in China [J]. | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES , 2025 , 26 (6) . |
| MLA | Zhao, Conghui et al. "Characteristics of the First Domestic Duck-Origin H12N8 Avian Influenza Virus in China" . | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 26 . 6 (2025) . |
| APA | Zhao, Conghui , Huang, Jiacheng , Zhang, Chunping , Wang, Yang , Zhang, Xiaoxuan , Liu, Sha et al. Characteristics of the First Domestic Duck-Origin H12N8 Avian Influenza Virus in China . | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES , 2025 , 26 (6) . |
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为了解福建省犬细小病毒主要流行趋势及其分子进化特点，对2019-2022年间采集的福建省部分地区疑似感染犬细小病毒的犬粪便样品进行PCR检测，将阳性样品进行VP2全长基因组测序，并对其进行遗传进化分析。结果表明：收集的217份病料中犬细小病毒阳性样品有147份，感染率为67.74%。对其中41份阳性样品进行VP2全长基因组测序，有31株CPV-2c (占75.61%)、8株New CPV-2a (占19.51%)和2株New CPV-2b (占4.88%),说明2019-2022年间福建部分地区流行的犬细小病毒毒株亚型以CPV-2c为主。遗传进化分析表明，分离毒株有7个氨基酸位点发生突变；与我国2008-2022年间各亚型流行毒株属于同一个分支，各自有较高的亲缘关系，与美国、日本和意大利分离株亲缘关系较远，有明显的地域性。该研究丰富了犬细小病毒的基因组信息数据库，可为监测犬细小病毒的变异、地区流行趋势及疫苗研发提供一定参考。
Keyword ：
VP2基因 VP2基因 检测 检测 犬细小病毒 犬细小病毒 遗传进化 遗传进化
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| GB/T 7714 | 陆畅 , 王晓燕 , 林炫孜 et al. 福建部分地区犬细小病毒的检测与遗传进化分析 [J]. | 扬州大学学报(农业与生命科学版) , 2025 , 46 (02) : 16-24 . |
| MLA | 陆畅 et al. "福建部分地区犬细小病毒的检测与遗传进化分析" . | 扬州大学学报(农业与生命科学版) 46 . 02 (2025) : 16-24 . |
| APA | 陆畅 , 王晓燕 , 林炫孜 , 全锦洋 , 许宇擎 , 黄俏 et al. 福建部分地区犬细小病毒的检测与遗传进化分析 . | 扬州大学学报(农业与生命科学版) , 2025 , 46 (02) , 16-24 . |
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Introduction Classical Muscovy duck reovirus (C-MDRV) and goose-origin Muscovy duck reovirus (Go-MDRV) infections cause "Liver white-spots disease" in Muscovy duckling and gosling. It is difficult to differentiate the infections caused by C-MDRV and Go-MDRV using conventional serological methods.Methods Specific primers were designed and synthesized according to sigma NS and lambda A nucleotide sequences of C-MDRV and Go-MDRV, respectively. The PCR amplified products were cloned into the pMD-18-T vector. The recombinant plasmid DNA was used to establish an SYBR Green & Iukcy; based duplex real-time PCR assay for the simultaneous detection and differentiation of C-MDRV and Go-MDRV using high-resolution melting (HRM) analysis. The specificity, sensitivity, and repeatability of the methodology were examined based on the optimization of the reaction system and amplification conditions.Results C-MDRV and Go-MDRV were identified by their distinctive melting temperatures with 84.50 +/- 0.25 degrees C for C-MDRV and 87.50 +/- 0.20 degrees C for Go-MDRV, respectively. The amplifications were specific, and other non-targeted waterfowl viruses employed in this study did not show normalized melting peaks. The intra- and inter-assay coefficients of variations were between 0.05 and 1.83%, demonstrating good repeatability. The detection limits of this assay were 51.4 copiesmu l-1 for C-MDRV and 61.8 copiesmu l-1 for Go-MDRV, respectively. A total of 45 clinical samples were tested by RT-qPCR, with positive rates of 15.56% for C-MDRV and 22.22% for Go-MDRV, without co-infections.Discussion These results suggest that this duplex RT-qPCR method is highly sensitive, specific, and reproducible. The HRM assay established in this study provides a powerful tool for the differential detection and epidemiological investigation of C-MDRV and Go-MDRV.
Keyword ：
classical Muscovy duck reovirus classical Muscovy duck reovirus differential diagnosis differential diagnosis duplex real-time RT-PCR duplex real-time RT-PCR goose-origin Muscovy duck reovirus goose-origin Muscovy duck reovirus high-resolution melting high-resolution melting
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| GB/T 7714 | Xu, Zhuoran , Liu, Hongwei , Zheng, Xin et al. Simultaneous detection and differentiation of classical Muscovy duck reovirus and goose-origin Muscovy duck reovirus by RT-qPCR assay with high-resolution melting analysis [J]. | FRONTIERS IN VETERINARY SCIENCE , 2024 , 11 . |
| MLA | Xu, Zhuoran et al. "Simultaneous detection and differentiation of classical Muscovy duck reovirus and goose-origin Muscovy duck reovirus by RT-qPCR assay with high-resolution melting analysis" . | FRONTIERS IN VETERINARY SCIENCE 11 (2024) . |
| APA | Xu, Zhuoran , Liu, Hongwei , Zheng, Xin , Cheng, Xiaoxia , Wang, Shao , You, Guangju et al. Simultaneous detection and differentiation of classical Muscovy duck reovirus and goose-origin Muscovy duck reovirus by RT-qPCR assay with high-resolution melting analysis . | FRONTIERS IN VETERINARY SCIENCE , 2024 , 11 . |
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Duck spleen marble-like necrosis disease (DSMND) has been prevalent in Chinese duck farms since 2016. The etiological study was carried out in this study using etiological detection, pathogen isolation, whole genome sequencing, and artificial infection test. A highly pathogenic Riemerella anatipestifer (RA) strain was determined as the etiologic agent. Phylogenomic analysis showed that this RA strain was closely related to duck origin RA strain RCAD0421 and chicken origin RA strain S63. The clinical symptoms and pathological changes of artificial infection ducks were similar to those of clinical cases. This is the first identification of RA as the pathogen of DSMND, which provides a theoretical basis for this disease' s prevention and control.
Keyword ：
Duck spleen marble-like necrosis Duck spleen marble-like necrosis Phylogenetic analysis Phylogenetic analysis Riemerella anatipestifer Riemerella anatipestifer
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| GB/T 7714 | Chen, Shilong , Zeng, Xiancheng , Zheng, Xin et al. Identification of a highly pathogenic Riemerella anatipestifer strain causing duck spleen marble-like necrosis disease in China [J]. | MICROBIAL PATHOGENESIS , 2024 , 196 . |
| MLA | Chen, Shilong et al. "Identification of a highly pathogenic Riemerella anatipestifer strain causing duck spleen marble-like necrosis disease in China" . | MICROBIAL PATHOGENESIS 196 (2024) . |
| APA | Chen, Shilong , Zeng, Xiancheng , Zheng, Xin , Lin, Chang , Jiang, Bin , Zhu, Xiaoli et al. Identification of a highly pathogenic Riemerella anatipestifer strain causing duck spleen marble-like necrosis disease in China . | MICROBIAL PATHOGENESIS , 2024 , 196 . |
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【目的】通过对雏鹅以肝脾白色坏死点为特征的病料进行病原分离与鉴定，为该病的防控提供科学依据。【方法】利用PCR检测排查病原，挑选阳性病料接种番鸭胚和番鸭成纤维细胞（muscovy duck embryo fibroblast cells,MDEF）进行病毒分离，对分离毒再进行RT-PCR鉴定、病毒关键基因序列分析、动物回归试验验证。【结果】成功分离出一株鹅源番鸭呼肠孤病毒（goose-origin muscovy reovirus, Go-MDRV），命名为JS2022株。分离毒接种番鸭胚至第6代时，番鸭胚死亡时间稳定在3～5 d，胚体出血，肝脏有大小不一的出血点和坏死灶，发育受阻。将分离毒尿囊液接种MDEF，出现细胞圆缩、崩解等病变。将JS2022株σB和σC基因的核苷酸序列与鹅源番鸭呼肠孤病毒、番鸭呼肠弧病毒（muscovy duck reovirus, MDRV）、新型鸭呼肠孤病毒（novel duck reovirus, NDRV）和禽呼肠孤病毒（avian reovirus, ARV）的毒株序列进行同源性比对，结果显示JS2022株与鹅源番鸭呼肠孤病毒毒株的同源性最高，核苷酸同源性范围分别为99.1%～99.5%和99.3%～99.9%；动物回归试验成功复制出与临床发病鹅相同的病症。【结论】本试验成功从病死鹅的肝脏和脾脏中分离鉴定1株鹅源番鸭呼肠孤病毒JS2022株，为鹅源番鸭呼肠孤病毒病的防治提供参考。
Keyword ：
σB基因 σB基因 σC基因 σC基因 分离 分离 同源性比较 同源性比较 鉴定 鉴定 鹅源番鸭呼肠孤病毒 鹅源番鸭呼肠孤病毒
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| GB/T 7714 | 郑欣 , 胥焯然 , 宫晓 et al. 一株以肝脾白色坏死点为特征的鹅源番鸭呼肠孤病毒的分离鉴定 [J]. | 福建农业学报 , 2024 , 39 (10) : 1152-1161 . |
| MLA | 郑欣 et al. "一株以肝脾白色坏死点为特征的鹅源番鸭呼肠孤病毒的分离鉴定" . | 福建农业学报 39 . 10 (2024) : 1152-1161 . |
| APA | 郑欣 , 胥焯然 , 宫晓 , 江丹丹 , 程晓霞 , 朱小丽 et al. 一株以肝脾白色坏死点为特征的鹅源番鸭呼肠孤病毒的分离鉴定 . | 福建农业学报 , 2024 , 39 (10) , 1152-1161 . |
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【目的】研究新型鸭呼肠孤病毒（Novel duck reovirus,NDRV）弱毒S株C30在雏番鸭血液中的病毒血症，口咽、泄殖腔的排毒规律及靶器官的带毒时间，了解弱毒S株在雏番鸭体内的增殖规律和致弱机理。【方法】设计特异性引物，建立检测NDRV核酸的RT-qPCR方法；新型鸭呼肠孤病毒弱毒S株第30代（NDRV-S-C30）腿部肌肉注射2日龄雏番鸭，在免疫后1、2、3、4、6、8、10、12、14 d(Days post vaccination,dpv)采集其血液、肝脏、脾脏、泄殖腔分泌液和口咽液，用RT-qPCR方法检测NDRV在血液、口咽、泄殖腔及靶器官的增殖能力及带毒时间。【结果】建立了检测NDRV核酸的特异性RT-qPCR方法，使用该方法检测NDRV弱毒攻毒雏鸭的口咽和泄殖腔排毒时间分别为2～8 d和2～10 d，排毒高峰期为4～6 d；病毒血症时间为1～4 d，其中1～2 d为病毒血症高峰期；弱毒在肝脏的带毒时间为3～6 d，在脾脏的带毒时间为1～8 d。【结论】NDRV-S-C30弱毒株免疫雏番鸭后可通过口腔和泄殖腔向外界排毒，仅能引起较弱的病毒血症反应，且在肝脏中的增殖能力弱，丧失了对易感靶器官造成病理损伤的能力。明确了NDRV弱毒S株的排毒规律及病毒血症时间，为建立弱毒免疫效力评价方法奠定了基础。
Keyword ：
RT-qPCR RT-qPCR 弱毒株 弱毒株 排毒规律 排毒规律 新型鸭呼肠孤病毒 新型鸭呼肠孤病毒 病毒血症 病毒血症
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| GB/T 7714 | 胥焯然 , 程晓霞 , 郑敏 et al. 新型鸭呼肠孤病毒弱毒S株在雏番鸭体内的增殖规律 [J]. | 福建农业学报 , 2023 , 38 (09) : 1011-1016 . |
| MLA | 胥焯然 et al. "新型鸭呼肠孤病毒弱毒S株在雏番鸭体内的增殖规律" . | 福建农业学报 38 . 09 (2023) : 1011-1016 . |
| APA | 胥焯然 , 程晓霞 , 郑敏 , 朱小丽 , 董慧 , 肖世峰 et al. 新型鸭呼肠孤病毒弱毒S株在雏番鸭体内的增殖规律 . | 福建农业学报 , 2023 , 38 (09) , 1011-1016 . |
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细菌病综合实习是提升动物医学专业预防兽医学实践教学质量的重要环节。针对传统预防兽医学中细菌学实验内容零碎、不系统的现状，本文提出融合细菌学相关实验技术，开展连续性好、综合性强的细菌病综合实习。该实习从混合细菌感染动物人工造病开始，通过一系列实验技术手段分离、纯化和鉴定细菌，进而确诊病因，实施后显著提升了学生的综合实践能力和科研创新水平，有利于新时代高素质动物医学专业人才的培养。
Keyword ：
动物医学 动物医学 效果分析 效果分析 细菌病 细菌病 综合实习 综合实习 预防兽医学 预防兽医学
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| GB/T 7714 | 曾显成 , 池雪林 , 王松 et al. 预防兽医学细菌病综合实习的设计与实践 [J]. | 中国兽医杂志 , 2023 , 59 (03) : 154-157 . |
| MLA | 曾显成 et al. "预防兽医学细菌病综合实习的设计与实践" . | 中国兽医杂志 59 . 03 (2023) : 154-157 . |
| APA | 曾显成 , 池雪林 , 王松 , 陈叶 , 黄志坚 . 预防兽医学细菌病综合实习的设计与实践 . | 中国兽医杂志 , 2023 , 59 (03) , 154-157 . |
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Cyclic GMP-AMP Synthase (cGAS) is a pivotal adaptor of the signaling pathways involving the pattern recognition receptors and plays an important role in apoptosis and immune regulation. The cGAS function in mammals has been investigated extensively; however, the function of duck cGAS (du-cGAS) in response to viral infections is still unclear. This study aimed to clone the mallard (Anas platyrhynchos) cGAS homolog to investigate the function of duck cGAS (du-cGAS) in host antiviral innate immunity. The results showed that the open reading frame (ORF) region of the du-cGAS gene was 1296 bp, encoding 432 amino acids (aa) and exhibiting similar functional domains with its chicken counterpart. Knockdown of the endogenous du-cGAS by specific sgRNA strongly increased the replication of DNA viruses, including duck adenovirus B2 (DAdV B2) and duck short beak and dwarfism syndrome virus (SBDSV). However, the knockout did not impair the replication of novel duck reovirus (NDRV), an RNA virus. Furthermore, the mRNA expressions of type I interferon (IFNs) and vital interferon-stimulated genes (ISGs) were remarkably reduced in the du-cGAS knockout DEF cell line. Inversely, du-cGAS overexpression greatly activated the transcription of IFN-alpha, IFN-beta, and vital ISGs, and impaired the replication of DAdV B2, SBDSV, and NDRV in the DEF cell line. Importantly, we found that a deletion of 68 aa in the N terminus didn't impair the antiviral function of du-cGAS. Overexpressing NTase Core, C-Domain (Mab21), or Zinc-Ribbon domain independently had no antiviral effects. Generally, these results reveal that du-cGAS is a vital component of the innate immune system of ducks, with a universal antiviral activity, and provides a useful strategy for the control of waterfowl viral diseases.
Keyword ：
antiviral function antiviral function cyclic GMP-AMP synthase cyclic GMP-AMP synthase duck duck innate immunity innate immunity interferon-stimulated genes interferon-stimulated genes sgRNA sgRNA
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| GB/T 7714 | Lin, Chang , Zheng, Min , Xiao, Shifeng et al. Duck cGAS inhibits DNA and RNA virus replication by activating IFNs and antiviral ISGs [J]. | FRONTIERS IN IMMUNOLOGY , 2023 , 14 . |
| MLA | Lin, Chang et al. "Duck cGAS inhibits DNA and RNA virus replication by activating IFNs and antiviral ISGs" . | FRONTIERS IN IMMUNOLOGY 14 (2023) . |
| APA | Lin, Chang , Zheng, Min , Xiao, Shifeng , Wang, Shao , Zhu, Xiaoli , Chen, Xiuqin et al. Duck cGAS inhibits DNA and RNA virus replication by activating IFNs and antiviral ISGs . | FRONTIERS IN IMMUNOLOGY , 2023 , 14 . |
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