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学者姓名：俞道进
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Abstract ：
【目的】了解猪源性肠球菌的耐药性及肠球菌对重金属锌抗性的现状，探究耐药基因和锌抗性基因的流行特征及其之间的相关性，为后期养殖临床上抗菌药物和ZnO的使用提供参考。【方法】采集了395份猪粪便样品，使用特异性选择培养基分离肠球菌，采用微量肉汤稀释法测定肠球菌分离株对9种抗菌药物和ZnSO
Keyword ：
抗锌性 抗锌性 氧化锌 氧化锌 猪源肠球菌 猪源肠球菌 耐药基因 耐药基因 耐药性 耐药性
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| GB/T 7714 | 宋辉辉 , 孙志华 , 洪娟 et al. 猪源性肠球菌耐药性与抗锌性的相关性 [J]. | 福建农林大学学报(自然科学版) , 2025 , 54 (01) : 100-109 . |
| MLA | 宋辉辉 et al. "猪源性肠球菌耐药性与抗锌性的相关性" . | 福建农林大学学报(自然科学版) 54 . 01 (2025) : 100-109 . |
| APA | 宋辉辉 , 孙志华 , 洪娟 , 赖华敏 , 任子宁 , 易灵娴 et al. 猪源性肠球菌耐药性与抗锌性的相关性 . | 福建农林大学学报(自然科学版) , 2025 , 54 (01) , 100-109 . |
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【目的】建立一种猪A群轮状病毒（porcine rotavirus group A, PoRV A）快速检测方法，用于PoRV检测和流行病学调查。【方法】参考GenBank中猪A群轮状病毒（PoRVA）VP6基因序列（登录号MT025937.1、OP978242.1、PP566178.1）设计特异性引物和探针，优化反应体系中引物和探针的浓度，建立Taq Man RT-qPCR检测方法，并通过特异性、灵敏性和重复性的结果以及临床应用对该方法进行评价。【结果】该方法可特异性扩增PoRV核酸，最低检出限度为27.0 copies·μL
Keyword ：
Taq Man探针 Taq Man探针 猪A群轮状病毒 猪A群轮状病毒 荧光定量RT-PCR 荧光定量RT-PCR
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| GB/T 7714 | 林青 , 康龙滨 , 吴瑞森 et al. 猪A群轮状病毒Taq Man荧光定量RT-PCR检测方法的建立与运用 [J]. | 福建农业学报 , 2025 , 40 (04) : 370-376 . |
| MLA | 林青 et al. "猪A群轮状病毒Taq Man荧光定量RT-PCR检测方法的建立与运用" . | 福建农业学报 40 . 04 (2025) : 370-376 . |
| APA | 林青 , 康龙滨 , 吴瑞森 , 赵文娟 , 陈秋勇 , 王隆柏 et al. 猪A群轮状病毒Taq Man荧光定量RT-PCR检测方法的建立与运用 . | 福建农业学报 , 2025 , 40 (04) , 370-376 . |
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The study aimed to provide a possible strategy to evaluate the detoxifying efficacy of mycotoxin adsorbents in vivo by analyzing deoxynivalenol (DON) concentration in feces. Fifteen pigs were randomly assigned to five groups (groups A-E, 3 replicates/group). The pigs in each group were fed twice a day for 10 d with 500 g of designed diets (group A, commercial feedstuffs; group B, DON-contaminated (mildewed) feedstuffs; groups C, D, E, mildewed feedstuffs containing 0.2% adsorbent 1, 2, and 3, respectively). For each pig, 2-g fecal samples were collected pre-feeding and analyzed by ultra-performance liquid chromatography. Nondetectable or low concentrations of DON (<1.38 mu g/g) were found in fecal samples from groups A and B. High concentrations of DON (>20 mu g/g) were detected in six out of twenty fecal batches from pigs in group C. Moderate concentrations of DON (5.54-6.50 mu g/g) were detected in one out of twenty fecal batches from pigs in group D and two out of twenty in group E. Based on the predefined evaluation criteria, higher DON concentration and frequency in feces indicate better adsorbent efficacy. Notably, Absorbent 1 demonstrated a more pronounced detoxification efficacy in vivo compared to the other two absorbents.
Keyword ：
deoxynivalenol deoxynivalenol fecal sample fecal sample in vivo evaluation of detoxification efficacy of mycotoxin adsorbent in vivo evaluation of detoxification efficacy of mycotoxin adsorbent ultra-performance liquid chromatography ultra-performance liquid chromatography
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| GB/T 7714 | Yang, Bo , Deng, Hui , Jia, Yiwei et al. Validation of a Methodology for the Quantification of DON in Feces and Feedstuffs by UPLC as Possible Strategy to Evaluate the Detoxifying Efficacy of a Mycotoxin Adsorbent In Vivo [J]. | TOXINS , 2025 , 17 (7) . |
| MLA | Yang, Bo et al. "Validation of a Methodology for the Quantification of DON in Feces and Feedstuffs by UPLC as Possible Strategy to Evaluate the Detoxifying Efficacy of a Mycotoxin Adsorbent In Vivo" . | TOXINS 17 . 7 (2025) . |
| APA | Yang, Bo , Deng, Hui , Jia, Yiwei , Li, Dong , Chen, Rudeng , Chen, Ruiqing et al. Validation of a Methodology for the Quantification of DON in Feces and Feedstuffs by UPLC as Possible Strategy to Evaluate the Detoxifying Efficacy of a Mycotoxin Adsorbent In Vivo . | TOXINS , 2025 , 17 (7) . |
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Matrine (MT) is a potential resistance reversal agent. However, little is known about its pharmacokinetics (PK) in pigs. This study aimed to investigate the PK of MT in pigs after gavage administration alone and in combination with amoxicillin (AMO). Twenty-four pigs were randomly assigned to three groups: A (MT, 50 mg/kg), B (AMO, 50 mg/kg), and C (MT + AMO, 50 mg/kg each). Blood samples were collected at predetermined time points post-administration and analyzed using liquid chromatography-tandem mass spectrometry. PK parameters were calculated using a one-compartment model. The results showed that MT was absorbed and eliminated rapidly in pigs. The maximum concentration (Cmax), time to maximum concentration (Tmax), area under the curve from 0 to 36 h (AUC0-36 h), apparent clearance (Cl/F), elimination rate constant (ke), and absorption rate constant (ka) for group A were 1345.55 +/- 302.94 mu g/L, 2.03 +/- 0.14 h, 3979.10 +/- 1260.85 hmu g/L, 13.72 +/- 4.30 L/h/kg, 1.07 +/- 0.20 h-1, and 0.46 +/- 0.09 h-1, respectively, versus 2071.70 +/- 715.49 mu g/L, 1.27 +/- 0.36 h, 9113.80 +/- 3152.85 hmu g/L, 6.17 +/- 2.48 L/h/kg, 2.08 +/- 0.55 h-1, and 0.44 +/- 0.24 h-1 for group C. AMO significantly altered the PK profiles of MT.
Keyword ：
amoxicillin amoxicillin drug-drug interaction drug-drug interaction matrine matrine pharmacokinetics pharmacokinetics pig pig
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| GB/T 7714 | Li, Ruonan , Zhou, Danna , Hu, Huiyu et al. Pharmacokinetics of Matrine in Pigs After Gavage Administration of Matrine Alone and in Combination with Amoxicillin [J]. | ANIMALS , 2025 , 15 (17) . |
| MLA | Li, Ruonan et al. "Pharmacokinetics of Matrine in Pigs After Gavage Administration of Matrine Alone and in Combination with Amoxicillin" . | ANIMALS 15 . 17 (2025) . |
| APA | Li, Ruonan , Zhou, Danna , Hu, Huiyu , Wang, Fuhao , Lv, Xiaoling , Sun, Lei et al. Pharmacokinetics of Matrine in Pigs After Gavage Administration of Matrine Alone and in Combination with Amoxicillin . | ANIMALS , 2025 , 15 (17) . |
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BackgroundHeteroresistance represents a significant pathway through which sensitive bacteria evolve into resistant strains, posing challenges for current clinical laboratory detection methods.ObjectivesThis study aimed to investigate the differences in resistance among K. pneumoniae isolates from various sources, assess the prevalence of chloramphenicol heteroresistance (CHR), and explore the potential causes and key genes associated with CHR.MethodsK. pneumoniae was isolated from 801 samples obtained from various sources, and its susceptibility to antibacterial agents was assessed. The modified Kirby-Bauer disk diffusion method, population analysis profiling (PAP), and bactericidal curve assays were employed to identify heteroresistant bacteria. Additionally, the growth curve and stability of CHR strains were measured. To analyze the factors influencing the formation of CHR, we detected the resistance genes cmlA, cat1, and floR across 17 resistant subpopulations, along with virulence genes such as fimH, wabG, kfu, uge, and aerobactin.ResultsAmong the 198 K. pneumoniae tested, resistance rates to nitrofurantoin, tetracycline, and chloramphenicol were found to be 73.74%, 57.58%, and 51.01%, respectively. The prevalence of CHR was determined to be 8.59% (17 out of 198), which significantly diminished the in vitro bactericidal efficacy of chloramphenicol. Notably, 76.47% (13/17) of the isolates harbored the cat1 and/or floR genes, while the prevalence of the virulence genes wabG, fimH, uge, and kfu was 100%, 100%, 76.47%, and 47.06%, respectively.ConclusionThe floR and/or cat1 genes are pivotal in the mechanism underlying heteroresistance to chloramphenicol, and the presence of virulence genes could further contribute to the development of CHR.
Keyword ：
Chloramphenicol Chloramphenicol Heteroresistance Heteroresistance Klebsiella pneumoniae Klebsiella pneumoniae Resistance Resistance Subpopulations Subpopulations
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| GB/T 7714 | Kuang, Qihong , Zhang, Xiaorui , Ou, Fangping et al. Detection and characterization of heteroresistance to chloramphenicol in Klebsiella pneumoniae isolates [J]. | BMC MICROBIOLOGY , 2025 , 25 (1) . |
| MLA | Kuang, Qihong et al. "Detection and characterization of heteroresistance to chloramphenicol in Klebsiella pneumoniae isolates" . | BMC MICROBIOLOGY 25 . 1 (2025) . |
| APA | Kuang, Qihong , Zhang, Xiaorui , Ou, Fangping , Liu, Lingling , Deng, Hui , Yang, Bo et al. Detection and characterization of heteroresistance to chloramphenicol in Klebsiella pneumoniae isolates . | BMC MICROBIOLOGY , 2025 , 25 (1) . |
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【目的】通过生物信息学方法筛选小鹅瘟（gosling plague, GP）相关数据集的致病核心基因及主要信号通路，预测潜在治疗靶点和有效干预中药。【方法】通过收集GeneCards数据库中GP相关靶点并经Uniprot数据库标准化，提取基因表达综合数据库（gene expression omnibus, GEO）肠道炎症数据集（GSE14841）和营养不良数据集（GSE43698），合并使用R语言Limmar包筛选GP的差异表达基因（differentially expressed genes, DEGs）。利用DAVID数据库对DEGs进行基因本体论（GeneOntology,GO）分析以及京都基因与基因组百科全书（Kyoto EncyclopediaofGenesandGenomes,KEGG）富集分析，通过STRING数据库构建蛋白互作网络（protein-protein interaction network, PPI）,Cytoscape软件及其插件筛选子网络核心基因。将核心基因与Coremine Medical数据库相互映射，筛选能够治疗GP的潜在中药。【结果】共筛选得到58个DEGs。富集分析结果显示，DEGs主要参与宿主细胞膜受体识别病毒蛋白、细胞质水解酶与转移酶活性等生物过程，定位于肌动蛋白细胞骨架（actin cytoskeleton,AC），环鸟苷酸-腺苷酸合成酶和干扰素基因刺激因子（cytosolic DNA-Sensing and the STING, cGAS-STING）、丝裂原活化蛋白激酶（mitogen-activated protein kinase, MARK）与Toll样受体（toll-like receptor, TLR）信号通路。通过PPI鉴定出Degree值前10名关键基因：干扰素诱导螺旋酶C结构域3(interferon induced with helicase C domain 3,IFIH3)、干扰素诱导螺旋酶C结构域1(interferon induced with helicase C domain 1, IFIH1)、线粒体抗病毒信号蛋白（mitochondrial antiviral signaling protein, MAVS）、C-C趋化因子配体5(C-C motif chemokine ligand 5, CCL5)、Toll样受体4(tol...
Keyword ：
中药预测 中药预测 小鹅瘟 小鹅瘟 生物信息学 生物信息学 虚拟筛选 虚拟筛选 鹅细小病毒 鹅细小病毒
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| GB/T 7714 | 王劭 , 孙雪岩 , 张烨淳 et al. 基于生物信息学筛选小鹅瘟差异表达基因及潜在治疗中药预测 [J]. | 福建农业学报 , 2025 , 40 (03) : 253-261 . |
| MLA | 王劭 et al. "基于生物信息学筛选小鹅瘟差异表达基因及潜在治疗中药预测" . | 福建农业学报 40 . 03 (2025) : 253-261 . |
| APA | 王劭 , 孙雪岩 , 张烨淳 , 袁孝武 , 徐见光 , 邝启红 et al. 基于生物信息学筛选小鹅瘟差异表达基因及潜在治疗中药预测 . | 福建农业学报 , 2025 , 40 (03) , 253-261 . |
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为快速检测猪捷申病毒(PTV),根据PTV 5′UTR基因保守序列设计特异性引物和探针，优化荧光逆转录重组酶介导等温扩增(RT-RAA)反应条件和体系，并通过特异性、敏感性、重复性试验和临床应用对RT-RAA进行评价。结果表明，在37℃恒温反应22 min即可完成对PTV核酸扩增，最低检出限度为30.1 copies/μL;与猪流行性腹泻病毒(PEDV)、猪传染性胃肠炎病毒(TGEV)、猪轮状病毒(PoRV)、猪德尔塔冠状病毒(PDCoV)均无交叉反应；重复性试验显示，组内和组间变异系数均小于5%;23份临床样品检测显示PTV阳性率为21.74%(5/23),检测结果与RT-qPCR一致。说明建立的PTV荧光RT-RAA检测方法具有简便、高效、稳定等优点，为PTV的快速检测和流行病学调查提供了技术手段。
Keyword ：
检测方法 检测方法 猪捷申病毒 猪捷申病毒 荧光逆转录重组酶介导等温扩增 荧光逆转录重组酶介导等温扩增
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| GB/T 7714 | 孙志华 , 林青 , 康龙滨 et al. 猪捷申病毒荧光RT-RAA检测方法的建立及应用 [J]. | 动物医学进展 , 2025 , 46 (03) : 130-133 . |
| MLA | 孙志华 et al. "猪捷申病毒荧光RT-RAA检测方法的建立及应用" . | 动物医学进展 46 . 03 (2025) : 130-133 . |
| APA | 孙志华 , 林青 , 康龙滨 , 王隆柏 , 周伦江 , 俞道进 et al. 猪捷申病毒荧光RT-RAA检测方法的建立及应用 . | 动物医学进展 , 2025 , 46 (03) , 130-133 . |
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<正>0引言后非洲猪瘟时代猪蓝耳病流行趋势及防控思路发生较大变化，最为重要的转变是猪场普遍建立起良好的针对人车物料的生物安全体系，外源性输入较为彻底的切断成为许多猪场构建阴性群体的重要基础。当然，后备猪和精液的猪蓝耳病病原输入仍然时有发生。全进全出，批次化生产以及两点式饲养等模式下猪蓝耳病的影响相对较小提供了更好的关于猪蓝耳病防控的思维。实际上，越来越多人认识到直接的接触传播是猪蓝耳病传播的主要途径，在中国南方，气溶胶传播被认为不是特别重要的传播方式，福建省目前众多的猪蓝耳病阴性场全部没有使用空气过滤系统。
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| GB/T 7714 | 俞道进 . 保育猪群猪蓝耳病快速稳定技术 [J]. | 猪业科学 , 2025 , 42 (01) : 34-35,6 . |
| MLA | 俞道进 . "保育猪群猪蓝耳病快速稳定技术" . | 猪业科学 42 . 01 (2025) : 34-35,6 . |
| APA | 俞道进 . 保育猪群猪蓝耳病快速稳定技术 . | 猪业科学 , 2025 , 42 (01) , 34-35,6 . |
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Background: The impact of heat stress on intestinal bacterial antimicrobial resistance (AMR) and its underlying mechanisms is not fully understood. This study aims to explore how heat stress influences AMR in the gut and the mechanisms involved. Methods: A Specific-Pathogen-Free (SPF) mouse model was used, divided into a control group (maintained at 25 degrees C) and a heat stress group (exposed to 42 degrees C for 30 min twice daily for 55 days). The effectiveness of the model was verified by RT-qPCR and histopathological analysis. Antibiotic susceptibility testing and clonal analysis (ERIC-PCR) were performed. Colonization assays were conducted to determine the accumulation of resistant strains in the gut. Metagenomic sequencing was conducted to investigated microbial composition. Results: RT-qPCR and Histopathological analysis revealed intestinal damage and significant upregulation of genes related to stress response, intestinal barrier integrity and inflammation, indicating successful model establishment and physiological alterations. Antibiotic susceptibility testing revealed increased resistance to erythromycin, chloramphenicol, and tetracycline among Enterococcus strains. Clonal analysis demonstrated that these resistant strains were clonally unrelated. Sequencing identified a novel ermB-carrying integrative and conjugative element (ICEFZMF) among four erythromycin-resistant strains. The rectum harbored a higher proportion of erythromycin-resistant Enterococcus strains with elevated minimum inhibitory concentrations (MICs) after 25 days of heat stress exposure. Colonization assays confirmed that heat stress led to the accumulation of erythromycin-resistant Enterococcus in the rectum. Metagenomic sequencing revealed significant changes in microbial composition, favoring anaerobic metabolism. Conclusions: This study suggests that chronic heat stress can promote the emergence of antibiotic-resistant strains through ICE transfer, providing insight for environmental safety.
Keyword ：
antimicrobial resistance antimicrobial resistance Enterococcus isolates Enterococcus isolates heat stress heat stress mobile genetic elements mobile genetic elements one health one health
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| GB/T 7714 | Yi, Lingxian , Ren, Zining , Feng, Yu et al. Chronic Heat Stress Can Induce Conjugation of a Novel ermB-Containing ICEFZMF, Increasing Resistance to Erythromycin Among Enterococcus Strains in Diverse Intestinal Segments in the Mouse Model [J]. | ANTIBIOTICS-BASEL , 2025 , 14 (5) . |
| MLA | Yi, Lingxian et al. "Chronic Heat Stress Can Induce Conjugation of a Novel ermB-Containing ICEFZMF, Increasing Resistance to Erythromycin Among Enterococcus Strains in Diverse Intestinal Segments in the Mouse Model" . | ANTIBIOTICS-BASEL 14 . 5 (2025) . |
| APA | Yi, Lingxian , Ren, Zining , Feng, Yu , Zhang, Yechun , Liu, Jianshuo , Yuan, Xiaowu et al. Chronic Heat Stress Can Induce Conjugation of a Novel ermB-Containing ICEFZMF, Increasing Resistance to Erythromycin Among Enterococcus Strains in Diverse Intestinal Segments in the Mouse Model . | ANTIBIOTICS-BASEL , 2025 , 14 (5) . |
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Introduction Matrine (MT) has been found to restore the susceptibility of Escherichia coli to a variety of antibiotics in vitro. Nevertheless, the absence of pharmacokinetic data makes it uncertain whether MT exhibits efficacy in vivo. The study aimed to investigate the kinetic behavior of MT in pig intestinal lumen, the primary site for the colonization of enterotoxigenic Escherichia coli, and to develop a minimal physiologically based pharmacokinetic (PBPK) model for MT in pig intestinal lumen.Methods Two animal experiments were carried out for these purposes. In experiment 1, 12 pigs were implanted with a sterile T-cannula, and then were given a single oral dose of MT or MT-Amoxicillin (AMO) combination at 40 or 70 mg/kg. In experiment 2, 25 pigs were administered with MT at 50 mg/kg/d by oral gavage for 5 d. Intestinal contents were collected at predetermined times and analysed by liquid chromatography tandem mass spectrometry (LC-MS/MS) method. The concentration-time data were analysed by non-compartmental method. Subsequently, a four-compartment PBPK model was developed and validated.Results After oral administrations, the MT concentrations in pig intestinal lumen increased rapidly and reached their peaks within 2 h, then decreased in a two-phase decay pattern. The co-administered AMO did not alter the kinetic behavior of MT in pig intestinal lumen. The PBPK model gave an accurate prediction of MT concentrations in pig intestinal lumen at most time points.Discussion A dosage regimen of 70 mg/kg every 8 h was recommended to ensure a sufficient drug exposure.
Keyword ：
liquid chromatography tandem mass spectrometry liquid chromatography tandem mass spectrometry matrine matrine pharmacodynamic evaluation pharmacodynamic evaluation physiologically based pharmacokinetic model physiologically based pharmacokinetic model pig intestinal lumen pig intestinal lumen
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| GB/T 7714 | Yang, Bo , Jia, YiWei , Wang, FuHao et al. The kinetic behavior of matrine in pig intestinal lumen after oral administration and its physiologically based pharmacokinetic modeling [J]. | FRONTIERS IN VETERINARY SCIENCE , 2025 , 12 . |
| MLA | Yang, Bo et al. "The kinetic behavior of matrine in pig intestinal lumen after oral administration and its physiologically based pharmacokinetic modeling" . | FRONTIERS IN VETERINARY SCIENCE 12 (2025) . |
| APA | Yang, Bo , Jia, YiWei , Wang, FuHao , Lv, XiaoLing , Ma, SuYang , Tan, YaXin et al. The kinetic behavior of matrine in pig intestinal lumen after oral administration and its physiologically based pharmacokinetic modeling . | FRONTIERS IN VETERINARY SCIENCE , 2025 , 12 . |
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