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学者姓名：吴异健
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Abstract ：
Mycoplasma synoviae (MS) infection can cause severe inflammatory responses in avian species, evading immune recognition, leading to chronic infection and immune dysfunction. Although the pathogenic mechanisms and immune evasion strategies of MS have not been fully elucidated, the crucial role of the spleen in immune responses cannot be overlooked. Building on preliminary findings related to the tissue damage and inflammatory responses in chicken spleens caused by MS infection, this study further utilizes transcriptomic and proteomic technologies to thoroughly investigate the comprehensive effects of MS infection on the chicken spleen. We detected 946 differentially expressed genes (DEGs) and 305 differentially expressed proteins (DEPs) during MS infection, including 771 up-regulated and 175 down-regulated DEGs, along with 145 up-regulated and 160 down-regulated DEPs. Gene Ontology (GO) analysis indicated that the DEGs/DEPs are enriched in processes related to immune activation, antioxidation/cell apoptosis, inflammation, and endoplasmic reticulum protein processing. KEGG pathway enrichment analysis revealed immune-related pathways closely associated with MS infection, particularly in endoplasmic reticulum protein processing, cytokine-cytokine receptor interaction, and the myosin light chain kinase (MAPK) signalling pathway. Suggesting that the activation and interaction of these pathways may play a critical role in the immune response during MS infection. Overall, this study, for the first time, employs RNA-Seq and TMT quantitative proteomics to comprehensively analyse the interaction mechanisms between MS and chicken spleen, enhancing our understanding of the complexity of the immune mechanisms induced by MS. This provides valuable insights for future research and intervention strategies targeting MS infection.
Keyword ：
immune responses immune responses Mycoplasma synoviae Mycoplasma synoviae proteomic analysis proteomic analysis spleen spleen transcriptomic analysis transcriptomic analysis
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| GB/T 7714 | Zhong, Lemiao , Wu, Chunlin , Liao, Lvyan et al. Comparative omics analysis of the impact of Mycoplasma synoviae infection on the immune mechanisms of poultry spleen [J]. | VIRULENCE , 2025 , 16 (1) . |
| MLA | Zhong, Lemiao et al. "Comparative omics analysis of the impact of Mycoplasma synoviae infection on the immune mechanisms of poultry spleen" . | VIRULENCE 16 . 1 (2025) . |
| APA | Zhong, Lemiao , Wu, Chunlin , Liao, Lvyan , Li, Jian , Wu, Yijian . Comparative omics analysis of the impact of Mycoplasma synoviae infection on the immune mechanisms of poultry spleen . | VIRULENCE , 2025 , 16 (1) . |
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冠状病毒(CoVs)因其对人类和动物健康造成的严重威胁而广受关注。凝集素格瑞弗森(GRFT)在冠状病毒感染防控中显示出优良的抗病毒作用，已成为研究热点。论文重点剖析了冠状病毒囊膜蛋白的糖基化修饰特征、GRFT结构及其抗病毒机制，总结了GRFT抗人类和动物冠状病毒的研究进展，并分析GRFT作为潜在抗冠状病毒药物的优势与面临的挑战。未来可通过多种抗病毒成分联合应用和优化GRFT递送系统，进一步提升其在人类临床和兽医领域中的应用潜力。此外，结合基因编辑技术，有望降低GRFT生产成本并提高其治疗效果，为防控人类和动物冠状病毒性疫病提供新的策略。
Keyword ：
冠状病毒 冠状病毒 刺突蛋白 刺突蛋白 抗病毒感染 抗病毒感染 格瑞弗森 格瑞弗森 糖基化修饰 糖基化修饰
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| GB/T 7714 | 梅朋 , 黄晓紫 , 周炫汝 et al. 凝集素Griffithsin抗冠状病毒作用的研究进展 [J]. | 动物医学进展 , 2025 , 46 (06) : 97-104 . |
| MLA | 梅朋 et al. "凝集素Griffithsin抗冠状病毒作用的研究进展" . | 动物医学进展 46 . 06 (2025) : 97-104 . |
| APA | 梅朋 , 黄晓紫 , 周炫汝 , 崔红杰 , 吴异健 , 刘萍 . 凝集素Griffithsin抗冠状病毒作用的研究进展 . | 动物医学进展 , 2025 , 46 (06) , 97-104 . |
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为探究抗氧化衍生物Q-Griffithsin(Q-GRFT)对猪流行性腹泻病毒（PEDV）的抑制作用，构建重组表达质粒pGEX-4T-Q-GRFT，并在原核表达体系中表达重组蛋白Q-GRFT。随后利用镍离子亲和层析技术纯化目标蛋白，并验证其浓缩后蛋白的特异性。通过体外试验进一步证实其抗PEDV作用。结果表明，pGEX-4T-Q-GRFT转化至感受态细胞中，在22℃下诱导14 h后，表达出与预期大小一致的Q-GRFT蛋白，纯化效率为3.01 mg/L。在细胞水平上，Q-GRFT显著减轻了PEDV感染引发的细胞病变，抑制了PEDV在细胞中的复制，且其抗病毒效果呈质量浓度依赖性。综上所述，成功制备了具有抗PEDV活性的重组蛋白Q-GRFT，为抗动物冠状病毒药物的研发提供了理论依据，并为PEDV的防治提供了新的策略。
Keyword ：
Q-Griffithsin Q-Griffithsin 抗病毒活性 抗病毒活性 猪流行性腹泻病毒 猪流行性腹泻病毒 蛋白表达 蛋白表达
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| GB/T 7714 | 周炫汝 , 刘子岚 , 林起鑫 et al. 凝集素Q-Griffithsin的克隆表达及其对猪流行性腹泻病毒的抑制作用 [J]. | 中国兽医科学 , 2025 , 55 (05) : 632-639 . |
| MLA | 周炫汝 et al. "凝集素Q-Griffithsin的克隆表达及其对猪流行性腹泻病毒的抑制作用" . | 中国兽医科学 55 . 05 (2025) : 632-639 . |
| APA | 周炫汝 , 刘子岚 , 林起鑫 , 梅朋 , 蔡淑铭 , 姜文亿 et al. 凝集素Q-Griffithsin的克隆表达及其对猪流行性腹泻病毒的抑制作用 . | 中国兽医科学 , 2025 , 55 (05) , 632-639 . |
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This study was intended to investigate whether Hericium erinaceus polysaccharides (HEP) prevent oxidative stress and apoptosis of intestinal porcine epithelial cells from jejunum (IPEC-J2 cells) induced by hydrogen peroxide (H 2 O 2 ). Crude HEP were extracted and purified by chromatography. The ultraviolet and infrared spectra and monosaccharide composition of HEP were analyzed. Reactive oxygen species (ROS) generation was quantified by flow cytometry method, and lactate dehydrogenase (LDH) and malondialdehyde (MDA) production were determined by TBARS. Also, apoptosis was analyzed by flow cytometry method and the apoptosis-related regulatory molecules were determined by microplate or western blotting method. Our results showed that pretreatment of IPEC-J2 cells with HEP significantly scavenged ROS and reduced LDH and MDA production. HEP also reduced apoptosis and kept polarity of the mitochondrial membrane potential. Moreover, HEP increased the content of caspase-3 and PARP, and protein expression of Bcl-2, while inhibited Bax and Bad and reduced the content of caspase-9 and release of Cyt - C. Meanwhile, HEP inhibited the protein expression of TNFR1, FAS, and FASL, and decreased the content of caspase-8. The results indicated that HEP had a protective effect against oxidative stress in IPEC-J2 cells and the underlying mechanism was reducing apoptosis via mitochondrial and death receptor pathways.
Keyword ：
Apoptosis Apoptosis Hericium erinaceus polysaccharides Hericium erinaceus polysaccharides Hydrogen peroxide Hydrogen peroxide IPEC-J2 cells IPEC-J2 cells Oxidative stress Oxidative stress
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| GB/T 7714 | Li, Jian , Mo, Jia-rong , Hu, Shi-yu et al. Effects of Hericium erinaceus polysaccharide in porcine IPEC-J2 intestinal epithelial cells against apoptosis induced by oxidative stress [J]. | COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY , 2024 , 280 . |
| MLA | Li, Jian et al. "Effects of Hericium erinaceus polysaccharide in porcine IPEC-J2 intestinal epithelial cells against apoptosis induced by oxidative stress" . | COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY 280 (2024) . |
| APA | Li, Jian , Mo, Jia-rong , Hu, Shi-yu , Dong, Xin , Li, Jia-wei , Yang, Li-yu et al. Effects of Hericium erinaceus polysaccharide in porcine IPEC-J2 intestinal epithelial cells against apoptosis induced by oxidative stress . | COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY , 2024 , 280 . |
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【目的】研究福建南平地区禽腺病毒(FAdV)血清8a型分离株的遗传演化情况及致病性，旨在为防控血清8a型FAdV感染提供参考。【方法】2022年8月，采集福建南平地区部分肉鸡养殖场出现的以包涵体肝炎为特征的肝脏病料，采用PCR技术检测FAdV核酸，将获得的阳性样品接种在无特定病原体(SPF)鸡胚上进行病原的分离纯化，利用hexon基因测序及序列分析等方法进行病毒鉴定，并在测定分离株毒力的基础上进行动物致病性试验。【结果】接种10 d后，鸡胚死亡，肝脏肿大、出血、坏死、呈土黄色；PCR鉴定和hexon基因序列分析表明，分离株与GenBank上的FAdV E型8a血清型澳大利亚TR59毒株的序列同源性高达99.5%,将该分离株命名为FJNP。FJNP株对1日龄SPF鸡的致死率为44%(11/25);剖检显示，感染鸡肝脏肿大、出血、边缘钝圆、呈土黄色、出现白色坏死点，脾脏和肾脏也出现肿大、出血等症状；肝脏组织病理学检查结果显示，感染鸡肝细胞大量变性坏死，肝细胞核可见嗜碱性包涵体及大量淋巴细胞浸润。实时荧光定量PCR检测显示，感染鸡体内多个组织器官均检测到病毒，且主要分布在肝脏，攻毒3、5、7、14、21 d在泄殖腔中均能检测到病毒。【结论】本研究从临床表现为包涵体肝炎的病鸡肝脏病料中分离到一株具有较强致病性的血清8a型FAdV。
Keyword ：
hexon基因 hexon基因 分离鉴定 分离鉴定 致病性 致病性 血清8a型禽腺病毒 血清8a型禽腺病毒
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| GB/T 7714 | 谢涛 , 黄宝钦 , 谢逸天 et al. 一株血清8a型禽腺病毒的分离鉴定及致病性分析 [J]. | 福建农林大学学报(自然科学版) , 2024 , 53 (03) : 364-370 . |
| MLA | 谢涛 et al. "一株血清8a型禽腺病毒的分离鉴定及致病性分析" . | 福建农林大学学报(自然科学版) 53 . 03 (2024) : 364-370 . |
| APA | 谢涛 , 黄宝钦 , 谢逸天 , 罗忠宝 , 吴异健 . 一株血清8a型禽腺病毒的分离鉴定及致病性分析 . | 福建农林大学学报(自然科学版) , 2024 , 53 (03) , 364-370 . |
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Mycoplasma gallisepticum (MG) can induce persistent inflammatory damage to the tracheal mucosa of poultry and cause chronic respiratory diseases in chickens. To further investigate the mechanism of MG-induced injury to the tracheal mucosa, we used chick embryo tracheal organ culture (TOC) as a model to study the invasion and reproduction of MG, the effect of MG on tracheal morphology, and the potential factors that promote MG tissue invasion. The results showed that MG infection significantly damaged the tracheal epithelial structure and weakened tracheal epithelial barrier function; MG also increased the occurrence of bacterial displacement, with a significant (p < 0.05) increase in the bacterial load of the infected TOCs at 5 and 7 days post-infection. In addition, MG significantly (p < 0.05) increased the expression levels of inflammatory cytokines, such as TNF-alpha, interleukin-1 beta (IL-1 beta), and IL-6, and activated the NF-kappa B signalling pathway, leading to increased nuclear translocation of NF-kappa B p65. Simultaneously, the map kinase pathway (MAPK) was activated. This activation might be associated with increased myosin light chain (MLC) phosphorylation, which could lead to actin-myosin contraction and disruption of tight junction (TJ) protein function, potentially compromising epithelial barrier integrity and further catalysing MG migration into tissues. Overall, our results contribute to a better understanding of the interaction between MG and the host, provide insight into the mechanisms of damage to the tracheal mucosa induced by MG infection, and provide new insights into the possible pathways involved in Mycoplasma gallisepticum infection in vivo.
Keyword ：
chicken embryo tracheal organ culture chicken embryo tracheal organ culture inflammatory cytokines inflammatory cytokines Mycoplasma gallisepticum Mycoplasma gallisepticum NF-kappa B/MLCK pathway NF-kappa B/MLCK pathway TJ protein TJ protein tracheal epithelial barrier tracheal epithelial barrier
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| GB/T 7714 | Zhong, Lemiao , Wu, Chunlin , Zhao, Yan et al. Inflammatory responses and barrier disruption in the trachea of chicks following Mycoplasma gallisepticum infection: a focus on the TNF-α-NF-κB/MLCK pathway [J]. | VETERINARY RESEARCH , 2024 , 55 (1) . |
| MLA | Zhong, Lemiao et al. "Inflammatory responses and barrier disruption in the trachea of chicks following Mycoplasma gallisepticum infection: a focus on the TNF-α-NF-κB/MLCK pathway" . | VETERINARY RESEARCH 55 . 1 (2024) . |
| APA | Zhong, Lemiao , Wu, Chunlin , Zhao, Yan , Huang, Baoqin , Luo, Zhongbao , Wu, Yijian . Inflammatory responses and barrier disruption in the trachea of chicks following Mycoplasma gallisepticum infection: a focus on the TNF-α-NF-κB/MLCK pathway . | VETERINARY RESEARCH , 2024 , 55 (1) . |
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Mycoplasma synovium (MS) is a prominent avian pathogen known to elicit robust inflammatory responses in birds while evading immune detection, often leading to chronic infection and immune compromise. The mechanisms underpinning MS-mediated splenic tissue damage in chickens, however, remain undefined. In our investigation with 7-day-old SPF chickens, we administered an MS-Y bacterial solution (200 mu l, 1 x 10(9) CCU/ml) through eye and nose droplets, collecting spleen samples on days 3, 6, and 12 post-infection. Comprehensive analyses utilizing histopathology, electron microscopy, TUNEL assay, qRT-PCR, and western blot were employed. Results demonstrated that MS-infection downregulated T-SOD, GSH-PX, and CAT, while concurrently elevating iNOS, NO, and MDA levels. Evidently, MS-induced oxidative stress compromised the spleen's antioxidant defences. Histological examinations pinpointed splenic damage characterized by lymphocyte reduction and increased inflammatory cell infiltration. Ultrastructural observations revealed clear apoptotic markers, including mitochondrial perturbations and nuclear anomalies. Importantly, MS induced significant spleen tissue apoptosis, as supported by TUNEL assay outputs and gene expression profiles associated with apoptosis. Concurrently, we observed upregulated expressions of mRNAs and proteins affiliated with the NF-kappa B/MAPK signalling cascade (p < 0.05). Collectively, our data elucidate that MS infection induces splenic apoptosis and oxidative disturbances, perturbs tissue integrity, and potentiates the NF-kappa B/MAPK-mediated inflammatory cascade.
Keyword ：
apoptosis apoptosis inflammation inflammation Mycoplasma synovium Mycoplasma synovium oxidative stress oxidative stress spleen spleen
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| GB/T 7714 | Zhong, Lemiao , Wu, Chunlin , Liao, Lvyan et al. Mycoplasma synoviae induce spleen tissue damage and inflammatory response of chicken through oxidative stress and apoptosis [J]. | VIRULENCE , 2024 , 15 (1) . |
| MLA | Zhong, Lemiao et al. "Mycoplasma synoviae induce spleen tissue damage and inflammatory response of chicken through oxidative stress and apoptosis" . | VIRULENCE 15 . 1 (2024) . |
| APA | Zhong, Lemiao , Wu, Chunlin , Liao, Lvyan , Wu, Yijian . Mycoplasma synoviae induce spleen tissue damage and inflammatory response of chicken through oxidative stress and apoptosis . | VIRULENCE , 2024 , 15 (1) . |
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为了建立一种快速准确鉴别滑液囊支原体MS-Y分离株和活疫苗MS-H的方法，根据滑液囊支原体ppm和deoD基因的2个碱基突变位点序列，运用错配扩增突变分析（MAMA）PCR方法，设计了2对特异性引物和1对通用引物，用于扩增目的基因，通过优化反应条件，建立一种能同时鉴别出MS-Y和MS-H的多重PCR检测方法，并检测了该方法的特异性和敏感性。结果显示，利用特异性引物的多重PCR方法能同时鉴别滑液囊支原体MS-H活疫苗株和滑液囊支原体MS-Y分离株的DNA，分别扩增出相应的特异目的条带，检测MS-H株的最低敏感度为4.21×10~5 copies/μL，检测MS-Y株的最低敏感度为6.47×10~5 copies/μL；而利用通用引物对检测滑液囊支原体DNA的最低浓度为2.18×10~3 copies/μL，检测MS-H DNA的浓度下限均为1×10~5 CCU/mL，检测MS-Y DNA的最低浓度为1×10~3 CCU/mL。本研究建立了一种能同时快速鉴别滑液囊支原体MS-H活疫苗株和MS-Y分离株的多重PCR方法。
Keyword ：
MS-H MS-H 多重PCR 多重PCR 滑液囊支原体 滑液囊支原体
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| GB/T 7714 | 张立根 , 李娜 , 赵妍 et al. 鉴别滑液囊支原体MS-Y田间株与MS-H疫苗株多重PCR方法的建立 [J]. | 中国兽医科学 , 2023 , 53 (01) : 34-42 . |
| MLA | 张立根 et al. "鉴别滑液囊支原体MS-Y田间株与MS-H疫苗株多重PCR方法的建立" . | 中国兽医科学 53 . 01 (2023) : 34-42 . |
| APA | 张立根 , 李娜 , 赵妍 , 黄宝钦 , 罗忠宝 , 吴异健 . 鉴别滑液囊支原体MS-Y田间株与MS-H疫苗株多重PCR方法的建立 . | 中国兽医科学 , 2023 , 53 (01) , 34-42 . |
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本发明提供了一种鸡滑液囊支原体多抗原表位蛋白及其应用。所述鸡滑液囊支原体多抗原表位蛋白的氨基酸序列如SEQ ID NO.1所示，编码该鸡滑液囊支原体多抗原表位蛋白的基因的核苷酸序列如SEQ ID NO.2所示。所述鸡滑液囊支原体多抗原表位蛋白经过纯化和鉴定后通过SPF雏鸡进行的免疫攻毒保护试验，初步显示出了良好的免疫保护效果。本研究为鸡滑液囊支原体基因工程疫苗的研发奠定基础。
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| GB/T 7714 | 吴异健 , 钟乐苗 , 廖吕燕 et al. 一种鸡滑液囊支原体多抗原表位蛋白及其应用 : CN202310623003.X[P]. | 2023-05-30 . |
| MLA | 吴异健 et al. "一种鸡滑液囊支原体多抗原表位蛋白及其应用" : CN202310623003.X. | 2023-05-30 . |
| APA | 吴异健 , 钟乐苗 , 廖吕燕 , 张立根 . 一种鸡滑液囊支原体多抗原表位蛋白及其应用 : CN202310623003.X. | 2023-05-30 . |
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本发明公开了白碘酚在制备蛋鸡输卵管炎造模剂中的应用，白碘酚为含碘药物、白芨、甘油和纯水的混合物，一种蛋鸡输卵管炎造模方法，通过输卵管灌注法进行蛋鸡输卵管炎造模，包括以下步骤：步骤一、配制白碘酚；步骤二、将配置好的试剂存储于锥形瓶中，做好密封保存，备用；步骤三、开始造模前，每天进行早晚两次饲喂，直到蛋鸡的产蛋率能够稳定在90％以上，之后开始进行造模。本发明的有益效果为：将自制的造模制剂，通过输精管灌注到蛋鸡输卵管子宫部的方法，成功制造的单机输卵管炎的病理模型；具有操作简单、可重复性好、炎性特征明显等优点，可为蛋鸡输卵管炎的相关研究提供试验手段。
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| GB/T 7714 | 李健 , 李荣强 , 赵永兵 et al. 白碘酚在制备蛋鸡输卵管炎造模剂中的应用及其造模方法 : CN202310742284.0[P]. | 2023-06-21 . |
| MLA | 李健 et al. "白碘酚在制备蛋鸡输卵管炎造模剂中的应用及其造模方法" : CN202310742284.0. | 2023-06-21 . |
| APA | 李健 , 李荣强 , 赵永兵 , 白丁平 , 秦韬 , 吴异健 et al. 白碘酚在制备蛋鸡输卵管炎造模剂中的应用及其造模方法 : CN202310742284.0. | 2023-06-21 . |
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