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学者姓名：连森阳
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Abstract ：
试验旨在探索白羽番鸭的产蛋规律。运用伍德、分室和杨宁模型以及Logistic、Gompertz和Von Bertalanffy模型分别拟合27～48周龄的1 114羽白羽番鸭的产蛋率和累计产蛋量曲线，并分析比较实际观测值和各模型预测值。结果显示：（1）分室和杨宁模型2种产蛋率曲线模型拟合优度均高于0.830，杨宁模型拟合效果最好，而伍德模型的拟合效果最差，其R~2仅为0.4672。（2）3种累计产蛋量曲线模型拟合优度均高于0.990，其中Von Bertalanffy模型的R~2高达0.9985，拟合效果最佳。因此，杨宁模型和Von Bertalanffy模型分别是拟合白羽番鸭产蛋率和累计产蛋量曲线的最佳数学模型。
Keyword ：
产蛋率 产蛋率 曲线拟合 曲线拟合 白羽番鸭 白羽番鸭 累计产蛋数 累计产蛋数
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| GB/T 7714 | 徐丽 , 梅慧灵 , 吴旭 et al. 白羽番鸭产蛋率和累计产蛋量的曲线拟合分析 [J]. | 福建畜牧兽医 , 2024 , 46 (05) : 4-8 . |
| MLA | 徐丽 et al. "白羽番鸭产蛋率和累计产蛋量的曲线拟合分析" . | 福建畜牧兽医 46 . 05 (2024) : 4-8 . |
| APA | 徐丽 , 梅慧灵 , 吴旭 , 连森阳 , 李昂 , 朱志明 et al. 白羽番鸭产蛋率和累计产蛋量的曲线拟合分析 . | 福建畜牧兽医 , 2024 , 46 (05) , 4-8 . |
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本试验旨在研究番鸭正常蛋、沙壳蛋和条纹蛋等3种不同质地蛋壳在超微结构性状上的异同，并分析蛋壳超微结构性状与蛋壳强度之间的关联性。试验选用300日龄笼养白羽番鸭所产白色的正常蛋、沙壳蛋和条纹蛋厚度相近的蛋壳各12枚，分别作为3种不同质地蛋壳的试验组，每组设12个重复，每个重复1枚蛋壳。使用S3400N型扫描电镜观察蛋壳的超微结构，测定乳突层厚度和乳突间隙，计算有效厚度和平均乳突大小。结果表明：1)蛋壳强度在3种蛋之间差异显著(P<0.05)，表现为正常蛋>沙壳蛋>条纹蛋；3种蛋间蛋形指数和钙化壳重差异均不显著(P>0.05)。2)在超微结构上，沙壳蛋和条纹蛋蛋壳的外表面皲裂较正常蛋蛋壳多而深，气孔较多，表观不平整；与正常蛋蛋壳相比，沙壳蛋蛋壳内表面纤维疏松凌乱，纤维间空隙较多，而条纹蛋蛋壳内表面纤维分布较沙壳蛋更为稀少，纤维间有明显间隙；沙壳蛋和条纹蛋蛋壳的横断面平整性和致密度都更差，乳突层与壳膜层联系也更加不紧密。在超微结构性状中，3种蛋壳间的钙化壳厚度和平均乳突大小差异不显著(P>0.05)；沙壳蛋和条纹蛋蛋壳的乳突间隙均显著大于正常蛋蛋壳(P<0.05)，而沙壳蛋和条纹蛋蛋壳之间差异不显著(P>0.05)；乳突层厚度在3种蛋壳之间均存在显著差异(P<0.05)，表现为正常蛋<沙壳蛋<条纹蛋；3种蛋壳间有效厚度也存在显著差异(P<0.05)，但表现为正常蛋>沙壳蛋>条纹蛋。3)3种蛋壳的乳突层厚度与蛋壳强度呈显著负相关(P<0.05)，其相关系数分别为-0.868、-0.856和-0.837；乳突间隙与蛋壳强度也呈显著负相关(P<0.05)，其相关系数分别为-0.736、-0.764和-0.781；有效厚度与蛋壳强度呈显著正相关(P<0.05)，其相关系数分别为0.693、0.743和0.657；钙化壳厚度和平均乳突大小与蛋壳强度的相关性均不显著(P>0.05)。由此可见，厚度相似的番鸭正常蛋、沙壳蛋和条纹蛋蛋壳其蛋壳强度和超微结构均存在显著差异，其中乳突层厚度和乳突间隙与蛋壳强度均呈显著负相关，有效厚度与蛋壳强度呈显著正相关；在超微结构层面上，乳突层厚度、乳突间隙及有效厚度是影响蛋壳强度的主要因素。
Keyword ：
条纹蛋 条纹蛋 正常蛋 正常蛋 沙壳蛋 沙壳蛋 番鸭 番鸭 蛋壳强度 蛋壳强度 超微结构 超微结构
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| GB/T 7714 | 梅慧灵 , 郑定黔 , 赵邦哲 et al. 番鸭不同质地蛋壳超微结构及其与蛋壳强度的关联分析 [J]. | 动物营养学报 , 2024 , 36 (02) : 1350-1360 . |
| MLA | 梅慧灵 et al. "番鸭不同质地蛋壳超微结构及其与蛋壳强度的关联分析" . | 动物营养学报 36 . 02 (2024) : 1350-1360 . |
| APA | 梅慧灵 , 郑定黔 , 赵邦哲 , 田若宁 , 陈莹 , 李昂 et al. 番鸭不同质地蛋壳超微结构及其与蛋壳强度的关联分析 . | 动物营养学报 , 2024 , 36 (02) , 1350-1360 . |
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Abstract ：
本试验旨在研究蛋壳元素组成、超微结构和钙化壳基质含量对龙岩山麻鸭蛋壳质量的影响。试验选用300日龄龙岩山麻鸭所产白壳蛋300个，根据蛋壳强度平均分为高、中、低3个试验组，每组5个重复，每个重复20个蛋，测定蛋壳质量、元素组成和超微结构。结果表明：高、中、低蛋壳强度组蛋壳强度分别为（4.67±0.09） kg/cm2、（3.78±0.02） kg/cm2和（2.86±0.03） kg/cm2，组间差异显著（P<0.05）；各组间的蛋重、蛋形指数、蛋壳厚和蛋壳重无显著差异（P>0.05）。3组间钙化壳的基质含量差异不显著（P>0.05），各组元素相对含量在蛋壳超微结构的不同层次差异不显著（P>0.05）。对蛋壳超微结构观察发现，与中、低强度蛋壳组相比，高强度蛋壳组横断面致密性更好，乳突顶部连接更紧密；并且各组间的乳突层有效厚度差异显著（P<0.05），高、低蛋壳强度组的乳突间隙差异显著（P<0.05）。综上所述，厚度相似的蛋壳，其蛋壳各层元素相对含量和基质含量对龙岩山麻鸭蛋壳强度无显著影响，蛋壳超微结构致密度对蛋壳质量具有重要作用，其中乳突层有效厚度和乳突间隙是影响蛋壳质量的重要因素。
Keyword ：
元素组成 元素组成 基质含量 基质含量 蛋壳强度 蛋壳强度 超微结构 超微结构 龙岩山麻鸭 龙岩山麻鸭
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| GB/T 7714 | 王蕾 , 赵邦哲 , 张迎燕 et al. 龙岩山麻鸭蛋壳质量与元素组成、基质含量和超微结构关系的研究 [J]. | 动物营养学报 , 2024 , 36 (03) : 1665-1673 . |
| MLA | 王蕾 et al. "龙岩山麻鸭蛋壳质量与元素组成、基质含量和超微结构关系的研究" . | 动物营养学报 36 . 03 (2024) : 1665-1673 . |
| APA | 王蕾 , 赵邦哲 , 张迎燕 , 田若宁 , 王雅婷 , 李昂 et al. 龙岩山麻鸭蛋壳质量与元素组成、基质含量和超微结构关系的研究 . | 动物营养学报 , 2024 , 36 (03) , 1665-1673 . |
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In order to analyze the relationship between organic matrix and eggshell properties in Mus-covy duck eggshells with different qualities, the organic matrices in the eggshells of normal, pimpled, and striped eggs of white-feathered Muscovy ducks were extracted and separated into acid-insoluble, water-insoluble, and facultative-soluble matrix (both acid-and water-soluble). There was no significant difference in calcified shell thick-ness between normal and pimpled eggs. However, the per-centages of acid-insoluble matrix and total matrix were significantly higher, and the breaking strength was signifi-cantly lower in pimpled eggs than those in normal eggs. In striped eggs, the percentages of acid-insoluble matrix, fac-ultative-soluble matrix, and total matrix, calcified shell weight, calcified shell thickness, and breaking strength were significantly lower than those in normal eggs. The amount and percentage of 3 organic matrices (water -insol-uble matrix, facultative-soluble matrix, and total matrix) were significantly positively correlated with calcified shell thickness in normal eggs rather than striped and pimpled eggs. Our results also demonstrated that there was no lin-ear correlation between the organic components in the 3 Muscovy duck eggshells and the mechanical properties of the eggshells. The lower breaking strength of pimpled egg-shells might be due to the unbalanced enrichment of cer-tain proteins, whereas the striped eggs might mainly result from thinner calcified shells and poor balance between different sedimentary layers.
Keyword ：
breaking strength breaking strength Muscovy duck Muscovy duck organic matrix organic matrix pimpled egg pimpled egg striped egg striped egg
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| GB/T 7714 | Fu, Keyu , Zheng, Dingqian , Xiao, Zongjie et al. Correlations between organic matrix and eggshell properties of 3 kinds of eggshells in Muscovy duck (Cairina moschata) [J]. | POULTRY SCIENCE , 2023 , 102 (9) . |
| MLA | Fu, Keyu et al. "Correlations between organic matrix and eggshell properties of 3 kinds of eggshells in Muscovy duck (Cairina moschata)" . | POULTRY SCIENCE 102 . 9 (2023) . |
| APA | Fu, Keyu , Zheng, Dingqian , Xiao, Zongjie , Chen, Ying , Wang, Lei , Lian, Senyang et al. Correlations between organic matrix and eggshell properties of 3 kinds of eggshells in Muscovy duck (Cairina moschata) . | POULTRY SCIENCE , 2023 , 102 (9) . |
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There are recent reports of the important functions of long noncoding RNAs (lncRNAs) in female reproductive and ovarian development. Studies in which there was characterization of lncRNAs in the ovaries of laying compared with nesting poultry, however, are limited. In this study, RNA libraries were constructed by obtaining sequencing data of ovarian tissues from laying and nesting Muscovy ducks. In the ovarian tissues of Muscovy ducks, a total of 334 differentially abundant mRNA transcripts (DEGs) and 36 differentially abundant lncRNA transcripts were identified in the nesting period, when compared with during the laying period. These results were subsequently validated by qRT-PCR using nine randomly-selected lncRNAs and six randomly-selected DAMTs. Furthermore, the cis- and trans-regulatory target genes of differentially abundant lncRNA transcripts were identified, and lncRNA-gene interaction networks of 34 differentially abundant lncRNAs and 263 DEGs were constructed. A total of 7601 lncRNAs neighboring 10,542 protein-coding genes were identified and found to be enriched in the Wnt signaling pathway and oocyte meiosis pathways associated with follicular development. Overall, only 11 cis-targets and 57 mRNA-mRNA except trans-targets were involved in the lncRNA-gene interaction networks. Based on the interaction networks, nine DEGs were trans-regulated by differentially abundant lncRNAs and 20 differentially abundant lncRNAs were hypothesized to have important functions in the regulation of broodiness in Muscovy ducks. In this study, a predicted interaction network of differentially abundant lncRNAs and DEGs in Muscovy ducks was constructed for the first time leading to an enhanced understanding of lncRNA and gene interactions regulating broodiness.
Keyword ：
Broodiness Broodiness Interaction network Interaction network Long noncoding RNA Long noncoding RNA Muscovy duck Muscovy duck Target gene Target gene
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| GB/T 7714 | Wu, Xu , Jiang, Lin , Xu, Faqiong et al. Long noncoding RNAs profiling in ovary during laying and nesting in Muscovy ducks (Cairina moschata) [J]. | ANIMAL REPRODUCTION SCIENCE , 2021 , 230 . |
| MLA | Wu, Xu et al. "Long noncoding RNAs profiling in ovary during laying and nesting in Muscovy ducks (Cairina moschata)" . | ANIMAL REPRODUCTION SCIENCE 230 (2021) . |
| APA | Wu, Xu , Jiang, Lin , Xu, Faqiong , Cao, Sumei , Chen, Yuan , Zhang, Yingyan et al. Long noncoding RNAs profiling in ovary during laying and nesting in Muscovy ducks (Cairina moschata) . | ANIMAL REPRODUCTION SCIENCE , 2021 , 230 . |
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Much attention has been paid to the broodiness of the Muscovy duck, but the molecular mechanism of broodiness remains largely unknown. In this study, the ovary tissues of Muscovy ducks during the broody and laying periods were used to investigate differentially expressed proteins (DEPs) by the iTRAQ-based proteomics approach. A total of 335 DEPs were identified, including 139 up-regulated and 196 down-regulated proteins. Six proteins (APOV1, GAL, SAA, GNB5, VLDLR and CDK1) with higher changes in expression were selected, and these proteins are mainly involved in the pathways related to reproductive performance, such as Oocyte meiosis, and PI3K-Akt signaling pathway. Steroid biosynthesis was the most significantly enriched pathway by KEGG pathway enriched analysis. The qRT-PCR analysis was applied to verify the proteomic analysis. Meanwhile, metabolomics analysis found that several important differentially expressed metabolites (DEMs) (7-dehydrodesmosterol, 25-Hydroxyvitamin D3, 7-Dehydrocholesterol, Pregnanolone, Allopregnanolone and estrogen) that were also mainly involved in Steroid biosynthesis, Steroid hormone biosynthesis and Metabolic pathways. Crucially, the changes in the abundance of these metabolites are closely related to the changes in the protein abundance of proteins identified in the same pathway, and it is always the upstream key enzymes that influence the production of downstream metabolites.
Keyword ：
Broodiness Broodiness iTRAQ iTRAQ Metabolomics Metabolomics Muscovy duck Muscovy duck Ovary Ovary Proteomics Proteomics
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| GB/T 7714 | Wu, Xu , Pan, Xiaoli , Cao, Sumei et al. iTRAQ-based quantitative proteomic analysis provides insights into strong broodiness in Muscovy duck (Cairina moschata) combined with metabolomics analysis [J]. | JOURNAL OF PROTEOMICS , 2019 , 204 . |
| MLA | Wu, Xu et al. "iTRAQ-based quantitative proteomic analysis provides insights into strong broodiness in Muscovy duck (Cairina moschata) combined with metabolomics analysis" . | JOURNAL OF PROTEOMICS 204 (2019) . |
| APA | Wu, Xu , Pan, Xiaoli , Cao, Sumei , Xu, Faqiong , Lan, Liming , Zhang, Yingyan et al. iTRAQ-based quantitative proteomic analysis provides insights into strong broodiness in Muscovy duck (Cairina moschata) combined with metabolomics analysis . | JOURNAL OF PROTEOMICS , 2019 , 204 . |
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应用RT-PCR、3′/5′RACE PCR扩增,克隆了全长为893 bp的山麻鸭Osteopontin(OPN)基因序列,GenBank登录号为XM_013102822。运用生物信息学工具进行分析显示:OPN蛋白一级结构中丝氨酸、半胱氨酸、精氨酸、异亮氨酸、谷氨酸所占比例较高,预测分子式为C_(883)H_(1383)N_(253)O_(267)S_(12)。二级结构预测有8.33%的α螺旋,35.56%的β折叠,56.11%的无规则卷曲。基于编码的氨基酸序列所构建的分子进化树表明,与绿头鸭的OPN基因属于同一分支,亲缘关系最近。在mRNA水平上,OPN基因在山麻鸭心、肝、脾、肺、肾、胸肌、胃、下丘脑、垂体、卵巢、漏斗部、膨大部、卵壳腺中均有不同程度的表达,其中在肾和卵壳腺中的表达量极显著高于其他组织(P<0.01),在肝脏、卵巢、漏斗部和膨大部中的表达量也相对较高,在垂体、胃、脾和下丘脑中的表达量较低。
Keyword ：
克隆 克隆 山麻鸭 山麻鸭 生物信息学 生物信息学 组织表达 组织表达 骨桥蛋白基因 骨桥蛋白基因
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| GB/T 7714 | 潘晓丽 , 林如龙 , 王悦名 et al. 山麻鸭OPN基因的克隆、表达及生物信息学分析 [J]. | 中国家禽 , 2019 , 41 (09) : 15-20 . |
| MLA | 潘晓丽 et al. "山麻鸭OPN基因的克隆、表达及生物信息学分析" . | 中国家禽 41 . 09 (2019) : 15-20 . |
| APA | 潘晓丽 , 林如龙 , 王悦名 , 严美姣 , 连森阳 , 李昂 et al. 山麻鸭OPN基因的克隆、表达及生物信息学分析 . | 中国家禽 , 2019 , 41 (09) , 15-20 . |
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The green eggshell color produced by ducks is a threshold trait that can be influenced by various factors, such as hereditary, environment and nutrition. The aim of this study was to investigate the genetic regulation of the formation of eggs with green shells in Youxian ducks. We performed integrative analysis of mRNAs and miRNAs expression profiling in the shell gland samples from ducks by RNA-Seq. We found 124 differentially expressed genes that were associated with various pathways, such as the ATP-binding cassette (ABC) transporter and solute carrier supper family pathways. A total of 31 differentially expressed miRNAs were found between ducks laying green eggs and white eggs. KEGG pathway analysis of the predicted miRNA target genes also indicated the functional characteristics of these miRNAs; they were involved in the ABC transporter pathway and the solute carrier (SLC) supper family. Analysis with qRT-PCR was applied to validate the results of global gene expression, which showed a correlation between results obtained by RNA-seq and RTq-PCR. Moreover, a miRNA-mRNA interaction network was established using correlation analysis of differentially expressed mRNA and miRNA. Compared to ducks that lay white eggs, ducks that lay green eggs include six up-regulated miRNAs that had regulatory effects on 35 down-regulated genes, and seven down-regulated miRNAs which influenced 46 up regulated genes. For example, the ABC transporter pathway could be regulated by expressing gga-miR-144-3p (up-regulated) with ABCG2 (up-regulated) and other miRNAs and genes. This study provides valuable information about mRNA and miRNA regulation in duck shell gland tissues, and provides foundational information for further study on the eggshell color formation and marker-assisted selection for Youxian duck breeding.
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| GB/T 7714 | Xu, Fa Qiong , Li, Ang , Lan, Jing Jing et al. Study of formation of green eggshell color in ducks through global gene expression [J]. | PLOS ONE , 2018 , 13 (1) . |
| MLA | Xu, Fa Qiong et al. "Study of formation of green eggshell color in ducks through global gene expression" . | PLOS ONE 13 . 1 (2018) . |
| APA | Xu, Fa Qiong , Li, Ang , Lan, Jing Jing , Wang, Yue Ming , Yan, Mei Jiao , Lian, Sen Yang et al. Study of formation of green eggshell color in ducks through global gene expression . | PLOS ONE , 2018 , 13 (1) . |
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表皮生长因子(EGF)是表皮上生长家族的重要调节因子之一,可促进细胞增殖分化、调控细胞的衰老以及诱导肿瘤的发生.在动物卵巢卵泡的研究中发现,EGF可以有效地抑制卵泡细胞的凋亡、促进颗粒细胞的增殖和卵母细胞的成熟,因此近年来受到了越来越多的学者的关注.为了进一步认识EGF基因对各级卵泡的调节作用,文章拟从EGF基因的结构、对卵泡发育的作用机理等角度进行阐述,为研究EGF基因参与调控动物卵泡发育过程的作用机制提供参考.
Keyword ：
EGF基因 EGF基因 作用机制 作用机制 分子结构 分子结构 卵母细胞 卵母细胞 卵泡发育 卵泡发育
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| GB/T 7714 | 许发琼 , 胡玉琼 , 郑恒博 et al. EGF基因在动物卵泡发育过程中的研究进展 [J]. | 黑龙江畜牧兽医（上半月） , 2018 , (11) : 46-49 . |
| MLA | 许发琼 et al. "EGF基因在动物卵泡发育过程中的研究进展" . | 黑龙江畜牧兽医（上半月） 11 (2018) : 46-49 . |
| APA | 许发琼 , 胡玉琼 , 郑恒博 , 吴旭 , 连森阳 , 李昂 . EGF基因在动物卵泡发育过程中的研究进展 . | 黑龙江畜牧兽医（上半月） , 2018 , (11) , 46-49 . |
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Abstract ：
表皮生长因子(EGF)是表皮上生长家族的重要调节因子之一,可促进细胞增殖分化、调控细胞的衰老以及诱导肿瘤的发生。在动物卵巢卵泡的研究中发现,EGF可以有效地抑制卵泡细胞的凋亡、促进颗粒细胞的增殖和卵母细胞的成熟,因此近年来受到了越来越多的学者的关注。为了进一步认识EGF基因对各级卵泡的调节作用,文章拟从EGF基因的结构、对卵泡发育的作用机理等角度进行阐述,为研究EGF基因参与调控动物卵泡发育过程的作用机制提供参考。
Keyword ：
EGF基因 EGF基因 作用机制 作用机制 分子结构 分子结构 卵母细胞 卵母细胞 卵泡发育 卵泡发育
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| GB/T 7714 | 许发琼 , 胡玉琼 , 郑恒博 et al. EGF基因在动物卵泡发育过程中的研究进展 [J]. | 黑龙江畜牧兽医 , 2018 , (21) : 46-49 . |
| MLA | 许发琼 et al. "EGF基因在动物卵泡发育过程中的研究进展" . | 黑龙江畜牧兽医 21 (2018) : 46-49 . |
| APA | 许发琼 , 胡玉琼 , 郑恒博 , 吴旭 , 连森阳 , 李昂 . EGF基因在动物卵泡发育过程中的研究进展 . | 黑龙江畜牧兽医 , 2018 , (21) , 46-49 . |
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