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学者姓名：刘昊
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Abstract ：
Phytophthora infestans, the most damaging oomycete pathogen of potato, is specialized to grow sporangiophore through opened stomata for secondary inoculum production. However, it is still unclear which metabolic pathways in potato are manipulated by P. infestans in the guard cell-pathogen interactions to open the stomata. Here microscopic observations and cell biology were used to investigate antagonistic interactions between guard cells and the oomycete pathogen. We observed that the antagonistic interactions started at the very beginning of infection. Stomatal movement is an important part of the immune response of potato to P. infestans infection and this occurs through guard cell death and stomatal closure. We observed that P. infestans appeared to manipulate metabolic processes in guard cells, such as triacylglycerol (TAG) breakdown, starch degradation, H2O2 scavenging, and NO catabolism, which are involved in stomatal movement, to evade these stomatal defense responses. The signal transduction pathway of P. infestans-induced stomatal opening likely starts from H2O2 and NO scavenging, along with TAG breakdown while the subsequent starch degradation reinforces the opening process by strengthening guard cell turgor and opening the stomata to their maximum aperture. These results suggest that stomata are a barrier stopping P. infestans from completing its life cycle, but this host defense system can be bypassed through the manipulation of diverse metabolic pathways that may be induced by P. infestans effector proteins.
Keyword ：
phytophthora infestans phytophthora infestans potato defences potato defences starch degradation starch degradation stomatal immunity stomatal immunity triacylglycerol breakdown triacylglycerol breakdown
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| GB/T 7714 | Yang, Li-Na , Liu, Hao , Wang, Yan-Ping et al. Pathogen-Mediated Stomatal Opening: A Previously Overlooked Pathogenicity Strategy in the Oomycete Pathogen Phytophthora infestans [J]. | FRONTIERS IN PLANT SCIENCE , 2021 , 12 . |
| MLA | Yang, Li-Na et al. "Pathogen-Mediated Stomatal Opening: A Previously Overlooked Pathogenicity Strategy in the Oomycete Pathogen Phytophthora infestans" . | FRONTIERS IN PLANT SCIENCE 12 (2021) . |
| APA | Yang, Li-Na , Liu, Hao , Wang, Yan-Ping , Seematti, Jenifer , Grenville-Briggs, Laura J. J. , Wang, Zonghua et al. Pathogen-Mediated Stomatal Opening: A Previously Overlooked Pathogenicity Strategy in the Oomycete Pathogen Phytophthora infestans . | FRONTIERS IN PLANT SCIENCE , 2021 , 12 . |
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Intrinsic disorder is a common structural characteristic of proteins and a central player in the biochemical processes of species. However, the role of intrinsic disorder in the evolution of plant-pathogen interactions is rarely investigated. Here, we explored the role of intrinsic disorder in the development of the pathogenicity in the RXLR AVR2 effector of Phytophthora infestans. We found AVR2 exhibited high nucleotide diversity generated by point mutation, early-termination, altered start codon, deletion/insertion, and intragenic recombination and is predicted to be an intrinsically disordered protein. AVR2 amino acid sequences conferring a virulent phenotype had a higher disorder tendency in both the N- and C-terminal regions compared with sequences conferring an avirulent phenotype. In addition, we also found virulent AVR2 mutants gained one or two short linear interaction motifs, the critical components of disordered proteins required for protein-protein interactions. Furthermore, virulent AVR2 mutants were predicted to be unstable and have a short protein half-life. Taken together, these results support the notion that intrinsic disorder is important for the effector function of pathogens and demonstrate that SLiM-mediated protein-protein interaction in the C-terminal effector domain might contribute greatly to the evasion of resistance-protein detection in P. infestans.
Keyword ：
AVR2 AVR2 coevolution coevolution effector effector intragenic recombination intragenic recombination intrinsically disordered protein intrinsically disordered protein Phytophthora infestans Phytophthora infestans
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| GB/T 7714 | Yang, Li-Na , Liu, Hao , Duan, Guo-Hua et al. The Phytophthora infestans AVR2 Effector Escapes R2 Recognition Through Effector Disordering [J]. | MOLECULAR PLANT-MICROBE INTERACTIONS , 2020 , 33 (7) : 921-931 . |
| MLA | Yang, Li-Na et al. "The Phytophthora infestans AVR2 Effector Escapes R2 Recognition Through Effector Disordering" . | MOLECULAR PLANT-MICROBE INTERACTIONS 33 . 7 (2020) : 921-931 . |
| APA | Yang, Li-Na , Liu, Hao , Duan, Guo-Hua , Huang, Yan-Mei , Liu, Shiting , Fang, Zhi-Guo et al. The Phytophthora infestans AVR2 Effector Escapes R2 Recognition Through Effector Disordering . | MOLECULAR PLANT-MICROBE INTERACTIONS , 2020 , 33 (7) , 921-931 . |
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Gene flow is an important evolutionary force that enables adaptive responses of plant pathogens in response to changes in the environment and plant disease management strategies. In this study, we made a direct inference concerning gene flow in the Irish famine pathogen Phytophthora infestans between two of its hosts (potato and tomato) as well as between China and India. This was done by comparing sequence characteristics of the eukaryotic translation elongation factor 1 alpha (eEF-1 alpha) gene, generated from 245 P. infestans isolates sampled from two countries and hosts. Consistent with previous results, we found that eEF-1 alpha gene was highly conserved and point mutation was the only mechanism generating any sequence variation. Higher genetic variation was found in the eEF-1 alpha sequences in the P. infestans populations sampled from tomato compared to those sampled from potato. We also found the P. infestans population from India displayed a higher genetic variation in the eEF-1 alpha sequences compared to China. No gene flow was detected between the pathogen populations from the two countries, which is possibly attributed to the geographic barrier caused by Himalaya Plateau and the minimum cross-border trade of potato and tomato products. The implications of these results for a sustainable management of late blight diseases are discussed.
Keyword ：
elongation factor-1 alpha (eEF-1 alpha) elongation factor-1 alpha (eEF-1 alpha) haplotype network haplotype network phylogenetic analysis phylogenetic analysis Phytophthora infestans Phytophthora infestans population connectivity population connectivity population genetic structure population genetic structure
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| GB/T 7714 | Wang, Yan-Ping , Xie, Jia-Hui , Wu, E-Jiao et al. Lack of gene flow between Phytophthora infestans populations of two neighboring countries with the largest potato production [J]. | EVOLUTIONARY APPLICATIONS , 2019 , 13 (2) : 318-329 . |
| MLA | Wang, Yan-Ping et al. "Lack of gene flow between Phytophthora infestans populations of two neighboring countries with the largest potato production" . | EVOLUTIONARY APPLICATIONS 13 . 2 (2019) : 318-329 . |
| APA | Wang, Yan-Ping , Xie, Jia-Hui , Wu, E-Jiao , Yahuza, Lurwanu , Duan, Guo-Hua , Shen, Lin-Lin et al. Lack of gene flow between Phytophthora infestans populations of two neighboring countries with the largest potato production . | EVOLUTIONARY APPLICATIONS , 2019 , 13 (2) , 318-329 . |
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为了探索发酵豆粕对肉鸡生产性能和屠宰性能的影响。1日龄雏鸡250只,随机分为5组,每组5个重复,每个重复10只。Ⅰ组为空白对照组,饲喂基础日粮;Ⅱ组为抗生素对照组,在基础日粮中添加30 mg/kg的杆菌肽锌和6 mg/kg的硫酸抗敌素;Ⅲ、Ⅳ、Ⅴ组分别在基础日粮中用3%、6%、9%的发酵豆粕替代普通豆粕。结果表明:体增重以空白对照组(组Ⅰ)最低,较空白对照组,9%组(组Ⅴ)显著提高(P<0.05),其余各组极显著提高(P<0.01);料重比6%组(组Ⅳ)最低,与空白对照组差异极显著(P<0.01),其他各组间差异均不显著(P>0.05);成活率空白对照组最低,抗生素组(组Ⅱ)、9%组与空白对照组差异极显著(P<0.01),6%组差异显著(P<0.05)。6%组半净膛率显著高于空白对照组和抗生素组(P<0.05);各组全净膛率均高于空白对照组(P<0.05),6%组差异极显著(P<0.01);3%组(组Ⅲ)腿肌率最高,与空白对照组差异极显著(P<0.01),其他各组间差异不显著(P>0.05)。试验结果表明,肉鸡配合饲料中用6%发酵豆粕替代普通豆粕最为适宜,可提高肉鸡生长性能,极显著降低料重比,但与抗生素组相比差异不显著。
Keyword ：
发酵豆粕 发酵豆粕 屠宰性能 屠宰性能 生长性能 生长性能 肉鸡 肉鸡
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| GB/T 7714 | 祁瑞雪 , 王全溪 , 杨玉芬 et al. 发酵豆粕对肉鸡生长性能和屠宰性能的影响 [J]. | 中国农学通报 , 2012 , 28 (14) : 36-39 . |
| MLA | 祁瑞雪 et al. "发酵豆粕对肉鸡生长性能和屠宰性能的影响" . | 中国农学通报 28 . 14 (2012) : 36-39 . |
| APA | 祁瑞雪 , 王全溪 , 杨玉芬 , 刘昊 , 王长康 , 陈庆达 et al. 发酵豆粕对肉鸡生长性能和屠宰性能的影响 . | 中国农学通报 , 2012 , 28 (14) , 36-39 . |
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从福建龙岩蛋鸭原种场选择7个蛋鸭品种(品系)S1、S2、S3、J、P、F、K,组建5组不同的三系杂交配套组合:J♂×(F♂×S1♀)、P♂×(F♂×S3♀)、F♂×(P♂×S2♀)、F♂×(K♂×S2♀)、F♂×(P♂×S3♀).5个配套系商品代经500日饲养试验测定,筛选出生产性能最优的2个配套系为青壳蛋系J♂×(F♂×S1♀)、白壳蛋系F♂×(P♂×S3♀).青壳蛋系的产蛋率达50%时,平均日龄(118±2.45)d,平均体重(1595.6±22.5)g,500日龄的产蛋数、总蛋重、蛋重、料蛋比分别为(330.6±4.29)枚、(22.69±0.21)kg、(72.50±0.20)g、(2.86±0.03)∶1;白壳蛋系的产蛋率达50%时,平均日龄(114.7±1.25)d,平均体重(1526.6±12.31)g,500日龄的产蛋数、总蛋重、蛋重、料蛋比分别为(335.29±1.8)枚、(23.22±0.17)kg、(71.36±0.05)g、(2.77±0.01)∶1.可见,2个配套系的生产性能均达国内领先水平,具有推广应用价值.
Keyword ：
蛋鸭 蛋鸭 选育 选育 配套系 配套系
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| GB/T 7714 | 王长康 , 江宵兵 , 肖天放 et al. 高产蛋鸭配套系的选育 [J]. | 福建农林大学学报(自然科学版) , 2011 , 40 (02) : 178-181 . |
| MLA | 王长康 et al. "高产蛋鸭配套系的选育" . | 福建农林大学学报(自然科学版) 40 . 02 (2011) : 178-181 . |
| APA | 王长康 , 江宵兵 , 肖天放 , 林如龙 , 刘昊 . 高产蛋鸭配套系的选育 . | 福建农林大学学报(自然科学版) , 2011 , 40 (02) , 178-181 . |
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为确定食品和饲料中的猪源性成分,本试验优化了PCR检测猪源性成分的反应体系。试验采用20μl的PCR反应体系,分别测定Taq DNA聚合酶为0U、1U、2U、3U时;25m mol/L的MgCl2为0、2、4、6μl时;dNTP为0、0.1、0.2、0.4μl时,其他PCR反应因素为最大量的结果。结果表明:优化的猪源性成分PCR反应体系中Taq DNA聚合酶、25mmol/L MgCl2、dNTP适宜浓度分别为2U,2μl,0.4μl。优化后的反应体系对猪肉DNA最低检测浓度为0.04ng/μl。本研究优化出的猪源性成分PCR反应体系经二重PCR反应技术进行验证,不仅快速,简便,准确性与灵敏度高,并且经济、高效。
Keyword ：
PCR检测 PCR检测 优化 优化 反应体系 反应体系 猪源性成分 猪源性成分
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| GB/T 7714 | 刘昊 , 连春 , 王长康 et al. 猪源性成分的PCR检测技术优化研究 [J]. | 中国农学通报 , 2009 , 25 (18) : 1-6 . |
| MLA | 刘昊 et al. "猪源性成分的PCR检测技术优化研究" . | 中国农学通报 25 . 18 (2009) : 1-6 . |
| APA | 刘昊 , 连春 , 王长康 , 陈文炳 , 邵碧英 . 猪源性成分的PCR检测技术优化研究 . | 中国农学通报 , 2009 , 25 (18) , 1-6 . |
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选取128头7～8 kg"杜长大"健康断乳仔猪作为试验动物,根据体重相近、公母各半的原则将其随机分为对照组和试验组,每个组设4个重复,每个重复16头,探讨添加免疫增强剂-"巴罗顿"对仔猪生长性能和腹泻率的影响。对照组饲喂基础日粮,试验组在每千克基础日粮中添加2 g巴罗顿。结果表明,在每千克仔猪日粮中添加2 g巴罗顿对仔猪的生长性能没有产生显著影响(P>0.05),有减少仔猪腹泻的趋势。
Keyword ：
仔猪 仔猪 免疫增强剂 免疫增强剂 生长性能 生长性能 腹泻率 腹泻率
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| GB/T 7714 | 刘昊 , 乔建国 , 张文圣 . 免疫增强剂-巴罗顿对断乳仔猪生长性能的影响 [J]. | 福建畜牧兽医 , 2009 , 31 (04) : 6-7 . |
| MLA | 刘昊 et al. "免疫增强剂-巴罗顿对断乳仔猪生长性能的影响" . | 福建畜牧兽医 31 . 04 (2009) : 6-7 . |
| APA | 刘昊 , 乔建国 , 张文圣 . 免疫增强剂-巴罗顿对断乳仔猪生长性能的影响 . | 福建畜牧兽医 , 2009 , 31 (04) , 6-7 . |
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将396只1日龄雄性黄羽肉鸡随机分为4个处理,每处理设3个重复,每重复33只。对照组饲喂基础日粮,试验组分别用2.0%、1.0%和0.5%美洲大蠊虫粉等量取代基础日粮中的鱼粉,进行70d的饲养,分别在第42d、70d测定肉鸡的生长性能、血液生化指标及十二指肠绒毛高度。结果表明,各处理日采食量、日增重及死亡率差异均不显著(P>0.05),2%虫粉组的料重比显著低于对照组8.06%(P<0.05);各处理血清尿素氮、尿酸及白蛋白含量差异均不显著(P>0.05),2%虫粉组血清总蛋白、球蛋白含量显著高于对照组(P<0.05);2%虫粉组及1%虫粉组42d,70d十二指肠绒毛高度均显著高于对照组(P<0.05)。
Keyword ：
小肠绒毛高度 小肠绒毛高度 生产性能 生产性能 生化指标 生化指标 美洲大蠊 美洲大蠊 肉鸡 肉鸡
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| GB/T 7714 | 刘昊 , 李忠荣 , 刘景 et al. 美洲大蠊对肉鸡生产性能、血液生化指标及小肠绒毛高度的影响 [J]. | 北京农学院学报 , 2009 , 24 (01) : 28-32 . |
| MLA | 刘昊 et al. "美洲大蠊对肉鸡生产性能、血液生化指标及小肠绒毛高度的影响" . | 北京农学院学报 24 . 01 (2009) : 28-32 . |
| APA | 刘昊 , 李忠荣 , 刘景 , 王长康 , 周琼 . 美洲大蠊对肉鸡生产性能、血液生化指标及小肠绒毛高度的影响 . | 北京农学院学报 , 2009 , 24 (01) , 28-32 . |
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A proteomic approach was taken to compare the proteomes of normal flowering buds and flowering reversion buds in longan (Dimocarpus longan Lour. cv. Longyou). Two-dimensional gel electrophoresis (2-DE), coupled with mass spectroscopy and protein database searching, recognized 18 proteins that were differentially expressed in flowering reversion buds. Eleven of these were down-regulated, whereas seven were up-regulated. A subset of 13 proteins was identified by MALDI-TOF-TOF/MS and classified into regulatory proteins (kinase, 20S proteasome alpha 6 Subunit, putative alpha 7 proteasome subunit, auxin-induced protein, and abscisic stress ripening-like protein), antioxidant-related proteins (Chain A, GDP-mannose-3',5'-epimerase, putative lactoylglutathione lyase, and Chain A, ascorbate peroxidase), pollen fertility-related proteins (putative leucoanthocyanidin reductase 2, and putative isoflavone reductase), photosynthesis-related proteins (large subunit, ribulose-bisphosphate carboxylase-oxygenase), and molecular chaperones (disulfide isomerase). Among them, regulatory and antioxidant-related proteins accounted for almost two-thirds of these proteins, suggesting that they may play a more important role in bud differentiation. Identification of these proteins provides insights that may lead to a better understanding of the molecular basis for flowering reversion in longan. (C) 2009 Elsevier B.V. All rights reserved.
Keyword ：
2-DE 2-DE Differential proteins Differential proteins Flowering reversion buds Flowering reversion buds Longan Longan Proteomic analysis Proteomic analysis
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| GB/T 7714 | Chen, Sisi , Liu, Hao , Chen, Wei et al. Proteomic analysis of differentially expressed proteins in longan flowering reversion buds [J]. | SCIENTIA HORTICULTURAE , 2009 , 122 (2) : 275-280 . |
| MLA | Chen, Sisi et al. "Proteomic analysis of differentially expressed proteins in longan flowering reversion buds" . | SCIENTIA HORTICULTURAE 122 . 2 (2009) : 275-280 . |
| APA | Chen, Sisi , Liu, Hao , Chen, Wei , Xie, Dongli , Zheng, Shaoquan . Proteomic analysis of differentially expressed proteins in longan flowering reversion buds . | SCIENTIA HORTICULTURAE , 2009 , 122 (2) , 275-280 . |
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选用1日龄AA肉仔鸡共设雄性对照组(Ⅰ)、雄性芦荟粉组(Ⅱ)、雌性对照组(Ⅲ)和雌性芦荟粉组(Ⅳ)4个处理,Ⅰ、Ⅲ组饲喂基础日粮,Ⅱ、Ⅳ组均在基础日粮中添加600 mg.kg-1的芦荟粉。结果表明:雄性芦荟粉组比雄性对照组极显著降低了料肉比(P<0.01);21 d雄性芦荟粉组脾脏指数显著高于对照组(P<0.05);21 d雄性芦荟粉组新城疫血凝抑制效价显著高于对照组(P<0.05);42 d雄性芦荟粉组自然杀伤细胞杀伤活性极显著高于对照组(P<0.01)。说明日粮中添加芦荟粉可以提高肉仔鸡生产性能和免疫机能,且对雄性肉仔鸡生产性能和免疫机能影响效果优于雌性。
Keyword ：
免疫功能 免疫功能 生产性能 生产性能 肉仔鸡 肉仔鸡 芦荟粉 芦荟粉
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| GB/T 7714 | 刘昊 , 佟建明 , 贾洪强 et al. 芦荟粉对不同性别肉仔鸡免疫机能及生产性能的影响 [J]. | 福建农业学报 , 2007 , (03) : 288-292 . |
| MLA | 刘昊 et al. "芦荟粉对不同性别肉仔鸡免疫机能及生产性能的影响" . | 福建农业学报 03 (2007) : 288-292 . |
| APA | 刘昊 , 佟建明 , 贾洪强 , 董晓芳 , 王长康 , 高微微 et al. 芦荟粉对不同性别肉仔鸡免疫机能及生产性能的影响 . | 福建农业学报 , 2007 , (03) , 288-292 . |
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