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学者姓名：周东辉
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Abstract ：
Toxoplasmosis is a zoonotic protozoan disease caused by Toxoplasma gondii. Swine serve as intermediate hosts in the transmission of T. gondii, rendering the detection of this pathogen in swine populations is essential for public health. The chemiluminescence immunoassay (CLIA) is a highly regarded quantitative serological technique recognized for its exceptional sensitivity, specificity, and extensive linear range, thereby facilitating its application in the detection of swine diseases. In this study, we developed CLIA assays using GRA6 (dense granule protein 6), SAG1 (surface antigens 1), and GRA6-SAG1 antigens to detect T. gondii in pigs, achieving results in just 20 minutes. Testing 148 serum samples revealed that the GRA6-SAG1 CLIA had the highest diagnostic performance, with a sensitivity of 97.96 %, specificity of 100.00 %, and an intra-batch coefficient of variation below 8.00 %. Furthermore, analysis of 300 serum samples collected from swine in Fujian province showed the positive rates the T. gondii detection by the GRA6, SAG1, and GRA6-SAG1 CLIA assays is 66.00 %, 68.67 %, and 74.67 %, respectively. The T. gondiii positive rates were higher than those of commercial ELISA kits A (61.00 %) and B (16.33 %) as well as indirect hemagglutination (IHA) kit C (8.33 %). The GRA6-SAG1 CLIA developed in this study presents a rapid, specific, sensitive, and stable method for the detection of antibodies against T. gondii, holding significant potential for the accurate diagnosis of toxoplasmosis in swine.
Keyword ：
Chemiluminescence immunoassay Chemiluminescence immunoassay GRA6 and SAG1 GRA6 and SAG1 Pig Pig Serum Antibody detection Serum Antibody detection Toxoplasma gondii Toxoplasma gondii
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| GB/T 7714 | Wu, Ran , Song, Ya-Fei , Gu, Yanlong et al. A chemiluminescence immunoassay for detection of Toxoplasma gondii antibody in porcine based on GRA6 and SAG1 [J]. | VETERINARY PARASITOLOGY , 2025 , 335 . |
| MLA | Wu, Ran et al. "A chemiluminescence immunoassay for detection of Toxoplasma gondii antibody in porcine based on GRA6 and SAG1" . | VETERINARY PARASITOLOGY 335 (2025) . |
| APA | Wu, Ran , Song, Ya-Fei , Gu, Yanlong , Li, Jiang , Niu, Zhipeng , Zhou, Dong-Hui . A chemiluminescence immunoassay for detection of Toxoplasma gondii antibody in porcine based on GRA6 and SAG1 . | VETERINARY PARASITOLOGY , 2025 , 335 . |
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Cryptosporidium spp. and Giardia duodenalis are two well-known protist pathogens which can result in diarrhea in humans and animals. The two parasites have been detected in humans and dogs worldwide with diverse species and genotypes of various levels and zoonotic potential and public health concern. This investigation aimed to determine the prevalence and molecular characteristics of Cryptosporidium spp. and G. duodenalis in humans and dogs in Fujian province, China. A total of 1149 fecal samples (643 from humans and 506 from dogs) were collected from nine districts in Fujian Province. Detection of Cryptosporidium was performed using nested PCR targeting the SSU rRNA gene, while G. duodenalis was detected by amplification three genes including the beta-giardin, glutamate dehydrogenase, and triosephosphate isomerase. No Cryptosporidium or G. duodenalis were detected in any of the human samples tested. In contrast, the prevalence of Cryptosporidium in dog samples was found to be 1.2 % (6/506), while the infection rate of G. duodenalis was detected in 0.4 % (2/506) of the dog samples. According to the age analysis, all samples infected with Cryptosporidium 2.2 % (6/268) and G. duodenalis 0.8 % (2/268) were from dogs <= 1 year. Sex-based analysis indicated that the infection rate of Cryptosporidium was slightly higher in male dogs (1.2 %, 3/248) compared to female dogs (1.2 %, 3/258). Additionally, G. duodenalis was detected in 0.8 % (2/248) of male dogs, while no positive samples were observed in female dogs. Phylogenetic analyses further identified C. canis, a zoonotic species of Cryptosporidium, as well as two zoonotic assemblages (C and D) of G. duodenalis. These results provide preliminary reference data for monitoring Cryptosporidium and G. duodenalis infections in both humans and dogs, and also offer essential support for future prospective studies.
Keyword ：
Cryptosporidium Cryptosporidium Dog Dog Fujian Province Fujian Province Giardia duodenalis Giardia duodenalis Human Human Zoonotic infection Zoonotic infection
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| GB/T 7714 | Li, Si-Ang , Lin, Yu-Ling , Bai, Yun-Peng et al. Prevalence and molecular characterization of Cryptosporidium spp. and Giardia duodenalis in humans and dogs from Fujian Province, Southeast China [J]. | FOOD AND WATERBORNE PARASITOLOGY , 2025 , 40 . |
| MLA | Li, Si-Ang et al. "Prevalence and molecular characterization of Cryptosporidium spp. and Giardia duodenalis in humans and dogs from Fujian Province, Southeast China" . | FOOD AND WATERBORNE PARASITOLOGY 40 (2025) . |
| APA | Li, Si-Ang , Lin, Yu-Ling , Bai, Yun-Peng , Wen, Fuli , Huang, Li-Yuan , Miao, Wen-Yuan et al. Prevalence and molecular characterization of Cryptosporidium spp. and Giardia duodenalis in humans and dogs from Fujian Province, Southeast China . | FOOD AND WATERBORNE PARASITOLOGY , 2025 , 40 . |
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本发明公开了一种斯氏艾美耳球虫子孢子的纯化方法，包括如下步骤：剖检获取病变兔肝，获得斯氏艾美耳球虫卵囊；进行孢子化处理，获得重铬酸钾孢子化卵囊悬液；向沉淀物加入次氯酸钠后进行冰上吹打；取离心后的上层浮沫和液体，加入清水，离心获得干净孢子化卵囊；加入研磨用玻璃珠震荡处理；一次离心，吸掉上层液体，加入50％percol液，二次离心；向沉淀物中加入脱囊缓冲液，水浴消化处理一定时间；将消化处理后的悬液进行一次离心，取沉淀物，加入55％percol液，二次离心、取沉淀物，稀释，定容，自然过滤得干净的斯氏艾美耳球虫子孢子。其显著效果是：操作简单，费用低，子孢子产量高，纯化过程更省时。
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| GB/T 7714 | 周东辉 , 叶勇刚 , 牛志鹏 et al. 斯氏艾美耳球虫子孢子的纯化方法 : CN202411539264.4[P]. | 2024-10-31 . |
| MLA | 周东辉 et al. "斯氏艾美耳球虫子孢子的纯化方法" : CN202411539264.4. | 2024-10-31 . |
| APA | 周东辉 , 叶勇刚 , 牛志鹏 , 魏勇 , 杨锐 , 吴学婧 . 斯氏艾美耳球虫子孢子的纯化方法 : CN202411539264.4. | 2024-10-31 . |
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Toxoplasma gondii (T. gondii) is an intracellular parasite that extensively infects warm-blooded animals, causing toxoplasmosis and posing a significant threat to global public health. In this study, we investigated the association between T. gondii infection and ferroptosis in host cells, as well as the regulatory role of glutathione peroxidase 4 (GPX4). Our findings revealed that mice infected with RH and PRU strains of T. gondii exhibited significantly elevated levels of reactive oxygen species and malondialdehyde in brain and liver tissues. Concurrently, the expression of GPX4, a critical negative regulator of ferroptosis, was downregulated, which correlated with the elevated parasite burden. In Vero cells, T. gondii infection similarly inhibited GPX4 expression, whereas GPX4 overexpression suppressed T. gondii proliferation. These results indicate that T. gondii infection can promote ferroptosis in host cells and that GPX4 plays a pivotal role in regulating infection and proliferation. This study provides novel insights into the pathogenic mechanisms of T. gondii and identifies GPX4 as a regulatory factor that constrains parasite proliferation, offering new approaches for toxoplasmosis prevention and control.
Keyword ：
ferroptosis ferroptosis glutathione peroxidase 4 (GPX4) glutathione peroxidase 4 (GPX4) lipid peroxidation lipid peroxidation reactive oxygen species (ROS) reactive oxygen species (ROS) Toxoplasma gondii Toxoplasma gondii
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| GB/T 7714 | Gu, Yanlong , Niu, Zhipeng , Lu, Hui-Hong et al. Inhibition of GPX4 by Toxoplasma gondii Promotes Ferroptosis and Enhances Its Proliferation in Acute and Chronic Infection [J]. | CELLS , 2025 , 14 (10) . |
| MLA | Gu, Yanlong et al. "Inhibition of GPX4 by Toxoplasma gondii Promotes Ferroptosis and Enhances Its Proliferation in Acute and Chronic Infection" . | CELLS 14 . 10 (2025) . |
| APA | Gu, Yanlong , Niu, Zhipeng , Lu, Hui-Hong , Li, Si-Ang , Zhou, Dong-Hui . Inhibition of GPX4 by Toxoplasma gondii Promotes Ferroptosis and Enhances Its Proliferation in Acute and Chronic Infection . | CELLS , 2025 , 14 (10) . |
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Background enterocytozoon bieneusiis a cosmopolitan microsporidian that infects a wide range of vertebrate and invertebrate hosts including humans, domestic animals and wild game. In this study, we determined the prevalence of E. bieneusi in pigs from Guangxi Zhuang Autonomous Region, China, examined the different genotypes present, and assessed their zoonotic potential. Methods This study investigate the prevalence and multilocus genotyping of E. bieneusi in pigs from Guangxi Zhuang Autonomous Region in China. We collected 721 fecal samples from pigs in four regions (Guigang, Nanning, Hezhou and Yulin). These samples were subsequently analyzed using nested PCR and multilocus sequence typing (MLST). Results The results demonstrated that the overall prevalence of E. bieneusi in pigs was 24.55%, ranging from 11.48 to 43.26% among four regions. The infection rates of E. bieneusi in pigs of four types (breeding pigs, piglets, nursery pigs and fattening pigs) and two feeding modes (free-range farming and intensive farming) ranged from 9.71 to 42.42%, and 16.71-34.71% respectively. The results of statistical analysis revealed that there were significant differences in the prevalence of E. bieneusi in different regions, types and feeding modes (P < 0.05). Ten novel genotypes (GXP-1 to GXP-10) and 12 known genotypes of E. bieneusi were identified. Genotype EbpC and EbpA were the main prevalent genotypes in this study. All the identified E. bieneusi genotypes were clustered to zoonotic group 1 by phylogenetic analysis. Fifty-seven samples were simultaneously amplified at three microsatellite loci and one minisatellite loci, resulting in the formation of 44 distinct multilocus genotypes (MLGs). Conclusions The present study for the first time revealed the prevalence and genotypes diversity of E. bieneusi in pigs from Guangxi Zhuang Autonomous Region, China, providing foundational data for the prevention and control of this parasitic disease. Moreover, the observed genotype distribution of E. bieneusi suggests a substantial risk of zoonotic transmission, highlighting the need for sustained surveillance and targeted intervention strategies.
Keyword ：
Enterocytozoon bieneusi Enterocytozoon bieneusi Genotype Genotype Guangxi Zhuang autonomous region Guangxi Zhuang autonomous region Multilocus sequence typing Multilocus sequence typing Pigs Pigs Prevalence Prevalence
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| GB/T 7714 | Li, Si-Ang , Liu, Yang , Lu, Hui-Hong et al. First molecular detection and multilocus genotyping of Enterocytozoon bieneusi from pigs in Guangxi Zhuang Autonomous region, Southern China [J]. | BMC VETERINARY RESEARCH , 2025 , 21 (1) . |
| MLA | Li, Si-Ang et al. "First molecular detection and multilocus genotyping of Enterocytozoon bieneusi from pigs in Guangxi Zhuang Autonomous region, Southern China" . | BMC VETERINARY RESEARCH 21 . 1 (2025) . |
| APA | Li, Si-Ang , Liu, Yang , Lu, Hui-Hong , Song, Ya-Fei , Chu, Meng-Jie , Huang, Fei et al. First molecular detection and multilocus genotyping of Enterocytozoon bieneusi from pigs in Guangxi Zhuang Autonomous region, Southern China . | BMC VETERINARY RESEARCH , 2025 , 21 (1) . |
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Giardia duodenalis is a zoonotic parasite that causes gastrointestinal diseases in both humans and animals. To evaluate the prevalence and genetic diversity of G. duodenalis in black goats, we collected 539 fecal samples from nine districts in Fujian Province, China. The presence of G. duodenalis was confirmed through nested PCR targeting the SSU rRNA gene, and genotyping was performed at the beta-giardin, glutamate dehydrogenase, and triosephosphate isomerase loci. Among the samples, 115 tested positive, yielding an overall infection rate of 21.34%. Assemblages A and E were identified, with assemblage E being predominant. Statistical analysis revealed significant regional differences in infection rates (p < 0.01), with Zhangzhou exhibiting the highest infection rate (39%) and Fuzhou the lowest (3.13%). No significant differences in infection rates were observed based on age: 24.56% (56/228) for goats <1 year, 14.92% (27/181) for goats 1-2 years, 26.8% (26/97) for goats 2-3 years, and 18.18% (6/33) for goats >= 3 years. Similarly, no significant differences were found between sexes: 24.84% (40/161) for males and 19.84% (75/378) for females. Notably, assemblage A, a zoonotic genotype, was detected, indicating a potential risk of cross-species transmission. This study contributes to a deeper understanding of G. duodenalis in black goats and provides critical data for the development of targeted control strategies in Fujian Province.
Keyword ：
black goat black goat Fujian Province Fujian Province Giardia duodenalis Giardia duodenalis prevalence prevalence
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| GB/T 7714 | Huang, Shou-Xiao , Hu, Kai , Fu, Peng-Fei et al. Occurrence and Multi-Locus Genotyping of Giardia duodenalis in Black Goats from Fujian Province, China [J]. | ANIMALS , 2025 , 15 (2) . |
| MLA | Huang, Shou-Xiao et al. "Occurrence and Multi-Locus Genotyping of Giardia duodenalis in Black Goats from Fujian Province, China" . | ANIMALS 15 . 2 (2025) . |
| APA | Huang, Shou-Xiao , Hu, Kai , Fu, Peng-Fei , Li, Si-Ang , Liu, Yang , Niu, Zhipeng et al. Occurrence and Multi-Locus Genotyping of Giardia duodenalis in Black Goats from Fujian Province, China . | ANIMALS , 2025 , 15 (2) . |
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As a newly discovered virus, Decapoda iridovirus 1 (DIV1) can cause a mortality rate of up to 100% in crustaceans, leading to huge economic losses. At present, there is no effective prevention and control measures for this disease. In the present study, the specific primers targeting highly conserved regions of MCP gene were designed, and then a quantitative real-time PCR method was established. The results indicate that DIV1 quantitative real-time PCR established has good specificity and does not cross react with other pathogens including white spot syndrome virus (WSSV), infectious subcutaneous and hematopoietic necrosis virus (IHHNV) and Vibrio parahaemolyticus induced acute hepatopancreatic necrosis disease (VpAHPND). The real-time PCR was capable of detecting DIV1 DNA at a minimum concentration of 10 copies/mu L within 34 cycles. The method has good repeatability, with intra group and inter group coefficients of variation both less than 2%. Thirty-two clinical samples were assessed using both the real-time PCR and conventional PCR. The results shown real-time PCR we established are more sensitive than conventional PCR. In conclusion, this method has strong specificity, stable repeatability, and high sensitivity, providing technical support for clinical diagnosis, epidemiology investigation and monitoring of DIV1.
Keyword ：
detection method detection method DIV1 DIV1 MCP gene MCP gene real-time PCR real-time PCR SYBR Green I (R) SYBR Green I (R)
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| GB/T 7714 | Zhao, Fu-Rong , Liu, Yang , Zheng, Qin et al. Development and application of a quantitative real-time PCR method for detection of Decapod iridescent virus 1 [J]. | FRONTIERS IN MICROBIOLOGY , 2024 , 15 . |
| MLA | Zhao, Fu-Rong et al. "Development and application of a quantitative real-time PCR method for detection of Decapod iridescent virus 1" . | FRONTIERS IN MICROBIOLOGY 15 (2024) . |
| APA | Zhao, Fu-Rong , Liu, Yang , Zheng, Qin , Zhang, Yan-Ge , Han, Yijuan , Zhou, Dong-Hui et al. Development and application of a quantitative real-time PCR method for detection of Decapod iridescent virus 1 . | FRONTIERS IN MICROBIOLOGY , 2024 , 15 . |
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Toxoplasma gondii is an important protozoan pathogen, which can cause severe diseases in the newborns and immunocompromised individuals. Developing an effective vaccine against Toxoplasma infection is a critically important global health priority. Immunofluorescence staining analysis revealed that TgSAG2 and TgSRS2 are membrane associated and displayed on the surface of the parasite. Immunizations with pBud-SAG2, pBud-SRS2 and pBud-SAG2-SRS2 DNA vaccines significantly increased the production of specific IgG antibodies. Immunization with pBud-SAG2-SRS2 elicited cellular immune response with higher concentrations of IFN- gamma and IL-4 compared to the control group. Antigen-specific lymphocyte proliferations in the pBud-SRS2 and pBud-SAG2SRS2 groups were significantly higher compared to that in the control group. Furthermore, 30 % of mice immunized with pBud-SAG2-SRS2 survived after the challenge infection with virulent T. gondii RH tachyzoites. This study revealed that immunization with pBud-SAG2-SRS2 induced potent immune responses, and has the potential as a promising vaccine candidate for the control of T. gondii infection.
Keyword ：
DNA vaccine DNA vaccine TgSAG2 TgSAG2 TgSRS2 TgSRS2 Toxoplasma gondii Toxoplasma gondii
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| GB/T 7714 | Guo, Xu-Dong , Zhou, Chun-Xue , Cui, Lin-Lin et al. Evaluation of protective immunity induced by a DNA vaccine encoding SAG2 and SRS2 against Toxoplasma gondii infection in mice [J]. | ACTA TROPICA , 2024 , 257 . |
| MLA | Guo, Xu-Dong et al. "Evaluation of protective immunity induced by a DNA vaccine encoding SAG2 and SRS2 against Toxoplasma gondii infection in mice" . | ACTA TROPICA 257 (2024) . |
| APA | Guo, Xu-Dong , Zhou, Chun-Xue , Cui, Lin-Lin , Qiu, Hui-Jie , Wang, Yong-Liang , Fu, Ming et al. Evaluation of protective immunity induced by a DNA vaccine encoding SAG2 and SRS2 against Toxoplasma gondii infection in mice . | ACTA TROPICA , 2024 , 257 . |
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本实用新型公开了一种虫卵计数板，涉及虫卵计数领域，主要用于解决现有虫卵计数板厚度大且不能同时检测比重大和比重小的虫卵或卵囊的问题。其主要结构为：包括至少两个位于同一水平面且可拆式连接的载玻片，所述载玻片上表面设有一用于水平放置盖玻片的盖玻片安放区，所述盖玻片安放区中部设有样本容纳槽，所述样本容纳槽和盖玻片的相对面上皆设有指示刻线。本实用新型提供了一种虫卵计数板，该装置使用方便，能根据实际需要，同时检测比重大和比重小的虫卵或卵囊。
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| GB/T 7714 | 叶勇刚 , 周东辉 , 李常银 et al. 一种虫卵计数板 : CN202322845622.1[P]. | 2023-10-23 . |
| MLA | 叶勇刚 et al. "一种虫卵计数板" : CN202322845622.1. | 2023-10-23 . |
| APA | 叶勇刚 , 周东辉 , 李常银 , 叶蒲锡源 , 魏勇 , 于吉锋 et al. 一种虫卵计数板 : CN202322845622.1. | 2023-10-23 . |
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Toxoplasma gondii ( T. gondii ) is an intracellular protozoan that can cause toxoplasmosis in all warm-blooded hosts. This study focused on the prevalence and genetic characterize of T. gondii in ducks from Fujian province, China. Genomic DNA was extracted from duck tissue samples (heart, liver, lung, and muscle). To assess the genetic diversity of the T. gondii isolates, it was determined by using multilocus polymerase chain reaction-restriction fragment length polymorphism ( PCR-RFLP ) technology. A total of 586 ducks from 5 cities in Fujian province were tested, and 35 (6.0%) of which were found to be positive for the T. gondii B1 gene. Further genotyping of these positive samples at 10 genetic markers (SAG1, SAG2, SAG3, BTUB, GRA6, c22-8, c29-2, L358, PK1, and Apico) using PCR-RFLP revealed that one tissue samples (heart samples from Fuzhou ducks) were identi fied as Type I (ToxoDB#10). This study is the first report on the prevalence and genetic characterization of T. gondii in ducks in Fujian province, and Type I (ToxoDB#10) is found in ducks in China for the first time. The findings document the genetic characterization of T. gondii in free-range ducks from Fujian Province, thereby enriching the understanding of T. gondii genetic diversity in China. Moreover, these results provide essential data support for further prospective studies and underscores the "One Health " concept, emphasizing the integral link among human, animal, and environmental health.
Keyword ：
China China duck duck genetic characterization genetic characterization prevalence prevalence Toxoplasma gondii Toxoplasma gondii
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| GB/T 7714 | Li, Si-Ang , Huang, Li-Yuan , Guo, Xu-Dong et al. First identified Toxoplasma gondii Type I in market-sold ducks in Fujian province, China: a significant for public health [J]. | POULTRY SCIENCE , 2024 , 103 (9) . |
| MLA | Li, Si-Ang et al. "First identified Toxoplasma gondii Type I in market-sold ducks in Fujian province, China: a significant for public health" . | POULTRY SCIENCE 103 . 9 (2024) . |
| APA | Li, Si-Ang , Huang, Li-Yuan , Guo, Xu-Dong , Miao, Wen-Yuan , Lin, Ying-Sheng , Zhou, Dong-Hui . First identified Toxoplasma gondii Type I in market-sold ducks in Fujian province, China: a significant for public health . | POULTRY SCIENCE , 2024 , 103 (9) . |
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